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Gene Dmel\Hsp67Bc

General Information
Symbol	Dmel\Hsp67Bc	Species	D. melanogaster
Name	Heat shock gene 67Bc	Annotation symbol	CG4190
Feature type	protein_coding_gene	FlyBase ID	FBgn0001229
Gene Model Status	Current	Stock availability	6 publicly available
Also Known As	gene 3
Genomic Location
Chromosome (arm)	3L	Recombination map
Cytogenetic map	67B2-67B2	Sequence location	3L:9,364,611..9,365,583 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Heat shock gene 67Bc is referred to in FlyBase by the symbol Dmel\Hsp67Bc (CG4190, FBgn0001229). It is a protein_coding_gene from Drosophila melanogaster. Its molecular function is unknown. There is experimental evidence that it is involved in the biological process: protein lipidation; response to methotrexate; regulation of translational initiation by eIF2 alpha phosphorylation; regulation of autophagy. 5 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide. Protein features are: Alpha crystallin/Heat shock protein; HSP20-like chaperone; Heat shock protein Hsp20. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of very low expression. Peak expression observed during late larval stages, at stages throughout the pupal period. Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: larval Malpighian tubules. Expression at moderate levels in the following post-embryonic organs or tissues: larval/adult central nervous system, larval hindgut, adult Malpighian tubules. Comments on Affy2 ProbeSet: ProbeSet 1638484_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of Hsp67Bc. Gene sequence location is 3L:9364611..9365583.
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	Hsp22 - Hsp-G3 There are seven closely related heat-shock genes at 67B (Ayme and Tissieres, 1985; Pauli, Arrigo, Vasquez, Tonka, and Tissieres, 1989, Genome 31: 671-76). In addition to the four small heat-shock genes previously identified (Hsp22, Hsp23, Hsp26, and Hsp27), three more genes (Hsp-G1, Hsp-G2, and Hsp-G3, formerly called Gene1, Gene2, and Gene3) have been found clustered within 15 kb of DNA at the same 67B cytological location. All seven genes are heat-shock inducible in almost all cells at the stages tested (Ayme and Tissieres, 1985). The genes are also transcribed during certain developmental stages in the absence of heat shock (Sirotkin and Davidson, 1982, Dev. Biol. 89: 196-210). Pauli et al (1989) report that the maximum accumulation of developmental rRNA in a majority of these small heat-shock genes occurs in the white pupae stage; in Hsp-G2, however, a small transcipt is found in embryos, first and second instar larvae, and young pupae; and a larger transcript in the pupal and adult stages of males (Pauli and Tonka, 1987, J. Mol. Biol. 198: 235-40; Pauli, Tonka, and Ayme-Southgate, 1988, J. Mol. Biol. 200: 47-53). In absence of stress, the expression of Hsp26 has been observed in spermatocytes, nurse cells, epithelium, imaginal discs, proventriculus, and neurocytes (Glaser, Wolfner, and Lis, 1986, EMBO 5: 747-54). Transcripts of Hsp26 and Hsp27 accumulate in adult ovaries, apparently originating in nurse cells (Zimmerman, Petri, and Meselson, 1983, Cell 32: 1161-70).
