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Gene Dmel\Jra

General Information
Symbol	Dmel\Jra	Species	D. melanogaster
Name	Jun-related antigen	Annotation symbol	CG2275
Feature type	protein_coding_gene	FlyBase ID	FBgn0001291
Gene Model Status	Current	Stock availability	7 publicly available
Also Known As	Djun, jun, D-jun, AP-1, AP1, c-Jun, dJRA, l(2)IA109, dAP-1
Genomic Location
Chromosome (arm)	2R	Recombination map	2-60
Cytogenetic map	46E4-46E5	Sequence location	2R:5,983,985..5,986,061 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Jun-related antigen is referred to in FlyBase by the symbol Dmel\Jra (CG2275, FBgn0001291). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: protein binding; sequence-specific DNA binding RNA polymerase II transcription factor activity; protein heterodimerization activity. There is experimental evidence that it is involved in the biological process: negative regulation of antimicrobial humoral response; micropyle formation; positive regulation of transcription from RNA polymerase II promoter; JNK cascade; synaptic growth at neuromuscular junction; wound healing; imaginal disc fusion, thorax closure; dorsal appendage formation; phagocytosis, engulfment. 31 alleles are reported. The phenotypes of these alleles are annotated with: external compound sense organ; organ system subdivision; peripheral nervous system; organ system; histaminergic neuron; imaginal precursor; cell part; late extended germ band embryo; rhabdomere; mesectoderm derivative; adult segment. It has 3 annotated transcripts and 3 annotated polypeptides. Protein features are: Basic-leucine zipper domain; Eukaryotic transcription factor, Skn-1-like, DNA-binding; Jun-like transcription factor; Transcription factor Jun. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 18-24 hour embryonic stages, during early pupal stages. Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult tissues/organs expressed at moderate levels. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male reproductive system, larval/adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1635224_s_at completely aligns to an exonic region common to each of the 2 FlyBase-annotated transcript isoforms of Jra. Gene sequence location is 2R:5983985..5986061.
User Contributed Data
	Beta version, feedback welcome
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03	Controlled Vocabulary Terms A1-7 ventral motor neuron A1-7 VL3/4 motor neuron
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 46E4-46E5   Limits computationally determined from genome sequence between P{lacW}Adamk13906&P{EP}Pka-R2EP2162 and P{PZ}14-3-3ζ07103
Experimentally Determined Cytological Location
Cytogenetic map Notes References 46E-46E   (determined by in situ hybridisation)   (Zhang et al., 1990) 46E-46E   (determined by in situ hybridisation)   (Perkins et al., 1990) 46E-46E   (determined by in situ hybridisation)   (Perkins et al., 1988)
Experimentally Determined Recombination Data
Location	2-60 (Nusslein-Volhard et al., 1984, Tearle and Nusslein-Volhard, 1987)
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Jra for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	gene_with_stop_codon_read_through ; SO:0000697 Stop-codon suppression (UAA) postulated; FBrf0216884 Gene model reviewed during 5.44 Gene model reviewed during 5.49
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Jra-RA FBtr0088411  NM_057238   1525   289 Jra-RB FBtr0088410  NM_165739   1565   289 Jra-RC FBtr0330606  NM_001273915   1525   372
Additional Transcript Data & Comments
Reported size (kB)	1.7 (northern blot) (Perkins et al., 1990, Zhang et al., 1990)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Jra-PA   FBpp0087499   31.0   289   10.06     NP_476586   AAF58845 Jra-PB   FBpp0087498   31.0   289   10.06     NP_724882   AAM71059 Jra-PC   FBpp0303456   39.9   372   4.59     NP_001260844   AGB93377
Additional Polypeptide Data & Comments
Reported size (kDa)	289 (aa); 40 (kD observed) (Perkins et al., 1990) 289 (aa) (Zhang et al., 1990)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Transcription factor Jun (IPR002112) Basic-leucine zipper domain (IPR004827) Jun-like transcription factor (IPR005643) Eukaryotic transcription factor, Skn-1-like, DNA-binding (IPR008917)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA941201   AE013599 AAF58845   AE013599 AAM71059   AC005469   AC005974   AE013599 AGB93377   AI513713   AI517601   AW942721   AY058562 AAL13791   BF495562   BF496150   BF496387   BI170369   BI170596   BI227477   BI229034   BI229036   BI352126   BI619733   BI632402   BI635556   BI637989   BP558817   CO152887   CO155570   CO156502   CO156703   CO178625   CO291527   CO309797   CO325768   CO338774   EC056139   EC201988   EC212330   EC218629   EC223955   EC227748   EC245117   EC262941   EC264017   EC265394   EC267614   EC268045   EV592078   L33044   M36181 AAA28650   X54144 CAA38083   Y12573 CAA73154
UniProtKB/Swiss-Prot	P18289
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References enhancer FBsf0000161134 2R:5,984,058..5,984,063 comment=Description: Jun-related antigen; putative evidence=experimental standard_name=ecdysone site comment=From GB: L33044. Putative ecdysone site at bases 33-38 in L33044. (Wang and Goldstein, 1994) enhancer FBsf0000161136 2R:5,984,049..5,984,054 comment=Description: Jun-related antigen; putative evidence=experimental standard_name=ecdysone site comment=From GenBank:L33044. Ecdysone site at bases 23-28 in L33044. (Wang and Goldstein, 1994) enhancer FBsf0000161137 2R:5,984,140..5,984,145 comment=Description: Jun-related antigen; putative evidence=experimental standard_name=ecdysone site comment=From GenBank:L33044. Ecdysone site at bases 114-119 in L33044. (Wang and Goldstein, 1994) enhancer FBsf0000161138 2R:5,984,338..5,984,344 evidence=experimental standard_name=AP-1 comment=Description: Jun-related antigen; putative comment=From GenBank:L33044. AP-1 binding site at bases 336-342 in L33044. (Wang and Goldstein, 1994) enhancer FBsf0000161139 2R:5,984,361..5,984,368 comment=Description: Jun-related antigen; putative evidence=experimental standard_name=AP-1 comment=From GenBank:L33044. Putative AP-1 binding site at bases 359-366 in L33044. (Wang and Goldstein, 1994) enhancer FBsf0000161140 2R:5,984,414..5,984,421 evidence=experimental standard_name=DTF-1 comment=Description: Jun-related antigen; putative comment=Putative Dtf-1 binding site. Binding site from FBrf0076455. Site is offset by one base from enhancer feature in GB:L33044. (Wang and Goldstein, 1994) protein binding site FBsf0000160589 2R:5,984,413..5,984,435 evidence=experimental (Bergman et al., 2005, Wang and Goldstein, 1994) regulatory region FBsf0000435273 2R:5,984,409..5,984,450 evidence=Reporter construct (in vitro) (Wang and Goldstein, 1994, Halfon, 2012.6.19)
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 4 -- 6   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 7 -- 8   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 9 -- 10   amnioserosa primordium   (Fisher et al., 2012) organism | ubiquitous   (Fisher et al., 2012) embryonic stage 11 -- 12   amnioserosa   (Fisher et al., 2012) organism | ubiquitous   (Fisher et al., 2012) embryonic stage 13 -- 16   organism | ubiquitous   (Fisher et al., 2012) northern blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage   organism | ubiquitous   (Zhang et al., 1990, Perkins et al., 1990) embryonic stage -- adult stage     (Zhang et al., 1990, Perkins et al., 1990) late third instar larval stage -- prepupal stage P4(i)   embryonic/larval salivary gland   (Lehmann et al., 2002)
Additional Descriptive Data
	Jra transcripts are detected at a relatively constant level throughout development on northern blots. In situ hybridization shows that they are expressed uniformly at a low level in all cell types. (Perkins et al., 1990) Jra transcripts are observed throughout development at relatively constant levels on northern blots. By in situ hybridization, they are found in embyros in all tissues. (Zhang et al., 1990) Jra transcript is expressed in salivary glands from 18 hours prior to puparium formation to 12 hours APF. Expression peaks from the middle of the late larval ecdysone pulse though 2 hours APF. (Lehmann et al., 2002)
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
No Assay Recorded Stage Tissue/Position (including subcellular localization) Reference third instar larval stage   photoreceptor cell   (FlyBase, 1992-) ommatidial precursor cluster   (FlyBase, 1992-) immunolocalization Stage Tissue/Position (including subcellular localization) Reference embryonic stage   cephalic furrow   (Kockel et al., 1997) amnioserosa   (Kockel et al., 1997) embryonic stage 11 -- 14   amnioserosa   • nucleus (Reed et al., 2001) embryonic leading edge cell   • nucleus (Reed et al., 2001) embryonic stage 12 -- 17   dorsal ectoderm   (Kockel et al., 1997) embryonic stage 14   amnioserosa   • cytoplasm | punctate (Reed et al., 2001) epidermis | lateral   (Reed et al., 2001) larval stage   motor neuron   (Sanyal et al., 2003) aCC neuron   (Sanyal et al., 2003) RP2 motor neuron   (Sanyal et al., 2003) RP3 motor neuron   (Sanyal et al., 2003) RP5 motor neuron   (Sanyal et al., 2003) third instar larval stage   morphogenetic furrow | posterior to   (FlyBase, 1992-) cone cell   (FlyBase, 1992-) mystery cell   (FlyBase, 1992-)
Additional Descriptive Data
	Jra protein expression is widespread during embryogenesis. One tissue showing elevated Jra expression is the amnioserosa. During and following germ band retraction, the cells of the dorsal epidermis show elevated levels of Jra protein. The highest levels are found in the cells at the leading dorsal edge of the epidermis which are involved in the process of dorsal closure. Expression remains high in these cells throughout the process of dorsal closure. Elevated expression is also observed along the cephalic furrow. (Kockel et al., 1997) Jra protein is expressed posterior to the morphogenetic furrow in all cells entering the ommatidial cluster including photoreceptor cells, cone cells and the mystery cell. Its expression is transient. It is expressed in the cells as they are newly recruited to the ommatidial cluster and for 3-4 columns posteriorly. (FlyBase, 1992-) Jra protein is expressed in the same motor neurons that express the Scer\GAL4[futsch-C380] driver. (Sanyal et al., 2003)
