FlyBase Gene Report: Dmel\eIF4A

General Information

Symbol

Dmel\eIF4A

Species

D. melanogaster

Name

eukaryotic translation initiation factor 4A

Annotation Symbol

CG9075

Feature Type

protein_coding_gene

FlyBase ID

FBgn0001942

Gene Model Status

Current

Stock Availability

22 publicly available

Gene Snapshot

eukaryotic translation initiation factor 4A (eIF4A) encodes an essential DEAD-box RNA helicase protein and a canonical translation initiation factor. It is a component of the eIF4F cap-binding complex that is essential for cap-dependent translation of mRNA. [Date last reviewed: 2019-03-07]

Other Summaries

Alliance
Auto summary
Pathway
Gene Group
UniProtKB
Interactive Fly

Also Known As

eIF-4a, l(2)gdh-4, l(2L)162, deIF4A, l(2)k01501

Key Links

Genomic Location

Cytogenetic map

26B2-26B2

Sequence location

2L:5,981,764..5,985,909 [+]

Recombination map

2-18

RefSeq locus

NT_033779 REGION:5981764..5985909

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (18 terms)

Molecular Function (4 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables SMAD binding

inferred from physical interaction with FLYBASE:Mad; FB:FBgn0011648

(Li and Li, 2006)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

enables ATP binding

inferred from electronic annotation with InterPro:IPR011545

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

enables RNA helicase activity

inferred from sequence or structural similarity with SGD:S000003674

(Lasko, 2000)

enables translation initiation factor activity

inferred from biological aspect of ancestor with PANTHER:PTN002777147

(assigned by GO_Central )

(Gaudet et al., 2011)

inferred from sequence or structural similarity

(Lasko, 2000)

Biological Process (10 terms)

Terms Based on Experimental Evidence (8 terms)

CV Term

Evidence

References

involved_in female germ-line stem cell asymmetric division

inferred from mutant phenotype

(Shen et al., 2009)

inferred from genetic interaction with FLYBASE:bam; FB:FBgn0000158

(Shen et al., 2009)

involved_in germ-line stem cell population maintenance

inferred from mutant phenotype

(Shen et al., 2009)

involved_in mitotic cell cycle

inferred from high throughput mutant phenotype

(Ducat et al., 2008)

involved_in negative regulation of BMP signaling pathway

inferred from direct assay

(Li and Li, 2006)

inferred from mutant phenotype

(Li and Li, 2006)

involved_in oogenesis

inferred from mutant phenotype

(Jagut et al., 2013)

inferred from genetic interaction with FLYBASE:bam; FB:FBgn0000158

(Shen et al., 2009)

involved_in pole cell formation

inferred from genetic interaction with FLYBASE:tud; FB:FBgn0003891

(Thomson et al., 2008)

inferred from genetic interaction with FLYBASE:vas; FB:FBgn0283442

(Thomson et al., 2008)

involved_in positive regulation of proteasomal ubiquitin-dependent protein catabolic process

inferred from direct assay

(Li and Li, 2006)

involved_in regulation of alternative mRNA splicing, via spliceosome

inferred from high throughput mutant phenotype

(Park et al., 2004)

inferred from mutant phenotype

(Katzenberger et al., 2009)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in cytoplasmic translational initiation

inferred from biological aspect of ancestor with PANTHER:PTN002777147

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in translational initiation

inferred from sequence or structural similarity

(Lasko, 2000)

Cellular Component (4 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

located_in cytosol

inferred from direct assay

(Li and Li, 2006)

located_in nucleus

inferred from high throughput direct assay

(Lee et al., 2014)

located_in P granule

inferred from direct assay

(Thomson et al., 2008)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

located_in cytosol

inferred from sequence or structural similarity

(Lasko, 2000)

part_of eukaryotic translation initiation factor 4F complex

inferred from sequence or structural similarity

(Lasko, 2000)

located_in nucleus

inferred by curator from GO:0000381

(Park et al., 2004)

Gene Group (FlyBase)

CYTOPLASMIC TRANSLATION INITIATION FACTORS

DEAD-BOX RNA HELICASES

Pathway (FlyBase)

Negative Regulators of BMP Signaling Pathway

Protein Family (UniProt)

Belongs to the DEAD box helicase family. eIF4A subfamily. (Q02748)

Protein Signatures (InterPro)

Summaries

Gene Group (FlyBase)

CYTOPLASMIC TRANSLATION INITIATION FACTORS -: Cytoplasmic (or eukaryotic) translation initiation factors function in the initiation stage of ribosome-mediated translation in the cytoplasm. (Adapted from FBrf0233552 and PMID:20094052).
DEAD-BOX RNA HELICASES -: DEAD-box RNA helicases belong to helicase superfamily 2 (SF2). They are characterized by the presence of nine conserved helicase motifs, including the DEAD sequence in motif II. (Adapted from PMID:21509200.)

