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Gene Dmel\kl-5

General Information
Symbol	Dmel\kl-5	Species	D. melanogaster
Name	male fertility factor kl5	Annotation symbol	CG40444
Feature type	protein_coding_gene	FlyBase ID	FBgn0001315
Gene Model Status	Incomplete	Stock availability	1 publicly available
Also Known As	Dhc-Yh3, CG17616, DhcYh3
Genomic Location
Chromosome (arm)	YHet	Recombination map	Y-
Cytogenetic map	h2-h3	Sequence location	YHet:311..33,413 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene male fertility factor kl5 is referred to in FlyBase by the symbol Dmel\kl-5 (CG40444, FBgn0001315). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: ATPase activity, coupled; motor activity; microtubule binding. Based on sequence similarity, it is predicted to be involved in the biological process: microtubule-based movement. 25 alleles are reported. The phenotypes of these alleles are annotated with: spermatid; testis; axoneme. It has one annotated transcript and one annotated polypeptide. Protein features are: ATPase, AAA+ type, core; ATPase, dynein-related, AAA domain; Dynein heavy chain; Dynein heavy chain, P-loop containing D4 domain; Dynein heavy chain, coiled coil stalk; Dynein heavy chain, domain-1; Dynein heavy chain, domain-2. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very low expression to a trough of no expression detected. Peak expression observed during late larval stages, at stages throughout the pupal period, in adult male stages. Summary of FlyAtlas Anatomical Expression Data: No FlyAtlas data available because no Affy2 ProbeSet aligns to an exon of kl-5. Comments on Affy2 ProbeSet: No relevant information because no Affy2 ProbeSet aligns to an exon of kl-5. Gene sequence location is YHet:311..33413.
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	kl-5 Males mutant for kl-5 fail to assemble the outer dynein arms associated with the A tubules of the peripheral doublets of the sperm tail axoneme [Hardy, Tokuyasu, and Lindsley, 1981, Chromosoma 83: 593-617 (fig.)]. Such males also fail to produce kl-5-specific 300-325 kilodalton sperm polypeptide considered to be a component of the outer arms (Goldstein, Hardy, and Lindsley, 1982, Proc. Nat. Acad. Sci. USA 79: 7404-09). kl-5 mutants exhibit very low level of fertility at 25 but not at 18 and in young but not older males (Kennison, 1983, Genetics 103: 219-34). Deficiencies for kl-5 completely sterile, and in addition to the mutant phenotype they fail to produce aggregates of tubuli normally seen in thin sections of primary spermatocyte nuclei (Hardy et al.). Within the kl-5 fertility region of the spermatocyte nucleus, a loop-forming site has been demonstrated by its reaction with the monoclonal antibody S5 (Bonaccorsi et al., 1988). These loops are stained with the protein-specific dye CBB, but are not visible in living preparations.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2011_10	Transgenic Constructs P{GD9426}
FB2012_01	Sequence features HFA20736 HFA20773 BKN40363 HFA21161 HFA20707
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location h2-h3   Left limit from complementation mapping against T(Y;3)G42 (FBrf0039037) Right limit from complementation mapping against T(Y;3)J94 (FBrf0039037)
Experimentally Determined Cytological Location
Cytogenetic map Notes References h2-h3     (Drosophila Heterochromatin Genome Project, 2004) h3-h3     (Gepner, 1993) h3-h3   (determined by in situ hybridisation)   (Rasmusson et al., 1994)
Experimentally Determined Recombination Data
Location	Y- (Hackstein and Hochstenbach, 1995, )
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\kl-5 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model is incomplete due to gap(s) in genomic sequence and fragmented genome assembly.. Multiple partial annotations correspond to a single gene, kl-5: CG40444 encodes most of gene (significant gaps); penultimate 3' exon represented by CG41497.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) kl-5-RA FBtr0113891  NM_001015499   7122   2373
