Gene Dmel\if

Experimentally Determined Recombination Data

Location

1-55

(Curry, 1939)

Left of (cM)

(Curry, 1939)

Right of (cM)

(Wilcox et al., 1989, Green, 1997.8.18)

(Curry, 1939)

Notes

Gene Model & Products

Please see the GBrowse view of Dmel\if for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

if-RB

FBtr0074357

NM_078654

5724

1396

if-RC

FBtr0301352

NM_167544

5649

1371

if-RD

FBtr0301353

NM_001169306

5633

1396

if-RF

FBtr0301354

NM_001169307

5818

1396

Additional Transcript Data & Comments

Reported size (kB)

5.7 (northern blot)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

if-PB

FBpp0074131

154.3

1396

6.10

NP_523378

AAF48661

if-PC

FBpp0290566

151.6

1371

6.04

NP_728021

AAN09423

if-PD

FBpp0290567

154.3

1396

6.10

NP_001162777

ACZ95312

if-PF

FBpp0290568

154.3

1396

6.10

NP_001162778

ACZ95313

Additional Polypeptide Data & Comments

Reported size (kDa)

1394 (aa); 140 (kD observed)

Comments

External Data

FBsf0000436166
X:16,675,998..16,676,371

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Integrin alpha chain, C-terminal cytoplasmic region, conserved site (IPR018184)

Integrin alpha chain (IPR000413)

Integrin alpha beta-propellor (IPR013519)

Integrin alpha-2 (IPR013649)

FG-GAP repeat (IPR013517)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

P12080

UniProtKB/TrEMBL

Mapped Features

Mapped Features have been reorganized, please see this article for details.

Additional mapped features and mutations can be found on GBrowse or related reports.

Type

Symbol & Location

Additional Notes

References

regulatory region

evidence=Reporter construct (in vivo)

(Junion et al., 2005, Halfon, 2012.6.19)

External Data

Crossreferences

Expression Data

Transcript Expression

radioisotope in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 4 -- 13

mesoderm

third instar larval stage

peripodial epithelium

wing disc

Additional Descriptive Data

if transcripts are expressed throughout development and in adults. In embryos, they are first detected at stage 4 in the presumptive mesoderm. They continue to be expressed in mesodermal cells after invagination. In third instar larval wing discs, if transcripts are primarily restricted to the ventral compartment and to the peripodial membrane.

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

gonadal sheath muscle

visceral mesoderm

muscle attachment site

pharyngeal muscle

gonad | restricted

embryonic stage 10 -- 17

mesoderm

embryonic stage 16

muscle attachment site

third instar larval stage

wing disc

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

Additional Descriptive Data

if protein is first detected in stage 10 embryos in the mesoderm where it is basally concentrated in cuboidal cells which lie on the ectoderm. After the somatic and visceral mesoderm separates, if protein is found on the basal cell surfaces of the visceral myoblasts when they attach to the foregut, midgut, and hindgut. It is also found at the sites of attachment of the somatic muscles. Protein was also detected in the gonadal sheath and in the interstitial cells of the gonad In third instar larval wing discs, if protein is primarily restricted to the ventral compartment.

Marker for

Subcellular Localization (GO Cellular Component)

CV term

(Tanentzapf et al., 2006)

References

plasma membrane

inferred from direct assay

sarcolemma

inferred from direct assay

(The modENCODE Consortium, 2010, Graveley et al., 2011)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

Reference

See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.

FlyAtlas Anatomy Microarray

FlyAtlas Anatomical Expression Data

(FlyAtlas-RNA.adult)

(FlyAtlas-RNA.larva)

	Styles
	Linear
	Logarithmic
	Heatmap
	Back-to-back
	Scales
	gene max expression
	Moderate expression bin max
	High level expression bin max
	Very high expression bin max

Summary of FlyAtlas Anatomical Expression Data: Many larval and adult organs/tissues expressed at moderate or high levels. Expression at high levels in the following post-embryonic organs or tissues: adult crop, larval/adult hindgut, larval Malpighian tubules, larval carcass. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, larval/adult central nervous system, adult Malpighian tubules, adult heart, adult fat body, larval/adult salivary gland, larval trachea, adult spermathecae, adult carcass.
[download data (TSV)]

Guide to FlyAtlas expression level colors
	No expression (0 - 9.999)
	Low expression (10 - 99.999)
	Moderate expression (100 - 499.999)
	High level expression (500 - 999.999)
	Very high expression (>999.999)

Linear, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		145.2
Larval Midgut		85.8
Larval Hindgut		554.4
Larval Malpighian Tubules		1185.7
Larval Fat Body		23.4
Larval Salivary Gland		493.2
Larval Trachea		349.45
Larval Carcass		991.75
Adult Head		204.7
Adult Eye		68.375
Adult Brain		78.5
Adult Thoracic-Abdominal Ganglion		117.7
Adult Crop		925.1
Adult Midgut		87.8
Adult Hindgut		579.7
Adult Malpighian Tubules		300.7
Adult Fat Body		160.3
Adult Salivary Gland		183.6
Adult Heart		255.675
Adult VirginFemale Spermatheca		234.7
Adult InseminatedFemale Spermatheca		193.6
Adult Ovary		21.5
Adult Testis		34.5
Adult Male Accessory Gland		96.7
Adult Carcass		280.3
Expression Level Scale		None Low Moderate High Very high

Linear, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		145.2
Larval Midgut		85.8
Larval Hindgut		(554.4)
Larval Malpighian Tubules		(1185.7)
Larval Fat Body		23.4
Larval Salivary Gland		493.2
Larval Trachea		349.45
Larval Carcass		(991.75)
Adult Head		204.7
Adult Eye		68.375
Adult Brain		78.5
Adult Thoracic-Abdominal Ganglion		117.7
Adult Crop		(925.1)
Adult Midgut		87.8
Adult Hindgut		(579.7)
Adult Malpighian Tubules		300.7
Adult Fat Body		160.3
Adult Salivary Gland		183.6
Adult Heart		255.675
Adult VirginFemale Spermatheca		234.7
Adult InseminatedFemale Spermatheca		193.6
Adult Ovary		21.5
Adult Testis		34.5
Adult Male Accessory Gland		96.7
Adult Carcass		280.3
Expression Level Scale		None Low Moderate High

Linear, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		145.2
Larval Midgut		85.8
Larval Hindgut		554.4
Larval Malpighian Tubules		(1185.7)
Larval Fat Body		23.4
Larval Salivary Gland		493.2
Larval Trachea		349.45
Larval Carcass		991.75
Adult Head		204.7
Adult Eye		68.375
Adult Brain		78.5
Adult Thoracic-Abdominal Ganglion		117.7
Adult Crop		925.1
Adult Midgut		87.8
Adult Hindgut		579.7
Adult Malpighian Tubules		300.7
Adult Fat Body		160.3
Adult Salivary Gland		183.6
Adult Heart		255.675
Adult VirginFemale Spermatheca		234.7
Adult InseminatedFemale Spermatheca		193.6
Adult Ovary		21.5
Adult Testis		34.5
Adult Male Accessory Gland		96.7
Adult Carcass		280.3
Expression Level Scale		None Low Moderate High Very high

Linear, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		145.2
Larval Midgut		85.8
Larval Hindgut		554.4
Larval Malpighian Tubules		1185.7
Larval Fat Body		23.4
Larval Salivary Gland		493.2
Larval Trachea		349.45
Larval Carcass		991.75
Adult Head		204.7
Adult Eye		68.375
Adult Brain		78.5
Adult Thoracic-Abdominal Ganglion		117.7
Adult Crop		925.1
Adult Midgut		87.8
Adult Hindgut		579.7
Adult Malpighian Tubules		300.7
Adult Fat Body		160.3
Adult Salivary Gland		183.6
Adult Heart		255.675
Adult VirginFemale Spermatheca		234.7
Adult InseminatedFemale Spermatheca		193.6
Adult Ovary		21.5
Adult Testis		34.5
Adult Male Accessory Gland		96.7
Adult Carcass		280.3
Expression Level Scale		Very high

log, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		145.2
Larval Midgut		85.8
Larval Hindgut		554.4
Larval Malpighian Tubules		1185.7
Larval Fat Body		23.4
Larval Salivary Gland		493.2
Larval Trachea		349.45
Larval Carcass		991.75
Adult Head		204.7
Adult Eye		68.375
Adult Brain		78.5
Adult Thoracic-Abdominal Ganglion		117.7
Adult Crop		925.1
Adult Midgut		87.8
Adult Hindgut		579.7
Adult Malpighian Tubules		300.7
Adult Fat Body		160.3
Adult Salivary Gland		183.6
Adult Heart		255.675
Adult VirginFemale Spermatheca		234.7
Adult InseminatedFemale Spermatheca		193.6
Adult Ovary		21.5
Adult Testis		34.5
Adult Male Accessory Gland		96.7
Adult Carcass		280.3
Expression Level Scale		None Low Moderate High Very high

log, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		145.2
Larval Midgut		85.8
Larval Hindgut		554.4
Larval Malpighian Tubules		(1185.7)
Larval Fat Body		23.4
Larval Salivary Gland		493.2
Larval Trachea		349.45
Larval Carcass		(991.75)
Adult Head		204.7
Adult Eye		68.375
Adult Brain		78.5
Adult Thoracic-Abdominal Ganglion		117.7
Adult Crop		(925.1)
Adult Midgut		87.8
Adult Hindgut		579.7
Adult Malpighian Tubules		300.7
Adult Fat Body		160.3
Adult Salivary Gland		183.6
Adult Heart		255.675
Adult VirginFemale Spermatheca		234.7
Adult InseminatedFemale Spermatheca		193.6
Adult Ovary		21.5
Adult Testis		34.5
Adult Male Accessory Gland		96.7
Adult Carcass		280.3
Expression Level Scale		None Low Moderate High

log, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		145.2
Larval Midgut		85.8
Larval Hindgut		554.4
Larval Malpighian Tubules		1185.7
Larval Fat Body		23.4
Larval Salivary Gland		493.2
Larval Trachea		349.45
Larval Carcass		991.75
Adult Head		204.7
Adult Eye		68.375
Adult Brain		78.5
Adult Thoracic-Abdominal Ganglion		117.7
Adult Crop		925.1
Adult Midgut		87.8
Adult Hindgut		579.7
Adult Malpighian Tubules		300.7
Adult Fat Body		160.3
Adult Salivary Gland		183.6
Adult Heart		255.675
Adult VirginFemale Spermatheca		234.7
Adult InseminatedFemale Spermatheca		193.6
Adult Ovary		21.5
Adult Testis		34.5
Adult Male Accessory Gland		96.7
Adult Carcass		280.3
Expression Level Scale		None Low Moderate High Very high

log, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		145.2
Larval Midgut		85.8
Larval Hindgut		554.4
Larval Malpighian Tubules		1185.7
Larval Fat Body		23.4
Larval Salivary Gland		493.2
Larval Trachea		349.45
Larval Carcass		991.75
Adult Head		204.7
Adult Eye		68.375
Adult Brain		78.5
Adult Thoracic-Abdominal Ganglion		117.7
Adult Crop		925.1
Adult Midgut		87.8
Adult Hindgut		579.7
Adult Malpighian Tubules		300.7
Adult Fat Body		160.3
Adult Salivary Gland		183.6
Adult Heart		255.675
Adult VirginFemale Spermatheca		234.7
Adult InseminatedFemale Spermatheca		193.6
Adult Ovary		21.5
Adult Testis		34.5
Adult Male Accessory Gland		96.7
Adult Carcass		280.3
Expression Level Scale		None Low Moderate High Very high

Heatmap
Tissue		Expression Level
Larval Central Nervous System
Larval Midgut
Larval Hindgut
Larval Malpighian Tubules
Larval Fat Body
Larval Salivary Gland
Larval Trachea
Larval Carcass
Adult Head
Adult Eye
Adult Brain
Adult Thoracic-Abdominal Ganglion
Adult Crop
Adult Midgut
Adult Hindgut
Adult Malpighian Tubules
Adult Fat Body
Adult Salivary Gland
Adult Heart
Adult VirginFemale Spermatheca
Adult InseminatedFemale Spermatheca
Adult Ovary
Adult Testis
Adult Male Accessory Gland
Adult Carcass

FlyAtlas Organ/Tissue Expression, larval vs. adult
Larval Expression Level	Tissue	Adult Expression Level
NA	Head	204.7
NA	Eye	68.375
NA	Brain	78.5
145.2	Central Nervous System	NA
NA	Thoracic-Abdominal Ganglion	117.7
NA	Crop	925.1
85.8	Midgut	87.8
554.4	Hindgut	579.7
1185.7	Malpighian Tubules	300.7
no informative data	Fat Body	160.3
493.2	Salivary Gland	183.6
NA	Heart	255.675
349.45	Trachea	NA
NA	VirginFemale Spermatheca	234.7
NA	InseminatedFemale Spermatheca	193.6
NA	Ovary	21.5
NA	Testis	34.5
NA	Male Accessory Gland	96.7
991.75	Carcass	280.3

FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)

modENCODE Anatomy RNA-Seq

modENCODE Tissue Expression Data

(modENCODE_mRNA-Seq_tissues)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		7
central nervous system, larvae L3		5
central nervous system, pupae P8		5
head, virgin 1-day female		14
head, virgin 4-day female		9
head, virgin 20-day female		11
head, mated 1-day female		10
head, mated 4-day female		9
head, mated 20-day female		8
head, mated 1-day male		15
head, mated 4-day male		12
head, mated 20-day male		14
salivary gland, larvae L3 wandering		1
salivary gland, white prepupae		1
digestive system, larvae L3 wandering		4
digestive system, 1-day adult		6
digestive system, 4-day adult		6
digestive system, 20-day adult		5
fat body, larvae L3 wandering		3
fat body, white prepupae		2
fat body, pupae P8		4
carcass, larvae L3 wandering		14
carcass, 1-day adult		22
carcass, 4-day adult		17
carcass, 20-day adult		15
ovary, virgin 4-day female		1
ovary, mated 4-day female		1
testis, mated 4-day male		1
accessory gland, mated 4-day male		2
Expression Level Scale		Very low Low Moderate

Linear, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		7
central nervous system, larvae L3		5
central nervous system, pupae P8		5
head, virgin 1-day female		14
head, virgin 4-day female		9
head, virgin 20-day female		11
head, mated 1-day female		10
head, mated 4-day female		9
head, mated 20-day female		8
head, mated 1-day male		15
head, mated 4-day male		12
head, mated 20-day male		14
salivary gland, larvae L3 wandering		1
salivary gland, white prepupae		1
digestive system, larvae L3 wandering		4
digestive system, 1-day adult		6
digestive system, 4-day adult		6
digestive system, 20-day adult		5
fat body, larvae L3 wandering		3
fat body, white prepupae		2
fat body, pupae P8		4
carcass, larvae L3 wandering		14
carcass, 1-day adult		22
carcass, 4-day adult		17
carcass, 20-day adult		15
ovary, virgin 4-day female		1
ovary, mated 4-day female		1
testis, mated 4-day male		1
accessory gland, mated 4-day male		2
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		7
central nervous system, larvae L3		5
central nervous system, pupae P8		5
head, virgin 1-day female		14
head, virgin 4-day female		9
head, virgin 20-day female		11
head, mated 1-day female		10
head, mated 4-day female		9
head, mated 20-day female		8
head, mated 1-day male		15
head, mated 4-day male		12
head, mated 20-day male		14
salivary gland, larvae L3 wandering		1
salivary gland, white prepupae		1
digestive system, larvae L3 wandering		4
digestive system, 1-day adult		6
digestive system, 4-day adult		6
digestive system, 20-day adult		5
fat body, larvae L3 wandering		3
fat body, white prepupae		2
fat body, pupae P8		4
carcass, larvae L3 wandering		14
carcass, 1-day adult		22
carcass, 4-day adult		17
carcass, 20-day adult		15
ovary, virgin 4-day female		1
ovary, mated 4-day female		1
testis, mated 4-day male		1
accessory gland, mated 4-day male		2
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		7
central nervous system, larvae L3		5
central nervous system, pupae P8		5
head, virgin 1-day female		14
head, virgin 4-day female		9
head, virgin 20-day female		11
head, mated 1-day female		10
head, mated 4-day female		9
head, mated 20-day female		8
head, mated 1-day male		15
head, mated 4-day male		12
head, mated 20-day male		14
salivary gland, larvae L3 wandering		1
salivary gland, white prepupae		1
digestive system, larvae L3 wandering		4
digestive system, 1-day adult		6
digestive system, 4-day adult		6
digestive system, 20-day adult		5
fat body, larvae L3 wandering		3
fat body, white prepupae		2
fat body, pupae P8		4
carcass, larvae L3 wandering		14
carcass, 1-day adult		22
carcass, 4-day adult		17
carcass, 20-day adult		15
ovary, virgin 4-day female		1
ovary, mated 4-day female		1
testis, mated 4-day male		1
accessory gland, mated 4-day male		2
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		7
central nervous system, larvae L3		5
central nervous system, pupae P8		5
head, virgin 1-day female		14
head, virgin 4-day female		9
head, virgin 20-day female		11
head, mated 1-day female		10
head, mated 4-day female		9
head, mated 20-day female		8
head, mated 1-day male		15
head, mated 4-day male		12
head, mated 20-day male		14
salivary gland, larvae L3 wandering		1
salivary gland, white prepupae		1
digestive system, larvae L3 wandering		4
digestive system, 1-day adult		6
digestive system, 4-day adult		6
digestive system, 20-day adult		5
fat body, larvae L3 wandering		3
fat body, white prepupae		2
fat body, pupae P8		4
carcass, larvae L3 wandering		14
carcass, 1-day adult		22
carcass, 4-day adult		17
carcass, 20-day adult		15
ovary, virgin 4-day female		1
ovary, mated 4-day female		1
testis, mated 4-day male		1
accessory gland, mated 4-day male		2
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		7
central nervous system, larvae L3		5
central nervous system, pupae P8		5
head, virgin 1-day female		14
head, virgin 4-day female		9
head, virgin 20-day female		11
head, mated 1-day female		10
head, mated 4-day female		9
head, mated 20-day female		8
head, mated 1-day male		15
head, mated 4-day male		12
head, mated 20-day male		14
salivary gland, larvae L3 wandering		1
salivary gland, white prepupae		1
digestive system, larvae L3 wandering		4
digestive system, 1-day adult		6
digestive system, 4-day adult		6
digestive system, 20-day adult		5
fat body, larvae L3 wandering		3
fat body, white prepupae		2
fat body, pupae P8		4
carcass, larvae L3 wandering		14
carcass, 1-day adult		22
carcass, 4-day adult		17
carcass, 20-day adult		15
ovary, virgin 4-day female		1
ovary, mated 4-day female		1
testis, mated 4-day male		1
accessory gland, mated 4-day male		2
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		7
central nervous system, larvae L3		5
central nervous system, pupae P8		5
head, virgin 1-day female		14
head, virgin 4-day female		9
head, virgin 20-day female		11
head, mated 1-day female		10
head, mated 4-day female		9
head, mated 20-day female		8
head, mated 1-day male		15
head, mated 4-day male		12
head, mated 20-day male		14
salivary gland, larvae L3 wandering		1
salivary gland, white prepupae		1
digestive system, larvae L3 wandering		4
digestive system, 1-day adult		6
digestive system, 4-day adult		6
digestive system, 20-day adult		5
fat body, larvae L3 wandering		3
fat body, white prepupae		2
fat body, pupae P8		4
carcass, larvae L3 wandering		14
carcass, 1-day adult		22
carcass, 4-day adult		17
carcass, 20-day adult		15
ovary, virgin 4-day female		1
ovary, mated 4-day female		1
testis, mated 4-day male		1
accessory gland, mated 4-day male		2
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		7
central nervous system, larvae L3		5
central nervous system, pupae P8		5
head, virgin 1-day female		14
head, virgin 4-day female		9
head, virgin 20-day female		11
head, mated 1-day female		10
head, mated 4-day female		9
head, mated 20-day female		8
head, mated 1-day male		15
head, mated 4-day male		12
head, mated 20-day male		14
salivary gland, larvae L3 wandering		1
salivary gland, white prepupae		1
digestive system, larvae L3 wandering		4
digestive system, 1-day adult		6
digestive system, 4-day adult		6
digestive system, 20-day adult		5
fat body, larvae L3 wandering		3
fat body, white prepupae		2
fat body, pupae P8		4
carcass, larvae L3 wandering		14
carcass, 1-day adult		22
carcass, 4-day adult		17
carcass, 20-day adult		15
ovary, virgin 4-day female		1
ovary, mated 4-day female		1
testis, mated 4-day male		1
accessory gland, mated 4-day male		2
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Tissue		Expression Level
imaginal disc, larvae L3 wandering
central nervous system, larvae L3
central nervous system, pupae P8
head, virgin 1-day female
head, virgin 4-day female
head, virgin 20-day female
head, mated 1-day female
head, mated 4-day female
head, mated 20-day female
head, mated 1-day male
head, mated 4-day male
head, mated 20-day male
salivary gland, larvae L3 wandering
salivary gland, white prepupae
digestive system, larvae L3 wandering
digestive system, 1-day adult
digestive system, 4-day adult
digestive system, 20-day adult
fat body, larvae L3 wandering
fat body, white prepupae
fat body, pupae P8
carcass, larvae L3 wandering
carcass, 1-day adult
carcass, 4-day adult
carcass, 20-day adult
ovary, virgin 4-day female
ovary, mated 4-day female
testis, mated 4-day male
accessory gland, mated 4-day male