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2012_01	References Mosqueira et al., 2010, PLoS ONE 5(10): e13450 Sequence features BKN23739 HFA11193
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 67B2-67B2   Limits computationally determined from genome sequence between P{PZ}l(3)0162901629&P{PZ}mRpL1210534 and P{EP}Hsp26EP3336&P{EP}Hsp26EP3315
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Hsp67Bc for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Hsp67Bc-RA FBtr0076497  NM_079270   973   199
Additional Transcript Data & Comments
Reported size (kB)	2.3, 1.6, 1.075 (northern blot) (Pauli and Tonka, 1987)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Hsp67Bc-PA   FBpp0076225   22.2   199   6.57     NP_523994   AAF50292
Additional Polypeptide Data & Comments
Reported size (kDa)	169 (aa); 18.792 (kD predicted) (Pauli and Tonka, 1987)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Alpha crystallin/Heat shock protein (IPR001436) Heat shock protein Hsp20 (IPR002068) HSP20-like chaperone (IPR008978)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE014296 AAF50292   AY231947   BP542186   BP550569   BT023289   CA803785   CA805074   CA805406   CA806416   CA807160   CA807273   CA807579   CO183580   CO185004   CO190192   CO194208   CO297559   CO312336   EC088766   EC213585   EC229919   EC234331   EL870432   EL870434   EL870437   GH960332   X06542 CAA29788
UniProtKB/Swiss-Prot	P22979
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
northern blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage 11 -- 17     (Pauli and Tonka, 1987) Comment:reference states >=7 hr AEL larval stage -- pupal stage     (Pauli and Tonka, 1987) prepupal stage     (Pauli et al., 1989)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0001229    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0001229 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of very low expression. Peak expression observed during late larval stages, at stages throughout the pupal period. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0001229 expression level Developmental Stage   Expression Level embryo 00-02hr    35 embryo 02-04hr    53 embryo 04-06hr    70 embryo 06-08hr    634 embryo 08-10hr    1660 embryo 10-12hr    3697 embryo 12-14hr    3613 embryo 14-16hr    3357 embryo 16-18hr    3155 embryo 18-20hr    3150 embryo 20-22hr    2775 embryo 22-24hr    2808 larva L1    1934 larva L2    550 larva L3 12hr old    276 larva L3 puffstage 1-2    531 larva L3 puffstage 3-6    2873 larva L3 puffstage 7-9    12330 white prepupae new    20492 white prepupae 12hr    20769 white prepupae 24hr    11574 pupae 2d postWPP    28322 pupae 3d postWPP    7968 pupae 4d postWPP    7495 adult male 01day    363 adult male 05day    386 adult male 30day    279 adult female 01day    98 adult female 05day    50 adult female 30day    38 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr    35 embryo 02-04hr    53 embryo 04-06hr    70 embryo 06-08hr  (634) embryo 08-10hr  (1660) embryo 10-12hr  (3697) embryo 12-14hr  (3613) embryo 14-16hr  (3357) embryo 16-18hr  (3155) embryo 18-20hr  (3150) embryo 20-22hr  (2775) embryo 22-24hr  (2808) larva L1  (1934) larva L2  (550) larva L3 12hr old  (276) larva L3 puffstage 1-2  (531) larva L3 puffstage 3-6  (2873) larva L3 puffstage 7-9  (12330) white prepupae new  (20492) white prepupae 12hr  (20769) white prepupae 24hr  (11574) pupae 2d postWPP  (28322) pupae 3d postWPP  (7968) pupae 4d postWPP  (7495) adult male 01day  (363) adult male 05day  (386) adult male 30day  (279) adult female 01day    98 adult female 05day    50 adult female 30day    38 Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    35 embryo 02-04hr    53 embryo 04-06hr    70 embryo 06-08hr    634 embryo 08-10hr  (1660) embryo 10-12hr  (3697) embryo 12-14hr  (3613) embryo 14-16hr  (3357) embryo 16-18hr  (3155) embryo 18-20hr  (3150) embryo 20-22hr  (2775) embryo 22-24hr  (2808) larva L1  (1934) larva L2    550 larva L3 12hr old    276 larva L3 puffstage 1-2    531 larva L3 puffstage 3-6  (2873) larva L3 puffstage 7-9  (12330) white prepupae new  (20492) white prepupae 12hr  (20769) white prepupae 24hr  (11574) pupae 2d postWPP  (28322) pupae 3d postWPP  (7968) pupae 4d postWPP  (7495) adult male 01day    363 adult male 05day    386 adult male 30day    279 adult female 01day    98 adult female 05day    50 adult female 30day    38 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    35 embryo 02-04hr    53 embryo 04-06hr    70 embryo 06-08hr    634 embryo 08-10hr    1660 embryo 10-12hr    3697 embryo 12-14hr    3613 embryo 14-16hr    3357 embryo 16-18hr    3155 embryo 18-20hr    3150 embryo 20-22hr    2775 embryo 22-24hr    2808 larva L1    1934 larva L2    550 larva L3 12hr old    276 larva L3 puffstage 1-2    531 larva L3 puffstage 3-6    2873 larva L3 puffstage 7-9  (12330) white prepupae new  (20492) white prepupae 12hr  (20769) white prepupae 24hr  (11574) pupae 2d postWPP  (28322) pupae 3d postWPP    7968 pupae 4d postWPP    7495 adult male 01day    363 adult male 05day    386 adult male 30day    279 adult female 01day    98 adult female 05day    50 adult female 30day    38 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    35 embryo 02-04hr    53 embryo 04-06hr    70 embryo 06-08hr    634 embryo 08-10hr    1660 embryo 10-12hr    3697 embryo 12-14hr    3613 embryo 14-16hr    3357 embryo 16-18hr    3155 embryo 18-20hr    3150 embryo 20-22hr    2775 embryo 22-24hr    2808 larva L1    1934 larva L2    550 larva L3 12hr old    276 larva L3 puffstage 1-2    531 larva L3 puffstage 3-6    2873 larva L3 puffstage 7-9    12330 white prepupae new    20492 white prepupae 12hr    20769 white prepupae 24hr    11574 pupae 2d postWPP    28322 pupae 3d postWPP    7968 pupae 4d postWPP    7495 adult male 01day    363 adult male 05day    386 adult male 30day    279 adult female 01day    98 adult female 05day    50 adult female 30day    38 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0001229 expression level Developmental Stage   Expression Level embryo 00-02hr    35 embryo 02-04hr    53 embryo 04-06hr    70 embryo 06-08hr    634 embryo 08-10hr    1660 embryo 10-12hr    3697 embryo 12-14hr    3613 embryo 14-16hr    3357 embryo 16-18hr    3155 embryo 18-20hr    3150 embryo 20-22hr    2775 embryo 22-24hr    2808 larva L1    1934 larva L2    550 larva L3 12hr old    276 larva L3 puffstage 1-2    531 larva L3 puffstage 3-6    2873 larva L3 puffstage 7-9    12330 white prepupae new    20492 white prepupae 12hr    20769 white prepupae 24hr    11574 pupae 2d postWPP    28322 pupae 3d postWPP    7968 pupae 4d postWPP    7495 adult male 01day    363 adult male 05day    386 adult male 30day    279 adult female 01day    98 adult female 05day    50 adult female 30day    38 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr    35 embryo 02-04hr    53 embryo 04-06hr    70 embryo 06-08hr  (634) embryo 08-10hr  (1660) embryo 10-12hr  (3697) embryo 12-14hr  (3613) embryo 14-16hr  (3357) embryo 16-18hr  (3155) embryo 18-20hr  (3150) embryo 20-22hr  (2775) embryo 22-24hr  (2808) larva L1  (1934) larva L2  (550) larva L3 12hr old  (276) larva L3 puffstage 1-2  (531) larva L3 puffstage 3-6  (2873) larva L3 puffstage 7-9  (12330) white prepupae new  (20492) white prepupae 12hr  (20769) white prepupae 24hr  (11574) pupae 2d postWPP  (28322) pupae 3d postWPP  (7968) pupae 4d postWPP  (7495) adult male 01day  (363) adult male 05day  (386) adult male 30day  (279) adult female 01day    98 adult female 05day    50 adult female 30day    38 Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    35 embryo 02-04hr    53 embryo 04-06hr    70 embryo 06-08hr    634 embryo 08-10hr  1660 embryo 10-12hr  (3697) embryo 12-14hr  (3613) embryo 14-16hr  (3357) embryo 