FlyBase Gene Report: Dmel\kel

General Information

Symbol

Dmel\kel

Species

D. melanogaster

Name

kelch

Annotation Symbol

CG7210

Feature Type

protein_coding_gene

FlyBase ID

FBgn0001301

Gene Model Status

Current

Stock Availability

10 publicly available

Gene Summary

kelch (kel) encodes a substrate targeting component of a Cullin3-RING ubiquitin E3 ligase. It binds F-actin in vitro, while its predominant in vivo function is in the ubiquitin-proteasome pathway. It has an essential role in oogenesis, where it is required for cytoskeletal organization in ovarian ring canals. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
UniProtKB
Red Book
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

36E5-36E6

Sequence location

2L:18,129,884..18,138,083 [-]

Recombination map

2-53

RefSeq locus

NT_033779 REGION:18129884..18138083

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (12 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables actin binding

inferred from direct assay

(Kelso et al., 2002)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (8 terms)

Terms Based on Experimental Evidence (8 terms)

CV Term

Evidence

References

involved_in actin cytoskeleton organization

inferred from mutant phenotype

(Djagaeva et al., 2005)

involved_in cellularization

inferred from mutant phenotype

(Pilot et al., 2006)

involved_in female germline ring canal formation

inferred from mutant phenotype

(Robinson and Cooley, 1997)

inferred from direct assay

(Robinson et al., 1994)

involved_in oocyte karyosome formation

inferred from mutant phenotype

(Djagaeva et al., 2005)

involved_in oogenesis

inferred from mutant phenotype

(Djagaeva et al., 2005)

inferred from high throughput mutant phenotype

(Schupbach and Wieschaus, 1991)

involved_in ovarian follicle cell migration

inferred from high throughput mutant phenotype

(Schupbach and Wieschaus, 1991)

involved_in ovarian fusome organization

inferred from mutant phenotype

(Djagaeva et al., 2005)

involved_in ovarian nurse cell to oocyte transport

inferred from mutant phenotype

(Djagaeva et al., 2005, Robinson and Cooley, 1997)

Terms Based on Predictions or Assertions (0 terms)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

located_in female germline ring canal

inferred from direct assay

(Robinson and Cooley, 1997)

located_in germline ring canal

inferred from direct assay

(Kelso et al., 2002, Robinson and Cooley, 1997)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

located_in female germline ring canal inner rim

traceable author statement

(Sokol and Cooley, 1999, Robinson et al., 1997)

located_in germline ring canal

traceable author statement

(Jackson and Berg, 1999)

Gene Group (FlyBase)

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Protein Function (UniProtKB)

Component of ring canals that regulates the flow of cytoplasm between cells. May be involved in the regulation of cytoplasm flow from nurse cells to the oocyte during oogenesis. Binds actin.

(UniProt, Q04652)

Phenotypic Description (Red Book; Lindsley and Zimm 1992)

kel: kelch

Recessive female sterile. Nurse cell cytoplasm not incorporated in oocyte. Small eggs "open" at anterior pole since egg membranes are not deposited around whole oocyte.

Summary (Interactive Fly)

cytoskeletal protein - actin cross-linker - during oogenesis, phosphorylation of Kelch is critical for the proper morphogenesis of actin during ring canal growth - actin cytoskeletal organization in Drosophila germline ring canals depends on Kelch function in a Cullin-RING E3 ligase.

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\kel for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure New Section

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q04652)

If you don't see the viewer to the right, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Comments on Gene Model

Stop-codon suppression (UGA) postulated; FBrf0057892.

Stop-codon suppression supported by data in FBrf0216884.