modENCODE Development RNA-Seq

modENCODE Temporal Expression Data

(modENCODE_mRNA-Seq_U)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of very low expression. Peak expression observed within 12-24 hour embryonic stages.
[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		1
embryo 02-04hr		6
embryo 04-06hr		12
embryo 06-08hr		13
embryo 08-10hr		12
embryo 10-12hr		18
embryo 12-14hr		25
embryo 14-16hr		46
embryo 16-18hr		71
embryo 18-20hr		118
embryo 20-22hr		63
embryo 22-24hr		61
larva L1		29
larva L2		23
larva L3 12hr old		33
larva L3 puffstage 1-2		15
larva L3 puffstage 3-6		19
larva L3 puffstage 7-9		16
white prepupae new		13
white prepupae 12hr		14
white prepupae 24hr		13
pupae 2d postWPP		17
pupae 3d postWPP		50
pupae 4d postWPP		36
adult male 01day		28
adult male 05day		24
adult male 30day		22
adult female 01day		17
adult female 05day		6
adult female 30day		7
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		1
embryo 02-04hr		6
embryo 04-06hr		12
embryo 06-08hr		13
embryo 08-10hr		12
embryo 10-12hr		18
embryo 12-14hr		25
embryo 14-16hr		(46)
embryo 16-18hr		(71)
embryo 18-20hr		(118)
embryo 20-22hr		(63)
embryo 22-24hr		(61)
larva L1		(29)
larva L2		23
larva L3 12hr old		(33)
larva L3 puffstage 1-2		15
larva L3 puffstage 3-6		19
larva L3 puffstage 7-9		16
white prepupae new		13
white prepupae 12hr		14
white prepupae 24hr		13
pupae 2d postWPP		17
pupae 3d postWPP		(50)
pupae 4d postWPP		(36)
adult male 01day		(28)
adult male 05day		24
adult male 30day		22
adult female 01day		17
adult female 05day		6
adult female 30day		7
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		1
embryo 02-04hr		6
embryo 04-06hr		12
embryo 06-08hr		13
embryo 08-10hr		12
embryo 10-12hr		18
embryo 12-14hr		25
embryo 14-16hr		46
embryo 16-18hr		71
embryo 18-20hr		(118)
embryo 20-22hr		63
embryo 22-24hr		61
larva L1		29
larva L2		23
larva L3 12hr old		33
larva L3 puffstage 1-2		15
larva L3 puffstage 3-6		19
larva L3 puffstage 7-9		16
white prepupae new		13
white prepupae 12hr		14
white prepupae 24hr		13
pupae 2d postWPP		17
pupae 3d postWPP		50
pupae 4d postWPP		36
adult male 01day		28
adult male 05day		24
adult male 30day		22
adult female 01day		17
adult female 05day		6
adult female 30day		7
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		1
embryo 02-04hr		6
embryo 04-06hr		12
embryo 06-08hr		13
embryo 08-10hr		12
embryo 10-12hr		18
embryo 12-14hr		25
embryo 14-16hr		46
embryo 16-18hr		71
embryo 18-20hr		118
embryo 20-22hr		63
embryo 22-24hr		61
larva L1		29
larva L2		23
larva L3 12hr old		33
larva L3 puffstage 1-2		15
larva L3 puffstage 3-6		19
larva L3 puffstage 7-9		16
white prepupae new		13
white prepupae 12hr		14
white prepupae 24hr		13
pupae 2d postWPP		17
pupae 3d postWPP		50
pupae 4d postWPP		36
adult male 01day		28
adult male 05day		24
adult male 30day		22
adult female 01day		17
adult female 05day		6
adult female 30day		7
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		1
embryo 02-04hr		6
embryo 04-06hr		12
embryo 06-08hr		13
embryo 08-10hr		12
embryo 10-12hr		18
embryo 12-14hr		25
embryo 14-16hr		46
embryo 16-18hr		71
embryo 18-20hr		118
embryo 20-22hr		63
embryo 22-24hr		61
larva L1		29
larva L2		23
larva L3 12hr old		33
larva L3 puffstage 1-2		15
larva L3 puffstage 3-6		19
larva L3 puffstage 7-9		16
white prepupae new		13
white prepupae 12hr		14
white prepupae 24hr		13
pupae 2d postWPP		17
pupae 3d postWPP		50
pupae 4d postWPP		36
adult male 01day		28
adult male 05day		24
adult male 30day		22
adult female 01day		17
adult female 05day		6
adult female 30day		7
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		1
embryo 02-04hr		6
embryo 04-06hr		12
embryo 06-08hr		13
embryo 08-10hr		12
embryo 10-12hr		18
embryo 12-14hr		25
embryo 14-16hr		(46)
embryo 16-18hr		(71)
embryo 18-20hr		(118)
embryo 20-22hr		(63)
embryo 22-24hr		(61)
larva L1		29
larva L2		23
larva L3 12hr old		(33)
larva L3 puffstage 1-2		15
larva L3 puffstage 3-6		19
larva L3 puffstage 7-9		16
white prepupae new		13
white prepupae 12hr		14
white prepupae 24hr		13
pupae 2d postWPP		17
pupae 3d postWPP		(50)
pupae 4d postWPP		(36)
adult male 01day		28
adult male 05day		24
adult male 30day		22
adult female 01day		17
adult female 05day		6
adult female 30day		7
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		1
embryo 02-04hr		6
embryo 04-06hr		12
embryo 06-08hr		13
embryo 08-10hr		12
embryo 10-12hr		18
embryo 12-14hr		25
embryo 14-16hr		46
embryo 16-18hr		71
embryo 18-20hr		118
embryo 20-22hr		63
embryo 22-24hr		61
larva L1		29
larva L2		23
larva L3 12hr old		33
larva L3 puffstage 1-2		15
larva L3 puffstage 3-6		19
larva L3 puffstage 7-9		16
white prepupae new		13
white prepupae 12hr		14
white prepupae 24hr		13
pupae 2d postWPP		17
pupae 3d postWPP		50
pupae 4d postWPP		36
adult male 01day		28
adult male 05day		24
adult male 30day		22
adult female 01day		17
adult female 05day		6
adult female 30day		7
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		1
embryo 02-04hr		6
embryo 04-06hr		12
embryo 06-08hr		13
embryo 08-10hr		12
embryo 10-12hr		18
embryo 12-14hr		25
embryo 14-16hr		46
embryo 16-18hr		71
embryo 18-20hr		118
embryo 20-22hr		63
embryo 22-24hr		61
larva L1		29
larva L2		23
larva L3 12hr old		33
larva L3 puffstage 1-2		15
larva L3 puffstage 3-6		19
larva L3 puffstage 7-9		16
white prepupae new		13
white prepupae 12hr		14
white prepupae 24hr		13
pupae 2d postWPP		17
pupae 3d postWPP		50
pupae 4d postWPP		36
adult male 01day		28
adult male 05day		24
adult male 30day		22
adult female 01day		17
adult female 05day		6
adult female 30day		7
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Developmental Stage		Expression Level
embryo 00-02hr
embryo 02-04hr
embryo 04-06hr
embryo 06-08hr
embryo 08-10hr
embryo 10-12hr
embryo 12-14hr
embryo 14-16hr
embryo 16-18hr
embryo 18-20hr
embryo 20-22hr
embryo 22-24hr
larva L1
larva L2
larva L3 12hr old
larva L3 puffstage 1-2
larva L3 puffstage 3-6
larva L3 puffstage 7-9
white prepupae new
white prepupae 12hr
white prepupae 24hr
pupae 2d postWPP
pupae 3d postWPP
pupae 4d postWPP
adult male 01day
adult male 05day
adult male 30day
adult female 01day
adult female 05day
adult female 30day

