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Gene Dmel\ix

General Information
Symbol	Dmel\ix	Species	D. melanogaster
Name	intersex	Annotation symbol	CG13201
Feature type	protein_coding_gene	FlyBase ID	FBgn0001276
Gene Model Status	Current	Stock availability	6 publicly available
Genomic Location
Chromosome (arm)	2R	Recombination map	2-60.5
Cytogenetic map	47F5-47F5	Sequence location	2R:7,246,942..7,247,668 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene intersex is referred to in FlyBase by the symbol Dmel\ix (CG13201, FBgn0001276). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: transcription factor binding; RNA polymerase II transcription cofactor activity. There is experimental evidence that it is involved in the biological process: regulation of transcription from RNA polymerase II promoter; sex determination. 19 alleles are reported. The phenotypes of these alleles are annotated with: female genitalia; sternite; male genitalia; gonopod long bristle; sex comb; female genital disc; tergite; female terminalia; anal plate; spermatozoon. It has one annotated transcript and one annotated polypeptide. Protein features are: Mediator complex, subunit Med29, metazoa. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-06 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Little or no expression detected in any larval or adult organs/tissues. Comments on Affy2 ProbeSet: ProbeSet 1639714_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of ix. Gene sequence location is 2R:7246942..7247668.
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	ix: intersex Females changed into sterile intersexes with a set of reduced male and a set of irregular female external genitalia. Gonads also mixed. They have no sex combs; pigmentaion of abdomen intermediate between male and female. A large mass of chitinized tissue protrudes from vaginal opening. Homozygous males look normal but behave like intersexes (McRobert and Tompkins, 1985). They court young males as much as normal males do, and occasionally court females; however, they are attractive to normal mature males. Heterozygous ix/+ males court females and young males, but seldom court mature males. The ix locus acts with the dsx female function to allow somatic differentiation in females (Nagoshi, McKeown, Burtis, Belote, and Baker, 1988, Cell 53: 229-36). ix2 Females homozygous for ix2 have male-like pigmentation of posterior tergites, rudimentary ovaries, and are sterile. Expression extreme and viability reduced at 27; at 17, expression less extreme and viability greater. Homozygous males appear normal but have nonmotile sperm. RK2. ix3
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2011_10
FB2012_01	Sequence features BKN20552 HFA06325
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 47F5-47F5   Limits computationally determined from genome sequence between P{lacW}Fppsk06103&P{lacW}Fppsk03514 and P{lacW}Tapδk17005&P{lacW}k05103
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location	2-60.5
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\ix for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) ix-RA FBtr0088154  NM_136833   727   188
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein ix-PA   FBpp0087251   21.1   188   6.95     NP_610677   AAF58653
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Mediator complex, subunit Med29, metazoa (IPR021018)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE013599 AAF58653   AF491289 AAN37397   BH759492   BP555661   BT028773   CO179100   CO262571   CO263049   CO288781   CO290275   CO291475   CO314979   CO315001   CO323220   EC058822   EC080135   EC088404   EC207635   EC218678   EC229172   EC230301   EC230934   EC230935   EC237524   EC246589   EC259396
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q7KBL8
