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FB2025_05
,
released December 11, 2025
Imp-L2, Imaginal morphogenesis protein-Late 2, ImpL-2, Imaginal morphogenesis protein late 2
a secreted protein that binds Drosophila insulin-like peptide 2 and inhibits growth non-autonomously
Please see the JBrowse view of Dmel\ImpL2 for information on other features
To submit a correction to a gene model please use the Contact FlyBase form
AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.
Low-frequency RNA-Seq exon junction(s) not annotated.
Annotated transcripts do not represent all supported alternative splices within 5' UTR.
Gene model reviewed during 5.46
Gene model reviewed during 6.32
2.4 (northern blot)
263 (aa); 32 (kD observed); 27 (kD predicted)
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\ImpL2 using the Feature Mapper tool.
The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).
Comment: anlage in statu nascendi
Comment: anlage in statu nascendi
ImpL2 is expressed in anterior cells of the tracheal pit and in cells immediately dorsal to the pit invagination. It is expressed only in a subset of cells in the tracheal primordium.
ImpL2 transcripts are not detected until 4hr of embyronic development on northern blots. By in situ hybridization, they are detected in blastoderm embryos in a pair-rule-like pattern of seven stripes. The stripes extend through the neurogenic and dorsal epidermal regions but not extend to the presumptive mesoderm or amnioserosa. At stage 8, 14 broad bands are seen in the neurogenic ectoderm. At stage 9, strong expression is seen surrounding the tracheal pits. During stages 15-16, ImpL2 transcripts are detected in the pharynx, frontal sac, the esophagus, surrounding the posterior spiracles, and the lateral bipolar dendrite neurons. By stage 17, they are limited to the lateral bipolar dendrite neurons and other structures that show accumulation of protein.
ImpL2 protein is weakly detected in embryonic extracts from 2-4hrs and a stronger signal is seen in 4-6hr and 6-8hr embryos. No localized ImpL2 protein was detected until stage 16 at which time a segmentally repeated pattern of staining in the lateral region is observed. The stained cells in the abdominal segments were identified as the lateral bipolar dendrite neurons. In the thoracic segments, similarly positioned neuronal cell bodies and their axons were stained but their identity is uncertain. They are probably multiple dendritic neurons. Staining is also observed in two neurons adjacent to the pharynx, in two cell clusters on the dorsolateral surface of the optic lobes, and in a localized area of the cephalic region.
JBrowse - Visual display of RNA-Seq signals
View Dmel\ImpL2 in JBrowse
3-10
3-9.2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
polyclonal
In a sample of 79 genes with multiple introns, 33 showed significant heterogeneity in G+C content among introns of the same gene and significant positive correspondence between the intron and the third codon position G+C content within genes. These results are consistent with selection adding against preferred codons at the start of genes.
Temporal profile of gene expression is altered in Eip74EF mutant background.
Ecdysteroid-regulated gene.
Encodes a transcript associated with membrane-bound polysomes in imaginal discs and expressed only in response to 20-hydroxyecdysone (20 HOE). Expression studied both in vitro and in vivo. A 'late' gene involved in the eversion to the exterior of the elongated regions of discs by means of local changes in cell shape (Osterbur, Fristrom, Natzle, Tojo and Fristrom, 1988).
