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Gene Dmel\kl-2

General Information
Symbol	Dmel\kl-2	Species	D. melanogaster
Name	male fertility factor kl2	Annotation symbol	CG17866
Feature type	protein_coding_gene	FlyBase ID	FBgn0001313
Gene Model Status	Incomplete	Stock availability	None publicly available
Genomic Location
Chromosome (arm)	YHet	Recombination map	Y-
Cytogenetic map	h10-h11	Sequence location	YHet:81,209..148,249 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene male fertility factor kl2 is referred to in FlyBase by the symbol Dmel\kl-2 (CG17866, FBgn0001313). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: ATPase activity, coupled; motor activity. An electronic pipeline based on InterPro domains suggests that it is involved in the biological process: microtubule-based movement. 17 alleles are reported. The phenotypes of these alleles are annotated with: Nebenkern; spermatid; spermatocyte. It has one annotated transcript and one annotated polypeptide. Protein features are: ATPase, AAA+ type, core; ATPase, dynein-related, AAA domain; Dynein heavy chain; Dynein heavy chain, P-loop containing D4 domain; Dynein heavy chain, coiled coil stalk; Dynein heavy chain, domain-1; Dynein heavy chain, domain-2. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very low expression to a trough of no expression detected. Peak expression observed during late larval stages, at stages throughout the pupal period, in adult male stages. Summary of FlyAtlas Anatomical Expression Data: No FlyAtlas data available because no Affy2 ProbeSet aligns to an exon of kl-2. Comments on Affy2 ProbeSet: No relevant information because no Affy2 ProbeSet aligns to an exon of kl-2. Gene sequence location is YHet:81209..148249.
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	kl-2 Males deficient or mutant for kl-2 are sterile; primary ultrastructural lesion not identified. Such sterile males lack a 300-325 kilodalton sperm polypeptide thought to be a structural component of the axoneme (Goldstein, Hardy, and Lindsley, 1982, Proc. Nat. Acad. Sci. USA 79: 7404-09; Hardy, Lindsley, Livak, Lewis, Sivertsen, Joslyn, Edwards, and Bonaccorsi, 1984, Genetics 107: 591-610).
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2011_10	Transgenic Constructs P{GD8807}
FB2012_01	Sequence features BKN40002 HFA21136
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location h10-h11   Left limit from complementation mapping against T(Y;2)R155 (FBrf0039037) Right limit from complementation mapping against In(YL)S16L (FBrf0039037)
Experimentally Determined Cytological Location
Cytogenetic map Notes References h10-h11     (Drosophila Heterochromatin Genome Project, 2004)
Experimentally Determined Recombination Data
Location	Y-
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\kl-2 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model is incomplete due to gap(s) in genomic sequence.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) kl-2-RB FBtr0300022  NM_001015505   12450   4146
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein kl-2-PB   FBpp0289299   481.3   4146   6.69     NP_001015505   EAL24531
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Dynein heavy chain (IPR004273) ATPase, dynein-related, AAA domain (IPR011704) Dynein heavy chain, domain-1 (IPR013594) Dynein heavy chain, domain-2 (IPR013602) ATPase, AAA+ type, core (IPR003593) Dynein heavy chain, P-loop containing D4 domain (IPR024317) Dynein heavy chain, coiled coil stalk (IPR024743)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AF313479   BG637093   CO288264   DQ239724 ABB76244   DQ239731 ABB76248   CM000461 EAL24531   EU685283   GH291272   GH291312
