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Gene Dmel\kn

General Information
Symbol	Dmel\kn	Species	D. melanogaster
Name	knot	Annotation symbol	CG10197
Feature type	protein_coding_gene	FlyBase ID	FBgn0001319
Gene Model Status	Current	Stock availability	11 publicly available
Also Known As	Col
Genomic Location
Chromosome (arm)	2R	Recombination map	2-72.3
Cytogenetic map	51C2-51C3	Sequence location	2R:10,660,152..10,694,566 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene knot is referred to in FlyBase by the symbol Dmel\kn (CG10197, FBgn0001319). It is a protein_coding_gene from Drosophila melanogaster. It is reported to have molecular function: sequence-specific DNA binding transcription factor activity. There is experimental evidence that it is involved in the biological process: innate immune response; response to symbiont; regulation of transcription, DNA-dependent; specification of segmental identity, intercalary segment; head segmentation; dendrite morphogenesis; regulation of lamellocyte differentiation; embryonic development via the syncytial blastoderm; imaginal disc-derived wing vein specification. 25 alleles are reported. The phenotypes of these alleles are annotated with: adult segment; hypodermal muscle of larval abdomen; organ system subdivision; organ system; adult mesothoracic segment; A1-7 oblique muscle; cephalopharyngeal skeleton; peripheral nervous system; larval abdominal segment 5; nervous system. It has 4 annotated transcripts and 4 annotated polypeptides. Protein features are: Cell surface receptor IPT/TIG; Helix-loop-helix DNA-binding; Immunoglobulin E-set; Immunoglobulin-like fold; Transcription factor COE; Transcription factor COE, conserved site. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderate expression to a trough of extremely low expression. Peak expression observed at stages throughout embryogenesis. Summary of FlyAtlas Anatomical Expression Data: Two or more Affy2 ProbeSets identify exons of this gene. This is a summary of the tissue expression peaks exhibited in at least one of these ProbeSets. Expression at moderate levels in the following post-embryonic organs or tissues: larval/adult central nervous system, adult heart. Gene sequence location is 2R:10660152..10694566.
External Summaries
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	kn: knot Veins L3 and L4 shifted closer together in region of anterior crossvein, which is either extremely thick or eliminated by regional fusion of L3 and L4. Frequently, extra crossvein between L3 and L4 near end of wing. Shift in positions of sensilla and extra chaetae accompanies shift in vein positions. Wing narrowed. Head narrowed and flattened, so the long axis of eye is at oblique angle. May overlap wild type at high temperatures and in late counts. Best at 19. RK2.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2011_10	References Du et al., 2011, PLoS ONE 6(9): e24168 Wojcinski et al., 2011, Dev. Biol. 358(1): 168--180
FB2012_01	References Demilly et al., 2011, PLoS ONE 6(6): e21213 Benito-Sipos et al., 2011, Development 138(24): 5311--5320 Sequence features HFA07637 BKN41716 BKN29184 HFA05507 HFA06196 BKN34322
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 51C2-51C3   Limits computationally determined from genome sequence between P{lacW}Rpn6k00103 and P{PZ}chn02064
Experimentally Determined Cytological Location
Cytogenetic map Notes References 51C1-51C2   (determined by in situ hybridisation)   (Crozatier et al., 1999) 51E-51F   (determined by in situ hybridisation)   (Kania et al., 1995) 51C1-51C2   (determined by in situ hybridisation)   (Vincent, 1995) 51B10-51C1   Placed in 51B10--51C1 by deficiency mapping (details unspecified).   (Crozatier et al., 1996)
Experimentally Determined Recombination Data
Location	2-72.3
Left of (cM)
Right of (cM)	L (Nestoras et al., 1997)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\kn for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) kn-RA FBtr0087465  NM_080074   3458   557 kn-RB FBtr0112809  NM_166070   3927   575 kn-RC FBtr0112810  NM_001103847   3339   689 kn-RD FBtr0301400  NM_137146   3455   556
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein kn-PA   FBpp0086595   60.5   557   8.69     NP_524813   AAF58204 kn-PB   FBpp0111721   62.5   575   8.53     NP_725419   AAM68551 kn-PC   FBpp0111722   74.8   689   8.41     NP_001097317   ABV53800 kn-PD   FBpp0290614   60.4   556   8.69     NP_610990   AAF58203
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Transcription factor COE, conserved site (IPR018350) Cell surface receptor IPT/TIG (IPR002909) Transcription factor COE (IPR003523) Immunoglobulin E-set (IPR014756) Helix-loop-helix DNA-binding (IPR011598) Immunoglobulin-like fold (IPR013783)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE013599 AAF58203   AE013599 AAF58204   AE013599 AAM68551   AE013599 ABV53800   AI258364   AY119102 AAM50962   BI164134   BI234222   BI240518   BI577611   BI577655   BI592030   CL526130   CO179589   CO179722   CO266449   CO285735   CO288277   CO289739   CO311535   CO333844   EC058463   EC067982   EC069932   EC087092   EC196710   EC207762   EC213599   EC218811   EC218974   EC244867   GH951451   GH961873   GH963624   GH963776   GH964755   GH965185   GH967794   GH970818   GH971176   GH971218   GH979459   GH979625   GH984554   X97803
UniProtKB/Swiss-Prot	P56721
