General Information
Symbol
Dmel\lab
Species
D. melanogaster
Name
labial
Annotation Symbol
CG1264
Feature Type
FlyBase ID
FBgn0002522
Gene Model Status
Stock Availability
Gene Snapshot
Labial (lab) is the anterio-most expressed member of the Antennapedia complex (ANT-C), one of two Hox gene complexes. Members of the ANT-C control the identity of segments that contribute to the head and the anterior thorax. Lab specifies the identity of the intercalary segment and also functions in the development of copper cells in the midgut. [Date last reviewed: 2016-06-30]
Also Known As
F90-2, F24
Genomic Location
Cytogenetic map
Sequence location
3R:6,661,427..6,678,590 [-]
Recombination map
3-48
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
GO Summary Ribbons
Families, Domains and Molecular Function
Protein Family (UniProt, Sequence Similarities)
Belongs to the Antp homeobox family. Labial subfamily. (P10105)
Molecular Function (see GO section for details)
Summaries
Gene Group Membership
ANTENNAPEDIA COMPLEX -
The Antennapedia complex (ANT-C) is one of two Hox gene complexes. Hox genes encode homeodomain transcription factors. ANT-C controls the identity of segments that contribute to the head and the anterior thorax. ANT-C homeotic genes show colinearity in their expression patterns with the exception of pb. (Adapted from FBrf0190304).
HOX-LIKE HOMEOBOX TRANSCRIPTION FACTORS -
HOX-like (HOXL) homeobox transcription factors are sequence-specific DNA binding proteins that regulate transcription. They encompass transcription factors encoded by the Hox genes of the Antennapedia and the Bithorax gene complexes and genes closely related in sequence. HOXL transcription factors are major regulators of animal development. (Adapted from FBrf0232555).
UniProt Contributed Function Data
Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Required for proper head development.
(UniProt, P10105)
Phenotypic Description from the Red Book (Lindsley and Zimm 1992)
lab: labial
Null mutations act as recessive embryonic lethals. Animals survive to the end of embryogenesis and have normal thoracic, abdominal, and caudal segments. However, the head is abnormal, and shows defects in derivatives of all of the gnathocephalic segments. There is no obvious homeotic transformation in these animals. Analysis of earlier stages shows abnormalities in the process of head involution. X-ray-induced clones of lab- cells demonstrate that lab function is unnecessary for the development of the adult thorax and abdomen. However, clones in the head fail to develop normally and show deletions in the maxilla and eye. Dorsally the posterior head capsule is transformed toward an apparent thoracic identity. A temperature conditional allele has been used to show a temperature critical period between 6 and 14 hours of embryogenesis. This period coincides with an interval in which head involution, a process disrupted by lab-, takes place. Antisera raised to lab protein have shown it to initially accumulated just anterior to the gnathocephalic region of the germ band at the early stages of segmentation. This protein also is found in a row of cells extending above the gnathal region in the procephalic lobe and more dorsally into the dorsal ridge. As segmentation, germ-band shortening and head involution proceed, the cells expressing the protein are involved in the process complexities of head involution. Finally at the end of morphogenesis, lab positive cells are found in the lateral aspects of the pharynx, the tritocerebral ganglia of the CNS, and the frontal sac. In addition to this expression in the head, lab protein is also found in endodermal cells at the posterior of the anterior midgut and the anterior cells of the posterior midgut. The position and movements of the cephalic cells accumulating lab is consistent with the interpretation that this locus is expressed in the intercalary or most anterior of the gnathal segments.
Gene Model and Products
Number of Transcripts
2
Number of Unique Polypeptides
2

