Gene Dmel\ImpL3

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General Information

Symbol

Dmel\ImpL3

Species

D. melanogaster

Name

Ecdysone-inducible gene L3

Annotation symbol

CG10160

Feature type

protein_coding_gene

FlyBase ID

FBgn0001258

Gene Model Status

Current

Stock availability

2 publicly available

Also Known As

IMP-L3, LDH

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

65A11-65A11

Sequence location

3L:6,252,265..6,255,794 [-]

Genomic Maps

Select View:

Summary Information

Automatically generated summary

See sections below for more information

The gene Ecdysone-inducible gene L3 is referred to in FlyBase by the symbol Dmel\ImpL3 (CG10160, FBgn0001258). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: L-lactate dehydrogenase activity. It is reported to be involved in the biological process: glycolysis. 4 alleles are reported. No phenotypic data is available. It has 4 annotated transcripts and 4 annotated polypeptides. Protein features are: L-lactate dehydrogenase; L-lactate dehydrogenase, active site; L-lactate/malate dehydrogenase; Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal; Lactate/malate dehydrogenase, C-terminal; Lactate/malate dehydrogenase, N-terminal; NAD(P)-binding domain. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of low expression. Peak expression observed within 12-24 hour embryonic stages, at stages throughout the larval period. Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: larval central nervous system, adult midgut, larval hindgut, virgin spermatheca, larval carcass. Expression at moderate levels in the following post-embryonic organs or tissues: adult crop, larval midgut, adult hindgut, adult heart, adult fat body, larval trachea, inseminated spermatheca, adult testis, adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1635227_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of ImpL3. Gene sequence location is 3L:6252265..6255794.

User Contributed Data

Beta version, feedback welcome

Phenotypic Description from the Red Book (Lindsley & Zimm 1992)

Gene/Allele symbols may differ from current usage

Imp-L: Imp-Late

Imp-L encoding transcripts associated with membrane-bound polysomes in imaginal discs and expressed only in response to 20-hydroxyecdysone (20 HOE). Expression studied both in vitro and in vivo. Imp-L denotes three "late" genes involved in the eversion to the exterior of the elongated regions of discs by means of local changes in cell shape (Osterbur et al., 1988).

Recent Updates

Description

What does this section display?

This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).

What does this section not display?

This section does not currently display links that were removed or gene model changes.

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FB2013_03

FB2013_02

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Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

65A11-65A11

Limits computationally determined from genome sequence between P{PZ}l(3)02094⁰²⁰⁹⁴ and P{lacW}l(3)L4060^L4060

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

65B-65B

(determined by in situ hybridisation)

(Abu-Shumays and Fristrom, 1997)

65B-65B

(determined by in situ hybridisation)

(Natzle et al., 1986)

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Gene Model & Products

Please see the GBrowse view of Dmel\ImpL3 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Comments on Gene Model

Gene model reviewed during 5.45

Gene model reviewed during 5.51

Low-frequency RNA-seq exon junction(s) not annotated.

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

ImpL3-RA

FBtr0077008

NM_057233

1391

332

ImpL3-RB

FBtr0332065

NM_001274545

1718

332

ImpL3-RC

FBtr0332066

NM_001274546

1324

332

ImpL3-RD

FBtr0332067

NM_001274547

1350

332

Additional Transcript Data & Comments

Reported size (kB)

1.5 (northern blot)

(Abu-Shumays and Fristrom, 1997)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

ImpL3-PA

FBpp0076716

35.5

332

7.10

NP_476581

AAF50666

ImpL3-PB

FBpp0304375

35.5

332

7.10

NP_001261474

AGB94169

ImpL3-PC

FBpp0304376

35.5

332

7.10

NP_001261475

AGB94170

ImpL3-PD

FBpp0304377

35.5

332

7.10

NP_001261476

AGB94171

Additional Polypeptide Data & Comments

Reported size (kDa)

332 (aa)

(Abu-Shumays and Fristrom, 1997)

Comments

External Data

Linkouts

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal (IPR015955)

L-lactate dehydrogenase, active site (IPR018177)

Lactate/malate dehydrogenase, N-terminal (IPR001236)

L-lactate/malate dehydrogenase (IPR001557)

L-lactate dehydrogenase (IPR011304)

NAD(P)-binding domain (IPR016040)

Lactate/malate dehydrogenase, C-terminal (IPR022383)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Q95028

UniProtKB/TrEMBL

Mapped Features

Mapped Features have been reorganized, please see this article for details.

Additional mapped features and mutations can be found on GBrowse or related reports.

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

Crossreferences

Expression Data

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 4 -- 6

anterior endoderm anlage

(Fisher et al., 2012)

Comment:anlage in statu nascendi

foregut anlage in statu nascendi

(Fisher et al., 2012)

embryonic stage 11 -- 12

head mesoderm

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic/larval muscle system

(Fisher et al., 2012)

embryonic/larval posterior spiracle

(Fisher et al., 2012)

embryonic/larval somatic muscle

(Fisher et al., 2012)

prothoracic gland

(Fisher et al., 2012)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage & larval stage & adult stage

(Abu-Shumays and Fristrom, 1997)

embryonic stage 15 -- 17

pharyngeal muscle

(Abu-Shumays and Fristrom, 1997)

embryonic/larval posterior spiracle

(Abu-Shumays and Fristrom, 1997)

embryonic/larval proventriculus

(Abu-Shumays and Fristrom, 1997)

larval stage

imaginal disc

(Abu-Shumays and Fristrom, 1997)

Additional Descriptive Data

ImpL3 transcripts are abundant during late embryogenesis and in larval stages. Transcripts are present at low levels in pupae and increase again in adults. ImpL3 transcripts are detected in cultured imaginal discs and levels increase with exposure to 20-hydroxyecdysone. In situ hybridization shows that ImpL3 transcripts are abundantly expressed in somatic muscles from stage 13 on and are detected as early as stage 11. Staining is also observed in the posterior spiracles, pharyngeal muscles, and proventriculus by stage 15.

(Abu-Shumays and Fristrom, 1997)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

enzyme assay or biochemical detection

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

embryonic/larval somatic muscle

(Abu-Shumays and Fristrom, 1997)

embryonic stage 15 -- 17

embryonic/larval posterior spiracle

(Abu-Shumays and Fristrom, 1997)

embryonic/larval proventriculus

(Abu-Shumays and Fristrom, 1997)

pharyngeal muscle

(Abu-Shumays and Fristrom, 1997)

prepupal stage

tibia | presumptive

(Abu-Shumays and Fristrom, 1997)

tarsal segment

(Abu-Shumays and Fristrom, 1997)

ventral thoracic disc

(Abu-Shumays and Fristrom, 1997)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

Additional Descriptive Data

Levels of ImpL3 protein were measured by assaying for LDH activity. LDH activity is detected in cultured imaginal discs and is significantly higher in discs cultured with 20-hydroxyecdysone. LDH activity was found to be low in third instar larval leg discs but high in prepupal leg discs in the tarsal segments and the presumptive tibia. LDH activity localizes to somatic mesoderm and later to somatic muscle cells in embryos. Activity is also detected in the posterior spiracles, pharyngeal muscles, and proventriculus by stage 15.