Pathway (FlyBase)

Negative Regulators of BMP Signaling Pathway -: Negative regulators of Bone Morphogenetic Protein (BMP) signaling down-regulate the pathway, ultimately resulting in the decreased nuclear activity of the Mad/Med transcription factor complex.

Protein Function (UniProtKB)

ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. Involved in germ cell formation.

(UniProt, Q02748)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\eIF4A for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.55

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.44

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

eIF4A-RA

FBtr0079175

NM_164668

1691

403

eIF4A-RB

FBtr0079177

NM_164669

1847

403

eIF4A-RC

FBtr0079176

NM_057247

1553

403

eIF4A-RD

FBtr0079178

NM_164670

1960

403

eIF4A-RE

FBtr0307068

NM_001258978

2021

403

eIF4A-RF

FBtr0331201

NM_001273188

1939

403

Additional Transcript Data and Comments

Reported size (kB)

1.9, 1.75 (northern blot)

(Dorn et al., 1993)

2.2, 2.0 (northern blot)

(Cooley et al., 1992)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

eIF4A-PA

FBpp0078806

45.9

403

5.34

NP_723137

AAF52317

eIF4A-PB

FBpp0078808

45.9

403

5.34

NP_723138

AAN10567

eIF4A-PC

FBpp0078807

45.9

403

5.34

NP_476595

AAN10566

eIF4A-PD

FBpp0078809

45.9

403

5.34

NP_723139

AAN10568

eIF4A-PE

FBpp0297911

45.9

403

5.34

NP_001245907

AFH03581

eIF4A-PF

FBpp0303628

45.9

403

5.34

NP_001260117

AGB92653

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

403 aa isoforms: eIF4A-PA, eIF4A-PB, eIF4A-PC, eIF4A-PD, eIF4A-PE, eIF4A-PF

Additional Polypeptide Data and Comments

Reported size (kDa)

402 (aa)

(Dorn et al., 1993)

Comments

External Data

Subunit Structure (UniProtKB)

eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least eIF4A, eIF4E1 and eIF4G1. Interacts with tud and vas.

(UniProt, Q02748)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\eIF4A using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 12

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 13 -- 16

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage

organism | ubiquitous | uniform

(Hernandez et al., 2004)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

organism | ubiquitous

(Dorn et al., 1993)

embryonic stage -- adult stage

(Dorn et al., 1993)

organism

(Hernandez et al., 2004)

Additional Descriptive Data

eIF-4a transcripts are detected at all developmental stages. In embryos, they are already present in large amounts in fertilized eggs and are abundant in all tissues at later stages of development.

(Dorn et al., 1993)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

organism | ubiquitous | uniform

(Hernandez et al., 2004)

embryonic stage 5

organism

• apical cortex

(Hernandez et al., 2004)

third instar larval stage

wing disc | ubiquitous | uniform

(Hernandez et al., 2004)

haltere disc | ubiquitous | uniform

(Hernandez et al., 2004)

ventral thoracic disc | ubiquitous | uniform

(Hernandez et al., 2004)

eye-antennal disc | ubiquitous | uniform

(Hernandez et al., 2004)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

eIF-4a protein is ubiquitously distributed at all embryonic stages. In cellular blastoderms, protein is significantly accumulated in the apical cortex of blastoderm cells. eIF-4a protein is evenly distributed in wing, haltere, leg, and eye-antennal discs.

