• Home
• Tools
  • Tools Overview
  • Query Tools

    
    • QuickSearch
    • QueryBuilder
    • TermLink
    • CytoSearch
    • RNA-Seq Search
    • Interactions Browser
    • ImageBrowse
    • Find A Person
    • Google™ FlyBase
  • Genomic/Map Tools

    
    • BLAST
    • GBrowse
    • GBrowse 2 Beta
    • CytoSearch
    • Feature Mapper
    • Aberrations Maps
    • Chromosome Maps
    • Coordinates Converter
  • Retrieve/Convert

    
    • Batch Download
    • Coordinates Converter
    • ID Converter
  • Data Submission

    
    • Fast-Track Your Paper
    • Submit Personal Comm
    • Add A New Person
    • Update Person Info
  • People

    
    • Find A Person
    • Add A New Person
    • Update Person Info
• Files
  • Files Overview
  • Current release
  • Archived releases
  • Map Conversion
  • FTP site:
  • Releases (FTP)
  • Genomes (FTP)
• Species
  • Phylogeny
  • Sequenced Species
  • Synteny Table
  • Color Illustrations
  • Abbreviations
• Documents
  • About FlyBase
  • Reference Manual:
  • Sections
  • Contents
  • Nomenclature
  • Release Notes
• Resources
  • All Resources
  • Model Organisms
  • Stock Collections
  • Vectors & Constructs
  • Other links:
  • BDGP
  • DGRC
  • DIS by issue
  • FlyExpress
  • Interactive Fly
  • modENCODE
  • Textpresso for Fly
• News
  • News
  • FlyBase Forum
  • FlyBase Positions
  • Meetings
  • Courses
  • Fly Board
  • White papers
  • Funding
  • bionet.dros
• Help
  • Google™ FlyBase
  • Site Map
  • FAQs
  • FlyBase Guides
  • Video Tutorials
  • Report help
  • New to Flies
  • Pers. comm.
  • Author guidelines
  • Contact FlyBase
• Archives
  • Prior Release
  • Other Archives
  • Coordinate Converter

Gene Dmel\Hsp67Bc

General Information
Symbol	Dmel\Hsp67Bc	Species	D. melanogaster
Name	Heat shock gene 67Bc	Annotation symbol	CG4190
Feature type	protein_coding_gene	FlyBase ID	FBgn0001229
Gene Model Status	Current	Stock availability	7 publicly available
Also Known As	gene 3
Genomic Location
Chromosome (arm)	3L	Recombination map
Cytogenetic map	67B2-67B2	Sequence location	3L:9,364,611..9,365,597 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Heat shock gene 67Bc is referred to in FlyBase by the symbol Dmel\Hsp67Bc (CG4190, FBgn0001229). It is a protein_coding_gene from Drosophila melanogaster. Its molecular function is unknown. There is experimental evidence that it is involved in the biological process: protein lipidation; response to methotrexate; regulation of translational initiation by eIF2 alpha phosphorylation; regulation of autophagy. 7 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide. Protein features are: Alpha crystallin/Heat shock protein; Alpha crystallin/Hsp20 domain; HSP20-like chaperone. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of very low expression. Peak expression observed during late larval stages, at stages throughout the pupal period. Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: larval Malpighian tubules. Expression at moderate levels in the following post-embryonic organs or tissues: larval/adult central nervous system, larval hindgut, adult Malpighian tubules. Comments on Affy2 ProbeSet: ProbeSet 1638484_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of Hsp67Bc. Gene sequence location is 3L:9364611..9365597.
User Contributed Data
	Beta version, feedback welcome
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	Hsp22 - Hsp-G3 There are seven closely related heat-shock genes at 67B (Ayme and Tissieres, 1985; Pauli, Arrigo, Vasquez, Tonka, and Tissieres, 1989, Genome 31: 671-76). In addition to the four small heat-shock genes previously identified (Hsp22, Hsp23, Hsp26, and Hsp27), three more genes (Hsp-G1, Hsp-G2, and Hsp-G3, formerly called Gene1, Gene2, and Gene3) have been found clustered within 15 kb of DNA at the same 67B cytological location. All seven genes are heat-shock inducible in almost all cells at the stages tested (Ayme and Tissieres, 1985). The genes are also transcribed during certain developmental stages in the absence of heat shock (Sirotkin and Davidson, 1982, Dev. Biol. 89: 196-210). Pauli et al (1989) report that the maximum accumulation of developmental rRNA in a majority of these small heat-shock genes occurs in the white pupae stage; in Hsp-G2, however, a small transcipt is found in embryos, first and second instar larvae, and young pupae; and a larger transcript in the pupal and adult stages of males (Pauli and Tonka, 1987, J. Mol. Biol. 198: 235-40; Pauli, Tonka, and Ayme-Southgate, 1988, J. Mol. Biol. 200: 47-53). In absence of stress, the expression of Hsp26 has been observed in spermatocytes, nurse cells, epithelium, imaginal discs, proventriculus, and neurocytes (Glaser, Wolfner, and Lis, 1986, EMBO 5: 747-54). Transcripts of Hsp26 and Hsp27 accumulate in adult ovaries, apparently originating in nurse cells (Zimmerman, Petri, and Meselson, 1983, Cell 32: 1161-70).