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein kl-5-PA   FBpp0112614   274.6   2373   5.55     NP_001015499   EAL24525
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Dynein heavy chain (IPR004273) ATPase, dynein-related, AAA domain (IPR011704) Dynein heavy chain, domain-1 (IPR013594) Dynein heavy chain, domain-2 (IPR013602) ATPase, AAA+ type, core (IPR003593) Dynein heavy chain, P-loop containing D4 domain (IPR024317) Dynein heavy chain, coiled coil stalk (IPR024743)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AF136243   AF136244   AF136245   AF136246   AF136247   AF136248   AF136249   AF136250   AF136251   AF136252   AF136253   AF210453   CM000461 EAL24525   GH291374   L23199 AAA52099
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q9TW74 Q9U3Y5 Q5LJN5
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference adult stage | male   organism   (Rasmusson et al., 1994) Comment:kl-5[+] transcripts are detected only in adult males northern blot Stage Tissue/Position (including subcellular localization) Reference adult stage   testis   (Rasmusson et al., 1994)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
mass spectroscopy Stage Tissue/Position (including subcellular localization) Reference day 5 of adulthood & male -- day 7 of adulthood & male   spermatozoon   (Wasbrough et al., 2010)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0001315    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0001315 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very low expression to a trough of no expression detected. Peak expression observed during late larval stages, at stages throughout the pupal period, in adult male stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0001315 expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    7 larva L3 puffstage 7-9    12 white prepupae new    13 white prepupae 12hr    35 white prepupae 24hr    37 pupae 2d postWPP    55 pupae 3d postWPP    49 pupae 4d postWPP    21 adult male 01day    65 adult male 05day    58 adult male 30day    36 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  None   Extremely low   Very low  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    7 larva L3 puffstage 7-9    12 white prepupae new    13 white prepupae 12hr    35 white prepupae 24hr    37 pupae 2d postWPP    55 pupae 3d postWPP    49 pupae 4d postWPP    21 adult male 01day    65 adult male 05day    58 adult male 30day    36 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    7 larva L3 puffstage 7-9    12 white prepupae new    13 white prepupae 12hr    35 white prepupae 24hr    37 pupae 2d postWPP    55 pupae 3d postWPP    49 pupae 4d postWPP    21 adult male 01day    65 adult male 05day    58 adult male 30day    36 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    7 larva L3 puffstage 7-9    12 white prepupae new    13 white prepupae 12hr    35 white prepupae 24hr    37 pupae 2d postWPP    55 pupae 3d postWPP    49 pupae 4d postWPP    21 adult male 01day    65 adult male 05day    58 adult male 30day    36 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    7 larva L3 puffstage 7-9    12 white prepupae new    13 white prepupae 12hr    35 white prepupae 24hr    37 pupae 2d postWPP    55 pupae 3d postWPP    49 pupae 4d postWPP    21 adult male 01day    65 adult male 05day    58 adult male 30day    36 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0001315 expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    7 larva L3 puffstage 7-9    12 white prepupae new    13 white prepupae 12hr    35 white prepupae 24hr    37 pupae 2d postWPP    55 pupae 3d postWPP    49 pupae 4d postWPP    21 adult male 01day    65 adult male 05day    58 adult male 30day    36 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  None   Extremely low   Very low  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    7 larva L3 puffstage 7-9    12 