General Information
Symbol
Dmel\in
Species
D. melanogaster
Name
inturned
Annotation Symbol
CG16993
Feature Type
FlyBase ID
FBgn0001259
Gene Model Status
Stock Availability
Gene Snapshot
Inturned (In) is a cytoplasmic protein that functions as a downstream member of the fz-stan Planar Cell Polarity pathway that controls hair and bristle polarity in the adult. In directly binds with fy, frtz and mwh and accumulates on the proximal side of wing cells. [Date last reviewed: 2016-06-23]
Genomic Location
Cytogenetic map
Sequence location
3L:20,367,198..20,370,259 [+]
Recombination map
3-47
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
GO Summary Ribbons
Families, Domains and Molecular Function
Gene Group Membership (FlyBase)
Protein Family (UniProt, Sequence Similarities)
Belongs to the inturned family. (Q9VPH0)
Protein Domains/Motifs
Molecular Function (see GO section for details)
Experimental Evidence
-
Predictions / Assertions
-
Summaries
UniProt Contributed Function Data
Plays a role in the definition of cell polarity via the planar cell polarity (PCP) cascade. Acts downstream of fuz and accumulates asymmetrically in wing cells to regulate planar polarity in the wing. Probably acts by regulating ciliogenesis.
(UniProt, Q9VPH0)
Phenotypic Description from the Red Book (Lindsley and Zimm 1992)
in: inturned
Hairs and bristles on thorax and abdominal tergites, directed irregularly toward midline; trichomes and chaetae on mesonotum coordinately misdirected (Toney and Thompson, 1980, Experientia 36: 344-45). Marginal hairs of wing stand out from wing margin; trichomes on wing blade no longer directed in parallel with longitudinal veins; frequently divergent therefrom. 50-60% of trichome-bearing cells express duplicated or rarely triplicated trichomes. Wings slightly spread and tend to be long and narrow. Somatic clones of homozygous cells in wing described by Gubb and Garcia-Bellido. Incomplete extra leg joints tend to form as mirror-image duplications proximal to the normal joints on tarsal segments three and four (Held, Duarte, and Derakhshanian, 1986, Wilhelm Roux's Arch. Dev. Biol. 195: 145-57). RK1.
Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the GBrowse view of Dmel\in or the JBrowse view of Dmel\in for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model
Gene model reviewed during 5.39
Gene model reviewed during 5.45
Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0078183
3062
869
Additional Transcript Data and Comments
Reported size (kB)
3.5 (northern blot)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0077841
98.1
869
6.16
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Subunit Structure (UniProtKB)
Interacts with mwh.
(UniProt, Q9VPH0)
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\in using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Gene Ontology (5 terms)
Molecular Function (0 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (0 terms)
Biological Process (4 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
inferred from mutant phenotype
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
inferred from electronic annotation with InterPro:IPR039151
(assigned by InterPro )
inferred from electronic annotation with InterPro:IPR039151
(assigned by InterPro )
traceable author statement
Cellular Component (1 term)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
inferred from sequence or structural similarity
Expression Data
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
antennal anlage

Comment: reported as procephalic ectoderm anlage

central brain anlage

Comment: reported as procephalic ectoderm anlage

dorsal head epidermis anlage

Comment: reported as procephalic ectoderm anlage

visual anlage

Comment: reported as procephalic ectoderm anlage

antennal primordium

Comment: reported as procephalic ectoderm primordium

central brain primordium

Comment: reported as procephalic ectoderm primordium

visual primordium

Comment: reported as procephalic ectoderm primordium

dorsal head epidermis primordium

Comment: reported as procephalic ectoderm primordium

lateral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

ventral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\in in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 7-8
  • Stages(s) 11-12
  • Stages(s) 13-16
Alleles, Insertions, Transgenic Constructs and Phenotypes
Classical and Insertion Alleles ( 31 )
For All Classical and Insertion Alleles Show
 
