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Gene Dmel\ImpE2

General Information
Symbol	Dmel\ImpE2	Species	D. melanogaster
Name	Ecdysone-inducible gene E2	Annotation symbol	CG1934
Feature type	protein_coding_gene	FlyBase ID	FBgn0001254
Gene Model Status	Current	Stock availability	3 publicly available
Also Known As	IMP-E2
Genomic Location
Chromosome (arm)	3L	Recombination map
Cytogenetic map	63E2-63E2	Sequence location	3L:3,510,974..3,512,681 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Ecdysone-inducible gene E2 is referred to in FlyBase by the symbol Dmel\ImpE2 (CG1934, FBgn0001254). It is a protein_coding_gene from Drosophila melanogaster. Its molecular function is unknown. It is reported to be involved in the biological process: imaginal disc eversion. 4 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of extremely high expression to a trough of extremely low expression. Peak expression observed during early pupal stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval carcass. Comments on Affy2 ProbeSet: ProbeSet 1634928_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of ImpE2. Gene sequence location is 3L:3510974..3512681.
User Contributed Data
	Beta version, feedback welcome
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	Imp-E: Inducible membrane-bound polysomes-Early Name given to three genes encoding transcripts associated with membrane-bound polysomes in imaginal discs and expressed only in response to 20-hydroxyecdysone (20 HOE). Expression studied both in vitro and in vivo. Imp-E denotes three "early" genes involved in elongation of appendage-forming regions of the imaginal discs by means of epithelial-cell rearrangements (Natzle et al., 1988).
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 63E2-63E2   Limits computationally determined from genome sequence between P{PZ}kst01318 and P{PZ}Sc205634
Experimentally Determined Cytological Location
Cytogenetic map Notes References 63E-63E   (determined by in situ hybridisation)   (Paine-Saunders et al., 1990) 63E-63E   (determined by in situ hybridisation)   (Natzle et al., 1986)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\ImpE2 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.44 Gene model reviewed during 5.46
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) ImpE2-RA FBtr0073141  NM_057232   1708   466
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein ImpE2-PA   FBpp0073000   49.8   466   4.06     NP_476580   AAF47780
Additional Polypeptide Data & Comments
Reported size (kDa)	466 (aa); 75 (kD observed); 48 (kD predicted) (Paine-Saunders et al., 1990)
Comments
External Data
Linkouts
Crossreferences
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA540201   AE014296 AAF47780   AC004658   AI257040   AI259016   AI260380   AI260911   AI260919   AI293747   AI293793   AI294062   AI294577   AI295218   AI296311   AI296489   AW942536   AY061270 AAL28818   BI168897   BI357736   BI365555   BI366716   CK657592   CK658483   CK658594   CK658706   CK658767   CK659269   CK659970   CK660529   CK660703   CK660906   CK660979   CK661049   CK661344   CK661386   CK662098   CO184575   CO190581   CO195329   CO270974   CO271089   CO275136   CO277993   CO286661   CO288279   CO288771   CO294904   CO296438   CO308710   CO308768   CO345090   EC058052   EC080885   EC081546   EC085777   EC087027   EC198613   EC207641   EC209526   EC212941   EC220218   EC220236   EC226627   EC236203   EC240609   M55099 AAA63632
UniProtKB/Swiss-Prot	P29681
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 4 -- 6   anterior endoderm anlage   (Fisher et al., 2012) Comment:anlage in statu nascendi endoderm anlage   (Fisher et al., 2012) Comment:anlage in statu nascendi foregut anlage in statu nascendi   (Fisher et al., 2012) posterior ectoderm anlage   (Fisher et al., 2012) Comment:anlage in statu nascendi embryonic stage 7 -- 8   antennal anlage   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage central brain anlage   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage dorsal head epidermis anlage   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage visual anlage   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage anterior endoderm anlage   (Fisher et al., 2012) foregut anlage   (Fisher et al., 2012) posterior endoderm   (Fisher et al., 2012) embryonic stage 9 -- 10   anterior endoderm   (Fisher et al., 2012) foregut primordium   (Fisher et al., 2012) posterior endoderm   (Fisher et al., 2012) embryonic stage 11 -- 12   anterior midgut primordium   (Fisher et al., 2012) foregut primordium   (Fisher