(Abu-Shumays and Fristrom, 1997)

Marker for

Subcellular Localization (GO Cellular Component)

CV term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

Reference

See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.

FlyAtlas Anatomy Microarray

FlyAtlas Anatomical Expression Data

(FlyAtlas-RNA.adult)

(FlyAtlas-RNA.larva)

	Styles
	Linear
	Logarithmic
	Heatmap
	Back-to-back
	Scales
	gene max expression
	Moderate expression bin max
	High level expression bin max
	Very high expression bin max

Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: larval central nervous system, adult midgut, larval hindgut, virgin spermatheca, larval carcass. Expression at moderate levels in the following post-embryonic organs or tissues: adult crop, larval midgut, adult hindgut, adult heart, adult fat body, larval trachea, inseminated spermatheca, adult testis, adult carcass.
[download data (TSV)]

Guide to FlyAtlas expression level colors
	No expression (0 - 9.999)
	Low expression (10 - 99.999)
	Moderate expression (100 - 499.999)
	High level expression (500 - 999.999)
	Very high expression (>999.999)

Linear, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		1180.9
Larval Midgut		115.9
Larval Hindgut		594
Larval Malpighian Tubules		73.3
Larval Fat Body		3.3
Larval Salivary Gland		96.4
Larval Trachea		291.075
Larval Carcass		4283.075
Adult Head		60.7
Adult Eye		13.7
Adult Brain		25.9
Adult Thoracic-Abdominal Ganglion		14.3
Adult Crop		375
Adult Midgut		872
Adult Hindgut		281.8
Adult Malpighian Tubules		33.6
Adult Fat Body		435.2
Adult Salivary Gland		138.4
Adult Heart		375.325
Adult VirginFemale Spermatheca		600.5
Adult InseminatedFemale Spermatheca		370.7
Adult Ovary		1.9
Adult Testis		118.4
Adult Male Accessory Gland		28.8
Adult Carcass		183.2
Expression Level Scale		None Low Moderate High Very high

Linear, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		(1180.9)
Larval Midgut		115.9
Larval Hindgut		(594)
Larval Malpighian Tubules		73.3
Larval Fat Body		3.3
Larval Salivary Gland		96.4
Larval Trachea		291.075
Larval Carcass		(4283.075)
Adult Head		60.7
Adult Eye		13.7
Adult Brain		25.9
Adult Thoracic-Abdominal Ganglion		14.3
Adult Crop		375
Adult Midgut		(872)
Adult Hindgut		281.8
Adult Malpighian Tubules		33.6
Adult Fat Body		435.2
Adult Salivary Gland		138.4
Adult Heart		375.325
Adult VirginFemale Spermatheca		(600.5)
Adult InseminatedFemale Spermatheca		370.7
Adult Ovary		1.9
Adult Testis		118.4
Adult Male Accessory Gland		28.8
Adult Carcass		183.2
Expression Level Scale		None Low Moderate High

Linear, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		(1180.9)
Larval Midgut		115.9
Larval Hindgut		594
Larval Malpighian Tubules		73.3
Larval Fat Body		3.3
Larval Salivary Gland		96.4
Larval Trachea		291.075
Larval Carcass		(4283.075)
Adult Head		60.7
Adult Eye		13.7
Adult Brain		25.9
Adult Thoracic-Abdominal Ganglion		14.3
Adult Crop		375
Adult Midgut		872
Adult Hindgut		281.8
Adult Malpighian Tubules		33.6
Adult Fat Body		435.2
Adult Salivary Gland		138.4
Adult Heart		375.325
Adult VirginFemale Spermatheca		600.5
Adult InseminatedFemale Spermatheca		370.7
Adult Ovary		1.9
Adult Testis		118.4
Adult Male Accessory Gland		28.8
Adult Carcass		183.2
Expression Level Scale		None Low Moderate High Very high

Linear, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		1180.9
Larval Midgut		115.9
Larval Hindgut		594
Larval Malpighian Tubules		73.3
Larval Fat Body		3.3
Larval Salivary Gland		96.4
Larval Trachea		291.075
Larval Carcass		4283.075
Adult Head		60.7
Adult Eye		13.7
Adult Brain		25.9
Adult Thoracic-Abdominal Ganglion		14.3
Adult Crop		375
Adult Midgut		872
Adult Hindgut		281.8
Adult Malpighian Tubules		33.6
Adult Fat Body		435.2
Adult Salivary Gland		138.4
Adult Heart		375.325
Adult VirginFemale Spermatheca		600.5
Adult InseminatedFemale Spermatheca		370.7
Adult Ovary		1.9
Adult Testis		118.4
Adult Male Accessory Gland		28.8
Adult Carcass		183.2
Expression Level Scale		Very high

log, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		1180.9
Larval Midgut		115.9
Larval Hindgut		594
Larval Malpighian Tubules		73.3
Larval Fat Body		3.3
Larval Salivary Gland		96.4
Larval Trachea		291.075
Larval Carcass		4283.075
Adult Head		60.7
Adult Eye		13.7
Adult Brain		25.9
Adult Thoracic-Abdominal Ganglion		14.3
Adult Crop		375
Adult Midgut		872
Adult Hindgut		281.8
Adult Malpighian Tubules		33.6
Adult Fat Body		435.2
Adult Salivary Gland		138.4
Adult Heart		375.325
Adult VirginFemale Spermatheca		600.5
Adult InseminatedFemale Spermatheca		370.7
Adult Ovary		1.9
Adult Testis		118.4
Adult Male Accessory Gland		28.8
Adult Carcass		183.2
Expression Level Scale		None Low Moderate High Very high

log, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		(1180.9)
Larval Midgut		115.9
Larval Hindgut		594
Larval Malpighian Tubules		73.3
Larval Fat Body		3.3
Larval Salivary Gland		96.4
Larval Trachea		291.075
Larval Carcass		(4283.075)
Adult Head		60.7
Adult Eye		13.7
Adult Brain		25.9
Adult Thoracic-Abdominal Ganglion		14.3
Adult Crop		375
Adult Midgut		(872)
Adult Hindgut		281.8
Adult Malpighian Tubules		33.6
Adult Fat Body		435.2
Adult Salivary Gland		138.4
Adult Heart		375.325
Adult VirginFemale Spermatheca		600.5
Adult InseminatedFemale Spermatheca		370.7
Adult Ovary		1.9
Adult Testis		118.4
Adult Male Accessory Gland		28.8
Adult Carcass		183.2
Expression Level Scale		None Low Moderate High

log, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		1180.9
Larval Midgut		115.9
Larval Hindgut		594
Larval Malpighian Tubules		73.3
Larval Fat Body		3.3
Larval Salivary Gland		96.4
Larval Trachea		291.075
Larval Carcass		(4283.075)
Adult Head		60.7
Adult Eye		13.7
Adult Brain		25.9
Adult Thoracic-Abdominal Ganglion		14.3
Adult Crop		375
Adult Midgut		872
Adult Hindgut		281.8
Adult Malpighian Tubules		33.6
Adult Fat Body		435.2
Adult Salivary Gland		138.4
Adult Heart		375.325
Adult VirginFemale Spermatheca		600.5
Adult InseminatedFemale Spermatheca		370.7
Adult Ovary		1.9
Adult Testis		118.4
Adult Male Accessory Gland		28.8
Adult Carcass		183.2
Expression Level Scale		None Low Moderate High Very high

log, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		1180.9
Larval Midgut		115.9
Larval Hindgut		594
Larval Malpighian Tubules		73.3
Larval Fat Body		3.3
Larval Salivary Gland		96.4
Larval Trachea		291.075
Larval Carcass		4283.075
Adult Head		60.7
Adult Eye		13.7
Adult Brain		25.9
Adult Thoracic-Abdominal Ganglion		14.3
Adult Crop		375
Adult Midgut		872
Adult Hindgut		281.8
Adult Malpighian Tubules		33.6
Adult Fat Body		435.2
Adult Salivary Gland		138.4
Adult Heart		375.325
Adult VirginFemale Spermatheca		600.5
Adult InseminatedFemale Spermatheca		370.7
Adult Ovary		1.9
Adult Testis		118.4
Adult Male Accessory Gland		28.8
Adult Carcass		183.2
Expression Level Scale		None Low Moderate High Very high

Heatmap
Tissue		Expression Level
Larval Central Nervous System
Larval Midgut
Larval Hindgut
Larval Malpighian Tubules
Larval Fat Body
Larval Salivary Gland
Larval Trachea
Larval Carcass
Adult Head
Adult Eye
Adult Brain
Adult Thoracic-Abdominal Ganglion
Adult Crop
Adult Midgut
Adult Hindgut
Adult Malpighian Tubules
Adult Fat Body
Adult Salivary Gland
Adult Heart
Adult VirginFemale Spermatheca
Adult InseminatedFemale Spermatheca
Adult Ovary
Adult Testis
Adult Male Accessory Gland
Adult Carcass

FlyAtlas Organ/Tissue Expression, larval vs. adult
Larval Expression Level	Tissue	Adult Expression Level
NA	Head	60.7
NA	Eye	no informative data
NA	Brain	no informative data
1180.9	Central Nervous System	NA
NA	Thoracic-Abdominal Ganglion	no informative data
NA	Crop	375
115.9	Midgut	872
594	Hindgut	281.8
73.3	Malpighian Tubules	no informative data
3.3	Fat Body	435.2
no informative data	Salivary Gland	no informative data
NA	Heart	375.325
291.075	Trachea	NA
NA	VirginFemale Spermatheca	600.5
NA	InseminatedFemale Spermatheca	370.7
NA	Ovary	1.9
NA	Testis	118.4
NA	Male Accessory Gland	no informative data
4283.075	Carcass	183.2

FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)

modENCODE Anatomy RNA-Seq

modENCODE Tissue Expression Data

(modENCODE_mRNA-Seq_tissues)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		15
central nervous system, larvae L3		13
central nervous system, pupae P8		6
head, virgin 1-day female		4
head, virgin 4-day female		4
head, virgin 20-day female		9
head, mated 1-day female		3
head, mated 4-day female		4
head, mated 20-day female		7
head, mated 1-day male		6
head, mated 4-day male		8
head, mated 20-day male		11
salivary gland, larvae L3 wandering		20
salivary gland, white prepupae		39
digestive system, larvae L3 wandering		9
digestive system, 1-day adult		46
digestive system, 4-day adult		28
digestive system, 20-day adult		57
fat body, larvae L3 wandering		1
fat body, white prepupae		4
fat body, pupae P8		7
carcass, larvae L3 wandering		76
carcass, 1-day adult		18
carcass, 4-day adult		15
carcass, 20-day adult		20
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		35
accessory gland, mated 4-day male		35
Expression Level Scale		Very low Low Moderate Moderately high High

