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Gene Dmel\klar

General Information
Symbol	Dmel\klar	Species	D. melanogaster
Name	klarsicht	Annotation symbol	CG17046
Feature type	protein_coding_gene	FlyBase ID	FBgn0001316
Gene Model Status	Current	Stock availability	40 publicly available
Also Known As	kls, marb
Genomic Location
Chromosome (arm)	3L	Recombination map	3-0.0
Cytogenetic map	61C1-61C3	Sequence location	3L:434,089..540,589 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene klarsicht is referred to in FlyBase by the symbol Dmel\klar (CG17046, FBgn0001316). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: protein binding. There is experimental evidence for 12 unique biological process terms, many of which group under: localization; single-organism developmental process; cellular localization; sensory organ development; locomotion; flight; compound eye photoreceptor development; cellular component organization or biogenesis; biological regulation; larval visceral muscle development; open tracheal system development; organic substance transport; membrane organization; cellular component movement. 89 alleles are reported. The phenotypes of these alleles are annotated with: external compound sense organ; organ system subdivision; peripheral nervous system; organ system; cell part; histaminergic neuron; imaginal precursor; cytoplasmic part; embryonic foregut; rhabdomere; tracheal system; non-connected developing system; lipid particle. It has 7 annotated transcripts and 7 annotated polypeptides. Protein features are: Klarsicht/ANC-1/syne-1 homology. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 12-24 hour embryonic stages, during late pupal stages. Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult tissues/organs expressed at moderate levels. Expression at high levels in the following post-embryonic organs or tissues: larval central nervous system. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male accessory gland, larval/adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1628155_at partially aligns to an exonic region of only a subset of 3 of the 4 FlyBase-annotated transcript isoforms of klar: 13 out of 14 probes fall into this exonic region. Gene sequence location is 3L:434089..540589.
User Contributed Data
	Beta version, feedback welcome
External Summaries
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	kls: klarsicht Maternal effect mutant; without effect on viability or fertility of homozygotes. Homozygous females produce eggs with clear distinction between yolk and germ layers. Facilitates in vivo observation of developmental processes.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03	Alleles klar[MI06820]
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 61C1-61C3   Limits computationally determined from genome sequence between P{PZ}trh10512 and P{EP}EP3104EP3104
Experimentally Determined Cytological Location
Cytogenetic map Notes References 61C-61C     (Mosley-Bishop, 1999.6.6) 61C4-61C7   (determined by in situ hybridisation)   (Fischer, 1993) 61C-61C   Location based on deficiency mapping.   (Welte et al., 1998)
Experimentally Determined Recombination Data
Location	3-0.0
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\klar for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.46 Gene model reviewed during 5.48 Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters. Annotated transcripts do not represent all supported alternative splices within 5' UTR. Low-frequency RNA-Seq exon junction(s) not annotated. Gene model includes transcripts encoding non-overlapping portions of the full CDS.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) klar-RA FBtr0072565  NM_079149   8681   2262 klar-RB FBtr0110818  NM_001043111   8240   2045 klar-RC FBtr0112807  NM_001103992   3032   567 klar-RD FBtr0112808  NM_001103993   6842   1823 klar-RE FBtr0333850  NM_001274282   8617   2272 klar-RF FBtr0333851  NM_001274283   7507   1636 klar-RG FBtr0333852  NM_001274284   4934   1180
Additional Transcript Data & Comments
Reported size (kB)	8.5, 8.0 (northern blot) (Mosley-Bishop et al., 1999)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein klar-PA   FBpp0072464   245.2   2262   5.90     NP_523873   AAF47389 klar-PB   FBpp0110115   221.3   2045   5.75     NP_001036576   ABI31227 klar-PC   FBpp0111719   62.1   567   5.44     NP_001097462   ABW08427 klar-PD   FBpp0111720   197.4   1823   6.27     NP_001097463   ABW08428 klar-PE   FBpp0305983   246.3   2272   6.00     NP_001261211   AGB93906 klar-PF   FBpp0305984   176.9   1636   6.81     NP_001261212   AGB93907 klar-PG   FBpp0305985   128.9   1180   6.40     NP_001261213   AGB93908
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Klarsicht/ANC-1/syne-1 homology (IPR012315)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA264557   AA439085   AA695221   AA696845   AA698941   AA801900   AA820623   AA941751   AE014296 AAF47389   AE014296 ABI31227   AE014296 ABW08427   AE014296 ABW08428   AF157066 AAD43129   AE014296 AGB93906   AE014296 AGB93907   AE014296 AGB93908   AI062542   AI108430   AI109286   AI237928   AI238621   AI402746   AI403261   AI455477   AI511961   AI531209   AI543472   AW941315   AW941666   AX094211   AY060946 AAL28494   BF497319   BG633195   BG638457   BG639096   BG639535   BG640411   BG641183   BI141919   BI235671   BI352618   BI353126   BI369734   BI483244   BI486445   BI569672   BI633788   BP546468   BP556157   BT010042   BT120378   CL528101   CL705938   CL868601   CO156176   CO156199   CO179045   CO180186   CO186272   CO269099   CO273978   CO274314   CO281314   CO282228   CO285132   CO287074   CO287078   CO288179   CO289425   CO303406   CO307266   CO307335   CO319574   CO326119   CO331797   CO332637   CO336594   CO336771   CO337446   CO339661   CO342148   CZ466919   CZ467354   CZ467385   CZ467386   CZ468396   CZ468812   CZ474796   CZ481002   CZ481161   CZ481162   CZ481831   CZ484508   CZ488551   CZ488552   CZ488893   CZ490069   CZ490086   CZ490400   DN154499   EC062900   EC063813   EC072033   EC079278   EC081670   EC196184   EC196943   EC203578   EC205211   EC208363   EC210916   EC214470   EC214882   EC218674   EC218889   EC228710   EC231288   EC232596   EC233778   EC240496   EC241944   EC243078   EC250352   EC250561   EC256522   EC257195   EC263692   EC266031   EC266118   EC266654   EC266891   EC267859   EL872364   EL887011   ES692160   EV576520   EV587881   EV590255   EV591996
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q9W0Q4 A8JNH2 Q7YU15 A8JNH1 Q95S58 Q9Y0E4
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References regulatory region FBsf0000435051 3L:522,945..524,581 evidence=Reporter construct (in vivo) (Busser et al., 2012, Halfon, 2012.6.19)
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage | late   adult salivary gland primordium | dorsal & posterior   (Myat and Andrew, 2002) third instar larval stage   morphogenetic furrow | posterior to   (Mosley-Bishop et al., 1999) northern blot Stage Tissue/Position (including subcellular localization) Reference third instar larval stage   eye disc   (Mosley-Bishop et al., 1999)
Additional Descriptive Data
	Prior to and during invagination of the salivary gland placodes klar transcripts were elevated in the dorsal and posterior cells. (Myat and Andrew, 2002)
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
No Assay Recorded Stage Tissue/Position (including subcellular localization) Reference third instar larval stage   photoreceptor   (Mosley-Bishop et al., 1999)
Additional Descriptive Data
	Epitope-tagged klar protein is perinuclear in developing photoreceptors. (Mosley-Bishop et al., 1999)
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
