is expressed in Malpighian tubule Type II (stellate) cells in early stage 13 embryos, just after they have pushed into the renal epithelium. All Malpighian tubule Type II cells contact the luminal surface of the tubules in mid stage 14 and express high levels of dlg1
as well as crb
, and baz
is expressed in central brain neuropils. Lamina cross-sections exhibited a bright ring of R1-R6 terminals that label with dlg1
. Using immunoelectron microscopy, dlg1
is often seen close to the heads of the capitate projections (invaginations from surrounding epithelial glial and specialised organelles for the endocytosis of vesicle membrane), but not the stalks.
protein is found mainly in epithelia, where it is localized at the apical part of the lateral cell membrane.
Expression of dlg1
is observed in the neuromuscular junction of wildtype fly at 3, 15, 30 and 60 days post-eclosion.
In the adult prothorax and neck, dlg1
immunoreactivity is observed in the type I boutons of motor neurons innervating ventral cervical muscles, prothoracic sternal anterior rotator muscle 31, and prothoracic sternal adductor muscle 33. dlg1
protein is localized to all prothoracic type I nerve terminals. Extrasynaptic dlg1
immunoreactivity is also observed in all adult muscles in a highly regular pattern resembling a reticular network.
In male reproductive tissue, dlg1
protein is found in the apical region of the lateral membranes of the accessory gland and the ejaculatory bulb. A complex pattern of staining is seen also in the testis. In the ovary, staining is observed in lateral membranes of follicle cells and in the cytoplasm of nurse cells and the oocyte. In cellular blastoderm embryos, dlg1
protein is found at the apical region of the forming lateral cell membrane. It is around each cell but not at the corners. At the time of dorsal closure, staining in the epidermis is restricted along the cell boundaries. Staining is seen in peripheral neurons, in gut, and in axon bundles. In larvae, dlg1
protein is found in salivary glands, proventriculus, imaginal discs, and CNS.