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 67B2-67B2   Limits computationally determined from genome sequence between P{PZ}l(3)0162901629&P{PZ}mRpL1210534 and P{EP}Hsp26EP3336&P{EP}Hsp26EP3315
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Hsp67Bc for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.46
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Hsp67Bc-RA FBtr0076497  NM_079270   987   199
Additional Transcript Data & Comments
Reported size (kB)	2.3, 1.6, 1.075 (northern blot) (Pauli and Tonka, 1987)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Hsp67Bc-PA   FBpp0076225   22.2   199   6.57     NP_523994   AAF50292
Additional Polypeptide Data & Comments
Reported size (kDa)	169 (aa); 18.792 (kD predicted) (Pauli and Tonka, 1987)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Alpha crystallin/Heat shock protein (IPR001436) Alpha crystallin/Hsp20 domain (IPR002068) HSP20-like chaperone (IPR008978)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE014296 AAF50292   AY231947   BP542186   BP550569   BT023289   CA803785   CA805074   CA805406   CA806416   CA807160   CA807273   CA807579   CO183580   CO185004   CO190192   CO194208   CO297559   CO312336   EC088766   EC213585   EC229919   EC234331   EL870432   EL870434   EL870437   GH960332   X06542 CAA29788
UniProtKB/Swiss-Prot	P22979
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 4 -- 6   organism | faint & ubiquitous   (Fisher et al., 2012) embryonic stage 11 -- 12   yolk nucleus   (Fisher et al., 2012) embryonic stage 13 -- 16   embryonic brain   (Fisher et al., 2012) ventral nerve cord   (Fisher et al., 2012) yolk nucleus   (Fisher et al., 2012) dorsal sensory complex proper primordium   (Fisher et al., 2012) Comment:reported as dorsal/lateral sensory complexes embryonic head sensory system   (Fisher et al., 2012) ventral sensory complex primordium   (Fisher et al., 2012) northern blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage 11 -- 17     (Pauli and Tonka, 1987) Comment:reference states >=7 hr AEL larval stage -- pupal stage     (Pauli and Tonka, 1987) prepupal stage     (Pauli et al., 1989)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: larval Malpighian tubules. Expression at moderate levels in the following post-embryonic organs or tissues: larval/adult central nervous system, larval hindgut, adult Malpighian tubules. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    236.35 Larval Midgut    70 Larval Hindgut    134.7 Larval Malpighian Tubules    6057.2 Larval Fat Body    47 Larval Salivary Gland    43 Larval Trachea    14.125 Larval Carcass    30.95 Adult Head    41.7 Adult Eye    5.1 Adult Brain    211.3 Adult Thoracic-Abdominal Ganglion    57.5 Adult Crop    7.9 Adult Midgut    8.3 Adult Hindgut    5.2 Adult Malpighian Tubules    258.1 Adult Fat Body    8.4 Adult Salivary Gland    11.7 Adult Heart    4.925 Adult VirginFemale Spermatheca    10.6 Adult InseminatedFemale Spermatheca    8.3 Adult Ovary    1.7 Adult Testis    18 Adult Male Accessory Gland    9.8 Adult Carcass    5.8 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    236.35 Larval Midgut    70 Larval Hindgut    134.7 Larval Malpighian Tubules  (6057.2) Larval Fat Body    47 Larval Salivary Gland    43 Larval Trachea    14.125 Larval Carcass    30.95 Adult Head    41.7 Adult Eye    5.1 Adult Brain    211.3 Adult Thoracic-Abdominal Ganglion    57.5 Adult Crop    7.9 Adult Midgut    8.3 Adult Hindgut    5.2 Adult Malpighian Tubules    258.1 Adult Fat Body    8.4 Adult Salivary Gland    11.7 Adult Heart    4.925 Adult VirginFemale Spermatheca    10.6 Adult InseminatedFemale Spermatheca    8.3 Adult Ovary    1.7 Adult Testis    18 Adult Male Accessory Gland    9.8 Adult Carcass    5.8 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    236.35 Larval Midgut    70 Larval Hindgut    134.7 Larval Malpighian Tubules  (6057.2) Larval Fat Body    47 Larval Salivary Gland    43 Larval Trachea    14.125 Larval Carcass    30.95 Adult Head    41.7 Adult Eye    5.1 Adult Brain    211.3 Adult Thoracic-Abdominal Ganglion    57.5 Adult Crop    7.9 Adult Midgut    8.3 Adult Hindgut    5.2 Adult Malpighian Tubules    258.1 Adult Fat Body    8.4 Adult Salivary Gland    11.7 Adult Heart    4.925 Adult VirginFemale Spermatheca    10.6 Adult InseminatedFemale Spermatheca    8.3 Adult Ovary    1.7 Adult Testis    18 Adult Male Accessory Gland    9.8 Adult Carcass    5.8 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    236.35 Larval Midgut    70 Larval Hindgut    134.7 Larval Malpighian Tubules    6057.2 Larval Fat Body    47 Larval Salivary Gland    43 Larval Trachea    14.125 Larval Carcass    30.95 Adult Head    41.7 Adult Eye    5.1 Adult Brain    211.3 Adult Thoracic-Abdominal Ganglion    57.5 Adult Crop    7.9 Adult Midgut    8.3 Adult Hindgut    5.2 Adult Malpighian Tubules    258.1 Adult Fat Body    8.4 Adult Salivary Gland    11.7 Adult Heart    4.925 Adult VirginFemale Spermatheca    10.6 Adult InseminatedFemale Spermatheca    8.3 Adult Ovary    1.7 Adult Testis    18 Adult Male Accessory Gland    9.8 Adult Carcass    5.8 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    236.35 Larval Midgut    70 Larval Hindgut    134.7 Larval Malpighian Tubules    6057.2 Larval Fat Body    47 Larval Salivary Gland    43 Larval Trachea    14.125 Larval Carcass    30.95 Adult Head    41.7 Adult Eye    5.1 Adult Brain    211.3 Adult Thoracic-Abdominal Ganglion    57.5 Adult Crop    7.9 Adult Midgut    8.3 Adult Hindgut    5.2 Adult Malpighian Tubules    258.1 Adult Fat Body    8.4 Adult Salivary Gland    11.7 Adult Heart    4.925 Adult VirginFemale Spermatheca    10.6 Adult InseminatedFemale Spermatheca    8.3 Adult Ovary    1.7 Adult Testis    18 Adult Male Accessory Gland    9.8 Adult Carcass    5.8 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    236.35 Larval Midgut    70 Larval Hindgut    134.7 Larval Malpighian Tubules  (6057.2) Larval Fat Body    47 Larval Salivary Gland    43 Larval Trachea    14.125 Larval Carcass    30.95 Adult Head    41.7 Adult Eye    5.1 Adult Brain    211.3 Adult Thoracic-Abdominal Ganglion    57.5 Adult Crop    7.9 Adult Midgut    8.3 Adult Hindgut    5.2 Adult Malpighian Tubules    258.1 Adult Fat Body    8.4 Adult Salivary Gland    11.7 Adult Heart    4.925 Adult VirginFemale Spermatheca    10.6 Adult InseminatedFemale Spermatheca    8.3 Adult Ovary    1.7 Adult Testis    18 Adult Male Accessory Gland    9.8 Adult Carcass    5.8 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    