(Hernandez et al., 2004)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in cytosol

inferred from direct assay

(Li and Li, 2006)

located_in nucleus

inferred from high throughput direct assay

(Lee et al., 2014)

located_in P granule

inferred from direct assay

(Thomson et al., 2008)

Expression Deduced from Reporters

Reporter: P{eIF4A-lacZ.-436wt}

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

embryonic/larval salivary gland

(Ida et al., 2007)

eye-antennal disc | restricted

(Ida et al., 2007)

embryonic/larval brain | restricted

(Ida et al., 2007)

spermatogenesis & adult stage | male

testis | restricted | variable

(Ida et al., 2007)

adult stage | female & oogenesis

female germline cell

(Ida et al., 2007)

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\eIF4A in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\eIF4A

View exonic expression by tissue for Dmel\eIF4A

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0001942

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0001942

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG9075-RA

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG9075

Flygut - An atlas of the Drosophila adult midgut

FBgn0001942

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, and Transgenic Constructs

Classical and Insertion Alleles ( 63 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 13 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of eIF4A

Transgenic constructs containing regulatory region of eIF4A

Deletions and Duplications ( 27 )

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal

lethal, with Scer\GAL4^nub-AC-62

eIF4A^HMS00927

lethal, with Scer\GAL4^pnr-MD237

eIF4A^GD14111

lethal (with Df(2L)Gpdh1-A)

eIF4A^YE9

lethal | embryonic stage (with Df(2L)E110)

eIF4A^YE9

lethal | female limited, with Scer\GAL4^dome-PG14

eIF4A^HMS00927

lethal | first instar larval stage (with Df(2L)E110)

eIF4A^YE9

lethal | first instar larval stage (with eIF4A⁰²⁴³⁹)

eIF4A^YE9

lethal | first instar larval stage (with eIF4A^YE9)

eIF4A⁰²⁴³⁹

lethal | pupal stage, with Scer\GAL4^pnr-MD237

eIF4A^GD14111

lethal | second instar larval stage (with Df(2L)E110)

eIF4A⁰²⁴³⁹

lethal | second instar larval stage (with eIF4A⁰²⁴³⁹)

eIF4A^YE9

lethal | second instar larval stage (with eIF4A^YE9)

eIF4A⁰²⁴³⁹

lethal | third instar larval stage (with Df(2L)E110)

eIF4A⁰²⁴³⁹

lethal - all die before end of embryonic stage | recessive

eIF4A^YE9

lethal - all die before end of larval stage, with Scer\GAL4^en-e16E

eIF4A^KK107917

lethal - all die before end of larval stage | recessive

lethal - all die during larval stage, with Scer\GAL4^Mef2.PR

eIF4A^GD14111

partially lethal, with Scer\GAL4^tin.CΔ4

eIF4A^GD14111

partially lethal - majority die | recessive

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

eIF4A^GD14111

Other Phenotypes

Allele

decreased cell number | cell autonomous | larval stage | somatic clone

decreased cell number | germline clone

eIF4A¹⁰⁰⁶

decreased cell number | third instar larval stage, with Scer\GAL4^repo

eIF4A^GD14111

decreased occurrence of cell division | larval stage

developmental rate defective | dominant | larval stage

eIF4A^YE9

mitotic cell cycle defective | recessive

neuroanatomy defective, with Scer\GAL4^elav-C155

eIF4A^EP1011

neuroanatomy defective | third instar larval stage, with Scer\GAL4^repo

eIF4A^GD14111

some die during embryonic stage | recessive

eIF4A^YE9

some die during larval stage, with Scer\GAL4^tin.CΔ4

eIF4A^GD14111

some die during larval stage | recessive

Phenotype manifest in

Allele

amnioserosa

amnioserosa (with Df(2L)Gpdh1-A)

eIF4A¹⁰⁰⁶

anterior fascicle & synapse, with Scer\GAL4^elav-C155

eIF4A^EP1011

centrosome | germline clone

eIF4A^pecan-19.6

cephalopharyngeal skeleton

eIF4A^YE9

cuticle

egg chamber | germline clone

eIF4A^pecan-19.6

embryonic/first instar larval cuticle

eIF4A^YE9

embryonic/larval optic stalk | third instar larval stage, with Scer\GAL4^repo

eIF4A^GD14111

embryonic/larval salivary gland, with Scer\GAL4^fkh.PH

eIF4A^EP1011

eye

eye disc | third instar larval stage, with Scer\GAL4^repo

eIF4A^GD14111

female germline stem cell | germline clone

glial cell | third instar larval stage, with Scer\GAL4^repo

eIF4A^GD14111

head

intersegmental nerve, with Scer\GAL4^elav-C155

eIF4A^EP1011

oocyte | germline clone

eIF4A^pecan-19.6

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (5)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\EIF4A1

12 of 15

Yes

Hsap\EIF4A2

12 of 15

Yes

Hsap\EIF4A3

2 of 15

Hsap\CDK2

1 of 15

Hsap\MIR1248

1 of 15

Yes

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (4)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Eif4a1