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0001276    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0001276 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-06 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0001276 expression level Developmental Stage   Expression Level embryo 00-02hr    3641 embryo 02-04hr    4215 embryo 04-06hr    3050 embryo 06-08hr    2820 embryo 08-10hr    3010 embryo 10-12hr    2791 embryo 12-14hr    2150 embryo 14-16hr    1952 embryo 16-18hr    1316 embryo 18-20hr    946 embryo 20-22hr    1970 embryo 22-24hr    1654 larva L1    608 larva L2    608 larva L3 12hr old    403 larva L3 puffstage 1-2    726 larva L3 puffstage 3-6    917 larva L3 puffstage 7-9    1169 white prepupae new    1136 white prepupae 12hr    1083 white prepupae 24hr    1306 pupae 2d postWPP    1208 pupae 3d postWPP    514 pupae 4d postWPP    429 adult male 01day    419 adult male 05day    427 adult male 30day    545 adult female 01day    936 adult female 05day    1357 adult female 30day    1547 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (3641) embryo 02-04hr  (4215) embryo 04-06hr  (3050) embryo 06-08hr  (2820) embryo 08-10hr  (3010) embryo 10-12hr  (2791) embryo 12-14hr  (2150) embryo 14-16hr  (1952) embryo 16-18hr  (1316) embryo 18-20hr  (946) embryo 20-22hr  (1970) embryo 22-24hr  (1654) larva L1  (608) larva L2  (608) larva L3 12hr old  (403) larva L3 puffstage 1-2  (726) larva L3 puffstage 3-6  (917) larva L3 puffstage 7-9  (1169) white prepupae new  (1136) white prepupae 12hr  (1083) white prepupae 24hr  (1306) pupae 2d postWPP  (1208) pupae 3d postWPP  (514) pupae 4d postWPP  (429) adult male 01day  (419) adult male 05day  (427) adult male 30day  (545) adult female 01day  (936) adult female 05day  (1357) adult female 30day  (1547) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (3641) embryo 02-04hr  (4215) embryo 04-06hr  (3050) embryo 06-08hr  (2820) embryo 08-10hr  (3010) embryo 10-12hr  (2791) embryo 12-14hr  (2150) embryo 14-16hr  (1952) embryo 16-18hr    1316 embryo 18-20hr    946 embryo 20-22hr  (1970) embryo 22-24hr  (1654) larva L1    608 larva L2    608 larva L3 12hr old    403 larva L3 puffstage 1-2    726 larva L3 puffstage 3-6    917 larva L3 puffstage 7-9    1169 white prepupae new    1136 white prepupae 12hr    1083 white prepupae 24hr    1306 pupae 2d postWPP    1208 pupae 3d postWPP    514 pupae 4d postWPP    429 adult male 01day    419 adult male 05day    427 adult male 30day    545 adult female 01day    936 adult female 05day    1357 adult female 30day  (1547) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    3641 embryo 02-04hr    4215 embryo 04-06hr    3050 embryo 06-08hr    2820 embryo 08-10hr    3010 embryo 10-12hr    2791 embryo 12-14hr    2150 embryo 14-16hr    1952 embryo 16-18hr    1316 embryo 18-20hr    946 embryo 20-22hr    1970 embryo 22-24hr    1654 larva L1    608 larva L2    608 larva L3 12hr old    403 larva L3 puffstage 1-2    726 larva L3 puffstage 3-6    917 larva L3 puffstage 7-9    1169 white prepupae new    1136 white prepupae 12hr    1083 white prepupae 24hr    1306 pupae 2d postWPP    1208 pupae 3d postWPP    514 pupae 4d postWPP    429 adult male 01day    419 adult male 05day    427 adult male 30day    545 adult female 01day    936 adult female 05day    1357 adult female 30day    1547 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    3641 embryo 02-04hr    4215 embryo 04-06hr    3050 embryo 06-08hr    2820 embryo 08-10hr    3010 embryo 10-12hr    2791 embryo 12-14hr    2150 embryo 14-16hr    1952 embryo 16-18hr    1316 embryo 18-20hr    946 embryo 20-22hr    1970 embryo 22-24hr    1654 larva L1    608 larva L2    608 larva L3 12hr old    403 larva L3 puffstage 1-2    726 larva L3 puffstage 3-6    917 larva L3 puffstage 7-9    1169 white prepupae new    1136 white prepupae 12hr    1083 white prepupae 24hr    1306 pupae 2d postWPP    1208 pupae 3d postWPP    514 pupae 4d postWPP    429 adult male 01day    419 adult male 05day    427 adult male 30day    545 adult female 01day    936 adult female 05day    1357 adult female 30day    1547 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0001276 expression level Developmental Stage   Expression Level embryo 00-02hr    