white prepupae new    13 white prepupae 12hr    35 white prepupae 24hr    37 pupae 2d postWPP    55 pupae 3d postWPP    49 pupae 4d postWPP    21 adult male 01day    65 adult male 05day    58 adult male 30day    36 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    7 larva L3 puffstage 7-9    12 white prepupae new    13 white prepupae 12hr    35 white prepupae 24hr    37 pupae 2d postWPP    55 pupae 3d postWPP    49 pupae 4d postWPP    21 adult male 01day    65 adult male 05day    58 adult male 30day    36 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    7 larva L3 puffstage 7-9    12 white prepupae new    13 white prepupae 12hr    35 white prepupae 24hr    37 pupae 2d postWPP    55 pupae 3d postWPP    49 pupae 4d postWPP    21 adult male 01day    65 adult male 05day    58 adult male 30day    36 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    7 larva L3 puffstage 7-9    12 white prepupae new    13 white prepupae 12hr    35 white prepupae 24hr    37 pupae 2d postWPP    55 pupae 3d postWPP    49 pupae 4d postWPP    21 adult male 01day    65 adult male 05day    58 adult male 30day    36 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0001315 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG40444-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable, with Scer\GAL4pnr-MD237 kl-5GD9426 Sterility Allele male fertile | reduced | heat sensitive kl-5ts2 male sterile kl-516 kl-5B3 kl-5E17 kl-5G53 kl-5R50 kl-5S21 male sterile (with Ab(Y)Y2) kl-516 male sterile (with Ab(Y)Y146) kl-516 Phenotype manifest in Allele axoneme kl-5ts2 spermatid kl-516 testis kl-516
Classical Alleles ( 24 )
For All Classical Alleles Show
Allele of kl-5 Class Mutagen Stocks Known lesion kl-5R50 X ray 1 -- kl-516 Delta2-3 0 -- kl-5B28 X ray 0 -- kl-5B34 X ray 0 -- kl-5B3 X ray 0 -- kl-5E12 ethyl methanesulfonate 0 -- kl-5E13 ethyl methanesulfonate 0 -- kl-5E14 ethyl methanesulfonate 0 -- kl-5E15 ethyl methanesulfonate 0 -- kl-5E16 ethyl methanesulfonate 0 -- kl-5E17 ethyl methanesulfonate 0 -- kl-5G12 gamma ray 0 -- kl-5G13 gamma ray 0 -- kl-5G14 gamma ray 0 -- kl-5G15 gamma ray 0 -- kl-5G16 gamma ray 0 -- kl-5G53 X ray 0 -- kl-5S21 gamma ray 0 -- kl-5ts1 ethyl methanesulfonate 0 -- kl-5ts2 ethyl methanesulfonate 0 -- kl-5ts3 ethyl methanesulfonate 0 -- kl-5X1 X ray 0 -- kl-5X2 X ray 0 -- kl-5X3 X ray 0 --
Alleles Carried on Transgenic Constructs ( 1 )
For All Alleles Carried on Transgenic Constructs Show
Allele of kl-5 Class Mutagen Stocks Known lesion kl-5GD9426 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(YL)77 (Zhang and Stankiewicz, 1998) Df(YL)A11 (Hazelrigg et al., 1982) Df(YL)A32 (Hazelrigg et al., 1982) Df(YL)A39 (Hazelrigg et al., 1982) Df(YL)B4 (Hazelrigg et al., 1982) Df(YL)B12 (Hazelrigg et al., 1982) Df(YL)B14 (Hazelrigg et al., 1982) Df(YL)G21 (Hardy et al., 1984) Df(YL)G22 (Hardy et al., 1984) Df(YL)G23 (Hardy et al., 1984) Df(YL)G24 (Hardy et al., 1984) Df(YL)H121 (Bonaccorsi and Lohe, 1991) Df(YL)S7 (Gatti and Pimpinelli, 1983, Gepner and Hays, 1993) Df(YL)S8 (Gatti and Pimpinelli, 1983) Df(YL)S9 (Gatti and Pimpinelli, 1983, Gepner and Hays, 1993) Df(YL)S10 (Gatti and Pimpinelli, 1983) Df(YL)S11 (Gatti and Pimpinelli, 1983) Df(YL)S13 (Gatti and Pimpinelli, 1983) Df(YL)S14 (Gatti and Pimpinelli, 1983) Df(YL)S21 (Gatti and Pimpinelli, 1983) In(YL)B2 (Gatti and Pimpinelli, 1983) In(YL)B3 (Gatti and Pimpinelli, 1983) T(1;Y)L2   T(1;Y)N18   T(1;Y)N20   T(1;Y)R21   T(1;Y)R29   T(1;Y)R36   T(1;Y)S20   T(1;Y)S25   T(1;Y)T2   T(1;Y)T4   T(1;Y)T11   T(Y;2)A24 (Gatti and Pimpinelli, 1983) T(Y;2)B110 (Gatti and Pimpinelli, 1983) T(Y;2)D19 (Gatti and Pimpinelli, 1983, Bonaccorsi and Lohe, 1991) T(Y;2)G53 (Gatti and Pimpinelli, 1983) T(Y;2)G74 (Gatti and Pimpinelli, 1983, Bonaccorsi and Lohe, 1991) T(Y;2)R50 (Gatti and Pimpinelli, 1983, Bonaccorsi and Lohe, 1991) T(Y;3)A121 (Gatti and Pimpinelli, 1983) T(Y;3)B130 (Gatti and Pimpinelli, 1983) T(Y;3)D100 (Gatti and Pimpinelli, 1983) T(Y;3)D107 (Gatti and Pimpinelli, 1983) T(Y;3)G42 (Gatti and Pimpinelli, 1983, Bonaccorsi and Lohe, 1991) T(Y;3)G122 (Gatti and Pimpinelli, 1983) T(Y;3)J94 (Gatti and Pimpinelli, 1983) T(Y;3)R71 (Gatti and Pimpinelli, 1983) T(Y;3)S21   Ts(1Lt;YSt)E15 (Zhang and Stankiewicz, 1998) Ts(1Lt;YSt)F12 (Zhang and Stankiewicz, 1998) Ts(1Lt;YSt)T13 (Bonaccorsi et al., 1990) Ts(1Lt;YSt)V24 (Zhang and Stankiewicz, 1998) Ts(1Lt;YSt)W27 (Zhang and Stankiewicz, 1998) Ts(1Rt;YSt)V8 (Bonaccorsi et al., 1990) Ts(1Rt;YSt)W19 (Bonaccorsi et al., 1990) Ts(YSt;4Rt)B15 (Hazelrigg et al., 1982)
