Gene Dmel\ImpE1

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General Information

Symbol

Dmel\ImpE1

Species

D. melanogaster

Name

Ecdysone-inducible gene E1

Annotation symbol

CG32356

Feature type

protein_coding_gene

FlyBase ID

FBgn0001253

Gene Model Status

Current

Stock availability

6 publicly available

Also Known As

IMP-E1, CG7116

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

66C8-66C10

Sequence location

3L:8,365,505..8,384,271 [+]

Genomic Maps

Select View:

Summary Information

Automatically generated summary

See sections below for more information

The gene Ecdysone-inducible gene E1 is referred to in FlyBase by the symbol Dmel\ImpE1 (CG32356, FBgn0001253). It is a protein_coding_gene from Drosophila melanogaster. Its molecular function is unknown. It is reported to be involved in the biological process: imaginal disc eversion. 5 alleles are reported. No phenotypic data is available. It has 3 annotated transcripts and 3 annotated polypeptides. Protein features are: EB domain; Low-density lipoprotein (LDL) receptor class A repeat; Low-density lipoprotein (LDL) receptor class A, conserved site. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of extremely low expression. Peak expression observed within 12-18 embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Two or more Affy2 ProbeSets identify exons of this gene. This is a summary of the tissue expression peaks exhibited in at least one of these ProbeSets. Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system. Gene sequence location is 3L:8365505..8384271.

User Contributed Data

Beta version, feedback welcome

Phenotypic Description from the Red Book (Lindsley & Zimm 1992)

Gene/Allele symbols may differ from current usage

Imp-E: Inducible membrane-bound polysomes-Early

Name given to three genes encoding transcripts associated with membrane-bound polysomes in imaginal discs and expressed only in response to 20-hydroxyecdysone (20 HOE). Expression studied both in vitro and in vivo. Imp-E denotes three "early" genes involved in elongation of appendage-forming regions of the imaginal discs by means of epithelial-cell rearrangements (Natzle et al., 1988).

Recent Updates

Description

What does this section display?

This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).

What does this section not display?

This section does not currently display links that were removed or gene model changes.

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FB2013_03

FB2013_02

All updates

Click here to see a list of all updates to this record from FB2010_08 and on.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

66C8-66C10

Limits computationally determined from genome sequence between P{lacW}l(3)L0139^L0139 and P{PZ}Gug⁰³⁹²⁸&P{EP}CG6745^EP595b

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

66C7-66C10

(Robertson, 1999.12.17)

66C-66C

(determined by in situ hybridisation)

(Natzle et al., 1986)

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Gene Model & Products

Please see the GBrowse view of Dmel\ImpE1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Comments on Gene Model

Gene model reviewed during 5.46

Low-frequency RNA-Seq exon junction(s) not annotated.

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

ImpE1-RA

FBtr0076606

NM_080110

6043

1616

ImpE1-RB

FBtr0076607

NM_168270

919

146

ImpE1-RC

FBtr0333057

NM_001274648

6099

1616

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

ImpE1-PA

FBpp0076333

177.0

1616

3.74

NP_524849

AAF50429

ImpE1-PB

FBpp0076334

16.0

146

8.15

NP_729371

AAF50430

ImpE1-PC

FBpp0305271

177.0

1616

3.74

NP_001261577

AGB94272

Additional Polypeptide Data & Comments

Reported size (kDa)

Comments

External Data

Linkouts

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

EB domain (IPR006149)

Low-density lipoprotein (LDL) receptor class A, conserved site (IPR023415)

Low-density lipoprotein (LDL) receptor class A repeat (IPR002172)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Mapped Features

Mapped Features have been reorganized, please see this article for details.

Additional mapped features and mutations can be found on GBrowse or related reports.

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

Crossreferences

Expression Data

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 9 -- 10

inclusive hindgut primordium

(Fisher et al., 2012)

embryonic stage 11 -- 12

hindgut proper primordium

(Fisher et al., 2012)

embryonic stage 13 -- 16

atrium

(Fisher et al., 2012)

embryonic dorsal epidermis

(Fisher et al., 2012)

embryonic esophagus

(Fisher et al., 2012)

embryonic foregut

(Fisher et al., 2012)

embryonic head epidermis

(Fisher et al., 2012)

embryonic hindgut

(Fisher et al., 2012)

embryonic ventral epidermis

(Fisher et al., 2012)

embryonic/larval dorsal trunk

(Fisher et al., 2012)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

third instar - uncleared gut stage

embryonic/larval central nervous system

(Restifo and Hauglum, 1998)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization (GO Cellular Component)

CV term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

Reference

See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.

FlyAtlas Anatomy Microarray

FlyAtlas Anatomical Expression Data

(FlyAtlas-RNA.adult)

(FlyAtlas-RNA.larva)

	Styles
	Linear
	Logarithmic
	Heatmap
	Back-to-back
	Scales
	gene max expression
	Moderate expression bin max
	High level expression bin max
	Very high expression bin max

Summary of FlyAtlas Anatomical Expression Data: Two or more Affy2 ProbeSets identify exons of this gene. This is a summary of the tissue expression peaks exhibited in at least one of these ProbeSets. Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system.
[download data (TSV)]

Guide to FlyAtlas expression level colors
	No expression (0 - 9.999)
	Low expression (10 - 99.999)
	Moderate expression (100 - 499.999)
	High level expression (500 - 999.999)
	Very high expression (>999.999)

Heatmap
Tissue		Expression Level
Larval Central Nervous System
Larval Midgut
Larval Hindgut
Larval Malpighian Tubules
Larval Fat Body
Larval Salivary Gland
Larval Trachea
Larval Carcass
Adult Head
Adult Eye
Adult Brain
Adult Thoracic-Abdominal Ganglion
Adult Crop
Adult Midgut
Adult Hindgut
Adult Malpighian Tubules
Adult Fat Body
Adult Salivary Gland
Adult Heart
Adult VirginFemale Spermatheca
Adult InseminatedFemale Spermatheca
Adult Ovary
Adult Testis
Adult Male Accessory Gland
Adult Carcass

FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)

modENCODE Anatomy RNA-Seq

modENCODE Tissue Expression Data

(modENCODE_mRNA-Seq_tissues)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		120
central nervous system, larvae L3		262
central nervous system, pupae P8		63
head, virgin 1-day female		3
head, virgin 4-day female		1
head, virgin 20-day female		1
head, mated 1-day female		3
head, mated 4-day female		2
head, mated 20-day female		3
head, mated 1-day male		3
head, mated 4-day male		4
head, mated 20-day male		2
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		22
digestive system, larvae L3 wandering		4
digestive system, 1-day adult		0
digestive system, 4-day adult		0
digestive system, 20-day adult		0
fat body, larvae L3 wandering		1
fat body, white prepupae		83
fat body, pupae P8		46
carcass, larvae L3 wandering		185
carcass, 1-day adult		1
carcass, 4-day adult		1
carcass, 20-day adult		1
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		0
accessory gland, mated 4-day male		5
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		(120)
central nervous system, larvae L3		(262)
central nervous system, pupae P8		(63)
head, virgin 1-day female		3
head, virgin 4-day female		1
head, virgin 20-day female		1
head, mated 1-day female		3
head, mated 4-day female		2
head, mated 20-day female		3
head, mated 1-day male		3
head, mated 4-day male		4
head, mated 20-day male		2
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		22
digestive system, larvae L3 wandering		4
digestive system, 1-day adult		0
digestive system, 4-day adult		0
digestive system, 20-day adult		0
fat body, larvae L3 wandering		1
fat body, white prepupae		(83)
fat body, pupae P8		(46)
carcass, larvae L3 wandering		(185)
carcass, 1-day adult		1
carcass, 4-day adult		1
carcass, 20-day adult		1
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		0
accessory gland, mated 4-day male		5
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		(120)
central nervous system, larvae L3		(262)
central nervous system, pupae P8		63
head, virgin 1-day female		3
head, virgin 4-day female		1
head, virgin 20-day female		1
head, mated 1-day female		3
head, mated 4-day female		2
head, mated 20-day female		3
head, mated 1-day male		3
head, mated 4-day male		4
head, mated 20-day male		2
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		22
digestive system, larvae L3 wandering		4
digestive system, 1-day adult		0
digestive system, 4-day adult		0
digestive system, 20-day adult		0
fat body, larvae L3 wandering		1
fat body, white prepupae		83
fat body, pupae P8		46
carcass, larvae L3 wandering		(185)
carcass, 1-day adult		1
carcass, 4-day adult		1
carcass, 20-day adult		1
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		0
accessory gland, mated 4-day male		5
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		120
central nervous system, larvae L3		262
central nervous system, pupae P8		63
head, virgin 1-day female		3
head, virgin 4-day female		1
head, virgin 20-day female		1
head, mated 1-day female		3
head, mated 4-day female		2
head, mated 20-day female		3
head, mated 1-day male		3
head, mated 4-day male		4
head, mated 20-day male		2
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		22
digestive system, larvae L3 wandering		4
digestive system, 1-day adult		0
digestive system, 4-day adult		0
digestive system, 20-day adult		0
fat body, larvae L3 wandering		1
fat body, white prepupae		83
fat body, pupae P8		46
carcass, larvae L3 wandering		185
carcass, 1-day adult		1
carcass, 4-day adult		1
carcass, 20-day adult		1
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		0
accessory gland, mated 4-day male		5
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		120
central nervous system, larvae L3		262
central nervous system, pupae P8		63
head, virgin 1-day female		3
head, virgin 4-day female		1
head, virgin 20-day female		1
head, mated 1-day female		3
head, mated 4-day female		2
head, mated 20-day female		3
head, mated 1-day male		3
head, mated 4-day male		4
head, mated 20-day male		2
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		22
digestive system, larvae L3 wandering		4
digestive system, 1-day adult		0
digestive system, 4-day adult		0
digestive system, 20-day adult		0
fat body, larvae L3 wandering		1
fat body, white prepupae		83
fat body, pupae P8		46
carcass, larvae L3 wandering		185
carcass, 1-day adult		1
carcass, 4-day adult		1
carcass, 20-day adult		1
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		0
accessory gland, mated 4-day male		5
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		(120)
central nervous system, larvae L3		(262)
central nervous system, pupae P8		(63)
head, virgin 1-day female		3
head, virgin 4-day female		1
head, virgin 20-day female		1
head, mated 1-day female		3
head, mated 4-day female		2
head, mated 20-day female		3
head, mated 1-day male		3
head, mated 4-day male		4
head, mated 20-day male		2
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		22
digestive system, larvae L3 wandering		4
digestive system, 1-day adult		0
digestive system, 4-day adult		0
digestive system, 20-day adult		0
fat body, larvae L3 wandering		1
fat body, white prepupae		(83)
fat body, pupae P8		(46)
carcass, larvae L3 wandering		(185)
carcass, 1-day adult		1
carcass, 4-day adult		1
carcass, 20-day adult		1
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		0
accessory gland, mated 4-day male		5
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		120
central nervous system, larvae L3		(262)
central nervous system, pupae P8		63
head, virgin 1-day female		3
head, virgin 4-day female		1
head, virgin 20-day female		1
head, mated 1-day female		3
head, mated 4-day female		2
head, mated 20-day female		3
head, mated 1-day male		3
head, mated 4-day male		4
head, mated 20-day male		2
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		22
digestive system, larvae L3 wandering		4
digestive system, 1-day adult		0
digestive system, 4-day adult		0
digestive system, 20-day adult		0
fat body, larvae L3 wandering		1
fat body, white prepupae		83
fat body, pupae P8		46
carcass, larvae L3 wandering		(185)
carcass, 1-day adult		1
carcass, 4-day adult		1
carcass, 20-day adult		1
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		0
accessory gland, mated 4-day male		5
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		120
central nervous system, larvae L3		262
central nervous system, pupae P8		63
head, virgin 1-day female		3
head, virgin 4-day female		1
head, virgin 20-day female		1
head, mated 1-day female		3
head, mated 4-day female		2
head, mated 20-day female		3
head, mated 1-day male		3
head, mated 4-day male		4
head, mated 20-day male		2
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		22
digestive system, larvae L3 wandering		4
digestive system, 1-day adult		0
digestive system, 4-day adult		0
digestive system, 20-day adult		0
fat body, larvae L3 wandering		1
fat body, white prepupae		83
fat body, pupae P8		46
carcass, larvae L3 wandering		185
carcass, 1-day adult		1
carcass, 4-day adult		1
carcass, 20-day adult		1
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		0
accessory gland, mated 4-day male		5
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Tissue		Expression Level
imaginal disc, larvae L3 wandering
central nervous system, larvae L3
central nervous system, pupae P8
head, virgin 1-day female
head, virgin 4-day female
head, virgin 20-day female
head, mated 1-day female
head, mated 4-day female
head, mated 20-day female
head, mated 1-day male
head, mated 4-day male
head, mated 20-day male
salivary gland, larvae L3 wandering
salivary gland, white prepupae
digestive system, larvae L3 wandering
digestive system, 1-day adult
digestive system, 4-day adult
digestive system, 20-day adult
fat body, larvae L3 wandering
fat body, white prepupae
fat body, pupae P8
carcass, larvae L3 wandering
carcass, 1-day adult
carcass, 4-day adult
carcass, 20-day adult
ovary, virgin 4-day female
ovary, mated 4-day female
testis, mated 4-day male
accessory gland, mated 4-day male