Linear, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		15
central nervous system, larvae L3		13
central nervous system, pupae P8		6
head, virgin 1-day female		4
head, virgin 4-day female		4
head, virgin 20-day female		9
head, mated 1-day female		3
head, mated 4-day female		4
head, mated 20-day female		7
head, mated 1-day male		6
head, mated 4-day male		8
head, mated 20-day male		11
salivary gland, larvae L3 wandering		20
salivary gland, white prepupae		(39)
digestive system, larvae L3 wandering		9
digestive system, 1-day adult		(46)
digestive system, 4-day adult		(28)
digestive system, 20-day adult		(57)
fat body, larvae L3 wandering		1
fat body, white prepupae		4
fat body, pupae P8		7
carcass, larvae L3 wandering		(76)
carcass, 1-day adult		18
carcass, 4-day adult		15
carcass, 20-day adult		20
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		(35)
accessory gland, mated 4-day male		(35)
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		15
central nervous system, larvae L3		13
central nervous system, pupae P8		6
head, virgin 1-day female		4
head, virgin 4-day female		4
head, virgin 20-day female		9
head, mated 1-day female		3
head, mated 4-day female		4
head, mated 20-day female		7
head, mated 1-day male		6
head, mated 4-day male		8
head, mated 20-day male		11
salivary gland, larvae L3 wandering		20
salivary gland, white prepupae		39
digestive system, larvae L3 wandering		9
digestive system, 1-day adult		46
digestive system, 4-day adult		28
digestive system, 20-day adult		57
fat body, larvae L3 wandering		1
fat body, white prepupae		4
fat body, pupae P8		7
carcass, larvae L3 wandering		76
carcass, 1-day adult		18
carcass, 4-day adult		15
carcass, 20-day adult		20
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		35
accessory gland, mated 4-day male		35
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		15
central nervous system, larvae L3		13
central nervous system, pupae P8		6
head, virgin 1-day female		4
head, virgin 4-day female		4
head, virgin 20-day female		9
head, mated 1-day female		3
head, mated 4-day female		4
head, mated 20-day female		7
head, mated 1-day male		6
head, mated 4-day male		8
head, mated 20-day male		11
salivary gland, larvae L3 wandering		20
salivary gland, white prepupae		39
digestive system, larvae L3 wandering		9
digestive system, 1-day adult		46
digestive system, 4-day adult		28
digestive system, 20-day adult		57
fat body, larvae L3 wandering		1
fat body, white prepupae		4
fat body, pupae P8		7
carcass, larvae L3 wandering		76
carcass, 1-day adult		18
carcass, 4-day adult		15
carcass, 20-day adult		20
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		35
accessory gland, mated 4-day male		35
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		15
central nervous system, larvae L3		13
central nervous system, pupae P8		6
head, virgin 1-day female		4
head, virgin 4-day female		4
head, virgin 20-day female		9
head, mated 1-day female		3
head, mated 4-day female		4
head, mated 20-day female		7
head, mated 1-day male		6
head, mated 4-day male		8
head, mated 20-day male		11
salivary gland, larvae L3 wandering		20
salivary gland, white prepupae		39
digestive system, larvae L3 wandering		9
digestive system, 1-day adult		46
digestive system, 4-day adult		28
digestive system, 20-day adult		57
fat body, larvae L3 wandering		1
fat body, white prepupae		4
fat body, pupae P8		7
carcass, larvae L3 wandering		76
carcass, 1-day adult		18
carcass, 4-day adult		15
carcass, 20-day adult		20
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		35
accessory gland, mated 4-day male		35
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		15
central nervous system, larvae L3		13
central nervous system, pupae P8		6
head, virgin 1-day female		4
head, virgin 4-day female		4
head, virgin 20-day female		9
head, mated 1-day female		3
head, mated 4-day female		4
head, mated 20-day female		7
head, mated 1-day male		6
head, mated 4-day male		8
head, mated 20-day male		11
salivary gland, larvae L3 wandering		20
salivary gland, white prepupae		(39)
digestive system, larvae L3 wandering		9
digestive system, 1-day adult		(46)
digestive system, 4-day adult		28
digestive system, 20-day adult		(57)
fat body, larvae L3 wandering		1
fat body, white prepupae		4
fat body, pupae P8		7
carcass, larvae L3 wandering		(76)
carcass, 1-day adult		18
carcass, 4-day adult		15
carcass, 20-day adult		20
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		(35)
accessory gland, mated 4-day male		(35)
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		15
central nervous system, larvae L3		13
central nervous system, pupae P8		6
head, virgin 1-day female		4
head, virgin 4-day female		4
head, virgin 20-day female		9
head, mated 1-day female		3
head, mated 4-day female		4
head, mated 20-day female		7
head, mated 1-day male		6
head, mated 4-day male		8
head, mated 20-day male		11
salivary gland, larvae L3 wandering		20
salivary gland, white prepupae		39
digestive system, larvae L3 wandering		9
digestive system, 1-day adult		46
digestive system, 4-day adult		28
digestive system, 20-day adult		57
fat body, larvae L3 wandering		1
fat body, white prepupae		4
fat body, pupae P8		7
carcass, larvae L3 wandering		76
carcass, 1-day adult		18
carcass, 4-day adult		15
carcass, 20-day adult		20
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		35
accessory gland, mated 4-day male		35
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		15
central nervous system, larvae L3		13
central nervous system, pupae P8		6
head, virgin 1-day female		4
head, virgin 4-day female		4
head, virgin 20-day female		9
head, mated 1-day female		3
head, mated 4-day female		4
head, mated 20-day female		7
head, mated 1-day male		6
head, mated 4-day male		8
head, mated 20-day male		11
salivary gland, larvae L3 wandering		20
salivary gland, white prepupae		39
digestive system, larvae L3 wandering		9
digestive system, 1-day adult		46
digestive system, 4-day adult		28
digestive system, 20-day adult		57
fat body, larvae L3 wandering		1
fat body, white prepupae		4
fat body, pupae P8		7
carcass, larvae L3 wandering		76
carcass, 1-day adult		18
carcass, 4-day adult		15
carcass, 20-day adult		20
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		35
accessory gland, mated 4-day male		35
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Tissue		Expression Level
imaginal disc, larvae L3 wandering
central nervous system, larvae L3
central nervous system, pupae P8
head, virgin 1-day female
head, virgin 4-day female
head, virgin 20-day female
head, mated 1-day female
head, mated 4-day female
head, mated 20-day female
head, mated 1-day male
head, mated 4-day male
head, mated 20-day male
salivary gland, larvae L3 wandering
salivary gland, white prepupae
digestive system, larvae L3 wandering
digestive system, 1-day adult
digestive system, 4-day adult
digestive system, 20-day adult
fat body, larvae L3 wandering
fat body, white prepupae
fat body, pupae P8
carcass, larvae L3 wandering
carcass, 1-day adult
carcass, 4-day adult
carcass, 20-day adult
ovary, virgin 4-day female
ovary, mated 4-day female
testis, mated 4-day male
accessory gland, mated 4-day male