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage   head mesoderm   (Seecoomar et al., 2000) embryonic mandibular segment   (Seecoomar et al., 2000) embryonic stage 4 -- 7   intercalary segment | precursor   (Crozatier et al., 1999) Comment:reference states "parasegment 0" parasegment 1 | anterior to   (Crozatier et al., 1999) embryonic stage 8 -- 11   embryonic mandibular segment   (Seecoomar et al., 2000) mitotic domain 2 of embryonic mandibular segment   (Seecoomar et al., 2000) embryonic mandibular segment | anterior   (Seecoomar et al., 2000) embryonic stage 10 -- 11   hypopharyngeal lobe | restricted   (Crozatier et al., 1999) third instar larval stage   wing disc | anterior/posterior compartment boundary   (Glise et al., 2002)
Additional Descriptive Data
	kn transcripts are expressed in the anterior-most regions of the mandibular segment and also in the cephalic mesoderm. (Seecoomar et al., 2000) Transcript is detected in 7 cells anterior to the A/P boundary in third instar wing discs. However, expression is excluded from the D/V boundary that will form the wing margin. (Glise et al., 2002)
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
immunolocalization Stage Tissue/Position (including subcellular localization) Reference embryonic stage 12 -- 16   eo neuron   (Orgogozo et al., 2004) embryonic stage 15 -- larval stage   Tvb neuron   (Baumgardt et al., 2007) Tv neuron   (Baumgardt et al., 2007) third instar larval stage   wing disc | anterior/posterior compartment boundary   (Glise et al., 2002)
Additional Descriptive Data
	Protein is detected in 7 cells anterior to the A/P boundary in third instar wing discs. However, expression is excluded from the D/V boundary that will form the wing margin. (Glise et al., 2002)
Marker for
	eo neuron
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0001319    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0001319 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderate expression to a trough of extremely low expression. Peak expression observed at stages throughout embryogenesis. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0001319 expression level Developmental Stage   Expression Level embryo 00-02hr    19 embryo 02-04hr    115 embryo 04-06hr    483 embryo 06-08hr    624 embryo 08-10hr    686 embryo 10-12hr    1092 embryo 12-14hr    1014 embryo 14-16hr    1043 embryo 16-18hr    880 embryo 18-20hr    843 embryo 20-22hr    547 embryo 22-24hr    556 larva L1    393 larva L2    108 larva L3 12hr old    56 larva L3 puffstage 1-2    38 larva L3 puffstage 3-6    91 larva L3 puffstage 7-9    125 white prepupae new    156 white prepupae 12hr    161 white prepupae 24hr    206 pupae 2d postWPP    180 pupae 3d postWPP    67 pupae 4d postWPP    50 adult male 01day    42 adult male 05day    54 adult male 30day    39 adult female 01day    25 adult female 05day    7 adult female 30day    13 Expression Level Scale  None   Extremely low   Very low   Low   Moderate  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr    19 embryo 02-04hr  (115) embryo 04-06hr  (483) embryo 06-08hr  (624) embryo 08-10hr  (686) embryo 10-12hr  (1092) embryo 12-14hr  (1014) embryo 14-16hr  (1043) embryo 16-18hr  (880) embryo 18-20hr  (843) embryo 20-22hr  (547) embryo 22-24hr  (556) larva L1  (393) larva L2  (108) larva L3 12hr old    56 larva L3 puffstage 1-2    38 larva L3 puffstage 3-6    91 larva L3 puffstage 7-9  (125) white prepupae new  (156) white prepupae 12hr  (161) white prepupae 24hr  (206) pupae 2d postWPP  (180) pupae 3d postWPP    67 pupae 4d postWPP    50 adult male 01day    42 adult male 05day    54 adult male 30day    39 adult female 01day    25 adult female 05day    7 adult female 30day    13 Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    19 embryo 02-04hr    115 embryo 04-06hr    483 embryo 06-08hr    624 embryo 08-10hr    686 embryo 10-12hr    1092 embryo 12-14hr    1014 embryo 14-16hr    1043 embryo 16-18hr    880 embryo 18-20hr    843 embryo 20-22hr    547 embryo 22-24hr    556 larva L1    393 larva L2    108 larva L3 12hr old    56 larva L3 puffstage 1-2    38 larva L3 puffstage 3-6    91 larva L3 puffstage 7-9    125 white prepupae new    156 white prepupae 12hr    161 white prepupae 24hr    206 pupae 2d postWPP    180 pupae 3d postWPP    67 pupae 4d postWPP    50 adult male 01day    42 adult male 05day    54 adult male 30day    39 adult female 01day    25 adult female 05day    7 adult female 30day    13 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    19 embryo 02-04hr    115 embryo 04-06hr    483 embryo 06-08hr    624 embryo 08-10hr    686 embryo 10-12hr    1092 embryo 12-14hr    1014 embryo 14-16hr    1043 embryo 16-18hr    880 embryo 18-20hr    843 embryo 20-22hr    547 embryo 22-24hr    556 larva L1    393 larva L2    108 larva L3 12hr old    56 larva L3 puffstage 1-2    38 larva L3 puffstage 3-6    91 larva L3 puffstage 7-9    125 white prepupae new    156 white prepupae 12hr    161 white prepupae 24hr    206 pupae 2d postWPP    180 pupae 3d postWPP    67 pupae 4d postWPP    50 adult male 01day    42 adult male 05day    54 adult male 30day    39 adult female 01day    25 adult female 05day    7 adult female 30day    13 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    19 embryo 02-04hr    115 embryo 04-06hr    483 embryo 06-08hr    624 embryo 08-10hr    686 embryo 10-12hr    1092 embryo 12-14hr    1014 embryo 14-16hr    1043 embryo 16-18hr    880 embryo 18-20hr    843 embryo 20-22hr    547 embryo 22-24hr    556 larva L1    393 larva L2    108 larva L3 12hr old    56 larva L3 puffstage 1-2    38 larva L3 puffstage 3-6    91 larva L3 puffstage 7-9    125 white prepupae new    156 white prepupae 12hr    161 white prepupae 24hr    206 pupae 2d postWPP    180 pupae 3d postWPP    67 pupae 4d postWPP    50 adult male 01day    42 adult male 05day    54 adult male 30day    39 adult female 01day    25 adult female 05day    7 adult female 30day    13 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0001319 expression level