236.35 Larval Midgut    70 Larval Hindgut    134.7 Larval Malpighian Tubules  (6057.2) Larval Fat Body    47 Larval Salivary Gland    43 Larval Trachea    14.125 Larval Carcass    30.95 Adult Head    41.7 Adult Eye    5.1 Adult Brain    211.3 Adult Thoracic-Abdominal Ganglion    57.5 Adult Crop    7.9 Adult Midgut    8.3 Adult Hindgut    5.2 Adult Malpighian Tubules    258.1 Adult Fat Body    8.4 Adult Salivary Gland    11.7 Adult Heart    4.925 Adult VirginFemale Spermatheca    10.6 Adult InseminatedFemale Spermatheca    8.3 Adult Ovary    1.7 Adult Testis    18 Adult Male Accessory Gland    9.8 Adult Carcass    5.8 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    236.35 Larval Midgut    70 Larval Hindgut    134.7 Larval Malpighian Tubules    6057.2 Larval Fat Body    47 Larval Salivary Gland    43 Larval Trachea    14.125 Larval Carcass    30.95 Adult Head    41.7 Adult Eye    5.1 Adult Brain    211.3 Adult Thoracic-Abdominal Ganglion    57.5 Adult Crop    7.9 Adult Midgut    8.3 Adult Hindgut    5.2 Adult Malpighian Tubules    258.1 Adult Fat Body    8.4 Adult Salivary Gland    11.7 Adult Heart    4.925 Adult VirginFemale Spermatheca    10.6 Adult InseminatedFemale Spermatheca    8.3 Adult Ovary    1.7 Adult Testis    18 Adult Male Accessory Gland    9.8 Adult Carcass    5.8 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    41.7   NA  Eye    5.1   NA  Brain    211.3   236.35  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    57.5   NA  Crop    7.9   70  Midgut    8.3   134.7  Hindgut    5.2   6057.2  Malpighian Tubules    258.1   47  Fat Body    8.4   43  Salivary Gland    no informative data   NA  Heart    4.925   no informative data  Trachea    NA   NA  VirginFemale Spermatheca    no informative data   NA  InseminatedFemale Spermatheca    8.3   NA  Ovary    1.7   NA  Testis    18   NA  Male Accessory Gland    9.8   30.95  Carcass    5.8 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    17 central nervous system, larvae L3    95 central nervous system, pupae P8    845 head, virgin 1-day female    7 head, virgin 4-day female    3 head, virgin 20-day female    2 head, mated 1-day female    5 head, mated 4-day female    4 head, mated 20-day female    5 head, mated 1-day male    7 head, mated 4-day male    9 head, mated 20-day male    5 salivary gland, larvae L3 wandering    52 salivary gland, white prepupae    964 digestive system, larvae L3 wandering    132 digestive system, 1-day adult    5 digestive system, 4-day adult    3 digestive system, 20-day adult    2 fat body, larvae L3 wandering    0 fat body, white prepupae    26 fat body, pupae P8    209 carcass, larvae L3 wandering    25 carcass, 1-day adult    3 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    1 accessory gland, mated 4-day male    13 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    17 central nervous system, larvae L3  (95) central nervous system, pupae P8  (845) head, virgin 1-day female    7 head, virgin 4-day female    3 head, virgin 20-day female    2 head, mated 1-day female    5 head, mated 4-day female    4 head, mated 20-day female    5 head, mated 1-day male    7 head, mated 4-day male    9 head, mated 20-day male    5 salivary gland, larvae L3 wandering  (52) salivary gland, white prepupae  (964) digestive system, larvae L3 wandering  (132) digestive system, 1-day adult    5 digestive system, 4-day adult    3 digestive system, 20-day adult    2 fat body, larvae L3 wandering    0 fat body, white prepupae  26 fat body, pupae P8  (209) carcass, larvae L3 wandering    25 carcass, 1-day adult    3 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    1 accessory gland, mated 4-day male    13 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    17 central nervous system, larvae L3    95 central nervous system, pupae P8  (845) head, virgin 1-day female    7 head, virgin 4-day female    3 head, virgin 20-day female    2 head, mated 1-day female    5 head, mated 4-day female    4 head, mated 20-day female    5 head, mated 1-day male    7 head, mated 4-day male    9 head, mated 20-day male    5 salivary gland, larvae L3 wandering    52 salivary gland, white prepupae  (964) digestive system, larvae L3 wandering  (132) digestive system, 1-day adult    5 digestive system, 4-day adult    3 digestive system, 20-day adult    2 fat body, larvae L3 wandering    0 fat body, white prepupae    26 fat body, pupae P8  (209) carcass, larvae L3 wandering    25 carcass, 1-day adult    3 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    1 accessory gland, mated 4-day male    13 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    17 central nervous system, larvae L3    95 central nervous system, pupae P8    845 head, virgin 1-day female    7 head, virgin 4-day female    3 head, virgin 20-day female    2 head, mated 1-day female    5 head, mated 4-day female    4 head, mated 20-day female    5 head, mated 1-day male    7 head, mated 4-day male    9 head, mated 20-day male    5 salivary gland, larvae L3 wandering    52 salivary gland, white prepupae    964 digestive system, larvae L3 wandering    132 digestive system, 1-day adult    5 digestive system, 4-day adult    3 digestive system, 20-day adult    2 fat body, larvae L3 wandering    0 fat body, white prepupae    26 fat body, pupae P8    209 carcass, larvae L3 wandering    25 carcass, 1-day adult    3 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    1 accessory gland, mated 4-day male    13 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    17 central nervous system, larvae L3    95 central nervous system, pupae P8    845 head, virgin 1-day female    7 head, virgin 4-day female    3 head, virgin 20-day female    2 head, mated 1-day female    5 head, mated 4-day female    4 head, mated 20-day female    5 head, mated 1-day male    7 head, mated 4-day male    9 head, mated 20-day male    5 salivary gland, larvae L3 wandering    52 salivary gland, white prepupae    964 digestive system, larvae L3 wandering    132 digestive system, 1-day adult    5 digestive system, 4-day adult    3 digestive system, 20-day adult    2 fat body, larvae L3 wandering    0 fat body, white prepupae    26 fat body, pupae P8    209 carcass, larvae L3 wandering    25 carcass, 1-day adult    3 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    1 accessory gland, mated 4-day male    13 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    17 central nervous system, larvae L3  (95) central nervous system, pupae P8  (845) head, virgin 1-day female    7 head, virgin 4-day female    3 head, virgin 20-day female    2 head, mated 1-day female    5 head, mated 4-day female    4 head, mated 20-day female    5 head, mated 1-day male    7 head, mated 4-day male    9 head, mated 20-day male    5 salivary gland, larvae L3 wandering  (52) salivary gland, white prepupae  (964) digestive system, larvae L3 wandering  (132) digestive system, 1-day adult    5 digestive system, 4-day adult    3 digestive system, 20-day adult    2 fat body, larvae L3 wandering    0 fat body, white prepupae  26 fat body, pupae P8  (209) carcass, larvae L3 wandering    25 carcass, 1-day adult    3 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    1 accessory gland, mated 4-day male    13 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    17 central nervous system, larvae L3    95 central nervous system, pupae P8  (845) head, virgin 1-day female    7 head, virgin 4-day female    3 head, virgin 20-day female    2 head, mated 1-day female    5 head, mated 4-day female    4 head, mated 20-day female    5 head, mated 1-day male    7 head, mated 4-day male    9 head, mated 20-day male    5 salivary gland, larvae L3 wandering    52 salivary gland, white prepupae  (964) digestive system, larvae L3 wandering  132 digestive system, 1-day adult    5 digestive system, 4-day adult    3 digestive system, 20-day adult    2 fat body, larvae L3 wandering    0 fat body, white prepupae    26 fat body, pupae P8  (209) carcass, larvae L3 wandering    25 carcass, 1-day adult    3 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    1 accessory gland, mated 4-day male    13 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    17 central nervous system, larvae L3    95 central nervous system, pupae P8    845 head, virgin 1-day female    7 head, virgin 4-day female    3 head, virgin 20-day female    2 head, mated 1-day female    5 head, mated 4-day female    4 head, mated 20-day female    5 head, mated 1-day male    7 head, mated 4-day male    9 head, mated 20-day male    5 salivary gland, larvae L3 wandering    52 salivary gland, white prepupae    964 digestive system, larvae L3 wandering    132 digestive system, 1-day adult    5 digestive system, 4-day adult    3 digestive system, 20-day adult    2 fat body, larvae L3 wandering    0 fat body, white prepupae    26 fat body, pupae P8    209 carcass, larvae L3 wandering    25 carcass, 1-day adult    3 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    1 accessory gland, mated 4-day male    13 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of very low expression. Peak expression observed during late larval stages, at stages throughout the pupal period. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    1 embryo 04-06hr    1 embryo 06-08hr    6 embryo 08-10hr    17 embryo 10-12hr    35 embryo 12-14hr    36 embryo 14-16hr    31 embryo 16-18hr    31 embryo 18-20hr    29 embryo 20-22hr    27 embryo 22-24hr    29 larva L1    18 larva L2    5 larva L3 12hr old    3 larva L3 puffstage 1-2    5 larva L3 puffstage 3-6    28 larva L3 puffstage 7-9    123 white prepupae new    201 white prepupae 12hr    205 white prepupae 24hr    115 pupae 2d postWPP    276 pupae 3d postWPP    77 pupae 4d postWPP    69 adult male 01day    4 adult male 05day    4 adult male 30day    3 adult female 01day    1 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    1 embryo 04-06hr    1 embryo 06-08hr    6 embryo 08-10hr    17 embryo 10-12hr  (35) embryo 12-14hr  (36) embryo 14-16hr  (31) embryo 16-18hr  (31) embryo 18-20hr  (29) embryo 20-22hr  (27) embryo 22-24hr  (29) larva L1    18 larva L2    5 larva L3 12hr old    3 larva L3 puffstage 1-2    5 larva L3 puffstage 3-6  (28) larva L3 puffstage 7-9  (123) white prepupae new  (201) white prepupae 12hr  (205) white prepupae 24hr  (115) pupae 2d postWPP  (276) pupae 3d postWPP  (77) pupae 4d postWPP  (69) adult male 01day    4 adult male 05day    4 adult male 30day    3 adult female 01day    1 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    1 embryo 04-06hr    1 embryo 06-08hr    6 embryo 08-10hr    17 embryo 10-12hr    35 embryo 12-14hr    36 embryo 14-16hr    31 embryo 16-18hr    31 embryo 18-20hr    29 embryo 20-22hr    27 embryo 22-24hr    29 larva L1    18 larva L2    5 larva L3 12hr old    3 larva L3 puffstage 1-2    5 larva L3 puffstage 3-6    28 larva L3 puffstage 7-9  (123) white prepupae new  (201) white prepupae 12hr  (205) white prepupae 24hr  (115) pupae 2d postWPP  (276) pupae 3d postWPP    77 pupae 4d postWPP    69 adult male 01day    4 adult male 05day    4 adult male 30day    3 adult female 01day    1 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    1 embryo 04-06hr    1 embryo 06-08hr    6 embryo 08-10hr    17 embryo 10-12hr    35 embryo 12-14hr    36 embryo 14-16hr    31 embryo 16-18hr    31 embryo 18-20hr    29 embryo 20-22hr    27 embryo 22-24hr    29 larva L1    18 larva L2    5 larva L3 12hr old    3 larva L3 puffstage 1-2    5 larva L3 puffstage 3-6    28 larva L3 puffstage 7-9    123 white prepupae new    201 white prepupae 12hr    205 white prepupae 24hr    115 pupae 2d postWPP    276 pupae 3d postWPP    77 pupae 4d postWPP    69 adult male 01day    4 adult male 05day    4 adult male 30day    3 adult female 01day    1 adult female 05day    0 adult female 30day    0 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    1 embryo 04-06hr    1 embryo 06-08hr    6 embryo 08-10hr    17 embryo 10-12hr    35 embryo 12-14hr    36 embryo 14-16hr    31 embryo 16-18hr    31 embryo 18-20hr    29 embryo 20-22hr    27 embryo 22-24hr    29 larva L1    18 larva L2    5 larva L3 12hr old    3 larva L3 puffstage 1-2    5 larva L3 puffstage 3-6    28 larva L3 puffstage 7-9    123 white prepupae new    201 white prepupae 12hr    205 white prepupae 24hr    115 pupae 2d postWPP    276 pupae 3d postWPP    77 pupae 4d postWPP    69 adult male 01day    4 adult male 05day    4 adult male 30day    3 adult female 01day    1 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    1 embryo 04-06hr    1 embryo 06-08hr    6 embryo 08-10hr    17 embryo 10-12hr  (35) embryo 12-14hr  (36) embryo 14-16hr  31 embryo 16-18hr  31 embryo 18-20hr  29 embryo 20-22hr  27 embryo 22-24hr  29 larva L1    18 larva L2    5 larva L3 12hr old    3 larva L3 puffstage 1-2    5 larva L3 puffstage 3-6  28 larva L3 puffstage 7-9  (123) white prepupae new  (201) white prepupae 12hr  (205) white prepupae 24hr  (115) pupae 2d postWPP  (276) pupae 3d postWPP  (77) pupae 