modENCODE Development RNA-Seq

modENCODE Temporal Expression Data

(modENCODE_mRNA-Seq_U)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of extremely low expression. Peak expression observed within 12-18 embryonic stages.
[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		1
embryo 06-08hr		4
embryo 08-10hr		7
embryo 10-12hr		17
embryo 12-14hr		69
embryo 14-16hr		168
embryo 16-18hr		27
embryo 18-20hr		5
embryo 20-22hr		2
embryo 22-24hr		2
larva L1		3
larva L2		76
larva L3 12hr old		13
larva L3 puffstage 1-2		2
larva L3 puffstage 3-6		15
larva L3 puffstage 7-9		43
white prepupae new		81
white prepupae 12hr		15
white prepupae 24hr		22
pupae 2d postWPP		118
pupae 3d postWPP		31
pupae 4d postWPP		1
adult male 01day		0
adult male 05day		0
adult male 30day		0
adult female 01day		0
adult female 05day		0
adult female 30day		0
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		1
embryo 06-08hr		4
embryo 08-10hr		7
embryo 10-12hr		17
embryo 12-14hr		(69)
embryo 14-16hr		(168)
embryo 16-18hr		(27)
embryo 18-20hr		5
embryo 20-22hr		2
embryo 22-24hr		2
larva L1		3
larva L2		(76)
larva L3 12hr old		13
larva L3 puffstage 1-2		2
larva L3 puffstage 3-6		15
larva L3 puffstage 7-9		(43)
white prepupae new		(81)
white prepupae 12hr		15
white prepupae 24hr		22
pupae 2d postWPP		(118)
pupae 3d postWPP		(31)
pupae 4d postWPP		1
adult male 01day		0
adult male 05day		0
adult male 30day		0
adult female 01day		0
adult female 05day		0
adult female 30day		0
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		1
embryo 06-08hr		4
embryo 08-10hr		7
embryo 10-12hr		17
embryo 12-14hr		69
embryo 14-16hr		(168)
embryo 16-18hr		27
embryo 18-20hr		5
embryo 20-22hr		2
embryo 22-24hr		2
larva L1		3
larva L2		76
larva L3 12hr old		13
larva L3 puffstage 1-2		2
larva L3 puffstage 3-6		15
larva L3 puffstage 7-9		43
white prepupae new		81
white prepupae 12hr		15
white prepupae 24hr		22
pupae 2d postWPP		(118)
pupae 3d postWPP		31
pupae 4d postWPP		1
adult male 01day		0
adult male 05day		0
adult male 30day		0
adult female 01day		0
adult female 05day		0
adult female 30day		0
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		1
embryo 06-08hr		4
embryo 08-10hr		7
embryo 10-12hr		17
embryo 12-14hr		69
embryo 14-16hr		168
embryo 16-18hr		27
embryo 18-20hr		5
embryo 20-22hr		2
embryo 22-24hr		2
larva L1		3
larva L2		76
larva L3 12hr old		13
larva L3 puffstage 1-2		2
larva L3 puffstage 3-6		15
larva L3 puffstage 7-9		43
white prepupae new		81
white prepupae 12hr		15
white prepupae 24hr		22
pupae 2d postWPP		118
pupae 3d postWPP		31
pupae 4d postWPP		1
adult male 01day		0
adult male 05day		0
adult male 30day		0
adult female 01day		0
adult female 05day		0
adult female 30day		0
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		1
embryo 06-08hr		4
embryo 08-10hr		7
embryo 10-12hr		17
embryo 12-14hr		69
embryo 14-16hr		168
embryo 16-18hr		27
embryo 18-20hr		5
embryo 20-22hr		2
embryo 22-24hr		2
larva L1		3
larva L2		76
larva L3 12hr old		13
larva L3 puffstage 1-2		2
larva L3 puffstage 3-6		15
larva L3 puffstage 7-9		43
white prepupae new		81
white prepupae 12hr		15
white prepupae 24hr		22
pupae 2d postWPP		118
pupae 3d postWPP		31
pupae 4d postWPP		1
adult male 01day		0
adult male 05day		0
adult male 30day		0
adult female 01day		0
adult female 05day		0
adult female 30day		0
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		1
embryo 06-08hr		4
embryo 08-10hr		7
embryo 10-12hr		17
embryo 12-14hr		(69)
embryo 14-16hr		(168)
embryo 16-18hr		27
embryo 18-20hr		5
embryo 20-22hr		2
embryo 22-24hr		2
larva L1		3
larva L2		(76)
larva L3 12hr old		13
larva L3 puffstage 1-2		2
larva L3 puffstage 3-6		15
larva L3 puffstage 7-9		(43)
white prepupae new		(81)
white prepupae 12hr		15
white prepupae 24hr		22
pupae 2d postWPP		(118)
pupae 3d postWPP		31
pupae 4d postWPP		1
adult male 01day		0
adult male 05day		0
adult male 30day		0
adult female 01day		0
adult female 05day		0
adult female 30day		0
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		1
embryo 06-08hr		4
embryo 08-10hr		7
embryo 10-12hr		17
embryo 12-14hr		69
embryo 14-16hr		(168)
embryo 16-18hr		27
embryo 18-20hr		5
embryo 20-22hr		2
embryo 22-24hr		2
larva L1		3
larva L2		76
larva L3 12hr old		13
larva L3 puffstage 1-2		2
larva L3 puffstage 3-6		15
larva L3 puffstage 7-9		43
white prepupae new		81
white prepupae 12hr		15
white prepupae 24hr		22
pupae 2d postWPP		118
pupae 3d postWPP		31
pupae 4d postWPP		1
adult male 01day		0
adult male 05day		0
adult male 30day		0
adult female 01day		0
adult female 05day		0
adult female 30day		0
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		1
embryo 06-08hr		4
embryo 08-10hr		7
embryo 10-12hr		17
embryo 12-14hr		69
embryo 14-16hr		168
embryo 16-18hr		27
embryo 18-20hr		5
embryo 20-22hr		2
embryo 22-24hr		2
larva L1		3
larva L2		76
larva L3 12hr old		13
larva L3 puffstage 1-2		2
larva L3 puffstage 3-6		15
larva L3 puffstage 7-9		43
white prepupae new		81
white prepupae 12hr		15
white prepupae 24hr		22
pupae 2d postWPP		118
pupae 3d postWPP		31
pupae 4d postWPP		1
adult male 01day		0
adult male 05day		0
adult male 30day		0
adult female 01day		0
adult female 05day		0
adult female 30day		0
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Developmental Stage		Expression Level
embryo 00-02hr
embryo 02-04hr
embryo 04-06hr
embryo 06-08hr
embryo 08-10hr
embryo 10-12hr
embryo 12-14hr
embryo 14-16hr
embryo 16-18hr
embryo 18-20hr
embryo 20-22hr
embryo 22-24hr
larva L1
larva L2
larva L3 12hr old
larva L3 puffstage 1-2
larva L3 puffstage 3-6
larva L3 puffstage 7-9
white prepupae new
white prepupae 12hr
white prepupae 24hr
pupae 2d postWPP
pupae 3d postWPP
pupae 4d postWPP
adult male 01day
adult male 05day
adult male 30day
adult female 01day
adult female 05day
adult female 30day