cytoplasm inferred from direct assay (Kracklauer et al., 2007) colocalizes_with cytoplasmic microtubule inferred from direct assay (Kracklauer et al., 2007) cytoplasmic microtubule inferred from direct assay (Elhanany-Tamir et al., 2012) colocalizes_with lipid particle inferred from direct assay (Guo et al., 2005) lipid particle inferred from direct assay (Cermelli et al., 2006) nuclear envelope inferred from direct assay (Patterson et al., 2004) colocalizes_with nuclear envelope inferred from direct assay (Guo et al., 2005) perinuclear region of cytoplasm inferred from direct assay (Mosley-Bishop et al., 1999, Kracklauer et al., 2007)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult tissues/organs expressed at moderate levels. Expression at high levels in the following post-embryonic organs or tissues: larval central nervous system. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male accessory gland, larval/adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    649.925 Larval Midgut    372.9 Larval Hindgut    352.9 Larval Malpighian Tubules    209.9 Larval Fat Body    139.7 Larval Salivary Gland    200 Larval Trachea    477.8 Larval Carcass    309.225 Adult Head    188.8 Adult Eye    307.625 Adult Brain    262.7 Adult Thoracic-Abdominal Ganglion    269.5 Adult Crop    274.1 Adult Midgut    384.8 Adult Hindgut    341.5 Adult Malpighian Tubules    205.5 Adult Fat Body    176.2 Adult Salivary Gland    149.1 Adult Heart    251.475 Adult VirginFemale Spermatheca    253.2 Adult InseminatedFemale Spermatheca    271.3 Adult Ovary    232.3 Adult Testis    99.5 Adult Male Accessory Gland    151.2 Adult Carcass    217 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  (649.925) Larval Midgut    372.9 Larval Hindgut    352.9 Larval Malpighian Tubules    209.9 Larval Fat Body    139.7 Larval Salivary Gland    200 Larval Trachea    477.8 Larval Carcass    309.225 Adult Head    188.8 Adult Eye    307.625 Adult Brain    262.7 Adult Thoracic-Abdominal Ganglion    269.5 Adult Crop    274.1 Adult Midgut    384.8 Adult Hindgut    341.5 Adult Malpighian Tubules    205.5 Adult Fat Body    176.2 Adult Salivary Gland    149.1 Adult Heart    251.475 Adult VirginFemale Spermatheca    253.2 Adult InseminatedFemale Spermatheca    271.3 Adult Ovary    232.3 Adult Testis    99.5 Adult Male Accessory Gland    151.2 Adult Carcass    217 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    649.925 Larval Midgut    372.9 Larval Hindgut    352.9 Larval Malpighian Tubules    209.9 Larval Fat Body    139.7 Larval Salivary Gland    200 Larval Trachea    477.8 Larval Carcass    309.225 Adult Head    188.8 Adult Eye    307.625 Adult Brain    262.7 Adult Thoracic-Abdominal Ganglion    269.5 Adult Crop    274.1 Adult Midgut    384.8 Adult Hindgut    341.5 Adult Malpighian Tubules    205.5 Adult Fat Body    176.2 Adult Salivary Gland    149.1 Adult Heart    251.475 Adult VirginFemale Spermatheca    253.2 Adult InseminatedFemale Spermatheca    271.3 Adult Ovary    232.3 Adult Testis    99.5 Adult Male Accessory Gland    151.2 Adult Carcass    217 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    649.925 Larval Midgut    372.9 Larval Hindgut    352.9 Larval Malpighian Tubules    209.9 Larval Fat Body    139.7 Larval Salivary Gland    200 Larval Trachea    477.8 Larval Carcass    309.225 Adult Head    188.8 Adult Eye    307.625 Adult Brain    262.7 Adult Thoracic-Abdominal Ganglion    269.5 Adult Crop    274.1 Adult Midgut    384.8 Adult Hindgut    341.5 Adult Malpighian Tubules    205.5 Adult Fat Body    176.2 Adult Salivary Gland    149.1 Adult Heart    251.475 Adult VirginFemale Spermatheca    253.2 Adult InseminatedFemale Spermatheca    271.3 Adult Ovary    232.3 Adult Testis    99.5 Adult Male Accessory Gland    151.2 Adult Carcass    217 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    649.925 Larval Midgut    372.9 Larval Hindgut    352.9 Larval Malpighian Tubules    209.9 Larval Fat Body    139.7 Larval Salivary Gland    200 Larval Trachea    477.8 Larval Carcass    309.225 Adult Head    188.8 Adult Eye    307.625 Adult Brain    262.7 Adult Thoracic-Abdominal Ganglion    269.5 Adult Crop    274.1 Adult Midgut    384.8 Adult Hindgut    341.5 Adult Malpighian Tubules    205.5 Adult Fat Body    176.2 Adult Salivary Gland    149.1 Adult Heart    251.475 Adult VirginFemale Spermatheca    253.2 Adult InseminatedFemale Spermatheca    271.3 Adult Ovary    232.3 Adult Testis    99.5 Adult Male Accessory Gland    151.2 Adult Carcass    217 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  649.925 Larval Midgut    372.9 Larval Hindgut    352.9 Larval Malpighian Tubules    209.9 Larval Fat Body    139.7 Larval Salivary Gland    200 Larval Trachea    477.8 Larval Carcass    309.225 Adult Head    188.8 Adult Eye    307.625 Adult Brain    262.7 Adult Thoracic-Abdominal Ganglion    269.5 Adult Crop    274.1 Adult Midgut    384.8 Adult Hindgut    341.5 Adult Malpighian Tubules    205.5 Adult Fat Body    176.2 Adult Salivary Gland    149.1 Adult Heart    251.475 Adult VirginFemale Spermatheca    253.2 Adult InseminatedFemale Spermatheca    271.3 Adult Ovary    232.3 Adult Testis    99.5 Adult Male Accessory Gland    151.2 Adult Carcass    217 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    649.925 Larval Midgut    372.9 Larval Hindgut    352.9 Larval Malpighian Tubules    209.9 Larval Fat Body    139.7 Larval Salivary Gland    200 Larval Trachea    477.8 Larval Carcass    309.225 Adult Head    188.8 Adult Eye    307.625 Adult Brain    262.7 Adult Thoracic-Abdominal Ganglion    269.5 Adult Crop    274.1 Adult Midgut    384.8 Adult Hindgut    341.5 Adult Malpighian Tubules    205.5 Adult Fat Body    176.2 Adult Salivary Gland    149.1 Adult Heart    251.475 Adult VirginFemale Spermatheca    253.2 Adult InseminatedFemale Spermatheca    271.3 Adult Ovary    232.3 Adult Testis    99.5 Adult Male Accessory Gland    151.2 Adult Carcass    217 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    649.925 Larval Midgut    372.9 Larval Hindgut    352.9 Larval Malpighian Tubules    209.9 Larval Fat Body    139.7 Larval Salivary Gland    200 Larval Trachea    477.8 Larval Carcass    309.225 Adult Head    188.8 Adult Eye    307.625 Adult Brain    262.7 Adult Thoracic-Abdominal Ganglion    269.5 Adult Crop    274.1 Adult Midgut    384.8 Adult Hindgut    341.5 Adult Malpighian Tubules    205.5 Adult Fat Body    176.2 Adult Salivary Gland    149.1 Adult Heart    251.475 Adult VirginFemale Spermatheca    253.2 Adult InseminatedFemale Spermatheca    271.3 Adult Ovary    232.3 Adult Testis    99.5 Adult Male Accessory Gland    151.2 Adult Carcass    217 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    188.8   NA  Eye    307.625   NA  Brain    262.7   649.925  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    269.5   NA  Crop    274.1   372.9  Midgut    384.8   352.9  Hindgut    341.5   209.9  Malpighian Tubules    205.5   139.7  Fat Body    176.2   200  Salivary Gland    149.1   NA  Heart    251.475   477.8  Trachea    NA   NA  VirginFemale Spermatheca    253.2   NA  InseminatedFemale Spermatheca    271.3   NA  Ovary    232.3   NA  Testis    99.5   NA  Male Accessory Gland    151.2   309.225  Carcass    217 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    33 central nervous system, larvae L3    28 central nervous system, pupae P8    18 head, virgin 1-day female    8 head, virgin 4-day female    8 head, virgin 20-day female    7 head, mated 1-day female    8 head, mated 4-day female    7 head, mated 20-day female    7 head, mated 1-day male    12 head, mated 4-day male    11 head, mated 20-day male    11 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    4 digestive system, larvae L3 wandering    9 digestive system, 1-day adult    12 digestive system, 4-day adult    15 digestive system, 20-day adult    14 fat body, larvae L3 wandering    2 fat body, white