FlyBase Gene Report: Dmel\ImpE3

General Information

Symbol

Dmel\ImpE3

Species

D. melanogaster

Name

Ecdysone-inducible gene E3

Annotation Symbol

CG2723

Feature Type

protein_coding_gene

FlyBase ID

FBgn0001255

Gene Model Status

Current

Stock Availability

3 publicly available

Gene Summary

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Red Book
Interactive Fly

Also Known As

Imp-E3

Key Links

Genomic Location

Cytogenetic map

84E8-84E9

Sequence location

3R:8,028,453..8,029,546 [-]

Recombination map

3-48

RefSeq locus

NT_033777 REGION:8028453..8029546

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (3 terms)

Molecular Function (0 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in imaginal disc eversion

traceable author statement

(Andres et al., 1993)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

located_in extrinsic component of membrane

traceable author statement

(Andres et al., 1993)

located_in plasma membrane

inferred from sequence or structural similarity

(Natzle et al., 1988)

Gene Group (FlyBase)

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Phenotypic Description (Red Book; Lindsley and Zimm 1992)

Imp-E: Inducible membrane-bound polysomes-Early

Name given to three genes encoding transcripts associated with membrane-bound polysomes in imaginal discs and expressed only in response to 20-hydroxyecdysone (20 HOE). Expression studied both in vitro and in vivo. Imp-E denotes three "early" genes involved in elongation of appendage-forming regions of the imaginal discs by means of epithelial-cell rearrangements (Natzle et al., 1988).

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\ImpE3 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9VHW8)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.43

Gene model reviewed during 5.47

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

ImpE3-RB

FBtr0310412

NM_080169

1094

325

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

ImpE3-PB

FBpp0302560

36.0

325

5.84

Q9VHW8

NP_524908

AAF54181

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Q9VHW8

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\ImpE3 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

ImpE3_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.44

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\ImpE3 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\ImpE3

View exonic expression by tissue for Dmel\ImpE3

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

48239/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0001255

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0001255

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0001255

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG2723

Flygut - An atlas of the Drosophila adult midgut

FBgn0001255

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 1 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 2 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of ImpE3

Transgenic constructs containing regulatory region of ImpE3

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

Df(3R)Exel6263

(Ryder, 2004.4.26)

Inferred from location ( 13 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

ImpE3^GD5570

viable, with Scer\GAL4^Mef2.PR

ImpE3^GD5570

viable, with Scer\GAL4^pnr-MD237

ImpE3^GD5570

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (0)

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (0)

Mus musculus (laboratory mouse) (0)

Xenopus tropicalis (Western clawed frog) (0)

Danio rerio (Zebrafish) (0)

Caenorhabditis elegans (Nematode, roundworm) (0)

Anopheles gambiae (African malaria mosquito) (1)

Agam\AgaP_AGAP003032

9 of 12

Yes

Arabidopsis thaliana (thale-cress) (0)

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:ImpE3. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (0)

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Other Interaction Browsers

Summary of Genetic Interactions

esyN Network Diagram

Other Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

71240

DroID - A comprehensive database of gene and protein interactions.

FBgn0001255

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-48

Cytogenetic map

84E8-84E9

Sequence location

3R:8,028,453..8,029,546 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

84E8-84E9

Limits computationally determined from genome sequence between P{EP}EP3060^EP3060 and P{PZ}grn⁰⁵⁹³⁰

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

84E-84E

(determined by in situ hybridisation)

(Moore et al., 1990, Natzle et al., 1986)

Experimentally Determined Recombination Data

Location

3-48

(FlyBase, 2016)

3-44.7

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (3)

Aberrations including deletions of this gene Aberrations including duplications of this gene

NIG-Fly

2723R-2

P{NIG.2723R}2

VDRC

v16403

w¹¹¹⁸; P{GD5570}v16403

v16402

w¹¹¹⁸; P{GD5570}v16402/TM3

Genomic Clones (27)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (7)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

LD11958

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0001255

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0001255

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Expression throughout the onset of metamorphosis is not affected by Eip74EF mutations.

(Fletcher and Thummel, 1995)

Ecdysteroid-regulated gene.

(Andres et al., 1993)

ImpE3 has been cloned and sequenced, and its RNA expression pattern has been analysed.

(Moore et al., 1990)

Gene encoding a transcript associated with membrane-bound polysomes in imaginal discs and expressed only in response to 20-hydroxyecdysone (20 HOE). Expression studied both in vitro and in vivo. One of the "early" genes involved in elongation of appendage-forming regions of the imaginal discs by means of epithelial-cell rearrangements (Natzle, Fristrom and Fristrom, 1988).

Relationship to Other Genes

Source for database merge of

Source for merge of: ImpE3 CG2723

(Misra, 2000.7.28)

Source for merge of: ImpE3 CG11046

(FlyBase, 1996-)

Additional comments

Release 1 annotation CG11046 corresponds to part of the release 3.2 annotation for ImpE3 (CG2723).

(FlyBase, 1996-)

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

CG11046

(Badenhorst et al., 2005)

CG2723

(Badenhorst et al., 2005)

IMP-E3

(Andres and Thummel, 1992, Moore et al., 1990, Thummel, 1990)

Imp-E3

(Bayer et al., 1996, Fletcher and Thummel, 1995, Andres et al., 1993)

ImpE3

(Ou et al., 2016, Xie et al., 2015, Japanese National Institute of Genetics, 2012.5.21, Stern et al., 2012, Gilchrist et al., 2008, Pearse et al., 2008, Zeitouni et al., 2007)

Name Synonyms

Ecdysone-inducible gene E3

(Rice et al., 2020)

Secondary FlyBase IDs

FBgn0037542
FBgn0040525

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 28 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

48239

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9VHW8

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Q9VHW8

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9VHW8

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

48239/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0001255

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0001255

FlyMine - An integrated database for Drosophila genomics

FBgn0001255

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG2723

MARRVEL_MODEL - MARRVEL (model organism gene)

48239

Linkouts

BioGRID - A database of protein and genetic interactions.

71240

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0001255

DroID - A comprehensive database of gene and protein interactions.

FBgn0001255

DRSC - Results frm RNAi screens

FBgn0001255

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

ImpE3_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0001255

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0001255

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG2723

Flygut - An atlas of the Drosophila adult midgut

FBgn0001255

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC015109

References (44)

The following external sites may use different assemblies or annotations than FlyBase.

-0.44

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