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Gene Dmel\kl-3

General Information
Symbol	Dmel\kl-3	Species	D. melanogaster
Name	male fertility factor kl3	Annotation symbol	CG17629
Feature type	protein_coding_gene	FlyBase ID	FBgn0001314
Gene Model Status	Incomplete	Stock availability	1 publicly available
Also Known As	kl3
Genomic Location
Chromosome (arm)	YHet	Recombination map	Y-
Cytogenetic map	h8-h8	Sequence location	YHet:34,290..65,839 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene male fertility factor kl3 is referred to in FlyBase by the symbol Dmel\kl-3 (CG17629, FBgn0001314). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: ATPase activity, coupled; motor activity. Based on sequence similarity, it is predicted to be involved in the biological process: microtubule-based movement. 24 alleles are reported. The phenotypes of these alleles are annotated with: spermatid; testis; spermatid axoneme; spermatozoon. It has one annotated transcript and one annotated polypeptide. Protein features are: ATPase, AAA+ type, core; ATPase, dynein-related, AAA domain; Dynein heavy chain; Dynein heavy chain, P-loop containing D4 domain; Dynein heavy chain, coiled coil stalk; Dynein heavy chain, domain-1; Dynein heavy chain, domain-2. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very low expression to a trough of no expression detected. Peak expression observed at stages throughout the pupal period, in adult male stages. Summary of FlyAtlas Anatomical Expression Data: No FlyAtlas data available because no Affy2 ProbeSet aligns to an exon of kl-3. Comments on Affy2 ProbeSet: No relevant information because no Affy2 ProbeSet aligns to an exon of kl-3. Gene sequence location is YHet:34290..65839.
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	kl-3 Males mutant for kl-3 fail to assemble the outer dynein arms associated with the peripheral nine microtubule doublets of the sperm-tail axoneme [Hardy, Tokuyasu, and Lindsley, 1981, Chromosoma 83: 593-617 (fig.)]. Such males also fail to produce a kl-3-specific 300-325 kilodalton sperm polypeptide presumed to be a component of the outer dynein arms (Goldstein, Hardy, and Lindsley, 1982, Proc. Nat. Acad. Sci. USA 79: 7404-09). Such mutants display an exceedingly low level of fertility at 25 but not at 18 immediately upon eclosion, but not thereafter (Kennison, 1983, Genetics 103: 219-34). Deficiencies for kl-3 are completely sterile, and in addition to the above mutant phenotype, they fail to elaborate the loops (ribbon-like structure) observed in the primary spermatocyte nuclei of normal males by light microscopy and the reticular material ordinarily observed by electron microscopy in spermatocyte nuclei (Hardy et al., 1981). The kl-3 loops are visible in living spermatocytes and in fixed cells stained with the protein-specific dye CBB; these loops are also demonstrated by the polyclonal antibody Sph-155 but do not react with monoclonal antibody S5 (Bonaccorsi et al., 1988).
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2011_10	Controlled Vocabulary Terms day 6 of adulthood day 5 of adulthood male day 7 of adulthood spermatozoon Transgenic Constructs P{GD9429}
FB2012_01	Sequence features BKN40003 HFA20730 HFA20891 HFA20934
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location h8-h8   Left limit from complementation mapping against T(Y;3)S20 (FBrf0039037) Right limit from complementation mapping against T(Y;3)D107 (FBrf0039037)
Experimentally Determined Cytological Location
Cytogenetic map Notes References h7-h9     (Drosophila Heterochromatin Genome Project, 2004) h7-h9   (determined by in situ hybridisation)   (Zhang and Stankiewicz, 1998) h7-h9     (Zhang et al., 1996)   Maps to the region of kl-3.   (Zhang, 1996.4.28)
Experimentally Determined Recombination Data
Location	Y- (Hackstein and Hochstenbach, 1995)