et al., 2012) posterior midgut primordium   (Fisher et al., 2012) embryonic stage 13 -- 16   embryonic dorsal epidermis   (Fisher et al., 2012) embryonic esophagus   (Fisher et al., 2012) embryonic foregut   (Fisher et al., 2012) embryonic head sensory system   (Fisher et al., 2012) embryonic salivary gland duct   (Fisher et al., 2012) embryonic stomatogastric nervous system   (Fisher et al., 2012) embryonic ventral epidermis   (Fisher et al., 2012) embryonic/larval corpus cardiacum   (Fisher et al., 2012) embryonic stage 15 -- 16   ectoderm   (Paine-Saunders et al., 1990) Comment:reference states 12-15 hr AEL prepupal stage P1 -- P3   embryonic/larval proventriculus   (Paine-Saunders et al., 1990) Comment:reference states 0-4 hr APF northern blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage -- pupal stage     (Paine-Saunders et al., 1990)
Additional Descriptive Data
	Northern blotting reveals that the highest levels of ImpE2 transcript are present in white prepupae and early (24hr) pupae. Moderate levels are seen in 12-18 hr embryos and in late pupae (>48 hr). Low levels of transcript are found in 3-12 hour embryos and in larvae, while no transcripts are detected in adults, or in 0-3 hour embryos. When third instar larval imaginal discs are cultured for 3, 6, 9 or 12 hr in the presence of 20-hydroxyecdysone (20-HE), the 1.7 kb ImpE2 transcript is detected, while no transcript is detected in discs cultured for the same length of time without 20-HE. Radioactive in situ hybridization shows that in 12-15 hour embryos, transcript localizes to the ectoderm. In 0-4 hour prepupae, part of the columnar epithelium and the peripodal epithelium of each imaginal disc, and the epithelium of the proventriculus, accumulates ImpE2 transcript. (Paine-Saunders et al., 1990)
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization Stage Tissue/Position (including subcellular localization) Reference embryonic stage 15 -- 16   ectoderm   (Paine-Saunders et al., 1990) Comment:reference states 12-15 hr AEL
Additional Descriptive Data
	After 12 hours of embryogenesis but prior to dorsal closure, the ImpE2 protein is localized cytoplasmically in a punctate pattern, whereas after dorsal closure, it is found in an extracellular layer adjacent to the apical ectodermal surface. Embryos between these stages show a combination of staining, with punctate cytoplasmic (possibly vesicular) staining posteriorly, and extracellular staining anteriorly. ImpE2 protein is likely secreted apically. E.M. immunolocalization to prepupal imaginal discs shows ImpE2 protein to be localized to vesicles at (and fusing to) the apical cytoplasm. Some staining is also seen at the cell surface and the extracellular matrix. (Paine-Saunders et al., 1990)
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
cytoplasm inferred from direct assay (Paine-Saunders et al., 1990) extracellular region inferred from direct assay (Paine-Saunders et al., 1990)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    6.25 Larval Midgut    8.9 Larval Hindgut    23.9 Larval Malpighian Tubules    8.6 Larval Fat Body    7.2 Larval Salivary Gland    17.6 Larval Trachea    6.8 Larval Carcass    178.025 Adult Head    6.9 Adult Eye    6.75 Adult Brain    2.6 Adult Thoracic-Abdominal Ganglion    3 Adult Crop    12.6 Adult Midgut    10.9 Adult Hindgut    9.2 Adult Malpighian Tubules    7.5 Adult Fat Body    8 Adult Salivary Gland    9.4 Adult Heart    3.425 Adult VirginFemale Spermatheca    5.2 Adult InseminatedFemale Spermatheca    6.7 Adult Ovary    2.7 Adult Testis    5 Adult Male Accessory Gland    11 Adult Carcass    8.2 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    6.25 Larval Midgut    8.9 Larval Hindgut    23.9 Larval Malpighian Tubules    8.6 Larval Fat Body    7.2 Larval Salivary Gland    17.6 Larval Trachea    6.8 Larval Carcass    178.025 Adult Head    6.9 Adult Eye    6.75 Adult Brain    2.6 Adult Thoracic-Abdominal Ganglion    3 Adult Crop    12.6 Adult Midgut    10.9 Adult Hindgut    9.2 Adult Malpighian Tubules    7.5 Adult Fat Body    8 Adult Salivary Gland    9.4 Adult Heart    3.425 Adult VirginFemale Spermatheca    5.2 Adult InseminatedFemale Spermatheca    6.7 Adult Ovary    2.7 Adult Testis    5 Adult Male Accessory Gland    11 Adult Carcass    8.2 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    6.25 Larval Midgut    8.9 Larval Hindgut    23.9 Larval Malpighian Tubules    8.6 Larval Fat Body    7.2 Larval Salivary Gland    17.6 Larval Trachea    6.8 Larval Carcass    178.025 Adult Head    6.9 Adult Eye    6.75 Adult Brain    2.6 