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q281W6 Q281X0 Q9GPN8 Q5LJP0
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0001313    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0001313 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very low expression to a trough of no expression detected. Peak expression observed during late larval stages, at stages throughout the pupal period, in adult male stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0001313 expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    7 larva L3 puffstage 7-9    13 white prepupae new    16 white prepupae 12hr    26 white prepupae 24hr    32 pupae 2d postWPP    50 pupae 3d postWPP    38 pupae 4d postWPP    11 adult male 01day    45 adult male 05day    39 adult male 30day    23 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  None   Extremely low   Very low  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    7 larva L3 puffstage 7-9    13 white prepupae new    16 white prepupae 12hr    26 white prepupae 24hr    32 pupae 2d postWPP    50 pupae 3d postWPP    38 pupae 4d postWPP    11 adult male 01day    45 adult male 05day    39 adult male 30day    23 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    7 larva L3 puffstage 7-9    13 white prepupae new    16 white prepupae 12hr    26 white prepupae 24hr    32 pupae 2d postWPP    50 pupae 3d postWPP    38 pupae 4d postWPP    11 adult male 01day    45 adult male 05day    39 adult male 30day    23 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    7 larva L3 puffstage 7-9    13 white prepupae new    16 white prepupae 12hr    26 white prepupae 24hr    32 pupae 2d postWPP    50 pupae 3d postWPP    38 pupae 4d postWPP    11 adult male 01day    45 adult male 05day    39 adult male 30day    23 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    7 larva L3 puffstage 7-9    13 white prepupae new    16 white prepupae 12hr    26 white prepupae 24hr    32 pupae 2d postWPP    50 pupae 3d postWPP    38 pupae 4d postWPP    11 adult male 01day    45 adult male 05day    39 adult male 30day    23 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0001313 expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    7 larva L3 puffstage 7-9    13 white prepupae new    16 white prepupae 12hr    26 white prepupae 24hr    32 pupae 2d postWPP    50 pupae 3d postWPP    38 pupae 4d postWPP    11 adult male 01day    45 adult male 05day    39 adult male 30day    23 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  None   Extremely low   Very low  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    7 larva L3 puffstage 7-9    13 white prepupae new    16 white prepupae 12hr    26 white prepupae 24hr    32 pupae 2d postWPP    50 pupae 3d postWPP    38 pupae 4d postWPP    11 adult male 01day    45 adult male 05day    39 adult male 30day    23 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    7 larva L3 puffstage 7-9    13 white prepupae new    16 white prepupae 12hr    26 white prepupae 24hr    32 pupae 2d postWPP    50 pupae 3d postWPP    38 pupae 4d postWPP    11 adult male 01day    45 adult male 05day    39 adult male 30day    23 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    7 larva L3 puffstage 7-9    13 white prepupae new    16 white