modENCODE Temporal Expression Data (Graveley et al., 2011)

modENCODE Cell Lines RNA-Seq

modENCODE Cell Line Expression Data

(modENCODE_mRNA-Seq_cell.A)

(modENCODE_mRNA-Seq_cell.B)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		0
Schneider line 2 Sg4		0
embryonic 1182-4H		0
embryonic GM2		0
embryonic Kc167		0
embryonic S1		0
embryonic S3		0
leg disc CME L1		0
wing disc CME-W2		0
wing disc ML-DmD8		0
wing disc ML-DmD9		0
wing disc ML-DmD16-c3		0
wing disc ML-DmD21		0
wing disc ML-DmD32		0
haltere disc ML-DmD17-c3		0
eye-antennal disc ML-DmD11		0
antennal disc ML-DmD20-c5		0
mixed discs ML-DmD4-c1		0
CNS ML-DmBG1-c1		0
CNS ML-DmBG2-c2		0
tumorous blood cells mbn2		0
ovary fGS/OSS		0
ovary OSC		0
ovary OSS		2
Expression Level Scale		Very low Low Moderate

Linear, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		0
Schneider line 2 Sg4		0
embryonic 1182-4H		0
embryonic GM2		0
embryonic Kc167		0
embryonic S1		0
embryonic S3		0
leg disc CME L1		0
wing disc CME-W2		0
wing disc ML-DmD8		0
wing disc ML-DmD9		0
wing disc ML-DmD16-c3		0
wing disc ML-DmD21		0
wing disc ML-DmD32		0
haltere disc ML-DmD17-c3		0
eye-antennal disc ML-DmD11		0
antennal disc ML-DmD20-c5		0
mixed discs ML-DmD4-c1		0
CNS ML-DmBG1-c1		0
CNS ML-DmBG2-c2		0
tumorous blood cells mbn2		0
ovary fGS/OSS		0
ovary OSC		0
ovary OSS		2
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		0
Schneider line 2 Sg4		0
embryonic 1182-4H		0
embryonic GM2		0
embryonic Kc167		0
embryonic S1		0
embryonic S3		0
leg disc CME L1		0
wing disc CME-W2		0
wing disc ML-DmD8		0
wing disc ML-DmD9		0
wing disc ML-DmD16-c3		0
wing disc ML-DmD21		0
wing disc ML-DmD32		0
haltere disc ML-DmD17-c3		0
eye-antennal disc ML-DmD11		0
antennal disc ML-DmD20-c5		0
mixed discs ML-DmD4-c1		0
CNS ML-DmBG1-c1		0
CNS ML-DmBG2-c2		0
tumorous blood cells mbn2		0
ovary fGS/OSS		0
ovary OSC		0
ovary OSS		2
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		0
Schneider line 2 Sg4		0
embryonic 1182-4H		0
embryonic GM2		0
embryonic Kc167		0
embryonic S1		0
embryonic S3		0
leg disc CME L1		0
wing disc CME-W2		0
wing disc ML-DmD8		0
wing disc ML-DmD9		0
wing disc ML-DmD16-c3		0
wing disc ML-DmD21		0
wing disc ML-DmD32		0
haltere disc ML-DmD17-c3		0
eye-antennal disc ML-DmD11		0
antennal disc ML-DmD20-c5		0
mixed discs ML-DmD4-c1		0
CNS ML-DmBG1-c1		0
CNS ML-DmBG2-c2		0
tumorous blood cells mbn2		0
ovary fGS/OSS		0
ovary OSC		0
ovary OSS		2
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		0
Schneider line 2 Sg4		0
embryonic 1182-4H		0
embryonic GM2		0
embryonic Kc167		0
embryonic S1		0
embryonic S3		0
leg disc CME L1		0
wing disc CME-W2		0
wing disc ML-DmD8		0
wing disc ML-DmD9		0
wing disc ML-DmD16-c3		0
wing disc ML-DmD21		0
wing disc ML-DmD32		0
haltere disc ML-DmD17-c3		0
eye-antennal disc ML-DmD11		0
antennal disc ML-DmD20-c5		0
mixed discs ML-DmD4-c1		0
CNS ML-DmBG1-c1		0
CNS ML-DmBG2-c2		0
tumorous blood cells mbn2		0
ovary fGS/OSS		0
ovary OSC		0
ovary OSS		2
Expression Level Scale		Very low Low Moderate

log, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		0
Schneider line 2 Sg4		0
embryonic 1182-4H		0
embryonic GM2		0
embryonic Kc167		0
embryonic S1		0
embryonic S3		0
leg disc CME L1		0
wing disc CME-W2		0
wing disc ML-DmD8		0
wing disc ML-DmD9		0
wing disc ML-DmD16-c3		0
wing disc ML-DmD21		0
wing disc ML-DmD32		0
haltere disc ML-DmD17-c3		0
eye-antennal disc ML-DmD11		0
antennal disc ML-DmD20-c5		0
mixed discs ML-DmD4-c1		0
CNS ML-DmBG1-c1		0
CNS ML-DmBG2-c2		0
tumorous blood cells mbn2		0
ovary fGS/OSS		0
ovary OSC		0
ovary OSS		2
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		0
Schneider line 2 Sg4		0
embryonic 1182-4H		0
embryonic GM2		0
embryonic Kc167		0
embryonic S1		0
embryonic S3		0
leg disc CME L1		0
wing disc CME-W2		0
wing disc ML-DmD8		0
wing disc ML-DmD9		0
wing disc ML-DmD16-c3		0
wing disc ML-DmD21		0
wing disc ML-DmD32		0
haltere disc ML-DmD17-c3		0
eye-antennal disc ML-DmD11		0
antennal disc ML-DmD20-c5		0
mixed discs ML-DmD4-c1		0
CNS ML-DmBG1-c1		0
CNS ML-DmBG2-c2		0
tumorous blood cells mbn2		0
ovary fGS/OSS		0
ovary OSC		0
ovary OSS		2
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		0
Schneider line 2 Sg4		0
embryonic 1182-4H		0
embryonic GM2		0
embryonic Kc167		0
embryonic S1		0
embryonic S3		0
leg disc CME L1		0
wing disc CME-W2		0
wing disc ML-DmD8		0
wing disc ML-DmD9		0
wing disc ML-DmD16-c3		0
wing disc ML-DmD21		0
wing disc ML-DmD32		0
haltere disc ML-DmD17-c3		0
eye-antennal disc ML-DmD11		0
antennal disc ML-DmD20-c5		0
mixed discs ML-DmD4-c1		0
CNS ML-DmBG1-c1		0
CNS ML-DmBG2-c2		0
tumorous blood cells mbn2		0
ovary fGS/OSS		0
ovary OSC		0
ovary OSS		2
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Cell Line		Expression Level
Schneider line 2 S2R+
Schneider line 2 Sg4
embryonic 1182-4H
embryonic GM2
embryonic Kc167
embryonic S1
embryonic S3
leg disc CME L1
wing disc CME-W2
wing disc ML-DmD8
wing disc ML-DmD9
wing disc ML-DmD16-c3
wing disc ML-DmD21
wing disc ML-DmD32
haltere disc ML-DmD17-c3
eye-antennal disc ML-DmD11
antennal disc ML-DmD20-c5
mixed discs ML-DmD4-c1
CNS ML-DmBG1-c1
CNS ML-DmBG2-c2
tumorous blood cells mbn2
ovary fGS/OSS
ovary OSC
ovary OSS

modENCODE Treatments RNA-Seq

modENCODE Treatment Expression Data

(modENCODE_mRNA-Seq_treatments)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		45
cold shock, 4-day adult		50
heat shock, 4-day adult		1
Cadmium 50 mM 6 hrs, larvae L3		2
Cadmium 50 mM 12 hrs, larvae L3		9
Cadmium 50 mM 48 hrs, 4-day adult		2
Cadmium 100 mM 48 hrs, 4-day adult		1
Copper 0.5 mM 12 hrs, larvae L3		7
Copper 15 mM 48 hrs, 4-day adult		6
Zinc 5 mM 12 hrs, larvae L3		18
Zinc 4.5 mM 48 hrs, 4-day adult		10
Ethanol 2.5% 3 hrs, larvae L3		1
Ethanol 5% 3 hrs, larvae L3		1
Ethanol 10% 3 hrs, larvae L3		2
Caffeine 1.5 mg/ml 4 hrs, larvae L3		1
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		2
Caffeine 25 mg/ml 48 hrs, 4-day adult		31
Paraquat 5 mM 48 hrs, 4-day adult		1
Paraquat 10 mM 48 hrs, 4-day adult		3
Rotenone 2 μg 12 hrs, larvae L3		1
Rotenone 8 μg 12 hrs, larvae L3		1
Expression Level Scale		Very low Low Moderate Moderately high High