Adult Thoracic-Abdominal Ganglion    3 Adult Crop    12.6 Adult Midgut    10.9 Adult Hindgut    9.2 Adult Malpighian Tubules    7.5 Adult Fat Body    8 Adult Salivary Gland    9.4 Adult Heart    3.425 Adult VirginFemale Spermatheca    5.2 Adult InseminatedFemale Spermatheca    6.7 Adult Ovary    2.7 Adult Testis    5 Adult Male Accessory Gland    11 Adult Carcass    8.2 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    6.25 Larval Midgut    8.9 Larval Hindgut    23.9 Larval Malpighian Tubules    8.6 Larval Fat Body    7.2 Larval Salivary Gland    17.6 Larval Trachea    6.8 Larval Carcass    178.025 Adult Head    6.9 Adult Eye    6.75 Adult Brain    2.6 Adult Thoracic-Abdominal Ganglion    3 Adult Crop    12.6 Adult Midgut    10.9 Adult Hindgut    9.2 Adult Malpighian Tubules    7.5 Adult Fat Body    8 Adult Salivary Gland    9.4 Adult Heart    3.425 Adult VirginFemale Spermatheca    5.2 Adult InseminatedFemale Spermatheca    6.7 Adult Ovary    2.7 Adult Testis    5 Adult Male Accessory Gland    11 Adult Carcass    8.2 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    6.25 Larval Midgut    8.9 Larval Hindgut    23.9 Larval Malpighian Tubules    8.6 Larval Fat Body    7.2 Larval Salivary Gland    17.6 Larval Trachea    6.8 Larval Carcass    178.025 Adult Head    6.9 Adult Eye    6.75 Adult Brain    2.6 Adult Thoracic-Abdominal Ganglion    3 Adult Crop    12.6 Adult Midgut    10.9 Adult Hindgut    9.2 Adult Malpighian Tubules    7.5 Adult Fat Body    8 Adult Salivary Gland    9.4 Adult Heart    3.425 Adult VirginFemale Spermatheca    5.2 Adult InseminatedFemale Spermatheca    6.7 Adult Ovary    2.7 Adult Testis    5 Adult Male Accessory Gland    11 Adult Carcass    8.2 Expression Level Scale  None   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    6.25 Larval Midgut    8.9 Larval Hindgut    23.9 Larval Malpighian Tubules    8.6 Larval Fat Body    7.2 Larval Salivary Gland    17.6 Larval Trachea    6.8 Larval Carcass    178.025 Adult Head    6.9 Adult Eye    6.75 Adult Brain    2.6 Adult Thoracic-Abdominal Ganglion    3 Adult Crop    12.6 Adult Midgut    10.9 Adult Hindgut    9.2 Adult Malpighian Tubules    7.5 Adult Fat Body    8 Adult Salivary Gland    9.4 Adult Heart    3.425 Adult VirginFemale Spermatheca    5.2 Adult InseminatedFemale Spermatheca    6.7 Adult Ovary    2.7 Adult Testis    5 Adult Male Accessory Gland    11 Adult Carcass    8.2 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    6.25 Larval Midgut    8.9 Larval Hindgut    23.9 Larval Malpighian Tubules    8.6 Larval Fat Body    7.2 Larval Salivary Gland    17.6 Larval Trachea    6.8 Larval Carcass    178.025 Adult Head    6.9 Adult Eye    6.75 Adult Brain    2.6 Adult Thoracic-Abdominal Ganglion    3 Adult Crop    12.6 Adult Midgut    10.9 Adult Hindgut    9.2 Adult Malpighian Tubules    7.5 Adult Fat Body    8 Adult Salivary Gland    9.4 Adult Heart    3.425 Adult VirginFemale Spermatheca    5.2 Adult InseminatedFemale Spermatheca    6.7 Adult Ovary    2.7 Adult Testis    5 Adult Male Accessory Gland    11 Adult Carcass    8.2 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    6.25 Larval Midgut    8.9 Larval Hindgut    23.9 Larval Malpighian Tubules    8.6 Larval Fat Body    7.2 Larval Salivary Gland    17.6 Larval Trachea    6.8 Larval Carcass    178.025 Adult Head    6.9 Adult Eye    6.75 Adult Brain    2.6 Adult Thoracic-Abdominal Ganglion    3 Adult Crop    12.6 Adult Midgut    10.9 Adult Hindgut    9.2 Adult Malpighian Tubules    7.5 Adult Fat Body    8 Adult Salivary Gland    9.4 Adult Heart    3.425 Adult VirginFemale Spermatheca    5.2 Adult InseminatedFemale Spermatheca    6.7 Adult Ovary    2.7 Adult Testis    5 Adult Male Accessory Gland    11 Adult Carcass    8.2 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    6.9   NA  Eye    6.75   NA  Brain    2.6   6.25  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    3   NA  Crop    no informative data   8.9  Midgut    no informative data   23.9  Hindgut    9.2   8.6  Malpighian Tubules    7.5   7.2  Fat Body    8   17.6  Salivary Gland    9.4   NA  Heart    3.425   6.8  Trachea    NA   NA  VirginFemale Spermatheca    5.2   NA  InseminatedFemale Spermatheca    6.7   NA  Ovary    2.7   NA  Testis    5   NA  Male Accessory Gland    no informative data   178.025  Carcass    8.2 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    1313 central nervous system, larvae L3    199 central nervous system, pupae P8    29 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    2 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    149 digestive