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.51

Sequence Ontology: Class of Gene

gene_with_stop_codon_read_through

(Jungreis et al., 2011, Xue and Cooley, 1993)

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

kel-RA

FBtr0081042

NM_165242

5476

689

kel-RB

FBtr0081043

NM_057241

5476

1477

Additional Transcript Data and Comments

Reported size (kB)

6.0 (northern blot)

(Xue and Cooley, 1993)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

kel-PA

FBpp0080595

76.6

689

5.40

NP_724095

AAF53651

kel-PB

FBpp0080596

160.0

1477

6.46

NP_476589

AAN11182

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

160, 76 (kD observed)

(Robinson and Cooley, 1997)

787, 688 (aa); 83.5, 76.5 (kD)

(Xue and Cooley, 1993)

Comments

The 787aa kel protein is the second protein predicted from the bicistronic kel transcript. It has not been physically detected.

(Xue and Cooley, 1993)

One of a couple of protein products generated from a bicistronic transcript.

(FlyBase, 1992-)

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\kel using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

kel_1

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

No Assay Recorded

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage | male

(Xue and Cooley, 1993)

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

central brain anlage in statu nascendi

(Fisher et al., 2012)

dorsal ectoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)

antennal anlage in statu nascendi

Comment: reported as procephalic ectoderm anlage in statu nascendi

(Fisher et al., 2012)

dorsal head epidermis anlage in statu nascendi

Comment: reported as procephalic ectoderm anlage in statu nascendi

(Fisher et al., 2012)

visual anlage in statu nascendi

Comment: reported as procephalic ectoderm anlage in statu nascendi

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | faint | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | faint | ubiquitous

(Fisher et al., 2012)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage | female

(Xue and Cooley, 1993)

oogenesis & adult stage | female

ovary

(Xue and Cooley, 1993)

Additional Descriptive Data

The kel transcript is most abundant in ovaries but is also detected in males and ovarectomized females.

(Xue and Cooley, 1993)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

No Assay Recorded

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage & oogenesis

egg chamber

(Xue and Cooley, 1993)

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage & oogenesis

ring canal

(Xue and Cooley, 1993)

oogenesis & adult stage | female

germarium region 3 & nurse cell ring canal

(Robinson et al., 1994)

adult stage oogenesis stage S2 -- S14

nurse cell ring canal

(Robinson et al., 1994)

western blot

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

imaginal disc

(Robinson and Cooley, 1997)

testis

(Robinson and Cooley, 1997)

pupal stage

(Robinson and Cooley, 1997)

adult stage

(Robinson and Cooley, 1997)

adult stage & oogenesis

ovary

(Robinson and Cooley, 1997)

Additional Descriptive Data

kel protein is observed in ring canals starting in germarium region 3. Initially staining is seen in a subset of ring canals. All ring canals are stained by stage 3.

(Robinson et al., 1994)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in female germline ring canal

inferred from direct assay

(Robinson and Cooley, 1997)

located_in germline ring canal

inferred from direct assay

(Kelso et al., 2002, Robinson and Cooley, 1997)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\kel in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\kel

View exonic expression by tissue for Dmel\kel

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0001301

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

35084/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0001301

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0001301

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0001301

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG7210

Flygut - An atlas of the Drosophila adult midgut

FBgn0001301

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\kel

Stages(s) 1-3
Stages(s) 4-6
Stages(s) 7-8
Stages(s) 9-10

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 15 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 36 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of kel

Transgenic constructs containing regulatory region of kel

Aberrations (Deficiencies and Duplications) ( 18 )

Inferred from experimentation ( 18 )

Gene not disrupted in

Df(2L)A33

(Hardy, 2005.6.1)

Df(2L)M36F18

(Steward and Nusslein-Volhard, 1986)

Df(2L)TW119

(Steward and Nusslein-Volhard, 1986)

Df(2L)TW202

(Steward and Nusslein-Volhard, 1986)

Inferred from location ( 0 )