4d postWPP  (69) adult male 01day    4 adult male 05day    4 adult male 30day    3 adult female 01day    1 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    1 embryo 04-06hr    1 embryo 06-08hr    6 embryo 08-10hr    17 embryo 10-12hr    35 embryo 12-14hr    36 embryo 14-16hr    31 embryo 16-18hr    31 embryo 18-20hr    29 embryo 20-22hr    27 embryo 22-24hr    29 larva L1    18 larva L2    5 larva L3 12hr old    3 larva L3 puffstage 1-2    5 larva L3 puffstage 3-6    28 larva L3 puffstage 7-9  123 white prepupae new  (201) white prepupae 12hr  (205) white prepupae 24hr  115 pupae 2d postWPP  (276) pupae 3d postWPP    77 pupae 4d postWPP    69 adult male 01day    4 adult male 05day    4 adult male 30day    3 adult female 01day    1 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    1 embryo 04-06hr    1 embryo 06-08hr    6 embryo 08-10hr    17 embryo 10-12hr    35 embryo 12-14hr    36 embryo 14-16hr    31 embryo 16-18hr    31 embryo 18-20hr    29 embryo 20-22hr    27 embryo 22-24hr    29 larva L1    18 larva L2    5 larva L3 12hr old    3 larva L3 puffstage 1-2    5 larva L3 puffstage 3-6    28 larva L3 puffstage 7-9    123 white prepupae new    201 white prepupae 12hr    205 white prepupae 24hr    115 pupae 2d postWPP    276 pupae 3d postWPP    77 pupae 4d postWPP    69 adult male 01day    4 adult male 05day    4 adult male 30day    3 adult female 01day    1 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    2 Schneider line 2 Sg4    2 embryonic 1182-4H    0 embryonic GM2    3 embryonic Kc167    18 embryonic S1    43 embryonic S3    3 leg disc CME L1    4 wing disc CME-W2    15 wing disc ML-DmD8    1 wing disc ML-DmD9    6 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    1 eye-antennal disc ML-DmD11    1 antennal disc ML-DmD20-c5    2 mixed discs ML-DmD4-c1    7 CNS ML-DmBG1-c1    2 CNS ML-DmBG2-c2    19 tumorous blood cells mbn2    2 ovary fGS/OSS    123 ovary OSC    15 ovary OSS    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    2 Schneider line 2 Sg4    2 embryonic 1182-4H    0 embryonic GM2    3 embryonic Kc167    18 embryonic S1  (43) embryonic S3    3 leg disc CME L1    4 wing disc CME-W2    15 wing disc ML-DmD8    1 wing disc ML-DmD9    6 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    1 eye-antennal disc ML-DmD11    1 antennal disc ML-DmD20-c5    2 mixed discs ML-DmD4-c1    7 CNS ML-DmBG1-c1    2 CNS ML-DmBG2-c2    19 tumorous blood cells mbn2    2 ovary fGS/OSS  (123) ovary OSC    15 ovary OSS    9 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    2 Schneider line 2 Sg4    2 embryonic 1182-4H    0 embryonic GM2    3 embryonic Kc167    18 embryonic S1    43 embryonic S3    3 leg disc CME L1    4 wing disc CME-W2    15 wing disc ML-DmD8    1 wing disc ML-DmD9    6 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    1 eye-antennal disc ML-DmD11    1 antennal disc ML-DmD20-c5    2 mixed discs ML-DmD4-c1    7 CNS ML-DmBG1-c1    2 CNS ML-DmBG2-c2    19 tumorous blood cells mbn2    2 ovary fGS/OSS  (123) ovary OSC    15 ovary OSS    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    2 Schneider line 2 Sg4    2 embryonic 1182-4H    0 embryonic GM2    3 embryonic Kc167    18 embryonic S1    43 embryonic S3    3 leg disc CME L1    4 wing disc CME-W2    15 wing disc ML-DmD8    1 wing disc ML-DmD9    6 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    1 eye-antennal disc ML-DmD11    1 antennal disc ML-DmD20-c5    2 mixed discs ML-DmD4-c1    7 CNS ML-DmBG1-c1    2 CNS ML-DmBG2-c2    19 tumorous blood cells mbn2    2 ovary fGS/OSS    123 ovary OSC    15 ovary OSS    9 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    2 Schneider line 2 Sg4    2 embryonic 1182-4H    0 embryonic GM2    3 embryonic Kc167    18 embryonic S1    43 embryonic S3    3 leg disc CME L1    4 wing disc CME-W2    15 wing disc ML-DmD8    1 wing disc ML-DmD9    6 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    1 eye-antennal disc ML-DmD11    1 antennal disc ML-DmD20-c5    2 mixed discs ML-DmD4-c1    7 CNS ML-DmBG1-c1    2 CNS ML-DmBG2-c2    19 tumorous blood cells mbn2    2 ovary fGS/OSS    123 ovary OSC    15 ovary OSS    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    2 Schneider line 2 Sg4    2 embryonic 1182-4H    0 embryonic GM2    3 embryonic Kc167    18 embryonic S1  (43) embryonic S3    3 leg disc CME L1    4 wing disc CME-W2    15 wing disc ML-DmD8    1 wing disc ML-DmD9    6 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    1 eye-antennal disc ML-DmD11    1 antennal disc ML-DmD20-c5    2 mixed discs ML-DmD4-c1    7 CNS ML-DmBG1-c1    2 CNS ML-DmBG2-c2    19 tumorous blood cells mbn2    2 ovary fGS/OSS  (123) ovary OSC    15 ovary OSS    9 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    2 Schneider line 2 Sg4    2 embryonic 1182-4H    0 embryonic GM2    3 embryonic Kc167    18 embryonic S1    43 embryonic S3    3 leg disc CME L1    4 wing disc CME-W2    15 wing disc ML-DmD8    1 wing disc ML-DmD9    6 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    1 eye-antennal disc ML-DmD11    1 antennal disc ML-DmD20-c5    2 mixed discs ML-DmD4-c1    7 CNS ML-DmBG1-c1    2 CNS ML-DmBG2-c2    19 tumorous blood cells mbn2    2 ovary fGS/OSS  123 ovary OSC    15 ovary OSS    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    2 Schneider line 2 Sg4    2 embryonic 1182-4H    0 embryonic GM2    3 embryonic Kc167    18 embryonic S1    43 embryonic S3    3 leg disc CME L1    4 wing disc CME-W2    15 wing disc ML-DmD8    1 wing disc ML-DmD9    6 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    1 eye-antennal disc ML-DmD11    1 antennal disc ML-DmD20-c5    2 mixed discs ML-DmD4-c1    7 CNS ML-DmBG1-c1    2 CNS ML-DmBG2-c2    19 tumorous blood cells mbn2    2 ovary fGS/OSS    123 ovary OSC    15 ovary OSS    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    56 cold shock, 4-day adult    49 heat shock, 4-day adult    48 Cadmium 50 mM 6 hrs, larvae L3    10 Cadmium 50 mM 12 hrs, larvae L3    56 Cadmium 50 mM 48 hrs, 4-day adult    6 Cadmium 100 mM 48 hrs, 4-day adult    28 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    5 Zinc 5 mM 12 hrs, larvae L3    21 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    49 Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    7 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    0 Caffeine 25 mg/ml 48 hrs, 4-day adult    19 Paraquat 5 mM 48 hrs, 4-day adult    2 Paraquat 10 mM 48 hrs, 4-day adult    2 Rotenone 2 μg 12 hrs, larvae L3    9 