Please see the GBrowse view of Dmel\lab or the JBrowse view of Dmel\lab for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model
An alternative transcript described in FBrf0047938 appears not to be encoded by the sequenced strain; a region of sequence polymorphism eliminates the alternative splice acceptor site.
Gene model reviewed during 5.50
Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0081696
2775
629
FBtr0308035
1586
213
Additional Transcript Data and Comments
Reported size (kB)
2.8, 1.2, 0.8 (northern blot)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0081194
67.5
629
7.83
FBpp0300396
22.3
213
10.18
Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments
Reported size (kDa)
629 (aa); 68 (kD predicted)
Comments
dpp, and indirectly Ubx are required for lab expression in the midgut epithelium.
External Data
Crossreferences
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\lab using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Gene Ontology (13 terms)
Molecular Function (4 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
inferred from electronic annotation with InterPro:IPR017970
(assigned by InterPro )
Biological Process (8 terms)
Terms Based on Experimental Evidence (5 terms)
CV Term
Evidence
References
inferred from genetic interaction with FLYBASE:vvl; FB:FBgn0086680
inferred from mutant phenotype
inferred from genetic interaction with FLYBASE:vvl; FB:FBgn0086680
inferred from mutant phenotype
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
Cellular Component (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
inferred from direct assay
Terms Based on Predictions or Assertions (0 terms)
Expression Data
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
central nervous system

Comment: reference states 12-14 hr AEL

embryonic/larval midgut

Comment: reference states 12-14 hr AEL

microinjection
Stage
Tissue/Position (including subcellular localization)
Reference
northern blot
Stage
Tissue/Position (including subcellular localization)
Reference

Comment: reference states 0-24 hr AEL

Comment: reference states 2-24 hr AEL

Comment: reference states >=2-4 hr AEL

Additional Descriptive Data
RNA-seq data show that CAH9 is enriched in the acidic region of the larval midgut.
Ectopic expression of lab transcripts is seen in midline cells and tracheal placodes of the trunk region in tsh mutants and in the posterior and dorsal part of the prothoracic segment in Antp mutants. In tsh Antp double mutants, lab is expressed ectopically in parasegments 4-13 due to the loss of tsh and more extensively in parasegments 4 and 5 due to loss of Antp. lab expression is unchanged in BXC mutants but ectopic lab expression is observed in the dorsal parts of trunk segments in Antp BXC mutant embryos and in Scr Antp BXC mutant embryos. In tsh Antp BXC mutants, there is more extensive ectopic expression in parasegments 4-13 than in tsh* mutations alone and when Scr function is removed as well, an additional patch of expression is observed in parasegment 3.
lab transcripts are first detected at 2-4 hr of embryogenesis. Expression has peaked by 6-8 hr, and remains constant until 16-20 hr of embryogenesis. Levels of lab transcript decline by 20-24 hr of embryogenesis, and remain at lower levels through larval stages. Transcript levels are low in pupae and transcript is undetectable in adults.
lab transcripts are first detected in ~3hr embryos and continue to be expressed through embryogenesis. A second peak of expression occurs in third instar larvae. Transcripts are detected weakly in pupae and not in adults. Transcripts are first detected by in situ hybridization in the cellular blastoderm embryo in ventro-lateral cells between 74-83% egg length and also in the most posterior somatic cells. The anterior region corresponds to the anlagen of the anterior mandibular lobe, the hypopharyngeal lobe and probably lateral parts of the procephalic lobe. During germ band extension, lab transcripts are observed just anterior to the cephalic furrow and in the invaginating posterior midgut. Expression in these regions persists as the germ band retracts.
This major lab transcript is expressed through most stages of embryonic development with highest levels between 3 and 12 hours. Transcript is detected in the central nervous system posterior to the brain and in the epithelial cells of the middle midgut. Weak signal is also detected in the posterior midgut.
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
No Assay Recorded
Stage
Tissue/Position (including subcellular localization)
Reference
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
Expression in procephalic neuroblasts stage 9-11: tritocerebrum - d1-8, v1-5 (all); deuterocerebrum - v2, v4
lab is expressed in a stripe in the midgut and its expression is restricted to the midgut epithelium and is not detectable in the visceral mesoderm. lab expression coextends with Ubx expression in the adhering mesodermal cell layer. In late stage 12, a gradient of nuclear lab protein forms with the highest protein levels at the posterior end of the domain in parasegment 8. lab expression is abolished in the midgut epithelium of Ubx and dpps4 mutants. It extends anteriorly in a Pc mutant and posteriorly in an abd-A mutant showing that Ubx expression is sufficient to induce lab expression in the adhering midgut epithelium. The overall level, the protein gradient and the nuclear localization of lab protein are decreased in wg mutants.
Nuclear localization of lab protein in the endoderm first becomes detectable in late embryonic stage 12.
lab protein accumulates in the outer layer of the endoderm which underlies parasegment 7 of the visceral mesoderm (where dpp protein is expressed). When dpp is ectopically expressed in the anterior visceral mesoderm as a consequence of ectopic Ubx expression, the labial domain is extended anteriorly to include most of the anterior endoderm underlying the visceral mesoderm.
lab protein expression during embryogenesis is restricted to ectodermal and endodermal derivatives. lab protein is first detected at stage 9 of embryogenesis, in the posterior and anterior midgut primordia, and in the ventral and lateral regions of the procephalic lobe. At stage 12, expression is detected in a discrete section of the midgut, and in the dorsal ridge. The cells in the procephalic lobe expressing lab protein begin to differentiate into epidermal and neural populations at stage 12. The complex pattern of lab expression in the head region as it undergoes the complex process of head involution is described. lab expression is also detected in progenitor sensory cells of the clypeolabrum, thoracic segments, and the tail region.
Marker for
Subcellular Localization
CV Term
Evidence
References
inferred from direct assay
Expression Deduced from Reporters
Reporter: P{0.5labHZ}
Stage
Tissue/Position (including subcellular localization)
Reference
Reporter: P{1.2labHZ}
Stage
Tissue/Position (including subcellular localization)
Reference
Reporter: P{3.65lab66a}
Stage
Tissue/Position (including subcellular localization)
Reference
Reporter: P{lab-GAL4.DR}
Stage
Tissue/Position (including subcellular localization)
Reference
Reporter: P{lab-GAL4.H}
Stage
Tissue/Position (including subcellular localization)
Reference
Reporter: P{lab-lacZ.1/546}
Stage
Tissue/Position (including subcellular localization)
Reference
parasegment 7 of visceral mesoderm