prepupae 12hr    26 white prepupae 24hr    32 pupae 2d postWPP    50 pupae 3d postWPP    38 pupae 4d postWPP    11 adult male 01day    45 adult male 05day    39 adult male 30day    23 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    7 larva L3 puffstage 7-9    13 white prepupae new    16 white prepupae 12hr    26 white prepupae 24hr    32 pupae 2d postWPP    50 pupae 3d postWPP    38 pupae 4d postWPP    11 adult male 01day    45 adult male 05day    39 adult male 30day    23 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0001313 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG40099-RA;CG17866-RA;CG40099-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable, with Scer\GAL4pnr-MD237 kl-2GD8807 Sterility Allele male sterile kl-2A49 kl-2B37 kl-2B38 kl-2E5 kl-2E6 kl-2E7 kl-2E8 kl-2G7 kl-2S16L kl-2S18 Phenotype manifest in Allele Nebenkern kl-2E6 spermatid kl-2E6 spermatocyte kl-2E6
Classical Alleles ( 16 )
For All Classical Alleles Show
Allele of kl-2 Class Mutagen Stocks Known lesion kl-2A33 0 -- kl-2A49 0 -- kl-2B37 X ray irradiation 0 -- kl-2B38 X ray 0 -- kl-2E5 ethyl methanesulfonate 0 -- kl-2E6 ethyl methanesulfonate 0 -- kl-2E7 ethyl methanesulfonate 0 -- kl-2E8 ethyl methanesulfonate 0 -- kl-2G7 ethyl methanesulfonate gamma ray 0 -- kl-2G8 ethyl methanesulfonate 0 -- kl-2S16L gamma ray 0 -- kl-2S18 gamma ray 0 -- kl-2ts1 ethyl methanesulfonate 0 -- kl-2ts2 ethyl methanesulfonate 0 -- kl-2ts3 ethyl methanesulfonate 0 -- kl-2X1 X ray 0 --
Alleles Carried on Transgenic Constructs ( 1 )
For All Alleles Carried on Transgenic Constructs Show
Allele of kl-2 Class Mutagen Stocks Known lesion kl-2GD8807 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(YL)A10 (Hazelrigg et al., 1982) Df(YL)A17 (Hazelrigg et al., 1982) Df(YL)A49 (Hazelrigg et al., 1982) Df(YL)B14 (Hazelrigg et al., 1982) Df(YL)G19 (Hardy et al., 1984) Df(YL)G20 (Hardy et al., 1984) Df(YL)G21 (Hardy et al., 1984) Df(YL)G22 (Hardy et al., 1984) Df(YL)G23 (Hardy et al., 1984) Df(YL)G24 (Hardy et al., 1984) Df(YL)H121 (Bonaccorsi and Lohe, 1991) Df(YL)S9 (Gatti and Pimpinelli, 1983) Df(YL)S10 (Gatti and Pimpinelli, 1983) Df(YL)S11 (Gatti and Pimpinelli, 1983) Df(YL)S14 (Gatti and Pimpinelli, 1983) In(YL)S16L (Gatti and Pimpinelli, 1983) In(YLS)A33 (Hazelrigg et al., 1982) In(YLS)S18 (Gatti and Pimpinelli, 1983, Hardy et al., 1984) T(1;Y)R5   T(1;Y)R19   T(1;Y)V30   T(Y;2)R155 (Gatti and Pimpinelli, 1983, Hardy et al., 1984) Ts(1Lt;YSt)E15 (Zhang and Stankiewicz, 1998) Ts(1Lt;YSt)E15+Ts(YLt;3Lt)W27   Ts(1Lt;YSt)F12 (Zhang and Stankiewicz, 1998) Ts(1Rt;YLt)V24 (Bonaccorsi et al., 1990) Ts(1Rt;YSt)V8 (Bonaccorsi et al., 1990) Ts(1Rt;YSt)W19 (Bonaccorsi et al., 1990) Ts(YLt;3Lt)W27 (Bonaccorsi et al., 1990) Ts(YSt;4Rt)B15 (Hazelrigg et al., 1982)
Not disrupted in	Df(YL)A11 (Hazelrigg et al., 1982) Df(YL)A32 (Hazelrigg et al., 1982) Df(YL)A39 (Hazelrigg et al., 1982) Df(YL)B4 (Hazelrigg et al., 1982) Df(YL)B12 (Hazelrigg et al., 1982) Df(YL)S5 (Gatti and Pimpinelli, 1983) Df(YL)S6 (Gatti and Pimpinelli, 1983) Df(YL)S7 (Gatti and Pimpinelli, 1983) Df(YL)S8 (Gatti and Pimpinelli, 1983) Df(YL)S13 (Gatti and Pimpinelli, 1983) Df(YS)A6 (Hazelrigg et al., 1982) Df(YS)B13 (Hazelrigg et al., 1982) In(YL)B2 (Gatti and Pimpinelli, 1983) In(YL)B3 (Gatti and Pimpinelli, 1983) In(YLS)B1 (Gatti and Pimpinelli, 1983) T(1;Y)V24 (Gatti and Pimpinelli, 1983) T(Y;2)A169 (Gatti and Pimpinelli, 1983) T(Y;2)B3 (Hazelrigg et al., 1982) T(Y;2)B13 (Hazelrigg et al., 