modENCODE Development RNA-Seq

modENCODE Temporal Expression Data

(modENCODE_mRNA-Seq_U)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of low expression. Peak expression observed within 12-24 hour embryonic stages, at stages throughout the larval period.
[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		2
embryo 02-04hr		10
embryo 04-06hr		8
embryo 06-08hr		23
embryo 08-10hr		22
embryo 10-12hr		89
embryo 12-14hr		154
embryo 14-16hr		420
embryo 16-18hr		256
embryo 18-20hr		167
embryo 20-22hr		271
embryo 22-24hr		235
larva L1		462
larva L2		430
larva L3 12hr old		335
larva L3 puffstage 1-2		331
larva L3 puffstage 3-6		150
larva L3 puffstage 7-9		59
white prepupae new		23
white prepupae 12hr		27
white prepupae 24hr		22
pupae 2d postWPP		47
pupae 3d postWPP		26
pupae 4d postWPP		55
adult male 01day		42
adult male 05day		37
adult male 30day		32
adult female 01day		33
adult female 05day		9
adult female 30day		8
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		2
embryo 02-04hr		10
embryo 04-06hr		8
embryo 06-08hr		23
embryo 08-10hr		22
embryo 10-12hr		(89)
embryo 12-14hr		(154)
embryo 14-16hr		(420)
embryo 16-18hr		(256)
embryo 18-20hr		(167)
embryo 20-22hr		(271)
embryo 22-24hr		(235)
larva L1		(462)
larva L2		(430)
larva L3 12hr old		(335)
larva L3 puffstage 1-2		(331)
larva L3 puffstage 3-6		(150)
larva L3 puffstage 7-9		(59)
white prepupae new		23
white prepupae 12hr		(27)
white prepupae 24hr		22
pupae 2d postWPP		(47)
pupae 3d postWPP		26
pupae 4d postWPP		(55)
adult male 01day		(42)
adult male 05day		(37)
adult male 30day		(32)
adult female 01day		(33)
adult female 05day		9
adult female 30day		8
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		2
embryo 02-04hr		10
embryo 04-06hr		8
embryo 06-08hr		23
embryo 08-10hr		22
embryo 10-12hr		89
embryo 12-14hr		(154)
embryo 14-16hr		(420)
embryo 16-18hr		(256)
embryo 18-20hr		(167)
embryo 20-22hr		(271)
embryo 22-24hr		(235)
larva L1		(462)
larva L2		(430)
larva L3 12hr old		(335)
larva L3 puffstage 1-2		(331)
larva L3 puffstage 3-6		(150)
larva L3 puffstage 7-9		59
white prepupae new		23
white prepupae 12hr		27
white prepupae 24hr		22
pupae 2d postWPP		47
pupae 3d postWPP		26
pupae 4d postWPP		55
adult male 01day		42
adult male 05day		37
adult male 30day		32
adult female 01day		33
adult female 05day		9
adult female 30day		8
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		2
embryo 02-04hr		10
embryo 04-06hr		8
embryo 06-08hr		23
embryo 08-10hr		22
embryo 10-12hr		89
embryo 12-14hr		154
embryo 14-16hr		420
embryo 16-18hr		256
embryo 18-20hr		167
embryo 20-22hr		271
embryo 22-24hr		235
larva L1		462
larva L2		430
larva L3 12hr old		335
larva L3 puffstage 1-2		331
larva L3 puffstage 3-6		150
larva L3 puffstage 7-9		59
white prepupae new		23
white prepupae 12hr		27
white prepupae 24hr		22
pupae 2d postWPP		47
pupae 3d postWPP		26
pupae 4d postWPP		55
adult male 01day		42
adult male 05day		37
adult male 30day		32
adult female 01day		33
adult female 05day		9
adult female 30day		8
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		2
embryo 02-04hr		10
embryo 04-06hr		8
embryo 06-08hr		23
embryo 08-10hr		22
embryo 10-12hr		89
embryo 12-14hr		154
embryo 14-16hr		420
embryo 16-18hr		256
embryo 18-20hr		167
embryo 20-22hr		271
embryo 22-24hr		235
larva L1		462
larva L2		430
larva L3 12hr old		335
larva L3 puffstage 1-2		331
larva L3 puffstage 3-6		150
larva L3 puffstage 7-9		59
white prepupae new		23
white prepupae 12hr		27
white prepupae 24hr		22
pupae 2d postWPP		47
pupae 3d postWPP		26
pupae 4d postWPP		55
adult male 01day		42
adult male 05day		37
adult male 30day		32
adult female 01day		33
adult female 05day		9
adult female 30day		8
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		2
embryo 02-04hr		10
embryo 04-06hr		8
embryo 06-08hr		23
embryo 08-10hr		22
embryo 10-12hr		(89)
embryo 12-14hr		(154)
embryo 14-16hr		(420)
embryo 16-18hr		(256)
embryo 18-20hr		(167)
embryo 20-22hr		(271)
embryo 22-24hr		(235)
larva L1		(462)
larva L2		(430)
larva L3 12hr old		(335)
larva L3 puffstage 1-2		(331)
larva L3 puffstage 3-6		(150)
larva L3 puffstage 7-9		(59)
white prepupae new		23
white prepupae 12hr		27
white prepupae 24hr		22
pupae 2d postWPP		(47)
pupae 3d postWPP		26
pupae 4d postWPP		(55)
adult male 01day		(42)
adult male 05day		(37)
adult male 30day		32
adult female 01day		(33)
adult female 05day		9
adult female 30day		8
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		2
embryo 02-04hr		10
embryo 04-06hr		8
embryo 06-08hr		23
embryo 08-10hr		22
embryo 10-12hr		89
embryo 12-14hr		(154)
embryo 14-16hr		(420)
embryo 16-18hr		(256)
embryo 18-20hr		(167)
embryo 20-22hr		(271)
embryo 22-24hr		(235)
larva L1		(462)
larva L2		(430)
larva L3 12hr old		(335)
larva L3 puffstage 1-2		(331)
larva L3 puffstage 3-6		(150)
larva L3 puffstage 7-9		59
white prepupae new		23
white prepupae 12hr		27
white prepupae 24hr		22
pupae 2d postWPP		47
pupae 3d postWPP		26
pupae 4d postWPP		55
adult male 01day		42
adult male 05day		37
adult male 30day		32
adult female 01day		33
adult female 05day		9
adult female 30day		8
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		2
embryo 02-04hr		10
embryo 04-06hr		8
embryo 06-08hr		23
embryo 08-10hr		22
embryo 10-12hr		89
embryo 12-14hr		154
embryo 14-16hr		420
embryo 16-18hr		256
embryo 18-20hr		167
embryo 20-22hr		271
embryo 22-24hr		235
larva L1		462
larva L2		430
larva L3 12hr old		335
larva L3 puffstage 1-2		331
larva L3 puffstage 3-6		150
larva L3 puffstage 7-9		59
white prepupae new		23
white prepupae 12hr		27
white prepupae 24hr		22
pupae 2d postWPP		47
pupae 3d postWPP		26
pupae 4d postWPP		55
adult male 01day		42
adult male 05day		37
adult male 30day		32
adult female 01day		33
adult female 05day		9
adult female 30day		8
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Developmental Stage		Expression Level
embryo 00-02hr
embryo 02-04hr
embryo 04-06hr
embryo 06-08hr
embryo 08-10hr
embryo 10-12hr
embryo 12-14hr
embryo 14-16hr
embryo 16-18hr
embryo 18-20hr
embryo 20-22hr
embryo 22-24hr
larva L1
larva L2
larva L3 12hr old
larva L3 puffstage 1-2
larva L3 puffstage 3-6
larva L3 puffstage 7-9
white prepupae new
white prepupae 12hr
white prepupae 24hr
pupae 2d postWPP
pupae 3d postWPP
pupae 4d postWPP
adult male 01day
adult male 05day
adult male 30day
adult female 01day
adult female 05day
adult female 30day

modENCODE Temporal Expression Data (Graveley et al., 2011)

modENCODE Cell Lines RNA-Seq

modENCODE Cell Line Expression Data

(modENCODE_mRNA-Seq_cell.A)