12 of 15

Yes

Mmus\Eif4a2

12 of 15

Yes

Mmus\Eif4a3

2 of 15

Mmus\Gm8994

1 of 15

Rattus norvegicus (Norway rat) (3)

Rnor\Eif4a2

11 of 13

Yes

Rnor\Eif4a1

10 of 13

Yes

Rnor\Eif4a3

2 of 13

Xenopus tropicalis (Western clawed frog) (3)

Xtro\eif4a1

11 of 12

Yes

Xtro\eif4a2

10 of 12

Yes

Xtro\eif4a3

2 of 12

Danio rerio (Zebrafish) (4)

Drer\eif4a1a

10 of 15

Yes

Drer\eif4a1b

10 of 15

Yes

Drer\eif4a2

10 of 15

Yes

Drer\eif4a3

2 of 15

Caenorhabditis elegans (Nematode, roundworm) (4)

Cele\inf-1

13 of 15

Yes

Cele\F57B9.3

5 of 15

Yes

Cele\F33D11.10

2 of 15

Cele\Y65B4A.6

2 of 15

Arabidopsis thaliana (thale-cress) (6)

Atha\EIF4A1

8 of 9

Yes

Atha\EIF4A-2

8 of 9

Yes

Atha\AT1G72730

7 of 9

Yes

Atha\AT1G51380

2 of 9

Atha\AT2G28600

1 of 9

Yes

Atha\EIF4A-III

1 of 9

Saccharomyces cerevisiae (Brewer's yeast) (3)

Scer\TIF1

9 of 15

Yes

Scer\FAL1

4 of 15

Scer\TIF2

4 of 15

Yes

Schizosaccharomyces pombe (Fission yeast) (2)

Spom\SPAC1006.07

8 of 12

Yes

Spom\SPAC1F5.10

1 of 12

Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG0919097Y )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA21521

Drosophila persimilis

Dper\GL19047

Drosophila willistoni

Dwil\GK14711

Drosophila virilis

Dvir\GJ15436

Drosophila mojavensis

Dmoj\GI17537

Drosophila grimshawi

Dgri\GH11018

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091505KD )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17020772

Musca domestica

House fly

Mdoa\17016929

Glossina morsitans

Tsetse fly

Gmor\GMOY002522

Glossina morsitans

Tsetse fly

Gmor\GMOY003287

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_05212

Mayetiola destructor

Hessian fly

Mdes\Mdes006258

Mayetiola destructor

Hessian fly

Mdes\Mdes010974

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL013359

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL014414

Anopheles darlingi

American malaria mosquito

Adar\ADAC007455

Anopheles darlingi

American malaria mosquito

Adar\ADAC004044

Anopheles gambiae

Malaria mosquito

Agam\AGAP009863

Anopheles gambiae

Malaria mosquito

Agam\AGAP003089

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ001547

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W05FL )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA003186

Bombyx mori

Silkmoth

Bmor\BGIBMGA004822

Danaus plexippus

Monarch butterfly

Dple\DPOGS214941-PA

Danaus plexippus

Monarch butterfly

Dple\DPOGS211201-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL015594

Heliconius melpomene

Postman butterfly

Hmel\HMEL012453

Apis florea

Little honeybee

Aflo\2451681364

Apis florea

Little honeybee

Aflo\2198071780

Apis mellifera

Western honey bee

Amel\LOC409866

Apis mellifera

Western honey bee

Amel\GB51710

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP12462

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP25120

Bombus terrestris

Buff-tailed bumblebee

Bter\15216784

Bombus terrestris

Buff-tailed bumblebee

Bter\15208217

Linepithema humile

Argentine ant

Lhum\LH11979

Linepithema humile

Argentine ant

Lhum\LH24849

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:001e3b

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:00103c

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG018524

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG003545

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_06329

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_04286

Tribolium castaneum

Red flour beetle

Tcas\TC014113

Tribolium castaneum

Red flour beetle

Tcas\TC009998

Pediculus humanus

Human body louse

Phum\PHUM148640

Pediculus humanus

Human body louse

Phum\PHUM597330

Rhodnius prolixus

Kissing bug

Rpro\RPRC006840

Rhodnius prolixus

Kissing bug

Rpro\RPRC002208

Cimex lectularius

Bed bug

Clec\CLEC005239

Cimex lectularius

Bed bug

Clec\CLEC008969

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI001585

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI002483

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_04200

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_04771

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X05BL )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR001486