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Sterility

Allele

female fertile, with Scer\GAL4^{VP16.mat.αTub67C}

kel^UASp.cHa

female sterile

female sterile, with Scer\GAL4^{VP16.mat.αTub67C}

female sterile | recessive

Other Phenotypes

Allele

abnormal cell shape

kel^DE1

abnormal cell size | adult stage | female

kel^DE1

abnormal cell size | adult stage | female, with Scer\GAL4^{VP16.mat.αTub67C}

kel^FE.UASp

decreased cell size | oogenesis

kel^DE1

increased cell death

kel^DE1

wild-type

Phenotype manifest in

Allele

actin cytoskeleton | adult stage | female

kel^DE1

actin cytoskeleton | adult stage | female, with Scer\GAL4^{VP16.mat.αTub67C}

kel^FE.UASp

actin filament

kel^DE1

actin filament & nurse cell ring canal

kel^Y627A.otu

egg

egg chamber

filamentous actin

kel^DE1

filamentous actin | oogenesis | oogenesis

kel^DE1

inner nurse cell ring canal rim

kel^{ORF1-R.otu.Tag:MYC}

karyosome

kel^DE1

nurse cell

nurse cell & actin filament

kel^DE1

nurse cell & nucleus

kel^DE1

nurse cell & plasma membrane

kel^{ΔNORF1.otu.Tag:MYC}

nurse cell fusome

kel^DE1

nurse cell ring canal

nurse cell ring canal, with Scer\GAL4^{VP16.mat.αTub67C}

nurse cell ring canal | oogenesis stage S6

kel^DE1

nurse cell ring canal | oogenesis stage S7

kel^DE1

nurse cell ring canal | oogenesis stage S8

kel^DE1

nurse cell ring canal | oogenesis stage S9

kel^DE1

nurse cell ring canal | oogenesis stage S10

kel^DE1

nurse cell ring canal | oogenesis stage S11

kel^DE1

nurse cell ring canal | oogenesis stage S12

kel^DE1

nurse cell ring canal | oogenesis stage S13

kel^DE1

nurse cell ring canal | oogenesis stage S14

kel^DE1

nurse cell ring canal rim

kel^DE1

oocyte

oocyte, with Scer\GAL4^{VP16.mat.αTub67C}

kel^FE.UASp

oocyte & actin filament

kel^DE1

stalk follicle cell

kel^DE1

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (13)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\KLHL3

10 of 15

Yes

Hsap\KLHL2

9 of 15

Yes

Hsap\CCIN

1 of 15

Yes

Hsap\GAN

1 of 15

Yes

Hsap\KBTBD2

1 of 15

Yes

Hsap\KBTBD6

1 of 15

Yes

Hsap\KBTBD7

1 of 15

Yes

Hsap\KBTBD8

1 of 15

Yes

Hsap\KLHL11

1 of 15

Yes

Hsap\KLHL12

1 of 15

Yes

Hsap\KLHL38

1 of 15

Yes

Hsap\KLHL40

1 of 15

Yes

Hsap\KLHL41

1 of 15

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (13)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Klhl3

10 of 15

Yes

Mmus\Klhl2

9 of 15

Yes

Mmus\Ccin

1 of 15

Yes

Mmus\Gan

1 of 15

Yes

Mmus\Kbtbd2

1 of 15

Yes

Mmus\Kbtbd6

1 of 15

Yes

Mmus\Kbtbd7

1 of 15

Yes

Mmus\Kbtbd8

1 of 15

Yes

Mmus\Klhl11

1 of 15

Yes

Mmus\Klhl12

1 of 15

Yes

Mmus\Klhl38

1 of 15

Yes

Mmus\Klhl40

1 of 15

Yes

Mmus\Klhl41

1 of 15

Yes

Rattus norvegicus (Norway rat) (12)

Rnor\Klhl2

8 of 13

Yes

Rnor\Klhl3

3 of 13

Yes

Rnor\Ccin

1 of 13

Yes

Rnor\Gan

1 of 13

Yes

Rnor\Kbtbd2

1 of 13

Yes

Rnor\Kbtbd6

1 of 13

Yes

Rnor\Kbtbd8

1 of 13

Yes

Rnor\Klhl11

1 of 13

Yes

Rnor\Klhl12

1 of 13

Yes

Rnor\Klhl38

1 of 13

Yes

Rnor\Klhl40

1 of 13

Yes

Rnor\Klhl41

1 of 13

Yes

Xenopus tropicalis (Western clawed frog) (2)