Comment: expression overlaps that of endogenous lab

Reporter: P{lab-lacZ.48/95}
Stage
Tissue/Position (including subcellular localization)
Reference
parasegment 7 of visceral mesoderm

Comment: expression is posterior to that of endogenous lab

Reporter: P{p6.0lab66A}
Stage
Tissue/Position (including subcellular localization)
Reference
Reporter: P{PZ}lab01241
Stage
Tissue/Position (including subcellular localization)
Reference
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\lab in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 1-3
  • Stages(s) 4-6
  • Stages(s) 7-8
  • Stages(s) 9-10
  • Stages(s) 11-12
  • Stages(s) 13-16
Alleles, Insertions, Transgenic Constructs and Phenotypes
Classical and Insertion Alleles ( 24 )
For All Classical and Insertion Alleles Show
 
Allele of lab
Class
Mutagen
Associated Insertion
Stocks
Known lesion
    0
    Yes
    Other relevant insertions
    Transgenic Constructs ( 14 )
    For All Alleles Carried on Transgenic Constructs Show
    Transgenic constructs containing/affecting coding region of lab
    Allele of lab
    Mutagen
    Associated Transgenic Construct
    Stocks
    Transgenic constructs containing regulatory region of lab
    characterization construct
    Name
    Expression Data
    GAL4 construct
    Name
    Expression Data
    Deletions and Duplications ( 28 )
    Summary of Phenotypes
    For more details about a specific phenotype click on the relevant allele symbol.
    Lethality
    Allele
    Sterility
    Allele
    Other Phenotypes
    Allele
    Phenotype manifest in
    Allele
    Orthologs
    Human Orthologs (via DIOPT v7.1)
    Homo sapiens (Human) (31)
    Species\Gene Symbol
    Score
    Best Score
    Best Reverse Score
    Alignment
    Complementation?
    Transgene?
    6 of 15
    Yes
    Yes
    5 of 15
    No
    Yes
    4 of 15
    No
    Yes
    2 of 15
    No
    No
     
    2 of 15
    No
    No
     
    2 of 15
    No
    No
    1 of 15
    No
    No
    1 of 15
    No
    No
    1 of 15
    No
    No
    1 of 15
    No
    No
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    No
    No
     
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    No
    No
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    No
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    No
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    No
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    No
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    No
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    No
    No
    1 of 15
    No
    No
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    No
    No
     