system, larvae L3 wandering    29 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    2 fat body, larvae L3 wandering    0 fat body, white prepupae    279 fat body, pupae P8    117 carcass, larvae L3 wandering    796 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    7 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (1313) central nervous system, larvae L3  (199) central nervous system, pupae P8  (29) head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    2 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae  (149) digestive system, larvae L3 wandering  (29) digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    2 fat body, larvae L3 wandering    0 fat body, white prepupae  (279) fat body, pupae P8  (117) carcass, larvae L3 wandering  (796) carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    7 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (1313) central nervous system, larvae L3  (199) central nervous system, pupae P8    29 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    2 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae  (149) digestive system, larvae L3 wandering    29 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    2 fat body, larvae L3 wandering    0 fat body, white prepupae  (279) fat body, pupae P8  (117) carcass, larvae L3 wandering  (796) carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    7 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    1313 central nervous system, larvae L3    199 central nervous system, pupae P8    29 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    2 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    149 digestive system, larvae L3 wandering    29 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    2 fat body, larvae L3 wandering    0 fat body, white prepupae    279 fat body, pupae P8    117 carcass, larvae L3 wandering    796 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    7 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    4 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    1313 central nervous system, larvae L3    199 central nervous system, pupae P8    29 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    2 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    149 digestive system, larvae L3 wandering    29 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    2 fat body, larvae L3 wandering    0 fat body, white prepupae    279 fat body, pupae P8    117 carcass, larvae L3 wandering    796 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    7 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (1313) central nervous system, larvae L3  (199) central nervous system, pupae P8  29 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    2 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae  (149) digestive system, larvae L3 wandering  29 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    2 fat body, larvae L3 wandering    0 fat body, white prepupae  (279) fat body, pupae P8  (117) carcass, larvae L3 wandering  (796) carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    7 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (1313) central nervous system, larvae L3  (199) central nervous system, pupae P8    29 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    2 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae  (149) digestive system, larvae L3 wandering    29 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    2 fat body, larvae L3 wandering    0 fat body, white prepupae  (279) fat body, pupae P8  117 carcass, larvae L3 wandering  (796) carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    7 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    1313 central nervous system, larvae L3    199 central nervous system, pupae P8    29 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    2 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    149 digestive system, larvae L3 wandering    29 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    2 fat body, larvae L3 wandering    0 fat body, white prepupae    279 fat body, pupae P8    117 carcass, larvae L3 wandering    796 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    7 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of extremely high expression to a trough of extremely low expression. Peak expression observed during early pupal stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    3 embryo 02-04hr    23 embryo 04-06hr    49 embryo 06-08hr    17 embryo 08-10hr    16 embryo 10-12hr    117 embryo 12-14hr    480 embryo 