Strigamia maritima

European centipede

Smar\SMAR009015

Strigamia maritima

European centipede

Smar\SMAR011862

Ixodes scapularis

Black-legged tick

Isca\ISCW015864

Ixodes scapularis

Black-legged tick

Isca\ISCW013399

Stegodyphus mimosarum

African social velvet spider

Smim\KFM68235.1

Stegodyphus mimosarum

African social velvet spider

Smim\KFM75183.1

Stegodyphus mimosarum

African social velvet spider

Smim\KFM78111.1

Stegodyphus mimosarum

African social velvet spider

Smim\KFM62051.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur03g09740

Tetranychus urticae

Two-spotted spider mite

Turt\tetur03g09690

Tetranychus urticae

Two-spotted spider mite

Turt\tetur21g02590

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_300157

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_104930

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_230663

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G07OI )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Eif4A

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Ddx48

Ciona intestinalis

Vase tunicate

Cint\LOC100177596

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000007818

Gallus gallus

Domestic chicken

Ggal\EIF4A2

Gallus gallus

Domestic chicken

Ggal\EIF4A3

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (31)

Gene Symbol

Score

Source

Alignment

4 of 10

abs

2 of 10

bel

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

Hlc

2 of 10

mahe

2 of 10

me31B

2 of 10

pit

2 of 10

Prp5

2 of 10

Rm62

2 of 10

Rs1

2 of 10

vas

2 of 10

mus301

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

EIF4A1; eukaryotic translation initiation factor 4A1

12 of 15

602641

EIF4A2; eukaryotic translation initiation factor 4A2

12 of 15

601102

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

eIF4A - Akt1

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

eIF4A - Mad

anti bait coimmunoprecipitation, western blot, pull down, anti tag coimmunoprecipitation, anti tag western blot

(Li and Li, 2006)

eIF4A - Med

anti tag coimmunoprecipitation, anti tag western blot

(Li and Li, 2006)

eIF4A - NAT1

anti tag coimmunoprecipitation, anti tag western blot, peptide massfingerprinting

(Tsokanos et al., 2016)

eIF4A - Pdcd4

pull down, western blot, anti bait coimmunoprecipitation, anti tag western blot

(Olson et al., 2013)

eIF4A - Pdk1

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

eIF4A - Pi3K92E

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

eIF4A - Raf

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

eIF4A - RagC-D

anti tag coimmunoprecipitation, anti tag western blot

(Tsokanos et al., 2016)

eIF4A - Rheb

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

eIF4A - S6k

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

eIF4A - S6kII

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

eIF4A - aub

anti tag coimmunoprecipitation, anti tag western blot, cross-linking study, Identification by mass spectrometry

(Ma et al., 2017, Zheng et al., 2016)

eIF4A - bam

anti tag coimmunoprecipitation, anti tag western blot, two hybrid

(Shen et al., 2009)

eIF4A - csw

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

eIF4A - eIF3b

anti tag coimmunoprecipitation, western blot

(Rode et al., 2018)

eIF4A - eIF4G1

two hybrid

(Hernandez et al., 2004)

eIF4A - gig

anti tag coimmunoprecipitation, Identification by mass spectrometry, anti tag western blot

(Tsokanos et al., 2016, Vinayagam et al., 2016)

eIF4A - raptor

anti tag coimmunoprecipitation, anti tag western blot

(Tsokanos et al., 2016)

eIF4A - rictor

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

eIF4A - sima

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

eIF4A - tud

anti tag coimmunoprecipitation, anti tag western blot, cross-linking study, Identification by mass spectrometry, anti bait coimmunoprecipitation

(Zheng et al., 2016, Thomson et al., 2008)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

eIF4A

suppressible

(Galloni and Edgar, 1999)

eIF4A

suppressible

E2f1

(Galloni and Edgar, 1999)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

bam

suppressible

eIF4A

(Shen et al., 2009)

bam

enhanceable

eIF4A

(Shen et al., 2009)

enhanceable

eIF4A

(Krupp et al., 2005)

suppressible

eIF4A

(Krupp et al., 2005)

suppressible

(Li et al., 2005)

suppressible

(Ida et al., 2009, Ida et al., 2007)

l(1)sc

enhanceable

eIF4A

(Rottgen et al., 1998)

enhanceable

eIF4A

(Rottgen et al., 1998)

Pdcd4

suppressible

eIF4A

(Cash and Andrews, 2012)

peb

suppressible

eIF4A

(Wilk et al., 2004)

Pten

suppressible

eIF4A

(Gao et al., 2000)

External Data

Subunit Structure (UniProtKB)

eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least eIF4A, eIF4E1 and eIF4G1. Interacts with tud and vas.