3641 embryo 02-04hr    4215 embryo 04-06hr    3050 embryo 06-08hr    2820 embryo 08-10hr    3010 embryo 10-12hr    2791 embryo 12-14hr    2150 embryo 14-16hr    1952 embryo 16-18hr    1316 embryo 18-20hr    946 embryo 20-22hr    1970 embryo 22-24hr    1654 larva L1    608 larva L2    608 larva L3 12hr old    403 larva L3 puffstage 1-2    726 larva L3 puffstage 3-6    917 larva L3 puffstage 7-9    1169 white prepupae new    1136 white prepupae 12hr    1083 white prepupae 24hr    1306 pupae 2d postWPP    1208 pupae 3d postWPP    514 pupae 4d postWPP    429 adult male 01day    419 adult male 05day    427 adult male 30day    545 adult female 01day    936 adult female 05day    1357 adult female 30day    1547 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (3641) embryo 02-04hr  (4215) embryo 04-06hr  (3050) embryo 06-08hr  (2820) embryo 08-10hr  (3010) embryo 10-12hr  (2791) embryo 12-14hr  (2150) embryo 14-16hr  (1952) embryo 16-18hr  (1316) embryo 18-20hr  (946) embryo 20-22hr  (1970) embryo 22-24hr  (1654) larva L1  (608) larva L2  (608) larva L3 12hr old  (403) larva L3 puffstage 1-2  (726) larva L3 puffstage 3-6  (917) larva L3 puffstage 7-9  (1169) white prepupae new  (1136) white prepupae 12hr  (1083) white prepupae 24hr  (1306) pupae 2d postWPP  (1208) pupae 3d postWPP  (514) pupae 4d postWPP  (429) adult male 01day  (419) adult male 05day  (427) adult male 30day  (545) adult female 01day  (936) adult female 05day  (1357) adult female 30day  (1547) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (3641) embryo 02-04hr  (4215) embryo 04-06hr  (3050) embryo 06-08hr  (2820) embryo 08-10hr  (3010) embryo 10-12hr  (2791) embryo 12-14hr  2150 embryo 14-16hr  1952 embryo 16-18hr    1316 embryo 18-20hr    946 embryo 20-22hr  1970 embryo 22-24hr  1654 larva L1    608 larva L2    608 larva L3 12hr old    403 larva L3 puffstage 1-2    726 larva L3 puffstage 3-6    917 larva L3 puffstage 7-9    1169 white prepupae new    1136 white prepupae 12hr    1083 white prepupae 24hr    1306 pupae 2d postWPP    1208 pupae 3d postWPP    514 pupae 4d postWPP    429 adult male 01day    419 adult male 05day    427 adult male 30day    545 adult female 01day    936 adult female 05day    1357 adult female 30day  1547 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    3641 embryo 02-04hr    4215 embryo 04-06hr    3050 embryo 06-08hr    2820 embryo 08-10hr    3010 embryo 10-12hr    2791 embryo 12-14hr    2150 embryo 14-16hr    1952 embryo 16-18hr    1316 embryo 18-20hr    946 embryo 20-22hr    1970 embryo 22-24hr    1654 larva L1    608 larva L2    608 larva L3 12hr old    403 larva L3 puffstage 1-2    726 larva L3 puffstage 3-6    917 larva L3 puffstage 7-9    1169 white prepupae new    1136 white prepupae 12hr    1083 white prepupae 24hr    1306 pupae 2d postWPP    1208 pupae 3d postWPP    514 pupae 4d postWPP    429 adult male 01day    419 adult male 05day    427 adult male 30day    545 adult female 01day    936 adult female 05day    1357 adult female 30day    1547 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    3641 embryo 02-04hr    4215 embryo 04-06hr    3050 embryo 06-08hr    2820 embryo 08-10hr    3010 embryo 10-12hr    2791 embryo 12-14hr    2150 embryo 14-16hr    1952 embryo 16-18hr    1316 embryo 18-20hr    946 embryo 20-22hr    1970 embryo 22-24hr    1654 larva L1    608 larva L2    608 larva L3 12hr old    403 larva L3 puffstage 1-2    726 larva L3 puffstage 3-6    917 larva L3 puffstage 7-9    1169 white prepupae new    1136 white prepupae 12hr    1083 white prepupae 24hr    1306 pupae 2d postWPP    1208 pupae 3d postWPP    514 pupae 4d postWPP    429 adult male 01day    419 adult male 05day    427 adult male 30day    545 adult female 01day    936 adult female 05day    1357 adult female 30day    1547 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0001276    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0001276 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Little or no expression detected in any larval or adult organs/tissues. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0001276 expression level Tissue   Expression Level Larval Central Nervous System    96.725 Larval Midgut    40.7 Larval Hindgut    49.3 Larval Malpighian Tubules    52.3 Larval Fat Body no informative data Larval Salivary Gland    58.3 Larval Trachea    53.7 Larval Carcass    38.3 Adult Head    44.6 Adult Eye    51.55 Adult Brain    73.1 Adult Thoracic-Abdominal Ganglion    59.3 Adult Crop    40.7 Adult Midgut    39.3 Adult Hindgut    38.5 Adult Malpighian Tubules    36.1 Adult Fat Body    37.9 Adult Salivary Gland no informative data Adult Heart    35.9 Adult VirginFemale Spermatheca    51.4 Adult InseminatedFemale Spermatheca    51.3 Adult Ovary    98.7 Adult Testis    7.1 Adult Male Accessory Gland    52.8 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    96.725 Larval Midgut    40.7 Larval Hindgut    49.3 Larval Malpighian Tubules    52.3 Larval Fat Body no informative data Larval Salivary Gland    58.3 Larval Trachea    53.7 Larval Carcass    38.3 Adult Head    44.6 Adult Eye    51.55 Adult Brain    73.1 Adult Thoracic-Abdominal Ganglion    59.3 Adult Crop    40.7 Adult Midgut    39.3 Adult Hindgut    38.5 Adult Malpighian Tubules    36.1 Adult Fat Body    37.9 Adult Salivary Gland no informative data Adult Heart    35.9 Adult VirginFemale Spermatheca    51.4 Adult InseminatedFemale Spermatheca    51.3 Adult Ovary    98.7 Adult Testis    7.1 Adult Male Accessory Gland    52.8 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    96.725 Larval Midgut    40.7 Larval Hindgut    49.3 Larval Malpighian Tubules    52.3 Larval Fat Body no informative data Larval Salivary Gland    58.3 Larval Trachea    53.7 Larval Carcass    38.3 Adult Head    44.6 Adult Eye    51.55 Adult Brain    73.1 Adult Thoracic-Abdominal Ganglion    59.3 Adult Crop    40.7 Adult Midgut    39.3 Adult Hindgut    38.5 Adult Malpighian Tubules    36.1 Adult Fat Body    37.9 Adult Salivary Gland no informative data Adult Heart    35.9 Adult VirginFemale Spermatheca    51.4 Adult InseminatedFemale Spermatheca    51.3 Adult Ovary    98.7 Adult Testis    7.1 Adult Male Accessory Gland    52.8 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    96.725 Larval Midgut    40.7 Larval Hindgut    49.3 Larval Malpighian Tubules    52.3 Larval Fat Body no informative data Larval Salivary Gland    58.3 Larval Trachea    53.7 Larval Carcass    38.3 Adult Head    44.6 Adult Eye    51.55 Adult Brain    73.1 Adult Thoracic-Abdominal Ganglion    59.3 Adult Crop    40.7 Adult Midgut    39.3 Adult Hindgut    38.5 Adult Malpighian Tubules    36.1 Adult Fat Body    37.9 Adult Salivary Gland no informative data Adult Heart    35.9 Adult VirginFemale Spermatheca    51.4 Adult InseminatedFemale Spermatheca    51.3 Adult Ovary    98.7 Adult Testis    7.1 Adult Male Accessory Gland    52.8 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0001276 expression level Tissue   Expression Level Larval Central Nervous System    96.725 Larval Midgut    40.7 Larval Hindgut    49.3 Larval Malpighian Tubules    52.3 Larval Fat Body no informative data Larval Salivary Gland    58.3 Larval Trachea    53.7 Larval Carcass    38.3 Adult Head    44.6 Adult Eye    51.55 Adult Brain    73.1 Adult Thoracic-Abdominal Ganglion    59.3 Adult Crop    40.7 Adult Midgut    39.3 Adult Hindgut    38.5 Adult Malpighian Tubules    36.1 Adult Fat Body    37.9 Adult Salivary Gland no informative data Adult Heart    35.9 Adult VirginFemale Spermatheca    51.4 Adult InseminatedFemale Spermatheca    51.3 Adult Ovary    98.7 Adult Testis    7.1 Adult Male Accessory Gland    52.8 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    96.725 Larval Midgut    40.7 Larval Hindgut    49.3 Larval Malpighian Tubules    52.3 Larval Fat Body no informative data Larval Salivary Gland    58.3 Larval Trachea    53.7 Larval Carcass    38.3 Adult Head    44.6 Adult Eye    51.55 Adult Brain    73.1 Adult Thoracic-Abdominal Ganglion    59.3 Adult Crop    40.7 Adult Midgut    39.3 Adult Hindgut    