Developmental Stage   Expression Level embryo 00-02hr    19 embryo 02-04hr    115 embryo 04-06hr    483 embryo 06-08hr    624 embryo 08-10hr    686 embryo 10-12hr    1092 embryo 12-14hr    1014 embryo 14-16hr    1043 embryo 16-18hr    880 embryo 18-20hr    843 embryo 20-22hr    547 embryo 22-24hr    556 larva L1    393 larva L2    108 larva L3 12hr old    56 larva L3 puffstage 1-2    38 larva L3 puffstage 3-6    91 larva L3 puffstage 7-9    125 white prepupae new    156 white prepupae 12hr    161 white prepupae 24hr    206 pupae 2d postWPP    180 pupae 3d postWPP    67 pupae 4d postWPP    50 adult male 01day    42 adult male 05day    54 adult male 30day    39 adult female 01day    25 adult female 05day    7 adult female 30day    13 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr    19 embryo 02-04hr  115 embryo 04-06hr  (483) embryo 06-08hr  (624) embryo 08-10hr  (686) embryo 10-12hr  (1092) embryo 12-14hr  (1014) embryo 14-16hr  (1043) embryo 16-18hr  (880) embryo 18-20hr  (843) embryo 20-22hr  (547) embryo 22-24hr  (556) larva L1  (393) larva L2  108 larva L3 12hr old    56 larva L3 puffstage 1-2    38 larva L3 puffstage 3-6    91 larva L3 puffstage 7-9  125 white prepupae new  (156) white prepupae 12hr  (161) white prepupae 24hr  (206) pupae 2d postWPP  (180) pupae 3d postWPP    67 pupae 4d postWPP    50 adult male 01day    42 adult male 05day    54 adult male 30day    39 adult female 01day    25 adult female 05day    7 adult female 30day    13 Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    19 embryo 02-04hr    115 embryo 04-06hr    483 embryo 06-08hr    624 embryo 08-10hr    686 embryo 10-12hr    1092 embryo 12-14hr    1014 embryo 14-16hr    1043 embryo 16-18hr    880 embryo 18-20hr    843 embryo 20-22hr    547 embryo 22-24hr    556 larva L1    393 larva L2    108 larva L3 12hr old    56 larva L3 puffstage 1-2    38 larva L3 puffstage 3-6    91 larva L3 puffstage 7-9    125 white prepupae new    156 white prepupae 12hr    161 white prepupae 24hr    206 pupae 2d postWPP    180 pupae 3d postWPP    67 pupae 4d postWPP    50 adult male 01day    42 adult male 05day    54 adult male 30day    39 adult female 01day    25 adult female 05day    7 adult female 30day    13 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    19 embryo 02-04hr    115 embryo 04-06hr    483 embryo 06-08hr    624 embryo 08-10hr    686 embryo 10-12hr    1092 embryo 12-14hr    1014 embryo 14-16hr    1043 embryo 16-18hr    880 embryo 18-20hr    843 embryo 20-22hr    547 embryo 22-24hr    556 larva L1    393 larva L2    108 larva L3 12hr old    56 larva L3 puffstage 1-2    38 larva L3 puffstage 3-6    91 larva L3 puffstage 7-9    125 white prepupae new    156 white prepupae 12hr    161 white prepupae 24hr    206 pupae 2d postWPP    180 pupae 3d postWPP    67 pupae 4d postWPP    50 adult male 01day    42 adult male 05day    54 adult male 30day    39 adult female 01day    25 adult female 05day    7 adult female 30day    13 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    19 embryo 02-04hr    115 embryo 04-06hr    483 embryo 06-08hr    624 embryo 08-10hr    686 embryo 10-12hr    1092 embryo 12-14hr    1014 embryo 14-16hr    1043 embryo 16-18hr    880 embryo 18-20hr    843 embryo 20-22hr    547 embryo 22-24hr    556 larva L1    393 larva L2    108 larva L3 12hr old    56 larva L3 puffstage 1-2    38 larva L3 puffstage 3-6    91 larva L3 puffstage 7-9    125 white prepupae new    156 white prepupae 12hr    161 white prepupae 24hr    206 pupae 2d postWPP    180 pupae 3d postWPP    67 pupae 4d postWPP    50 adult male 01day    42 adult male 05day    54 adult male 30day    39 adult female 01day    25 adult female 05day    7 adult female 30day    13 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0001319    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0001319 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Two or more Affy2 ProbeSets identify exons of this gene. This is a summary of the tissue expression peaks exhibited in at least one of these ProbeSets. Expression at moderate levels in the following post-embryonic organs or tissues: larval/adult central nervous system, adult heart. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Heatmap Tissue   Expression Level Larval Central Nervous System no informative data Larval Midgut     Larval Hindgut     Larval Malpighian Tubules no informative data Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass no informative data Adult Head no informative data Adult Eye     Adult Brain no informative data Adult Thoracic-Abdominal Ganglion no informative data Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body no informative data Adult Salivary Gland     Adult Heart no informative data Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass no informative data modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_11