Not disrupted in	C(1;Y)E12   Df(YL)A10 (Hazelrigg et al., 1982) Df(YL)A17 (Hazelrigg et al., 1982) Df(YL)A49 (Hazelrigg et al., 1982) Df(YL)S5 (Gatti and Pimpinelli, 1983) Df(YL)S6 (Gatti and Pimpinelli, 1983) Df(YL)S10 (Gepner and Hays, 1993) Df(YS)A6 (Hazelrigg et al., 1982) Df(YS)B13 (Hazelrigg et al., 1982) In(YL)S15 (Gatti and Pimpinelli, 1983) In(YL)S16L (Gatti and Pimpinelli, 1983) In(YLS)A33 (Hazelrigg et al., 1982) In(YLS)B1 (Gatti and Pimpinelli, 1983) In(YLS)S18 (Gatti and Pimpinelli, 1983) T(1;Y)V24 (Gatti and Pimpinelli, 1983) T(Y;2)A169 (Gatti and Pimpinelli, 1983) T(Y;2)B3 (Hazelrigg et al., 1982) T(Y;2)B13 (Hazelrigg et al., 1982) T(Y;2)B92 (Gatti and Pimpinelli, 1983) T(Y;2)B242 (Gatti and Pimpinelli, 1983) T(Y;2)J146 (Gatti and Pimpinelli, 1983) T(Y;2)P57 (Gatti and Pimpinelli, 1983) T(Y;2)R155 (Gatti and Pimpinelli, 1983) T(Y;3)A23 (Gatti and Pimpinelli, 1983) T(Y;3)A34 (Hazelrigg et al., 1982) T(Y;3)A112 (Gatti and Pimpinelli, 1983) T(Y;3)B115 (Gatti and Pimpinelli, 1983) T(Y;3)B223 (Gatti and Pimpinelli, 1983) T(Y;3)B229 (Gatti and Pimpinelli, 1983) T(Y;3)S20 (Gatti and Pimpinelli, 1983) Ts(1Lt;YLt)V8 (Zhang and Stankiewicz, 1998) Ts(1Lt;YLt)W19 (Zhang and Stankiewicz, 1998) Ts(1Lt;YSt)E15 (Gepner and Hays, 1993) Ts(1Lt;YSt)F12 (Gepner and Hays, 1993) Ts(1Lt;YSt)V24 (Gepner and Hays, 1993) Ts(1Lt;YSt)W27 (Gepner and Hays, 1993) Ts(1Rt;YLt)V24 (Bonaccorsi et al., 1990) Ts(YLt;3Lt)W27 (Bonaccorsi et al., 1990) Ts(YLt;3Rt)A34 (Hazelrigg et al., 1982) Ts(YLt;4Lt)B15 (Hazelrigg et al., 1982)
Duplicated in	Ts(1Rt;YLt)E15 (Gepner and Hays, 1993) Ts(1Rt;YLt)F12 (Gepner and Hays, 1993) Ts(1Rt;YLt)V24 (Gepner and Hays, 1993) Ts(YLt;3Lt)W27 (Gepner and Hays, 1993)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data characterization construct P{GD9426} NA
Insertions
	Type of insertions Name Expression data insertion of enhancer trap P{PZ}kl-516 No
Gene Ontology: Function, Process & Cellular Component ( 9 unique terms )
Terms Based on Experimental Evidence ( 0 terms )
Terms Based on Predictions or Assertions ( 9 terms )
Molecular Function
CV term Evidence References
ATPase activity inferred from electronic annotation with InterPro:IPR011704 (FlyBase Curators et al., 2004-) ATPase activity, coupled inferred from sequence or structural similarity (Goldstein and Gunawardena, 2000) ATP binding inferred from electronic annotation with InterPro:IPR011704 (FlyBase Curators et al., 2004-) microtubule binding inferred from sequence or structural similarity (Kurek et al., 1999) inferred from sequence or structural similarity with Saccharomyces DYN1 (FlyBase, 1992-) microtubule motor activity inferred from electronic annotation with InterPro:IPR004273 (FlyBase Curators et al., 2004-) motor activity inferred from sequence or structural similarity (Kurek et al., 1999) inferred from sequence or structural similarity with Saccharomyces DYN1 (FlyBase, 1992-)
Biological Process
CV term Evidence References
microtubule-based movement inferred from electronic annotation with InterPro:IPR004273 (FlyBase Curators et al., 2004-) inferred from sequence or structural similarity (Goldstein and Gunawardena, 2000)
Cellular Component
CV term Evidence References