(modENCODE_mRNA-Seq_cell.B)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		56
Schneider line 2 Sg4		52
embryonic 1182-4H		34
embryonic GM2		107
embryonic Kc167		173
embryonic S1		188
embryonic S3		13
leg disc CME L1		262
wing disc CME-W2		90
wing disc ML-DmD8		213
wing disc ML-DmD9		249
wing disc ML-DmD16-c3		18
wing disc ML-DmD21		22
wing disc ML-DmD32		36
haltere disc ML-DmD17-c3		6
eye-antennal disc ML-DmD11		35
antennal disc ML-DmD20-c5		41
mixed discs ML-DmD4-c1		18
CNS ML-DmBG1-c1		237
CNS ML-DmBG2-c2		83
tumorous blood cells mbn2		285
ovary fGS/OSS		200
ovary OSC		616
ovary OSS		28
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		(56)
Schneider line 2 Sg4		(52)
embryonic 1182-4H		(34)
embryonic GM2		(107)
embryonic Kc167		(173)
embryonic S1		(188)
embryonic S3		13
leg disc CME L1		(262)
wing disc CME-W2		(90)
wing disc ML-DmD8		(213)
wing disc ML-DmD9		(249)
wing disc ML-DmD16-c3		18
wing disc ML-DmD21		22
wing disc ML-DmD32		(36)
haltere disc ML-DmD17-c3		6
eye-antennal disc ML-DmD11		(35)
antennal disc ML-DmD20-c5		(41)
mixed discs ML-DmD4-c1		18
CNS ML-DmBG1-c1		(237)
CNS ML-DmBG2-c2		(83)
tumorous blood cells mbn2		(285)
ovary fGS/OSS		(200)
ovary OSC		(616)
ovary OSS		(28)
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		56
Schneider line 2 Sg4		52
embryonic 1182-4H		34
embryonic GM2		107
embryonic Kc167		(173)
embryonic S1		(188)
embryonic S3		13
leg disc CME L1		(262)
wing disc CME-W2		90
wing disc ML-DmD8		(213)
wing disc ML-DmD9		(249)
wing disc ML-DmD16-c3		18
wing disc ML-DmD21		22
wing disc ML-DmD32		36
haltere disc ML-DmD17-c3		6
eye-antennal disc ML-DmD11		35
antennal disc ML-DmD20-c5		41
mixed discs ML-DmD4-c1		18
CNS ML-DmBG1-c1		(237)
CNS ML-DmBG2-c2		83
tumorous blood cells mbn2		(285)
ovary fGS/OSS		(200)
ovary OSC		(616)
ovary OSS		28
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		56
Schneider line 2 Sg4		52
embryonic 1182-4H		34
embryonic GM2		107
embryonic Kc167		173
embryonic S1		188
embryonic S3		13
leg disc CME L1		262
wing disc CME-W2		90
wing disc ML-DmD8		213
wing disc ML-DmD9		249
wing disc ML-DmD16-c3		18
wing disc ML-DmD21		22
wing disc ML-DmD32		36
haltere disc ML-DmD17-c3		6
eye-antennal disc ML-DmD11		35
antennal disc ML-DmD20-c5		41
mixed discs ML-DmD4-c1		18
CNS ML-DmBG1-c1		237
CNS ML-DmBG2-c2		83
tumorous blood cells mbn2		285
ovary fGS/OSS		200
ovary OSC		616
ovary OSS		28
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		56
Schneider line 2 Sg4		52
embryonic 1182-4H		34
embryonic GM2		107
embryonic Kc167		173
embryonic S1		188
embryonic S3		13
leg disc CME L1		262
wing disc CME-W2		90
wing disc ML-DmD8		213
wing disc ML-DmD9		249
wing disc ML-DmD16-c3		18
wing disc ML-DmD21		22
wing disc ML-DmD32		36
haltere disc ML-DmD17-c3		6
eye-antennal disc ML-DmD11		35
antennal disc ML-DmD20-c5		41
mixed discs ML-DmD4-c1		18
CNS ML-DmBG1-c1		237
CNS ML-DmBG2-c2		83
tumorous blood cells mbn2		285
ovary fGS/OSS		200
ovary OSC		616
ovary OSS		28
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

log, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		(56)
Schneider line 2 Sg4		(52)
embryonic 1182-4H		(34)
embryonic GM2		(107)
embryonic Kc167		(173)
embryonic S1		(188)
embryonic S3		13
leg disc CME L1		(262)
wing disc CME-W2		(90)
wing disc ML-DmD8		(213)
wing disc ML-DmD9		(249)
wing disc ML-DmD16-c3		18
wing disc ML-DmD21		22
wing disc ML-DmD32		(36)
haltere disc ML-DmD17-c3		6
eye-antennal disc ML-DmD11		(35)
antennal disc ML-DmD20-c5		(41)
mixed discs ML-DmD4-c1		18
CNS ML-DmBG1-c1		(237)
CNS ML-DmBG2-c2		(83)
tumorous blood cells mbn2		(285)
ovary fGS/OSS		(200)
ovary OSC		(616)
ovary OSS		28
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		56
Schneider line 2 Sg4		52
embryonic 1182-4H		34
embryonic GM2		107
embryonic Kc167		(173)
embryonic S1		(188)
embryonic S3		13
leg disc CME L1		(262)
wing disc CME-W2		90
wing disc ML-DmD8		(213)
wing disc ML-DmD9		(249)
wing disc ML-DmD16-c3		18
wing disc ML-DmD21		22
wing disc ML-DmD32		36
haltere disc ML-DmD17-c3		6
eye-antennal disc ML-DmD11		35
antennal disc ML-DmD20-c5		41
mixed discs ML-DmD4-c1		18
CNS ML-DmBG1-c1		(237)
CNS ML-DmBG2-c2		83
tumorous blood cells mbn2		(285)
ovary fGS/OSS		(200)
ovary OSC		(616)
ovary OSS		28
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		56
Schneider line 2 Sg4		52
embryonic 1182-4H		34
embryonic GM2		107
embryonic Kc167		173
embryonic S1		188
embryonic S3		13
leg disc CME L1		262
wing disc CME-W2		90
wing disc ML-DmD8		213
wing disc ML-DmD9		249
wing disc ML-DmD16-c3		18
wing disc ML-DmD21		22
wing disc ML-DmD32		36
haltere disc ML-DmD17-c3		6
eye-antennal disc ML-DmD11		35
antennal disc ML-DmD20-c5		41
mixed discs ML-DmD4-c1		18
CNS ML-DmBG1-c1		237
CNS ML-DmBG2-c2		83
tumorous blood cells mbn2		285
ovary fGS/OSS		200
ovary OSC		616
ovary OSS		28
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Cell Line		Expression Level
Schneider line 2 S2R+
Schneider line 2 Sg4
embryonic 1182-4H
embryonic GM2
embryonic Kc167
embryonic S1
embryonic S3
leg disc CME L1
wing disc CME-W2
wing disc ML-DmD8
wing disc ML-DmD9
wing disc ML-DmD16-c3
wing disc ML-DmD21
wing disc ML-DmD32
haltere disc ML-DmD17-c3
eye-antennal disc ML-DmD11
antennal disc ML-DmD20-c5
mixed discs ML-DmD4-c1
CNS ML-DmBG1-c1
CNS ML-DmBG2-c2
tumorous blood cells mbn2
ovary fGS/OSS
ovary OSC
ovary OSS