38.5 Adult Malpighian Tubules    36.1 Adult Fat Body    37.9 Adult Salivary Gland no informative data Adult Heart    35.9 Adult VirginFemale Spermatheca    51.4 Adult InseminatedFemale Spermatheca    51.3 Adult Ovary    98.7 Adult Testis    7.1 Adult Male Accessory Gland    52.8 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    96.725 Larval Midgut    40.7 Larval Hindgut    49.3 Larval Malpighian Tubules    52.3 Larval Fat Body no informative data Larval Salivary Gland    58.3 Larval Trachea    53.7 Larval Carcass    38.3 Adult Head    44.6 Adult Eye    51.55 Adult Brain    73.1 Adult Thoracic-Abdominal Ganglion    59.3 Adult Crop    40.7 Adult Midgut    39.3 Adult Hindgut    38.5 Adult Malpighian Tubules    36.1 Adult Fat Body    37.9 Adult Salivary Gland no informative data Adult Heart    35.9 Adult VirginFemale Spermatheca    51.4 Adult InseminatedFemale Spermatheca    51.3 Adult Ovary    98.7 Adult Testis    7.1 Adult Male Accessory Gland    52.8 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    96.725 Larval Midgut    40.7 Larval Hindgut    49.3 Larval Malpighian Tubules    52.3 Larval Fat Body no informative data Larval Salivary Gland    58.3 Larval Trachea    53.7 Larval Carcass    38.3 Adult Head    44.6 Adult Eye    51.55 Adult Brain    73.1 Adult Thoracic-Abdominal Ganglion    59.3 Adult Crop    40.7 Adult Midgut    39.3 Adult Hindgut    38.5 Adult Malpighian Tubules    36.1 Adult Fat Body    37.9 Adult Salivary Gland no informative data Adult Heart    35.9 Adult VirginFemale Spermatheca    51.4 Adult InseminatedFemale Spermatheca    51.3 Adult Ovary    98.7 Adult Testis    7.1 Adult Male Accessory Gland    52.8 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body no informative data Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland no informative data Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass no informative data FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    44.6   NA  Eye    51.55   NA  Brain    73.1   96.725  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    59.3   NA  Crop    40.7   40.7  Midgut    39.3   49.3  Hindgut    38.5   52.3  Malpighian Tubules    36.1   no informative data  Fat Body    37.9   58.3  Salivary Gland    no informative data   NA  Heart    35.9   53.7  Trachea    NA   NA  VirginFemale Spermatheca    51.4   NA  InseminatedFemale Spermatheca    51.3   NA  Ovary    98.7   NA  Testis    7.1   NA  Male Accessory Gland    52.8   38.3  Carcass    no informative data modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_17
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0001276 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG13201-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | recessive ixPH viable, with Scer\GAL4pnr-MD237 ixGD1468 Sterility Allele female sterile ix3 male semi-sterile | heat sensitive ix5 semi-sterile | recessive | heat sensitive ix4 ix87i3 Other Phenotypes Allele courtship behavior defective ix5 sex-determination defective ix1 ix2 sex-determination defective | female | heat sensitive ix5 sex-determination defective | male | heat sensitive ix5 sex-determination defective | recessive ix1 ix2 visible ix1 ix2 ix3 visible | heat sensitive ix4 Phenotype manifest in Allele abdominal tergite 6 | female (with Df(2R)en-B) ix3 anal plate ix1 ix2 ix3 ix5 anal plate | ectopic ix1 ix2 anal plate | female (with Df(2R)en-B) ix3 female genital disc ix5 female genitalia ix1 ix2 ix3 female terminalia ix1 ix2 ix5 genital arch | ectopic ix5 gonopod long bristle (with Df(2R)en-B) ix3 lateral plate | ectopic ix5 leg | male ix5 male genital disc primordium | heat sensitive ix5 male genitalia ix1 ix2 ix3 male terminalia | ectopic ix1 ix2 ix5 penis | ectopic ix5 prothoracic tarsal segment 1 transverse row | female (with Df(2R)en-B) ix3 sex comb ix1 ix5 spermatozoon ix5 sternite ix1 ix2 ix3 tergite ix1 ix2 ix3 testis | heat sensitive ix5
Classical Alleles ( 9 )
For All Classical Alleles Show