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\kl-3 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Multiple partial annotations correspond to portions of a single gene, kl-3; these are (5' to 3'): CG40992, [gap], CG40936, [gap], CG17629. Gene model is incomplete due to gap(s) in genomic sequence and fragmented genome assembly.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) kl-3-RA FBtr0113756  NM_001111012   8278   2691
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein kl-3-PA   FBpp0112479   308.9   2691   5.37     NP_001104482   EDP28010
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Dynein heavy chain (IPR004273) ATPase, dynein-related, AAA domain (IPR011704) Dynein heavy chain, domain-2 (IPR013602) ATPase, AAA+ type, core (IPR003593) Dynein heavy chain, domain-1 (IPR013594) Dynein heavy chain, P-loop containing D4 domain (IPR024317) Dynein heavy chain, coiled coil stalk (IPR024743)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AF313480   BI593087   CM000461 EDP28010   GH291244   GO271070   GO271145
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q9GPN7 A8Y5B7
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
mass spectroscopy Stage Tissue/Position (including subcellular localization) Reference day 5 of adulthood & male -- day 7 of adulthood & male   spermatozoon   (Wasbrough et al., 2010)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0001314    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0001314 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very low expression to a trough of no expression detected. Peak expression observed at stages throughout the pupal period, in adult male stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0001314 expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    5 larva L3 puffstage 7-9    7 white prepupae new    7 white prepupae 12hr    21 white prepupae 24hr    29 pupae 2d postWPP    54 pupae 3d postWPP    53 pupae 4d postWPP    15 adult male 01day    61 adult male 05day    45 adult male 30day    32 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  None   Extremely low   Very low  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    5 larva L3 puffstage 7-9    7 white prepupae new    7 white prepupae 12hr    21 white prepupae 24hr    29 pupae 2d postWPP    54 pupae 3d postWPP    53 pupae 4d postWPP    15 adult male 01day    61 adult male 05day    45 adult male 30day    32 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    5 larva L3 puffstage 7-9    7 white prepupae new    7 white prepupae 12hr    21 white prepupae 24hr    29 pupae 2d postWPP    54 pupae 3d postWPP    53 pupae 4d postWPP    15 adult male 01day    61 adult male 05day    45 adult male 30day    32 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    5 larva L3 puffstage 7-9    7 white prepupae new    7 white prepupae 12hr    21 white prepupae 24hr    29 pupae 2d postWPP    54 pupae 3d postWPP    53 pupae 4d postWPP    15 adult male 01day    61 adult male 05day    45 adult male 30day    32 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    5 larva L3 puffstage 7-9    7 white prepupae new    7 white prepupae 12hr    21 white prepupae 24hr    29 pupae 2d postWPP    54 pupae 3d postWPP    53 pupae 4d postWPP    15 adult male 01day    61 adult male 05day    45 adult male 30day    32 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0001314 expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    5 larva L3 puffstage 7-9    7 white prepupae new    7 white prepupae 12hr    21 white prepupae 24hr    29 pupae 2d postWPP    54 pupae 3d postWPP    53 pupae 4d postWPP    15 adult male 01day    61 adult male 05day    45 adult male 30day    32 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  None   Extremely low   Very low  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    5 larva L3 puffstage 7-9    7 white prepupae new    7 white prepupae 12hr    21 white prepupae 24hr    29 pupae 2d postWPP    54 pupae 3d postWPP    53 pupae 4d postWPP    15 adult male 01day    61 adult male 05day    45 adult male 30day    32 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    5 larva L3 puffstage 7-9    7 white prepupae new    7 white prepupae 12hr    21 white prepupae 24hr    29 pupae 2d postWPP    54 pupae 