prepupae    8 fat body, pupae P8    21 carcass, larvae L3 wandering    23 carcass, 1-day adult    8 carcass, 4-day adult    8 carcass, 20-day adult    7 ovary, virgin 4-day female    36 ovary, mated 4-day female    34 testis, mated 4-day male    4 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (33) central nervous system, larvae L3  (28) central nervous system, pupae P8    18 head, virgin 1-day female    8 head, virgin 4-day female    8 head, virgin 20-day female    7 head, mated 1-day female    8 head, mated 4-day female    7 head, mated 20-day female    7 head, mated 1-day male    12 head, mated 4-day male    11 head, mated 20-day male    11 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    4 digestive system, larvae L3 wandering    9 digestive system, 1-day adult    12 digestive system, 4-day adult    15 digestive system, 20-day adult    14 fat body, larvae L3 wandering    2 fat body, white prepupae    8 fat body, pupae P8    21 carcass, larvae L3 wandering    23 carcass, 1-day adult    8 carcass, 4-day adult    8 carcass, 20-day adult    7 ovary, virgin 4-day female  (36) ovary, mated 4-day female  (34) testis, mated 4-day male    4 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    33 central nervous system, larvae L3    28 central nervous system, pupae P8    18 head, virgin 1-day female    8 head, virgin 4-day female    8 head, virgin 20-day female    7 head, mated 1-day female    8 head, mated 4-day female    7 head, mated 20-day female    7 head, mated 1-day male    12 head, mated 4-day male    11 head, mated 20-day male    11 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    4 digestive system, larvae L3 wandering    9 digestive system, 1-day adult    12 digestive system, 4-day adult    15 digestive system, 20-day adult    14 fat body, larvae L3 wandering    2 fat body, white prepupae    8 fat body, pupae P8    21 carcass, larvae L3 wandering    23 carcass, 1-day adult    8 carcass, 4-day adult    8 carcass, 20-day adult    7 ovary, virgin 4-day female    36 ovary, mated 4-day female    34 testis, mated 4-day male    4 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    33 central nervous system, larvae L3    28 central nervous system, pupae P8    18 head, virgin 1-day female    8 head, virgin 4-day female    8 head, virgin 20-day female    7 head, mated 1-day female    8 head, mated 4-day female    7 head, mated 20-day female    7 head, mated 1-day male    12 head, mated 4-day male    11 head, mated 20-day male    11 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    4 digestive system, larvae L3 wandering    9 digestive system, 1-day adult    12 digestive system, 4-day adult    15 digestive system, 20-day adult    14 fat body, larvae L3 wandering    2 fat body, white prepupae    8 fat body, pupae P8    21 carcass, larvae L3 wandering    23 carcass, 1-day adult    8 carcass, 4-day adult    8 carcass, 20-day adult    7 ovary, virgin 4-day female    36 ovary, mated 4-day female    34 testis, mated 4-day male    4 accessory gland, mated 4-day male    4 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    33 central nervous system, larvae L3    28 central nervous system, pupae P8    18 head, virgin 1-day female    8 head, virgin 4-day female    8 head, virgin 20-day female    7 head, mated 1-day female    8 head, mated 4-day female    7 head, mated 20-day female    7 head, mated 1-day male    12 head, mated 4-day male    11 head, mated 20-day male    11 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    4 digestive system, larvae L3 wandering    9 digestive system, 1-day adult    12 digestive system, 4-day adult    15 digestive system, 20-day adult    14 fat body, larvae L3 wandering    2 fat body, white prepupae    8 fat body, pupae P8    21 carcass, larvae L3 wandering    23 carcass, 1-day adult    8 carcass, 4-day adult    8 carcass, 20-day adult    7 ovary, virgin 4-day female    36 ovary, mated 4-day female    34 testis, mated 4-day male    4 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (33) central nervous system, larvae L3  28 central nervous system, pupae P8    18 head, virgin 1-day female    8 head, virgin 4-day female    8 head, virgin 20-day female    7 head, mated 1-day female    8 head, mated 4-day female    7 head, mated 20-day female    7 head, mated 1-day male    12 head, mated 4-day male    11 head, mated 20-day male    11 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    4 digestive system, larvae L3 wandering    9 digestive system, 1-day adult    12 digestive system, 4-day adult    15 digestive system, 20-day adult    14 fat body, larvae L3 wandering    2 fat body, white prepupae    8 fat body, pupae P8    21 carcass, larvae L3 wandering    23 carcass, 1-day adult    8 carcass, 4-day adult    8 carcass, 20-day adult    7 ovary, virgin 4-day female  (36) ovary, mated 4-day female  (34) testis, mated 4-day male    4 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    33 central nervous system, larvae L3    28 central nervous system, pupae P8    18 head, virgin 1-day female    8 head, virgin 4-day female    8 head, virgin 20-day female    7 head, mated 1-day female    8 head, mated 4-day female    7 head, mated 20-day female    7 head, mated 1-day male    12 head, mated 4-day male    11 head, mated 20-day male    11 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    4 digestive system, larvae L3 wandering    9 digestive system, 1-day adult    12 digestive system, 4-day adult    15 digestive system, 20-day adult    14 fat body, larvae L3 wandering    2 fat body, white prepupae    8 fat body, pupae P8    21 carcass, larvae L3 wandering    23 carcass, 1-day adult    8 carcass, 4-day adult    8 carcass, 20-day adult    7 ovary, virgin 4-day female    36 ovary, mated 4-day female    34 testis, mated 4-day male    4 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    33 central nervous system, larvae L3    28 central nervous system, pupae P8    18 head, virgin 1-day female    8 head, virgin 4-day female    8 head, virgin 20-day female    7 head, mated 1-day female    8 head, mated 4-day female    7 head, mated 20-day female    7 head, mated 1-day male    12 head, mated 4-day male    11 head, mated 20-day male    11 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    4 digestive system, larvae L3 wandering    9 digestive system, 1-day adult    12 digestive system, 4-day adult    15 digestive system, 20-day adult    14 fat body, larvae L3 wandering    2 fat body, white prepupae    8 fat body, pupae P8    21 carcass, larvae L3 wandering    23 carcass, 1-day adult    8 carcass, 4-day adult    8 carcass, 20-day adult    7 ovary, virgin 4-day female    36 ovary, mated 4-day female    34 testis, mated 4-day male    4 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 12-24 hour embryonic stages, during late pupal stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    31 embryo 02-04hr    30 embryo 04-06hr    32 embryo 06-08hr    36 embryo 08-10hr    37 embryo 10-12hr    38 embryo 12-14hr    43 embryo 14-16hr    52 embryo 16-18hr    48 embryo 18-20hr    50 embryo 20-22hr    38 embryo 22-24hr    35 larva L1    15 larva L2    12 larva L3 12hr old    10 larva L3 puffstage 1-2    10 larva L3 puffstage 3-6    18 larva L3 puffstage 7-9    21 white prepupae new    27 white prepupae 12hr    26 white prepupae 24hr    40 pupae 2d postWPP    63 pupae 3d postWPP    23 pupae 4d postWPP    25 adult male 01day    15 adult male 05day    15 adult male 30day    13 adult female 01day    33 adult female 05day    42 adult female 30day    39 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (31) embryo 02-04hr  (30) embryo 04-06hr  (32) embryo 06-08hr  (36) embryo 08-10hr  (37) embryo 10-12hr  (38) embryo 12-14hr  (43) embryo 14-16hr  (52) embryo 16-18hr  (48) embryo 18-20hr  (50) embryo 20-22hr  (38) embryo 22-24hr  (35) larva L1    15 larva L2    12 larva L3 12hr old    10 larva L3 puffstage 1-2    10 larva L3 puffstage 3-6    18 larva L3 puffstage 7-9    21 white prepupae new  (27) white prepupae 12hr  26 white prepupae 24hr  (40) pupae 2d postWPP  (63) pupae 3d postWPP    23 pupae 4d postWPP    25 adult male 01day    15 adult male 05day    15 adult male 30day    13 adult female 01day  (33) adult female 05day  (42) adult female 30day  (39) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    31 embryo 02-04hr    30 embryo 04-06hr    32 embryo 06-08hr    36 embryo 08-10hr    37 embryo 10-12hr    38 embryo 12-14hr    43 embryo 14-16hr    52 embryo 16-18hr    48 embryo 18-20hr    50 embryo 20-22hr    38 embryo 22-24hr    35 larva L1    15 larva L2    12 larva L3 12hr old    10 larva L3 puffstage 1-2    10 larva L3 puffstage 3-6    18 larva L3 puffstage 7-9    21 white prepupae new    27 white prepupae 12hr    26 white prepupae 24hr    40 pupae 2d postWPP    63 pupae 3d postWPP    23 pupae 4d postWPP    25 adult male 01day    15 adult male 05day    15 adult male 30day    13 adult female 01day    33 adult female 05day    42 adult female 30day    39 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    31 embryo 02-04hr    30 embryo 04-06hr    32 embryo 06-08hr    36 embryo 08-10hr    37 embryo 10-12hr    38 embryo 12-14hr    43 embryo 14-16hr    52 embryo 16-18hr    48 embryo 18-20hr    50 embryo 20-22hr    38 embryo 22-24hr    35 larva L1    15 larva L2    12 larva L3 12hr old    10 larva L3 puffstage 1-2    10 larva L3 puffstage 3-6    18 larva L3 puffstage 7-9    21 white prepupae new    27 white prepupae 12hr    26 white prepupae 24hr    40 pupae 2d postWPP    63 pupae 3d postWPP    23 pupae 4d postWPP    25 adult male 01day    15 adult male 05day    15 adult male 30day    13 adult female 01day    33 adult female 05day    42 adult female 30day    39 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    31 embryo 02-04hr    30 embryo 04-06hr    32 embryo 06-08hr    36 embryo 08-10hr    37 embryo 10-12hr    38 embryo 12-14hr    43 embryo 14-16hr    52 embryo 16-18hr    48 embryo 18-20hr    50 embryo 20-22hr    38 embryo 22-24hr    35 larva L1    15 larva L2    12 larva L3 12hr old    10 larva L3 puffstage 1-2    10 larva L3 puffstage 3-6    18 larva L3 puffstage 7-9    21 white prepupae new    27 white prepupae 12hr    26 white prepupae 24hr    40 pupae 2d postWPP    63 pupae 3d postWPP    23 pupae 4d postWPP    25 adult male 01day    15 adult male 05day    15 adult male 30day    13 adult female 01day    33 adult female 05day    42 adult female 30day    39 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  31 embryo 02-04hr  30 embryo 04-06hr  32 embryo 06-08hr  (36) embryo 08-10hr  (37) embryo 10-12hr  (38) embryo 12-14hr  (43) embryo 14-16hr  (52) embryo 16-18hr  (48) embryo 18-20hr  (50) embryo 20-22hr  (38) embryo 22-24hr  (35) larva L1    15 larva L2    12 larva L3 12hr old    10 larva L3 puffstage 1-2    10 larva L3 puffstage 3-6    18 larva L3 puffstage 7-9    21 white prepupae new  27 white prepupae 12hr  26 white prepupae 24hr  (40) pupae 2d postWPP  (63) pupae 3d postWPP    23 pupae 4d postWPP    25 adult male 01day    15 adult male 05day    15 adult male 30day    13 adult female 01day  (33) adult female 05day  (42) adult female 30day  (39) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    31 embryo 02-04hr    30 embryo 04-06hr    32 embryo 06-08hr    36 embryo 08-10hr    37 embryo 10-12hr    38 embryo 12-14hr    43 embryo 14-16hr    52 embryo 16-18hr    48 embryo 18-20hr    50 embryo 20-22hr    38 embryo 22-24hr    35 larva L1    15 larva L2    12 larva L3 12hr old    10 larva L3 puffstage 1-2    10 larva L3 puffstage 3-6    18 larva L3 puffstage 7-9    21 white prepupae new    27 white prepupae 12hr    26 white prepupae 24hr    40 pupae 2d postWPP    63 pupae 3d postWPP    23 pupae 4d postWPP    25 adult male 01day    15 adult male 05day    15 adult male 30day    13 adult female 01day    33 adult female 05day    42 adult female 30day    39 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    31 embryo 02-04hr    30 embryo 04-06hr    32 embryo 06-08hr    36 embryo 08-10hr    37 embryo 10-12hr    38 embryo 12-14hr    43 embryo 14-16hr    52 embryo 16-18hr    48 embryo 18-20hr    50 embryo 20-22hr    38 embryo 22-24hr    35 larva L1    15 larva L2    12 larva L3 12hr old    10 larva L3 puffstage 1-2    10 larva L3 puffstage 3-6    18 larva L3 puffstage 7-9    21 white prepupae new    27 white prepupae 12hr    26 white prepupae 24hr    40 pupae 2d postWPP    63 pupae 3d postWPP    23 pupae 4d postWPP    25 adult male 01day    15 adult male 05day    15 adult male 30day    13 adult female 01day    33 adult female 05day    42 adult female 30day    39 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    15 Schneider line 2 Sg4    20 embryonic 1182-4H    68 embryonic GM2    52 embryonic Kc167    30 embryonic S1    32 embryonic S3    18 leg disc CME L1    21 wing disc CME-W2    23 wing disc ML-DmD8    61 wing disc ML-DmD9    74 wing disc ML-DmD16-c3    52 wing disc ML-DmD21    26 wing disc ML-DmD32    34 haltere disc ML-DmD17-c3    18 eye-antennal disc ML-DmD11    68 antennal disc ML-DmD20-c5    27 mixed discs ML-DmD4-c1    40 CNS ML-DmBG1-c1    40 CNS ML-DmBG2-c2    33 tumorous blood cells mbn2    24 ovary fGS/OSS    45 ovary OSC    23 ovary OSS    22 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    15 Schneider line 2 Sg4    20 embryonic 1182-4H  (68) embryonic GM2  (52) embryonic Kc167  (30) embryonic S1  (32) embryonic S3    18 leg disc CME L1    21 wing disc CME-W2    23 wing disc ML-DmD8  (61) wing disc ML-DmD9  (74) wing disc ML-DmD16-c3  (52) wing disc ML-DmD21  26 wing disc ML-DmD32  (34) haltere disc ML-DmD17-c3    18 eye-antennal disc ML-DmD11  (68) antennal disc ML-DmD20-c5  (27) mixed discs ML-DmD4-c1  (40) CNS ML-DmBG1-c1  (40) CNS ML-DmBG2-c2  (33) tumorous blood cells mbn2    24 ovary fGS/OSS  (45) ovary OSC    23 ovary OSS    22 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    15 Schneider line 2 Sg4    20 embryonic 1182-4H    68 embryonic GM2    52 embryonic Kc167    30 embryonic S1    32 embryonic S3    18 leg disc CME L1    21 wing disc CME-W2    23 wing disc ML-DmD8    61 wing disc ML-DmD9    74 wing disc ML-DmD16-c3    52 wing disc ML-DmD21    26 wing disc ML-DmD32    34 haltere disc ML-DmD17-c3    18 eye-antennal disc ML-DmD11    68 antennal disc ML-DmD20-c5    27 mixed discs ML-DmD4-c1    40 CNS ML-DmBG1-c1    40 CNS ML-DmBG2-c2    33 tumorous blood cells mbn2    24 ovary fGS/OSS    45 ovary OSC    23 ovary OSS    22 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    15 Schneider line 2 Sg4    20 embryonic 1182-4H    68 embryonic GM2    52 embryonic Kc167    30 embryonic S1    32 embryonic S3    18 leg disc CME L1    21 wing disc CME-W2    23 wing disc ML-DmD8    61 wing disc ML-DmD9    74 wing disc ML-DmD16-c3    52 wing disc ML-DmD21    26 wing disc ML-DmD32    34 haltere disc ML-DmD17-c3    18 eye-antennal disc ML-DmD11    68 antennal disc ML-DmD20-c5    27 mixed discs ML-DmD4-c1    40 CNS ML-DmBG1-c1    40 CNS ML-DmBG2-c2    33 tumorous blood cells mbn2    24 ovary fGS/OSS    45 ovary OSC    23 ovary OSS    22 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    15 Schneider line 2 Sg4    20 embryonic 1182-4H    68 embryonic GM2    52 embryonic Kc167    30 embryonic S1    32 embryonic S3    18 leg disc CME L1    21 wing disc CME-W2    23 wing disc ML-DmD8    61 wing disc ML-DmD9    74 wing disc ML-DmD16-c3    52 wing disc ML-DmD21    26 wing disc ML-DmD32    34 haltere disc ML-DmD17-c3    18 eye-antennal disc ML-DmD11    68 antennal disc ML-DmD20-c5    27 mixed discs ML-DmD4-c1    40 CNS ML-DmBG1-c1    40 CNS ML-DmBG2-c2    33 tumorous blood cells