    1 of 15
    No
    No
    1 of 15
    No
    No
     
    1 of 15
    No
    No
    1 of 15
    No
    No
    1 of 15
    No
    No
    1 of 15
    No
    No
    1 of 15
    No
    No
    Model Organism Orthologs (via DIOPT v7.1)
    Mus musculus (laboratory mouse) (31)
    Species\Gene Symbol
    Score
    Best Score
    Best Reverse Score
    Alignment
    Complementation?
    Transgene?
    5 of 15
    Yes
    Yes
    5 of 15
    Yes
    Yes
    4 of 15
    No
    Yes
     
    2 of 15
    No
    No
     
    2 of 15
    No
    No
    2 of 15
    No
    No
    1 of 15
    No
    No
    1 of 15
    No
    No
    1 of 15
    No
    No
    1 of 15
    No
    No
    1 of 15
    No
    No
     
    1 of 15
    No
    No
    1 of 15
    No
    No
    1 of 15
    No
    No
    1 of 15
    No
    No
     
    1 of 15
    No
    No
    1 of 15
    No
    No
     
    1 of 15
    No
    No
     
    1 of 15
    No
    No
     
    1 of 15
    No
    No
    1 of 15
    No
    No
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    No
    No
    1 of 15
    No
    No
    1 of 15
    No
    No
    1 of 15
    No
    No
    1 of 15
    No
    No
    1 of 15
    No
    No
    1 of 15
    No
    No
    1 of 15
    No
    No
    1 of 15
    No
    No
    1 of 15
    No
    No
    Rattus norvegicus (Norway rat) (35)
    5 of 13
    Yes
    Yes
    4 of 13
    No
    Yes
    4 of 13
    No
    Yes
    2 of 13
    No
    No
    2 of 13
    No
    No
    1 of 13
    No
    No
    1 of 13
    No
    No
    1 of 13
    No
    No
    1 of 13
    No
    No
    1 of 13
    No
    No
    1 of 13
    No
    No
    1 of 13
    No
    No
    1 of 13
    No
    No
    1 of 13
    No
    No
    1 of 13
    No
    No
    1 of 13
    No
    No
    1 of 13
    No
    No
    1 of 13
    No
    No
    1 of 13
    No
    No
    1 of 13
    No
    No
    1 of 13
    No
    No
    1 of 13
    No
    No
    1 of 13
    No
    No
    1 of 13
    No
    No
    1 of 13
    No
    No
    1 of 13
    No
    No
    1 of 13
    No
    No
    1 of 13
    No
    No
    1 of 13
    No
    No
    1 of 13
    No
    No
    1 of 13
    No
    No
    1 of 13
    No
    No
    1 of 13
    No
    No
    1 of 13
    No
    No
    Xenopus tropicalis (Western clawed frog) (26)
    4 of 12
    Yes
    Yes
    4 of 12
    Yes
    Yes
    3 of 12
    No
    Yes
    2 of 12
    No
    No
    2 of 12
    No
    No
    1 of 12
    No
    No
    1 of 12
    No
    No
    1 of 12
    No
    No
    1 of 12
    No
    No
    1 of 12
    No
    No
    1 of 12
    No
    No
    1 of 12
    No
    No
    1 of 12
    No
    No
    1 of 12
    No
    No
    1 of 12
    No
    No
    1 of 12
    No
    No
    1 of 12
    No
    No
    1 of 12
    No
    No
    1 of 12
    No
    No
    1 of 12
    No
    No
    1 of 12
    No
    No
    1 of 12
    No
    No
    1 of 12
    No
    No
    1 of 12
    No
    No
    1 of 12
    No
    No
    1 of 12
    No
    No
    Danio rerio (Zebrafish) (36)
    6 of 15
    Yes
    Yes
    5 of 15
    No
    Yes
    4 of 15
    No
    Yes
    3 of 15
    No
    Yes
    2 of 15
    No
    No
    2 of 15
    No
    No
    2 of 15
    No
    No
    2 of 15
    No
    No
    1 of 15
    No
    No
    1 of 15
    No
    No
    1 of 15
    No
    No
     