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
cytoplasm inferred from direct assay (Reed et al., 2001) colocalizes_with cytoplasm inferred from direct assay (Jemc et al., 2012) nucleus inferred from direct assay (Reed et al., 2001) colocalizes_with nucleus inferred from direct assay (Jemc et al., 2012) transcription factor complex inferred from physical interaction with kay (Perkins et al., 1990)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult tissues/organs expressed at moderate levels. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male reproductive system, larval/adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    118.225 Larval Midgut    250.8 Larval Hindgut    243.7 Larval Malpighian Tubules    357.6 Larval Fat Body    167.6 Larval Salivary Gland    228.3 Larval Trachea    293.65 Larval Carcass    231.7 Adult Head    160.4 Adult Eye    161.35 Adult Brain    145.1 Adult Thoracic-Abdominal Ganglion    184.4 Adult Crop    339.3 Adult Midgut    230.8 Adult Hindgut    255 Adult Malpighian Tubules    416.6 Adult Fat Body    94 Adult Salivary Gland    173.5 Adult Heart    147.6 Adult VirginFemale Spermatheca    133.8 Adult InseminatedFemale Spermatheca    146.7 Adult Ovary    283.4 Adult Testis    139 Adult Male Accessory Gland    137.9 Adult Carcass    140.6 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    118.225 Larval Midgut    250.8 Larval Hindgut    243.7 Larval Malpighian Tubules    357.6 Larval Fat Body    167.6 Larval Salivary Gland    228.3 Larval Trachea    293.65 Larval Carcass    231.7 Adult Head    160.4 Adult Eye    161.35 Adult Brain    145.1 Adult Thoracic-Abdominal Ganglion    184.4 Adult Crop    339.3 Adult Midgut    230.8 Adult Hindgut    255 Adult Malpighian Tubules    416.6 Adult Fat Body    94 Adult Salivary Gland    173.5 Adult Heart    147.6 Adult VirginFemale Spermatheca    133.8 Adult InseminatedFemale Spermatheca    146.7 Adult Ovary    283.4 Adult Testis    139 Adult Male Accessory Gland    137.9 Adult Carcass    140.6 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    118.225 Larval Midgut    250.8 Larval Hindgut    243.7 Larval Malpighian Tubules    357.6 Larval Fat Body    167.6 Larval Salivary Gland    228.3 Larval Trachea    293.65 Larval Carcass    231.7 Adult Head    160.4 Adult Eye    161.35 Adult Brain    145.1 Adult Thoracic-Abdominal Ganglion    184.4 Adult Crop    339.3 Adult Midgut    230.8 Adult Hindgut    255 Adult Malpighian Tubules    416.6 Adult Fat Body    94 Adult Salivary Gland    173.5 Adult Heart    147.6 Adult VirginFemale Spermatheca    133.8 Adult InseminatedFemale Spermatheca    146.7 Adult Ovary    283.4 Adult Testis    139 Adult Male Accessory Gland    137.9 Adult Carcass    140.6 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    118.225 Larval Midgut    250.8 Larval Hindgut    243.7 Larval Malpighian Tubules    357.6 Larval Fat Body    167.6 Larval Salivary Gland    228.3 Larval Trachea    293.65 Larval Carcass    231.7 Adult Head    160.4 Adult Eye    161.35 Adult Brain    145.1 Adult Thoracic-Abdominal Ganglion    184.4 Adult Crop    339.3 Adult Midgut    230.8 Adult Hindgut    255 Adult Malpighian Tubules    416.6 Adult Fat Body    94 Adult Salivary Gland    173.5 Adult Heart    147.6 Adult VirginFemale Spermatheca    133.8 Adult InseminatedFemale Spermatheca    146.7 Adult Ovary    283.4 Adult Testis    139 Adult Male Accessory Gland    137.9 Adult Carcass    140.6 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    118.225 Larval Midgut    250.8 Larval Hindgut    243.7 Larval Malpighian Tubules    357.6 Larval Fat Body    167.6 Larval Salivary Gland    228.3 Larval Trachea    293.65 Larval Carcass    231.7 Adult Head    160.4 Adult Eye    161.35 Adult Brain    145.1 Adult Thoracic-Abdominal Ganglion    184.4 Adult Crop    339.3 Adult Midgut    230.8 Adult Hindgut    255 Adult Malpighian Tubules    416.6 Adult Fat Body    94 Adult Salivary Gland    173.5 Adult Heart    147.6 Adult VirginFemale Spermatheca    133.8 Adult InseminatedFemale Spermatheca    146.7 Adult Ovary    283.4 Adult Testis    139 Adult Male Accessory Gland    137.9 Adult Carcass    140.6 Expression Level Scale  None   Low   Moderate   High  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    118.225 Larval Midgut    250.8 Larval Hindgut    243.7 Larval Malpighian Tubules    357.6 Larval Fat Body    167.6 Larval Salivary Gland    228.3 Larval Trachea    293.65 Larval Carcass    231.7 Adult Head    160.4 Adult Eye    161.35 Adult Brain    145.1 Adult Thoracic-Abdominal Ganglion    184.4 Adult Crop    339.3 Adult Midgut    230.8 Adult Hindgut    255 Adult Malpighian Tubules    416.6 Adult Fat Body    94 Adult Salivary Gland    173.5 Adult Heart    147.6 Adult VirginFemale Spermatheca    133.8 Adult InseminatedFemale Spermatheca    146.7 Adult Ovary    283.4 Adult Testis    139 Adult Male Accessory Gland    137.9 Adult Carcass    140.6 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    118.225 Larval Midgut    250.8 Larval Hindgut    243.7 Larval Malpighian Tubules    357.6 Larval Fat Body    167.6 Larval Salivary Gland    228.3 Larval Trachea    293.65 Larval Carcass    231.7 Adult Head    160.4 Adult Eye    161.35 Adult Brain    145.1 Adult Thoracic-Abdominal Ganglion    184.4 Adult Crop    339.3 Adult Midgut    230.8 Adult Hindgut    255 Adult Malpighian Tubules    416.6 Adult Fat Body    94 Adult Salivary Gland    173.5 Adult Heart    147.6 Adult VirginFemale Spermatheca    133.8 Adult InseminatedFemale Spermatheca    146.7 Adult Ovary    283.4 Adult Testis    139 Adult Male Accessory Gland    137.9 Adult Carcass    140.6 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    118.225 Larval Midgut    250.8 Larval Hindgut    243.7 Larval Malpighian Tubules    357.6 Larval Fat Body    167.6 Larval Salivary Gland    228.3 Larval Trachea    293.65 Larval Carcass    231.7 Adult Head    160.4 Adult Eye    161.35 Adult Brain    145.1 Adult Thoracic-Abdominal Ganglion    184.4 Adult Crop    339.3 Adult Midgut    230.8 Adult Hindgut    255 Adult Malpighian Tubules    416.6 Adult Fat Body    94 Adult Salivary Gland    173.5 Adult Heart    147.6 Adult VirginFemale Spermatheca    133.8 Adult InseminatedFemale Spermatheca    146.7 Adult Ovary    283.4 Adult Testis    139 Adult Male Accessory Gland    137.9 Adult Carcass    140.6 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    160.4   NA  Eye    161.35   NA  Brain    145.1   118.225  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    184.4   NA  Crop    339.3   250.8  Midgut    230.8   243.7  Hindgut    255   357.6  Malpighian Tubules    416.6   167.6  Fat Body    94   228.3  Salivary Gland    173.5   NA  Heart    147.6   293.65  Trachea    NA   NA  VirginFemale Spermatheca    133.8   NA  InseminatedFemale Spermatheca    146.7   NA  Ovary    283.4   NA  Testis    139   NA  Male Accessory Gland    137.9   231.7  Carcass    140.6 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    51 central nervous system, larvae L3    33 central nervous system, pupae P8    17 head, virgin 1-day female    20 head, virgin 4-day female    25 head, virgin 20-day female    27 head, mated 1-day female    22 head, mated 4-day female    18 head, mated 20-day female    24 head, mated 1-day male    35 head, mated 4-day male    27 head, mated 20-day male    44 salivary gland, larvae L3 wandering    36 salivary gland, white prepupae    150 digestive system, larvae L3 wandering    82 digestive system, 1-day adult    52 digestive system, 4-day adult    39 digestive system, 20-day adult    52 fat body, larvae L3 wandering    28 fat body, white prepupae    30 fat body, pupae P8    29 carcass, larvae L3 wandering    49 carcass, 1-day adult    24 carcass, 4-day adult    22 carcass, 20-day adult    36 ovary, virgin 4-day female    65 ovary, mated 4-day female    58 testis, mated 4-day male    63 accessory gland, mated 4-day male    28 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (51) central nervous system, larvae L3  (33) central nervous system, pupae P8    17 head, virgin 1-day female    20 head, virgin 4-day female    25 head, virgin 20-day female  (27) head, mated 1-day female    22 head, mated 4-day female    18 head, mated 20-day female    24 head, mated 1-day male  (35) head, mated 4-day male  (27) head, mated 20-day male  (44) salivary gland, larvae L3 wandering  (36) salivary gland, white prepupae  (150) digestive system, larvae L3 wandering  (82) digestive system, 1-day adult  (52) digestive system, 4-day adult  (39) digestive system, 20-day adult  (52) fat body, larvae L3 wandering  (28) fat body, white prepupae  (30) fat body, pupae P8  (29) carcass, larvae L3 wandering  (49) carcass, 1-day adult    24 carcass, 4-day adult    22 carcass, 20-day adult  (36) ovary, virgin 4-day female  (65) ovary, mated 4-day female  (58) testis, mated 4-day male  (63) accessory gland, mated 4-day male  (28) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    51 central nervous system, larvae L3    33 central nervous system, pupae P8    17 head, virgin 1-day female    20 head, virgin 4-day female    25 head, virgin 20-day female    27 head, mated 1-day female    22 head, mated 4-day female    18 head, mated 20-day female    24 head, mated 1-day male    35 head, mated 4-day male    27 head, mated 20-day male    44 salivary gland, larvae L3 wandering    36 salivary gland, white prepupae  (150) digestive system, larvae L3 wandering    82 digestive system, 1-day adult    52 digestive system, 4-day adult    39 digestive system, 20-day adult    52 fat body, larvae L3 wandering    28 fat body, white prepupae    30 fat body, pupae P8    29 carcass, larvae L3 wandering    49 carcass, 1-day adult    24 carcass, 4-day adult    22 carcass, 20-day adult    36 ovary, virgin 4-day female    65 ovary, mated 4-day female    58 testis, mated 4-day male    63 accessory gland, mated 4-day male    28 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    51 central nervous system, larvae L3    33 central nervous system, pupae P8    17 head, virgin 1-day female    20 head, virgin 4-day female    25 head, virgin 20-day female    27 