14-16hr    529 embryo 16-18hr    51 embryo 18-20hr    6 embryo 20-22hr    1 embryo 22-24hr    3 larva L1    2 larva L2    71 larva L3 12hr old    18 larva L3 puffstage 1-2    25 larva L3 puffstage 3-6    197 larva L3 puffstage 7-9    582 white prepupae new    862 white prepupae 12hr    38 white prepupae 24hr    1910 pupae 2d postWPP    392 pupae 3d postWPP    28 pupae 4d postWPP    2 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    3 embryo 02-04hr    23 embryo 04-06hr  (49) embryo 06-08hr    17 embryo 08-10hr    16 embryo 10-12hr  (117) embryo 12-14hr  (480) embryo 14-16hr  (529) embryo 16-18hr  (51) embryo 18-20hr    6 embryo 20-22hr    1 embryo 22-24hr    3 larva L1    2 larva L2  (71) larva L3 12hr old    18 larva L3 puffstage 1-2    25 larva L3 puffstage 3-6  (197) larva L3 puffstage 7-9  (582) white prepupae new  (862) white prepupae 12hr  (38) white prepupae 24hr  (1910) pupae 2d postWPP  (392) pupae 3d postWPP  (28) pupae 4d postWPP    2 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    3 embryo 02-04hr    23 embryo 04-06hr    49 embryo 06-08hr    17 embryo 08-10hr    16 embryo 10-12hr  (117) embryo 12-14hr  (480) embryo 14-16hr  (529) embryo 16-18hr    51 embryo 18-20hr    6 embryo 20-22hr    1 embryo 22-24hr    3 larva L1    2 larva L2    71 larva L3 12hr old    18 larva L3 puffstage 1-2    25 larva L3 puffstage 3-6  (197) larva L3 puffstage 7-9  (582) white prepupae new  (862) white prepupae 12hr    38 white prepupae 24hr  (1910) pupae 2d postWPP  (392) pupae 3d postWPP    28 pupae 4d postWPP    2 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    3 embryo 02-04hr    23 embryo 04-06hr    49 embryo 06-08hr    17 embryo 08-10hr    16 embryo 10-12hr    117 embryo 12-14hr    480 embryo 14-16hr    529 embryo 16-18hr    51 embryo 18-20hr    6 embryo 20-22hr    1 embryo 22-24hr    3 larva L1    2 larva L2    71 larva L3 12hr old    18 larva L3 puffstage 1-2    25 larva L3 puffstage 3-6    197 larva L3 puffstage 7-9    582 white prepupae new    862 white prepupae 12hr    38 white prepupae 24hr    1910 pupae 2d postWPP    392 pupae 3d postWPP    28 pupae 4d postWPP    2 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    3 embryo 02-04hr    23 embryo 04-06hr    49 embryo 06-08hr    17 embryo 08-10hr    16 embryo 10-12hr    117 embryo 12-14hr    480 embryo 14-16hr    529 embryo 16-18hr    51 embryo 18-20hr    6 embryo 20-22hr    1 embryo 22-24hr    3 larva L1    2 larva L2    71 larva L3 12hr old    18 larva L3 puffstage 1-2    25 larva L3 puffstage 3-6    197 larva L3 puffstage 7-9    582 white prepupae new    862 white prepupae 12hr    38 white prepupae 24hr    1910 pupae 2d postWPP    392 pupae 3d postWPP    28 pupae 4d postWPP    2 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    3 embryo 02-04hr    23 embryo 04-06hr  (49) embryo 06-08hr    17 embryo 08-10hr    16 embryo 10-12hr  (117) embryo 12-14hr  (480) embryo 14-16hr  (529) embryo 16-18hr  (51) embryo 18-20hr    6 embryo 20-22hr    1 embryo 22-24hr    3 larva L1    2 larva L2  (71) larva L3 12hr old    18 larva L3 puffstage 1-2    25 larva L3 puffstage 3-6  (197) larva L3 puffstage 7-9  (582) white prepupae new  (862) white prepupae 12hr  (38) white prepupae 24hr  (1910) pupae 2d postWPP  (392) pupae 3d postWPP  28 pupae 4d postWPP    2 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    3 embryo 02-04hr    23 embryo 04-06hr    49 embryo 06-08hr    17 embryo 08-10hr    16 embryo 10-12hr  117 embryo 12-14hr  (480) embryo 14-16hr  (529) embryo 16-18hr    51 embryo 18-20hr    6 embryo 20-22hr    1 embryo 22-24hr    3 larva L1    2 larva L2    71 larva L3 12hr old    18 larva L3 puffstage 1-2    25 larva L3 puffstage 3-6  (197) larva L3 puffstage 7-9  (582) white prepupae new  (862) white prepupae 12hr    38 white prepupae 24hr  (1910) pupae 2d postWPP  (392) pupae 3d postWPP    28 pupae 4d postWPP    2 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    3 embryo 02-04hr    23 embryo 04-06hr    49 embryo 06-08hr    17 embryo 08-10hr    16 embryo 10-12hr    117 embryo 12-14hr    480 embryo 14-16hr    529 embryo 16-18hr    51 embryo 18-20hr    6 embryo 20-22hr    1 embryo 22-24hr    3 larva L1    2 larva L2    71 larva L3 12hr old    18 larva L3 puffstage 1-2    25 larva L3 puffstage 3-6    197 larva L3 puffstage 7-9    582 white prepupae new    862 white prepupae 12hr    38 white prepupae 24hr    1910 pupae 2d postWPP    392 pupae 3d postWPP    28 pupae 4d postWPP    2 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    42 cold shock, 4-day adult    44 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    