1982) T(Y;2)B92 (Gatti and Pimpinelli, 1983) T(Y;2)B242 (Gatti and Pimpinelli, 1983) T(Y;2)G74 (Gatti and Pimpinelli, 1983) T(Y;2)J146 (Gatti and Pimpinelli, 1983) T(Y;2)P57 (Gatti and Pimpinelli, 1983) T(Y;3)A23 (Gatti and Pimpinelli, 1983) T(Y;3)A34 (Hazelrigg et al., 1982) T(Y;3)A112 (Gatti and Pimpinelli, 1983) T(Y;3)B115 (Gatti and Pimpinelli, 1983) T(Y;3)B223 (Gatti and Pimpinelli, 1983) T(Y;3)B229 (Gatti and Pimpinelli, 1983) T(Y;3)D107 (Gatti and Pimpinelli, 1983) T(Y;3)S20 (Gatti and Pimpinelli, 1983) T(Y;3)S21 (Gatti and Pimpinelli, 1983) Ts(1Lt;YLt)V8 (Zhang and Stankiewicz, 1998) Ts(1Lt;YLt)W19 (Zhang and Stankiewicz, 1998) Ts(1Lt;YSt)V24 (Zhang and Stankiewicz, 1998) Ts(1Lt;YSt)W27 (Zhang and Stankiewicz, 1998) Ts(YLt;3Rt)A34 (Hazelrigg et al., 1982) Ts(YLt;4Lt)B15 (Hazelrigg et al., 1982)
Partially disrupted in	In(YL)S15 (Gatti and Pimpinelli, 1983)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data characterization construct P{GD8807} NA
Insertions
	Type of insertions Name Expression data
Gene Ontology: Function, Process & Cellular Component ( 8 unique terms )
Terms Based on Experimental Evidence ( 0 terms )
Terms Based on Predictions or Assertions ( 8 terms )
Molecular Function
CV term Evidence References
ATPase activity, coupled inferred from sequence or structural similarity with EMBL:AJ242523 AND traceable author statement (de Carvalho et al., 2000) ATP binding inferred from electronic annotation with InterPro:IPR011704 (FlyBase Curators et al., 2004-) microtubule motor activity inferred from electronic annotation with InterPro:IPR004273 (FlyBase Curators et al., 2004-) motor activity inferred from sequence or structural similarity with EMBL:AL034345 (FlyBase, 1992-)
Biological Process
CV term Evidence References
microtubule-based movement inferred from electronic annotation with InterPro:IPR004273 (FlyBase Curators et al., 2004-)
Cellular Component
CV term Evidence References
axonemal dynein complex inferred from sequence or structural similarity with EMBL:AL034345 (FlyBase, 1992-) dynein complex traceable author statement (de Carvalho et al., 2000) microtubule associated complex inferred from sequence or structural similarity with EMBL:AL034345 (FlyBase, 1992-)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions kl-2 allele Gene References
External Data
Linkouts	DroID - A comprehensive database of gene and protein interactions. • FBgn0001313
Orthologs
Genome-wide drosophilid orthologs
Curated drosophilid orthologs	Dbip\kl-2 (Kopp et al., 2005.10.14) Dmat\kl-2 (Kopp et al., 2005.10.14) Dmau\kl-2 (Kopp et al., 2005.10.14) Dnin\kl-2 (Kopp et al., 2005.10.14) Dpbp\kl-2 (Kopp et al., 2005.10.14) Dpse\kl-2 (Carvalho and Clark, 2005) Dpsp\Dkl-2 (Kopp et al., 2005.10.14) Dsec\kl-2 (Kopp et al., 2005.10.14) Dsim\kl-2 (Kopp et al., 2005.10.14)
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • XP_396228.3 Apis mellifera (honey bee) XP_001942638.1 Acyrthosiphon pisum (pea aphid) 91203 Batrachochytrium dendrobatidis (chytrid fungus) 124831 Branchiostoma floridae (lancelet) BGIBMGA010475-PA Bombyx mori (silk worm) ENSBTAP00000006277 Bos taurus (bovine) ENSCAFP00000024712 Canis familiaris (dog) ENSCINP00000017162 Ciona intestinalis (sea squirt) ENSCPOP00000003958 Cavia porcellus (guinea pig) 137221 Chlamidomonas reinhardtii (green alga) ENSCSAVP00000017295 Ciona savignyi (sedentary tunicate) 225405 Capitella spl (polychaete worm) ENSECAP00000007661 Equus caballus (horse) ENSGACP00000016392 Gymnopilus