head, mated 1-day female    22 head, mated 4-day female    18 head, mated 20-day female    24 head, mated 1-day male    35 head, mated 4-day male    27 head, mated 20-day male    44 salivary gland, larvae L3 wandering    36 salivary gland, white prepupae    150 digestive system, larvae L3 wandering    82 digestive system, 1-day adult    52 digestive system, 4-day adult    39 digestive system, 20-day adult    52 fat body, larvae L3 wandering    28 fat body, white prepupae    30 fat body, pupae P8    29 carcass, larvae L3 wandering    49 carcass, 1-day adult    24 carcass, 4-day adult    22 carcass, 20-day adult    36 ovary, virgin 4-day female    65 ovary, mated 4-day female    58 testis, mated 4-day male    63 accessory gland, mated 4-day male    28 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    51 central nervous system, larvae L3    33 central nervous system, pupae P8    17 head, virgin 1-day female    20 head, virgin 4-day female    25 head, virgin 20-day female    27 head, mated 1-day female    22 head, mated 4-day female    18 head, mated 20-day female    24 head, mated 1-day male    35 head, mated 4-day male    27 head, mated 20-day male    44 salivary gland, larvae L3 wandering    36 salivary gland, white prepupae    150 digestive system, larvae L3 wandering    82 digestive system, 1-day adult    52 digestive system, 4-day adult    39 digestive system, 20-day adult    52 fat body, larvae L3 wandering    28 fat body, white prepupae    30 fat body, pupae P8    29 carcass, larvae L3 wandering    49 carcass, 1-day adult    24 carcass, 4-day adult    22 carcass, 20-day adult    36 ovary, virgin 4-day female    65 ovary, mated 4-day female    58 testis, mated 4-day male    63 accessory gland, mated 4-day male    28 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (51) central nervous system, larvae L3  (33) central nervous system, pupae P8    17 head, virgin 1-day female    20 head, virgin 4-day female    25 head, virgin 20-day female  27 head, mated 1-day female    22 head, mated 4-day female    18 head, mated 20-day female    24 head, mated 1-day male  (35) head, mated 4-day male  27 head, mated 20-day male  (44) salivary gland, larvae L3 wandering  (36) salivary gland, white prepupae  (150) digestive system, larvae L3 wandering  (82) digestive system, 1-day adult  (52) digestive system, 4-day adult  (39) digestive system, 20-day adult  (52) fat body, larvae L3 wandering  28 fat body, white prepupae  30 fat body, pupae P8  29 carcass, larvae L3 wandering  (49) carcass, 1-day adult    24 carcass, 4-day adult    22 carcass, 20-day adult  (36) ovary, virgin 4-day female  (65) ovary, mated 4-day female  (58) testis, mated 4-day male  (63) accessory gland, mated 4-day male  28 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    51 central nervous system, larvae L3    33 central nervous system, pupae P8    17 head, virgin 1-day female    20 head, virgin 4-day female    25 head, virgin 20-day female    27 head, mated 1-day female    22 head, mated 4-day female    18 head, mated 20-day female    24 head, mated 1-day male    35 head, mated 4-day male    27 head, mated 20-day male    44 salivary gland, larvae L3 wandering    36 salivary gland, white prepupae  (150) digestive system, larvae L3 wandering    82 digestive system, 1-day adult    52 digestive system, 4-day adult    39 digestive system, 20-day adult    52 fat body, larvae L3 wandering    28 fat body, white prepupae    30 fat body, pupae P8    29 carcass, larvae L3 wandering    49 carcass, 1-day adult    24 carcass, 4-day adult    22 carcass, 20-day adult    36 ovary, virgin 4-day female    65 ovary, mated 4-day female    58 testis, mated 4-day male    63 accessory gland, mated 4-day male    28 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    51 central nervous system, larvae L3    33 central nervous system, pupae P8    17 head, virgin 1-day female    20 head, virgin 4-day female    25 head, virgin 20-day female    27 head, mated 1-day female    22 head, mated 4-day female    18 head, mated 20-day female    24 head, mated 1-day male    35 head, mated 4-day male    27 head, mated 20-day male    44 salivary gland, larvae L3 wandering    36 salivary gland, white prepupae    150 digestive system, larvae L3 wandering    82 digestive system, 1-day adult    52 digestive system, 4-day adult    39 digestive system, 20-day adult    52 fat body, larvae L3 wandering    28 fat body, white prepupae    30 fat body, pupae P8    29 carcass, larvae L3 wandering    49 carcass, 1-day adult    24 carcass, 4-day adult    22 carcass, 20-day adult    36 ovary, virgin 4-day female    65 ovary, mated 4-day female    58 testis, mated 4-day male    63 accessory gland, mated 4-day male    28 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 18-24 hour embryonic stages, during early pupal stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    40 embryo 02-04hr    12 embryo 04-06hr    17 embryo 06-08hr    20 embryo 08-10hr    22 embryo 10-12hr    41 embryo 12-14hr    31 embryo 14-16hr    34 embryo 16-18hr    41 embryo 18-20hr    63 embryo 20-22hr    48 embryo 22-24hr    52 larva L1    28 larva L2    20 larva L3 12hr old    17 larva L3 puffstage 1-2    25 larva L3 puffstage 3-6    34 larva L3 puffstage 7-9    47 white prepupae new    60 white prepupae 12hr    44 white prepupae 24hr    37 pupae 2d postWPP    26 pupae 3d postWPP    23 pupae 4d postWPP    26 adult male 01day    23 adult male 05day    30 adult male 30day    34 adult female 01day    25 adult female 05day    30 adult female 30day    33 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (40) embryo 02-04hr    12 embryo 04-06hr    17 embryo 06-08hr    20 embryo 08-10hr    22 embryo 10-12hr  (41) embryo 12-14hr  (31) embryo 14-16hr  (34) embryo 16-18hr  (41) embryo 18-20hr  (63) embryo 20-22hr  (48) embryo 22-24hr  (52) larva L1  (28) larva L2    20 larva L3 12hr old    17 larva L3 puffstage 1-2    25 larva L3 puffstage 3-6  (34) larva L3 puffstage 7-9  (47) white prepupae new  (60) white prepupae 12hr  (44) white prepupae 24hr  (37) pupae 2d postWPP  26 pupae 3d postWPP    23 pupae 4d postWPP  26 adult male 01day    23 adult male 05day  (30) adult male 30day  (34) adult female 01day    25 adult female 05day  (30) adult female 30day  (33) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    40 embryo 02-04hr    12 embryo 04-06hr    17 embryo 06-08hr    20 embryo 08-10hr    22 embryo 10-12hr    41 embryo 12-14hr    31 embryo 14-16hr    34 embryo 16-18hr    41 embryo 18-20hr    63 embryo 20-22hr    48 embryo 22-24hr    52 larva L1    28 larva L2    20 larva L3 12hr old    17 larva L3 puffstage 1-2    25 larva L3 puffstage 3-6    34 larva L3 puffstage 7-9    47 white prepupae new    60 white prepupae 12hr    44 white prepupae 24hr    37 pupae 2d postWPP    26 pupae 3d postWPP    23 pupae 4d postWPP    26 adult male 01day    23 adult male 05day    30 adult male 30day    34 adult female 01day    25 adult female 05day    30 adult female 30day    33 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    40 embryo 02-04hr    12 embryo 04-06hr    17 embryo 06-08hr    20 embryo 08-10hr    22 embryo 10-12hr    41 embryo 12-14hr    31 embryo 14-16hr    34 embryo 16-18hr    41 embryo 18-20hr    63 embryo 20-22hr    48 embryo 22-24hr    52 larva L1    28 larva L2    20 larva L3 12hr old    17 larva L3 puffstage 1-2    25 larva L3 puffstage 3-6    34 larva L3 puffstage 7-9    47 white prepupae new    60 white prepupae 12hr    44 white prepupae 24hr    37 pupae 2d postWPP    26 pupae 3d postWPP    23 pupae 4d postWPP    26 adult male 01day    23 adult male 05day    30 adult male 30day    34 adult female 01day    25 adult female 05day    30 adult female 30day    33 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    40 embryo 02-04hr    12 embryo 04-06hr    17 embryo 06-08hr    20 embryo 08-10hr    22 embryo 10-12hr    41 embryo 12-14hr    31 embryo 14-16hr    34 embryo 16-18hr    41 embryo 18-20hr    63 embryo 20-22hr    48 embryo 22-24hr    52 larva L1    28 larva L2    20 larva L3 12hr old    17 larva L3 puffstage 1-2    25 larva L3 puffstage 3-6    34 larva L3 puffstage 7-9    47 white prepupae new    60 white prepupae 12hr    44 white prepupae 24hr    37 pupae 2d postWPP    26 pupae 3d postWPP    23 pupae 4d postWPP    26 adult male 01day    23 adult male 05day    30 adult male 30day    34 adult female 01day    25 adult female 05day    30 adult female 30day    33 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (40) embryo 02-04hr    12 embryo 04-06hr    17 embryo 06-08hr    20 embryo 08-10hr    22 embryo 10-12hr  (41) embryo 12-14hr  31 embryo 14-16hr  (34) embryo 16-18hr  (41) embryo 18-20hr  (63) embryo 20-22hr  (48) embryo 22-24hr  (52) larva L1  28 larva L2    20 larva L3 12hr old    17 larva L3 puffstage 1-2    25 larva L3 puffstage 3-6  (34) larva L3 puffstage 7-9  (47) white prepupae new  (60) white prepupae 12hr  (44) white prepupae 24hr  (37) pupae 2d postWPP  26 pupae 3d postWPP    23 pupae 4d postWPP  26 adult male 01day    23 adult male 05day  30 adult male 30day  (34) adult female 01day    25 adult female 05day  30 adult female 30day  (33) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    40 embryo 02-04hr    12 embryo 04-06hr    17 embryo 06-08hr    20 embryo 08-10hr    22 embryo 10-12hr    41 embryo 12-14hr    31 embryo 14-16hr    34 embryo 16-18hr    41 embryo 18-20hr    63 embryo 20-22hr    48 embryo 22-24hr    52 larva L1    28 larva L2    20 larva L3 12hr old    17 larva L3 puffstage 1-2    25 larva L3 puffstage 3-6    34 larva L3 puffstage 7-9    47 white prepupae new    60 white prepupae 12hr    44 white prepupae 24hr    37 pupae 2d postWPP    26 pupae 3d postWPP    23 pupae 4d postWPP    26 adult male 01day    23 adult male 05day    30 adult male 30day    34 adult female 01day    25 adult female 05day    30 adult female 30day    33 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    40 embryo 02-04hr    12 embryo 04-06hr    17 embryo 06-08hr    20 embryo 08-10hr    22 embryo 10-12hr    41 embryo 12-14hr    31 embryo 14-16hr    34 embryo 16-18hr    41 embryo 18-20hr    63 embryo 20-22hr    48 embryo 22-24hr    52 larva L1    28 larva L2    20 larva L3 12hr old    17 larva L3 puffstage 1-2    25 larva L3 puffstage 3-6    34 larva L3 