Allele of ix Class Mutagen Stocks Known lesion ix2 loss of function allele U.V. 2 Yes ix1 loss of function allele spontaneous 1 Yes ixKG04582 P-element activity 1 -- ix3 loss of function allele ethyl methanesulfonate 0 -- ix4 X ray 0 -- ix5 loss of function allele spontaneous 0 -- ix87i3 PM hybrid dysgenesis 0 Yes ixPH X ray 0 -- ixunspecified 0 --
Alleles Carried on Transgenic Constructs ( 10 )
For All Alleles Carried on Transgenic Constructs Show
Allele of ix Class Mutagen Stocks Known lesion ixGD1468 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes ixKK100456 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes ix+t1GB in vitro construct - genomic fragment 0 Yes ix+t2GB in vitro construct - genomic fragment 0 Yes ix+t3GBRΔ in vitro construct - genomic fragment in vitro construct - deletion 0 Yes ix+t3GBsan.mut in vitro construct - genomic fragment in vitro construct - deletion in vitro construct - site directed mutagenesis in vitro construct - amino acid replacement 0 Yes ixhs.PG in vitro construct - regulatory fusion 0 Yes ixMtnA.T:BPV-1\AU1 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes ixScer\UAS.P\T.cSa in vitro construct - regulatory fusion 0 Yes ixt3GBix.mut in vitro construct - genomic fragment in vitro construct - deletion in vitro construct - site directed mutagenesis in vitro construct - amino acid replacement 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2R)17 (Garrett-Engele et al., 2002) Df(2R)BSC703 (Christensen et al., 2008.12.28) Df(2R)en-A (Chase and Baker, 1995) Df(2R)en-B (Garrett-Engele et al., 2002, Acharyya and Chatterjee, 2002, Siegal and Baker, 2005, Chase and Baker, 1995) Df(2R)Exel6060 (Ryder, 2004.4.26) Df(2R)ix87i3 (Garrett-Engele et al., 2002, Chase and Baker, 1995) Df(2R)wxba9x1 (Chase and Baker, 1995) Df(2R)wxba9x3 (Chase and Baker, 1995) Df(2R)wxba9x4 (Chase and Baker, 1995)
Not disrupted in	Df(2R)27 (Garrett-Engele et al., 2002) Df(2R)en-SFX31 (Chase and Baker, 1995)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD1468} NA P{KK100456} NA P{UASp-ix.S} NA heat-shock construct P{hs-ix.G} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions P{SUPor-P}ixKG04582 NA
Gene Ontology: Function, Process & Cellular Component ( 8 unique terms )
Terms Based on Experimental Evidence ( 5 terms )
Molecular Function
CV term Evidence References
RNA polymerase II transcription cofactor activity inferred from direct assay (assigned by UniProtKB) (Garrett-Engele et al., 2002) transcription factor binding inferred from physical interaction with dsx (Yang et al., 2008)
Biological Process
CV term Evidence References
regulation of transcription from RNA polymerase II promoter inferred from direct assay (assigned by UniProtKB) (Garrett-Engele et al., 2002) sex determination inferred from mutant phenotype (Acharyya and Chatterjee, 2002)
Cellular Component
CV term Evidence References
mediator complex inferred from direct assay (assigned by UniProtKB) (Garrett-Engele et al., 2002)
Terms Based on Predictions or Assertions ( 4 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
female somatic sex determination traceable author statement (Zarkower, 2001) sex differentiation traceable author statement (Billeter et al., 2002) somatic sex determination non-traceable author statement (Schutt and Nothiger, 2000)
Cellular Component
CV term Evidence References
mediator complex inferred from sequence or structural similarity (Bourbon et al., 2004)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions ix allele Gene References ix3 dsx (Garrett-Engele et al., 2002) ixunspecified dsx (Nothiger et al., 1987)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 69950 DroID - A comprehensive database of gene and protein interactions. • FBgn0001276 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 45881
Orthologs
Genome-wide drosophilid orthologs	Dana\GF12399 Dere\GG22655 Dgri\GH20513 Dmoj\GI21058 Dpse\GA12116 Dsec\GM20436 Dsim\GD15252 Dvir\GJ21983 Dwil\GK24513 Dyak\GE13530
Curated drosophilid orthologs	Dpse\GA12116   Dsim\ix (Sturtevant, 1929)