10 Cadmium 50 mM 12 hrs, larvae L3    179 Cadmium 50 mM 48 hrs, 4-day adult    2 Cadmium 100 mM 48 hrs, 4-day adult    1 Copper 0.5 mM 12 hrs, larvae L3    13 Copper 15 mM 48 hrs, 4-day adult    3 Zinc 5 mM 12 hrs, larvae L3    23 Zinc 4.5 mM 48 hrs, 4-day adult    9 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    7 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    5 Caffeine 25 mg/ml 48 hrs, 4-day adult    19 Paraquat 5 mM 48 hrs, 4-day adult    1 Paraquat 10 mM 48 hrs, 4-day adult    4 Rotenone 2 μg 12 hrs, larvae L3    16 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  (42) cold shock, 4-day adult  (44) heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    10 Cadmium 50 mM 12 hrs, larvae L3  (179) Cadmium 50 mM 48 hrs, 4-day adult    2 Cadmium 100 mM 48 hrs, 4-day adult    1 Copper 0.5 mM 12 hrs, larvae L3    13 Copper 15 mM 48 hrs, 4-day adult    3 Zinc 5 mM 12 hrs, larvae L3    23 Zinc 4.5 mM 48 hrs, 4-day adult    9 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    7 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    5 Caffeine 25 mg/ml 48 hrs, 4-day adult    19 Paraquat 5 mM 48 hrs, 4-day adult    1 Paraquat 10 mM 48 hrs, 4-day adult    4 Rotenone 2 μg 12 hrs, larvae L3    16 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    42 cold shock, 4-day adult    44 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    10 Cadmium 50 mM 12 hrs, larvae L3  (179) Cadmium 50 mM 48 hrs, 4-day adult    2 Cadmium 100 mM 48 hrs, 4-day adult    1 Copper 0.5 mM 12 hrs, larvae L3    13 Copper 15 mM 48 hrs, 4-day adult    3 Zinc 5 mM 12 hrs, larvae L3    23 Zinc 4.5 mM 48 hrs, 4-day adult    9 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    7 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    5 Caffeine 25 mg/ml 48 hrs, 4-day adult    19 Paraquat 5 mM 48 hrs, 4-day adult    1 Paraquat 10 mM 48 hrs, 4-day adult    4 Rotenone 2 μg 12 hrs, larvae L3    16 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    42 cold shock, 4-day adult    44 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    10 Cadmium 50 mM 12 hrs, larvae L3    179 Cadmium 50 mM 48 hrs, 4-day adult    2 Cadmium 100 mM 48 hrs, 4-day adult    1 Copper 0.5 mM 12 hrs, larvae L3    13 Copper 15 mM 48 hrs, 4-day adult    3 Zinc 5 mM 12 hrs, larvae L3    23 Zinc 4.5 mM 48 hrs, 4-day adult    9 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    7 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    5 Caffeine 25 mg/ml 48 hrs, 4-day adult    19 Paraquat 5 mM 48 hrs, 4-day adult    1 Paraquat 10 mM 48 hrs, 4-day adult    4 Rotenone 2 μg 12 hrs, larvae L3    16 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    42 cold shock, 4-day adult    44 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    10 Cadmium 50 mM 12 hrs, larvae L3    179 Cadmium 50 mM 48 hrs, 4-day adult    2 Cadmium 100 mM 48 hrs, 4-day adult    1 Copper 0.5 mM 12 hrs, larvae L3    13 Copper 15 mM 48 hrs, 4-day adult    3 Zinc 5 mM 12 hrs, larvae L3    23 Zinc 4.5 mM 48 hrs, 4-day adult    9 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    7 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    5 Caffeine 25 mg/ml 48 hrs, 4-day adult    19 Paraquat 5 mM 48 hrs, 4-day adult    1 Paraquat 10 mM 48 hrs, 4-day adult    4 Rotenone 2 μg 12 hrs, larvae L3    16 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  (42) cold shock, 4-day adult  (44) heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    10 Cadmium 50 mM 12 hrs, larvae L3  (179) Cadmium 50 mM 48 hrs, 4-day adult    2 Cadmium 100 mM 48 hrs, 4-day adult    1 Copper 0.5 mM 12 hrs, larvae L3    13 Copper 15 mM 48 hrs, 4-day adult    3 Zinc 5 mM 12 hrs, larvae L3    23 Zinc 4.5 mM 48 hrs, 4-day adult    9 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    7 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    5 Caffeine 25 mg/ml 48 hrs, 4-day adult    19 Paraquat 5 mM 48 hrs, 4-day adult    1 Paraquat 10 mM 48 hrs, 4-day adult    4 Rotenone 2 μg 12 hrs, larvae L3    16 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    42 cold shock, 4-day adult    44 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    10 Cadmium 50 mM 12 hrs, larvae L3  (179) Cadmium 50 mM 48 hrs, 4-day adult    2 Cadmium 100 mM 48 hrs, 4-day adult    1 Copper 0.5 mM 12 hrs, larvae L3    13 Copper 15 mM 48 hrs, 4-day adult    3 Zinc 5 mM 12 hrs, larvae L3    23 Zinc 4.5 mM 48 hrs, 4-day adult    9 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    7 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    5 Caffeine 25 mg/ml 48 hrs, 