3d postWPP    53 pupae 4d postWPP    15 adult male 01day    61 adult male 05day    45 adult male 30day    32 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    5 larva L3 puffstage 7-9    7 white prepupae new    7 white prepupae 12hr    21 white prepupae 24hr    29 pupae 2d postWPP    54 pupae 3d postWPP    53 pupae 4d postWPP    15 adult male 01day    61 adult male 05day    45 adult male 30day    32 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    5 larva L3 puffstage 7-9    7 white prepupae new    7 white prepupae 12hr    21 white prepupae 24hr    29 pupae 2d postWPP    54 pupae 3d postWPP    53 pupae 4d postWPP    15 adult male 01day    61 adult male 05day    45 adult male 30day    32 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0001314 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG17629-RD
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable, with Scer\GAL4pnr-MD237 kl-3GD9429 Sterility Allele male sterile kl-328 kl-361 kl-3104b kl-3B11 kl-3P57 kl-3S16L kl-3S20 male sterile (with Ab(Y)Y2) kl-328 kl-361 kl-3104b male sterile (with Ab(Y)Y146) kl-328 kl-361 kl-3104b Phenotype manifest in Allele spermatid kl-3104b kl-328 kl-361 spermatid axoneme kl-3104b kl-328 kl-361 spermatozoon kl-3104b testis kl-3104b kl-328 kl-361
Classical Alleles ( 23 )
For All Classical Alleles Show
Allele of kl-3 Class Mutagen Stocks Known lesion kl-3P57 1 -- kl-3104b P-element activity 0 -- kl-328 Delta2-3 0 -- kl-361 Delta2-3 0 -- kl-3A29 X ray 0 -- kl-3A5 X ray 0 -- kl-3B11 X ray 0 -- kl-3B20 X ray 0 -- kl-3B27 X ray 0 -- kl-3B41 X ray 0 -- kl-3E10 ethyl methanesulfonate 0 -- kl-3E11 ethyl methanesulfonate 0 -- kl-3E9 ethyl methanesulfonate 0 -- kl-3G10 gamma ray 0 -- kl-3G11 gamma ray 0 -- kl-3G17 gamma ray 0 -- kl-3G9 gamma ray 0 -- kl-3S16L 0 -- kl-3S20 gamma ray 0 -- kl-3X1 X ray 0 -- kl-3X2 X ray 0 -- kl-3X3 X ray 0 -- kl-3X4 X ray 0 --
Alleles Carried on Transgenic Constructs ( 1 )
For All Alleles Carried on Transgenic Constructs Show
Allele of kl-3 Class Mutagen Stocks Known lesion kl-3GD9429 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(YL)A10 (Hazelrigg et al., 1982) Df(YL)A11 (Hazelrigg et al., 1982) Df(YL)A17 (Hazelrigg et al., 1982) Df(YL)A32 (Hazelrigg et al., 1982) Df(YL)A39 (Hazelrigg et al., 1982) Df(YL)A49 (Hazelrigg et al., 1982) Df(YL)B4 (Hazelrigg et al., 1982) Df(YL)B12 (Hazelrigg et al., 1982) Df(YL)B14 (Hazelrigg et al., 1982) Df(YL)G19 (Hardy et al., 1984) Df(YL)G20 (Hardy et al., 1984) Df(YL)G21 (Hardy et al., 1984) Df(YL)G22 (Hardy et al., 1984) Df(YL)G23 (Hardy et al., 1984) Df(YL)G24 (Hardy et al., 1984) Df(YL)H121 (Bonaccorsi and Lohe, 1991) Df(YL)S7 (Gatti and Pimpinelli, 1983) Df(YL)S8 (Gatti and Pimpinelli, 1983) Df(YL)S9 (Gatti and Pimpinelli, 1983) Df(YL)S10 (Gatti and Pimpinelli, 1983) Df(YL)S11 (Gatti and Pimpinelli, 1983) Df(YL)S13 (Gatti and Pimpinelli, 1983) Df(YL)S14 (Gatti and Pimpinelli, 1983) In(YL)B2 (Gatti and Pimpinelli, 1983) In(YL)S15 (Gatti and Pimpinelli, 1983) In(YL)S16L (Gatti and Pimpinelli, 1983) T(1;Y;3)W27 (Piergentili et al., 2004) T(1;Y)R39   T(1;Y)S1   T(1;Y)S2   T(1;Y)S24   T(1;Y)T13 (Bonaccorsi and Lohe, 1991) T(1;Y)V24 (Piergentili et al., 2004) T(1;Y)V57   T(Y;2)G74 (Gatti and Pimpinelli, 1983, Bonaccorsi and Lohe, 1991) T(Y;2)P57 (Gatti and Pimpinelli, 1983, Bonaccorsi and Lohe, 1991) T(Y;3)D107 (Gatti and Pimpinelli, 1983) T(Y;3)S20 (Gatti and Pimpinelli, 1983) Ts(1Lt;YSt)E15 (Zhang and Stankiewicz, 1998) Ts(1Lt;YSt)F12 (Zhang and Stankiewicz, 1998) Ts(1Lt;YSt)T13 (Bonaccorsi et al., 1990) Ts(1Lt;YSt)W27 (Zhang and Stankiewicz, 1998) Ts(1Rt;YLt)V24 (Bonaccorsi et al., 1990) Ts(1Rt;YSt)V8 (Bonaccorsi et al., 1990) Ts(1Rt;YSt)W19 (Bonaccorsi et al., 1990) Ts(YSt;4Rt)B15 (Hazelrigg et al., 1982)