axonemal dynein complex inferred from sequence or structural similarity (Kurek et al., 1999, Goldstein and Gunawardena, 2000) inferred from sequence or structural similarity with Saccharomyces DYN1 (FlyBase, 1992-) microtubule associated complex inferred from sequence or structural similarity with Saccharomyces DYN1 (FlyBase, 1992-) non-traceable author statement (Gepner, 1993)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions kl-5 allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 78300 DroID - A comprehensive database of gene and protein interactions. • FBgn0001315 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 3355175
Orthologs
Genome-wide drosophilid orthologs
Curated drosophilid orthologs	Dant\kl-5   Dark\kl-5   Darw\kl-5   Dawd\kl-5   Dcai\kl-5   Deoh\Dhc7Y (Kurek et al., 2000) Dhyd\thread (Kurek et al., 1998) Dmau\Dhc-Yh3 (Zurovcova and Eanes, 1999) Dngo\kl-5   Dpse\kl-5 (Carvalho et al., 2006, Carvalho and Clark, 2005) Dsal\kl-5 (Carvalho et al., 2006) Dsan\Dhc-Yh3 (Llopart et al., 2005) Dsec\Dhc-Yh3 (Zurovcova and Eanes, 1999) Dsig\kl-5   Dsii\kl-5   Dsim\Dhc-Yh3 (Zurovcova and Eanes, 1999) Dwil\kl-5 (Carvalho et al., 2006) Dyak\Dhc-Yh3 (Zurovcova and Eanes, 1999)
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • GL50803_103059 Giardia lamblia (flagellated protozoan) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • FBgn0111982
Stocks & Reagents
Stocks Listed in FlyBase ( 1 )
Bloomington	2763 T(Y;2)R50, kl-5R50 BS, y+/SM1; C(1)RM, y1/C(1;Y)1, y1
Genomic Clones ( 0 )
cDNA Clones ( 1 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones
Other clones	DMG5-chrYHet.1.001.pred.a
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0001315 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 3355175
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: CG12573 CG17616 (FlyBase Genome Annotators, 2002-2003) Source for merge of: kl-5 Dhc-Yh3 (Reugels et al., 1999)
Additional comments	Annotations CG12573 and CG17616 merged as CG40444 in release 3 of the genome annotation. (FlyBase Genome Annotators, 2002-2003) Williamson (1972) reported 26 non-complementing and nine complementing alleles induced by EMS.
Other Comments
	dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003) Identification: PCR screen for Dynein heavy chain genes. (Kurek et al., 1998) kl-5 has been cloned and characterised. (Rasmusson et al., 1994) kl-5 gene locus encodes a dynein heavy chain, the dynein heavy chain sequences are localised to the kl-5 region of the Y chromosome. These results provide evidence that at least one of the Y chromosome fertility regions encodes a polypeptide required for spermatogenesis. (Gepner and Hays, 1993) Primary spermatocyte nuclei exhibit three giant lampbrush-like loops formed by the kl-5, kl-3 and ks-1 Y chromosome fertility factors. (Pisano et al., 1993) AAGAC satellite DNA repeats which map to the kl-5 and ks-1 loop forming regions are abundantly transcribed in primary spermatocytes. These transcripts are detected at all stages of development of these two loops, do not appear to migrate to the cytoplasm and are degraded when the loops disintegrate during the first meiotic prophase. (Bonaccorsi et al., 1990) Males mutant for kl-5 fail to assemble the outer dynein arms associated with the A tubules of the peripheral doublets of the sperm tail axoneme (Hardy, Tokuyasu, and Lindsley, 1981). Such males also fail to produce kl-5-specific 300-325 kilodalton sperm polypeptide considered to be a component of the outer arms (Goldstein, Hardy and Lindsley, 1982). kl-5 mutants exhibit very low level of fertility at 25oC but not at 18oC and in young but not older males (Kennison, 1983). Deficiencies for kl-5 completely sterile and in addition to the mutant phenotype they fail to produce aggregates of tubuli normally seen in thin sections of primary spermatocyte nuclei (Hardy et al., 1981). Within the kl-5 fertility region of the spermatocyte nucleus, a loop-forming site has been demonstrated by its reaction with the monoclonal antibody S5 (Bonaccorsi et al., 1988). These loops are stained with the protein-specific dye CBB, but are not visible in living preparations.