modENCODE Temporal Expression Data (Graveley et al., 2011)

modENCODE Cell Lines RNA-Seq

modENCODE Cell Line Expression Data

(modENCODE_mRNA-Seq_cell.A)

(modENCODE_mRNA-Seq_cell.B)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		2
Schneider line 2 Sg4		39
embryonic 1182-4H		1
embryonic GM2		0
embryonic Kc167		0
embryonic S1		0
embryonic S3		41
leg disc CME L1		30
wing disc CME-W2		18
wing disc ML-DmD8		7
wing disc ML-DmD9		5
wing disc ML-DmD16-c3		5
wing disc ML-DmD21		9
wing disc ML-DmD32		7
haltere disc ML-DmD17-c3		8
eye-antennal disc ML-DmD11		10
antennal disc ML-DmD20-c5		1
mixed discs ML-DmD4-c1		17
CNS ML-DmBG1-c1		0
CNS ML-DmBG2-c2		0
tumorous blood cells mbn2		1
ovary fGS/OSS		12
ovary OSC		8
ovary OSS		8
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		2
Schneider line 2 Sg4		(39)
embryonic 1182-4H		1
embryonic GM2		0
embryonic Kc167		0
embryonic S1		0
embryonic S3		(41)
leg disc CME L1		(30)
wing disc CME-W2		18
wing disc ML-DmD8		7
wing disc ML-DmD9		5
wing disc ML-DmD16-c3		5
wing disc ML-DmD21		9
wing disc ML-DmD32		7
haltere disc ML-DmD17-c3		8
eye-antennal disc ML-DmD11		10
antennal disc ML-DmD20-c5		1
mixed discs ML-DmD4-c1		17
CNS ML-DmBG1-c1		0
CNS ML-DmBG2-c2		0
tumorous blood cells mbn2		1
ovary fGS/OSS		12
ovary OSC		8
ovary OSS		8
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		2
Schneider line 2 Sg4		39
embryonic 1182-4H		1
embryonic GM2		0
embryonic Kc167		0
embryonic S1		0
embryonic S3		41
leg disc CME L1		30
wing disc CME-W2		18
wing disc ML-DmD8		7
wing disc ML-DmD9		5
wing disc ML-DmD16-c3		5
wing disc ML-DmD21		9
wing disc ML-DmD32		7
haltere disc ML-DmD17-c3		8
eye-antennal disc ML-DmD11		10
antennal disc ML-DmD20-c5		1
mixed discs ML-DmD4-c1		17
CNS ML-DmBG1-c1		0
CNS ML-DmBG2-c2		0
tumorous blood cells mbn2		1
ovary fGS/OSS		12
ovary OSC		8
ovary OSS		8
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		2
Schneider line 2 Sg4		39
embryonic 1182-4H		1
embryonic GM2		0
embryonic Kc167		0
embryonic S1		0
embryonic S3		41
leg disc CME L1		30
wing disc CME-W2		18
wing disc ML-DmD8		7
wing disc ML-DmD9		5
wing disc ML-DmD16-c3		5
wing disc ML-DmD21		9
wing disc ML-DmD32		7
haltere disc ML-DmD17-c3		8
eye-antennal disc ML-DmD11		10
antennal disc ML-DmD20-c5		1
mixed discs ML-DmD4-c1		17
CNS ML-DmBG1-c1		0
CNS ML-DmBG2-c2		0
tumorous blood cells mbn2		1
ovary fGS/OSS		12
ovary OSC		8
ovary OSS		8
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		2
Schneider line 2 Sg4		39
embryonic 1182-4H		1
embryonic GM2		0
embryonic Kc167		0
embryonic S1		0
embryonic S3		41
leg disc CME L1		30
wing disc CME-W2		18
wing disc ML-DmD8		7
wing disc ML-DmD9		5
wing disc ML-DmD16-c3		5
wing disc ML-DmD21		9
wing disc ML-DmD32		7
haltere disc ML-DmD17-c3		8
eye-antennal disc ML-DmD11		10
antennal disc ML-DmD20-c5		1
mixed discs ML-DmD4-c1		17
CNS ML-DmBG1-c1		0
CNS ML-DmBG2-c2		0
tumorous blood cells mbn2		1
ovary fGS/OSS		12
ovary OSC		8
ovary OSS		8
Expression Level Scale		Very low Low Moderate Moderately high High

log, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		2
Schneider line 2 Sg4		(39)
embryonic 1182-4H		1
embryonic GM2		0
embryonic Kc167		0
embryonic S1		0
embryonic S3		(41)
leg disc CME L1		30
wing disc CME-W2		18
wing disc ML-DmD8		7
wing disc ML-DmD9		5
wing disc ML-DmD16-c3		5
wing disc ML-DmD21		9
wing disc ML-DmD32		7
haltere disc ML-DmD17-c3		8
eye-antennal disc ML-DmD11		10
antennal disc ML-DmD20-c5		1
mixed discs ML-DmD4-c1		17
CNS ML-DmBG1-c1		0
CNS ML-DmBG2-c2		0
tumorous blood cells mbn2		1
ovary fGS/OSS		12
ovary OSC		8
ovary OSS		8
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		2
Schneider line 2 Sg4		39
embryonic 1182-4H		1
embryonic GM2		0
embryonic Kc167		0
embryonic S1		0
embryonic S3		41
leg disc CME L1		30
wing disc CME-W2		18
wing disc ML-DmD8		7
wing disc ML-DmD9		5
wing disc ML-DmD16-c3		5
wing disc ML-DmD21		9
wing disc ML-DmD32		7
haltere disc ML-DmD17-c3		8
eye-antennal disc ML-DmD11		10
antennal disc ML-DmD20-c5		1
mixed discs ML-DmD4-c1		17
CNS ML-DmBG1-c1		0
CNS ML-DmBG2-c2		0
tumorous blood cells mbn2		1
ovary fGS/OSS		12
ovary OSC		8
ovary OSS		8
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		2
Schneider line 2 Sg4		39
embryonic 1182-4H		1
embryonic GM2		0
embryonic Kc167		0
embryonic S1		0
embryonic S3		41
leg disc CME L1		30
wing disc CME-W2		18
wing disc ML-DmD8		7
wing disc ML-DmD9		5
wing disc ML-DmD16-c3		5
wing disc ML-DmD21		9
wing disc ML-DmD32		7
haltere disc ML-DmD17-c3		8
eye-antennal disc ML-DmD11		10
antennal disc ML-DmD20-c5		1
mixed discs ML-DmD4-c1		17
CNS ML-DmBG1-c1		0
CNS ML-DmBG2-c2		0
tumorous blood cells mbn2		1
ovary fGS/OSS		12
ovary OSC		8
ovary OSS		8
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Cell Line		Expression Level
Schneider line 2 S2R+
Schneider line 2 Sg4
embryonic 1182-4H
embryonic GM2
embryonic Kc167
embryonic S1
embryonic S3
leg disc CME L1
wing disc CME-W2
wing disc ML-DmD8
wing disc ML-DmD9
wing disc ML-DmD16-c3
wing disc ML-DmD21
wing disc ML-DmD32
haltere disc ML-DmD17-c3
eye-antennal disc ML-DmD11
antennal disc ML-DmD20-c5
mixed discs ML-DmD4-c1
CNS ML-DmBG1-c1
CNS ML-DmBG2-c2
tumorous blood cells mbn2
ovary fGS/OSS
ovary OSC
ovary OSS

modENCODE Treatments RNA-Seq

modENCODE Treatment Expression Data

(modENCODE_mRNA-Seq_treatments)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		4
cold shock, 4-day adult		2
heat shock, 4-day adult		10
Cadmium 50 mM 6 hrs, larvae L3		11
Cadmium 50 mM 12 hrs, larvae L3		7
Cadmium 50 mM 48 hrs, 4-day adult		13
Cadmium 100 mM 48 hrs, 4-day adult		5
Copper 0.5 mM 12 hrs, larvae L3		8
Copper 15 mM 48 hrs, 4-day adult		5
Zinc 5 mM 12 hrs, larvae L3		7
Zinc 4.5 mM 48 hrs, 4-day adult		8
Ethanol 2.5% 3 hrs, larvae L3		6
Ethanol 5% 3 hrs, larvae L3		13
Ethanol 10% 3 hrs, larvae L3		12
Caffeine 1.5 mg/ml 4 hrs, larvae L3		10
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		5
Caffeine 25 mg/ml 48 hrs, 4-day adult		3
Paraquat 5 mM 48 hrs, 4-day adult		9
Paraquat 10 mM 48 hrs, 4-day adult		11
Rotenone 2 μg 12 hrs, larvae L3		10
Rotenone 8 μg 12 hrs, larvae L3		13
Expression Level Scale		Very low Low Moderate