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AAEL010217-PB Aedes aegypti (mosquito) AGAP001237-PA Anopheles gambiae (mosquito) XP_395989.1 Apis mellifera (honey bee) XP_001944206.1 Acyrthosiphon pisum (pea aphid) 262443 Branchiostoma floridae (lancelet) BGIBMGA010131-PA Bombyx mori (silk worm) ENSBTAP00000027226 Bos taurus (bovine) ENSCAFP00000008268 Canis familiaris (dog) CPIJ008343 Culex pipens (mosquito) ENSCSAVP00000008300 Ciona savignyi (sedentary tunicate) 50878 Daphnia pulex (daphnia) ENSDARP00000031379 Danio rerio (zebra fish) ENSECAP00000015836 Equus caballus (horse) ENSGACP00000012181 Gymnopilus aculeatus (mushroom) ENSP00000314343 Homo sapiens (human) 159839 Lottia gigantea (owl limpet) ENSMODP00000016871 Monodelphis domestica (gray opossum) ENSMMUP00000007339 Macaca mulatta (rhesus monkey) ENSMUSP00000003536 Mus musculus (house mouse) 247736 Nematostella vectensis (starlet anemone) XP_001601927.1 Nasonia vitripennis (wasp) ENSORLP00000009250 Oryzias latipes (japanese killifish) PHUM008383-PA Pediculus humanus (human body louse) ENSPPYP00000011155 Pongo pygmaeus (orangutan) ENSRNOP00000026721 Rattus norvegicus (norway rat) XP_783567.1 Strongylocentrotus purpuratus (purple sea urchin) ENSTNIP00000006061 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000036261 Takifugu rubripes (pufferfish Takifugu) ENSXETP00000057016 Xenopus tropicalis (frog) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV17157 LH24093 FBgn0231196 FBgn0226473 FBgn0209097 FBgn0186920 FBgn0175318 JGI_V11_50878 FBgn0072164 FBgn0143790 FBgn0127976 FBgn0114826 FBgn0089436 CPIJ008343 BGIBMGA010131 ACYPI003233 GB19364 MED29_ANOGA MED29_AEDAE PB22582 PHUM298000 GLEAN_10345
Stocks & Reagents
Stocks Listed in FlyBase ( 6 )
Bloomington	372 pr1 cn1 ix1/SM5 13822 y1 w67c23; P{SUPor-P}ixKG04582 205 ab2 ix2 bw1 sp2/In(2L)Cy, In(2R)Cy, Cy1 dplv1 Bl1 L4 sp2
Kyoto	105786 ab2 ix2 bw1 sp2/In(2L)Cy, In(2R)Cy, Cy1 dplv1 Bl1 L4 sp2
VDRC	v103405 P{KK100456}VIE-260B v2976 w1118; P{GD1468}v2976
Genomic Clones ( 1 )
	BACR29G22 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 22 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	IP01844
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones
Other clones	64167 119030 119469 214315.25 CK01.20D.C11 CK01.20D.E7 CK01.74B.C5 CK01.74C.F10 CK01.87D.D4 CK01.93B.E12 CK01.101D.D7 EK058411 EK069346 EK073538 EK079608 EK085816 EK087207 EK189961 EK238640 EK238719 FGM236E02
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0001276 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 45881
Antibody Information
Other Information
Discoverer
	Morgan, 1943.
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: ix CG13201 (FlyBase, 1996-)
Additional comments	Source for merge of ix CG13201 was sequence comparison (date:021108). (FlyBase, 1996-)
Other Comments
	ix is required for complete differentiation of female but not male specific adult cuticular structures. (Garrett-Engele et al., 2002) her acts together with the last genes in the determination hierarchy, dsx and ix, to control female sexual differentiation. (Li and Baker, 1998) Sex determining genes tra, ix and dsx have no role in regulating the template organisation of the X chromosome(s) for dosage compensation. (Acharyya and Chatterjee, 1997) Levels of haemolymph vitellogenin of ix flies tentatively suggest ix gene function is neither required for vitellogenin synthesis nor for maintaining proper level of dosage compensation of yolk protein genes. In ix mutant males the yolk protein gene expression is further repressed which suggests the gene is inactive at least in the fat body cells of the males. (Chatterjee and Mukherjee, 1996) At the morphological level, a loss of ix+ function results in the simultaneous realization of both male and female developmental pathways in diplo-X individuals. The ix product is required to function with the female-specific product of dsx to implement appropriate female sexual differentiation in diplo-X individuals. ix does not have a role in sexual behaviour in haplo-X individuals. Clonal analysis suggests