modENCODE Treatments RNA-Seq

modENCODE Treatment Expression Data

(modENCODE_mRNA-Seq_treatments)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		11
cold shock, 4-day adult		8
heat shock, 4-day adult		178
Cadmium 50 mM 6 hrs, larvae L3		316
Cadmium 50 mM 12 hrs, larvae L3		201
Cadmium 50 mM 48 hrs, 4-day adult		220
Cadmium 100 mM 48 hrs, 4-day adult		44
Copper 0.5 mM 12 hrs, larvae L3		268
Copper 15 mM 48 hrs, 4-day adult		29
Zinc 5 mM 12 hrs, larvae L3		193
Zinc 4.5 mM 48 hrs, 4-day adult		18
Ethanol 2.5% 3 hrs, larvae L3		25
Ethanol 5% 3 hrs, larvae L3		291
Ethanol 10% 3 hrs, larvae L3		391
Caffeine 1.5 mg/ml 4 hrs, larvae L3		236
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		12
Caffeine 25 mg/ml 48 hrs, 4-day adult		17
Paraquat 5 mM 48 hrs, 4-day adult		8
Paraquat 10 mM 48 hrs, 4-day adult		178
Rotenone 2 μg 12 hrs, larvae L3		275
Rotenone 8 μg 12 hrs, larvae L3		462
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		11
cold shock, 4-day adult		8
heat shock, 4-day adult		(178)
Cadmium 50 mM 6 hrs, larvae L3		(316)
Cadmium 50 mM 12 hrs, larvae L3		(201)
Cadmium 50 mM 48 hrs, 4-day adult		(220)
Cadmium 100 mM 48 hrs, 4-day adult		(44)
Copper 0.5 mM 12 hrs, larvae L3		(268)
Copper 15 mM 48 hrs, 4-day adult		(29)
Zinc 5 mM 12 hrs, larvae L3		(193)
Zinc 4.5 mM 48 hrs, 4-day adult		18
Ethanol 2.5% 3 hrs, larvae L3		25
Ethanol 5% 3 hrs, larvae L3		(291)
Ethanol 10% 3 hrs, larvae L3		(391)
Caffeine 1.5 mg/ml 4 hrs, larvae L3		(236)
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		12
Caffeine 25 mg/ml 48 hrs, 4-day adult		17
Paraquat 5 mM 48 hrs, 4-day adult		8
Paraquat 10 mM 48 hrs, 4-day adult		(178)
Rotenone 2 μg 12 hrs, larvae L3		(275)
Rotenone 8 μg 12 hrs, larvae L3		(462)
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		11
cold shock, 4-day adult		8
heat shock, 4-day adult		(178)
Cadmium 50 mM 6 hrs, larvae L3		(316)
Cadmium 50 mM 12 hrs, larvae L3		(201)
Cadmium 50 mM 48 hrs, 4-day adult		(220)
Cadmium 100 mM 48 hrs, 4-day adult		44
Copper 0.5 mM 12 hrs, larvae L3		(268)
Copper 15 mM 48 hrs, 4-day adult		29
Zinc 5 mM 12 hrs, larvae L3		(193)
Zinc 4.5 mM 48 hrs, 4-day adult		18
Ethanol 2.5% 3 hrs, larvae L3		25
Ethanol 5% 3 hrs, larvae L3		(291)
Ethanol 10% 3 hrs, larvae L3		(391)
Caffeine 1.5 mg/ml 4 hrs, larvae L3		(236)
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		12
Caffeine 25 mg/ml 48 hrs, 4-day adult		17
Paraquat 5 mM 48 hrs, 4-day adult		8
Paraquat 10 mM 48 hrs, 4-day adult		(178)
Rotenone 2 μg 12 hrs, larvae L3		(275)
Rotenone 8 μg 12 hrs, larvae L3		(462)
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		11
cold shock, 4-day adult		8
heat shock, 4-day adult		178
Cadmium 50 mM 6 hrs, larvae L3		316
Cadmium 50 mM 12 hrs, larvae L3		201
Cadmium 50 mM 48 hrs, 4-day adult		220
Cadmium 100 mM 48 hrs, 4-day adult		44
Copper 0.5 mM 12 hrs, larvae L3		268
Copper 15 mM 48 hrs, 4-day adult		29
Zinc 5 mM 12 hrs, larvae L3		193
Zinc 4.5 mM 48 hrs, 4-day adult		18
Ethanol 2.5% 3 hrs, larvae L3		25
Ethanol 5% 3 hrs, larvae L3		291
Ethanol 10% 3 hrs, larvae L3		391
Caffeine 1.5 mg/ml 4 hrs, larvae L3		236
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		12
Caffeine 25 mg/ml 48 hrs, 4-day adult		17
Paraquat 5 mM 48 hrs, 4-day adult		8
Paraquat 10 mM 48 hrs, 4-day adult		178
Rotenone 2 μg 12 hrs, larvae L3		275
Rotenone 8 μg 12 hrs, larvae L3		462
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		11
cold shock, 4-day adult		8
heat shock, 4-day adult		178
Cadmium 50 mM 6 hrs, larvae L3		316
Cadmium 50 mM 12 hrs, larvae L3		201
Cadmium 50 mM 48 hrs, 4-day adult		220
Cadmium 100 mM 48 hrs, 4-day adult		44
Copper 0.5 mM 12 hrs, larvae L3		268
Copper 15 mM 48 hrs, 4-day adult		29
Zinc 5 mM 12 hrs, larvae L3		193
Zinc 4.5 mM 48 hrs, 4-day adult		18
Ethanol 2.5% 3 hrs, larvae L3		25
Ethanol 5% 3 hrs, larvae L3		291
Ethanol 10% 3 hrs, larvae L3		391
Caffeine 1.5 mg/ml 4 hrs, larvae L3		236
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		12
Caffeine 25 mg/ml 48 hrs, 4-day adult		17
Paraquat 5 mM 48 hrs, 4-day adult		8
Paraquat 10 mM 48 hrs, 4-day adult		178
Rotenone 2 μg 12 hrs, larvae L3		275
Rotenone 8 μg 12 hrs, larvae L3		462
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		11
cold shock, 4-day adult		8
heat shock, 4-day adult		(178)