aculeatus (mushroom) GL50803_94440 Giardia lamblia (flagellated protozoan) ENSP00000340658 Homo sapiens (human) 184938 Lottia gigantea (owl limpet) LmjF23.1310 Leishmania major (virus) 20211 Monocosta brevicollis (choanoflagellate) ENSMODP00000009183 Monodelphis domestica (gray opossum) ENSMMUP00000010694 Macaca mulatta (rhesus monkey) ENSMUSP00000104299 Mus musculus (house mouse) 173194 Nematostella vectensis (starlet anemone) XP_001604907.1 Nasonia vitripennis (wasp) ENSORLP00000004700 Oryzias latipes (japanese killifish) 21159 Ostreococcus tauri (green alga) PHUM009272-PA Pediculus humanus (human body louse) 151766 Physcomitrella patens (moss) ENSPPYP00000008924 Pongo pygmaeus (orangutan) 46973 Phytophthora ramorum (sudden oak death pathogen) 133667 Phytophthora sojae (root rot pathogen) ENSPTRP00000014844 Pan troglodytes (chimpanzee) ENSRNOP00000047546 Rattus norvegicus (norway rat) Smp_176300 Schistosoma mansoni (trematode parasite) 23475 Trichoplax adhaerens (metazoa) XP_967358.2 Tribolium castaneum (red flour beetle) ENSTNIP00000022057 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000024656 Takifugu rubripes (pufferfish Takifugu) 162.m00065 Tetrahymena thermophila (ciliate protozoa) 95509.m00057 Trichomonas vaginalis (parasitic flagellated protozoan) ENSXETP00000018194 Xenopus tropicalis (frog) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV16466 LH18098 BGIBMGA010475 ACYPI002075 GB30008 GB15761 PB25327 PB25329 PHUM402310 GLEAN_12013 GLEAN_12470
Stocks & Reagents
Stocks Listed in FlyBase ( 0 )
Genomic Clones ( 0 )
cDNA Clones ( 3 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	SD14952
Other clones	DMG6-chrYHet.1.003.pred.a EK068112
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0001313 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 3355181
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: kl-2 CG17866 (FlyBase, 1996-)
Additional comments	Source for merge of kl-2 CG17866 was sequence comparison (date:001108). (FlyBase, 1996-) Williamson (1972) reported five EMS induced complementing alleles.
Other Comments
	Transiently named CG40438 in release 3 of the genome annotation. (FlyBase Genome Annotators, 2002-2003) Males deficient or mutant for kl-2 are sterile; primary ultrastructural lesion not identified. Such sterile males lack a 300-325 kilodalton sperm polypeptide thought to be a structural component of the axoneme (Goldstein, Hardy and Lindsley, 1982; Hardy, Lindsley, Livak, Lewis, Sivertsen, Joslyn, Edwards and Bonaccorsi, 1984).
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_001015505 RefSeq (Proteins) • NP_001015505 DDBJ / EMBL / GenBank • AAG29545 ABB76244 ABB76248 AF313479 DQ239724 DQ239731 EAL24531 EU685283 Entrez Gene - A searchable database of RefSeq genes. • 3355181 UniProtKB/TrEMBL • Q5LJP0 Q9GPN8 Q281W6 Q281X0
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Dynein heavy chain (IPR004273) ATPase, dynein-related, AAA domain (IPR011704) Dynein heavy chain, domain-1 (IPR013594) Dynein heavy chain, domain-2 (IPR013602) ATPase, AAA+ type, core (IPR003593) Dynein heavy chain, P-loop containing D4 domain (IPR024317) Dynein heavy chain, coiled coil stalk (IPR024743)
Linkouts