4-day adult    19 Paraquat 5 mM 48 hrs, 4-day adult    1 Paraquat 10 mM 48 hrs, 4-day adult    4 Rotenone 2 μg 12 hrs, larvae L3    16 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    42 cold shock, 4-day adult    44 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    10 Cadmium 50 mM 12 hrs, larvae L3    179 Cadmium 50 mM 48 hrs, 4-day adult    2 Cadmium 100 mM 48 hrs, 4-day adult    1 Copper 0.5 mM 12 hrs, larvae L3    13 Copper 15 mM 48 hrs, 4-day adult    3 Zinc 5 mM 12 hrs, larvae L3    23 Zinc 4.5 mM 48 hrs, 4-day adult    9 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    7 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    5 Caffeine 25 mg/ml 48 hrs, 4-day adult    19 Paraquat 5 mM 48 hrs, 4-day adult    1 Paraquat 10 mM 48 hrs, 4-day adult    4 Rotenone 2 μg 12 hrs, larvae L3    16 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_25
External Data & Images
Linkouts	BDGP expression data • LD19609 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0001254 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG1934-RA FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for ImpE2 Stages(s) 4-6 Stages(s) 7-8 Stages(s) 9-10 Stages(s) 11-12 Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable, with Scer\GAL4pnr-MD237 ImpE2GD5839 Phenotype manifest in Allele
Classical Alleles ( 0 )
For All Classical Alleles Show
Allele of ImpE2 Class Mutagen Stocks Known lesion
Alleles Carried on Transgenic Constructs ( 4 )
For All Alleles Carried on Transgenic Constructs Show
Allele of ImpE2 Class Mutagen Stocks Known lesion ImpE2GD5839 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes ImpE2GL00120 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes ImpE2KK105038 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes ImpE2NIG.1934R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3L)Exel6095 (Ryder, 2004.4.26) Df(3L)HR119 (Paine-Saunders et al., 1990)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD5839} NA P{KK105038} NA P{NIG.1934R} NA P{TRiP.GL00120} NA
Insertions
	Type of insertions Name Expression data
Gene Ontology: Function, Process & Cellular Component ( 4 unique terms )
Terms Based on Experimental Evidence ( 2 terms )
Molecular Function ( 0 terms)
Biological Process ( 0 terms)
Cellular Component
CV term Evidence References
cytoplasm inferred from direct assay (Paine-Saunders et al., 1990) extracellular region inferred from direct assay (Paine-Saunders et al., 1990)
Terms Based on Predictions or Assertions ( 3 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
imaginal disc eversion traceable author statement (Andres et al., 1993)
Cellular Component
CV term Evidence References
extracellular region non-traceable author statement (Paine-Saunders, 1991.1.30) extrinsic to membrane traceable author statement (Andres et al., 1993)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
ImpE2 - Arpc2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) ImpE2 - CG4325 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) ImpE2 - Cht2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) ImpE2 - Hsc70-1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) ImpE2 - abs anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions ImpE2 allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 63925 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0001254 DroID - A comprehensive database of gene and protein interactions. • FBgn0001254 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 38432
Orthologs
OrthoDB Orthologs (4) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6TQMWZ Dipteran inclusive ortholog search No orthologs identified Insect inclusive ortholog search No orthologs identified Arthropod inclusive ortholog search No orthologs identified Metazoa inclusive ortholog search No orthologs identified
Orthologs in Drosophila Species (EOG6TQMWZ)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\ImpE2     Drosophila simulans   Dsim\GD13325 Y   Drosophila sechellia   Dsec\GM14048 Y   Drosophila virilis   Dvir\GJ12959 Y
Orthologs in non-Drosophila Dipterans (None identified)
No non-Drosophilid orthologies identified
Orthologs in non-Dipteran Insects (None identified)
No non-Dipteran orthologies identified
Orthologs in non-Insect Arthropods (None identified)
No non-Insect Arthropod orthologies identified
Orthologs in non-Arthropod Metazoa (None identified)
No non-Arthropod Metazoa orthologies identified
Human Orthologs (0)
Gene OMIM HGNC