    1 of 15
    No
    No
    1 of 15
    No
    No
    1 of 15
    No
    No
    1 of 15
    No
    No
    1 of 15
    No
    No
    1 of 15
    No
    No
    1 of 15
    No
    No
    1 of 15
    No
    No
    1 of 15
    No
    No
    1 of 15
    No
    No
    1 of 15
    No
    No
    1 of 15
    No
    No
    1 of 15
    No
    No
    1 of 15
    No
    No
    1 of 15
    No
    No
    1 of 15
    No
    No
    1 of 15
    No
    No
    1 of 15
    No
    No
    1 of 15
    No
    No
    1 of 15
    No
    No
    1 of 15
    No
    No
    1 of 15
    No
    No
    1 of 15
    No
    No
    1 of 15
    No
    No
    1 of 15
    No
    No
    Caenorhabditis elegans (Nematode, roundworm) (9)
    6 of 15
    Yes
    Yes
     
    1 of 15
    No
    No
    1 of 15
    No
    No
    1 of 15
    No
    No
    1 of 15
    No
    No
    1 of 15
    No
    No
    1 of 15
    No
    No
    1 of 15
    No
    No
    1 of 15
    No
    No
    Arabidopsis thaliana (thale-cress) (0)
    No orthologs reported.
    Saccharomyces cerevisiae (Brewer's yeast) (2)
    1 of 15
    Yes
    Yes
    1 of 15
    Yes
    No
    Schizosaccharomyces pombe (Fission yeast) (0)
    No orthologs reported.
    Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG09190C4F )
    Organism
    Common Name
    Gene
    AAA Syntenic Ortholog
    Multiple Dmel Genes in this Orthologous Group
    Drosophila melanogaster
    fruit fly
    Drosophila suzukii
    Spotted wing Drosophila
    Drosophila suzukii
    Spotted wing Drosophila
    Drosophila simulans
    Drosophila sechellia
    Drosophila erecta
    Drosophila yakuba
    Drosophila ananassae
    Drosophila pseudoobscura pseudoobscura
    Drosophila persimilis
    Drosophila willistoni
    Drosophila virilis
    Drosophila mojavensis
    Drosophila grimshawi
    Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150BP2 )
    Organism
    Common Name
    Gene
    Multiple Dmel Genes in this Orthologous Group
    Musca domestica
    House fly
    Musca domestica
    House fly
    Glossina morsitans
    Tsetse fly
    Lucilia cuprina
    Australian sheep blowfly
    Mayetiola destructor
    Hessian fly
    Aedes aegypti
    Yellow fever mosquito
    Anopheles gambiae
    Malaria mosquito
    Culex quinquefasciatus
    Southern house mosquito
    Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( None identified )
    No non-Dipteran orthologies identified
    Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( None identified )
    No non-Insect Arthropod orthologies identified
    Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( None identified )
    No non-Arthropod Metazoa orthologies identified
    Human Disease Model Data
    FlyBase Human Disease Model Reports
      Alleles Reported to Model Human Disease (Disease Ontology)
      Download
      Models ( 0 )
      Allele
      Disease
      Evidence
      References
      Interactions ( 0 )
      Allele
      Disease
      Interaction
      References
      Comments ( 0 )
       
      Human Orthologs (via DIOPT v7.1)
      Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
      Functional Complementation Data
      Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
      Interactions
      Summary of Physical Interactions
      esyN Network Diagram
      Show neighbor-neighbor interactions:
      Select Layout:
      Legend:
      Protein
      RNA
      Selected Interactor(s)
      Interactions Browser

      Please look at the Interaction Group reports for full details of the physical interactions
      protein-protein
      Interacting group
      Assay
      References
      Summary of Genetic Interactions
      esyN Network Diagram
      esyN Network Key:
      Suppression
      Enhancement

      Please look at the allele data for full details of the genetic interactions
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      External Data
      Linkouts
      BioGRID - A database of protein and genetic interactions.
      DroID - A comprehensive database of gene and protein interactions.
      InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
      Pathways
      Gene Group - Pathway Membership (FlyBase)
      External Data
      Linkouts
      Genomic Location and Detailed Mapping Data
      Chromosome (arm)
      3R
      Recombination map
      3-48
      Cytogenetic map
      Sequence location
      3R:6,661,427..6,678,590 [-]
      FlyBase Computed Cytological Location
      Cytogenetic map
      Evidence for location
      84A1-84A1
      Limits computationally determined from genome sequence between P{lacW}casj1C2 and P{PZ}lab01241
      Experimentally Determined Cytological Location
      Cytogenetic map
      Notes
      References
      84A1-84A2
      (determined by in situ hybridisation)
      84A-84A
      (determined by in situ hybridisation)
      Experimentally Determined Recombination Data
      Location
      Left of (cM)
      Right of (cM)
      Notes
      Stocks and Reagents
      Stocks (14)
      Genomic Clones (27)
      cDNA Clones (14)
       

      Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

      cDNA clones, fully sequences
      BDGP DGC clones
      Other clones
        Drosophila Genomics Resource Center cDNA clones

        For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

        cDNA Clones, End Sequenced (ESTs)
        BDGP DGC clones
          RNAi and Array Information
          Linkouts
          DRSC - Results frm RNAi screens
          GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
          Antibody Information
          Laboratory Generated Antibodies
          Commercially Available Antibodies
           
          Other Information
          Relationship to Other Genes
          Source for database identify of
          Source for identity of: lab CG1264
          Source for database merge of
          Additional comments
          Other Comments
          lab is required for neuronal differentiation in the developing embryonic brain.
          Endoderm expression from a lab enhancer depends on multiple CREs, cAMP response elements from a Ubx enhancer. CrebB-17A binds to the Ubx cAMP response element (CRE), located in an enhancer, and ubiquitous expression of a dominant negative form of CrebB-17A reduces lab expression in the endoderm.
          Effects of overexpression of ANTP-C genes on tarsal segmentation in ss mutants is studied.
          dpp, by inducing kay, broadly defines an endodermal region which thus becomes predisposed to express lab. kay cooperates with signal-activated response factors to confer the precise pattern of lab expression in the endoderm.
          One of a class of genes with TATA-less promoters that have a subset of the conserved DPE sequence.
          Protein-DNA interactions are important for forming the lab-exd-DNA complex. Data suggests a model in which lab and exd bind DNA as a heterodimer in a head-to-tail orientation.
          A 20bp oligonucleotide from the 5' region of Mmus\Hoxb1, a homolog of lab, is sufficient to direct an expression pattern in Drosophila very similar to endogenous lab. This expression requires lab abd exd function. In vitro DNA binding studies reveal that lab requires exd to bind DNA with high affinity.
          Rescue of lab null mutants by the chicken ortholog Hoxb-1 demonstrates that the function of Hox genes is phylogenetically conserved.
          A phylogenetic analysis of the Antp-class of homeodomains in nematode, Drosophila, amphioxus, mouse and human indicates that the 13 cognate group genes of this family can be divided into two major groups. Genes that are phylogenetically close are also closely located on the chromosome, suggesting that the colinearity between gene expression and gene arrangement was generated by successive tandem gene duplications and that the gene arrangement has been maintained by some sort of selection.
          Virtually all of the P{r4/lacZ} expression in Drosophila depends on the same three conserved sequence elements involved in regulating expression in the mouse and lab expression. Expression in the head and the visceral mesoderm requires exd function.
          Expression of lab in the endoderm coincides with the copper cells and is required for their formation and maintenance.
          Ectopic expression of dpp eliminates Scr and Antp expression, attenuating abd-A expression, inducing Ubx, dpp, wg and tsh expression in the visceral mesoderm and inducing lab expression in the apposing endoderm. The result is failure of all of the morphogenetic events except formation of midgut constriction 2.
          One of the homeodomain loci identified in a screen for genes encoding DNA binding proteins capable of binding to a consensus Engrailed binding site.
          The homologs of Antp, ftz, Scr, Dfd, Ama, bcd, zen, pb and lab, but not zen2 are all present in D.pseudoobscura.pseudoobscura, in the same linear order and similarly spaced along the chromosome as in D.melanogaster.
          Comparative analysis of the homeobox sequences reveals the subdivision of the Antp-type homeobox genes into three classes early in metazoan evolution, one includes Abd-B, the second includes abd-A, Ubx, Antp, Scr, Dfd and ftz, and the third includes zen, zen2, pb and lab.
          The effect of ectopic expression of lab was investigated on the normal development of sensory organs in the embryonic PNS.
          lab gene product is not required for salivary gland development, at least up until the cuticle forms.
          ae expression is not modulated by lab. lab is expressed ectopically in embryos deficient for ae and Antp.
          Dissection of 5' sequences of labial gene reveal 2 types of cis-acting response elements: one mediates labial-dependent activity, providing evidence that labial induction in the endoderm is autoregulatory, the other responds to the dpp gene product.
          Cis-acting regulatory elements necessary for the proper spatial and temporal expression of lab have been determined.
          The roles of Dfd and lab have been studied through an analysis of their expression patterns in embryonic and imaginal tissues of mutant individuals.