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0001319 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • HDC06452;CG10197-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | embryonic stage knSA1 lethal | embryonic stage | heat sensitive knhs.PS lethal | embryonic stage | heat sensitive, with Scer\GAL4srp.D.cCa knScer\UAS.cVa lethal | embryonic stage | recessive knKN1 knKN2 knKN3 knKN4 knunspecified lethal | late embryonic stage (with kn1) knKN2 lethal | late embryonic stage (with knKN2) kn1 lethal | recessive kncol-1 kncol-2 kncol-3 viable knSA2 viable, with Scer\GAL4pnr-MD237 knGD4730 Other Phenotypes Allele immune response defective, with kn+m5 kncol-1 immune response defective, with kncol-1 kn+m5 neuroanatomy defective, with Scer\GAL4221 knScer\UAS.cMa neuroanatomy defective, with Scer\GAL4smid-C161 knScer\UAS.cMa neuroanatomy defective | somatic clone kn1 knKN2 neuroanatomy defective | somatic clone, with Scer\GAL421-7 knScer\UAS.cMa neuroanatomy defective | somatic clone (with kn1) knKN2 neuroanatomy defective | somatic clone (with knKN2) kn1 smell perception defective kncol-85 visible kn1 kn+m5 knunspecified visible, with kn+m5 kncol-1 visible, with kncol-1 kn+m5 visible, with Scer\GAL4Bx-MS1096 knScer\UAS.cMa visible, with Scer\GAL4dpp.blk1 knScer\UAS.cMa visible (with kn1) kncol-1 visible (with kncol-1) kn1 visible | cell non-autonomous | recessive | somatic clone knKN4 visible | recessive kn1 knSA1 visible | somatic clone kncol-1 knKN1 knKN2 knKN3 knKN4 knunspecified wild-type kn+m5 Phenotype manifest in Allele 1st posterior cell kn+m5 kn1 knSA1 kncol-1 1st posterior cell, with kn+m5 kncol-1 1st posterior cell, with kncol-1 kn+m5 1st posterior cell (with kn1) kncol-1 1st posterior cell (with kncol-1) kn1 2nd posterior cell, with kn+m5 kncol-1 2nd posterior cell, with kncol-1 kn+m5 2nd posterior cell (with kn1) kncol-1 2nd posterior cell (with kncol-1) kn1 3rd posterior cell, with kn+m5 kncol-1 3rd posterior cell, with kncol-1 kn+m5 3rd posterior cell (with kn1) kncol-1 3rd posterior cell (with kncol-1) kn1 abdominal 1 dorsal acute muscle 3 kncol-1 kncol-2 kncol-3 knhs.PS abdominal 1 dorsal oblique muscle 5 knhs.PS abdominal 2 dorsal acute muscle 3 kncol-1 kncol-2 kncol-3 knhs.PS abdominal 2 dorsal oblique muscle 5 knhs.PS abdominal 3 dorsal acute muscle 3 kncol-1 kncol-2 kncol-3 knhs.PS abdominal 3 dorsal oblique muscle 5 knhs.PS abdominal 4 dorsal acute muscle 3 kncol-1 kncol-2 kncol-3 knhs.PS abdominal 4 dorsal oblique muscle 5 knhs.PS abdominal 5 dorsal acute muscle 3 kncol-1 kncol-2 kncol-3 knhs.PS abdominal 5 dorsal oblique muscle 5 knhs.PS abdominal 6 dorsal acute muscle 3 kncol-1 kncol-2 kncol-3 knhs.PS abdominal 6 dorsal oblique muscle 5 knhs.PS abdominal 7 dorsal acute muscle 3 kncol-1 kncol-2 kncol-3 knhs.PS abdominal 7 dorsal oblique muscle 5 knhs.PS anterior crossvein kn+m5 kn1 kncol-1 cephalopharyngeal skeleton knKN2 knKN4 kncol-1 kncol-2 kncol-3 cephalopharyngeal skeleton | heat sensitive knhs.PS class I dendritic arborizing neuron, with Scer\GAL4221 knScer\UAS.cMa class II dendritic arborizing neuron, with Scer\GAL4smid-C161 knScer\UAS.cMa class III dendritic arborizing neuron, with Scer\GAL4smid-C161 knScer\UAS.cMa class IV dendritic arborizing neuron | somatic clone kn1 class IV dendritic arborizing neuron | somatic clone (with kn1) knKN2 class IV dendritic arborizing neuron | somatic clone (with knKN2) kn1 crystal cell, with Scer\GAL4srp.D.cCa knScer\UAS.cVa dendrite, with Scer\GAL4221 knScer\UAS.cMa dendrite, with Scer\GAL4smid-C161 knScer\UAS.cMa dendrite | somatic clone kn1 knKN2 dendrite | somatic clone (with kn1) knKN2 dendrite | somatic clone (with knKN2) kn1 dendritic arborizing neuron | somatic clone, with Scer\GAL421-7 knScer\UAS.cMa dorsal multidendritic neuron ddaC | somatic clone kn1 knKN2 dorsal multidendritic neuron ddaC | somatic clone (with kn1) knKN2 dorsal multidendritic neuron ddaC | somatic clone (with knKN2) kn1 embryonic/larval lymph gland kncol-1 embryonic/larval lymph gland, with Scer\GAL4dome-PG125 knScer\UAS.cVa embryonic/larval somatic muscle kncol-1 kncol-2 kncol-3 embryonic head kncol-1 kncol-2 kncol-3 hypopharyngeal lobe knKN4 hypostomal sclerite | heat sensitive knhs.PS lamellocyte | supernumerary, with Scer\GAL4srp.D.cCa knScer\UAS.cVa lateral process knKN2 knKN4 kna.hs kncol-1 kncol-2 kncol-3 lateral process | heat sensitive knhs.PS macrochaeta | ectopic | somatic clone knKN1 knKN2 knKN3 knKN4 melanotic mass | ectopic, with Scer\GAL4srp.D.cCa knScer\UAS.cVa mesothoracic dorsal acute muscle 4 kncol-1 kncol-2 kncol-3 metathoracic dorsal acute muscle 4 kncol-1 kncol-2 kncol-3 posterior crossvein kn+m5 kncol-1 sensillum campaniformium of dorsal radius | ectopic knKN1 knKN2 knKN3 knKN4 submarginal cell, with kn+m5 kncol-1 submarginal cell, with kncol-1 kn+m5 ventral arm of pharyngeal sclerite knKN2 knKN4 wing knSA2 knunspecified wing, with kn+m5 kncol-1 wing, with kncol-1 kn+m5 wing | anterior compartment | somatic clone knKN1 knKN2 knKN3 knKN4 wing | cell non-autonomous | somatic clone knKN4 wing | P-stage, with kn+m5 kncol-1 wing | P-stage, with kncol-1 kn+m5 wing | P-stage (with kn1) kncol-1 wing | P-stage (with kncol-1) kn1 wing basal cell 1 kn1 knSA1 wing cell knSA2 wing cell & wing vein L3 & wing vein L4 kn1 wing cell & wing vein L3 & wing vein L4 | somatic clone knKN1 knKN2 knKN3 knKN4 wing margin bristle | somatic clone knKN1 knKN2 knKN3 knKN4 wing vein kn+m5 kncol-1 knunspecified wing vein | cell non-autonomous | ectopic | somatic clone knKN4 wing vein | ectopic knKN1 knKN2 knKN3 knKN4 wing vein | ectopic | somatic clone knKN1 knKN2 knKN3 knKN4 knunspecified wing vein L2, with Scer\GAL4Bx-MS1096 knScer\UAS.cMa wing vein L3 kn+m5 kn1 knSA1 kncol-1 wing vein L3, with kn+m5 kncol-1 wing vein L3, with kncol-1 kn+m5 wing vein L3, with Scer\GAL4ap-md544 kndsRNA.Scer\UAS wing vein L3, with Scer\GAL4Bx-MS1096 knScer\UAS.cMa wing vein L3 | distal, with Scer\GAL4dpp.blk1 knScer\UAS.cMa wing vein L3 | somatic clone knunspecified wing vein L4 kn+m5 kn1 knSA1 kncol-1 wing vein L4, with kn+m5 kncol-1 wing vein L4, with kncol-1 kn+m5 wing vein L4, with Scer\GAL4ap-md544 kndsRNA.Scer\UAS wing vein L4 | cell non-autonomous | somatic clone knKN1 knKN2 knKN3 knKN4 wing vein L4 | distal, with Scer\GAL4Bx-MS1096 knScer\UAS.cMa wing vein L4 | proximal, with Scer\GAL4Bx-MS1096 knScer\UAS.cMa wing vein L4 | somatic clone kncol-1 knunspecified wing vein L5, with Scer\GAL4en-e16E knScer\UAS.cVa wing vein L5 | distal, with Scer\GAL4Bx-MS1096 knScer\UAS.cMa wing vein L5 | proximal, with Scer\GAL4Bx-MS1096 knScer\UAS.cMa