16-18hr  (3155) embryo 18-20hr  (3150) embryo 20-22hr  (2775) embryo 22-24hr  (2808) larva L1  1934 larva L2    550 larva L3 12hr old    276 larva L3 puffstage 1-2    531 larva L3 puffstage 3-6  (2873) larva L3 puffstage 7-9  (12330) white prepupae new  (20492) white prepupae 12hr  (20769) white prepupae 24hr  (11574) pupae 2d postWPP  (28322) pupae 3d postWPP  (7968) pupae 4d postWPP  (7495) adult male 01day    363 adult male 05day    386 adult male 30day    279 adult female 01day    98 adult female 05day    50 adult female 30day    38 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    35 embryo 02-04hr    53 embryo 04-06hr    70 embryo 06-08hr    634 embryo 08-10hr    1660 embryo 10-12hr    3697 embryo 12-14hr    3613 embryo 14-16hr    3357 embryo 16-18hr    3155 embryo 18-20hr    3150 embryo 20-22hr    2775 embryo 22-24hr    2808 larva L1    1934 larva L2    550 larva L3 12hr old    276 larva L3 puffstage 1-2    531 larva L3 puffstage 3-6    2873 larva L3 puffstage 7-9  12330 white prepupae new  20492 white prepupae 12hr  (20769) white prepupae 24hr  11574 pupae 2d postWPP  (28322) pupae 3d postWPP    7968 pupae 4d postWPP    7495 adult male 01day    363 adult male 05day    386 adult male 30day    279 adult female 01day    98 adult female 05day    50 adult female 30day    38 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    35 embryo 02-04hr    53 embryo 04-06hr    70 embryo 06-08hr    634 embryo 08-10hr    1660 embryo 10-12hr    3697 embryo 12-14hr    3613 embryo 14-16hr    3357 embryo 16-18hr    3155 embryo 18-20hr    3150 embryo 20-22hr    2775 embryo 22-24hr    2808 larva L1    1934 larva L2    550 larva L3 12hr old    276 larva L3 puffstage 1-2    531 larva L3 puffstage 3-6    2873 larva L3 puffstage 7-9    12330 white prepupae new    20492 white prepupae 12hr    20769 white prepupae 24hr    11574 pupae 2d postWPP    28322 pupae 3d postWPP    7968 pupae 4d postWPP    7495 adult male 01day    363 adult male 05day    386 adult male 30day    279 adult female 01day    98 adult female 05day    50 adult female 30day    38 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0001229    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0001229 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: larval Malpighian tubules. Expression at moderate levels in the following post-embryonic organs or tissues: larval/adult central nervous system, larval hindgut, adult Malpighian tubules. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0001229 expression level Tissue   Expression Level Larval Central Nervous System    236.35 Larval Midgut    70 Larval Hindgut    134.7 Larval Malpighian Tubules    6057.2 Larval Fat Body    47 Larval Salivary Gland    43 Larval Trachea no informative data Larval Carcass    30.95 Adult Head    41.7 Adult Eye    5.1 Adult Brain    211.3 Adult Thoracic-Abdominal Ganglion    57.5 Adult Crop    7.9 Adult Midgut    8.3 Adult Hindgut    5.2 Adult Malpighian Tubules    258.1 Adult Fat Body    8.4 Adult Salivary Gland no informative data Adult Heart    4.925 Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca    8.3 Adult Ovary    1.7 Adult Testis    18 Adult Male Accessory Gland    9.8 Adult Carcass    5.8 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    236.35 Larval Midgut    70 Larval Hindgut    134.7 Larval Malpighian Tubules  (6057.2) Larval Fat Body    47 Larval Salivary Gland    43 Larval Trachea no informative data Larval Carcass    30.95 Adult Head    41.7 Adult Eye    5.1 Adult Brain    211.3 Adult Thoracic-Abdominal Ganglion    57.5 Adult Crop    7.9 Adult Midgut    8.3 Adult Hindgut    5.2 Adult Malpighian Tubules    258.1 Adult Fat Body    8.4 Adult Salivary Gland no informative data Adult Heart    4.925 Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca    8.3 Adult Ovary    1.7 Adult Testis    18 Adult Male Accessory Gland    9.8 Adult Carcass    