Xtro\gan

1 of 12

Yes

Xtro\klhl2

1 of 12

Yes

Danio rerio (Zebrafish) (15)

Drer\klhl3

10 of 15

Yes

Drer\klhl2

8 of 15

Yes

Drer\gan

1 of 15

Yes

Drer\kbtbd2

1 of 15

Yes

Drer\kbtbd4

1 of 15

Yes

Drer\kbtbd7

1 of 15

Yes

Drer\kbtbd8

1 of 15

Yes

Drer\klhl11

1 of 15

Yes

Drer\klhl38a

1 of 15

Yes

Drer\klhl38b

1 of 15

Yes

Drer\klhl40a

1 of 15

Yes

Drer\klhl40b

1 of 15

Yes

Drer\klhl41a

1 of 15

Yes

Drer\klhl41b

1 of 15

Yes

Drer\si:ch73-29c22.1

1 of 15

Caenorhabditis elegans (Nematode, roundworm) (5)

Cele\kel-1

11 of 15

Yes

Cele\F47D12.7

1 of 15

Cele\kel-8

1 of 15

Yes

Cele\kel-10

1 of 15

Cele\W07A12.4

1 of 15

Arabidopsis thaliana (thale-cress) (1)

Atha\AT5G01660

1 of 9

Yes

Saccharomyces cerevisiae (Brewer's yeast) (0)

No records found.

Schizosaccharomyces pombe (Fission yeast) (0)

No records found.

Other Organism Orthologs (via OrthoDB)

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (7)

Gene Symbol

Score

Source

Alignment

2 of 10

2 of 10

2 of 10

dbo

2 of 10

Keap1

2 of 10

klhl10

2 of 10

KLHL18

2 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

KLHL3; kelch like family member 3

10 of 15

605775

PSEUDOHYPOALDOSTERONISM, TYPE IID; PHA2D

KLHL2; kelch like family member 2

9 of 15

605774

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

kel

proximity-dependent biotin identification, Identification by mass spectrometry, western blot, anti tag coimmunoprecipitation

(Mannix et al., 2019, Hudson and Cooley, 2010)

kel - Atx2

proximity-dependent biotin identification, Identification by mass spectrometry

(Mannix et al., 2019)

kel - Brr2

tandem affinity purification, Identification by mass spectrometry, two hybrid

(Hudson et al., 2019)

kel - CG11417

proximity-dependent biotin identification, Identification by mass spectrometry

(Mannix et al., 2019)

kel - CG2199

proximity-dependent biotin identification, Identification by mass spectrometry

(Mannix et al., 2019)

kel - CG30122

proximity-dependent biotin identification, Identification by mass spectrometry

(Mannix et al., 2019)

kel - CG42232

proximity-dependent biotin identification, Identification by mass spectrometry

(Mannix et al., 2019)

kel - CG4847

proximity-dependent biotin identification, Identification by mass spectrometry

(Mannix et al., 2019)

kel - CG5126

proximity-dependent biotin identification, Identification by mass spectrometry

(Mannix et al., 2019)

kel - CG5787

proximity-dependent biotin identification, Identification by mass spectrometry

(Mannix et al., 2019)

kel - CG8545

proximity-dependent biotin identification, Identification by mass spectrometry

(Mannix et al., 2019)

kel - CG9336

proximity-dependent biotin identification, Identification by mass spectrometry

(Mannix et al., 2019)

kel - Capr

proximity-dependent biotin identification, Identification by mass spectrometry

(Mannix et al., 2019)

kel - Chro

proximity-dependent biotin identification, Identification by mass spectrometry

(Mannix et al., 2019)

kel - Ciz1

proximity-dependent biotin identification, Identification by mass spectrometry

(Mannix et al., 2019)

kel - Cpsf6

proximity-dependent biotin identification, Identification by mass spectrometry

(Mannix et al., 2019)

kel - Cul3

coimmunoprecipitation, anti tag western blot, anti tag coimmunoprecipitation, pull down, western blot