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_001015499 RefSeq (Proteins) • NP_001015499 DDBJ / EMBL / GenBank • AAA52099 AAF21041 AAF21324 AAF21325 AAF21326 AAF21327 AAF21328 AAF21329 AAF21330 AAF21331 AAF21332 AAF21333 AAF21334 AF136243 AF136244 AF136245 AF136246 AF136247 AF136248 AF136249 AF136250 AF136251 AF136252 AF136253 AF210453 EAL24525 L23199 Entrez Gene - A searchable database of RefSeq genes. • 3355175 UniProtKB/TrEMBL • Q5LJN5 Q9TW74 Q9U3Y5
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Dynein heavy chain (IPR004273) ATPase, dynein-related, AAA domain (IPR011704) Dynein heavy chain, domain-1 (IPR013594) Dynein heavy chain, domain-2 (IPR013602) ATPase, AAA+ type, core (IPR003593) Dynein heavy chain, P-loop containing D4 domain (IPR024317) Dynein heavy chain, coiled coil stalk (IPR024743)
Linkouts
	BioGRID - A database of protein and genetic interactions • 78300 DroID - A comprehensive database of gene and protein interactions. • FBgn0001315 DRSC - Results from RNAi screens. • FBgn0001315 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0001315 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG40444-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0001315 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 3355175 InParanoid A subset of ortholog calls from InParanoid. • GL50803_103059 Giardia lamblia (flagellated protozoan) InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 3355175 modMine - Data generated by the modENCODE project. • FBgn0001315 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • FBgn0111982
Synonyms & Secondary IDs ( 15 )
Reported As
Symbol Synonym	CG12573 (Misra, 2001.8.16, ) CG17616 (Goldstein and Gunawardena, 2000, Przewloka et al., 2007) CG40444 (Drosophila Heterochromatin Genome Project, 2007) Dhc (Starr et al., 1998) Dhc-Yh3 (Kurek et al., 2000, Zurovcova and Eanes, 1999, Reugels et al., 1999, Kurek et al., 1999, Kurek et al., 1999, Gepner and Hays, 1993, ) DhcYh3 (Kurek et al., 1998, Rasmusson et al., 1994) kl-5 (Koerich et al., 2007, Carvalho and Clark, 2005, Larracuente and Clark, 2008, Pisano et al., 1993, Diaz-Castillo and Golic, 2007, Koerich et al., 2008, Piergentili, 2006, Ding et al., 2010, Wasbrough et al., 2010, Wasbrough et al., 2010) kl5 (Zhang et al., 1998) Kl-5 (Lemos et al., 2008) Lms4 (Hazelrigg et al., 1982, ) unnamed (Goldstein and Gunawardena, 2000)
Name Synonym	Dynein heavy chain in Yh3   dynein-related heavy chain (Gepner, 1993) male fertility factor kl5 (Lott et al., 2011, Wasbrough et al., 2010) Y-linked flagellar dynein (Charlesworth and Charlesworth, 2000)
Secondary FlyBase IDs
	FBan0040444 FBgn0010386 FBgn0040029 FBgn0058444
References ( 78 )
Generate a list of	All references relating to this gene ( 78 )
List References by type	Research paper  ( 41 ) Supplementary material  ( 6 ) Review  ( 11 ) Personal communication to FlyBase  ( 5 ) Abstract  ( 8 ) FlyBase analysis  ( 2 ) DNA/RNA sequence record  ( 1 ) Automatic genome annotation  ( 1 ) Curated genome annotation  ( 1 ) Book  ( 2 )
Recent research papers ( 3 )
	Lott et al., 2011, PLoS Biol. 9(2): e1000590 Noncanonical Compensation of Zygotic X Transcription in Early Drosophila melanogaster Development Revealed through Single-Embryo RNA-Seq. [FBrf0213135] Ding et al., 2010, PLoS Genet. 6(12): e1001255 A young Drosophila duplicate gene plays essential roles in spermatogenesis by regulating several y-linked male fertility genes. [FBrf0212639] Wasbrough et al., 2010, J. Proteomics 73(11): 2171--2185 The Drosophila melanogaster sperm proteome-II (DmSP-II). [FBrf0211978] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...