Linear, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		4
cold shock, 4-day adult		2
heat shock, 4-day adult		10
Cadmium 50 mM 6 hrs, larvae L3		11
Cadmium 50 mM 12 hrs, larvae L3		7
Cadmium 50 mM 48 hrs, 4-day adult		13
Cadmium 100 mM 48 hrs, 4-day adult		5
Copper 0.5 mM 12 hrs, larvae L3		8
Copper 15 mM 48 hrs, 4-day adult		5
Zinc 5 mM 12 hrs, larvae L3		7
Zinc 4.5 mM 48 hrs, 4-day adult		8
Ethanol 2.5% 3 hrs, larvae L3		6
Ethanol 5% 3 hrs, larvae L3		13
Ethanol 10% 3 hrs, larvae L3		12
Caffeine 1.5 mg/ml 4 hrs, larvae L3		10
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		5
Caffeine 25 mg/ml 48 hrs, 4-day adult		3
Paraquat 5 mM 48 hrs, 4-day adult		9
Paraquat 10 mM 48 hrs, 4-day adult		11
Rotenone 2 μg 12 hrs, larvae L3		10
Rotenone 8 μg 12 hrs, larvae L3		13
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		4
cold shock, 4-day adult		2
heat shock, 4-day adult		10
Cadmium 50 mM 6 hrs, larvae L3		11
Cadmium 50 mM 12 hrs, larvae L3		7
Cadmium 50 mM 48 hrs, 4-day adult		13
Cadmium 100 mM 48 hrs, 4-day adult		5
Copper 0.5 mM 12 hrs, larvae L3		8
Copper 15 mM 48 hrs, 4-day adult		5
Zinc 5 mM 12 hrs, larvae L3		7
Zinc 4.5 mM 48 hrs, 4-day adult		8
Ethanol 2.5% 3 hrs, larvae L3		6
Ethanol 5% 3 hrs, larvae L3		13
Ethanol 10% 3 hrs, larvae L3		12
Caffeine 1.5 mg/ml 4 hrs, larvae L3		10
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		5
Caffeine 25 mg/ml 48 hrs, 4-day adult		3
Paraquat 5 mM 48 hrs, 4-day adult		9
Paraquat 10 mM 48 hrs, 4-day adult		11
Rotenone 2 μg 12 hrs, larvae L3		10
Rotenone 8 μg 12 hrs, larvae L3		13
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		4
cold shock, 4-day adult		2
heat shock, 4-day adult		10
Cadmium 50 mM 6 hrs, larvae L3		11
Cadmium 50 mM 12 hrs, larvae L3		7
Cadmium 50 mM 48 hrs, 4-day adult		13
Cadmium 100 mM 48 hrs, 4-day adult		5
Copper 0.5 mM 12 hrs, larvae L3		8
Copper 15 mM 48 hrs, 4-day adult		5
Zinc 5 mM 12 hrs, larvae L3		7
Zinc 4.5 mM 48 hrs, 4-day adult		8
Ethanol 2.5% 3 hrs, larvae L3		6
Ethanol 5% 3 hrs, larvae L3		13
Ethanol 10% 3 hrs, larvae L3		12
Caffeine 1.5 mg/ml 4 hrs, larvae L3		10
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		5
Caffeine 25 mg/ml 48 hrs, 4-day adult		3
Paraquat 5 mM 48 hrs, 4-day adult		9
Paraquat 10 mM 48 hrs, 4-day adult		11
Rotenone 2 μg 12 hrs, larvae L3		10
Rotenone 8 μg 12 hrs, larvae L3		13
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		4
cold shock, 4-day adult		2
heat shock, 4-day adult		10
Cadmium 50 mM 6 hrs, larvae L3		11
Cadmium 50 mM 12 hrs, larvae L3		7
Cadmium 50 mM 48 hrs, 4-day adult		13
Cadmium 100 mM 48 hrs, 4-day adult		5
Copper 0.5 mM 12 hrs, larvae L3		8
Copper 15 mM 48 hrs, 4-day adult		5
Zinc 5 mM 12 hrs, larvae L3		7
Zinc 4.5 mM 48 hrs, 4-day adult		8
Ethanol 2.5% 3 hrs, larvae L3		6
Ethanol 5% 3 hrs, larvae L3		13
Ethanol 10% 3 hrs, larvae L3		12
Caffeine 1.5 mg/ml 4 hrs, larvae L3		10
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		5
Caffeine 25 mg/ml 48 hrs, 4-day adult		3
Paraquat 5 mM 48 hrs, 4-day adult		9
Paraquat 10 mM 48 hrs, 4-day adult		11
Rotenone 2 μg 12 hrs, larvae L3		10
Rotenone 8 μg 12 hrs, larvae L3		13
Expression Level Scale		Very low Low Moderate

log, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		4
cold shock, 4-day adult		2
heat shock, 4-day adult		10
Cadmium 50 mM 6 hrs, larvae L3		11
Cadmium 50 mM 12 hrs, larvae L3		7
Cadmium 50 mM 48 hrs, 4-day adult		13
Cadmium 100 mM 48 hrs, 4-day adult		5
Copper 0.5 mM 12 hrs, larvae L3		8
Copper 15 mM 48 hrs, 4-day adult		5
Zinc 5 mM 12 hrs, larvae L3		7
Zinc 4.5 mM 48 hrs, 4-day adult		8
Ethanol 2.5% 3 hrs, larvae L3		6
Ethanol 5% 3 hrs, larvae L3		13
Ethanol 10% 3 hrs, larvae L3		12
Caffeine 1.5 mg/ml 4 hrs, larvae L3		10
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		5
Caffeine 25 mg/ml 48 hrs, 4-day adult		3
Paraquat 5 mM 48 hrs, 4-day adult		9
Paraquat 10 mM 48 hrs, 4-day adult		11
Rotenone 2 μg 12 hrs, larvae L3		10
Rotenone 8 μg 12 hrs, larvae L3		13
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		4
cold shock, 4-day adult		2
heat shock, 4-day adult		10
Cadmium 50 mM 6 hrs, larvae L3		11
Cadmium 50 mM 12 hrs, larvae L3		7
Cadmium 50 mM 48 hrs, 4-day adult		13
Cadmium 100 mM 48 hrs, 4-day adult		5
Copper 0.5 mM 12 hrs, larvae L3		8
Copper 15 mM 48 hrs, 4-day adult		5
Zinc 5 mM 12 hrs, larvae L3		7
Zinc 4.5 mM 48 hrs, 4-day adult		8
Ethanol 2.5% 3 hrs, larvae L3		6
Ethanol 5% 3 hrs, larvae L3		13
Ethanol 10% 3 hrs, larvae L3		12
Caffeine 1.5 mg/ml 4 hrs, larvae L3		10
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		5
Caffeine 25 mg/ml 48 hrs, 4-day adult		3
Paraquat 5 mM 48 hrs, 4-day adult		9
Paraquat 10 mM 48 hrs, 4-day adult		11
Rotenone 2 μg 12 hrs, larvae L3		10
Rotenone 8 μg 12 hrs, larvae L3		13
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		4
cold shock, 4-day adult		2
heat shock, 4-day adult		10
Cadmium 50 mM 6 hrs, larvae L3		11
Cadmium 50 mM 12 hrs, larvae L3		7
Cadmium 50 mM 48 hrs, 4-day adult		13
Cadmium 100 mM 48 hrs, 4-day adult		5
Copper 0.5 mM 12 hrs, larvae L3		8
Copper 15 mM 48 hrs, 4-day adult		5
Zinc 5 mM 12 hrs, larvae L3		7
Zinc 4.5 mM 48 hrs, 4-day adult		8
Ethanol 2.5% 3 hrs, larvae L3		6
Ethanol 5% 3 hrs, larvae L3		13
Ethanol 10% 3 hrs, larvae L3		12
Caffeine 1.5 mg/ml 4 hrs, larvae L3		10
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		5
Caffeine 25 mg/ml 48 hrs, 4-day adult		3
Paraquat 5 mM 48 hrs, 4-day adult		9
Paraquat 10 mM 48 hrs, 4-day adult		11
Rotenone 2 μg 12 hrs, larvae L3		10
Rotenone 8 μg 12 hrs, larvae L3		13
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Treatment		Expression Level
extended cold, 4-day adult
cold shock, 4-day adult
heat shock, 4-day adult
Cadmium 50 mM 6 hrs, larvae L3
Cadmium 50 mM 12 hrs, larvae L3
Cadmium 50 mM 48 hrs, 4-day adult
Cadmium 100 mM 48 hrs, 4-day adult
Copper 0.5 mM 12 hrs, larvae L3
Copper 15 mM 48 hrs, 4-day adult
Zinc 5 mM 12 hrs, larvae L3
Zinc 4.5 mM 48 hrs, 4-day adult
Ethanol 2.5% 3 hrs, larvae L3
Ethanol 5% 3 hrs, larvae L3
Ethanol 10% 3 hrs, larvae L3
Caffeine 1.5 mg/ml 4 hrs, larvae L3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
Caffeine 25 mg/ml 48 hrs, 4-day adult
Paraquat 5 mM 48 hrs, 4-day adult
Paraquat 10 mM 48 hrs, 4-day adult
Rotenone 2 μg 12 hrs, larvae L3
Rotenone 8 μg 12 hrs, larvae L3

Expression Clusters

A cluster of genes with similar mRNA expression dynamics across development.