(Hudson et al., 2015, Strutt et al., 2013, Hudson and Cooley, 2010)

kel - Cyp6d4

proximity-dependent biotin identification, Identification by mass spectrometry

(Mannix et al., 2019)

kel - Elys

proximity-dependent biotin identification, Identification by mass spectrometry

(Mannix et al., 2019)

kel - Grip71

tandem affinity purification, Identification by mass spectrometry, two hybrid

(Hudson et al., 2019)

kel - Gs1

proximity-dependent biotin identification, Identification by mass spectrometry

(Mannix et al., 2019)

kel - GstD3

proximity-dependent biotin identification, Identification by mass spectrometry

(Mannix et al., 2019)

kel - Hrb27C

proximity-dependent biotin identification, Identification by mass spectrometry

(Mannix et al., 2019)

kel - Hrb98DE

proximity-dependent biotin identification, Identification by mass spectrometry

(Mannix et al., 2019)

kel - Ide

proximity-dependent biotin identification, Identification by mass spectrometry

(Mannix et al., 2019)

kel - Larp4B

proximity-dependent biotin identification, Identification by mass spectrometry

(Mannix et al., 2019)

kel - Msp300

proximity-dependent biotin identification, Identification by mass spectrometry

(Mannix et al., 2019)

kel - Ns3

proximity-dependent biotin identification, Identification by mass spectrometry

(Mannix et al., 2019)

kel - Pep

proximity-dependent biotin identification, Identification by mass spectrometry

(Mannix et al., 2019)

kel - Rbm13

proximity-dependent biotin identification, Identification by mass spectrometry

(Mannix et al., 2019)

kel - SF2

proximity-dependent biotin identification, Identification by mass spectrometry

(Mannix et al., 2019)

kel - Saf-B

proximity-dependent biotin identification, Identification by mass spectrometry

(Mannix et al., 2019)

kel - Sdb

proximity-dependent biotin identification, Identification by mass spectrometry

(Mannix et al., 2019)

kel - Spn43Ab

proximity-dependent biotin identification, Identification by mass spectrometry

(Mannix et al., 2019)

kel - Spt5

proximity-dependent biotin identification, Identification by mass spectrometry

(Mannix et al., 2019)

kel - Sumo

proximity-dependent biotin identification, Identification by mass spectrometry, proximity ligation assay, fluorescence microscopy

(Mannix et al., 2019)

kel - Top1

proximity-dependent biotin identification, Identification by mass spectrometry

(Mannix et al., 2019)

kel - Unc-115a

proximity-dependent biotin identification, Identification by mass spectrometry

(Mannix et al., 2019)

kel - btz

proximity-dependent biotin identification, Identification by mass spectrometry

(Mannix et al., 2019)

kel - cg

proximity-dependent biotin identification, Identification by mass spectrometry

(Mannix et al., 2019)

kel - cher

proximity-dependent biotin identification, Identification by mass spectrometry, western blot

(Mannix et al., 2019)

kel - cora

proximity-dependent biotin identification, Identification by mass spectrometry

(Mannix et al., 2019)

kel - dsh

anti tag coimmunoprecipitation, anti tag western blot

(Strutt et al., 2013)

kel - hts

proximity-dependent biotin identification, western blot, tandem affinity purification, Identification by mass spectrometry, proximity ligation assay, fluorescence microscopy