          Ubx, abd-A, dpp, wg and lab have interacting gene products involved in the induction process between the visceral mesoderm and the gut epithelium in the embryo. lab antibody staining demonstrated that lab expression is restricted to midgut epithelial cells but coextends with Ubx expression in the adhering mesodermal cell layer. dpp and Ubx are required for lab expression in the midgut. lab staining is not altered in wg mutants.
          lab- flies are not viable and die at the embryo-first larval instar boundary.
          Mesodermal expression of dpp is required for lab expression in the midgut endoderm suggesting that dpp migrates from the mesoderm to endoderm to induce lab expression.
          A consequence of dpp expression in the visceral mesoderm cells of the anterior midgut is the induction of lab in the underlying endoderm cells.
          The correlation of lab mutant defects and lab gene expression was determined to increase understanding of Drosophila head segmental organization.
          A developmental genetic analysis of lab has been carried out.
          The molecular structure and spatial expression of the lab gene has been examined.
          The DNA sequences of the homeobox region of 11 Drosophila genes, including lab, have been compared.
          Null mutations act as recessive embryonic lethals. Animals survive to the end of embryogenesis and have normal thoracic, abdominal and caudal segments. However, the head is abnormal and shows defects in derivatives of all of the gnathocephalic segments. There is no obvious homeotic transformation in these animals. Analysis of earlier stages shows abnormalities in the process of head involution. X-ray-induced clones of lab- cells demonstrate that lab function is unnecessary for the development of the adult thorax and abdomen. However, clones in the head fail to develop normally and show deletions in the maxilla and eye. Dorsally the posterior head capsule is transformed toward an apparent thoracic identity. A temperature conditional allele has been used to show a temperature critical period between 6 and 14 hours of embryogenesis. This period coincides with an interval in which head involution, a process disrupted by lab-, takes place. Antisera raised to lab protein have shown it to initially accumulated just anterior to the gnathocephalic region of the germ band at the early stages of segmentation. This protein also is found in a row of cells extending above the gnathal region in the procephalic lobe and more dorsally into the dorsal ridge. As segmentation, germ-band shortening and head involution proceed, the cells expressing the protein are involved in the process complexities of head involution. Finally at the end of morphogenesis, lab positive cells are found in the lateral aspects of the pharynx, the tritocerebral ganglia of the CNS and the frontal sac. In addition to this expression in the head, lab protein is also found in endodermal cells at the posterior of the anterior midgut and the anterior cells of the posterior midgut. The position and movements of the cephalic cells accumulating lab is consistent with the interpretation that this locus is expressed in the intercalary or most anterior of the gnathal segments.
          Origin and Etymology
          Discoverer
          Etymology
          Identification
          External Crossreferences and Linkouts ( 48 )
          Crossreferences
          NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
          GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
          GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
          RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
          UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
          UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
          Other crossreferences
          BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
          Linkouts
          BioGRID - A database of protein and genetic interactions.
          Drosophila Genomics Resource Center - Drosophila Genomics Resource Center cDNA clones
          DroID - A comprehensive database of gene and protein interactions.
          DRSC - Results frm RNAi screens
          Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
          FLIGHT - Cell culture data for RNAi and other high-throughput technologies
          FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
          Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
          Flygut - An atlas of the Drosophila adult midgut
          FlyMine - An integrated database for Drosophila genomics
          GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
          iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
          Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
          InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
          KEGG Genes - Molecular building blocks of life in the genomic space.
          modMine - A data warehouse for the modENCODE project
          Synonyms and Secondary IDs (18)
          Reported As
          Symbol Synonym
          BG:DS00004.9
          F90
          l(3)01241
          l(3)84Ac
          Secondary FlyBase IDs
          • FBgn0010742
          Datasets (0)
          Study focus (0)
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          References (393)