puffstage 7-9    47 white prepupae new    60 white prepupae 12hr    44 white prepupae 24hr    37 pupae 2d postWPP    26 pupae 3d postWPP    23 pupae 4d postWPP    26 adult male 01day    23 adult male 05day    30 adult male 30day    34 adult female 01day    25 adult female 05day    30 adult female 30day    33 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    144 Schneider line 2 Sg4    89 embryonic 1182-4H    87 embryonic GM2    63 embryonic Kc167    56 embryonic S1    124 embryonic S3    64 leg disc CME L1    159 wing disc CME-W2    60 wing disc ML-DmD8    43 wing disc ML-DmD9    94 wing disc ML-DmD16-c3    87 wing disc ML-DmD21    136 wing disc ML-DmD32    118 haltere disc ML-DmD17-c3    59 eye-antennal disc ML-DmD11    67 antennal disc ML-DmD20-c5    70 mixed discs ML-DmD4-c1    59 CNS ML-DmBG1-c1    112 CNS ML-DmBG2-c2    134 tumorous blood cells mbn2    73 ovary fGS/OSS    73 ovary OSC    49 ovary OSS    35 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (144) Schneider line 2 Sg4  (89) embryonic 1182-4H  (87) embryonic GM2  (63) embryonic Kc167  (56) embryonic S1  (124) embryonic S3  (64) leg disc CME L1  (159) wing disc CME-W2  (60) wing disc ML-DmD8  (43) wing disc ML-DmD9  (94) wing disc ML-DmD16-c3  (87) wing disc ML-DmD21  (136) wing disc ML-DmD32  (118) haltere disc ML-DmD17-c3  (59) eye-antennal disc ML-DmD11  (67) antennal disc ML-DmD20-c5  (70) mixed discs ML-DmD4-c1  (59) CNS ML-DmBG1-c1  (112) CNS ML-DmBG2-c2  (134) tumorous blood cells mbn2  (73) ovary fGS/OSS  (73) ovary OSC  (49) ovary OSS  (35) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+  (144) Schneider line 2 Sg4    89 embryonic 1182-4H    87 embryonic GM2    63 embryonic Kc167    56 embryonic S1  (124) embryonic S3    64 leg disc CME L1  (159) wing disc CME-W2    60 wing disc ML-DmD8    43 wing disc ML-DmD9    94 wing disc ML-DmD16-c3    87 wing disc ML-DmD21  (136) wing disc ML-DmD32  (118) haltere disc ML-DmD17-c3    59 eye-antennal disc ML-DmD11    67 antennal disc ML-DmD20-c5    70 mixed discs ML-DmD4-c1    59 CNS ML-DmBG1-c1  (112) CNS ML-DmBG2-c2  (134) tumorous blood cells mbn2    73 ovary fGS/OSS    73 ovary OSC    49 ovary OSS    35 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    144 Schneider line 2 Sg4    89 embryonic 1182-4H    87 embryonic GM2    63 embryonic Kc167    56 embryonic S1    124 embryonic S3    64 leg disc CME L1    159 wing disc CME-W2    60 wing disc ML-DmD8    43 wing disc ML-DmD9    94 wing disc ML-DmD16-c3    87 wing disc ML-DmD21    136 wing disc ML-DmD32    118 haltere disc ML-DmD17-c3    59 eye-antennal disc ML-DmD11    67 antennal disc ML-DmD20-c5    70 mixed discs ML-DmD4-c1    59 CNS ML-DmBG1-c1    112 CNS ML-DmBG2-c2    134 tumorous blood cells mbn2    73 ovary fGS/OSS    73 ovary OSC    49 ovary OSS    35 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    144 Schneider line 2 Sg4    89 embryonic 1182-4H    87 embryonic GM2    63 embryonic Kc167    56 embryonic S1    124 embryonic S3    64 leg disc CME L1    159 wing disc CME-W2    60 wing disc ML-DmD8    43 wing disc ML-DmD9    94 wing disc ML-DmD16-c3    87 wing disc ML-DmD21    136 wing disc ML-DmD32    118 haltere disc ML-DmD17-c3    59 eye-antennal disc ML-DmD11    67 antennal disc ML-DmD20-c5    70 mixed discs ML-DmD4-c1    59 CNS ML-DmBG1-c1    112 CNS ML-DmBG2-c2    134 tumorous blood cells mbn2    73 ovary fGS/OSS    73 ovary OSC    49 ovary OSS    35 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (144) Schneider line 2 Sg4  (89) embryonic 1182-4H  (87) embryonic GM2  (63) embryonic Kc167  (56) embryonic S1  (124) embryonic S3  (64) leg disc CME L1  (159) wing disc CME-W2  (60) wing disc ML-DmD8  (43) wing disc ML-DmD9  (94) wing disc ML-DmD16-c3  (87) wing disc ML-DmD21  (136) wing disc ML-DmD32  (118) haltere disc ML-DmD17-c3  (59) eye-antennal disc ML-DmD11  (67) antennal disc ML-DmD20-c5  (70) mixed discs ML-DmD4-c1  (59) CNS ML-DmBG1-c1  (112) CNS ML-DmBG2-c2  (134) tumorous blood cells mbn2  (73) ovary fGS/OSS  (73) ovary OSC  (49) ovary OSS  (35) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+  (144) Schneider line 2 Sg4    89 embryonic 1182-4H    87 embryonic GM2    63 embryonic Kc167    56 embryonic S1  124 embryonic S3    64 leg disc CME L1  (159) wing disc CME-W2    60 wing disc ML-DmD8    43 wing disc ML-DmD9    94 wing disc ML-DmD16-c3    87 wing disc ML-DmD21  136 wing disc ML-DmD32  118 haltere disc ML-DmD17-c3    59 eye-antennal disc ML-DmD11    67 antennal disc ML-DmD20-c5    70 mixed discs ML-DmD4-c1    59 CNS ML-DmBG1-c1  112 CNS ML-DmBG2-c2  134 tumorous blood cells mbn2    73 ovary fGS/OSS    73 ovary OSC    49 ovary OSS    35 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    144 Schneider line 2 Sg4    89 embryonic 1182-4H    87 embryonic GM2    63 embryonic Kc167    56 embryonic S1    124 embryonic S3    64 leg disc CME L1    159 wing disc CME-W2    60 wing disc ML-DmD8    43 wing disc ML-DmD9    94 wing disc ML-DmD16-c3    87 wing disc ML-DmD21    136 wing disc ML-DmD32    118 haltere disc ML-DmD17-c3    59 eye-antennal disc ML-DmD11    67 antennal disc ML-DmD20-c5    70 mixed discs ML-DmD4-c1    59 CNS ML-DmBG1-c1    112 CNS ML-DmBG2-c2    134 tumorous blood cells mbn2    73 ovary fGS/OSS    73 ovary OSC    49 ovary OSS    35 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    41 cold shock, 4-day adult    34 heat shock, 4-day adult    35 Cadmium 50 mM 6 hrs, larvae L3    27 Cadmium 50 mM 12 hrs, larvae L3    34 Cadmium 50 mM 48 hrs, 4-day adult    39 Cadmium 100 mM 48 hrs, 4-day adult    76 Copper 0.5 mM 12 hrs, larvae L3    38 Copper 15 mM 48 hrs, 4-day adult    35 Zinc 5 mM 12 hrs, larvae L3    40 Zinc 4.5 mM 48 hrs, 4-day adult    33 Ethanol 2.5% 3 hrs, larvae L3    58 Ethanol 5% 3 hrs, larvae L3    40 Ethanol 10% 3 hrs, larvae L3    35 Caffeine 1.5 mg/ml 4 hrs, larvae L3    32 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    53 Caffeine 25 mg/ml 48 hrs, 4-day adult    36 Paraquat 5 mM 48 hrs, 4-day adult    40 Paraquat 10 mM 48 hrs, 4-day adult    44 Rotenone 2 μg 12 hrs, larvae L3    33 Rotenone 8 μg 12 hrs, larvae L3    39 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  (41) cold shock, 4-day adult  (34) heat shock, 4-day adult  (35) Cadmium 50 mM 6 hrs, larvae L3  (27) Cadmium 50 mM 12 hrs, larvae L3  (34) Cadmium 50 mM 48 hrs, 4-day adult  (39) Cadmium 100 mM 48 hrs, 4-day adult  (76) Copper 0.5 mM 12 hrs, larvae L3  (38) Copper 15 mM 48 hrs, 4-day adult  (35) Zinc 5 mM 12 hrs, larvae L3  (40) Zinc 4.5 mM 48 hrs, 4-day adult  (33) Ethanol 2.5% 3 hrs, larvae L3  (58) Ethanol 5% 3 hrs, larvae L3  (40) Ethanol 10% 3 hrs, larvae L3  (35) Caffeine 1.5 mg/ml 4 hrs, larvae L3  (32) Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (53) Caffeine 25 mg/ml 48 hrs, 4-day adult  (36) Paraquat 5 mM 48 hrs, 4-day adult  (40) Paraquat 10 mM 48 hrs, 4-day adult  (44) Rotenone 2 μg 12 hrs, larvae L3  (33) Rotenone 8 μg 12 hrs, larvae L3  (39) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    41 cold shock, 4-day adult    34 heat shock, 4-day adult    35 Cadmium 50 mM 6 hrs, larvae L3    27 Cadmium 50 mM 12 hrs, larvae L3    34 Cadmium 50 mM 48 hrs, 4-day adult    39 Cadmium 100 mM 48 hrs, 4-day adult    76 Copper 0.5 mM 12 hrs, larvae L3    38 Copper 15 mM 48 hrs, 4-day adult    35 Zinc 5 mM 12 hrs, larvae L3    40 Zinc 4.5 mM 48 hrs, 4-day adult    33 Ethanol 2.5% 3 hrs, larvae L3    58 Ethanol 5% 3 hrs, larvae L3    40 Ethanol 10% 3 hrs, larvae L3    35 Caffeine 1.5 mg/ml 4 hrs, larvae L3    32 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    53 Caffeine 25 mg/ml 48 hrs, 4-day adult    36 Paraquat 5 mM 48 hrs, 4-day adult    40 Paraquat 10 mM 48 hrs, 4-day adult    44 Rotenone 2 μg 12 hrs, larvae L3    33 Rotenone 8 μg 12 hrs, larvae L3    39 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    41 cold shock, 4-day adult    34 heat shock, 4-day adult    35 Cadmium 50 mM 6 hrs, larvae L3    27 Cadmium 50 mM 12 hrs, larvae L3    34 Cadmium 50 mM 48 hrs, 4-day adult    39 Cadmium 100 mM 48 hrs, 4-day adult    76 Copper 0.5 mM 12 hrs, larvae L3    38 Copper 15 mM 48 hrs, 4-day adult    35 Zinc 5 mM 12 hrs, larvae L3    40 Zinc 4.5 mM 48 hrs, 4-day adult    33 Ethanol 2.5% 3 hrs, larvae L3    58 Ethanol 5% 3 hrs, larvae L3    40 Ethanol 10% 3 hrs, larvae L3    35 Caffeine 1.5 mg/ml 4 hrs, larvae L3    32 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    53 Caffeine 25 mg/ml 48 hrs, 4-day adult    36 Paraquat 5 mM 48 hrs, 4-day adult    40 Paraquat 10 mM 48 hrs, 4-day adult    44 Rotenone 2 μg 12 hrs, larvae L3    33 Rotenone 8 μg 12 hrs, larvae L3    39 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    41 cold shock, 4-day adult    34 heat shock, 4-day adult    35 Cadmium 50 mM 6 hrs, larvae L3    27 Cadmium 50 mM 12 hrs, larvae L3    34 Cadmium 50 mM 48 hrs, 4-day adult    39 Cadmium 100 mM 48 hrs, 4-day adult    76 Copper 0.5 mM 12 hrs, larvae L3    38 Copper 15 mM 48 hrs, 4-day adult    35 Zinc 5 mM 12 hrs, larvae L3    40 Zinc 4.5 mM 48 hrs, 4-day adult    33 Ethanol 2.5% 3 hrs, larvae L3    58 Ethanol 5% 3 hrs, larvae L3    40 Ethanol 10% 3 hrs, larvae L3    35 Caffeine 1.5 mg/ml 4 hrs, larvae L3    32 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    53 Caffeine 25 mg/ml 48 hrs, 4-day adult    36 Paraquat 5 mM 48 hrs, 4-day adult    40 Paraquat 10 mM 48 hrs, 4-day adult    44 Rotenone 2 μg 12 hrs, larvae L3    33 Rotenone 8 μg 12 hrs, larvae L3    39 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  (41) cold shock, 4-day adult  (34) heat shock, 4-day adult  (35) Cadmium 50 mM 6 hrs, larvae L3  27 Cadmium 50 mM 12 hrs, larvae L3  (34) Cadmium 50 mM 48 hrs, 4-day adult  (39) Cadmium 100 mM 48 hrs, 4-day adult  (76) Copper 0.5 mM 12 hrs, larvae L3  (38) Copper 15 mM 48 hrs, 4-day adult  (35) Zinc 5 mM 12 hrs, larvae L3  (40) Zinc 4.5 mM 48 hrs, 4-day adult  (33) Ethanol 2.5% 3 hrs, larvae L3  (58) Ethanol 5% 3 hrs, larvae L3  (40) Ethanol 10% 3 hrs, larvae L3  (35) Caffeine 1.5 mg/ml 4 hrs, larvae L3  32 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (53) Caffeine 25 mg/ml 48 hrs, 4-day adult  (36) Paraquat 5 mM 48 hrs, 4-day adult  (40) Paraquat 10 mM 48 hrs, 4-day adult  (44) Rotenone 2 μg 12 hrs, larvae L3  (33) Rotenone 8 μg 12 hrs, larvae L3  (39) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    41 cold shock, 4-day adult    34 heat shock, 4-day adult    35 Cadmium 50 mM 6 hrs, larvae L3    27 Cadmium 50 mM 12 hrs, larvae L3    34 Cadmium 50 mM 48 hrs, 4-day adult    39 Cadmium 100 mM 48 hrs, 4-day adult    76 Copper 0.5 mM 12 hrs, larvae L3    38 Copper 15 mM 48 hrs, 4-day adult    35 Zinc 5 mM 12 hrs, larvae L3    40 Zinc 4.5 mM 48 hrs, 4-day adult    33 Ethanol 2.5% 3 hrs, larvae L3    58 Ethanol 5% 3 hrs, larvae L3    40 Ethanol 10% 3 hrs, larvae L3    35 Caffeine 1.5 mg/ml 4 hrs, larvae L3    32 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    53 Caffeine 25 mg/ml 48 hrs, 4-day adult    36 Paraquat 5 mM 48 hrs, 4-day adult    40 Paraquat 10 mM 48 hrs, 4-day adult    44 Rotenone 2 μg 12 hrs, larvae L3    33 Rotenone 8 μg 12 hrs, larvae L3    39 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    41 cold shock, 4-day adult    34 heat shock, 4-day adult    35 Cadmium 50 mM 6 hrs, larvae L3    27 Cadmium 50 mM 12 hrs, larvae L3    34 Cadmium 50 mM 48 hrs, 4-day adult    39 Cadmium 100 mM 48 hrs, 4-day adult    76 Copper 0.5 mM 12 hrs, larvae L3    38 Copper 15 mM 48 hrs, 4-day adult    35 Zinc 5 mM 12 hrs, larvae L3    40 Zinc 4.5 mM 48 hrs, 4-day adult    33 Ethanol 2.5% 3 hrs, larvae L3    58 Ethanol 5% 3 hrs, larvae L3    40 Ethanol 10% 3 hrs, larvae L3    35 Caffeine 1.5 mg/ml 4 hrs, larvae L3    32 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    53 Caffeine 25 mg/ml 48 hrs, 4-day adult    36 Paraquat 5 mM 48 hrs, 4-day adult    40 Paraquat 10 mM 48 hrs, 4-day adult    44 Rotenone 2 μg 12 hrs, larvae L3    33 Rotenone 8 μg 12 hrs, larvae L3    39 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_27 mE1_20_mRNA_expression_cluster_03
External Data & Images
Linkouts	BDGP expression data • LD25202 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0001291 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG2275-RB
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal (with Df(2R)X1) Jra76-19 lethal | embryonic stage, with Scer\GAL4pnr-MD237 JraAsp.B.Scer\UAS lethal | embryonic stage | recessive Jra1 Jra76-19 JradsRNA.cNa JraIA109 lethal | prepupal stage, with Scer\GAL4ey.PH JraAsp.B.Scer\UAS partially lethal - majority die | pupal stage, with Scer\GAL4pnr-MD237 JraGD4400 Other Phenotypes Allele hypoactive | adult stage, with Scer\GAL4repo.PL, Scer\GAL4repo JraKK100582 neuroanatomy defective, with Scer\GAL4D42, Scer\GAL80Cha.PK JraJbz.Scer\UAS neuroanatomy defective, with Scer\GAL4elav-C155 JraJbz.Scer\UAS neuroanatomy defective | somatic clone | third instar larval stage, with Scer\GAL4elav.PH JraJbz.Scer\UAS neurophysiology defective, with Scer\GAL4elav-C155 JraJbz.Scer\UAS planar polarity defective, with Scer\GAL4pnr-MD237 JraGD4400 planar polarity defective | dominant JraAsp.hs.sev planar polarity defective | somatic clone Jra76-19 JraIA109 visible JraAsp.hs.sev visible, with Scer\GAL4pnr-MD237 JradsRNA.Scer\UAS JraGD4400 visible | dominant JraAsp.hs.sev wild-type Jra+t5.2 Phenotype manifest in Allele adult thorax, with Scer\GAL4pnr-MD237 JradsRNA.Scer\UAS bouton, with Scer\GAL4elav-C155 JraJbz.Scer\UAS commissure, with Scer\GAL4sim.PS JraAsp.Scer\UAS cone cell JraAsp.hs.sev dendrite, with Scer\GAL4D42, Scer\GAL80Cha.PK JraJbz.Scer\UAS dorsal appendage | maternal effect | somatic clone JraIA109 dorsal closure embryo JraIA109 embryo | anterior | dorsal JraIA109 embryonic/first instar larval cuticle | dorsal JradsRNA.cNa embryonic/first instar larval cuticle | heat sensitive JraAsp.hs.sev embryonic/larval neuromuscular junction | somatic clone, with Scer\GAL4elav.PH JraJbz.Scer\UAS embryonic dorsal epidermis Jra1 Jra76-19 JraIA109 embryonic epidermis | dorsal JraIA109 epidermis | dorsal JraIA109 eye JraAla.hs.sev JraAsp.hs.sev JrabZIP.hs.sev.T:Ivir\HA1 eye, with Scer\GAL4unspecified JraJbz.Scer\UAS eye disc JraAsp.hs.sev eye photoreceptor cell JraAla.hs.sev JrabZIP.hs.sev.T:Ivir\HA1 eye photoreceptor cell | ectopic JraAsp.hs.sev head Jra76-19 JraIA109 indirect flight muscle motor neuron MN5, with Scer\GAL4D42, Scer\GAL80Cha.PK JraJbz.Scer\UAS mesothoracic tergum, with Scer\GAL4pnr-MD237 JraGD4400 micropyle | maternal effect | somatic clone JraIA109 midline primordium JraIA109 neuromuscular junction | larval stage, with Scer\GAL4elav-C155 JraJbz.Scer\UAS NMJ bouton | somatic clone, with Scer\GAL4elav.PH JraJbz.Scer\UAS ommatidial precursor cluster JraAsp.hs.sev ommatidium JraAsp.hs.sev JrabZIP.hs.sev.T:Ivir\HA1 ommatidium | somatic clone Jra76-19 JraIA109 photoreceptor cell JraAD.hs.sev.T:Hsim\VP16 photoreceptor cell R1 JraS82A.T92A.T107A.hs.sev photoreceptor cell R2 JraS82A.T92A.T107A.hs.sev photoreceptor cell R3 JraS82A.T92A.T107A.hs.sev photoreceptor cell R3 | somatic clone Jra76-19 JraIA109 photoreceptor cell R4 JraAsp.hs.sev JraS82A.T92A.T107A.hs.sev photoreceptor cell R4 | somatic clone | supernumerary Jra76-19 JraIA109 photoreceptor cell R5 JraS82A.T92A.T107A.hs.sev photoreceptor cell R6 JraS82A.T92A.T107A.hs.sev photoreceptor cell R7 JraAla.hs.sev JraAsp.hs.sev JraS82A.T92A.T107A.hs.sev photoreceptor cell R7 | supernumerary JraAsp.hs.sev rhabdomere JraAsp.hs.sev
Classical Alleles ( 5 )
For All Classical Alleles Show
Allele of Jra Class Mutagen Stocks Known lesion JraIA109 loss of function allele, amorphic allele - genetic evidence ethyl methanesulfonate 2 Yes Jra76-19 amorphic allele - genetic evidence ethyl methanesulfonate diepoxybutane gamma ray 1 Yes Jra1 loss of function allele ethyl methanesulfonate 0 Yes Jra3 0 -- JraRC46 0 --
Alleles Carried on Transgenic Constructs ( 26 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Jra Class Mutagen Stocks Known lesion JraGD4400 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes JraJF01184 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes JraKK100582 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes JraScer\UAS.cEa in vitro construct - regulatory fusion 1 Yes Jra+t5.2 in vitro construct - genomic fragment 0 Yes JraAD.hs.sev.T:Hsim\VP16 in vitro construct - coding region fusion 0 Yes JraAla.B.Scer\UAS in vitro construct - regulatory fusion 0 Yes JraAla.hs.sev antimorphic allele - genetic evidence in vitro construct - regulatory fusion in vitro construct - amino acid replacement 0 Yes JraAsp.B.Scer\UAS in vitro construct - regulatory fusion 0 Yes JraAsp.hs.sev in vitro construct - regulatory fusion in vitro construct - amino acid replacement 0 Yes JraAsp.Scer\UAS in vitro construct - regulatory fusion 0 Yes JrabZIP.hs.sev.T:Ivir\HA1 antimorphic allele - genetic evidence in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - deletion 0 Yes JracRa in vitro construct 0 Yes JraDN.Scer\UAS in vitro construct - regulatory fusion 0 Yes JradsRNA.cBa.Scer\UAS in vitro construct - regulatory fusion in vitro construct - RNAi 0 Yes JradsRNA.cNa in vitro construct - RNAi 0 Yes JradsRNA.Scer\UAS in vitro construct - regulatory fusion in vitro construct - RNAi 0 Yes Jrafl.hs.sev.T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes Jrahs.sev in vitro construct - regulatory fusion 0 Yes Jrahs.T:Ecol\dam,T:Hsap\MYC in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes JraJbz.Scer\UAS antimorphic allele - genetic evidence in vitro construct - regulatory fusion 0 Yes JraJF01379 in vitro construct - regulatory fusion in vitro construct - RNAi 0 Yes JraNIG.2275R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes JraS82A.T92A.T107A.hs.sev in vitro construct - amino acid replacement in vitro construct - regulatory fusion 0 Yes JraS82D.T92D.T107D.hs.sev in vitro construct - amino acid replacement in vitro construct - regulatory fusion 0 Yes JraScer\UAS.T:Zzzz\TAP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2R)BSC303 (Christensen and Cook, 2007.5.8) Df(2R)E73 (Kockel et al., 1997) Df(2R)X1 (Goldstein et al., 1998, Goldstein et al., 2001, Riesgo-Escovar and Hafen, 1997, Carney et al., 1997)
Not disrupted in	Ab(2R)55-20 (Goldstein et al., 2001) Ab(2R)89-22 (Goldstein et al., 2001) Df(2R)12 (Carney et al., 1997) Df(2R)61-25 (Goldstein et al., 2001) Df(2R)64-10 (Goldstein et al., 2001) Df(2R)71-81 (Goldstein et al., 2001) Df(2R)520 (Goldstein et al., 2001) Df(2R)en-A (Chase and Baker, 1995) Df(2R)en-B (Chase and Baker, 1995) Df(2R)eve (Goldstein et al., 2001) Df(2R)stan2 (Goldstein et al., 2001) Df(2R)X3 (Goldstein et al., 2001, Carney et al., 1997)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD4400} NA P{Jbz} NA P{KK100582} NA P{NIG.2275R} NA P{TRiP.JF01184} NA P{UAS-act-jun} NA P{UAS-Jra.DN} NA P{UAS-Jra.RNAi} NA P{UAS-Jra.TAP} NA P{UAS-Jra} NA P{UAS-jun.B.RNAi} NA P{UAS-junAla.B} NA P{UAS-junAsp.B} NA heat-shock construct P{sE-D-junS82A,T92A,T107A} NA P{sE-D-junS82D,T92D,T107D} NA P{sE-dm-jun} NA P{sE-junAla} NA P{sE-junAsp} NA P{sE-junbZip} NA P{sE-junwt} NA P{VP16-D-jun} NA characterization construct P{hs.sev-Jra::kay.FJF} NA P{hs.sev-Jra::kay.JFJ} NA P{Jra+t5.2} NA P{TRiP.JF01379} NA
Insertions
	Type of insertions Name Expression data
Gene Ontology: Function, Process & Cellular Component ( 24 unique terms )
Terms Based on Experimental Evidence ( 15 terms )
Molecular Function
CV term Evidence References
protein binding inferred from physical interaction with Atf3 (Sekyrova et al., 2010) inferred from physical interaction with chm (Miotto et al., 2006) inferred from physical interaction with Cka (Chen et al., 2002) protein heterodimerization activity inferred from physical interaction with kay (Perkins et al., 1990) sequence-specific DNA binding RNA polymerase II transcription factor activity inferred from direct assay (Perkins et al., 1990)
Biological Process
CV term Evidence References
dorsal appendage formation inferred from mutant phenotype (Suzanne et al., 2001) imaginal disc fusion, thorax closure inferred from mutant phenotype (Cerrato et al., 2006) JNK cascade inferred from genetic interaction with Rac1 AND inferred from genetic interaction with bsk AND inferred from genetic interaction with hep AND inferred from mutant phenotype (Hou et al., 1997) micropyle formation inferred from mutant phenotype (Suzanne et al., 2001) negative regulation of antimicrobial humoral response inferred from genetic interaction with Rel (Kim et al., 2007) inferred from mutant phenotype (Kim et al., 2007) phagocytosis, engulfment inferred from mutant phenotype (Stroschein-Stevenson et al., 2006) positive regulation of transcription from RNA polymerase II promoter inferred from direct assay (Perkins et al., 1990) synaptic growth at neuromuscular junction inferred from genetic interaction with kay (Franciscovich et al., 2008) wound healing inferred from mutant phenotype (Campos et al., 2010)