External Data & Images

abdominal 2 lateral longitudinal muscle 1

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0001250

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG9623-RB

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

lethal

lethal, with Scer\GAL4^elav.PLu

if^GD1175

lethal, with Scer\GAL4^Mef2.PR

if^GD1175

lethal | embryonic stage

lethal | pupal stage, with Scer\GAL4^pnr-MD237

if^GD1175

lethal | recessive

partially lethal - majority die | pupal stage, with Scer\GAL4^pnr-MD237

if^GD1175

viable

viable (with if^2B1)

if^SEF

viable (with if^C2B)

if^SEF

viable (with if^SEF)

viable | poor

if¹

Sterility

Allele

fertile

fertile | poor

if¹

Other Phenotypes

Allele

neuroanatomy defective

if^k27e

visible

visible | dominant

visible | heat sensitive, with Scer\GAL4^337Y

visible | recessive

if³

visible | recessive | cold sensitive

if³

wild-type

Phenotype manifest in

Allele

if^B4

abdominal 2 segment border muscle

if^B4

abdominal 2 ventral longitudinal muscle 1

if^B4

abdominal 3 lateral longitudinal muscle 1

if^B4

abdominal 3 segment border muscle

if^B4

abdominal 3 ventral longitudinal muscle 1

if^B4

abdominal 4 lateral longitudinal muscle 1

if^B4

abdominal 4 segment border muscle

if^B4

abdominal 4 ventral longitudinal muscle 1

if^B4

abdominal 5 lateral longitudinal muscle 1

if^B4

abdominal 5 segment border muscle

if^B4

abdominal 5 ventral longitudinal muscle 1

if^B4

abdominal 6 lateral longitudinal muscle 1

if^B4

abdominal 6 segment border muscle

if^B4

abdominal 6 ventral longitudinal muscle 1

if^B4

abdominal 7 lateral longitudinal muscle 1

if^B4

abdominal 7 segment border muscle

if^B4

abdominal 7 ventral longitudinal muscle 1

if^B4

actin cytoskeleton | embryonic stage 17

anterior crossvein

if³

border follicle cell, with Scer\GAL4^slbo.2.6

if^NIG.9623R

crossvein

embryonic/first instar larval cuticle | dorsal

embryonic/larval midgut

embryonic/larval midgut | embryonic stage

embryonic/larval proventriculus | embryonic stage

if^B4

embryonic/larval somatic muscle | embryonic stage 17

embryonic/larval visceral branch

if^B4

embryonic/larval visceral muscle

extended germ band embryo

eye, with Scer\GAL4^how-24B

if^C.Scer\UAS

filopodium & abdominal ventral longitudinal muscle 3

if^k27e

germ layer | embryonic stage

if^k27e

hemocyte | embryonic stage

if^B2

intersegmental nerve

if^k27e

midgut constriction

muscle attachment site

if^C2B

muscle attachment site & basal lamina

if^B4

muscle attachment site & connecting hemi-adherens junction

if^B4

muscle attachment site & muscle tendon junction

if^B4

muscle attachment site | embryonic stage

if^B2

presumptive embryonic/larval central nervous system

presumptive embryonic/larval digestive system

if^B4

presumptive embryonic/larval muscle system

if^SEF

presumptive embryonic/larval peripheral nervous system

presumptive embryonic salivary gland

if^k27e

proventriculus inner layer

if^B4

RP3 motor neuron

if^k27e

salivary gland common duct primordium

sarcomere & somatic muscle

segmental nerve

if^k27e

somatic muscle

somatic muscle | larval stage

if^2B1

stage S14 oocyte

if^B2

visceral mesoderm

wing

wing, with Scer\GAL4⁶⁸⁴

if^m8.Scer\UAS

wing | cold sensitive

if³

wing | heat sensitive, with Scer\GAL4^337Y

wing | somatic clone

if^A7

wing blade

if³

wing vein

Classical Alleles ( 54 )

For All Classical Alleles Show

Allele of if	Class	Mutagen	Stocks	Known lesion
if^B2	loss of function allele, amorphic allele - genetic evidence, hypomorphic allele - genetic evidence	ethyl methanesulfonate	2	--
if^k27e	amorphic allele - genetic evidence, loss of function allele	ethyl methanesulfonate	2	--
if³		spontaneous	2	--
if^MB10541			1	--
if¹⁶	amorphic allele - genetic evidence	ethyl methanesulfonate	0	--
if²⁶	amorphic allele - genetic evidence	X ray	0	--
if^2B2	amorphic allele - genetic evidence	ethyl methanesulfonate	0	--
if^2B3	amorphic allele - genetic evidence	ethyl methanesulfonate	0	--
if³⁸	amorphic allele - genetic evidence	ethyl methanesulfonate	0	--
if^4.5	amorphic allele - genetic evidence	ethyl methanesulfonate	0	--
if⁶⁴⁸	amorphic allele - genetic evidence	ethyl methanesulfonate	0	--
if⁶⁴	amorphic allele - genetic evidence	X ray	0	--
if⁶	amorphic allele - genetic evidence	X ray	0	--
if⁸	amorphic allele - genetic evidence	X ray	0	--
if⁹	amorphic allele - genetic evidence	ethyl methanesulfonate	0	--
if^A2	amorphic allele - genetic evidence	ethyl methanesulfonate	0	--
if^A7	loss of function allele, amorphic allele - genetic evidence	ethyl methanesulfonate	0	--
if^B4	loss of function allele, amorphic allele - genetic evidence	gamma ray	0	Yes
if^B6	amorphic allele - genetic evidence	gamma ray	0	Yes
if^C1A	amorphic allele - genetic evidence	ethyl nitrosourea	0	--
if^C3C	amorphic allele - genetic evidence	ethyl nitrosourea	0	--
if^C4D	amorphic allele - genetic evidence	ethyl methanesulfonate	0	--
if^C5E	amorphic allele - genetic evidence	ethyl methanesulfonate	0	--
if^K13	amorphic allele - genetic evidence	ethyl methanesulfonate	0	--
if^K23	amorphic allele - genetic evidence	ethyl methanesulfonate	0	--
if^L10	amorphic allele - genetic evidence	ethyl methanesulfonate	0	--
if^L13	amorphic allele - genetic evidence	ethyl methanesulfonate	0	--
if^L326	amorphic allele - genetic evidence	ethyl methanesulfonate	0	--
if^L6	amorphic allele - genetic evidence	ethyl methanesulfonate	0	--
if^M2	amorphic allele - genetic evidence	ethyl methanesulfonate	0	--
if^N18R	amorphic allele - genetic evidence	ethyl methanesulfonate	0	--
if^N19S	amorphic allele - genetic evidence	ethyl methanesulfonate	0	--
if^O11K	amorphic allele - genetic evidence	ethyl methanesulfonate	0	--
if^O17Q	amorphic allele - genetic evidence	ethyl methanesulfonate	0	--
if^O2	amorphic allele - genetic evidence	ethyl methanesulfonate	0	--
if^O2B	amorphic allele - genetic evidence	ethyl methanesulfonate	0	--
if^O9I	amorphic allele - genetic evidence	ethyl methanesulfonate	0	--
if^Q2B	amorphic allele - genetic evidence	ethyl methanesulfonate	0	--
if^13ts	hypomorphic allele - genetic evidence	ethyl methanesulfonate	0	--
if¹⁷	hypomorphic allele - genetic evidence	ethyl methanesulfonate	0	Yes
if¹		spontaneous	0	--
if²¹	hypomorphic allele - genetic evidence	ethyl methanesulfonate	0	Yes
if²			0	--
if^2B1		ethyl methanesulfonate	0	Yes
if³⁵	hypomorphic allele - genetic evidence	ethyl methanesulfonate	0	Yes
if^815-17		X ray	0	--
if^B2.1		gamma ray	0	--
if^C2B		ethyl nitrosourea	0	Yes
if^CPTI004152		piggyBac transposase	0	Yes
if^GG01583			0	--
if^H18			0	--
if^N		neutrons	0	--
if^SEF		ethyl methanesulfonate	0	Yes
if^unspecified			0	--

Alleles Carried on Transgenic Constructs ( 31 )