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  (56) cold shock, 4-day adult  (49) heat shock, 4-day adult  (48) Cadmium 50 mM 6 hrs, larvae L3    10 Cadmium 50 mM 12 hrs, larvae L3  (56) Cadmium 50 mM 48 hrs, 4-day adult    6 Cadmium 100 mM 48 hrs, 4-day adult  (28) Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    5 Zinc 5 mM 12 hrs, larvae L3    21 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3  (49) Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    7 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    0 Caffeine 25 mg/ml 48 hrs, 4-day adult    19 Paraquat 5 mM 48 hrs, 4-day adult    2 Paraquat 10 mM 48 hrs, 4-day adult    2 Rotenone 2 μg 12 hrs, larvae L3    9 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    56 cold shock, 4-day adult    49 heat shock, 4-day adult    48 Cadmium 50 mM 6 hrs, larvae L3    10 Cadmium 50 mM 12 hrs, larvae L3    56 Cadmium 50 mM 48 hrs, 4-day adult    6 Cadmium 100 mM 48 hrs, 4-day adult    28 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    5 Zinc 5 mM 12 hrs, larvae L3    21 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    49 Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    7 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    0 Caffeine 25 mg/ml 48 hrs, 4-day adult    19 Paraquat 5 mM 48 hrs, 4-day adult    2 Paraquat 10 mM 48 hrs, 4-day adult    2 Rotenone 2 μg 12 hrs, larvae L3    9 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    56 cold shock, 4-day adult    49 heat shock, 4-day adult    48 Cadmium 50 mM 6 hrs, larvae L3    10 Cadmium 50 mM 12 hrs, larvae L3    56 Cadmium 50 mM 48 hrs, 4-day adult    6 Cadmium 100 mM 48 hrs, 4-day adult    28 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    5 Zinc 5 mM 12 hrs, larvae L3    21 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    49 Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    7 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    0 Caffeine 25 mg/ml 48 hrs, 4-day adult    19 Paraquat 5 mM 48 hrs, 4-day adult    2 Paraquat 10 mM 48 hrs, 4-day adult    2 Rotenone 2 μg 12 hrs, larvae L3    9 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    56 cold shock, 4-day adult    49 heat shock, 4-day adult    48 Cadmium 50 mM 6 hrs, larvae L3    10 Cadmium 50 mM 12 hrs, larvae L3    56 Cadmium 50 mM 48 hrs, 4-day adult    6 Cadmium 100 mM 48 hrs, 4-day adult    28 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    5 Zinc 5 mM 12 hrs, larvae L3    21 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    49 Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    7 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    0 Caffeine 25 mg/ml 48 hrs, 4-day adult    19 Paraquat 5 mM 48 hrs, 4-day adult    2 Paraquat 10 mM 48 hrs, 4-day adult    2 Rotenone 2 μg 12 hrs, larvae L3    9 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  (56) cold shock, 4-day adult  (49) heat shock, 4-day adult  (48) Cadmium 50 mM 6 hrs, larvae L3    10 Cadmium 50 mM 12 hrs, larvae L3  (56) Cadmium 50 mM 48 hrs, 4-day adult    6 Cadmium 100 mM 48 hrs, 4-day adult  28 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    5 Zinc 5 mM 12 hrs, larvae L3    21 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3  (49) Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    7 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    0 Caffeine 25 mg/ml 48 hrs, 4-day adult    19 Paraquat 5 mM 48 hrs, 4-day adult    2 Paraquat 10 mM 48 hrs, 4-day adult    2 Rotenone 2 μg 12 hrs, larvae L3    9 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    56 cold shock, 4-day adult    49 heat shock, 4-day adult    48 Cadmium 50 mM 6 hrs, larvae L3    10 Cadmium 50 mM 12 hrs, larvae L3    56 Cadmium 50 mM 48 hrs, 4-day adult    6 Cadmium 100 mM 48 hrs, 4-day adult    28 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    5 Zinc 5 mM 12 hrs, larvae L3    21 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    49 Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    7 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    0 Caffeine 25 mg/ml 48 hrs, 4-day adult    19 Paraquat 5 mM 48 hrs, 4-day adult    2 Paraquat 10 mM 48 hrs, 4-day adult    2 Rotenone 2 μg 12 hrs, larvae L3    9 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    56 cold shock, 4-day adult    49 heat shock, 4-day adult    48 Cadmium 50 mM 6 hrs, larvae L3    10 Cadmium 50 mM 12 hrs, larvae L3    56 Cadmium 50 mM 48 hrs, 4-day adult    6 Cadmium 100 mM 48 hrs, 4-day adult    28 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    5 Zinc 5 mM 12 hrs, larvae L3    21 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    49 Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    7 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    0 Caffeine 25 mg/ml 48 hrs, 4-day adult    19 Paraquat 5 mM 48 hrs, 4-day adult    2 Paraquat 10 mM 48 hrs, 4-day adult    2 Rotenone 2 μg 12 hrs, larvae L3    9 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_25
External Data & Images
Linkouts	BDGP expression data • PC00481 IP02523 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0001229 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG4190-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable Hsp67Bcd06009 viable, with Scer\GAL4pnr-MD237 Hsp67BcGD11244 Sterility Allele fertile Hsp67Bcd06009 Phenotype manifest in Allele
Classical Alleles ( 1 )
For All Classical Alleles Show
Allele of Hsp67Bc Class Mutagen Stocks Known lesion Hsp67Bcd06009 P-element activity 2 --
Alleles Carried on Transgenic Constructs ( 6 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Hsp67Bc Class Mutagen Stocks Known lesion Hsp67BcGD11244 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes Hsp67BcGL00377 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Hsp67BcHMS02440 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Hsp67BcKK103547 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Hsp67BcNIG.4190R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes Hsp67BcScer\UAS.T:SV5\V5 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes
Aneuploid Aberrations
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD11244} NA P{KK103547} NA P{NIG.4190R} NA P{TRiP.GL00377} NA P{TRiP.HMS02440} NA P{UAS-Hsp67Bc.V5} NA heat-shock construct P{UAS-Hsp67Bc.V5} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{XP}Hsp67Bcd06009 NA
Gene Ontology: Function, Process & Cellular Component ( 5 unique terms )
Terms Based on Experimental Evidence ( 4 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