Allele of in
Class
Mutagen
Associated Insertion
Stocks
Known lesion
    0
    --
      0
      --
      Other relevant insertions
      miscellaneous insertions
      Name
      Expression Data
      Transgenic Constructs ( 13 )
      For All Alleles Carried on Transgenic Constructs Show
      Transgenic constructs containing/affecting coding region of in
      Allele of in
      Mutagen
      Associated Transgenic Construct
      Stocks
      Transgenic constructs containing regulatory region of in
      Deletions and Duplications ( 12 )
      Summary of Phenotypes
      Orthologs
      Human Orthologs (via DIOPT v7.1)
      Homo sapiens (Human) (1)
      Species\Gene Symbol
      Score
      Best Score
      Best Reverse Score
      Alignment
      Complementation?
      Transgene?
      13 of 15
      Yes
      Yes
      Model Organism Orthologs (via DIOPT v7.1)
      Mus musculus (laboratory mouse) (1)
      Species\Gene Symbol
      Score
      Best Score
      Best Reverse Score
      Alignment
      Complementation?
      Transgene?
      12 of 15
      Yes
      Yes
      Rattus norvegicus (Norway rat) (1)
      5 of 13
      Yes
      Yes
      Xenopus tropicalis (Western clawed frog) (1)
      11 of 12
      Yes
      Yes
      Danio rerio (Zebrafish) (1)
      11 of 15
      Yes
      Yes
      Caenorhabditis elegans (Nematode, roundworm) (0)
      No orthologs reported.
      Arabidopsis thaliana (thale-cress) (0)
      No orthologs reported.
      Saccharomyces cerevisiae (Brewer's yeast) (0)
      No orthologs reported.
      Schizosaccharomyces pombe (Fission yeast) (0)
      No orthologs reported.
      Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG091902E6 )
      Organism
      Common Name
      Gene
      AAA Syntenic Ortholog
      Multiple Dmel Genes in this Orthologous Group
      Drosophila melanogaster
      fruit fly
      Drosophila suzukii
      Spotted wing Drosophila
      Drosophila simulans
      Drosophila sechellia
      Drosophila erecta
      Drosophila yakuba
      Drosophila ananassae
      Drosophila pseudoobscura pseudoobscura
      Drosophila persimilis
      Drosophila willistoni
      Drosophila virilis
      Drosophila mojavensis
      Drosophila grimshawi
      Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091502HT )
      Organism
      Common Name
      Gene
      Multiple Dmel Genes in this Orthologous Group
      Musca domestica
      House fly
      Glossina morsitans
      Tsetse fly
      Lucilia cuprina
      Australian sheep blowfly
      Mayetiola destructor
      Hessian fly
      Aedes aegypti
      Yellow fever mosquito
      Anopheles darlingi
      American malaria mosquito
      Anopheles gambiae
      Malaria mosquito
      Culex quinquefasciatus
      Southern house mosquito
      Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W030R )
      Organism
      Common Name
      Gene
      Multiple Dmel Genes in this Orthologous Group
      Bombyx mori
      Silkmoth
      Danaus plexippus
      Monarch butterfly
      Heliconius melpomene
      Postman butterfly
      Apis florea
      Little honeybee
      Apis mellifera
      Western honey bee
      Bombus impatiens
      Common eastern bumble bee
      Bombus terrestris
      Buff-tailed bumblebee
      Linepithema humile
      Argentine ant
      Megachile rotundata
      Alfalfa leafcutting bee
      Nasonia vitripennis
      Parasitic wasp
      Dendroctonus ponderosae
      Mountain pine beetle
      Tribolium castaneum
      Red flour beetle
      Pediculus humanus
      Human body louse
      Rhodnius prolixus
      Kissing bug
      Acyrthosiphon pisum
      Pea aphid
      Zootermopsis nevadensis
      Nevada dampwood termite
      Zootermopsis nevadensis
      Nevada dampwood termite
      Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X02XO )
      Organism
      Common Name
      Gene
      Multiple Dmel Genes in this Orthologous Group
      Strigamia maritima
      European centipede
      Strigamia maritima
      European centipede
      Strigamia maritima
      European centipede
      Ixodes scapularis
      Black-legged tick
      Stegodyphus mimosarum
      African social velvet spider
      Tetranychus urticae
      Two-spotted spider mite
      Daphnia pulex
      Water flea
      Daphnia pulex
      Water flea
      Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G01NC )
      Organism
      Common Name
      Gene
      Multiple Dmel Genes in this Orthologous Group
      Strongylocentrotus purpuratus
      Purple sea urchin
      Strongylocentrotus purpuratus
      Purple sea urchin
      Ciona intestinalis
      Vase tunicate
      Gallus gallus
      Domestic chicken
      Human Disease Model Data
      FlyBase Human Disease Model Reports
        Alleles Reported to Model Human Disease (Disease Ontology)
        Download
        Models ( 0 )
        Allele
        Disease
        Evidence
        References
        Interactions ( 0 )
        Allele
        Disease
        Interaction
        References
        Comments ( 0 )
         
        Human Orthologs (via DIOPT v7.1)
        Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
        Functional Complementation Data
        Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
        Interactions
        Summary of Physical Interactions
        esyN Network Diagram
        Show neighbor-neighbor interactions:
        Select Layout:
        Legend:
        Protein
        RNA
        Selected Interactor(s)
        Interactions Browser