Linear, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		(45)
cold shock, 4-day adult		(50)
heat shock, 4-day adult		1
Cadmium 50 mM 6 hrs, larvae L3		2
Cadmium 50 mM 12 hrs, larvae L3		9
Cadmium 50 mM 48 hrs, 4-day adult		2
Cadmium 100 mM 48 hrs, 4-day adult		1
Copper 0.5 mM 12 hrs, larvae L3		7
Copper 15 mM 48 hrs, 4-day adult		6
Zinc 5 mM 12 hrs, larvae L3		18
Zinc 4.5 mM 48 hrs, 4-day adult		10
Ethanol 2.5% 3 hrs, larvae L3		1
Ethanol 5% 3 hrs, larvae L3		1
Ethanol 10% 3 hrs, larvae L3		2
Caffeine 1.5 mg/ml 4 hrs, larvae L3		1
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		2
Caffeine 25 mg/ml 48 hrs, 4-day adult		(31)
Paraquat 5 mM 48 hrs, 4-day adult		1
Paraquat 10 mM 48 hrs, 4-day adult		3
Rotenone 2 μg 12 hrs, larvae L3		1
Rotenone 8 μg 12 hrs, larvae L3		1
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		45
cold shock, 4-day adult		50
heat shock, 4-day adult		1
Cadmium 50 mM 6 hrs, larvae L3		2
Cadmium 50 mM 12 hrs, larvae L3		9
Cadmium 50 mM 48 hrs, 4-day adult		2
Cadmium 100 mM 48 hrs, 4-day adult		1
Copper 0.5 mM 12 hrs, larvae L3		7
Copper 15 mM 48 hrs, 4-day adult		6
Zinc 5 mM 12 hrs, larvae L3		18
Zinc 4.5 mM 48 hrs, 4-day adult		10
Ethanol 2.5% 3 hrs, larvae L3		1
Ethanol 5% 3 hrs, larvae L3		1
Ethanol 10% 3 hrs, larvae L3		2
Caffeine 1.5 mg/ml 4 hrs, larvae L3		1
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		2
Caffeine 25 mg/ml 48 hrs, 4-day adult		31
Paraquat 5 mM 48 hrs, 4-day adult		1
Paraquat 10 mM 48 hrs, 4-day adult		3
Rotenone 2 μg 12 hrs, larvae L3		1
Rotenone 8 μg 12 hrs, larvae L3		1
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		45
cold shock, 4-day adult		50
heat shock, 4-day adult		1
Cadmium 50 mM 6 hrs, larvae L3		2
Cadmium 50 mM 12 hrs, larvae L3		9
Cadmium 50 mM 48 hrs, 4-day adult		2
Cadmium 100 mM 48 hrs, 4-day adult		1
Copper 0.5 mM 12 hrs, larvae L3		7
Copper 15 mM 48 hrs, 4-day adult		6
Zinc 5 mM 12 hrs, larvae L3		18
Zinc 4.5 mM 48 hrs, 4-day adult		10
Ethanol 2.5% 3 hrs, larvae L3		1
Ethanol 5% 3 hrs, larvae L3		1
Ethanol 10% 3 hrs, larvae L3		2
Caffeine 1.5 mg/ml 4 hrs, larvae L3		1
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		2
Caffeine 25 mg/ml 48 hrs, 4-day adult		31
Paraquat 5 mM 48 hrs, 4-day adult		1
Paraquat 10 mM 48 hrs, 4-day adult		3
Rotenone 2 μg 12 hrs, larvae L3		1
Rotenone 8 μg 12 hrs, larvae L3		1
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		45
cold shock, 4-day adult		50
heat shock, 4-day adult		1
Cadmium 50 mM 6 hrs, larvae L3		2
Cadmium 50 mM 12 hrs, larvae L3		9
Cadmium 50 mM 48 hrs, 4-day adult		2
Cadmium 100 mM 48 hrs, 4-day adult		1
Copper 0.5 mM 12 hrs, larvae L3		7
Copper 15 mM 48 hrs, 4-day adult		6
Zinc 5 mM 12 hrs, larvae L3		18
Zinc 4.5 mM 48 hrs, 4-day adult		10
Ethanol 2.5% 3 hrs, larvae L3		1
Ethanol 5% 3 hrs, larvae L3		1
Ethanol 10% 3 hrs, larvae L3		2
Caffeine 1.5 mg/ml 4 hrs, larvae L3		1
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		2
Caffeine 25 mg/ml 48 hrs, 4-day adult		31
Paraquat 5 mM 48 hrs, 4-day adult		1
Paraquat 10 mM 48 hrs, 4-day adult		3
Rotenone 2 μg 12 hrs, larvae L3		1
Rotenone 8 μg 12 hrs, larvae L3		1
Expression Level Scale		Very low Low Moderate Moderately high High

log, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		(45)
cold shock, 4-day adult		(50)
heat shock, 4-day adult		1
Cadmium 50 mM 6 hrs, larvae L3		2
Cadmium 50 mM 12 hrs, larvae L3		9
Cadmium 50 mM 48 hrs, 4-day adult		2
Cadmium 100 mM 48 hrs, 4-day adult		1
Copper 0.5 mM 12 hrs, larvae L3		7
Copper 15 mM 48 hrs, 4-day adult		6
Zinc 5 mM 12 hrs, larvae L3		18
Zinc 4.5 mM 48 hrs, 4-day adult		10
Ethanol 2.5% 3 hrs, larvae L3		1
Ethanol 5% 3 hrs, larvae L3		1
Ethanol 10% 3 hrs, larvae L3		2
Caffeine 1.5 mg/ml 4 hrs, larvae L3		1
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		2
Caffeine 25 mg/ml 48 hrs, 4-day adult		31
Paraquat 5 mM 48 hrs, 4-day adult		1
Paraquat 10 mM 48 hrs, 4-day adult		3
Rotenone 2 μg 12 hrs, larvae L3		1
Rotenone 8 μg 12 hrs, larvae L3		1
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		45
cold shock, 4-day adult		50
heat shock, 4-day adult		1
Cadmium 50 mM 6 hrs, larvae L3		2
Cadmium 50 mM 12 hrs, larvae L3		9
Cadmium 50 mM 48 hrs, 4-day adult		2
Cadmium 100 mM 48 hrs, 4-day adult		1
Copper 0.5 mM 12 hrs, larvae L3		7
Copper 15 mM 48 hrs, 4-day adult		6
Zinc 5 mM 12 hrs, larvae L3		18
Zinc 4.5 mM 48 hrs, 4-day adult		10
Ethanol 2.5% 3 hrs, larvae L3		1
Ethanol 5% 3 hrs, larvae L3		1
Ethanol 10% 3 hrs, larvae L3		2
Caffeine 1.5 mg/ml 4 hrs, larvae L3		1
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		2
Caffeine 25 mg/ml 48 hrs, 4-day adult		31
Paraquat 5 mM 48 hrs, 4-day adult		1
Paraquat 10 mM 48 hrs, 4-day adult		3
Rotenone 2 μg 12 hrs, larvae L3		1
Rotenone 8 μg 12 hrs, larvae L3		1
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		45
cold shock, 4-day adult		50
heat shock, 4-day adult		1
Cadmium 50 mM 6 hrs, larvae L3		2
Cadmium 50 mM 12 hrs, larvae L3		9
Cadmium 50 mM 48 hrs, 4-day adult		2
Cadmium 100 mM 48 hrs, 4-day adult		1
Copper 0.5 mM 12 hrs, larvae L3		7
Copper 15 mM 48 hrs, 4-day adult		6
Zinc 5 mM 12 hrs, larvae L3		18
Zinc 4.5 mM 48 hrs, 4-day adult		10
Ethanol 2.5% 3 hrs, larvae L3		1
Ethanol 5% 3 hrs, larvae L3		1
Ethanol 10% 3 hrs, larvae L3		2
Caffeine 1.5 mg/ml 4 hrs, larvae L3		1
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		2
Caffeine 25 mg/ml 48 hrs, 4-day adult		31
Paraquat 5 mM 48 hrs, 4-day adult		1
Paraquat 10 mM 48 hrs, 4-day adult		3
Rotenone 2 μg 12 hrs, larvae L3		1
Rotenone 8 μg 12 hrs, larvae L3		1
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Treatment		Expression Level
extended cold, 4-day adult
cold shock, 4-day adult
heat shock, 4-day adult
Cadmium 50 mM 6 hrs, larvae L3
Cadmium 50 mM 12 hrs, larvae L3
Cadmium 50 mM 48 hrs, 4-day adult
Cadmium 100 mM 48 hrs, 4-day adult
Copper 0.5 mM 12 hrs, larvae L3
Copper 15 mM 48 hrs, 4-day adult
Zinc 5 mM 12 hrs, larvae L3
Zinc 4.5 mM 48 hrs, 4-day adult
Ethanol 2.5% 3 hrs, larvae L3
Ethanol 5% 3 hrs, larvae L3
Ethanol 10% 3 hrs, larvae L3
Caffeine 1.5 mg/ml 4 hrs, larvae L3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
Caffeine 25 mg/ml 48 hrs, 4-day adult
Paraquat 5 mM 48 hrs, 4-day adult
Paraquat 10 mM 48 hrs, 4-day adult
Rotenone 2 μg 12 hrs, larvae L3
Rotenone 8 μg 12 hrs, larvae L3