(UniProt, Q02748 )

Linkouts

BioGRID - A database of protein and genetic interactions.

60000

DroID - A comprehensive database of gene and protein interactions.

FBgn0001942

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

33835

MIST (genetic) - An integrated Molecular Interaction Database

eIF-4a

MIST (protein-protein) - An integrated Molecular Interaction Database

eIF-4a

Pathways

Signaling Pathways (FlyBase)

Negative Regulators of BMP Signaling Pathway -: Negative regulators of Bone Morphogenetic Protein (BMP) signaling down-regulate the pathway, ultimately resulting in the decreased nuclear activity of the Mad/Med transcription factor complex.

Metabolic Pathways

External Data

Linkouts

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

RNA transport

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-18

Cytogenetic map

26B2-26B2

Sequence location

2L:5,981,764..5,985,909 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

26B2-26B2

Limits computationally determined from genome sequence between P{EP}chic^EP713&P{EP}chic^EP1095 and P{lacW}Gef26^k13720

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

25D5-26D1

(Li and Gelbart, 2002)

26B1-26B2

(determined by in situ hybridisation)

(Spradling et al., 1999)

26A5-26A7

26A5--8 26A7--8 26A7--9 26A8--9 26B1--2 26B8--9

(BDGP Project Members, 1994-1999)

26A7-26A9

(determined by in situ hybridisation) 27A7--27A9 (determined by in situ hybridisation)

(Dorn et al., 1993)

Experimentally Determined Recombination Data

Location

2-18

(FlyBase, 2016)

2-19.2

(Comeron et al., 2012)

Left of (cM)

(Li et al., 2005)

Right of (cM)

(Li et al., 2005)

Notes

Stocks and Reagents

Stocks (22)

Bloomington

8647

w^*; P{lacW}eIF4A¹⁰¹³/CyO

50847

w^*; P{PTT-un}eIF4A^P02046/CyO

FlyORF

F001750

M{UAS-eIF4A.ORF}ZH-86Fb

F000979

M{UAS-eIF4A.ORF.3xHA}ZH-86Fb

Kyoto

123245

w¹¹¹⁸; P{RS3}eIF4A^CB-0582-3

123292

w¹¹¹⁸; P{RS3}eIF4A^CB-0707-3

124307

w¹¹¹⁸; P{RS3}eIF4A^UM-8048-3

125515

w¹¹¹⁸; P{RS5}eIF4A^5-HA-1998

VDRC

v100310

P{KK107917}VIE-260B

v42202

w¹¹¹⁸; P{GD14111}v42202

v318782

PBac{fTRG01170.sfGFP-TVPTBF}VK00033

v42201

w¹¹¹⁸; P{GD14111}v42201/CyO

More stocks available...

Genomic Clones (18)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (908)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

LD22268

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0001942

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0001942

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

33835

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Tsokanos et al., 2016, Fukaya et al., 2014, Olson et al., 2013, Hernandez et al., 2004)

monoclonal

(Na et al., 2016)

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: eIF4A eIF-4a

(Marygold et al., 2017)

Source for database merge of

Source for merge of: eIF-4a l(2)k01501

(Spradling et al., 1999)

Source for merge of: eIF-4a Su(dpp)YE9

(Li et al., 2005)

Additional comments

Other Comments

RNAi screen using dsRNA made from templates generated with primers directed against this gene results in aberrantly short, monopolar spindles when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.

(Goshima et al., 2007)

S2 cells transfected with dsRNA made from templates generated with primers directed against this gene show significantly lower levels of global translation within 2 days.

(Coelho et al., 2005)

RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R⁺ cells.

(Boutros et al., 2004)

Identification: identified in a genetic screen for suppressors of the dpp^H46 phenotype.

(Li and Gelbart, 2002)

Maternal and zygotic suppressor of dpp^H46. Homozygous embryonic lethal. Cuticle phenotypes of homozygous mutant embryos show dorsal-ventral patterning defects. eIF-4a can completely rescue the maternal-effect lethality caused by Mad, sax double mutants.