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD13325 Drosophila sechellia Dsec\GM14048 Drosophila erecta Dere\GG14254 Drosophila yakuba Dyak\GE20682 Drosophila ananassae Dana\GF10264 Drosophila pseudoobscura pseudoobscura Dpse\GA15137 Drosophila persimilis Dper\GL13255 Drosophila willistoni Dwil\GK20372 Drosophila virilis Dvir\GJ12959 Drosophila mojavensis Dmoj\GI12817 Drosophila grimshawi Dgri\GH15050
Stocks & Reagents
Stocks Listed in FlyBase ( 3 )
Bloomington	35235 y1 sc* v1; P{TRiP.GL00120}attP2
VDRC	v100148 P{KK105038}VIE-260B v16842 w1118; P{GD5839}v16842/CyO
Genomic Clones ( 2 )
	BACR09B04 BACR22N09 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 62 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD19609
Other clones	DPiM_LD19609
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	LP02336 LP04186 LP04921 LP04943 LP05279 LP06952 LP07016 LP07355 LP07985 LP08783 LP10170 LP10416 LP15427 LP15629 LP15871 LP15993 LP16918 LP18145 LP19163 LP19519 LP19862 LP19989 LP20137 LP20776 LP20856 LP22066 LP24011 RE09796 RE44285 RE50912 RE52312
Other clones	14496 16736 59611 78495 78796 80405 156349 CK01.13A.G4 CK01.60B.E11 CK01.60C.E9 CK01.86B.B2 CK01.87D.H3 CK01.132D.G5 CK01.133B.A8 EC28277 EC40060 EK048718 EK063519 EK068137 EK069330 EK101227 EK101936 EK116550 EK128049 EK207940 EK213641 EK259154 EK259303 EP21303
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0001254 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 38432
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: ImpE2 CG1934 (Kodira, 1999.11)
Source for database merge of
Additional comments	"ImpE2" may correspond to the "l(3)63Dc" locus. (Munroe et al., 1998.5.12)
Other Comments
	Expression throughout the onset of metamorphosis is not affected by Eip74EF mutations. (Fletcher and Thummel, 1995) Ecdysteroid-regulated gene. (Andres et al., 1993) ImpE2 has been sequenced and its expression pattern analysed. (Paine-Saunders et al., 1990) Gene encoding a transcript associated with membrane-bound polysomes in imaginal discs and expressed only in response to 20-hydroxyecdysone (20 HOE). Expression studied both in vitro and in vivo. One of the "early" genes involved in elongation of appendage-forming regions of the imaginal discs by means of epithelial-cell rearrangements (Natzle, Fristrom and Fristrom, 1988).
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_057232 RefSeq (Proteins) • NP_476580 DDBJ / EMBL / GenBank • AA540201 AAA63632 AAF47780 AAL28818 AC004658 AW942536 AY061270 M55099 Entrez Gene - A searchable database of RefSeq genes. • 38432 UniProtKB/Swiss-Prot • P29681
Other Crossreferences
	BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • LD19609
Linkouts
	BioGRID - A database of protein and genetic interactions • 63925 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0001254 DroID - A comprehensive database of gene and protein interactions. • FBgn0001254 DRSC - Results from RNAi screens. • FBgn0001254 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0001254 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG1934-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0001254 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 38432 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 38432 modMine - Data generated by the modENCODE project. • FBgn0001254
Synonyms & Secondary IDs ( 5 )
Reported As
Symbol Synonym	CG1934 (Carrera et al., 2008, Keleman et al., 2009.8.5, Haussmann et al., 2008, Ni et al., 2011.7.19) IMP-E2 (Paine-Saunders, 1991.1.30, Fristrom and Fristrom, 1993, Andres and Thummel, 1992, Paine-Saunders et al., 1990, Thummel, 1990, Beckstead et al., 2005) Imp-E2 (Bayer et al., 1996, Fletcher and Thummel, 1995, Andres et al., 1993, ) ImpE2 (Bochdanovits and de Jong, 2004, Gim et al., 2001, Zeitouni et al., 2007, Kugler et al., 2011, Carrera et al., 2008, Kwon et al., 2009, Weake et al., 2009, Beltran et al., 2007, Tomancak et al., 2007, van der Knaap et al., 2010, Japanese National Institute of Genetics, 2012.5.21)
Name Synonym	Ecdysone-inducible gene E2 (Gim et al., 2001)
Secondary FlyBase IDs
References ( 46 )
Generate a list of	All references relating to this gene ( 46 )
List References by type	Research paper  ( 23 ) Supplementary material  ( 3 ) Review  ( 4 ) Personal communication to FlyBase  ( 8 ) FlyBase analysis  ( 2 ) DNA/RNA sequence record  ( 1 ) Stock list  ( 1 ) Curated genome annotation  ( 2 ) Website  ( 1 ) Book  ( 1 )
Recent research papers ( 1 )
	Kugler et al., 2011, Genetics 188(1): 127--139 The Putzig-NURF Nucleosome Remodeling Complex Is Required for Ecdysone Receptor Signaling and Innate Immunity in Drosophila melanogaster. [FBrf0213648] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...