5.8 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    236.35 Larval Midgut    70 Larval Hindgut    134.7 Larval Malpighian Tubules  (6057.2) Larval Fat Body    47 Larval Salivary Gland    43 Larval Trachea no informative data Larval Carcass    30.95 Adult Head    41.7 Adult Eye    5.1 Adult Brain    211.3 Adult Thoracic-Abdominal Ganglion    57.5 Adult Crop    7.9 Adult Midgut    8.3 Adult Hindgut    5.2 Adult Malpighian Tubules    258.1 Adult Fat Body    8.4 Adult Salivary Gland no informative data Adult Heart    4.925 Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca    8.3 Adult Ovary    1.7 Adult Testis    18 Adult Male Accessory Gland    9.8 Adult Carcass    5.8 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    236.35 Larval Midgut    70 Larval Hindgut    134.7 Larval Malpighian Tubules    6057.2 Larval Fat Body    47 Larval Salivary Gland    43 Larval Trachea no informative data Larval Carcass    30.95 Adult Head    41.7 Adult Eye    5.1 Adult Brain    211.3 Adult Thoracic-Abdominal Ganglion    57.5 Adult Crop    7.9 Adult Midgut    8.3 Adult Hindgut    5.2 Adult Malpighian Tubules    258.1 Adult Fat Body    8.4 Adult Salivary Gland no informative data Adult Heart    4.925 Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca    8.3 Adult Ovary    1.7 Adult Testis    18 Adult Male Accessory Gland    9.8 Adult Carcass    5.8 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0001229 expression level Tissue   Expression Level Larval Central Nervous System    236.35 Larval Midgut    70 Larval Hindgut    134.7 Larval Malpighian Tubules    6057.2 Larval Fat Body    47 Larval Salivary Gland    43 Larval Trachea no informative data Larval Carcass    30.95 Adult Head    41.7 Adult Eye    5.1 Adult Brain    211.3 Adult Thoracic-Abdominal Ganglion    57.5 Adult Crop    7.9 Adult Midgut    8.3 Adult Hindgut    5.2 Adult Malpighian Tubules    258.1 Adult Fat Body    8.4 Adult Salivary Gland no informative data Adult Heart    4.925 Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca    8.3 Adult Ovary    1.7 Adult Testis    18 Adult Male Accessory Gland    9.8 Adult Carcass    5.8 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    236.35 Larval Midgut    70 Larval Hindgut    134.7 Larval Malpighian Tubules  (6057.2) Larval Fat Body    47 Larval Salivary Gland    43 Larval Trachea no informative data Larval Carcass    30.95 Adult Head    41.7 Adult Eye    5.1 Adult Brain    211.3 Adult Thoracic-Abdominal Ganglion    57.5 Adult Crop    7.9 Adult Midgut    8.3 Adult Hindgut    5.2 Adult Malpighian Tubules    258.1 Adult Fat Body    8.4 Adult Salivary Gland no informative data Adult Heart    4.925 Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca    8.3 Adult Ovary    1.7 Adult Testis    18 Adult Male Accessory Gland    9.8 Adult Carcass    5.8 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    236.35 Larval Midgut    70 Larval Hindgut    134.7 Larval Malpighian Tubules  (6057.2) Larval Fat Body    47 Larval Salivary Gland    43 Larval Trachea no informative data Larval Carcass    30.95 Adult Head    41.7 Adult Eye    5.1 Adult Brain    211.3 Adult Thoracic-Abdominal Ganglion    57.5 Adult Crop    7.9 Adult Midgut    8.3 Adult Hindgut    5.2 Adult Malpighian Tubules    258.1 Adult Fat Body    8.4 Adult Salivary Gland no informative data Adult Heart    4.925 Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca    8.3 Adult Ovary    1.7 Adult Testis    18 Adult Male Accessory Gland    9.8 Adult Carcass    5.8 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    236.35 Larval Midgut    70 Larval Hindgut    134.7 Larval Malpighian Tubules    6057.2 Larval Fat Body    47 Larval Salivary Gland    43 Larval Trachea no informative data Larval Carcass    30.95 Adult Head    41.7 Adult Eye    5.1 Adult Brain    211.3 Adult Thoracic-Abdominal Ganglion    57.5 Adult Crop    7.9 Adult