For All Alleles Carried on Transgenic Constructs Show

Allele of if	Mutagen	Stocks	Known lesion
if^GD1175	in vitro construct - RNAi in vitro construct - regulatory fusion	2	Yes
if^HMS01872	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
if^JF02695	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
if^KK108544	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
if^+t39	in vitro construct - genomic fragment	0	Yes
if^+tBa	in vitro construct - deletion	0	Yes
if^C.hs	in vitro construct - regulatory fusion	0	Yes
if^C.Scer\UAS	in vitro construct - regulatory fusion	0	Yes
if^GFANA.hs	in vitro construct - regulatory fusion in vitro construct - amino acid replacement	0	Yes
if^GOF.C.hs	in vitro construct - deletion	0	Yes
if^GOF.m8.hs	in vitro construct - deletion	0	Yes
if^{GOF.m8.Scer\UAS}	in vitro construct - deletion	0	Yes
if^LOF.C.hs	in vitro construct - amino acid replacement	0	Yes
if^LOF.m8.hs	in vitro construct - amino acid replacement	0	Yes
if^{LOF.m8.Scer\UAS}	in vitro construct - amino acid replacement	0	Yes
if^m8.hs	in vitro construct - regulatory fusion	0	Yes
if^{m8.Scer\FRT.αTub84B}	in vitro construct - regulatory fusion	0	Yes
if^m8.Scer\UAS	in vitro construct - regulatory fusion	0	Yes
if^m8.αTub84B	FLPase	0	Yes
if^minigene	in vitro construct - deletion	0	Yes
if^MtnA.PG	in vitro construct - regulatory fusion	0	Yes
if^NIG.9623R	in vitro construct - RNAi in vitro construct - regulatory fusion	0	Yes
if^{Scer\UAS.cMBa}	in vitro construct - regulatory fusion	0	Yes
if^Scer\UAS.cUa	in vitro construct - regulatory fusion	0	Yes
if^Δ.C.hs	in vitro construct - deletion	0	Yes
if^{Δ.C.Scer\UAS}	in vitro construct - deletion	0	Yes
if^Δ.m8.hs	in vitro construct - deletion	0	Yes
if^{Δ.m8.Scer\UAS}	in vitro construct - deletion	0	Yes
if^{Δcyt.Scer\UAS}	in vitro construct - regulatory fusion in vitro construct - deletion	0	Yes
if^{ΔGFFNR.Scer\UAS}	in vitro construct - regulatory fusion in vitro construct - deletion	0	Yes
if^ΔGFFNR	in vitro construct - deletion	0	Yes

Aneuploid Aberrations

Disrupted in

Ab(1;?)if^B2.1

(Christensen et al., 2008.9.3)

Df(1)BSC581

Df(1)BSC582

(Christensen et al., 2008.9.3)

Df(1)BSC593

(Christensen et al., 2008.9.3)

Df(1)BSC725

(Christensen et al., 2008.12.28)

Df(1)BSC762

(Christensen et al., 2009.2.28)

Df(1)ED7374

(Roote, 2004.6.24, Ryder and Roote, 2004.11.10)

Df(1)r-D1

(Schalet, 1986)

Df(1)rif

(Bloor and Brown, 1998, Walsh and Brown, 1998, Brown, 1994)

T(1;2)if^B6

T(1;4)if^V2

(Popodi et al., 2010-, Venken et al., 2010)

Duplicated in

Dp(1;3)DC319

Dp(1;4)80f3c

Dp(1;4)fK4

Dp(1;4)fK7

Dp(1;4)fK24

Dp(1;4)fK27

Dp(1;4)r⁺l

Dp(1;Y)BSC201

(Cook et al., 2010)

Not disrupted in

Df(1)815-6

Df(1)B

Df(1)BK8

Df(1)BK10

Df(1)BK16

Df(1)BK17

Df(1)BK18

Df(1)BK28

T(1;4)if^V2

Not duplicated in

Dp(1;3)f⁺71b

Dp(1;4)rK20

Dp(1;Y)W73

P{Mae-UAS.6.11}if^GG01583

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{UAS-if::mew.αPS1/2}

P{UAS-if::mew.αPS2/1}

reporter construct

characterization construct

P{αTub84B(FRT.y⁺)if.m8}

P{αTub84B(-FRT)if.m8}

Insertions

Type of insertions

Name

Expression data

insertion of mobile activating element

insertion of enhancer trap binary system

Mi{ET1}if^MB10541

miscellaneous insertions

PBac{806.LOX-SVS-2}if^CPTI004152

Gene Ontology: Function, Process & Cellular Component ( 27 unique terms )

Terms Based on Experimental Evidence ( 17 terms )

Molecular Function

CV term

cell adhesion molecule binding

References

inferred from direct assay

receptor activity

inferred from direct assay

Biological Process

CV term

(Stevens and Jacobs, 2002)

References

axonal defasciculation

inferred from mutant phenotype

(Huang et al., 2007)

axon guidance

inferred from mutant phenotype

border follicle cell migration

inferred from mutant phenotype

(Kókai et al., 2012)

cell adhesion

inferred from direct assay

inferred from mutant phenotype

imaginal disc-derived wing morphogenesis

hemocyte migration

inferred from mutant phenotype

(Siekhaus et al., 2010)

inferred from expression pattern AND inferred from mutant phenotype

(Brabant et al., 1996)

maintenance of epithelial integrity, open tracheal system

inferred from genetic interaction with mys

(Levi et al., 2006)

maintenance of protein location

inferred from mutant phenotype

(Bloor and Kiehart, 2001, Devenport et al., 2007)

muscle attachment

inferred from genetic interaction with Grip

(Estrada et al., 2007)

inferred from mutant phenotype

myofibril assembly

inferred from mutant phenotype

regulation of cell shape

inferred from mutant phenotype

salivary gland morphogenesis

inferred from genetic interaction with βTub85D

(Jattani et al., 2009)

sensory perception of smell

inferred from genetic interaction with sws

(Ayyub and Paranjape, 2002)

Cellular Component

CV term

(Tanentzapf et al., 2006)

References

plasma membrane

inferred from direct assay

sarcolemma

inferred from direct assay

Terms Based on Predictions or Assertions ( 13 terms )

Molecular Function

CV term

cell adhesion molecule binding

References

inferred from sequence or structural similarity

receptor activity

inferred from sequence or structural similarity

Biological Process

CV term

apposition of dorsal and ventral imaginal disc-derived wing surfaces

References

non-traceable author statement

(Brower, 2003)

cell-cell adhesion

traceable author statement

cell-matrix adhesion

inferred from sequence or structural similarity

cell migration

traceable author statement

epithelial cell migration, open tracheal system

traceable author statement

(Rosin and Shilo, 2002)

heterophilic cell-cell adhesion

traceable author statement

imaginal disc-derived wing morphogenesis

traceable author statement

midgut development

traceable author statement

salivary gland development

traceable author statement

(Abrams et al., 2003)

tracheal outgrowth, open tracheal system

traceable author statement

(Ghabrial et al., 2003)

Cellular Component

CV term

(FlyBase Curators et al., 2004-)

References

integrin complex

inferred from electronic annotation with InterPro:IPR000413

inferred from sequence or structural similarity

(Brower, 2003, Swiss-Prot Project Members, 1989.10.1)

non-traceable author statement

traceable author statement

(Roote and Zusman, 1995, Martin-Bermudo et al., 1997, Prokop et al., 1998, Martin-Bermudo et al., 1999)

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Interactions & Pathways

Summary of Physical Interactions

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

if allele

Gene

References

if^B4

mew

if^C.Scer\UAS

mew

(Roote and Zusman, 1996, Martin-Bermudo et al., 1997, Li et al., 1998)

(Roote and Zusman, 1995, Li et al., 1998)

sli

(Stevens and Jacobs, 2002)

(Roote and Zusman, 1996, Martin-Bermudo et al., 1997, Li et al., 1998)

(Martin-Bermudo et al., 1997)

if^unspecified

mys

(Jannuzi et al., 2002)

unspecified

(Whalen and Simon, 2001)

(Mueller et al., 2005)

mew

(Levi et al., 2006)

mys

(Bloor and Brown, 1998, Jannuzi et al., 2004)

rhea

(Brown et al., 2002)

rho

(Sturtevant and Bier, 1995)

Zasp52

(Jani and Schock, 2007)

External Data

y¹ v¹; P{TRiP.JF02695}attP2

BioGRID - A database of protein and genetic interactions

•

58994

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0001250

InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets.

•

32661

Orthologs

OrthoDB Orthologs (34) - based on analysis using Dmel annotation version 5.41

OrthoDB Ortholog Groups

OrthoDB orthology group searches

(Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288)

Drosophila inclusive ortholog search

EOG6RJGKV

Dipteran inclusive ortholog search

EOG67WNWN

Insect inclusive ortholog search

EOG63TXQK

Arthropod inclusive ortholog search

EOG6CFXQ8

Metazoa inclusive ortholog search

EOG6VTJQ6

Orthologs in Drosophila Species (EOG6RJGKV)

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA21920

Drosophila persimilis

Dper\GL26767

Drosophila willistoni

Dwil\GK18412

Drosophila virilis

Dvir\GJ18848

Drosophila mojavensis

Dmoj\GI15956

Drosophila grimshawi

Dgri\GH12801

Orthologs in non-Drosophila Dipterans (EOG67WNWN)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL013600

Anopheles gambiae

Malaria mosquito

Agam\AGAP000032

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ017320

Orthologs in non-Dipteran Insects (EOG63TXQK)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Apis mellifera

Western honey bee

Amel\GB49921

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG012335

Acromyrmex echinatior

Panamanian leafcutter ant

Aech\AECH20346

Atta cephalotes

Leafcutter ant

Acep\ACEP25232

Camponotus floridanus

Florida carpenter ant

Cflo\CFLO14460

Harpegnathos saltator

Jerdons jumping ant

Hsal\HSAL14952

Linepithema humile

Argentine ant

Lhum\LH16573

Pogonomyrmex barbatus

Red harvester ant

Pbar\PB20828

Solenopsis invicta

Red fire ant

Sinv\SINV13588

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI009808

Bombyx mori

Silkmoth

Bmor\BGIBMGA006655

Pediculus humanus

Human body louse

Phum\PHUM467160

Tribolium castaneum

Red flour beetle

Tcas\TC001667

Orthologs in non-Insect Arthropods (EOG6CFXQ8)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Daphnia pulex