protein lipidation inferred from mutant phenotype (Carra et al., 2010) regulation of autophagy inferred from mutant phenotype (Carra et al., 2010) regulation of translational initiation by eIF2 alpha phosphorylation inferred from mutant phenotype (Carra et al., 2010) response to methotrexate inferred from expression pattern (Chahine and O'Donnell, 2010)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 1 term )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
response to heat inferred from sequence or structural similarity with EMBL:U96099 (FlyBase, 1992-) non-traceable author statement (Swiss-Prot Project Members, 1991.8.1)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
Hsp67Bc - Arp1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Hsp67Bc - Arpc2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Hsp67Bc - CG14854 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) Hsp67Bc - GstD7 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Hsp67Bc - Hsp23 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Hsp67Bc allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 64468 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0001229 DroID - A comprehensive database of gene and protein interactions. • FBgn0001229 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 39071
Orthologs
OrthoDB Orthologs (78) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6640ZH Dipteran inclusive ortholog search No orthologs identified Insect inclusive ortholog search EOG6QVB8J Arthropod inclusive ortholog search No orthologs identified Metazoa inclusive ortholog search EOG6FJN62
Orthologs in Drosophila Species (EOG6640ZH)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Hsp67Bc     Drosophila simulans   Dsim\GD12934 Y   Drosophila sechellia   Dsec\GM24882 Y   Drosophila erecta   Dere\GG14048 Y   Drosophila yakuba   Dyak\Hsp67Bc Y   Drosophila ananassae   Dana\GF23740 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA24923 Y   Drosophila persimilis   Dper\GL22633 Y   Drosophila willistoni   Dwil\GK19253 Y   Drosophila virilis   Dvir\GJ13837 Y   Drosophila mojavensis   Dmoj\GI13089 Y   Drosophila grimshawi   Dgri\GH16985 Y
Orthologs in non-Drosophila Dipterans (None identified)
No non-Drosophilid orthologies identified
Orthologs in non-Dipteran Insects (EOG6QVB8J)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Atta cephalotes Leafcutter ant  Acep\ACEP18695 Y Linepithema humile Argentine ant  Lhum\LH25417 Y Pogonomyrmex barbatus Red harvester ant  Pbar\PB27060 Y Pogonomyrmex barbatus Red harvester ant  Pbar\PB11557 Y
Orthologs in non-Insect Arthropods (None identified)
No non-Insect Arthropod orthologies identified
Orthologs in non-Arthropod Metazoa (EOG6FJN62)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\hsp-12.6 Y Caenorhabditis elegans Nematode  Cele\hsp-17 Y Caenorhabditis elegans Nematode  Cele\F08H9.4 Y Caenorhabditis elegans Nematode  Cele\hsp-12.3 Y Caenorhabditis elegans Nematode  Cele\hsp-16.2 Y Caenorhabditis elegans Nematode  Cele\hsp-16.41 Y Caenorhabditis elegans Nematode  Cele\F08H9.3 Y Caenorhabditis elegans Nematode  Cele\hsp-12.1 Y Caenorhabditis elegans Nematode  Cele\C14F11.5 Y Caenorhabditis elegans Nematode  Cele\hsp-12.2 Y Caenorhabditis elegans Nematode  Cele\T27E4.9 Y Caenorhabditis elegans Nematode  Cele\T27E4.3 Y Caenorhabditis elegans Nematode  Cele\sip-1 Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_020294gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_001357gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_018347gn Y Danio rerio Zebrafish  Drer\hspb1 Y Danio rerio Zebrafish  Drer\cryaba Y Danio rerio Zebrafish  Drer\hspb2 Y Danio rerio Zebrafish  Drer\cryaa Y Danio rerio Zebrafish  Drer\cryabb Y Danio rerio Zebrafish  Drer\hspb3 Y Danio rerio Zebrafish  Drer\hspb6 Y Danio rerio Zebrafish  Drer\DKEY-1K23.2 Y Danio rerio Zebrafish  Drer\BX649391.1 Y Danio rerio Zebrafish  Drer\hspb15 Y Xenopus tropicalis Western clawed frog  Xtro\hspb8 Y Xenopus tropicalis Western clawed frog  Xtro\cryaa Y Xenopus tropicalis Western clawed frog  Xtro\hspb1 Y Xenopus tropicalis Western clawed frog  Xtro\ENSXETG00000027465 Y Xenopus tropicalis Western clawed frog  Xtro\hspb2 Y Xenopus tropicalis Western clawed frog  Xtro\hspb6 Y Xenopus tropicalis Western clawed frog  Xtro\hspb3 Y Xenopus tropicalis Western clawed frog  Xtro\cryab Y Gallus gallus Domestic chicken  Ggal\CRYAB Y Gallus gallus Domestic chicken  Ggal\HSPB8 Y Gallus gallus Domestic chicken  Ggal\CRYAA Y Gallus gallus Domestic chicken  Ggal\HSPB1 Y Gallus gallus Domestic chicken  Ggal\HSPB3 Y Gallus gallus Domestic chicken  Ggal\HSPB2 Y Mus musculus House mouse  Mmus\Hspb8 Y Mus musculus House mouse  Mmus\Cryaa Y Mus musculus House mouse  Mmus\Cryab Y Mus musculus House mouse  Mmus\Hspb1 Y Mus musculus House mouse  Mmus\Hspb2 Y Mus musculus House mouse  Mmus\Hspb6 Y Mus musculus House mouse  Mmus\Hspb3 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000007015 Y Rattus norvegicus Norway rat  Rnor\Hspb3 Y Rattus norvegicus Norway rat  Rnor\Hspb8 Y Rattus norvegicus Norway rat  Rnor\Hspb6 Y Rattus norvegicus Norway rat  Rnor\Cryab Y Rattus norvegicus Norway rat  Rnor\Hspb1 Y Rattus norvegicus Norway rat  Rnor\Hspb2 Y Homo sapiens Human  Hsap\HSPB6 Y Homo sapiens Human  Hsap\HSPB2 Y Homo sapiens Human  Hsap\HSPB2 Y Homo sapiens Human  Hsap\HSPB3 Y Homo sapiens Human  Hsap\HSPB1 Y Homo sapiens Human  Hsap\HSPB8 Y Homo sapiens Human  Hsap\CRYAB Y Homo sapiens Human  Hsap\CRYAA Y
Human Orthologs (8)
Gene OMIM HGNC Hsap\HSPB6     Hsap\HSPB2     Hsap\HSPB2     Hsap\HSPB3     Hsap\HSPB1     Hsap\HSPB8     Hsap\CRYAB     Hsap\CRYAA
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD12934 Drosophila sechellia Dsec\GM24882 Drosophila erecta Dere\GG14048 Drosophila yakuba Dyak\Hsp67Bc Drosophila ananassae Dana\GF23740 Drosophila pseudoobscura pseudoobscura Dpse\GA24923 Drosophila persimilis Dper\GL22633 Drosophila willistoni Dwil\GK19253 Drosophila virilis Dvir\GJ13837 Drosophila mojavensis Dmoj\GI13089 Drosophila grimshawi Dgri\GH16985
Stocks & Reagents
Stocks Listed in FlyBase ( 7 )
Bloomington	19227 w1118; P{XP}Hsp67Bcd06009 42607 y1 sc* v1; P{TRiP.HMS02440}attP40 35452 y1 sc* v1; P{TRiP.GL00377}attP2/TM3, Sb1
Harvard	- P{XP}Hsp67Bcd06009
VDRC	v103974 P{KK103547}VIE-260B v26416 w1118; P{GD11244}v26416 v26417 w1118; P{GD11244}v26417
Genomic Clones ( 1 )
	BACR01E20 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 26 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	IP02523