mbn2    24 ovary fGS/OSS    45 ovary OSC    23 ovary OSS    22 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    15 Schneider line 2 Sg4    20 embryonic 1182-4H  (68) embryonic GM2  (52) embryonic Kc167  30 embryonic S1  32 embryonic S3    18 leg disc CME L1    21 wing disc CME-W2    23 wing disc ML-DmD8  (61) wing disc ML-DmD9  (74) wing disc ML-DmD16-c3  (52) wing disc ML-DmD21  26 wing disc ML-DmD32  (34) haltere disc ML-DmD17-c3    18 eye-antennal disc ML-DmD11  (68) antennal disc ML-DmD20-c5  27 mixed discs ML-DmD4-c1  (40) CNS ML-DmBG1-c1  (40) CNS ML-DmBG2-c2  (33) tumorous blood cells mbn2    24 ovary fGS/OSS  (45) ovary OSC    23 ovary OSS    22 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    15 Schneider line 2 Sg4    20 embryonic 1182-4H    68 embryonic GM2    52 embryonic Kc167    30 embryonic S1    32 embryonic S3    18 leg disc CME L1    21 wing disc CME-W2    23 wing disc ML-DmD8    61 wing disc ML-DmD9    74 wing disc ML-DmD16-c3    52 wing disc ML-DmD21    26 wing disc ML-DmD32    34 haltere disc ML-DmD17-c3    18 eye-antennal disc ML-DmD11    68 antennal disc ML-DmD20-c5    27 mixed discs ML-DmD4-c1    40 CNS ML-DmBG1-c1    40 CNS ML-DmBG2-c2    33 tumorous blood cells mbn2    24 ovary fGS/OSS    45 ovary OSC    23 ovary OSS    22 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    15 Schneider line 2 Sg4    20 embryonic 1182-4H    68 embryonic GM2    52 embryonic Kc167    30 embryonic S1    32 embryonic S3    18 leg disc CME L1    21 wing disc CME-W2    23 wing disc ML-DmD8    61 wing disc ML-DmD9    74 wing disc ML-DmD16-c3    52 wing disc ML-DmD21    26 wing disc ML-DmD32    34 haltere disc ML-DmD17-c3    18 eye-antennal disc ML-DmD11    68 antennal disc ML-DmD20-c5    27 mixed discs ML-DmD4-c1    40 CNS ML-DmBG1-c1    40 CNS ML-DmBG2-c2    33 tumorous blood cells mbn2    24 ovary fGS/OSS    45 ovary OSC    23 ovary OSS    22 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    14 cold shock, 4-day adult    11 heat shock, 4-day adult    18 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    12 Cadmium 100 mM 48 hrs, 4-day adult    23 Copper 0.5 mM 12 hrs, larvae L3    7 Copper 15 mM 48 hrs, 4-day adult    12 Zinc 5 mM 12 hrs, larvae L3    6 Zinc 4.5 mM 48 hrs, 4-day adult    10 Ethanol 2.5% 3 hrs, larvae L3    30 Ethanol 5% 3 hrs, larvae L3    6 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    6 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    20 Caffeine 25 mg/ml 48 hrs, 4-day adult    11 Paraquat 5 mM 48 hrs, 4-day adult    23 Paraquat 10 mM 48 hrs, 4-day adult    29 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    14 cold shock, 4-day adult    11 heat shock, 4-day adult    18 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    12 Cadmium 100 mM 48 hrs, 4-day adult    23 Copper 0.5 mM 12 hrs, larvae L3    7 Copper 15 mM 48 hrs, 4-day adult    12 Zinc 5 mM 12 hrs, larvae L3    6 Zinc 4.5 mM 48 hrs, 4-day adult    10 Ethanol 2.5% 3 hrs, larvae L3  (30) Ethanol 5% 3 hrs, larvae L3    6 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    6 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    20 Caffeine 25 mg/ml 48 hrs, 4-day adult    11 Paraquat 5 mM 48 hrs, 4-day adult    23 Paraquat 10 mM 48 hrs, 4-day adult  (29) Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    14 cold shock, 4-day adult    11 heat shock, 4-day adult    18 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    12 Cadmium 100 mM 48 hrs, 4-day adult    23 Copper 0.5 mM 12 hrs, larvae L3    7 Copper 15 mM 48 hrs, 4-day adult    12 Zinc 5 mM 12 hrs, larvae L3    6 Zinc 4.5 mM 48 hrs, 4-day adult    10 Ethanol 2.5% 3 hrs, larvae L3    30 Ethanol 5% 3 hrs, larvae L3    6 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    6 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    20 Caffeine 25 mg/ml 48 hrs, 4-day adult    11 Paraquat 5 mM 48 hrs, 4-day adult    23 Paraquat 10 mM 48 hrs, 4-day adult    29 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    14 cold shock, 4-day adult    11 heat shock, 4-day adult    18 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    12 Cadmium 100 mM 48 hrs, 4-day adult    23 Copper 0.5 mM 12 hrs, larvae L3    7 Copper 15 mM 48 hrs, 4-day adult    12 Zinc 5 mM 12 hrs, larvae L3    6 Zinc 4.5 mM 48 hrs, 4-day adult    10 Ethanol 2.5% 3 hrs, larvae L3    30 Ethanol 5% 3 hrs, larvae L3    6 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    6 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    20 Caffeine 25 mg/ml 48 hrs, 4-day adult    11 Paraquat 5 mM 48 hrs, 4-day adult    23 Paraquat 10 mM 48 hrs, 4-day adult    29 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    14 cold shock, 4-day adult    11 heat shock, 4-day adult    18 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    12 Cadmium 100 mM 48 hrs, 4-day adult    23 Copper 0.5 mM 12 hrs, larvae L3    7 Copper 15 mM 48 hrs, 4-day adult    12 Zinc 5 mM 12 hrs, larvae L3    6 Zinc 4.5 mM 48 hrs, 4-day adult    10 Ethanol 2.5% 3 hrs, larvae L3    30 Ethanol 5% 3 hrs, larvae L3    6 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    6 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    20 Caffeine 25 mg/ml 48 hrs, 4-day adult    11 Paraquat 5 mM 48 hrs, 4-day adult    23 Paraquat 10 mM 48 hrs, 4-day adult    29 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    14 cold shock, 4-day adult    11 heat shock, 4-day adult    18 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    12 Cadmium 100 mM 48 hrs, 4-day adult    23 Copper 0.5 mM 12 hrs, larvae L3    7 Copper 15 mM 48 hrs, 4-day adult    12 Zinc 5 mM 12 hrs, larvae L3    6 Zinc 4.5 mM 48 hrs, 4-day adult    10 Ethanol 2.5% 3 hrs, larvae L3  30 Ethanol 5% 3 hrs, larvae L3    6 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    6 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    20 Caffeine 25 mg/ml 48 hrs, 4-day adult    11 Paraquat 5 mM 48 hrs, 4-day adult    23 Paraquat 10 mM 48 hrs, 4-day adult  29 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    14 cold shock, 4-day adult    11 heat shock, 4-day adult    18 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    12 Cadmium 100 mM 48 hrs, 4-day adult    23 Copper 0.5 mM 12 hrs, larvae L3    7 Copper 15 mM 48 hrs, 4-day adult    12 Zinc 5 mM 12 hrs, larvae L3    6 Zinc 4.5 mM 48 hrs, 4-day adult    10 Ethanol 2.5% 3 hrs, larvae L3    30 Ethanol 5% 3 hrs, larvae L3    6 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    6 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    20 Caffeine 25 mg/ml 48 hrs, 4-day adult    11 Paraquat 5 mM 48 hrs, 4-day adult    23 Paraquat 10 mM 48 hrs, 4-day adult    29 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    14 cold shock, 4-day adult    11 heat shock, 4-day adult    18 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    12 Cadmium 100 mM 48 hrs, 4-day adult    23 Copper 0.5 mM 12 hrs, larvae L3    7 Copper 15 mM 48 hrs, 4-day adult    12 Zinc 5 mM 12 hrs, larvae L3    6 Zinc 4.5 mM 48 hrs, 4-day adult    10 Ethanol 2.5% 3 hrs, larvae L3    30 Ethanol 5% 3 hrs, larvae L3    6 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    6 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    20 Caffeine 25 mg/ml 48 hrs, 4-day adult    11 Paraquat 5 mM 48 hrs, 4-day adult    23 Paraquat 10 mM 48 hrs, 4-day adult    29 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_27 mE1_20_mRNA_expression_cluster_02