Cellular Component
CV term Evidence References
cytoplasm inferred from direct assay (Reed et al., 2001) colocalizes_with cytoplasm inferred from direct assay (Jemc et al., 2012) nucleus inferred from direct assay (Reed et al., 2001) colocalizes_with nucleus inferred from direct assay (Jemc et al., 2012) transcription factor complex inferred from physical interaction with kay (Perkins et al., 1990)
Terms Based on Predictions or Assertions ( 12 terms )
Molecular Function
CV term Evidence References
protein heterodimerization activity inferred from sequence or structural similarity (Fassler et al., 2002) sequence-specific DNA binding inferred from electronic annotation with InterPro:IPR004827, InterPro:IPR011616 (FlyBase Curators et al., 2004-) sequence-specific DNA binding transcription factor activity non-traceable author statement (Swiss-Prot Project Members, 1990.11.1) transcription factor binding non-traceable author statement (Swiss-Prot Project Members, 1990.11.1) traceable author statement (Van Aelst and Symons, 2002)
Biological Process
CV term Evidence References
dorsal closure non-traceable author statement (Van Aelst and Symons, 2002) traceable author statement (Noselli and Agnes, 1999, Lamka and Lipshitz, 1999, Harden, 2002) establishment of planar polarity non-traceable author statement (Mlodzik, 2002) JNK cascade traceable author statement (Noselli and Agnes, 1999) MAPK cascade traceable author statement (Harden, 2002) R3/R4 cell fate commitment traceable author statement (Mlodzik, 2002) R7 cell fate commitment traceable author statement (Riesgo-Escovar et al., 1996) regulation of transcription from RNA polymerase II promoter non-traceable author statement (Swiss-Prot Project Members, 1990.11.1)
Cellular Component
CV term Evidence References
nucleus inferred from sequence or structural similarity with UniProtKB:P27921 (FlyBase, 1992-) non-traceable author statement (Swiss-Prot Project Members, 1990.11.1)
Sequence Ontology: Class of Gene
	gene_with_stop_codon_read_through (Jungreis et al., 2011) nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
Jra - 14-3-3ζ anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Jra - Arpc1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Jra - Arpc5 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Jra - Bacc anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Jra - CG10077 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Jra - CG10417 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Jra - CG10641 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Jra - CG13123 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) Jra - CG1407 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Jra - CG14818 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Jra - CG17601 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Jra - CG18178 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Jra - CG2921 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Jra - CG31910 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Jra - CG3817 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Jra - CG3862 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Jra - CG4098 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Jra - CG4707 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Jra - CG6123 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Jra - CG6178 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Jra - CG6701 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Jra - CG6937 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Jra - CG7903 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Jra - CG8314 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Jra - CG8545 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Jra - CG8578 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Jra - CG8735 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Jra - CG8801 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Jra - CG9775 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Jra - CG9977 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Jra - Ect4 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Jra - Gadd34 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Jra - GstO1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Jra - Imp anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Jra - Ipk1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Jra - MED27 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Jra - Mob4 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Jra - Nle anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Jra - Optix anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Jra - Pgam5 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Jra - RFeSP anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Jra - Rab5 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Jra - Ras85D anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Jra - Rbp1-like anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Jra - Rcd5 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Jra - RpL35A anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Jra - RpL7-like anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Jra - RpLP0 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Jra - RpS11 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Jra - RpS12 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Jra - Rpd3 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Jra - Rrp40 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Jra - Rs1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Jra - Rsf1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Jra - SmD2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Jra - Smn anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Jra - Srp54 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Jra - Syp anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Jra - TM9SF4 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Jra - Thiolase anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Jra - Top1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Jra - alt anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Jra - aur anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Jra - cpb anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Jra - ctp anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Jra - dbe anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Jra - eIF6 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Jra - fbl6 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Jra - kay experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) Jra - kst anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Jra - l(1)G0020 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Jra - mRpL43 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Jra - mRpL44 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Jra - mib2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Jra - msk anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Jra - skpA anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Jra - slp1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Jra - α-Spec anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Jra allele Gene References Jra1 dsh (Boutros et al., 1998) Egfr (Kockel et al., 1997) peb (Pickup et al., 2002, Wilk et al., 2004) Rac1 (Fanto et al., 2000) rl (Kockel et al., 1997) sev (Kockel et al., 1997) Jra3 Rac1 (Fanto et al., 2000) Tak1 (Mihaly et al., 2001) Jra76-19 tkv (Hou et al., 1997) JraAsp.hs.sev aop (Treier et al., 1995) bsk (Hou et al., 1997) Btk29A (Tateno et al., 2000) Cka (Chen et al., 2002) hep (Hou et al., 1997) mbl (Begemann et al., 1997) msn (Su et al., 1998) phyl (Treier et al., 1995) pnt (Treier et al., 1995, Weber et al., 2000) Rac1 (Hou et al., 1997) shark (Fernandez et al., 2000) slpr (Stronach and Perrimon, 2002) JrabZIP.hs.sev.T:Ivir\HA1 bsk (Riesgo-Escovar and Hafen, 1997) sev (Bohmann et al., 1994) Jrahs.sev dsh (Boutros et al., 1998) JraIA109 dsh (Boutros et al., 1998) Egfr (Kockel et al., 1997) egr (Igaki et al., 2002) faf (Isaksson et al., 1997) Jra::kay (Ciapponi and Bohmann, 2002) kay (Riesgo-Escovar and Hafen, 1997) mbf1 (Jindra et al., 2004) msn (Paricio et al., 1999) peb (Pickup et al., 2002) Rac1 (Fanto et al., 2000) raw (Byars et al., 1999) rl (Kockel et al., 1997) JraJbz.Scer\UAS puc (Sanyal et al., 2002) JraS82A.T92A.T107A.hs.sev Ras85D (Peverali et al., 1996) rl (Peverali et al., 1996) JraS82D.T92D.T107D.hs.sev phl (Peverali et al., 1996) JraScer\UAS.cEa kay (Sanyal et al., 2002) unspecified CrebB (Sanyal et al., 2002) dnc (Sanyal et al., 2002) Dok (Biswas et al., 2006) dpp (Staehling-Hampton et al., 1995) fz (Weber et al., 2000) hep (Miotto et al., 2006) L (Singh et al., 2006) Mnn1 (Cerrato et al., 2006) Ret (Read et al., 2005)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 61885 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0001291 DroID - A comprehensive database of gene and protein interactions. • FBgn0001291 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 36057 SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • P18289
Orthologs
OrthoDB Orthologs (52) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6PG6M6 Dipteran inclusive ortholog search EOG6894WK Insect inclusive ortholog search EOG6VQ8KD Arthropod inclusive ortholog search EOG6K3JCB Metazoa inclusive ortholog search EOG69WF8T
Orthologs in Drosophila Species (EOG6PG6M6)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Jra     Drosophila simulans   Dsim\GD15385 Y   Drosophila sechellia   Dsec\GM21184 Y   Drosophila erecta   Dere\GG24138 Y   Drosophila yakuba   Dyak\GE19337 Y   Drosophila ananassae   Dana\GF12018 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA30492 Y   Drosophila persimilis   Dper\GL17608 Y   Drosophila willistoni   Dwil\GK21594 Y   Drosophila virilis   Dvir\GJ22384 Y   Drosophila mojavensis   Dmoj\GI20534 Y   Drosophila grimshawi   Dgri\GH21247 Y