Classical Alleles ( 14 )
For All Classical Alleles Show
Allele of kn Class Mutagen Stocks Known lesion kncol-1 amorphic allele - genetic evidence, loss of function allele ethyl methanesulfonate 2 Yes kn1 2 -- knEY09641 P-element activity 1 -- knKN4 ethyl methanesulfonate 1 -- knMI02387 1 -- knSA2 gamma ray 1 Yes kncol-2 loss of function allele ethyl methanesulfonate 0 -- kncol-3 loss of function allele ethyl methanesulfonate 0 -- kncol-85 P-element activity 0 -- knKN1 ethyl methanesulfonate 0 -- knKN2 ethyl methanesulfonate 0 -- knKN3 ethyl methanesulfonate 0 -- knSA1 X ray 0 -- knunspecified 0 --
Alleles Carried on Transgenic Constructs ( 11 )
For All Alleles Carried on Transgenic Constructs Show
Allele of kn Class Mutagen Stocks Known lesion knJF02206 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes knKK114322 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes knScer\UAS.cMa in vitro construct - regulatory fusion 1 Yes kn+m5 in vitro construct - regulatory fusion 0 Yes kna.hs in vitro construct - regulatory fusion 0 Yes kndsRNA.Scer\UAS in vitro construct - regulatory fusion in vitro construct - RNAi 0 Yes knGD4730 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes knhs.PS in vitro construct - regulatory fusion 0 Yes knKK109184 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes knScer\UAS.cVa in vitro construct - regulatory fusion 0 Yes knt5 in vitro construct - genomic fragment 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2R)BSC429 (Christensen et al., 2008.4.15) Df(2R)BSC858 (Christensen et al., 2009.7.22) Df(2R)knSA3 (Mohler, 1997.8.7) In(2R)knSA1 (Nestoras et al., 1997) T(1;2)knSA2 (Seecoomar et al., 2000)
Not disrupted in	Df(2R)Jp1 (Nestoras et al., 1997) Df(2R)trix (Nestoras et al., 1997)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data reporter construct P{col5-lacZ.C} No P{kn-lacZ.1-2330} No P{kn-lacZ.-20} No P{kn-lacZ.224-1426} No P{kn-lacZ.701-1835} No P{kn-lacZ.701-1991.Ci1047} No P{kn-lacZ.701-1991.Ci1341} No P{kn-lacZ.701-1991.Ci1680} No P{kn-lacZ.701-1991.KO} No P{kn-lacZ.701-1991.KOmut} No P{kn-lacZ.701-1991.mut} No P{kn-lacZ.701-1991.UBX1KO} No P{kn-lacZ.701-1991.UBX2KO} No P{kn-lacZ.701-1991.UBX3KO} No P{kn-lacZ.701-1991.UBX4KO} No P{kn-lacZ.701-1991} No P{kn-lacZ.1599-1991} No P{kn-lacZ.composite} No P{kn-lacZ.compositeKO} No heat-shock construct P{hs-col.S} NA P{HscolA} NA UAS construct P{GD4730} NA P{GD7846} NA P{GD17582} NA P{KK109184} NA P{KK114322} NA P{TRiP.JF02206} NA P{UAS-col.dsRNA} NA P{UAS-kn.M} NA P{UAS-kn.V} NA characterization construct P{C4-gBF} NA P{col5-cDNA.C} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EPgy2}knEY09641 NA insertion of enhancer trap P{lArB}kncol-85 No miscellaneous insertions Mi{MIC}knMI02387 NA
Gene Ontology: Function, Process & Cellular Component ( 17 unique terms )
Terms Based on Experimental Evidence ( 9 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
dendrite morphogenesis inferred from genetic interaction with ct (Jinushi-Nakao et al., 2007) inferred from mutant phenotype (Jinushi-Nakao et al., 2007) embryonic development via the syncytial blastoderm inferred from mutant phenotype (Crozatier et al., 1999) head segmentation inferred from mutant phenotype (Crozatier et al., 1999) imaginal disc-derived wing vein specification inferred from mutant phenotype (Crozatier et al., 2002) innate immune response inferred from mutant phenotype (Crozatier et al., 2004) regulation of lamellocyte differentiation inferred from mutant phenotype (Crozatier et al., 2004) regulation of transcription, DNA-dependent inferred from mutant phenotype (Crozatier et al., 1999) response to symbiont inferred from mutant phenotype (Crozatier et al., 2004) specification of segmental identity, intercalary segment inferred from mutant phenotype (Crozatier et al., 1999)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 9 terms )
Molecular Function
CV term Evidence References
DNA binding inferred from electronic annotation with InterPro:IPR003523 (FlyBase Curators et al., 2004-) sequence-specific DNA binding transcription factor activity non-traceable author statement (Swiss-Prot Project Members, 2001.8.20)
Biological Process
CV term Evidence References
anterior head segmentation traceable author statement (Peel, 2004) blastoderm segmentation traceable author statement (Urbach and Technau, 2004) imaginal disc-derived wing morphogenesis traceable author statement (de Celis, 2003) pattern specification process traceable author statement (Potter and Xu, 2001) posterior head segmentation traceable author statement (Peel, 2004) regulation of transcription, DNA-dependent traceable author statement (de Celis, 2003)
Cellular Component
CV term Evidence References
nucleus non-traceable author statement (Swiss-Prot Project Members, 2001.8.20)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions kn allele Gene References kn1 Egfr (Diaz-Benjumea and Garcia-Bellido, 1990) hh (Vervoort et al., 1999) ptc (Nestoras et al., 1997) kncol-1 fu (Vervoort et al., 1999) hh (Vervoort et al., 1999) knKN4 ptc (Nestoras et al., 1997) knScer\UAS.cVa ci (Vervoort et al., 1999) unspecified rho (Sturtevant and Bier, 1995)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 69588 DroID - A comprehensive database of gene and protein interactions. • FBgn0001319 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 45318
Orthologs
Genome-wide drosophilid orthologs	Dana\GF11778 Dere\GG22397 Dgri\GH20479 Dmoj\GI21022 Dper\GL10656 Dpse\GA24435 Dsec\GM20182 Dsim\GD25656 Dvir\GJ21945 Dwil\GK23295 Dyak\GE12286
Curated drosophilid orthologs	Dpse\GA10148