(Li and Gelbart, 2002)

Identification: One of a collection of genes identified with defective larval growth that extend larval life.

(Galloni and Edgar, 1999)

Identification: Enhancer trap expression pattern survey for loci expressed in the ring gland.

(Harvie et al., 1998)

Mutants isolated in a screen of the second chromosome identifying genes affecting disc morphology.

(Roch et al., 1998)

A single copy gene.

(Dorn et al., 1993)

1.6kb non-sex-specific transcript located close to Acp26Aa in the 26A region.

(Monsma and Wolfner, 1988)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 83 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

33835

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q02748

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

C9QP42

Other crossreferences

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0001942

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0001942

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG9075

Flygut - An atlas of the Drosophila adult midgut

FBgn0001942

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

33835

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG9075

modMine - A data warehouse for the modENCODE project

FBgn0001942

Linkouts

ApoDroso - Functional genomic database for photoreceptor development, survival and function

10506-eIF-4a[k01501]

BioGRID - A database of protein and genetic interactions.

60000

DPiM - Drosophila Protein interaction map

FBgn0001942

DroID - A comprehensive database of gene and protein interactions.

FBgn0001942

DRSC - Results frm RNAi screens

FBgn0001942

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0001942

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG9075-RA

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0001942

FlyMine - An integrated database for Drosophila genomics

FBgn0001942

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

33835

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

RNA transport

MIST (genetic) - An integrated Molecular Interaction Database

eIF-4a

MIST (protein-protein) - An integrated Molecular Interaction Database

eIF-4a

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Synonyms and Secondary IDs (40)

Reported As

Symbol Synonym

2439

(Harvie et al., 1998)

9075

(Ravi et al., 2009)

Dm-eIF4A

(Hernandez et al., 2004)

DmRH2

(Boudet et al., 2001)

EIF-4A

(Hernandez et al., 2004, Boudet et al., 2001)

EIF-4a

(He et al., 2014, Povellato et al., 2014)

EIF4A

(Cui et al., 2020, Linsalata et al., 2019, Sofola et al., 2007)

EiF4A

(Weinkove and Leevers, 2000)

Eif-4a

(Morin et al., 2001)

Eif4a

(Nguyen et al., 2016)

L(2L)162

(Hernandez et al., 2004)

Su(dpp)YE9

(Li and Gelbart, 2002)

anon-26Aa

anon26A

deIF4A

(Gareau et al., 2013, Garrey et al., 2010)

eIF 4A

(Ducat et al., 2008)

eIF-4A

(Chen et al., 2013, Kent et al., 2009, Fuller and Baker, 2007.6.28, Beller et al., 2006, Takahashi et al., 2005, van Steensel et al., 2001)

eIF-4a

eIF-4a-I

(Hernandez and Vazquez-Pianzola, 2005)

eIF4A-I

(Hernandez and Vazquez-Pianzola, 2005)

eIF4AI

(Palacios et al., 2004)

eIF4a

(Ji et al., 2017, Olesnicky et al., 2014, Djiane et al., 2013, Blanco et al., 2010, Abel et al., 2009, Insco et al., 2009)

eiF4A

(Jagut et al., 2013)

eif-4a

(Basu and Li, 1998)

eif4A

(Orian et al., 2003, Gao et al., 2000, Galloni and Edgar, 1999, Galloni and Edgar, 1999)

eif4a

(Sofola et al., 2007)

l(2)02439

l(2)162

l(2)26Ac

l(2)gdh-4

(Knipple and MacIntyre, 1984, Kotarski et al., 1983)

l(2)k01501

(BDGP Project Members, 1994-1999)

l(2L)162

(Dorn et al., 1993, Dorn, 1992.10.29)

pecan

(Jagut et al., 2013)

Name Synonyms

Eukaryotic initiation factor 4A

(Costello et al., 2009)

Eukaryotic initiation factor 4a

(Cammarato et al., 2011)

eukaryotic initiation factor 4A

(Jagut et al., 2013, Ida et al., 2007)

eukaryotic translation initiation factor 4A

(Kent et al., 2009, Li and Li, 2006)

translation initiation factor 4A

(Hernandez et al., 2004)

Secondary FlyBase IDs

FBgn0013406
FBgn0013523
FBgn0015864
FBgn0022247
FBgn0024969
FBgn0062252

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (198)

The following external sites may use different assemblies or annotations than FlyBase.

Report Sections