(Hudson et al., 2019, Mannix et al., 2019)

kel - lig

proximity-dependent biotin identification, Identification by mass spectrometry

(Mannix et al., 2019)

kel - mEFTs

proximity-dependent biotin identification, Identification by mass spectrometry

(Mannix et al., 2019)

kel - mei-38

two hybrid, tandem affinity purification, Identification by mass spectrometry

(Hudson et al., 2019)

kel - mod(mdg4)

proximity-dependent biotin identification, Identification by mass spectrometry

(Mannix et al., 2019)

kel - nito

proximity-dependent biotin identification, Identification by mass spectrometry

(Mannix et al., 2019)

kel - pyd

proximity-dependent biotin identification, Identification by mass spectrometry

(Mannix et al., 2019)

kel - pzg

proximity-dependent biotin identification, Identification by mass spectrometry

(Mannix et al., 2019)

kel - qkr58E-1

proximity-dependent biotin identification, Identification by mass spectrometry

(Mannix et al., 2019)

kel - rump

proximity-dependent biotin identification, Identification by mass spectrometry

(Mannix et al., 2019)

kel - smid

proximity-dependent biotin identification, Identification by mass spectrometry

(Mannix et al., 2019)

kel - stc

proximity-dependent biotin identification, Identification by mass spectrometry

(Mannix et al., 2019)

kel - yps

proximity-dependent biotin identification, Identification by mass spectrometry

(Mannix et al., 2019)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

kel

suppressible

hts

(Hudson et al., 2019)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

hts

suppressible

kel

(Gerdes et al., 2020)

hts|Prosβ5

enhanceable

kel

(Hudson et al., 2019)

Prosβ5

enhanceable

kel

(Hudson et al., 2015)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

61080

DroID - A comprehensive database of gene and protein interactions.

FBgn0001301

MIST (protein-protein) - An integrated Molecular Interaction Database

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0001301

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-53

Cytogenetic map

36E5-36E6

Sequence location

2L:18,129,884..18,138,083 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

36E5-36E6

Limits computationally determined from genome sequence between P{lacW}Aac11^k06710 and P{EP}CG10413^EP2164

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

36E-36F

(determined by in situ hybridisation)

(Spradling et al., 1999, Cooley et al., 1992)

36E1-36E3

(determined by in situ hybridisation)

(Xue and Cooley, 1993)

Experimentally Determined Recombination Data

Location

2-53

(FlyBase, 2016)

2-53.2

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (10)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

4893

w^*; kel^DE1 cn¹/CyO

31251

y¹ v¹; P{TRiP.JF01768}attP2

41484

y¹ w^*; Mi{MIC}kel^MI05229

13269

y¹ w^67c23; P{SUPor-P}kel^KG03947

60224

y¹ w^67c23; Mi{PT-GFSTF.2}kel^{MI05229-GFSTF.2}

77759

y¹ w^*; Mi{Trojan-GAL4.2}kel^{MI05229-TG4.2}/SM6a

55612

y¹ sc^* v¹ sev²¹; P{TRiP.HMC03751}attP40

107933

v330145

v105397

Genomic Clones (25)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (30)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

LD29455

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0001301

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0001301

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

35084

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Robinson and Cooley, 1997)

monoclonal

(Xue and Cooley, 1993)

Commercially Available Antibodies

Developmental Studies Hybridoma Bank - Monoclonal antibodies for use in research

kel_1B

Other Information

Relationship to Other Genes

Source for database identify of

Source for database merge of

Additional comments

Other Comments

Embryos injected with dsRNA made from templates generated with primers directed against this gene show a broad range of defects at the beginning of cellularisation, including falling of some nuclei from the cortex, and a reduction of nuclear elongation. They also show defects in membrane invagination during cellularisation.

(Pilot et al., 2006)

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

dsRNA made from templates generated with primers directed against this gene is tested in an RNAi screen for effects on actin-based lamella formation.

(Rogers et al., 2003)

kel protein is tyrosine phosphorylated in a Src64B-dependent pathway.

(Kelso et al., 2002)

kel is essential for the proper organization of actin in ovarian ring canals.

(Kelso et al., 2002)

The kel gene encodes two proteins, a 76kD open reading frame 1 (ORF1) protein and a 160kD full-length (ORF1 + ORF2) protein. Transgenic analysis suggests that the 76kD ORF1 protein is sufficient for kel function.