Midgut    8.3 Adult Hindgut    5.2 Adult Malpighian Tubules    258.1 Adult Fat Body    8.4 Adult Salivary Gland no informative data Adult Heart    4.925 Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca    8.3 Adult Ovary    1.7 Adult Testis    18 Adult Male Accessory Gland    9.8 Adult Carcass    5.8 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea no informative data Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland no informative data Adult Heart     Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    41.7   NA  Eye    5.1   NA  Brain    211.3   236.35  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    57.5   NA  Crop    7.9   70  Midgut    8.3   134.7  Hindgut    5.2   6057.2  Malpighian Tubules    258.1   47  Fat Body    8.4   43  Salivary Gland    no informative data   NA  Heart    4.925   no informative data  Trachea    NA   NA  VirginFemale Spermatheca    no informative data   NA  InseminatedFemale Spermatheca    8.3   NA  Ovary    1.7   NA  Testis    18   NA  Male Accessory Gland    9.8   30.95  Carcass    5.8 modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_25
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0001229 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG4190-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable Hsp67Bcd06009 viable, with Scer\GAL4pnr-MD237 Hsp67BcGD11244 Sterility Allele fertile Hsp67Bcd06009 Phenotype manifest in Allele
Classical Alleles ( 1 )
For All Classical Alleles Show
Allele of Hsp67Bc Class Mutagen Stocks Known lesion Hsp67Bcd06009 P-element activity 2 --
Alleles Carried on Transgenic Constructs ( 4 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Hsp67Bc Class Mutagen Stocks Known lesion Hsp67BcGD11244 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes Hsp67BcGL00377 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Hsp67BcKK103547 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Hsp67BcScer\UAS.T:SV5\V5 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes
Aneuploid Aberrations
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD11244} NA P{KK103547} NA P{TRiP.GL00377} NA P{UAS-Hsp67Bc.V5} NA heat-shock construct P{UAS-Hsp67Bc.V5} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{XP}Hsp67Bcd06009 NA
Gene Ontology: Function, Process & Cellular Component ( 5 unique terms )
Terms Based on Experimental Evidence ( 4 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
protein lipidation inferred from mutant phenotype (Carra et al., 2010) regulation of autophagy inferred from mutant phenotype (Carra et al., 2010) regulation of translational initiation by eIF2 alpha phosphorylation inferred from mutant phenotype (Carra et al., 2010) response to methotrexate inferred from expression pattern (Chahine and O'Donnell, 2010)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 1 term )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
response to heat inferred from sequence or structural similarity with EMBL:U96099 (FlyBase, 1992-) non-traceable author statement (Swiss-Prot Project Members, 1991.8.1)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Hsp67Bc allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 64468 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0001229 DroID - A comprehensive database of gene and protein interactions. • FBgn0001229 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 39071
Orthologs
Genome-wide drosophilid orthologs	Dana\GF23740 Dere\GG14048 Dgri\GH16985 Dmoj\GI13089 Dper\GL22633 Dpse\GA24923 Dsec\GM24882 Dsim\GD12934 Dvir\GJ13837 Dwil\GK19253 Dyak\Hsp67Bc
Curated drosophilid orthologs	Dyak\Hsp67Bc
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • CBP01228 Caenorhabditis briggsae (nematode) CBP04459 Caenorhabditis briggsae (nematode) RP17678 Caenorhabditis remanei (nematode) RP23178 Caenorhabditis remanei (nematode) RP25015 Caenorhabditis remanei (nematode) 69964 Lottia gigantea (owl limpet) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • FBgn0068140 FBgn0221251 FBgn0201056 FBgn0184659 FBgn0179744 FBgn0246306 FBgn0160224 FBgn0135846 FBgn0124456 FBgn0106310 FBgn0100734