Cadmium 50 mM 6 hrs, larvae L3		(316)
Cadmium 50 mM 12 hrs, larvae L3		(201)
Cadmium 50 mM 48 hrs, 4-day adult		(220)
Cadmium 100 mM 48 hrs, 4-day adult		(44)
Copper 0.5 mM 12 hrs, larvae L3		(268)
Copper 15 mM 48 hrs, 4-day adult		29
Zinc 5 mM 12 hrs, larvae L3		(193)
Zinc 4.5 mM 48 hrs, 4-day adult		18
Ethanol 2.5% 3 hrs, larvae L3		25
Ethanol 5% 3 hrs, larvae L3		(291)
Ethanol 10% 3 hrs, larvae L3		(391)
Caffeine 1.5 mg/ml 4 hrs, larvae L3		(236)
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		12
Caffeine 25 mg/ml 48 hrs, 4-day adult		17
Paraquat 5 mM 48 hrs, 4-day adult		8
Paraquat 10 mM 48 hrs, 4-day adult		(178)
Rotenone 2 μg 12 hrs, larvae L3		(275)
Rotenone 8 μg 12 hrs, larvae L3		(462)
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		11
cold shock, 4-day adult		8
heat shock, 4-day adult		(178)
Cadmium 50 mM 6 hrs, larvae L3		(316)
Cadmium 50 mM 12 hrs, larvae L3		(201)
Cadmium 50 mM 48 hrs, 4-day adult		(220)
Cadmium 100 mM 48 hrs, 4-day adult		44
Copper 0.5 mM 12 hrs, larvae L3		(268)
Copper 15 mM 48 hrs, 4-day adult		29
Zinc 5 mM 12 hrs, larvae L3		(193)
Zinc 4.5 mM 48 hrs, 4-day adult		18
Ethanol 2.5% 3 hrs, larvae L3		25
Ethanol 5% 3 hrs, larvae L3		(291)
Ethanol 10% 3 hrs, larvae L3		(391)
Caffeine 1.5 mg/ml 4 hrs, larvae L3		(236)
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		12
Caffeine 25 mg/ml 48 hrs, 4-day adult		17
Paraquat 5 mM 48 hrs, 4-day adult		8
Paraquat 10 mM 48 hrs, 4-day adult		(178)
Rotenone 2 μg 12 hrs, larvae L3		(275)
Rotenone 8 μg 12 hrs, larvae L3		(462)
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		11
cold shock, 4-day adult		8
heat shock, 4-day adult		178
Cadmium 50 mM 6 hrs, larvae L3		316
Cadmium 50 mM 12 hrs, larvae L3		201
Cadmium 50 mM 48 hrs, 4-day adult		220
Cadmium 100 mM 48 hrs, 4-day adult		44
Copper 0.5 mM 12 hrs, larvae L3		268
Copper 15 mM 48 hrs, 4-day adult		29
Zinc 5 mM 12 hrs, larvae L3		193
Zinc 4.5 mM 48 hrs, 4-day adult		18
Ethanol 2.5% 3 hrs, larvae L3		25
Ethanol 5% 3 hrs, larvae L3		291
Ethanol 10% 3 hrs, larvae L3		391
Caffeine 1.5 mg/ml 4 hrs, larvae L3		236
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		12
Caffeine 25 mg/ml 48 hrs, 4-day adult		17
Paraquat 5 mM 48 hrs, 4-day adult		8
Paraquat 10 mM 48 hrs, 4-day adult		178
Rotenone 2 μg 12 hrs, larvae L3		275
Rotenone 8 μg 12 hrs, larvae L3		462
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Treatment		Expression Level
extended cold, 4-day adult
cold shock, 4-day adult
heat shock, 4-day adult
Cadmium 50 mM 6 hrs, larvae L3
Cadmium 50 mM 12 hrs, larvae L3
Cadmium 50 mM 48 hrs, 4-day adult
Cadmium 100 mM 48 hrs, 4-day adult
Copper 0.5 mM 12 hrs, larvae L3
Copper 15 mM 48 hrs, 4-day adult
Zinc 5 mM 12 hrs, larvae L3
Zinc 4.5 mM 48 hrs, 4-day adult
Ethanol 2.5% 3 hrs, larvae L3
Ethanol 5% 3 hrs, larvae L3
Ethanol 10% 3 hrs, larvae L3
Caffeine 1.5 mg/ml 4 hrs, larvae L3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
Caffeine 25 mg/ml 48 hrs, 4-day adult
Paraquat 5 mM 48 hrs, 4-day adult
Paraquat 10 mM 48 hrs, 4-day adult
Rotenone 2 μg 12 hrs, larvae L3
Rotenone 8 μg 12 hrs, larvae L3

Expression Clusters

A cluster of genes with similar mRNA expression dynamics across development.

(The modENCODE Consortium, 2010)

mE2_34_mRNA_expression_cluster_09

External Data & Images

Linkouts

BDGP expression data

•

PC00103

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0001258

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG10160-RA

FlyExpress - Embryonic expression images (BDGP data)

•

Show all FlyExpress processed images for ImpL3

Stages(s) 4-6

Stages(s) 13-16

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

viable, with Scer\GAL4^pnr-MD237

ImpL3^GD6887

Phenotype manifest in

Allele

Classical Alleles ( 0 )

For All Classical Alleles Show

Allele of ImpL3	Class	Mutagen	Stocks	Known lesion

Alleles Carried on Transgenic Constructs ( 4 )

For All Alleles Carried on Transgenic Constructs Show

Allele of ImpL3	Mutagen	Stocks	Known lesion
ImpL3^GD6887	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
ImpL3^HMS00039	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
ImpL3^KK102330	in vitro construct - RNAi in vitro construct - regulatory fusion	0	Yes
ImpL3^NIG.10160R	in vitro construct - RNAi in vitro construct - regulatory fusion	0	Yes