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0001316 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG17046-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele partially lethal - majority die | pupal stage, with Scer\GAL4pnr-MD237 klarGD9271 viable klar1 klarEY08057 klarEY08325 klarEY13871 klarmarb-BP klarmarb-BX3 klarmarb-HSA.1 Sterility Allele female semi-sterile klarmarb-CD4 fertile klar1 klarEY08057 klarEY08325 klarEY13871 Other Phenotypes Allele flightless klarΔ1-18 locomotor behavior defective | larval stage klarΔ1-18 neuroanatomy defective | pharate adult stage, with Scer\GAL4Ccap.PP klarEY00559 visible klarmarb-BP klarmarb-BX3 visible, with Scer\GAL4GMR.PF klarScer\UAS.P\T.6.T:Hsap\MYC visible, with Scer\GAL4pnr-MD237 klarGD9271 visible | recessive klarmarb-BP klarmarb-BX1 klarmarb-BX2 klarmarb-BX3 klarmarb-BX4 klarmarb-BX5 klarmarb-BX6 klarmarb-BX7 klarmarb-BX8 klarmarb-BX9 klarmarb-BX10 klarmarb-BX11 klarmarb-BX12 klarmarb-BX13 klarmarb-BX14 klarmarb-BX15 klarmarb-BX16 klarmarb-BX17 klarmarb-BX18 klarmarb-BX19 klarmarb-BX20 klarmarb-BX21 klarmarb-CD4 klarmarb-FN1 klarmarb-FQ19 klarmarb-FU2 wild-type klarglrs.6.T:Hsap\MYC Phenotype manifest in Allele A1-7 dorsal acute muscle 1 | embryonic stage 16 klarΔ1-18 A1-7 dorsal acute muscle 2 | embryonic stage 16 klarΔ1-18 A1-7 dorsal oblique muscle 1 | embryonic stage 16 klarΔ1-18 A2-7 dorsal oblique muscle 2 | embryonic stage 16 klarΔ1-18 chaeta | supernumerary, with Scer\GAL4pnr-MD237 klarGD9271 cone cell klarmarb-BX3 klarmarb-CD4 embryonic/larval muscle system | third instar larval stage klarΔ1-18 eye klarglrs.T:Hsap\MYC klarmarb-BP klarmarb-BX1 klarmarb-BX10 klarmarb-BX11 klarmarb-BX12 klarmarb-BX13 klarmarb-BX14 klarmarb-BX15 klarmarb-BX16 klarmarb-BX17 klarmarb-BX18 klarmarb-BX19 klarmarb-BX2 klarmarb-BX20 klarmarb-BX21 klarmarb-BX3 klarmarb-BX4 klarmarb-BX5 klarmarb-BX6 klarmarb-BX7 klarmarb-BX8 klarmarb-BX9 klarmarb-CD4 klarmarb-FN1 klarmarb-FQ19 klarmarb-FU2 eye, with Scer\GAL4GMR.PF klarScer\UAS.P\T.6.T:Hsap\MYC eye (with Df(3L)emc-E12) klarmarb-BP klarmarb-CD4 eye disc klar1 eye disc & nucleus | ectopic klarmarb-BX3 eye photoreceptor cell klarglrs.T:Hsap\MYC klarmarb-CD4 interommatidial bristle klarmarb-CD4 lipid droplet klar1 klarmarb-BP klarmarb-BX3 klarmarb-CD4 lipid particle klar1 klarmarb-BP klarmarb-BX18 klarmarb-BX3 klarmarb-FN1 klarYG3 klarB klarA klarC klarD neurite | pharate adult stage, with Scer\GAL4Ccap.PP klarEY00559 nucleus & cone cell & eye disc klarmarb-CD4 nucleus & eye photoreceptor cell & eye disc klarmarb-CD4 ommatidium klarglrs.T:Hsap\MYC klarmarb-BP photoreceptor cell klarmarb-HSA.1 photoreceptor cell & nucleus klarmarb-CD4 photoreceptor cell & nucleus (with Df(3L)emc-E12) klarmarb-BP klarmarb-CD4 photoreceptor cell R2 klarmarb-CD4 photoreceptor cell R3 klarmarb-CD4 photoreceptor cell R4 klarmarb-CD4 photoreceptor cell R5 klarmarb-CD4 photoreceptor cell R8 klarmarb-CD4 presumptive embryonic salivary gland klarmarb-BP klarmarb-BX3 presumptive embryonic salivary gland, with Scer\GAL4fkh.PH klarScer\UAS.T:Hsap\MYC retina klarmarb-CD4 klarmarb-FN1 klarmarb-FQ19 klarmarb-FU2 rhabdomere klar1 klarmarb-BP klarmarb-CD4 rhabdomere (with Df(3L)emc-E12) klarmarb-BP klarmarb-CD4 rhabdomere & nucleus klarmarb-BX3 scutum & macrochaeta klarmarb-BX19 klarmarb-BX21 klarmarb-BX4 klarmarb-BX5 klarmarb-BX7 klarmarb-BX9 tracheal system klar2679
Classical Alleles ( 65 )
For All Classical Alleles Show
Allele of klar Class Mutagen Stocks Known lesion klar1 loss of function allele 6 Yes klarmarb-CD4 loss of function allele, amorphic allele - genetic evidence ethyl methanesulfonate 2 Yes klarc01127 piggyBac transposase 1 -- klarc01682 piggyBac transposase 1 -- klarc03073 piggyBac transposase 1 -- klarc03763 piggyBac transposase 1 -- klard05910 P-element activity 1 -- klare03231 piggyBac transposase 1 -- klare03389 piggyBac transposase 1 -- klare04076 piggyBac transposase 1 -- klarEY00559 1 -- klarEY08325 P-element activity 1 -- klarEY13871 P-element activity 1 -- klarf02012 piggyBac transposase 1 -- klarf02673 1 -- klarf05922 piggyBac transposase 1 -- klarf06231 piggyBac transposase 1 -- klarf06971 1 -- klarf07257 piggyBac transposase 1 -- klarf07664 piggyBac transposase 1 -- klarKG07384 1 -- klarmarb-BX14 loss of function allele X ray 1 Yes klarmarb-BX3 loss of function allele X ray 1 Yes klarmarb-BX5 loss of function allele X ray 1 Yes klarMB02303 1 -- klarMI00479 1 -- klarMI01364 1 -- klarMI03337 1 -- klarMI06820 1 -- klar2679 ethyl methanesulfonate 0 -- klarA ethyl methanesulfonate 0 Yes klarB ethyl methanesulfonate 0 Yes klarc01711 piggyBac transposase 0 -- klarC ethyl methanesulfonate 0 Yes klarD 0 Yes klarEP3594 P-element activity 0 -- klarEY01576 P-element activity 0 -- klarEY08057 P-element activity 0 -- klarf07272 piggyBac transposase 0 -- klarmarb-BP loss of function allele Delta2-3 0 Yes klarmarb-BX10 loss of function allele X ray 0 -- klarmarb-BX11 loss of function allele X ray 0 -- klarmarb-BX12 loss of function allele X ray 0 Yes klarmarb-BX13 loss of function allele X ray 0 Yes klarmarb-BX15 loss of function allele X ray 0 Yes klarmarb-BX16 loss of function allele X ray 0 -- klarmarb-BX17 loss of function allele X ray 0 -- klarmarb-BX18 X ray 0 Yes klarmarb-BX19 loss of function allele X ray 0 -- klarmarb-BX1 loss of function allele X ray 0 -- klarmarb-BX20 loss of function allele X ray 0 -- klarmarb-BX21 loss of function allele X ray 0 -- klarmarb-BX2 loss of function allele X ray 0 -- klarmarb-BX4 loss of function allele X ray 0 -- klarmarb-BX6 loss of function allele X ray 0 Yes klarmarb-BX7 loss of function allele X ray 0 -- klarmarb-BX8 loss of function allele X ray 0 -- klarmarb-BX9 loss of function allele X ray 0 -- klarmarb-FN1 loss of function allele ethyl methanesulfonate 0 Yes klarmarb-FQ19 loss of function allele ethyl methanesulfonate 0 Yes klarmarb-FU2 loss of function allele ethyl methanesulfonate 0 -- klarmarb-HSA.1 Delta2-3 0 Yes klarMW ethyl methanesulfonate 0 Yes klarYG3 P-element activity 0 Yes klarΔ1-18 FLPase 0 Yes
Alleles Carried on Transgenic Constructs ( 25 )
For All Alleles Carried on Transgenic Constructs Show
Allele of klar Class Mutagen Stocks Known lesion klar3'ΔS.Scer\UAS.6.T:Hsap\MYC in vitro construct - regulatory fusion in vitro construct - coding region fusion 1 Yes klar5'ΔA.Scer\UAS.6.T:Hsap\MYC in vitro construct - regulatory fusion in vitro construct - coding region fusion 1 Yes klarGD9271 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes klarHMS01612 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes klarJF02944 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes klarScer\UAS.P\T.6.T:Hsap\MYC in vitro construct - regulatory fusion in vitro construct - coding region fusion 1 Yes klar15ext.Act5C.T:Disc\RFP-mRFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes klar3'ΔS.glrs.6.T:Hsap\MYC in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes klar5'ΔA.glrs.6.T:Hsap\MYC in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes klarex13-14.Act5C.T:Disc\RFP-mRFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes klarex13-15.15ext.Act5C.T:Disc\RFP-mRFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes klarex14-15.15ext.Act5C.T:Disc\RFP-mRFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes klarex14.ex15.Act5C.T:Disc\RFP-mRFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes klarex15.15ext.Act5C.T:Disc\RFP-mRFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes klarex9-13.Act5C.T:Disc\RFP-mRFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes klarex9-15.15ext.Act5C.T:Disc\RFP-mRFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes klarglrl.T:Hsap\MYC in vitro construct - coding region fusion in vitro construct - regulatory fusion 0 Yes klarglrs.6.T:Hsap\MYC in vitro construct - coding region fusion in vitro construct - regulatory fusion 0 Yes klarglrs.T:Hsap\MYC in vitro construct - coding region fusion in vitro construct - regulatory fusion 0 Yes klarKASH.Act5C.T:Disc\RFP-mRFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes klarScer\UAS.P\T.T:Hsap\MYC in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes klarScer\UAS.P\T.T:Zzzz\TEV.CS.T:Avic\GFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes klarScer\UAS.T:Hsap\MYC in vitro construct - coding region fusion in vitro construct - regulatory fusion 0 Yes klarα.ro in vitro construct - regulatory fusion 0 Yes klarαKASH.Scer\UAS.T:Avic\GFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3L)emc-E12 (Welte et al., 1998, Mosley-Bishop et al., 1999) In(3LR)marbBX1 (Fischer-Vize and Mosley, 1994) In(3LR)marbBX3 (Fischer-Vize and Mosley, 1994) In(3LR)marbBX9 (Fischer-Vize and Mosley, 1994) T(2;3)marbBP (Fischer-Vize and Mosley, 1994) T(2;3)marbBX8 (Fischer-Vize and Mosley, 1994) Tp(3;2)marbBX21 (Fischer-Vize and Mosley, 1994) Tp(?;3)marbBX4 (Fischer-Vize and Mosley, 1994)