Orthologs in non-Drosophila Dipterans (EOG6894WK)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL003505   Anopheles gambiae Malaria mosquito  Agam\AGAP006386   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ000084
Orthologs in non-Dipteran Insects (EOG6VQ8KD)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB53318   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG014068   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH19161   Atta cephalotes Leafcutter ant  Acep\ACEP18115   Camponotus floridanus Florida carpenter ant  Cflo\CFLO13471   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL22826   Linepithema humile Argentine ant  Lhum\LH17191   Pogonomyrmex barbatus Red harvester ant  Pbar\PB21081   Solenopsis invicta Red fire ant  Sinv\SINV15314   Acyrthosiphon pisum Pea aphid  Apim\ACYPI003844   Acyrthosiphon pisum Pea aphid  Apim\ACYPI002386   Acyrthosiphon pisum Pea aphid  Apim\ACYPI001650   Bombyx mori Silkmoth  Bmor\BGIBMGA004164   Pediculus humanus Human body louse  Phum\PHUM379500   Tribolium castaneum Red flour beetle  Tcas\Jra
Orthologs in non-Insect Arthropods (EOG6K3JCB)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\JGI_V11_306002   Ixodes scapularis Deer tick  Isca\ISCW000545
Orthologs in non-Arthropod Metazoa (EOG69WF8T)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\jun-1   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_003102gn   Danio rerio Zebrafish  Drer\jund   Danio rerio Zebrafish  Drer\ENSDARG00000088371   Danio rerio Zebrafish  Drer\jun   Danio rerio Zebrafish  Drer\zgc:153924   Danio rerio Zebrafish  Drer\ENSDARG00000074378   Xenopus tropicalis Western clawed frog  Xtro\jun   Xenopus tropicalis Western clawed frog  Xtro\junb   Xenopus tropicalis Western clawed frog  Xtro\jund   Xenopus tropicalis Western clawed frog  Xtro\jund.2   Gallus gallus Domestic chicken  Ggal\JUN   Mus musculus House mouse  Mmus\Junb   Mus musculus House mouse  Mmus\Jund   Mus musculus House mouse  Mmus\Jun   Rattus norvegicus Norway rat  Rnor\Jun   Rattus norvegicus Norway rat  Rnor\ENSRNOG00000042838   Homo sapiens Human  Hsap\JUNB   Homo sapiens Human  Hsap\JUND   Homo sapiens Human  Hsap\JUN
Human Orthologs (3)
Gene OMIM HGNC Hsap\JUNB     Hsap\JUND     Hsap\JUN
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD15385 Drosophila sechellia Dsec\GM21184 Drosophila erecta Dere\GG24138 Drosophila yakuba Dyak\GE19337 Drosophila ananassae Dana\GF12018 Drosophila pseudoobscura pseudoobscura Dpse\GA30492 Drosophila persimilis Dper\GL17608 Drosophila willistoni Dwil\GK21594 Drosophila virilis Dvir\GJ22384 Drosophila mojavensis Dmoj\GI20534 Drosophila grimshawi Dgri\GH21247
Stocks & Reagents
Stocks Listed in FlyBase ( 7 )
Bloomington	7216 y1 w1118; P{UAS-Jra}2 31595 y1 v1; P{TRiP.JF01184}attP2 3273 cn1 JraIA109 bw1 sp1/CyO 9880 Adhfn23 pr1 cn1 Jra76-19/CyO
Kyoto	106951 cn1 JraIA109 bw1 sp1/CyO
VDRC	v107997 P{KK100582}VIE-260B v10835 w1118 P{GD4400}v10835
Genomic Clones ( 2 )
	BACR06J01 BACR09J12 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 40 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD25202
Other clones	DPiM_LD25202
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT04509 AT09868 AT10175 GH26769 GH28626 GM17859 RE11781 RE12112 RE24535 RE26731 RE26733 RH50828 SD16936 SD20220 SD26172
Other clones	38890 69939 70135 97316 190261 475319 525008 527892 528154 572956 CK01.72A.F9 Dmel_neb_004774_0619_0859 EC056139 EK057221 EK197422 EK197556 EK219338 EN00659 EN06339 EN08208 EN08640 EN17560 FGM317C02
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0001291 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 36057
Antibody Information
	polyclonal (FlyBase, 1992-)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: Jra l(2)46Ef (Goldstein et al., 2001)
Additional comments
Other Comments
	DNA-protein interactions: genome-wide binding profile assayed for Jra protein in Kc167 cells; see Chromatin_types_NKI collection report. Individual protein-binding experiments listed under "Samples" at GEO_GSE22069 (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE22069). (Filion et al., 2010) S2 cells treated with dsRNA generated against this gene show reduced phagocytosis of Candida albicans compared to untreated cells. (Stroschein-Stevenson et al., 2006) dsRNA made from templates generated with primers directed against this gene results in dorsal-closure defects in 79.4% of injected embryos. (Sano et al., 2005) dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003) Clonal analysis reveals that loss of function mutants cause defects in R3 induction and planar polarity determination, whereas gain of function mutants induce the R3 fate and associated polarity phenotypes. (Weber et al., 2000) Jra acts downstream of fz/dsh signaling in R3 specification and planar polarity determination. Jra is one of the transcription factors that mediates the effects of fz in planar polarity generation. (Weber et al., 2000) In vitro protein binding assays demonstrate kay is a poor candidate for a transcriptional activator acting through the Ubx cAMP response element (CRE). (Eresh et al., 1997) Jra in the embryo is a downstream target of the bsk signal transduction pathway during dorsal closure formation. The function of the bsk/Jra pathway is to control the localised expression of dpp. Both in the embryo and during photoreceptor cell determination Jra is not regulated by a pathway that involved rl. Jra is required for the initiation of dorsal closure (controls the organisation of the leading edge cells), but not for embryonic segmentation or photoreceptor cell differentiation in the eye. (Hou et al., 1997) Transcription factors Jra and aop are required for dorsal closure. Results suggest that the bsk pathway governs dorsal closure at least partially by regulating dpp expression via phosphorylation of Jra and aop. Jra function is not required for the specification of cell fate in the eye. (Riesgo-Escovar and Hafen, 1997) Jra and pntP2 do not cooperate during midline glia development. (Scholz et al., 1997, Scholz et al., 1997) Jra is a substrate for rl, part of the receptor tyrosine kinase signal transduction pathway which triggers photoreceptor differentiation during eye development. Mutant analysis implicates Jra phosphorylation in the choice between neuronal and non-neuronal fate during eye development. (Peverali et al., 1996) The DebA upstream region contains a binding site for the Jra/kay (AP-1) protein complex, which negatively regulates its transcription. (Goldstein, 1995) Jra cooperates with the ETS domain pnt gene product to act on common target genes which induce photoreceptor R7 fate in the developing eye. phyl might be one of the target genes. (Treier et al., 1995) Jra is required downstream of the sev signalling pathway for development in the eye. (Bohmann et al., 1994) Promoter deletion analysis of Jra-Ecol\CAT fusion constructs indicates that multiple cis-acting elements in the promoter region and the 5' non-coding region of the transcription unit control Jra expression. (Wang and Goldstein, 1994) The potential interplay of Jra with kay may play an important role in cell-type specific transcription during embryonic development. (Perkins et al., 1990) The AP-1 complex encodes two proteins that have functional and structural properties in common with mammalian Fos and Jun proto-oncogene products; kay and Jra, respectively. The biochemical properties of the Jra gene product have been examined in vitro and the expression pattern in developing embryos studied. (Perkins et al., 1990) Isolated from a genomic library using a human jun cDNA as a probe. (Zhang et al., 1990) Jra has been cloned, sequenced, and its expression pattern analysed. (Zhang et al., 1990)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_057238 NM_165739 NM_001273915 RefSeq (Proteins) • NP_476586 NP_724882 NP_001260844 DDBJ / EMBL / GenBank • AA941201 AAA28650 AAF58845 AAL13791 AAM71059 AC005469 AC005974 AGB93377 AW942721 AY058562 CAA38083 CAA73154 L33044 M36181 X54144 Y12573 Entrez Gene - A searchable database of RefSeq genes. • 36057 UniProtKB/Swiss-Prot • P18289
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Transcription factor Jun (IPR002112) Basic-leucine zipper domain (IPR004827) Jun-like transcription factor (IPR005643) Eukaryotic transcription factor, Skn-1-like, DNA-binding (IPR008917) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • LD25202
Linkouts
	BioGRID - A database of protein and genetic interactions • 61885 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0001291 DroID - A comprehensive database of gene and protein interactions. • FBgn0001291 DRSC - Results from RNAi screens. • FBgn0001291 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0001291 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG2275-RB FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0001291 FlyReactome - A curated knowledgebase of Drosophila melanogaster pathways • 85088 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 36057 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /gene/djun.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 36057 modMine - Data generated by the modENCODE project. • FBgn0001291 REDfly - A database of transcriptional regulatory elements. • FBgn0001291 SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • P18289
Synonyms & Secondary IDs ( 38 )
Reported As