(Robinson and Cooley, 1997)

The amino-terminal region of the kel protein regulates the timing of kel protein localisation to the ring canals. The amino-terminal half of the kel protein including the BTB domain mediates dimerisation. The kelch repeat domain is necessary and sufficient for ring canal localisation.

(Robinson and Cooley, 1997)

The kel protein becomes localized to ring canals hours after hts and actin, and is necessary for maintaining a highly ordered ring canal rim.

(Robinson et al., 1994)

Mutations cause female sterility. Molecular and expression analysis suggests that kelch produces a component of ring canals that regulates the flow of cytoplasm between cells.

(Xue and Cooley, 1993)

Mutations at the kel locus cause defects in midoogenesis.

(Schupbach and Wieschaus, 1991)

Origin and Etymology

Discoverer

Schupbach.

Etymology

"kelch" is German for "goblet", reflecting the shape of eggs formed by mutant females.

(Xue and Cooley, 1993)

Identification

External Crossreferences and Linkouts ( 55 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

35084

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q04652

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q04652

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q04652

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0001301

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0001301

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

35084/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0001301

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

kel_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0001301

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0001301

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG7210

Flygut - An atlas of the Drosophila adult midgut

FBgn0001301

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0001301

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

35084

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG7210

MARRVEL_MODEL - MARRVEL (model organism gene)

35084

modMine - A data warehouse for the modENCODE project

FBgn0001301

Linkouts

BioGRID - A database of protein and genetic interactions.

61080

DroID - A comprehensive database of gene and protein interactions.

FBgn0001301

DRSC - Results frm RNAi screens

FBgn0001301

Developmental Studies Hybridoma Bank - Monoclonal antibodies for use in research

kel_1B

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0001301

FlyMine - An integrated database for Drosophila genomics

FBgn0001301

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

MIST (protein-protein) - An integrated Molecular Interaction Database

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

CG7210

(Meadows, 2017.1.11, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Ni et al., 2008, Rogers et al., 2003, Goldstein and Gunawardena, 2000)

Dm.Ring_canal/KELC_DROME

(Stogios and Prive, 2004)

Kel

(Morawe et al., 2011, Martin et al., 2007, Robins et al., 2005)

Kelch

(Bernard et al., 2018, Copf, 2015, Mai et al., 2004, Kelso et al., 2001)

kelch

(Schüpbach, 2019, Chen et al., 2011, Chintapalli et al., 2007)

Secondary FlyBase IDs

Datasets (8)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (8)

Result

Result Type

Title

scRNAseq_2020_Cattenoz_NI_seq_clustering

cell clustering analysis

Clustering analysis of hemocytes from non-infested third instar larvae

scRNAseq_2020_Cattenoz_WI_seq_clustering

cell clustering analysis

Clustering analysis of hemocytes from wasp-infested third instar larvae

scRNAseq_2020_Cho_NI72LG_seq_clustering

cell clustering analysis

Clustering analysis of lymph gland cells from non-infested larvae at 72 h after egg-laying

scRNAseq_2020_Cho_NI96LG_seq_clustering

cell clustering analysis

Clustering analysis of lymph gland cells from non-infested larvae at 96 h after egg-laying

scRNAseq_2020_Cho_NI120LG_seq_clustering

cell clustering analysis

Clustering analysis of lymph gland cells from non-infested larvae at 120 h after egg-laying

scRNAseq_2020_Cho_WI96LG_seq_clustering

cell clustering analysis

Clustering analysis of lymph gland cells from wasp-infested larvae at 96 h after egg-laying

scRNAseq_2020_Cho_NI96HL_seq_clustering

cell clustering analysis

Clustering analysis of circulating hemocytes from non-infested larvae at 96 h after egg-laying

scRNAseq_2020_Cho_NI120HL_seq_clustering

cell clustering analysis

Clustering analysis of circulating hemocytes from non-infested larvae at 120 h after egg-laying

References (149)

The following external sites may use different assemblies or annotations than FlyBase.

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