Not disrupted in	Df(3L)Ar14-8 (Vize, 1995.6.6)
Partially disrupted in	Df(3L)Δ1-18 (Elhanany-Tamir et al., 2012)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD9271} NA P{TRiP.HMS01612} NA P{TRiP.JF02944} NA P{UAS-klar.3'ΔS} NA P{UAS-klar.5'ΔA} NA P{UAS-klar.M} NA P{UAS-klar.αKASH.GFP} NA P{UASp-klar.6Xmyc} NA P{UASp-klar.GFP} NA P{UASp-klar.MYC} NA characterization construct P{glrl-klar.Myc} NA P{glrs-klar.3'ΔS} NA P{glrs-klar.5'ΔA} NA P{glrs-klar.Myc.6} NA P{glrs-klar.Myc} NA P{ro-klarα} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EP}klarEP3594 NA P{EPgy2}klarEY00559 NA P{EPgy2}klarEY01576 NA P{EPgy2}klarEY08057 NA P{EPgy2}klarEY08325 NA P{EPgy2}klarEY13871 NA P{XP}klard05910 NA miscellaneous insertions Mi{MIC}klarMI00479 NA Mi{MIC}klarMI01364 NA Mi{MIC}klarMI03337 NA Mi{MIC}klarMI06820 NA P{hsp70-boss}klarmarb-HSA.1 NA P{SUPor-P}klarKG07384 NA PBac{PB}klarc01127 NA PBac{PB}klarc01682 NA PBac{PB}klarc01711 NA PBac{PB}klarc03073 NA PBac{PB}klarc03763 NA PBac{RB}klare03231 NA PBac{RB}klare03389 NA PBac{RB}klare04076 NA PBac{WH}klarf02012 NA PBac{WH}klarf02673 NA PBac{WH}klarf05922 NA PBac{WH}klarf06231 NA PBac{WH}klarf06971 NA PBac{WH}klarf07257 NA PBac{WH}klarf07272 NA PBac{WH}klarf07664 NA insertion of enhancer trap binary system Mi{ET1}klarMB02303 No
Gene Ontology: Function, Process & Cellular Component ( 23 unique terms )
Terms Based on Experimental Evidence ( 18 terms )
Molecular Function
CV term Evidence References
protein binding inferred from physical interaction with Lsd-2 (Welte et al., 2005)
Biological Process
CV term Evidence References
cellular membrane organization inferred from mutant phenotype (Myat and Andrew, 2002) compound eye morphogenesis inferred from mutant phenotype (Mosley-Bishop et al., 1999) compound eye photoreceptor development inferred from mutant phenotype (Welte et al., 1998) flight inferred from mutant phenotype (Elhanany-Tamir et al., 2012) larval visceral muscle development inferred from mutant phenotype (Elhanany-Tamir et al., 2012) lipid particle transport along microtubule inferred from mutant phenotype (Guo et al., 2005, Welte et al., 1998) lipid transport inferred from mutant phenotype (Pilot et al., 2006) locomotion inferred from mutant phenotype (Elhanany-Tamir et al., 2012) lumen formation, open tracheal system inferred from mutant phenotype (Myat et al., 2005) nuclear migration inferred from mutant phenotype (Patterson et al., 2004, Guo et al., 2005, Mosley-Bishop et al., 1999, Welte et al., 1998, Kracklauer et al., 2007) organelle localization inferred from mutant phenotype (Elhanany-Tamir et al., 2012) tube morphogenesis inferred from mutant phenotype (Myat and Andrew, 2002)
Cellular Component
CV term Evidence References
cytoplasm inferred from direct assay (Kracklauer et al., 2007) colocalizes_with cytoplasmic microtubule inferred from direct assay (Kracklauer et al., 2007) cytoplasmic microtubule inferred from direct assay (Elhanany-Tamir et al., 2012) colocalizes_with lipid particle inferred from direct assay (Guo et al., 2005) lipid particle inferred from direct assay (Cermelli et al., 2006) nuclear envelope inferred from direct assay (Patterson et al., 2004) colocalizes_with nuclear envelope inferred from direct assay (Guo et al., 2005) perinuclear region of cytoplasm inferred from direct assay (Mosley-Bishop et al., 1999, Kracklauer et al., 2007)
Terms Based on Predictions or Assertions ( 5 terms )
Molecular Function
CV term Evidence References
actin binding inferred from electronic annotation with InterPro:IPR012315 (FlyBase Curators et al., 2004-) ATPase activity, coupled inferred from sequence or structural similarity (Goldstein and Gunawardena, 2000)
Biological Process
CV term Evidence References
microtubule-based movement inferred from sequence or structural similarity (Goldstein and Gunawardena, 2000)
Cellular Component
CV term Evidence References
dynein complex inferred from sequence or structural similarity (Goldstein and Gunawardena, 2000) integral to membrane inferred from electronic annotation with InterPro:IPR012315 (FlyBase Curators et al., 2004-)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions klar allele Gene References klarglrs.T:Hsap\MYC Lam (Patterson et al., 2004) klarScer\UAS.T:Hsap\MYC hkb (Myat and Andrew, 2002)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 63622 DroID - A comprehensive database of gene and protein interactions. • FBgn0001316 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 38067
Orthologs
OrthoDB Orthologs (25) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6M39V2 Dipteran inclusive ortholog search EOG6B8JN6 Insect inclusive ortholog search EOG6W6MR0, EOG66M9FW, EOG6PK14V Arthropod inclusive ortholog search EOG605QG4, EOG63J9MQ Metazoa inclusive ortholog search No orthologs identified
Orthologs in Drosophila Species (EOG6M39V2)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\klar     Drosophila melanogaster fruit fly  Dmel\sob     Drosophila simulans   Dsim\GD25703 Y Y Drosophila simulans   Dsim\GD22744   Y Drosophila sechellia   Dsec\GM14274 Y Y Drosophila erecta   Dere\GG14658 Y Y Drosophila yakuba   Dyak\GE21018 Y Y Drosophila ananassae   Dana\GF24896 Y Y Drosophila pseudoobscura pseudoobscura   Dpse\GA14293 Y Y Drosophila persimilis   Dper\GL22600   Y Drosophila willistoni   Dwil\GK11767   Y Drosophila willistoni   Dwil\GK20119 Y Y Drosophila virilis   Dvir\GJ12478   Y Drosophila mojavensis   Dmoj\GI16733 Y Y Drosophila mojavensis   Dmoj\GI15941   Y Drosophila grimshawi   Dgri\GH15451 Y Y Drosophila grimshawi   Dgri\GH22594   Y
Orthologs in non-Drosophila Dipterans (EOG6B8JN6)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL001296 Y Anopheles gambiae Malaria mosquito  Agam\AGAP009389 Y Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ018680 Y
Orthologs in non-Dipteran Insects (EOG6W6MR0, EOG66M9FW, EOG6PK14V)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Solenopsis invicta Red fire ant  Sinv\SINV11809 Y Solenopsis invicta Red fire ant  Sinv\SINV11824 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI39396 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI42445 Y
Orthologs in non-Insect Arthropods (EOG605QG4, EOG63J9MQ)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Ixodes scapularis Deer tick  Isca\ISCW018627 Y
Orthologs in non-Arthropod Metazoa (None identified)
No non-Arthropod Metazoa orthologies identified
Human Orthologs (0)
Gene OMIM HGNC
AAA Orthologs (9) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD25703 Drosophila sechellia Dsec\GM14274 Drosophila erecta Dere\GG14658 Drosophila yakuba Dyak\GE21018 Drosophila ananassae Dana\GF24896 Drosophila pseudoobscura pseudoobscura Dpse\GA14293 Drosophila willistoni Dwil\GK20119 Drosophila mojavensis Dmoj\GI16733 Drosophila grimshawi Dgri\GH15451
Stocks & Reagents
Stocks Listed in FlyBase ( 40 )
Harvard	- P{XP}klard05910 - PBac{PB}klarc01127 - PBac{PB}klarc01682 - PBac{PB}klarc03073 - PBac{PB}klarc03763 - PBac{RB}klare03231 - PBac{RB}klare03389 - PBac{RB}klare04076 - PBac{WH}klarf02012 - PBac{WH}klarf02673 - PBac{WH}klarf05922 - PBac{WH}klarf06231
	More stocks available...