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AAEL003436-PA Aedes aegypti (mosquito) AGAP011437-PA Anopheles gambiae (mosquito) XP_394171.3 Apis mellifera (honey bee) XP_001946640.1 Acyrthosiphon pisum (pea aphid) ENSBTAP00000042518 Bos taurus (bovine) CN16160 Caenorhabditis brenneri (small nematode) CBP33933 Caenorhabditis briggsae (nematode) CE29366 Caenorhabditis elegans (worm) ENSCAFP00000009901 Canis familiaris (dog) ENSCINP00000006573 Ciona intestinalis (sea squirt) JA13194 Caenorhabditis japonica (nematode) CPIJ010142 Culex pipens (mosquito) ENSCPOP00000018411 Cavia porcellus (guinea pig) RP27403 Caenorhabditis remanei (nematode) ENSCSAVP00000000810 Ciona savignyi (sedentary tunicate) 93575 Capitella spl (polychaete worm) 22285 Daphnia pulex (daphnia) ENSDARP00000028095 Danio rerio (zebra fish) ENSECAP00000012413 Equus caballus (horse) ENSGACP00000013283 Gymnopilus aculeatus (mushroom) ENSGALP00000028428 Gallus gallus (chicken) 112029 Hypsipyla robusta (cedar shoot borer) ENSP00000322898 Homo sapiens (human) ISCW021817-PA Ixodes scapularis (deer tick) 225788 Lottia gigantea (owl limpet) ENSMODP00000010194 Monodelphis domestica (gray opossum) ENSMMUP00000015017 Macaca mulatta (rhesus monkey) ENSMUSP00000099857 Mus musculus (house mouse) 164975 Nematostella vectensis (starlet anemone) XP_001607605.1 Nasonia vitripennis (wasp) ENSOANP00000019523 Ornithorynchus anatinus (platypus) ENSORLP00000025300 Oryzias latipes (japanese killifish) PHUM006823-PA Pediculus humanus (human body louse) ENSPPYP00000017900 Pongo pygmaeus (orangutan) ENSPTRP00000029857 Pan troglodytes (chimpanzee) ENSRNOP00000050344 Rattus norvegicus (norway rat) XP_001185868.1 Strongylocentrotus purpuratus (purple sea urchin) 28406 Trichoplax adhaerens (metazoa) XP_973686.2 Tribolium castaneum (red flour beetle) ENSTNIP00000018930 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000013898 Takifugu rubripes (pufferfish Takifugu) ENSXETP00000024690 Xenopus tropicalis (frog) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV10306 LH17960 ISCW021817 FBgn0230042 FBgn0225257 FBgn0209060 FBgn0196941 FBgn0175065 JGI_V11_22285 FBgn0245832 FBgn0148266 FBgn0143754 FBgn0127942 FBgn0120434 FBgn0114569 FBgn0088818 CPIJ010142 BGIBMGA011565 ACYPI007722 GB14092 AGAP011437 AAEL003436 PB25446 PHUM119360 GLEAN_01270
Stocks & Reagents
Stocks Listed in FlyBase ( 11 )
Bloomington	316 kn1 9551 w*; P{UAS-kn.M}15+9/SM1 31916 y1 v1; P{TRiP.JF02206}attP2 35112 y1 w*; Mi{MIC}knMI02387 17588 y1 w67c23; P{EPgy2}knEY09641/CyO 9550 y1 w*; P{FRT(whs)}G13 knKN4/CyO, y+ 9574 y1 w*; T(1;2)knSA2, Tp(1;2)TE21F22A, knSA2/CyO
Kyoto	105853 kn1 109022 w*; kncol-1/CyO 109023 w*; kncol-1 / CyO; P{col5-cDNA.C} / TM6B
VDRC	v103837 P{KK114322}VIE-260B
Genomic Clones ( 1 )
	BACR03K23 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 38 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	RE03728
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	LP01538 RE29885 RE37371 RE71320 RE71366 RH09628
Other clones	35309 79394 102015 282419 CK01.76C.G11 CK01.77C.B12 CK01.77C.D11 CK01.86B.A2 CK01.92A.A6 CK01.102B.G1 EK059921 EK060416 EK060540 EK068133 EK071935 EK146441 EK225216 EK308146 RP004160971 RP004197458 RP004248059 RP004250178 RP004415877 RP004416515 RP004421517 RP004463221 RP004494327 RP004499452 RP004510863 RP004513340 RP004531072
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0001319 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 45318
Antibody Information
	polyclonal (Jinushi-Nakao et al., 2007)
Other Information
Discoverer
	Nichols-Skoog, 1st Aug. 1931.
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: kn CG10197 (Bayraktaroglu, 2000.4.3) Source for merge of: kn col (Vervoort et al., 1999)
Additional comments
Other Comments
	Ectopic expression of kn increases arbor length and represses filopodia/spike formation in class III neurons. (Jinushi-Nakao et al., 2007) 10 alleles of kn have been recovered in a screen for mutations with smo-like mutant phenotypes in clones in the wing. (Vegh and Basler, 2003) kn is required for the proper positioning of veins 3 and 4 in the wing and to prevent ectopic venation between them. (Mohler et al., 2000) kn is required for the formation of the hypopharyngeal lobe and for subsequent differentiation of the ventral arms and lateralgrate of the pharyngeal head skeleton. (Seecoomar et al., 2000) kn shows lineage-specific restriction of transcription to the founder of muscle DA3A during muscle development, which depends on the asymmetric segregation of numb gene product and involves repression by N signalling. (Crozatier and Vincent, 1999) In kn mutant embryos the DA3A founder cells form but do not maintain kn transcription and are unable to fuse with neighboring myoblasts, leading to a loss-of-muscle DA3A phenotype. (Crozatier and Vincent, 1999) The specification of the DA3A muscle lineage requires both kn and at least one other transcription factor. (Crozatier and Vincent, 1999) kn is required for the establishment of the PS(-1)/PS0 parasegmental border and formation of the intercalary segment. (Crozatier et al., 1999) kn is a mediator of the short range organizing activity of hh in the wing. (Vervoort et al., 1999) kn has a specific role in hh-mediated patterning of the wing with no appreciable effect on general dpp-mediated wing organisation. kn acts in the hh-responsive cells at the posterior edge of the anterior compartment of the wing, primarily by preventing vein formation by these cells, thus forming a distinct intervein region between veins 3 and 4. (Nestoras et al., 1997) Mutants display hyperplastic phenotype, showing tissue overgrowth in mitotic recombination clones. (Mechler, 1994) Mutations in kn cause extra longitudinal or transverse veins with plexated vein phenotypes. kn, fu and shf act synergistically against Px, px, net, dsr and emc. (Diaz-Benjumea and Garcia-Bellido, 1990) The kn and fu loci belong to the knot phenotypic group within the 'excess-of-vein' mutant class. Mutants generate a medial shift in the position of the longitudinal veins, sensilla and chaetae. Double mutants are superadditive in vein pattern defects. (Diaz-Benjumea et al., 1989)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_080074 NM_137146 NM_166070 NM_001103847 RefSeq (Proteins) • NP_524813 NP_610990 NP_725419 NP_001097317 DDBJ / EMBL / GenBank • AAF58203 AAF58204 AAM50962 AAM68551 ABV53800 AY119102 BI164134 CL526130 X97803 Entrez Gene - A searchable database of RefSeq genes. • 45318 UniProtKB/Swiss-Prot • P56721