        Please look at the Interaction Group reports for full details of the physical interactions
        protein-protein
        Interacting group
        Assay
        References
        Summary of Genetic Interactions
        esyN Network Diagram
        esyN Network Key:
        Suppression
        Enhancement

        Please look at the allele data for full details of the genetic interactions
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        External Data
        Subunit Structure (UniProtKB)
        Interacts with mwh.
        (UniProt, Q9VPH0 )
        Linkouts
        BioGRID - A database of protein and genetic interactions.
        DroID - A comprehensive database of gene and protein interactions.
        InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
        Pathways
        Gene Group - Pathway Membership (FlyBase)
        External Data
        Linkouts
        Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
        Genomic Location and Detailed Mapping Data
        Chromosome (arm)
        3L
        Recombination map
        3-47
        Cytogenetic map
        Sequence location
        3L:20,367,198..20,370,259 [+]
        FlyBase Computed Cytological Location
        Cytogenetic map
        Evidence for location
        77B6-77B6
        Limits computationally determined from genome sequence between P{PZ}eRF100103 and P{EP}trblEP3519
        Experimentally Determined Cytological Location
        Cytogenetic map
        Notes
        References
        77B3-77B3
        Experimentally Determined Recombination Data
        Left of (cM)
        Right of (cM)
        Notes
        Stocks and Reagents
        Stocks (73)
        Genomic Clones (12)
         

        Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

        cDNA Clones (2)
         

        Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

        cDNA clones, fully sequences
        BDGP DGC clones
        Other clones
          Drosophila Genomics Resource Center cDNA clones

          For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

          cDNA Clones, End Sequenced (ESTs)
          BDGP DGC clones
          Other clones
            RNAi and Array Information
            Linkouts
            DRSC - Results frm RNAi screens
            GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
            Antibody Information
            Laboratory Generated Antibodies
             
            Commercially Available Antibodies
             
            Developmental Studies Hybridoma Bank - Monoclonal antibodies for use in research
            Other Information
            Relationship to Other Genes
            Source for database identify of
            Source for database merge of
            Additional comments
            Other Comments
            in is not required for the domineering nonautonomy caused by fz clones. in and fy are needed in cells receiving and responding to a fz dependent intercellular signal. in and fy are needed for cells to respond to pk and stan. Genetic analysis is consistent with fz-like class of genes fz, pk, Vang, stan and dsh acting as positive regulators of in and fy.
            Mutations in fz-like class of genes fz, pk, Vang, stan and dsh act as strong enhancers of weak in or fy phenotypes. Vinblastine enhances the multiple wing hair phenotype of in and fy mutants indicating that the microtubule cytoskeleton has a function independent of the fz pathway. In the wing as in the abdomen in and fy are epistatic to fz as double mutants resemble in and fy and not fz. In the eye fz is epistatic to in and fy. In terms of the wing eversion phenotype of fz, in and fy appear to be epistatic to fz.
            Genetic mosaics demonstrate in is required in all regions of the wing and the gene product functions in a cell autonomous fashion.
            The in mutant phenotype can be phenocopied by cold treatment or vinblastine treatment in an in vitro system.
            Temperature shift experiments suggest that in functions prior to the actual morphogenesis of the prehair. Mutational analysis reveals in is not an essential gene.
            Comparisons between fz and in alleles reveals that at equivalent fractions of the wing showing abnormal polarity, in mutant wings have many fold more multiple hair cells than fz mutant wings.
            Mutant individuals display hairs and bristles directed toward the midline.
            Origin and Etymology
            Discoverer
            Bridges, 20th Nov. 1926.
            Etymology
            Identification
            External Crossreferences and Linkouts ( 31 )
            Crossreferences
            NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
            GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
            GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
            RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
            UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
            Other crossreferences
            BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
            InterPro - A database of protein families, domains and functional sites
            Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
            Linkouts
            BioGRID - A database of protein and genetic interactions.
            Drosophila Genomics Resource Center - Drosophila Genomics Resource Center cDNA clones
            DroID - A comprehensive database of gene and protein interactions.
            DRSC - Results frm RNAi screens
            Developmental Studies Hybridoma Bank - Monoclonal antibodies for use in research
            Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
            FLIGHT - Cell culture data for RNAi and other high-throughput technologies
            FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
            Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
            Flygut - An atlas of the Drosophila adult midgut
            FlyMine - An integrated database for Drosophila genomics
            Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
            GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
            iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
            InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
            KEGG Genes - Molecular building blocks of life in the genomic space.
            modMine - A data warehouse for the modENCODE project
            Synonyms and Secondary IDs (7)
            Datasets (0)
            Study focus (0)
            Experimental Role
            Project
            Project Type
            Title
            References (114)