Expression Clusters

A cluster of genes with similar mRNA expression dynamics across development.

(The modENCODE Consortium, 2010)

mE2_34_mRNA_expression_cluster_07

External Data & Images

Linkouts

BDGP expression data

•

RE39082

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0001253

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG32356-RA;CG32356-RB;HDC09080;CG32356-RB

FlyExpress - Embryonic expression images (BDGP data)

•

Show all FlyExpress processed images for ImpE1

Stages(s) 11-12

Stages(s) 13-16

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

lethal, with Scer\GAL4^Act5C.PU

ImpE1^KK111088

viable

ImpE1^f03112

viable, with Scer\GAL4^pnr-MD237

ImpE1^GD4812

Sterility

Allele

fertile

ImpE1^f03112

Phenotype manifest in

Allele

Classical Alleles ( 2 )

For All Classical Alleles Show

Allele of ImpE1	Class	Mutagen	Stocks	Known lesion
ImpE1^f03112		piggyBac transposase	2	--
ImpE1^MB01017			1	--

Alleles Carried on Transgenic Constructs ( 3 )

For All Alleles Carried on Transgenic Constructs Show

Allele of ImpE1	Mutagen	Stocks	Known lesion
ImpE1^GD4812	in vitro construct - RNAi in vitro construct - regulatory fusion	2	Yes
ImpE1^KK111088	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
ImpE1^NIG.32356R	in vitro construct - RNAi in vitro construct - regulatory fusion	0	Yes

Aneuploid Aberrations

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD4812}

P{KK111088}

P{NIG.32356R}

Insertions

Type of insertions

Name

Expression data

miscellaneous insertions

PBac{WH}ImpE1^f03112

insertion of enhancer trap binary system

Mi{ET1}ImpE1^MB01017

Gene Ontology: Function, Process & Cellular Component ( 2 unique terms )

There is no information supporting a molecular function term for this gene.

(Based on a review of available data on: 2010-12-09)

Terms Based on Experimental Evidence ( 0 terms )

Terms Based on Predictions or Assertions ( 2 terms )

Molecular Function ( 0 terms)

Biological Process

CV term

Evidence

References

imaginal disc eversion

traceable author statement

(Andres et al., 1993)

Cellular Component

CV term

Evidence

References

extrinsic to membrane

traceable author statement

(Andres et al., 1993)

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Interactions & Pathways

Summary of Physical Interactions

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

ImpE1 allele

Gene

References

External Data

Linkouts

BioGRID - A database of protein and genetic interactions

•

69948

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0001253

InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets.

•

45879

Orthologs

OrthoDB Orthologs (28) - based on analysis using Dmel annotation version 5.41

OrthoDB Ortholog Groups

OrthoDB orthology group searches

(Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288)

Drosophila inclusive ortholog search

EOG61VKP3

Dipteran inclusive ortholog search

EOG666VVS

Insect inclusive ortholog search

EOG6MKMBJ

Arthropod inclusive ortholog search

EOG670RZJ

Metazoa inclusive ortholog search

No orthologs identified

Orthologs in Drosophila Species (EOG61VKP3)

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA16846

Drosophila persimilis

Dper\GL24700

Drosophila willistoni

Dwil\GK17755

Drosophila virilis

Dvir\GJ12968

Drosophila mojavensis

Dmoj\GI12827

Drosophila grimshawi

Dgri\GH15060

Orthologs in non-Drosophila Dipterans (EOG666VVS)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL000742

Anopheles gambiae

Malaria mosquito

Agam\AGAP006069

Orthologs in non-Dipteran Insects (EOG6MKMBJ)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Apis mellifera