Stocks & Reagents
Stocks Listed in FlyBase ( 6 )
Bloomington	19227 w1118; P{XP}Hsp67Bcd06009 35452 y1 sc* v1; P{TRiP.GL00377}attP2/TM3, Sb1
Harvard	- P{XP}Hsp67Bcd06009
VDRC	v103974 P{KK103547}VIE-260B v26416 w1118; P{GD11244}v26416 v26417 w1118; P{GD11244}v26417
Genomic Clones ( 1 )
	BACR01E20 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 26 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	IP02523
Other clones	DPiM_IP02523 yak-em_Hsp67Bc
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones
Other clones	53075 101768 145189 CK01.58B.A8 EC25987 EC29101 EC37611 EK046936 EK170061 EK228512 ESG013b.E21_B01 ESG016c.E21_B08 ESG017b.E21_B02 ESG018c.E21.1_D04 ESG0110c.E21.1_C07 ESG0116b.B21_G01 ESG0118a.B21_D07 FGC010A08 FGM207H05 IP02521 IP02522 IP02524 RP004546748
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0001229 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 39071
Antibody Information
	polyclonal (Carra et al., 2010)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments
Other Comments
	In unshocked cells Hsp83 is moderately transcribed while transcription from the other heat shock genes is undetectable. Engaged but paused RNA molecules are found at the various Hsp70 and Hsp26 genes but not at the other heat shock genes. Increased transcription of the heat shock genes is observed within 1-2 mins of heat shock and maximal rates were reached within 2-5 minutes. Rates of transcription vary over a 20-fold range. (Vazquez et al., 1993) Exposure of cells to pulses of elevated temperature initiates the heat-shock response. A restricted subset of genes, the Hsp genes, is activated and the majority of transcription and translation is shut down. 3H-uridine incorporation ceases at its usual positions and commences at new puff sites. Preexisting polysomes disaggregate and within a few minutes a new population of polysomes appears containing newly transcribed mRNA; this RNA hybridizes to some of the heat-shock puffs. Similar response inducible by other stressful treatments. The response may be elicited at all stages of the life cycle and in cultured cells. (Lindsley and Zimm, 1992) Activation of transcription of Hsp genes apparently involves the sequential binding of two or more protein factors in vicinity of TATA box. (Wu, 1984) Polymerase II dissociates from most chromosome regions and accumulates at the new heat shock puff sites upon heat shock. (Bonner and Kerby, 1982) The binding sites for the protein factors required for activation of transcription of Hsp genes are multiple short upstream sequence elements called HSEs or heat shock consensus elements. (Pelham, 1982, Xiao and Lis, 1988) Hsp67Bc is transcribed during certain developmental stages in the absence of heat shock. (Sirotkin and Davidson, 1982) The effects of heat shock may be abrogated to some degree by pretreatment with a pulse of a slightly lower temperature. (Mitchell et al., 1979, Petersen and Mitchell, 1981) Mitochondrial and histone-gene activities persist transcription and translation. (Spradling et al., 1977) In polytene cells, during heat shock response, existing puffs regress and a novel group quickly appear at 33B, 63C, 64F, 67B, 70A, 87A, 87C, 93D, 95D. (Tissieres et al., 1974) The heat shock response follows a pulse of 36oC to 40oC; treatments above 40oC inhibit all activity and lead to death; treatments of 30oC-35oC induce heat-shock-protein synthesis without repressing normal protein synthesis. (Tissieres et al., 1974) In polytene cells, during heat shock response, existing puffs regress and a novel group quickly appear at cytological locations 33B, 63C, 64F, 67B, 70A, 87A, 87C, 93D, 95D. (Ashburner, 1970)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_079270 RefSeq (Proteins) • NP_523994 DDBJ / EMBL / GenBank • AAF50292 CAA29788 X06542 Entrez Gene - A searchable database of RefSeq genes. • 39071 UniProtKB/Swiss-Prot • P22979