Other clones	DPiM_IP02523 yak-em_Hsp67Bc
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones
Other clones	53075 101768 145189 CK01.58B.A8 EC25987 EC29101 EC37611 EK046936 EK170061 EK228512 ESG013b.E21_B01 ESG016c.E21_B08 ESG017b.E21_B02 ESG018c.E21.1_D04 ESG0110c.E21.1_C07 ESG0116b.B21_G01 ESG0118a.B21_D07 FGC010A08 FGM207H05 IP02521 IP02522 IP02524 RP004546748
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0001229 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 39071
Antibody Information
	polyclonal (Carra et al., 2010)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments
Other Comments
	In unshocked cells Hsp83 is moderately transcribed while transcription from the other heat shock genes is undetectable. Engaged but paused RNA molecules are found at the various Hsp70 and Hsp26 genes but not at the other heat shock genes. Increased transcription of the heat shock genes is observed within 1-2 mins of heat shock and maximal rates were reached within 2-5 minutes. Rates of transcription vary over a 20-fold range. (Vazquez et al., 1993) Exposure of cells to pulses of elevated temperature initiates the heat-shock response. A restricted subset of genes, the Hsp genes, is activated and the majority of transcription and translation is shut down. 3H-uridine incorporation ceases at its usual positions and commences at new puff sites. Preexisting polysomes disaggregate and within a few minutes a new population of polysomes appears containing newly transcribed mRNA; this RNA hybridizes to some of the heat-shock puffs. Similar response inducible by other stressful treatments. The response may be elicited at all stages of the life cycle and in cultured cells. (Lindsley and Zimm, 1992) Activation of transcription of Hsp genes apparently involves the sequential binding of two or more protein factors in vicinity of TATA box. (Wu, 1984) Polymerase II dissociates from most chromosome regions and accumulates at the new heat shock puff sites upon heat shock. (Bonner and Kerby, 1982) The binding sites for the protein factors required for activation of transcription of Hsp genes are multiple short upstream sequence elements called HSEs or heat shock consensus elements. (Pelham, 1982, Xiao and Lis, 1988) Hsp67Bc is transcribed during certain developmental stages in the absence of heat shock. (Sirotkin and Davidson, 1982) The effects of heat shock may be abrogated to some degree by pretreatment with a pulse of a slightly lower temperature. (Mitchell et al., 1979, Petersen and Mitchell, 1981) Mitochondrial and histone-gene activities persist transcription and translation. (Spradling et al., 1977) In polytene cells, during heat shock response, existing puffs regress and a novel group quickly appear at 33B, 63C, 64F, 67B, 70A, 87A, 87C, 93D, 95D. (Tissieres et al., 1974) The heat shock response follows a pulse of 36oC to 40oC; treatments above 40oC inhibit all activity and lead to death; treatments of 30oC-35oC induce heat-shock-protein synthesis without repressing normal protein synthesis. (Tissieres et al., 1974) In polytene cells, during heat shock response, existing puffs regress and a novel group quickly appear at cytological locations 33B, 63C, 64F, 67B, 70A, 87A, 87C, 93D, 95D. (Ashburner, 1970)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_079270 RefSeq (Proteins) • NP_523994 DDBJ / EMBL / GenBank • AAF50292 CAA29788 X06542 Entrez Gene - A searchable database of RefSeq genes. • 39071 UniProtKB/Swiss-Prot • P22979
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Alpha crystallin/Heat shock protein (IPR001436) Alpha crystallin/Hsp20 domain (IPR002068) HSP20-like chaperone (IPR008978) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • IP02523 PC00481
Linkouts
	BioGRID - A database of protein and genetic interactions • 64468 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0001229 DroID - A comprehensive database of gene and protein interactions. • FBgn0001229 DRSC - Results from RNAi screens. • FBgn0001229 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0001229 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG4190-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0001229 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 39071 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 39071 modMine - Data generated by the modENCODE project. • FBgn0001229
Synonyms & Secondary IDs ( 18 )
Reported As
Symbol Synonym	CG4190 (Whitfield, 2001.1.5, Keleman et al., 2009.8.5, Carra et al., 2010, Chahine and O'Donnell, 2010, Ni et al., 2011.7.19, Ni et al., 2010.12.1) Dm-HSP67Bc (Carra et al., 2010) gene3 (Southgate et al., 1985) gene 3 (Vazquez et al., 1993, Vazquez, 1991, Pauli et al., 1989, Pauli et al., 1988, Ayme-Southgate and Tissieres, 1985) gene-3   gene5 (Sirotkin and Davidson, 1982) HSP67 (Chahine and O'Donnell, 2010) hsp67B (Ringrose et al., 2003) Hsp67Bc (Sudi et al., 2008, Harvey et al., 2008, Bettencourt et al., 2008, Birch-Machin et al., 2005, Xi et al., 2008, Takahashi et al., 2010, Carra et al., 2010, Rehwinkel et al., 2004, Japanese National Institute of Genetics, 2012.5.21) HSP67Bc (Zhang et al., 2011, Carra et al., 2010, Chahine and O'Donnell, 2010) Hsp67BC (Mosqueira et al., 2010) HSPB8 (Carra et al., 2010) Hspg3 (Maroni, 1994) Hsp-G3   small hsp locus 67B (Yao et al., 2007) unnamed (Pauli, 1999.9.9)
Name Synonym	Gene 3 (Pauli and Tonka, 1987) Heat shock gene 67Bc
Secondary FlyBase IDs
References ( 59 )
Generate a list of	All references relating to this gene ( 59 )
List References by type	Research paper  ( 38 ) Supplementary material  ( 4 ) Personal communication to FlyBase  ( 8 ) FlyBase analysis  ( 1 ) DNA/RNA sequence record  ( 1 ) Stock list  ( 1 ) Book  ( 1 ) Protein sequence record  ( 1 ) Website  ( 1 ) Curated genome annotation  ( 2 )
Recent research papers ( 3 )
	Baradaran-Heravi et al., 2012, Hum. Mol. Genet. 21(11): 2572--2587 Penetrance of biallelic SMARCAL1 mutations is associated with environmental and genetic disturbances of gene expression. [FBrf0218299] Vesala et al., 2012, Insect Mol. Biol. 21(1): 107--118 Cold tolerance and cold-induced modulation of gene expression in two Drosophila virilis group species with different distributions. [FBrf0217233] Zhang et al., 2011, J. Molec. Cell. Cardiol. 51(3): 381--389 Effects of different small HSPB members on contractile dysfunction and structural changes in a Drosophila melanogaster model for Atrial Fibrillation. [FBrf0214619] Show all research papers ...