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Transcription factor COE, conserved site (IPR018350) Cell surface receptor IPT/TIG (IPR002909) Transcription factor COE (IPR003523) Immunoglobulin E-set (IPR014756) Helix-loop-helix DNA-binding (IPR011598) Immunoglobulin-like fold (IPR013783)
Linkouts
	BioGRID - A database of protein and genetic interactions • 69588 DroID - A comprehensive database of gene and protein interactions. • FBgn0001319 DRSC - Results from RNAi screens. • FBgn0001319 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0001319 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • HDC06452;CG10197-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0001319 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 45318 InParanoid A subset of ortholog calls from InParanoid. • AAEL003436-PA Aedes aegypti (mosquito) AGAP011437-PA Anopheles gambiae (mosquito) XP_394171.3 Apis mellifera (honey bee) XP_001946640.1 Acyrthosiphon pisum (pea aphid) ENSBTAP00000042518 Bos taurus (bovine) CN16160 Caenorhabditis brenneri (small nematode) CBP33933 Caenorhabditis briggsae (nematode) CE29366 Caenorhabditis elegans (worm) ENSCAFP00000009901 Canis familiaris (dog) ENSCINP00000006573 Ciona intestinalis (sea squirt) JA13194 Caenorhabditis japonica (nematode) CPIJ010142 Culex pipens (mosquito) ENSCPOP00000018411 Cavia porcellus (guinea pig) RP27403 Caenorhabditis remanei (nematode) ENSCSAVP00000000810 Ciona savignyi (sedentary tunicate) 93575 Capitella spl (polychaete worm) 22285 Daphnia pulex (daphnia) ENSDARP00000028095 Danio rerio (zebra fish) ENSECAP00000012413 Equus caballus (horse) ENSGACP00000013283 Gymnopilus aculeatus (mushroom) ENSGALP00000028428 Gallus gallus (chicken) 112029 Hypsipyla robusta (cedar shoot borer) ENSP00000322898 Homo sapiens (human) ISCW021817-PA Ixodes scapularis (deer tick) 225788 Lottia gigantea (owl limpet) ENSMODP00000010194 Monodelphis domestica (gray opossum) ENSMMUP00000015017 Macaca mulatta (rhesus monkey) ENSMUSP00000099857 Mus musculus (house mouse) 164975 Nematostella vectensis (starlet anemone) XP_001607605.1 Nasonia vitripennis (wasp) ENSOANP00000019523 Ornithorynchus anatinus (platypus) ENSORLP00000025300 Oryzias latipes (japanese killifish) PHUM006823-PA Pediculus humanus (human body louse) ENSPPYP00000017900 Pongo pygmaeus (orangutan) ENSPTRP00000029857 Pan troglodytes (chimpanzee) ENSRNOP00000050344 Rattus norvegicus (norway rat) XP_001185868.1 Strongylocentrotus purpuratus (purple sea urchin) 28406 Trichoplax adhaerens (metazoa) XP_973686.2 Tribolium castaneum (red flour beetle) ENSTNIP00000018930 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000013898 Takifugu rubripes (pufferfish Takifugu) ENSXETP00000024690 Xenopus tropicalis (frog) Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /gene/collier1.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 45318 modMine - Data generated by the modENCODE project. • FBgn0001319 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV10306 LH17960 ISCW021817 FBgn0230042 FBgn0225257 FBgn0209060 FBgn0196941 FBgn0175065 JGI_V11_22285 FBgn0245832 FBgn0148266 FBgn0143754 FBgn0127942 FBgn0120434 FBgn0114569 FBgn0088818 CPIJ010142 BGIBMGA011565 ACYPI007722 GB14092 AGAP011437 AAEL003436 PB25446 PHUM119360 GLEAN_01270 REDfly - A database of transcriptional regulatory elements. • FBgn0001319
Synonyms & Secondary IDs ( 14 )
Reported As
Symbol Synonym	CG10197 (Klebes et al., 2005, Mohit et al., 2006, Keleman et al., 2009.8.5, Perkins et al., 2009) Col (Torroja et al., 2004, Daoud and Blanchet-Tournier, 2005, Briscoe, 2004, Anonymous, 2005, Apionishev et al., 2005, Jia et al., 2004, Perrin et al., 2004, Orgogozo et al., 2004, Vincent et al., 2004, Han et al., 2004, Crozatier et al., 2003, Dubois and Vincent, 2001, Crozatier et al., 2001, Crozatier et al., 2001, Crozatier and Vincent, 1999, Crozatier et al., 2005, Du et al., 2011, Hattori et al., 2007, Katanaev et al., 2008, Márkus et al., 2009, Dawber et al., 2005, Nahmad and Stathopoulos, 2009, Enriquez et al., 2010, Cheng et al., 2010, Yan et al., 2010, Wojcinski et al., 2011) col (Panakova et al., 2005, Crozatier et al., 2004, Crozatier et al., 2004, Casali and Struhl, 2004, Rajewsky and Socci, 2004, Ogden et al., 2004, Hooper, 2003, Crozatier et al., 2003, Vegh and Basler, 2003, Kambris et al., 2002, Glise et al., 2002, Tallafuss and Bally-Cuif, 2002, Amanai and Jiang, 2001, Johnson et al., 2002, Ingham and McMahon, 2001, Haas et al., 2001, Capdevila and Johnson, 2000, Johnson et al., 2000, Vervoort, 2000, Crozatier et al., 1999, Vervoort et al., 1999, Crozatier and Vincent, 1999, Son, 1999.3.8, Chang et al., 1999, Vervoort et al., 1998, Crozatier et al., 1998, Dubois et al., 1998, Crozatier et al., 1997, Crozatier et al., 1996, Vincent, 1995, Gorfinkiel et al., 2005, Baumgardt et al., 2007, Bartscherer et al., 2006, Dubois et al., 2007, Mandal et al., 2007, Krzemien et al., 2007, Jones, 2007, Baumgardt