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Alpha crystallin/Heat shock protein (IPR001436) Heat shock protein Hsp20 (IPR002068) HSP20-like chaperone (IPR008978)
Linkouts
	BioGRID - A database of protein and genetic interactions • 64468 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0001229 DroID - A comprehensive database of gene and protein interactions. • FBgn0001229 DRSC - Results from RNAi screens. • FBgn0001229 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0001229 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG4190-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0001229 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 39071 InParanoid A subset of ortholog calls from InParanoid. • CBP01228 Caenorhabditis briggsae (nematode) CBP04459 Caenorhabditis briggsae (nematode) RP17678 Caenorhabditis remanei (nematode) RP23178 Caenorhabditis remanei (nematode) RP25015 Caenorhabditis remanei (nematode) 69964 Lottia gigantea (owl limpet) InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 39071 modMine - Data generated by the modENCODE project. • FBgn0001229 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • FBgn0068140 FBgn0221251 FBgn0201056 FBgn0184659 FBgn0179744 FBgn0246306 FBgn0160224 FBgn0135846 FBgn0124456 FBgn0106310 FBgn0100734
Synonyms & Secondary IDs ( 18 )
Reported As
Symbol Synonym	CG4190 (Whitfield, 2001.1.5, Keleman et al., 2009.8.5, Carra et al., 2010, Chahine and O'Donnell, 2010, Ni et al., 2011.7.19) Dm-HSP67Bc (Carra et al., 2010) gene3 (Southgate et al., 1985) gene 3 (Vazquez et al., 1993, Vazquez, 1991, Pauli et al., 1989, Pauli et al., 1988, Ayme-Southgate and Tissieres, 1985) gene-3   gene5 (Sirotkin and Davidson, 1982) HSP67 (Chahine and O'Donnell, 2010) hsp67B (Ringrose et al., 2003) Hsp67Bc (Sudi et al., 2008, Harvey et al., 2008, Bettencourt et al., 2008, Birch-Machin et al., 2005, Xi et al., 2008, Takahashi et al., 2010, Carra et al., 2010) HSP67Bc (Carra et al., 2010, Chahine and O'Donnell, 2010) Hsp67BC (Mosqueira et al., 2010) HSPB8 (Carra et al., 2010) Hspg3 (Maroni, 1994) Hsp-G3   small hsp locus 67B (Yao et al., 2007) unnamed (Pauli, 1999.9.9)
Name Synonym	Gene 3 (Pauli and Tonka, 1987) Heat shock gene 67Bc
Secondary FlyBase IDs
References ( 50 )
Generate a list of	All references relating to this gene ( 50 )
List References by type	Research paper  ( 34 ) Supplementary material  ( 3 ) Personal communication to FlyBase  ( 5 ) FlyBase analysis  ( 1 ) DNA/RNA sequence record  ( 1 ) Book  ( 1 ) Protein sequence record  ( 1 ) Computer file  ( 1 ) Curated genome annotation  ( 1 ) Automatic genome annotation  ( 1 )
Recent research papers ( 5 )
	Blanco et al., 2010, BMC Dev. Biol. 10: 94 Gene expression following induction of regeneration in Drosophila wing imaginal discs. Expression profile of regenerating wing discs. [FBrf0211812] Carra et al., 2010, J. Biol. Chem. 285(48): 37811--37822 Identification of the Drosophila Ortholog of HSPB8: IMPLICATION OF HSPB8 LOSS OF FUNCTION IN PROTEIN FOLDING DISEASES. [FBrf0212431] Chahine and O'Donnell, 2010, Arch. Insect Biochem. Physiol. 73(3): 128--147 Effects of acute or chronic exposure to dietary organic anions on secretion of methotrexate and salicylate by Malpighian tubules of Drosophila melanogaster larvae. [FBrf0214072] Mosqueira et al., 2010, PLoS ONE 5(10): e13450 Chronic Hypoxia Impairs Muscle Function in the Drosophila Model of Duchenne's Muscular Dystrophy (DMD). [FBrf0212137] Takahashi et al., 2010, BMC Evol. Biol. 10: 284 Effects of small Hsp genes on developmental stability and microenvironmental canalization. [FBrf0211941] Show all research papers ...