Water flea

Dpux\JGI_V11_304328

Orthologs in non-Arthropod Metazoa (EOG6VTJQ6)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Caenorhabditis elegans

Nematode

Cele\pat-2

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_013536gn

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_020175gn

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_015921gn

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_008204gn

Human Orthologs (0)

Gene

OMIM

HGNC

AAA Orthologs (11) based on analysis using Dmel annotation version 4.3

Organism

Gene

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA21920

Drosophila persimilis

Dper\GL26767

Drosophila willistoni

Dwil\GK18412

Drosophila virilis

Dvir\GJ18848

Drosophila mojavensis

Dmoj\GI15956

Drosophila grimshawi

Dgri\GH12801

Stocks & Reagents

Stocks Listed in FlyBase ( 12 )

Bloomington

3960

if³

29896

w¹¹¹⁸ Mi{ET1}if^MB10541

2176

g² if^B2 f^36a/FM7c

27544

2177

g² if^k27e f^36a/FM7c

38958

y¹ sc^* v¹; P{TRiP.HMS01872}attP40/CyO

Kyoto

107534

if³

106685

g² if^B2 f^36a/FM7c

106686

g² if^k27e f^36a/FM7c

VDRC

v100770

P{KK108544}VIE-260B

v5600

w¹¹¹⁸ P{GD1175}v5600

v44885

w¹¹¹⁸; P{GD1175}v44885

Genomic Clones ( 2 )

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones ( 48 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

AT02663

Other clones

RNAi & Array Information

DRSC - Results from RNAi screens.

•

FBgn0001250

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

32661

Antibody Information

monoclonal

(Gullberg et al., 1994)

monoclonal, polyclonal

Other Information

Discoverer

Etymology

Identification

Relationship to Other Genes

Source for database identity of

Source for database merge of

Additional comments

Other Comments

ChEST reveals this is a target of Mef2.

(Junion et al., 2005)

RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in S2R⁺ cells: cells become round and detached. Kc167 cells are unaffected.

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Martin-Bermudo et al., 1999)

if is required in the visceral mesoderm for normal migration of the endodermal midgut cells over the visceral mesoderm in the developing embryo.

Class I and class II alleles fully complement class IV alleles. Class II alleles fully complement class III alleles. if^13ts class I allele fully complements class II alleles, but the transheterozygotes between other class I alleles and class III allele generally die, although a few adult escapers are observed.

Mutational analysis reveals if is required for the development of the adult halteres and legs, as well as the wing. if is required for the formation of the somatic muscle sarcomeric structure, rather than for its maintenance. The function of if in the morphogenesis of the midgut and nerve cord is distinct from its function in muscle attachment and sarcomeric structure.

(Martin-Bermudo et al., 1998)

The functional significance of the cytoplasmic domains of the if, mew and mys subunits of the Position Specific (PS) integrin family are studied by analysing the relationship between the cytoplasmic domain structure and function in the context of a developing organism. The cytoplasmic tail of if is essential for both embryonic and postembryonic processes.

(Li et al., 1998)

Modulation of integrin function through the cytoplasmic domain of if is essential for embryonic morphogenesis.

if is essential for the adhesion of muscle and epidermal hemiadherens junctions to extracellular matrix at muscle attachment sites in the developing embryo.

(Prokop et al., 1998)

The ability of two different integrin α subunits (encoded by mew and if) to substitute for each other during embryonic development has been studied. The two α subunits encoded by mew and if are not equivalent and have distinct functions which reside in the extracellular domains.

(Martin-Bermudo et al., 1997)

Clonal analysis demonstrates that the different integrins, mew and if, are required on opposite wing epithelia. An early integrin-dependent process, not obviously required for prepupal adhesion, is essential to permit subsequent wing morphogenesis.

(Brabant et al., 1996)

if and mys can be localised by an intracellular mechanism within the muscles. Direct localisation of the transmembrane protein to sites of integrin function occurs in cells that lack endogenous mys and if or cells that lack extracellular signals from the tendon cells.

(Martin-Bermudo and Brown, 1996)

Either splice form of if is sufficient for viability, but they do have distinct requirements during development. if function can replace mew function in the retina.

(Roote and Zusman, 1996)

Phenotypic analysis of mew, if and mys embryos suggests multiple roles for PS integrins in the adhesion of cells and in the formation, organization and migration of embryonic tissues. Although the proteins are often expressed in adjacent embryonic tissues, this distribution does not necessarily reflect equivalent requirements. The complete loss of both α subunits, encoded by mew and if, does not produce all the phenotypes observed in embryos lacking the mys encoded β subunit.

(Roote and Zusman, 1995)

Molecular and genetic analysis prove that the α_PS2 integrin subunit is encoded by the if locus. Comparison of the null phenotypes of mys (encoding the Β_PS integrin subunit) and if rules out a model where PS integrin function occurs solely by the direct interaction of the two PS integrins, α_PS1Β_PS and α_PS2Β_PS.

(Prokop et al., 1997, Hoang and Chiba, 1998)

if has been expressed on the surface of Drosophila tissue culture cells. if gene product promotes cell spreading on two different extra cellular matrix (ECM) molecules, laminin and tiggin.

(Gotwals et al., 1994)

Muscle phenotype of mutants studied using polarised light microscopy and antibody staining to detect Mhc-lacZ reporter gene expression in muscles.

(Drysdale et al., 1993)

The distribution of mew, if and scb proteins in cultured cell lines has been investigated.

(Peel and Milner, 1992)

Scaffold attachment regions in the region surrounding if have been mapped.

(Surdej et al., 1990)

if gene product is the PS2α integrin subunit. if is required to ensure the correct apposition and patterning of the wing epithelia.

(Wilcox et al., 1989)

Structural gene for the α-subunit of position specific integrin 2 (PS2), a large transmembrane protein (Bogaert, Brown and Wilcox, 1987). The β-subunit can associate with either of the two α-subunits, PS1 or PS2 (Brower et al., 1984; Wilcox et al., 1984). Both α and β integrin are expressed in embryonic and larval tissues. In early development, PS2 is found in the mesoderm, localized to muscle attachments (Bogaert, Brown and Wilcox, 1987). Later, PS1 is expressed in the presumptive dorsal epithelium of the third instar imaginal wing discs; also, PS2 is found in the ventral epithelium, both integrins being important for the joining of the dorsal and ventral surfaces of the wing blade (Brower and Jaffe, 1989). Null mutations cause embryonic lethality (Wilcox, DiAntonio and Leptin, 1989). In the mutant if¹, the adult wing is inflated with lymph and smaller than normal; venation is defective. Wings later become dry and blistered.

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

RefSeq (Proteins)

•

DDBJ / EMBL / GenBank

•

Entrez Gene - A searchable database of RefSeq genes.

•

32661

UniProtKB/Swiss-Prot

•

P12080

UniProtKB/TrEMBL

•

Other Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Integrin alpha chain, C-terminal cytoplasmic region, conserved site (IPR018184)

Integrin alpha chain (IPR000413)

Integrin alpha beta-propellor (IPR013519)

Integrin alpha-2 (IPR013649)

FG-GAP repeat (IPR013517)

Linkouts

BioGRID - A database of protein and genetic interactions

•

58994

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0001250

DRSC - Results from RNAi screens.

•

FBgn0001250

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0001250

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG9623-RB

FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans

•

FBgn0001250

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

32661

InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets.

•

32661

modMine - Data generated by the modENCODE project.

•

FBgn0001250

REDfly - A database of transcriptional regulatory elements.

•

FBgn0001250

Synonyms & Secondary IDs ( 29 )

Reported As

Symbol Synonym

alphaPS2

(Bouge et al., 2005)

aPS2

CG9623

(Klebes et al., 2005, Gonzalez-Aguero et al., 2005, Schiess et al., 2009, Keleman et al., 2009.8.5, Perkins et al., 2009, Macdonald and Long, 2006, Cammarato et al., 2011, Ni et al., 2010.12.1)

CT27194

(Chanana et al., 2007, Devenport et al., 2007, Dietzl et al., 2007, Tsai et al., 2008, Christensen et al., 2008.9.3, Christensen et al., 2008.9.3, Christensen et al., 2008.9.3, Ritzenthaler and Chiba, 2003, Huang et al., 2007, Delon and Brown, 2009, Christensen et al., 2008.12.28, O'Reilly et al., 2008, Estrada et al., 2007, Inoue and Hayashi, 2007, Christensen et al., 2009.2.28, Liu et al., 2009, Junion et al., 2007, Dinkins et al., 2008, Jattani et al., 2009, Gilsohn and Volk, 2010, Popodi et al., 2010-, Fraichard et al., 2010, Zhang et al., 2010, Tikhmyanova et al., 2010, Beltran et al., 2007, Zhang et al., 2008, Zervas et al., 2011, Perkins et al., 2010, Negreiros et al., 2010, Chen et al., 2008, Brown et al., 2013.2.5, Kókai et al., 2012, Kim et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Venken et al., 2010, Cook et al., 2010)

(Tikhmyanova et al., 2010)

(Bryant, 1997)

inf