et al., 2009, Wendler et al., 2006, Baumgardt et al., 2008, Enriquez et al., 2008, Seong et al., 2010, Torroja et al., 2005, Ruel et al., 2007, Dubois et al., 2007, Claret et al., 2007, Crozatier and Vincent, 2008, Cheng et al., 2010, Mosimann et al., 2009, Tokusumi et al., 2009, Glise et al., 2005, Karlsson et al., 2010, Losada-Pérez et al., 2010) Col/Kn (Crozatier and Vincent, 2008) collier (Govind and Nehm, 2004) Kn (Crozatier et al., 2001, Molnar and de Celis, 2006, Ye et al., 2011, Shimono et al., 2009, Molnar et al., 2007) kn (Ramos and Mohler, 2006, Gilbert et al., 2006, Ramos et al., 2006, Mohit et al., 2006, Christensen et al., 2008.4.15, Hattori et al., 2007, Seong et al., 2010, Sandmann et al., 2006, Choksi et al., 2006, Jinushi-Nakao et al., 2007, Liu et al., 2009, Economou and Telford, 2009, Hurlbut et al., 2009, Christensen et al., 2009.7.22, Mosimann et al., 2009, Zeitlinger et al., 2007, Glise et al., 2005, Long et al., 2009, Usha and Shashidhara, 2010, Beltran et al., 2007, The modENCODE Consortium, 2010, The modENCODE Consortium, 2010)
Name Synonym	collier (Mosimann et al., 2009, Peel, 2004, Urbach and Technau, 2004, Baylies and Michelson, 2001, Potter and Xu, 2001, Martin et al., 2001, Ledent and Vervoort, 2001, Bier, 2000, Crozatier, 1996.5.13, Frasch, 1999, Crozatier et al., 2004, Ogden et al., 2004, Vervoort, 2000, Baumgardt et al., 2007, Baumgardt et al., 2007, Bartscherer et al., 2006, Dubois et al., 2007, Jones, 2007, Baumgardt et al., 2009, Du et al., 2011, Wendler et al., 2006, Ulvklo et al., 2008, Hattori et al., 2007, Torroja et al., 2005, Callejo et al., 2006, Eugster et al., 2007, Ruel et al., 2007, Dubois et al., 2007, Claret et al., 2007, Márkus et al., 2009, Cheng et al., 2010, Colomb et al., 2008, Usha and Shashidhara, 2010) Collier (Torroja et al., 2004, Anonymous, 2005, Orgogozo and Schweisguth, 2004, Crozatier et al., 2003, Dubois and Vincent, 2001, Crozatier et al., 2001, Crozatier et al., 2005, Vincent et al., 2007, Enriquez et al., 2008, Beckett and Baylies, 2007, Crozatier and Vincent, 2008, Orgogozo and Grueber, 2005, Dawber et al., 2005, Tokusumi et al., 2009, Shimono et al., 2009, Nahmad and Stathopoulos, 2009, Cheng et al., 2010, Yan et al., 2010, Losada-Pérez et al., 2010, Wojcinski et al., 2011) collier/knot (Nilsson et al., 2007, Karlsson et al., 2010) Collier/Knot (Crozatier and Vincent, 2008) Collier-Knot (Miller et al., 2003) knot (Mosimann et al., 2009, Pearson et al., 2005, Ulvklo et al., 2008, Economou and Telford, 2009, Hurlbut et al., 2009, Usha and Shashidhara, 2010) Knot (Crozatier et al., 2001, Dubois et al., 2007, Ye et al., 2011, Hattori et al., 2007, Long et al., 2009, Shimono et al., 2009, Molnar et al., 2007, Losada-Pérez et al., 2010) knotted (Miller and Blair, 1997, Miller and Blair, 1997)
Secondary FlyBase IDs
	FBgn0014142 FBgn0033967
References ( 175 )
Generate a list of	All references relating to this gene ( 175 )
List References by type	Research paper  ( 98 ) Supplementary material  ( 6 ) Review  ( 24 ) Personal communication to FlyBase  ( 10 ) Abstract  ( 29 ) FlyBase analysis  ( 1 ) DNA/RNA sequence record  ( 1 ) Protein sequence record  ( 1 ) Computer file  ( 2 ) Book  ( 1 ) Curated genome annotation  ( 1 )
Recent research papers ( 14 )
	Benito-Sipos et al., 2011, Development 138(24): 5311--5320 Seven up acts as a temporal factor during two different stages of neuroblast 5-6 development. [FBrf0216799] Demilly et al., 2011, PLoS ONE 6(6): e21213 Coe genes are expressed in differentiating neurons in the central nervous system of protostomes. [FBrf0214030] Du et al., 2011, PLoS ONE 6(9): e24168 In Vivo RNAi Screen Reveals Neddylation Genes as Novel Regulators of Hedgehog Signaling. [FBrf0215574] Gabilondo et al., 2011, Mech. Dev. 128(3-4): 208--221 A targeted genetic screen identifies crucial players in the specification of the Drosophila abdominal Capaergic neurons. [FBrf0213290] Quan et al., 2011, Nat. Biotechnol. 29(5): 449--452 Parallel on-chip gene synthesis and application to optimization of protein expression. [FBrf0213642] Wojcinski et al., 2011, Dev. Biol. 358(1): 168--180 DSulfatase-1 fine-tunes Hedgehog patterning activity through a novel regulatory feedback loop. [FBrf0215253] Ye et al., 2011, J. Neurosci. 31(9): 3309--3319 Differential regulation of dendritic and axonal development by the novel kruppel-like factor dar1. [FBrf0213162] Cheng et al., 2010, Dev. Biol. 337(1): 99--109 Regulation of smoothened by Drosophila G-protein-coupled receptor kinases. [FBrf0209432] Enriquez et al., 2010, Development 137(3): 457--466 Multi-step control of muscle diversity by Hox proteins in the Drosophila embryo. [FBrf0209729] Karlsson et al., 2010, PLoS Biol. 8(5): e1000368 Segment-specific neuronal subtype specification by the integration of anteroposterior and temporal cues. [FBrf0210822] Losada-Pérez et al., 2010, Mech. Dev. 127(9-12): 458--471 Lineage-unrelated neurons generated in different temporal windows and expressing different combinatorial codes can converge in the activation of the same terminal differentiation gene. [FBrf0212045] Seong et al., 2010, PLoS ONE 5(12): e15365 Inhibition of the nuclear import of cubitus interruptus by roadkill in the presence of strong hedgehog signal. [FBrf0212658] Usha and Shashidhara, 2010, Dev. Biol. 341(2): 389--399 Interaction between Ataxin-2 Binding Protein 1 and Cubitus-interruptus during wing development in Drosophila. [FBrf0210647] Yan et al., 2010, Development 137(12): 2033--2044 The cell-surface proteins Dally-like and Ihog differentially regulate Hedgehog signaling strength and range during development. [FBrf0210906] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...