Western honey bee

Amel\GB43230

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG009305

Acromyrmex echinatior

Panamanian leafcutter ant

Aech\AECH11447

Atta cephalotes

Leafcutter ant

Acep\ACEP21798

Camponotus floridanus

Florida carpenter ant

Cflo\CFLO22102

Harpegnathos saltator

Jerdons jumping ant

Hsal\HSAL17916

Linepithema humile

Argentine ant

Lhum\LH10981

Solenopsis invicta

Red fire ant

Sinv\SINV19865

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI009696

Bombyx mori

Silkmoth

Bmor\BGIBMGA006908

Pediculus humanus

Human body louse

Phum\PHUM064480

Tribolium castaneum

Red flour beetle

Tcas\TC012794

Orthologs in non-Insect Arthropods (EOG670RZJ)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Daphnia pulex

Water flea

Dpux\ABLIM1

Ixodes scapularis

Deer tick

Isca\ISCW008354

Orthologs in non-Arthropod Metazoa (None identified)

No non-Arthropod Metazoa orthologies identified

Human Orthologs (0)

Gene

OMIM

HGNC

AAA Orthologs (11) based on analysis using Dmel annotation version 4.3

Organism

Gene

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA16846

Drosophila persimilis

Dper\GL24700

Drosophila willistoni

Dwil\GK17755

Drosophila virilis

Dvir\GJ12968

Drosophila mojavensis

Dmoj\GI12827

Drosophila grimshawi

Dgri\GH15060

Stocks & Reagents

Stocks Listed in FlyBase ( 6 )

Bloomington

18621

w¹¹¹⁸; PBac{WH}ImpE1^f03112

22965

y¹ w^67c23; Mi{ET1}ImpE1^MB01017

v104613

v12854

w¹¹¹⁸; P{GD4812}v12854

v12855

w¹¹¹⁸; P{GD4812}v12855

Genomic Clones ( 2 )

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones ( 56 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Linkouts

DRSC - Results from RNAi screens.

•

FBgn0001253

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

45879

Antibody Information

Other Information

Discoverer

Etymology

Identification

Relationship to Other Genes

Source for database identity of

Source for database merge of

Source for merge of: ImpE1 BcDNA:RE39082

(FlyBase, 1996-)

Source for merge of: ImpE1 CG7116 CG13668

(Natzle, 2000.5.4)

Additional comments

Annotations CG13668, CG7116 merged as CG32356 in release 3 of the genome annotation.

(FlyBase Genome Annotators, 2002-2003)

Source for merge of ImpE1 BcDNA:RE39082 was a shared cDNA (date:030728).

(FlyBase, 1996-)

Other Comments

ImpE1 is expressed in response to both ecdysone and pattern formation genes and may play a part in the morphogenetic events of embryogenesis and imaginal disc eversion.

(Clark et al., 1995)

Down regulation of ImpE1 transcription is altered in Eip74EF mutants.

(Fletcher and Thummel, 1995)

ImpE1 and Dfd have been examined for their positions relative to the Broad complex genes in the hormone-regulated pathway of CNS metamorphosis. Activity of any individual Broad complex subfunction is not required for ImpE1 induction.

(Restifo et al., 1995)

Ecdysteroid-regulated gene.

(Andres et al., 1993)

The sequential temporal expression of steroid hormone-responsive genes in imaginal discs may be important in organizing cellular mechanisms involved in morphogenesis of the epithelium.

(Natzle, 1993)

Gene encoding a transcript associated with membrane-bound polysomes in imaginal discs and expressed only in response to 20-hydroxyecdysone (20 HOE). Expression studied both in vitro and in vivo. One of the 'early' genes involved in elongation of appendage-forming regions of the imaginal discs by means of epithelial-cell rearrangements (Natzle, Fristrom and Fristrom, 1988).

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

RefSeq (Proteins)

•

DDBJ / EMBL / GenBank

•

Entrez Gene - A searchable database of RefSeq genes.

•

45879

UniProtKB/TrEMBL

•

Other Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

EB domain (IPR006149)

Low-density lipoprotein (LDL) receptor class A, conserved site (IPR023415)

Low-density lipoprotein (LDL) receptor class A repeat (IPR002172)

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

•

RE39082

Linkouts

BioGRID - A database of protein and genetic interactions

•

69948

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0001253

DRSC - Results from RNAi screens.

•

FBgn0001253

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0001253

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG32356-RA;CG32356-RB;HDC09080;CG32356-RB

FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans

•

FBgn0001253

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

45879

InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets.

•

45879

modMine - Data generated by the modENCODE project.

•

FBgn0001253

Synonyms & Secondary IDs ( 11 )

Reported As

Symbol Synonym

BcDNA:RE39082

CG7116

(Harris et al., 2003, Natzle, 2000.5.4, )

CG13668

(Natzle, 2000.5.4, )

CG32356

(Lebo et al., 2009, Keleman et al., 2009.8.5)

CT22003

(Hynes and Zhao, 2000)

CT33103

(Hynes and Zhao, 2000)

Imp-E1

(Bialecki et al., 2002, Bayer et al., 1996, Restifo et al., 1995, Fletcher and Thummel, 1995, Andres et al., 1993, Natzle, 1993, )

ImpE1

(Butchar et al., 2012, Curtis et al., 2007, Ou et al., 2011, Japanese National Institute of Genetics, 2012.5.21)

Name Synonym

Ecdysone-inducible gene E1

(Lebo et al., 2009)

Y1-like

(Hynes and Zhao, 2000)

Secondary FlyBase IDs

FBgn0035884
FBgn0035885
FBgn0052356
FBgn0062446

References ( 60 )

Generate a list of

All references relating to this gene ( 60 )

List References by type

Recent research papers ( 3 )

Butchar et al., 2012, Genetics 191(4): 1213--1226: New negative feedback regulators of egfr signaling in Drosophila. [FBrf0219175]
Chawla and Sokol, 2012, Development 139(10): 1788--1797: Hormonal activation of let-7-C microRNAs via EcR is required for adult Drosophila melanogaster morphology and function. [FBrf0218077]
Ou et al., 2011, PLoS Biol. 9(9): e1001160: Nuclear Receptor DHR4 Controls the Timing of Steroid Hormone Pulses During Drosophila Development. [FBrf0216318]

Show all research papers ...

Recent reviews (0)

All reviews listed in FlyBase were published before 2011

Show reviews ...