	DroID - A comprehensive database of gene and protein interactions. • FBgn0001313 DRSC - Results from RNAi screens. • FBgn0001313 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0001313 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG40099-RA;CG17866-RA;CG40099-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0001313 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 3355181 InParanoid A subset of ortholog calls from InParanoid. • XP_396228.3 Apis mellifera (honey bee) XP_001942638.1 Acyrthosiphon pisum (pea aphid) 91203 Batrachochytrium dendrobatidis (chytrid fungus) 124831 Branchiostoma floridae (lancelet) BGIBMGA010475-PA Bombyx mori (silk worm) ENSBTAP00000006277 Bos taurus (bovine) ENSCAFP00000024712 Canis familiaris (dog) ENSCINP00000017162 Ciona intestinalis (sea squirt) ENSCPOP00000003958 Cavia porcellus (guinea pig) 137221 Chlamidomonas reinhardtii (green alga) ENSCSAVP00000017295 Ciona savignyi (sedentary tunicate) 225405 Capitella spl (polychaete worm) ENSECAP00000007661 Equus caballus (horse) ENSGACP00000016392 Gymnopilus aculeatus (mushroom) GL50803_94440 Giardia lamblia (flagellated protozoan) ENSP00000340658 Homo sapiens (human) 184938 Lottia gigantea (owl limpet) LmjF23.1310 Leishmania major (virus) 20211 Monocosta brevicollis (choanoflagellate) ENSMODP00000009183 Monodelphis domestica (gray opossum) ENSMMUP00000010694 Macaca mulatta (rhesus monkey) ENSMUSP00000104299 Mus musculus (house mouse) 173194 Nematostella vectensis (starlet anemone) XP_001604907.1 Nasonia vitripennis (wasp) ENSORLP00000004700 Oryzias latipes (japanese killifish) 21159 Ostreococcus tauri (green alga) PHUM009272-PA Pediculus humanus (human body louse) 151766 Physcomitrella patens (moss) ENSPPYP00000008924 Pongo pygmaeus (orangutan) 46973 Phytophthora ramorum (sudden oak death pathogen) 133667 Phytophthora sojae (root rot pathogen) ENSPTRP00000014844 Pan troglodytes (chimpanzee) ENSRNOP00000047546 Rattus norvegicus (norway rat) Smp_176300 Schistosoma mansoni (trematode parasite) 23475 Trichoplax adhaerens (metazoa) XP_967358.2 Tribolium castaneum (red flour beetle) ENSTNIP00000022057 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000024656 Takifugu rubripes (pufferfish Takifugu) 162.m00065 Tetrahymena thermophila (ciliate protozoa) 95509.m00057 Trichomonas vaginalis (parasitic flagellated protozoan) ENSXETP00000018194 Xenopus tropicalis (frog) modMine - Data generated by the modENCODE project. • FBgn0001313 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV16466 LH18098 BGIBMGA010475 ACYPI002075 GB30008 GB15761 PB25327 PB25329 PHUM402310 GLEAN_12013 GLEAN_12470
Synonyms & Secondary IDs ( 6 )
Reported As
Symbol Synonym	CG17866 (Drosophila Heterochromatin Genome Project, 2007) CG40438   kl-2 (Vibranovski et al., 2008, Koerich et al., 2007, Carvalho and Clark, 2005, Larracuente and Clark, 2008, Diaz-Castillo and Golic, 2007, Bachtrog et al., 2006, FlyBase, 1996-, Koerich et al., 2008, Ding et al., 2010) Lms2 (Hazelrigg et al., 1982, )
Name Synonym	1β-dynein heavy chain (Gvozdev et al., 2005) male fertility factor kl2
Secondary FlyBase IDs
	FBan0017866 FBgn0039968 FBgn0058438
References ( 60 )
Generate a list of	All references relating to this gene ( 60 )
List References by type	Research paper  ( 27 ) Supplementary material  ( 1 ) Review  ( 8 ) Personal communication to FlyBase  ( 5 ) Abstract  ( 3 ) FlyBase analysis  ( 3 ) DNA/RNA sequence record  ( 9 ) Curated genome annotation  ( 1 ) Automatic genome annotation  ( 1 ) Book  ( 2 )
Recent research papers ( 1 )
	Ding et al., 2010, PLoS Genet. 6(12): e1001255 A young Drosophila duplicate gene plays essential roles in spermatogenesis by regulating several y-linked male fertility genes. [FBrf0212639] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...