Genomic Clones ( 2 )
	BACR01I24 BACR18B21 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 99 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GM08528 GM19713 LD36052
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT11467 GH01769 GH07261 GH08428 GH14703 GH22663 GM02132 GM02433 GM03556 GM20077 HL05989 LD13537 RE31577 RE56012 RE66112 RE69871 RH01792 SD10239 SD11423 SD12459 SD15891 SD27948
Other clones	12020 12635 39704 44718 60123 74282 121463 148328 148496 175236 180630 214279.27 214385.28 274336 277157 522756 527243 558026 561823 561931 569116 CK01.19B.D6 CK01.74A.F8 CK01.82B.F9 CK01.82B.H11 CK01.85D.F11 CK01.90B.E11 CK01.105D.F1 CK01.107B.G1 CK01.117A.E2 CK01.123A.E11 Dmel_dig_018923_0601_1168 Dmel_neb_012026_0439_1265 Dmel_neb_013434_0075_1674 Dmel_neb_013589_0608_0946 EC01179 EK018147 EK058256 EK064823 EK064832 EK067922 EK071018 EK093620 EK112814 EK113759 EK156436 EK159848 EK168315 EK241351 EK254234 EK254362 EK279222 EK301727 EK304121 EN02723 EN07509 EN07541 EN12844 EN13214 EN14553 EP02459 EP03252 EP13943 FGH106G02 FGM301G02 GH22034 GM22361 IP04291 IP18140 LD08331 LD24441 LD25931 LD43547 SD02151
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0001316 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 38067
Antibody Information
Other Information
Discoverer
	Nusslein-Volhard.
Etymology
	'klarsicht' is German for 'clear view'. (Jackle and Jahn, 1998)
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: klar marb (Mosley et al., 1998)
Additional comments
Other Comments
	KASH forms of klar function in egg-laying. (Xie and Fischer, 2008) In embryos injected with dsRNA made from templates generated with primers directed against this gene impairs the epithelial clouding phase of lipid droplet transport towards the minus end of microtubules. (Pilot et al., 2006) klar is required for nuclear attachment to the microtubule organizing centre. (Patterson et al., 2004) dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003) snmRNA:438 is derived from the 3' UTR of klar. (Yuan et al., 2003) Area matching Drosophila EST AA696845 (inverted). (Davies, 2001.3.15) klar may associate with Dynein. (Mosley-Bishop et al., 1999) klar may have a direct mechanical role in nuclear migration. (Mosley-Bishop et al., 1999) E. Wieschaus's laboratory has shown that marb is allelic to a previously identified locus named klar. (Mosley et al., 1998) klar facilitates changes in force during lipid droplet transport. (Welte et al., 1998) klar is required for the apical nuclear migrations that accompany cell determination during eye development. The klar mutant phenotype reveals that cell determination does not require nuclear migration. (Fischer-Vize and Mosley, 1994)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_079149 NM_001043111 NM_001103992 NM_001103993 NM_001274282 NM_001274283 NM_001274284 RefSeq (Proteins) • NP_523873 NP_001036576 NP_001097462 NP_001097463 NP_001261211 NP_001261212 NP_001261213 DDBJ / EMBL / GenBank • AA264557 AA696845 AAD43129 AAF47389 AAL28494 ABI31227 ABW08427 ABW08428 AF157066 AGB93906 AGB93907 AGB93908 AW941315 AW941666 AX094211 AY060946 CL528101 CL705938 CL868601 CZ466919 CZ467354 CZ467385 CZ467386 CZ468396 CZ468812 CZ474796 CZ481002 CZ481161 CZ481162 CZ481831 CZ484508 CZ488551 CZ488552 CZ488893 CZ490069 CZ490086 CZ490400 Entrez Gene - A searchable database of RefSeq genes. • 38067 UniProtKB/TrEMBL • A8JNH1 A8JNH2 Q7YU15 Q9W0Q4 Q9Y0E4 Q95S58
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Klarsicht/ANC-1/syne-1 homology (IPR012315)
Linkouts
	BioGRID - A database of protein and genetic interactions • 63622 DroID - A comprehensive database of gene and protein interactions. • FBgn0001316 DRSC - Results from RNAi screens. • FBgn0001316 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0001316 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG17046-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0001316 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 38067 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /cytoskel/klarsicht1.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 38067 modMine - Data generated by the modENCODE project. • FBgn0001316
Synonyms & Secondary IDs ( 9 )
Reported As
Symbol Synonym	CG17046 (Goldstein and Gunawardena, 2000, Guo et al., 2011, Ni et al., 2011.7.19, Perkins et al., 2009) Klar (Guo and Welte, 2002, Guo and Welte, 2001, Welte, 2004, Kim et al., 2006, Cotton et al., 2006, Cotton et al., 2007, Banks and Fischer, 2007, Kracklauer et al., 2007, Guo et al., 2005, Shubeita et al., 2008, Li et al., 2012, Xie and Fischer, 2008, Yu et al., 2011, Li et al., 2012) klar (Jain and Welte, 2007, Guo et al., 2005, Nelson, 2003, Mosley-Bishop et al., 1999, Myat and Andrew, 2002, Abrams et al., 2003, Sudarsan, 2003, Welte et al., 1998, Pilot et al., 2006, Cermelli et al., 2006, Starr and Fischer, 2005, Kracklauer et al., 2007, Meyer et al., 2006, Gan et al., 2010, Fraser et al., 2009, Okamura et al., 2008, Zhao et al., 2008, Dworkin et al., 2009, Technau and Roth, 2008, Johnson and Cagan, 2009, Kracklauer et al., 2010, Hazelett et al., 2009, Elhanany-Tamir et al., 2012) kls (Michaut et al., 2003, Gonzalez et al., 2002, Lindsley and Zimm, 1992) marb (Fischer, 1993, Fischer-Vize and Mosley, 1994, Fischer and Rubin, 1990, )
Name Synonym	Klarischt (Elhanany-Tamir et al., 2012) klarsicht (Czech et al., 2008, Jain and Welte, 2007, Mosley-Bishop et al., 1999, Morris, 2000, Starr and Fischer, 2005, Fraser et al., 2009, Zhou et al., 2009) Klarsicht (Banks et al., 2006, Cotton et al., 2007, Banks and Fischer, 2007, Morris, 2003, Kracklauer et al., 2007, Kracklauer et al., 2007, Starr and Han, 2003, Xie and Fischer, 2008, Technau and Roth, 2008, Harbison et al., 2005, Yu et al., 2011) marbles (Thomas and Wassarman, 1999, Fischer, 1993, Fischer-Vize and Mosley, 1994, Fischer and Rubin, 1990, Mosley-Bishop et al., 1999, Starr and Fischer, 2005)
Secondary FlyBase IDs
	FBgn0002650
References ( 111 )
Generate a list of	All references relating to this gene ( 111 )
List References by type	Research paper  ( 41 ) Supplementary material  ( 8 ) Review  ( 14 ) Personal communication to FlyBase  ( 15 ) Abstract  ( 25 ) FlyBase analysis  ( 3 ) Book  ( 1 ) Patent  ( 1 ) DNA/RNA sequence record  ( 2 ) Interview  ( 1 )
Recent research papers ( 5 )
	Busser et al., 2012, PLoS Genet. 8(3): e1002531 A machine learning approach for identifying novel cell type-specific transcriptional regulators of myogenesis. [FBrf0217716] Elhanany-Tamir et al., 2012, J. Cell Biol. 198(5): 833--846 Organelle positioning in muscles requires cooperation between two KASH proteins and microtubules. [FBrf0219334] Li et al., 2012, Curr. Biol. 22(22): 2104--2113 Lipid droplets control the maternal histone supply of Drosophila embryos. [FBrf0220040] Guo et al., 2011, Mol. Cell. Biol. 31(4): 639--651 Poly(A) Signals Located near the 5' End of Genes Are Silenced by a General Mechanism That Prevents Premature 3'-End Processing. [FBrf0212905] Yu et al., 2011, BMC Cell Biol. 12: 9 Targeting the motor regulator Klar to lipid droplets. [FBrf0213230] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...