Not disrupted in	Df(YL)S5 (Gatti and Pimpinelli, 1983) Df(YL)S6 (Gatti and Pimpinelli, 1983) Df(YS)A6 (Hazelrigg et al., 1982) Df(YS)B13 (Hazelrigg et al., 1982) In(YL)B3 (Gatti and Pimpinelli, 1983) In(YLS)A33 (Hazelrigg et al., 1982) In(YLS)B1 (Gatti and Pimpinelli, 1983) In(YLS)S18 (Gatti and Pimpinelli, 1983) T(1;Y)V24 (Gatti and Pimpinelli, 1983) T(Y;2)A169 (Gatti and Pimpinelli, 1983) T(Y;2)B3 (Hazelrigg et al., 1982) T(Y;2)B13 (Hazelrigg et al., 1982) T(Y;2)B92 (Gatti and Pimpinelli, 1983) T(Y;2)B242 (Gatti and Pimpinelli, 1983) T(Y;2)J146 (Gatti and Pimpinelli, 1983) T(Y;2)R155 (Gatti and Pimpinelli, 1983) T(Y;3)A23 (Gatti and Pimpinelli, 1983) T(Y;3)A34 (Hazelrigg et al., 1982) T(Y;3)A112 (Gatti and Pimpinelli, 1983) T(Y;3)B115 (Gatti and Pimpinelli, 1983) T(Y;3)B223 (Gatti and Pimpinelli, 1983) T(Y;3)B229 (Gatti and Pimpinelli, 1983) T(Y;3)S21 (Gatti and Pimpinelli, 1983) Ts(1Lt;YLt)V8 (Zhang and Stankiewicz, 1998) Ts(1Lt;YLt)W19 (Zhang and Stankiewicz, 1998) Ts(1Lt;YSt)V24 (Zhang and Stankiewicz, 1998) Ts(YLt;3Lt)W27 (Bonaccorsi et al., 1990) Ts(YLt;3Rt)A34 (Hazelrigg et al., 1982) Ts(YLt;4Lt)B15 (Hazelrigg et al., 1982)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data characterization construct P{GD9429} NA
Insertions
	Type of insertions Name Expression data insertion of enhancer trap P{PZ}kl-328 No P{PZ}kl-361 No P{PZ}kl-3104b No
Gene Ontology: Function, Process & Cellular Component ( 8 unique terms )
Terms Based on Experimental Evidence ( 0 terms )
Terms Based on Predictions or Assertions ( 8 terms )
Molecular Function
CV term Evidence References
ATPase activity, coupled inferred from sequence or structural similarity (Goldstein and Gunawardena, 2000) inferred from sequence or structural similarity with CG9492 AND traceable author statement (de Carvalho et al., 2000) ATP binding inferred from electronic annotation with InterPro:IPR011704 (FlyBase Curators et al., 2004-) microtubule binding non-traceable author statement (de Carvalho, 2000.10.15) microtubule motor activity inferred from electronic annotation with InterPro:IPR004273 (FlyBase Curators et al., 2004-) motor activity inferred from sequence or structural similarity with EMBL:AL034345 (FlyBase, 1992-)
Biological Process
CV term Evidence References
microtubule-based movement inferred from electronic annotation with InterPro:IPR004273 (FlyBase Curators et al., 2004-) inferred from sequence or structural similarity (Goldstein and Gunawardena, 2000)
Cellular Component
CV term Evidence References
axonemal dynein complex inferred from sequence or structural similarity (Goldstein and Gunawardena, 2000) inferred from sequence or structural similarity with EMBL:AL034345 (FlyBase, 1992-) non-traceable author statement (de Carvalho, 2000.10.15) microtubule associated complex inferred from sequence or structural similarity with EMBL:AL034345 (FlyBase, 1992-)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions kl-3 allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 625284 DroID - A comprehensive database of gene and protein interactions. • FBgn0001314 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 5740653
Orthologs
Genome-wide drosophilid orthologs
Curated drosophilid orthologs	Dafs\kl-3 (Carvalho and Clark, 2005) Dazt\kl-3 (Carvalho and Clark, 2005) Dbif\kl-3 (Carvalho and Clark, 2005) Dbii\kl-3   Dele\kl-3   Deug\kl-3   Dfuy\kl-3   Dgua\kl-3 (Carvalho and Clark, 2005) Dluc\kl-3   Dmim\kl-3   Dpse\kl-3 (Carvalho and Clark, 2005) Dsan\CG17629 (Llopart et al., 2005) Dsuz\kl-3   Dyak\CG17629 (Llopart et al., 2005)
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • CN22680 Caenorhabditis brenneri (small nematode) PP25337 Pristionchus pacificus (nematode)
Stocks & Reagents
Stocks Listed in FlyBase ( 1 )
Bloomington	2756 T(Y;2)P57, BS, y+ kl-3P57/SM1; C(1)RM, y1/C(1;Y)1, y1
Genomic Clones ( 0 )
cDNA Clones ( 3 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	RH10985
Other clones	DMG5-chrYHet.1.001.pred.a FB59-CG17629-RA
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0001314 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 3355177
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: kl-3 CG17629 (FlyBase, 1996-)
Additional comments	Source for merge of kl-3 CG17629 was sequence comparison (date:001108). (FlyBase, 1996-) Williamson (1972) reported 23 non-complementing and nine complementing alleles induced by EMS.
