Citron, citron kinase, dck, cit, l(3)7m-62
Rho effector kinase functioning downstream of pebble - required for cytokinesis
Please see the JBrowse view of Dmel\sti for information on other features
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AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.45
Gene model reviewed during 5.55
None of the polypeptides share 100% sequence identity.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\sti using the Feature Mapper tool.
The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).
Comment: maternally deposited
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
JBrowse - Visual display of RNA-Seq signals
View Dmel\sti in JBrowse
3-38
3-32.6
3-36
3-36.8
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
New stable cell line derived from S2-unspecified : S2 cell lines coexpresing scra-GFP and wild type or kinase-dead versions of sti were generated.
New stable cell line derived from S2-unspecified : S2 cell lines were generated expressing various sti-GFP constructs.
When dsRNA constructs are made and transiently transfected into S2 cells in RNAi experiments, a decrease in the ratio of cells in prometaphase and metaphase versus the total number of mitotic cells and a whole range of mitotic abnormalities are seen.
sti is required for the terminal (post-furrowing) events of cytokinesis.
dsRNA directed against this gene causes defects in cytokinesis when tested in an RNAi screen in S2 cells.
RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a binucleation phenotype when assayed in Kc167 cells.
dsRNA made from templates generated with primers directed against this gene causes a phenotype in S2 cells. These cells display higher frequencies of telophases, abnormal membrane protrusions at the cleavage furrow and lack the "Fleming body", a proteinaceous matrix associated with the central spindle midzone.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in Kc167 and S2R+ cells: binucleate cells.
dsRNA made from templates generated with primers directed against this gene is tested in an RNAi screen for effects on actin-based lamella formation.
Area matching Drosophila EST AA539198.
All alleles have mitotic chromosomes (both sisters and heterologues) stuck together and show metaphase delay; "successful" anaphase fragments the chromosomes.
Source for merge of: cit CG10522
Source for merge of: sti cit
Source for merge of: cit anon-WO0118547.388
Source for merge of cit anon-WO0118547.388 was sequence comparison ( date:051113 ).