that ix functions in a cell-autonomous manner in the abdomen and that it is required in the abdominal histoblasts after the resumption of cell division at pupariation for the normal sexual development of the abdominal tergites. (Chase and Baker, 1995) Mutant analysis of ix suggests that ix is active in both males and females for sexual differentiation. (Chatterjee, 1995) ix plays no role in the development of sexually dimorphic skeletal muscles. (Taylor, 1992)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_136833 RefSeq (Proteins) • NP_610677 DDBJ / EMBL / GenBank • AAF58653 AAN37397 AF491289 BH759492 Entrez Gene - A searchable database of RefSeq genes. • 45881 UniProtKB/TrEMBL • Q7KBL8
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Mediator complex, subunit Med29, metazoa (IPR021018)
Linkouts
	BioGRID - A database of protein and genetic interactions • 69950 DroID - A comprehensive database of gene and protein interactions. • FBgn0001276 DRSC - Results from RNAi screens. • FBgn0001276 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0001276 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG13201-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0001276 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 45881 InParanoid A subset of ortholog calls from InParanoid. • AAEL010217-PB Aedes aegypti (mosquito) AGAP001237-PA Anopheles gambiae (mosquito) XP_395989.1 Apis mellifera (honey bee) XP_001944206.1 Acyrthosiphon pisum (pea aphid) 262443 Branchiostoma floridae (lancelet) BGIBMGA010131-PA Bombyx mori (silk worm) ENSBTAP00000027226 Bos taurus (bovine) ENSCAFP00000008268 Canis familiaris (dog) CPIJ008343 Culex pipens (mosquito) ENSCSAVP00000008300 Ciona savignyi (sedentary tunicate) 50878 Daphnia pulex (daphnia) ENSDARP00000031379 Danio rerio (zebra fish) ENSECAP00000015836 Equus caballus (horse) ENSGACP00000012181 Gymnopilus aculeatus (mushroom) ENSP00000314343 Homo sapiens (human) 159839 Lottia gigantea (owl limpet) ENSMODP00000016871 Monodelphis domestica (gray opossum) ENSMMUP00000007339 Macaca mulatta (rhesus monkey) ENSMUSP00000003536 Mus musculus (house mouse) 247736 Nematostella vectensis (starlet anemone) XP_001601927.1 Nasonia vitripennis (wasp) ENSORLP00000009250 Oryzias latipes (japanese killifish) PHUM008383-PA Pediculus humanus (human body louse) ENSPPYP00000011155 Pongo pygmaeus (orangutan) ENSRNOP00000026721 Rattus norvegicus (norway rat) XP_783567.1 Strongylocentrotus purpuratus (purple sea urchin) ENSTNIP00000006061 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000036261 Takifugu rubripes (pufferfish Takifugu) ENSXETP00000057016 Xenopus tropicalis (frog) InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 45881 modMine - Data generated by the modENCODE project. • FBgn0001276 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV17157 LH24093 FBgn0231196 FBgn0226473 FBgn0209097 FBgn0186920 FBgn0175318 JGI_V11_50878 FBgn0072164 FBgn0143790 FBgn0127976 FBgn0114826 FBgn0089436 CPIJ008343 BGIBMGA010131 ACYPI003233 GB19364 MED29_ANOGA MED29_AEDAE PB22582 PHUM298000 GLEAN_10345
Synonyms & Secondary IDs ( 6 )
Reported As
Symbol Synonym	CG13201 (Garrett-Engele et al., 2002, Keleman et al., 2009.8.5) ix (Yang et al., 2008, Christensen et al., 2008.12.28, Sanders and Arbeitman, 2008, Shirangi et al., 2006, Tarone et al., 2005) MED29 (Bourbon et al., 2004)
Name Synonym	Intersex (Bourbon et al., 2004) intersex (Shirangi et al., 2006, Tarone et al., 2005) Mediator complex subunit 29 (Bourbon et al., 2004)
Secondary FlyBase IDs
	FBgn0033625
References ( 70 )
Generate a list of	All references relating to this gene ( 70 )
List References by type	Research paper  ( 30 ) Supplementary material  ( 3 ) Review  ( 21 ) Personal communication to FlyBase  ( 4 ) Abstract  ( 8 ) FlyBase analysis  ( 1 ) Letter  ( 1 ) Computer file  ( 1 ) Book  ( 1 )
Recent research papers (0)
	All research papers listed in FlyBase were published before 2010 Show research papers ...
Recent reviews (0)
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