knirps-related, knirps related
Please see the JBrowse view of Dmel\knrl for information on other features
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Gene model reviewed during 5.45
Gene model reviewed during 6.02
3.8 (northern blot)
647 (aa)
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\knrl using the Feature Mapper tool.
Comment: rectum and small intestine primordia
Comment: two lateral cells rows on either side of embryonic large intestine
Comment: anlage in statu nascendi
Comment: anlage in statu nascendi
Comment: anlage in statu nascendi
Comment: reported as procephalic ectoderm anlage in statu nascendi
Comment: reported as procephalic ectoderm anlage in statu nascendi
Comment: reported as procephalic ectoderm anlage in statu nascendi
Comment: reported as procephalic ectoderm anlage
Comment: reported as procephalic ectoderm anlage
Comment: reported as procephalic ectoderm anlage
Comment: reported as procephalic ectoderm anlage
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: reported as adult hindgut specific anlage
Comment: reported as rectum specific anlage
knrl is expressed in the third instar wing disc in a narrow stripe corresponding to the position of the L2 primordium.
The knrl transcript is expressed at low levels from 0-3 hours of embryogenesis. Expression levels increase in late embryogenesis and in larvae and adults. In situ hybridization experiments show that, from egg deposition until embryonic cycle 8, the knrl transcript is ubiquitous in the embryo. At embryonic cycle 12, expression is detected at 80-100% egg length in a ventral and anterior region. This expression intensifies at the cellular blastoderm stage, and additional intense expression appears at about 70% and 25% egg length. The e pression at 70% egg length is more intense ventrally than dorsally.
GBrowse - Visual display of RNA-Seq signals
View Dmel\knrl in GBrowse 23-47
3-47
3-43.7
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
No loss-of-function alleles have been isolated.
Both Dpp and FGF pathways control knrl expression.
knrl and kni possess multiple and redundant functions during tracheal development. knrl/ kni activity is necessary to mediate dpp signalling (which is required for tracheal cell migration and formation of dorsal and ventral branches). knrl/ kni activity in dorsal tracheal cells is essential for secondary and terminal branch formation, via repression of salm.
The biochemically equivalent gene products of kni and knrl are both functional in the head anlage and lack of one gene can be overcome by the activity of the other. kni is also required for abdominal segmentation and knrl is nonfunctional in its posterior expression domain. Therefore the kni/knrl pair of genes provides a region-specific buffering system, rather than global functional redundancy.
knrl differs from kni with respect to transcrition unit size, kni contains 1kb and knrl contains 19kb intron sequences. The consequence of the intron difference is that knrl cannot substitute for kni segmentation function. The length of the mitotic cycle provides a physiological barrier to transcript size and could be a significant factor in controlling developmental gene activity during short phenocritical periods.
Identification: Isolated from a genomic library using a Drosophila kni zinc-finger probe.
Identification: Isolated from a genomic library using a human retinoic acid receptor cDNA probe.