Symbol Synonym	AP-1 (Harden, 2005, Stramer and Martin, 2005, Short et al., 2005, Zhang et al., 2002, Monroe et al., 2006, Bhaskara et al., 2006, Wang et al., 2007, Etter et al., 2005, Franciscovich et al., 2008) AP1 (Sanyal et al., 2003, Moussian and Uv, 2005, Sanyal et al., 2003, Mrinal and Nagaraju, 2010) CG2275 (De Gregorio et al., 2001, Schmidt et al., 2012, Bossuyt et al., 2009, Carrera et al., 2008, Keleman et al., 2009.8.5, Ni et al., 2008) c-Jun (Ciccarelli et al., 2003, Wang and Chang, 2003, Muralikrishna and Parnaik, 2001) cJun (Stronach and Perrimon, 2002) dAP-1 (Wang and Goldstein, 1993, Kim et al., 2007) dJRA (Briata et al., 1993, Perkins et al., 1988) d-JRA (Woodgett, 1991) dJra (Perkins et al., 1990, ) Djun (Miotto et al., 2006, Chen et al., 2002, Nedelcheva et al., 2001, French et al., 2003, Van Aelst and Symons, 2002, Strutt et al., 2002, Hudson and Goldstein, 2002, Suzanne et al., 2001, Botella et al., 2001, Guru et al., 2001, Fanto, 2000, Fanto et al., 2000, Cox et al., 2000, McEwen et al., 2000, Szuts and Bienz, 2000, Wang, 1994, Noselli and Agnes, 1999, Kyriakis, 1999, Goldstein et al., 1999, Agnes and Noselli, 1999, Goberdhan and Wilson, 1998, Su et al., 1998, Goldstein et al., 1998, Martin-Blanco, 1997, Hou et al., 1997, Hou et al., 1997, Wang and Goldstein, 1994, Wang and Goldstein, 1993, Zhang et al., 1990, Nazario-Yepiz and Riesgo-Escovar, 2007, Lehmann et al., 2002, Bossuyt et al., 2009, Cobreros-Reguera et al., 2010, Weber et al., 2012) DJun (Hoeffer et al., 2003, Varfolomeev and Ashkenazi, 2004, Strutt, 2003, Lockwood and Bodmer, 2002, Dobens et al., 2001, Rorth et al., 2000, Kumar and Moses, 2000, Roche, 2000, Tateno et al., 2000, Stronach and Perrimon, 1999, Dequier et al., 1999, Ricos et al., 1999, Noselli et al., 1998, Han et al., 1998, Ip and Davis, 1998, Noselli, 1998, Anonymous, 1997, Riesgo-Escovar and Hafen, 1997, Riesgo-Escovar and Hafen, 1997, Riesgo-Escovar and Hafen, 1997, Sluss et al., 1996, Dominguez and Hafen, 1996, Singh et al., 2006, Mikhaylova and Nurminsky, 2011, Lee et al., 2007, Guichard et al., 2006, Campos et al., 2010) DJUN (Xia and Karin, 2004, Reed et al., 2001, Weitzman, 2000, Chen et al., 2000, Anonymous, 1998, Sluss and Davis, 1997) D-jun (Kockel et al., 2001, Yamada et al., 2003, Cerrato et al., 2002, Mihaly et al., 2001, Raabe, 2000, Granderath and Klambt, 1999, Isaksson et al., 1997, Scholz et al., 1997, Scholz et al., 1997, Dickson, 1995, Sekyrova et al., 2006, Collins et al., 2006, Bhaskara et al., 2008) D-Jun (Jindra et al., 2004, Ciapponi and Bohmann, 2002, Ciapponi and Bohmann, 2002, Dobens and Raftery, 2001, Blau and Young, 1999, Zeitlinger and Bohmann, 1999, Sutherland and Witke, 1999, Zeitlinger et al., 1998, Knust, 1997, Riese et al., 1997, Eresh et al., 1997, Peverali et al., 1996, Isaksson et al., 1996, Jindra et al., 2005, Collins et al., 2006, Liu et al., 2009, Balakireva et al., 2006) d-Jun (Chang et al., 2003, De Gregorio et al., 2002, De Gregorio et al., 2001) d-jun (Jindra et al., 2003) dJun (Riesgo-Escovar et al., 1997, Delaney et al., 2006, Sano et al., 2005, Bond and Foley, 2009, Bakal et al., 2008, Franciscovich et al., 2008, Buchon et al., 2009, Tokusumi et al., 2009, Xi et al., 2008, Pereira et al., 2011) dJUN (Treisman, 1996, Corl et al., 2009, Haghayeghi et al., 2010) djun (Wu et al., 2008, Mozer and Sandstrom, 2012) D-jun/Jra (Friedman and Perrimon, 2006) dm-Jun (Bohmann et al., 1994) Jra (Bates et al., 2008, Moorman et al., 2006, Moorman et al., 2006, Christensen and Cook, 2007.5.8, Stroschein-Stevenson et al., 2006, Zhao et al., 2008, Mace et al., 2005, Lecuyer et al., 2007, Grimberg et al., 2011, Jungreis et al., 2011, Sandmann et al., 2006, Tadros et al., 2007, Kim et al., 2007, Stielow et al., 2008, Weake et al., 2011, Bossuyt et al., 2009, Carrera et al., 2008, Tokusumi et al., 2009, Kim et al., 2005, de Wit et al., 2008, Campos et al., 2010, Xi et al., 2008, Curtis et al., 2007, Suganuma et al., 2010, Bossing et al., 2012, Lee et al., 2008, Tokusumi et al., 2012, Ling et al., 2012, Jungreis et al., 2011, Japanese National Institute of Genetics, 2012.5.21, Lim et al., 2012, Chakrabarti et al., 2012, Spedale et al., 2012, Weber et al., 2012) JRA (Collier et al., 2004, Greil et al., 2007, van Steensel et al., 2010, Filion et al., 2010) jra (Goldstein et al., 2001, Neuman-Silberberg, 2007, Davis et al., 2008, Yi et al., 2007, Mrinal and Nagaraju, 2010, Jemc et al., 2012) jun (Munoz-Descalzo et al., 2005, Cerrato et al., 2004, Jenny et al., 2003, Igaki et al., 2002, Adler, 2002, Settleman, 2001, Sanyal et al., 2002, Pickup et al., 2002, Ciapponi and Bohmann, 2002, Chandrasekharappa et al., 2001, Fernandez et al., 2000, Stronach and Perrimon, 2000, Potter et al., 2000, Paricio et al., 1999, Lamka and Lipshitz, 1999, Boutros et al., 1998, Kockel et al., 1998, Scholz et al., 1997, Kockel et al., 1997, Scholz et al., 1997, Treier et al., 1995, Goldstein, 1995, Begemann et al., 1995, Simpson et al., 1992, Tyler et al., 2007, Davis et al., 2008, Cerrato et al., 2006, Polaski et al., 2006, Mathieu et al., 2007, Schimmelpfeng et al., 2006, Fanto and McNeill, 2004, Muñoz-Descalzo et al., 2007, Homsy et al., 2006, Massaro et al., 2009, Milton et al., 2011, Melani et al., 2008, Hartwig et al., 2008, Pearson et al., 2009, Weber et al., 2008, Haussmann et al., 2008, Franciscovich et al., 2008, Vonhoff et al., 2013) Jun (Leyssen et al., 2005, Udomsinprasert et al., 2004, Veeman et al., 2003, Tran and Berg, 2003, Miotto et al., 2004, Chen et al., 2002, Boutros et al., 2002, Jacinto et al., 2002, Jacinto et al., 2001, Tree et al., 2002, Mlodzik, 2002, Sanyal et al., 2003, Schaeffer et al., 2002, Miotto et al., 2002, Weber et al., 2000, Kockel, 1997.4.15, Zeitlinger et al., 1997, Eaton, 1997, Blaumueller and Mlodzik, 1997, Wassarman et al., 1995, Mlodzik et al., 1993, Mace et al., 2005, Collins et al., 2006, Rynes et al., 2012, Wu et al., 2008, Llense and Martín-Blanco, 2008, Shevelyov et al., 2009, Kim et al., 2009, Rallis et al., 2010, Sanyal et al., 2003, Etter et al., 2005, Sekyrova et al., 2010, Xiong et al., 2010, Bangi et al., 2012) JUN (Tsichritzis et al., 2007, Yavari et al., 2010) l(2)46Ef   l(2)IA109 (Staehling-Hampton et al., 1995, Tearle and Nusslein-Volhard, 1987, ) l(2R)IA109 (Riesgo-Escovar and Hafen, 1997) V (Carney et al., 1997)
Name Synonym	activator protein 1 (Bhaskara et al., 2006) Complementation group V (Goldstein et al., 2001, Carney et al., 1997) Jun oncogene (Goldstein et al., 1993) Jun-related antigen (Moorman et al., 2006, Moorman et al., 2006, Zhao et al., 2008, Tadros et al., 2007, Zhan et al., 2007, Mrinal and Nagaraju, 2010, Jemc et al., 2012) Jun related antigen (Campos et al., 2010) lethal(2)1A109 (Staehling-Hampton et al., 1995) lethal(2)IA109
Secondary FlyBase IDs
	FBgn0002124 FBgn0015487 FBgn0017591 FBgn0023037
References ( 308 )
Generate a list of	All references relating to this gene ( 308 )
List References by type	Research paper  ( 190 ) Supplementary material  ( 10 ) Review  ( 47 ) Erratum  ( 2 ) Personal communication to FlyBase  ( 8 ) Abstract  ( 40 ) FlyBase analysis  ( 2 ) Letter  ( 1 ) DNA/RNA sequence record  ( 2 ) Stock list  ( 2 ) Protein sequence record  ( 1 ) Website  ( 1 ) Curated genome annotation  ( 2 )
Recent research papers ( 21 )
	Vonhoff et al., 2013, Development 140(3): 606--616 Temporal coherency between receptor expression, neural activity and AP-1-dependent transcription regulates Drosophila motoneuron dendrite development. [FBrf0220342] Bangi et al., 2012, EMBO Rep. 13(6): 569--576 Immune response to bacteria induces dissemination of Ras-activated Drosophila hindgut cells. [FBrf0218473] Bossing et al., 2012, Dev. Cell 23(2): 433--440 Disruption of Microtubule Integrity Initiates Mitosis during CNS Repair. [FBrf0219196] Brock et al., 2012, J. Cell Sci. 125(23): 5667--5676 Transcriptional regulation of Profilin during wound closure in Drosophila larvae. [FBrf0220862] Chakrabarti et al., 2012, Cell Host Microbe 12(1): 60--70 Infection-induced host translational blockage inhibits immune responses and epithelial renewal in the Drosophila gut. [FBrf0218984] Chatterjee and Bohmann, 2012, PLoS ONE 7(4): e34063 A Versatile ΦC31 Based Reporter System for Measuring AP-1 and Nrf2 Signaling in Drosophila and in Tissue Culture. [FBrf0218067] Jemc et al., 2012, Dev. Biol. 367(2): 114--125 raw Functions through JNK signaling and cadherin-based adhesion to regulate Drosophila gonad morphogenesis. [FBrf0218557] Lim et al., 2012, PLoS ONE 7(5): e37028 Ebi/AP-1 Suppresses Pro-Apoptotic Genes Expression and Permits Long-Term Survival of Drosophila Sensory Neurons. [FBrf0218464] Ling et al., 2012, J. Neurosci. 32(47): 16959--16970 KAYAK-α Modulates Circadian Transcriptional Feedback Loops in Drosophila Pacemaker Neurons. [FBrf0219997] Mozer and Sandstrom, 2012, Mol. Cell. Neurosci. 51(3-4): 89--100 Drosophila neuroligin 1 regulates synaptic growth and function in response to activity and phosphoinositide-3-kinase. [FBrf0219839] Rynes et al., 2012, Mol. Cell. Biol. 32(19): 3949--3962 Activating transcription factor 3 regulates immune and metabolic homeostasis. [FBrf0219385] Schmidt et al., 2012, J. Neurosci. 32(22): 7466--7476 Kinesin heavy chain function in Drosophila glial cells controls neuronal activity. [FBrf0218491] Tokusumi et al., 2012, PLoS ONE 7(7): e41604 Gene regulatory networks controlling hematopoietic progenitor niche cell production and differentiation in the Drosophila lymph gland. [FBrf0219204] Weber et al., 2012, Genetics 191(1): 145--162 Novel regulators of planar cell polarity: a genetic analysis in Drosophila. [FBrf0218210] Grimberg et al., 2011, Mol. Cell. Biol. 31(4): 897--909 Basic Leucine Zipper Protein Cnc-C Is a Substrate and Transcriptional Regulator of the Drosophila 26S Proteasome. [FBrf0212906] Horn et al., 2011, Nat. Methods 8(4): 341--346 Mapping of signaling networks through synthetic genetic interaction analysis by RNAi. [FBrf0213352] Jungreis et al., 2011, Genome Res. 21(12): 2096--2113 Evidence of abundant stop codon readthrough in Drosophila and other metazoa. [FBrf0216845] Mikhaylova and Nurminsky, 2011, BMC Biol. 9: 29 Lack of global meiotic sex chromosome inactivation, and paucity of tissue-specific gene expression on the Drosophila X chromosome. [FBrf0213839] Milton et al., 2011, Proc. Natl. Acad. Sci. U.S.A. 108(42): 17521--17526 Oxidative stress induces overgrowth of the Drosophila neuromuscular junction. [FBrf0216440] Pereira et al., 2011, PLoS ONE 6(12): e26182 Integrin-Dependent Activation of the JNK Signaling Pathway by Mechanical Stress. [FBrf0216993] Weake et al., 2011, Genes Dev. 25(14): 1499--1509 Post-transcription initiation function of the ubiquitous SAGA complex in tissue-specific gene activation. [FBrf0214372] Show all research papers ...
Recent reviews ( 2 )
	Spedale et al., 2012, Genes Dev. 26(6): 527--541 ATAC-king the complexity of SAGA during evolution. [FBrf0219841] Broadie et al., 2011, Dev. Neurobiol. 71(11): 1102--1130 Extracellular matrix and its receptors in drosophila neural development. [FBrf0216377] Show all reviews ...