Other Comments
	Transiently named CG40443 in release 3 of the genome annotation. (FlyBase Genome Annotators, 2002-2003) It is not yet known if "ms(Y)104b" is the same as kl-3. (Zhang, 1996.4.28) Primary spermatocyte nuclei exhibit three giant lampbrush-like loops formed by the kl-5, kl-3 and ks-1 Y chromosome fertility factors. (Pisano et al., 1993) Males mutant for kl-3 fail to assemble the outer dynein arms associated with the peripheral nine microtubule doublets of the sperm-tail axoneme (Hardy, Tokuyasu, and Lindsley, 1981). Such males also fail to produce a kl-3-specific 300-325 kilodalton sperm polypeptide presumed to be a component of the outer dynein arms (Goldstein, Hardy and Lindsley, 198). Such mutants display an exceedingly low level of fertility at 25oC but not at 18oC immediately upon eclosion, but not thereafter (Kennison, 1983). Deficiencies for kl-3 are completely sterile, and in addition to the above mutant phenotype, they fail to elaborate the loops (ribbon-like structure) observed in the primary spermatocyte nuclei of normal males by light microscopy and the reticular material ordinarily observed by electron microscopy in spermatocyte nuclei (Hardy et al., 1981). The kl-3 loops are visible in living spermatocytes and in fixed cells stained with the protein-specific dye CBB; these loops are also demonstrated by the polyclonal antibody Sph-155 but do not react with monoclonal antibody S5 (Bonaccorsi et al., 1988).
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_001111012 RefSeq (Proteins) • NP_001104482 DDBJ / EMBL / GenBank • AAG29546 AF313480 EDP28010 Entrez Gene - A searchable database of RefSeq genes. • 5740653 UniProtKB/TrEMBL • A8Y5B7 Q9GPN7
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Dynein heavy chain (IPR004273) ATPase, dynein-related, AAA domain (IPR011704) Dynein heavy chain, domain-2 (IPR013602) ATPase, AAA+ type, core (IPR003593) Dynein heavy chain, domain-1 (IPR013594) Dynein heavy chain, P-loop containing D4 domain (IPR024317) Dynein heavy chain, coiled coil stalk (IPR024743)
Linkouts
	BioGRID - A database of protein and genetic interactions • 625284 DroID - A comprehensive database of gene and protein interactions. • FBgn0001314 DRSC - Results from RNAi screens. • FBgn0001314 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0001314 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG17629-RD FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0001314 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 3355177 InParanoid A subset of ortholog calls from InParanoid. • CN22680 Caenorhabditis brenneri (small nematode) PP25337 Pristionchus pacificus (nematode) InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 5740653 modMine - Data generated by the modENCODE project. • FBgn0001314
Synonyms & Secondary IDs ( 10 )
Reported As
Symbol Synonym	CG17629 (Goldstein and Gunawardena, 2000, Drosophila Heterochromatin Genome Project, 2007) CG40443 (Giot et al., 2003, ) kl3 (Zhang et al., 1998, Zhang et al., 1996) kl-3 (Koerich et al., 2007, Carvalho and Clark, 2005, Larracuente and Clark, 2008, Diaz-Castillo and Golic, 2007, Bachtrog et al., 2006, Koerich et al., 2008, Piergentili, 2006, Ding et al., 2010, Wasbrough et al., 2010, Wasbrough et al., 2010) kl-4 (Hackstein et al., 1996) Lms3 (Hazelrigg et al., 1982, ) ms(Y)104b   Y104b (Zhang et al., 1996)
Name Synonym	male fertility factor kl3 (Wasbrough et al., 2010) γ-dynein heavy chain (Gvozdev et al., 2005)
Secondary FlyBase IDs
	FBan0017629 FBgn0016045 FBgn0040039 FBgn0058443
References ( 65 )
Generate a list of	All references relating to this gene ( 65 )
List References by type	Research paper  ( 32 ) Supplementary material  ( 4 ) Review  ( 8 ) Personal communication to FlyBase  ( 5 ) Abstract  ( 8 ) FlyBase analysis  ( 3 ) DNA/RNA sequence record  ( 1 ) Automatic genome annotation  ( 1 ) Curated genome annotation  ( 1 ) Book  ( 2 )
Recent research papers ( 2 )
	Ding et al., 2010, PLoS Genet. 6(12): e1001255 A young Drosophila duplicate gene plays essential roles in spermatogenesis by regulating several y-linked male fertility genes. [FBrf0212639] Wasbrough et al., 2010, J. Proteomics 73(11): 2171--2185 The Drosophila melanogaster sperm proteome-II (DmSP-II). [FBrf0211978] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...