FB2022_03, released June 9, 2022

Gene: Dmel\Lam

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General Information
Symbol
Dmel\Lam
Species
D. melanogaster
Name
Lamin
Annotation Symbol
CG6944
Feature Type
protein_coding_gene
FlyBase ID
FBgn0002525
Gene Model Status
Current
Stock Availability
21 publicly available
Gene Summary
Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin (PubMed:3126192, PubMed:15035436). May have a role in the localization of the LEM domain proteins Ote, bocks and MAN1 to the nuclear membrane (PubMed:15035436, PubMed:16439308). In spermatocytes, plays a role in maintaining type-A lamin LamC nuclear localization; regulates meiotic cytokinesis by maintaining the structure of the spindle envelope, and by contributing to the formation of the contractile ring and central spindle (PubMed:27402967). Required for nuclear migration and to link the microtubule organizing center (MTOC) to the nucleus (PubMed:14617811). In addition, is required for nuclear envelope localization of klar (PubMed:14617811). (UniProt, P08928)
Contribute a Gene Snapshot for this gene.
All Summaries
Also Known As

lamin Dm0, Dm0, lamin Dm0, Dm0, lamin B

Key Links
Genomic Location
Cytogenetic map
25E6-25E6
Sequence location
Recombination map
2-17
RefSeq locus
NT_033779 REGION:5542480..5546642
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (35 terms)
Molecular Function (4 terms)
Terms Based on Experimental Evidence (4 terms)
CV Term
Evidence
References
enables chromatin binding
inferred from direct assay
(Verboon et al., 2015)
enables protein binding
inferred from physical interaction with FLYBASE:fs(1)Ya; FB:FBgn0000927
(Goldberg et al., 1998)
inferred from physical interaction with FLYBASE:Ote; FB:FBgn0266420
(Goldberg et al., 1998)
inferred from physical interaction with FLYBASE:wash; FB:FBgn0033692
(Verboon et al., 2015)
enables signaling receptor binding
inferred from physical interaction with UniProtKB:Q8MLV1
(assigned by UniProt )
(Wagner et al., 2004)
enables structural constituent of cytoskeleton
inferred from mutant phenotype
(Lenz-Bohme et al., 1997)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
enables structural constituent of cytoskeleton
inferred from biological aspect of ancestor with PANTHER:PTN001145669
(assigned by GO_Central )
(Gaudet et al., 2011)
Biological Process (25 terms)
Terms Based on Experimental Evidence (25 terms)
CV Term
Evidence
References
involved_in central nervous system development
inferred from mutant phenotype
(Osouda et al., 2005)
involved_in chitin-based cuticle development
inferred from mutant phenotype
(Osouda et al., 2005)
involved_in compound eye morphogenesis
inferred from mutant phenotype
(Osouda et al., 2005)
involved_in digestive tract morphogenesis
inferred from mutant phenotype
(Osouda et al., 2005)
involved_in gonad morphogenesis
inferred from mutant phenotype
(Osouda et al., 2005)
involved_in heterochromatin assembly
inferred from mutant phenotype
(Verboon et al., 2015, Shevelyov et al., 2009)
inferred from direct assay
(Dialynas et al., 2010)
involved_in heterochromatin organization
inferred from mutant phenotype
(Chen et al., 2014)
involved_in male meiosis cytokinesis
inferred from mutant phenotype
(assigned by UniProt )
(Hayashi et al., 2016)
involved_in meiosis I cytokinesis
inferred from mutant phenotype
(assigned by UniProt )
(Hayashi et al., 2016)
involved_in mitotic nuclear membrane reassembly
inferred from direct assay
(Ulitzur et al., 1992)
acts_upstream_of negative regulation of cell population proliferation
inferred from mutant phenotype
(Chen et al., 2014)
acts_upstream_of negative regulation of immune response
inferred from mutant phenotype
(Chen et al., 2014)
involved_in nuclear envelope organization
inferred from mutant phenotype
(Osouda et al., 2005, Lenz-Bohme et al., 1997)
involved_in nuclear membrane organization
inferred from direct assay
(Prufert et al., 2004)
involved_in nuclear migration
inferred from mutant phenotype
(Patterson et al., 2004)
involved_in nuclear pore localization
inferred from mutant phenotype
(Lenz-Bohme et al., 1997)
involved_in nucleus organization
inferred from mutant phenotype
(assigned by UniProt )
(Wagner et al., 2004)
involved_in positive regulation of actin filament polymerization
inferred from mutant phenotype
(assigned by UniProt )
(Dopie et al., 2015)
involved_in positive regulation of cytokinesis, actomyosin contractile ring assembly
inferred from mutant phenotype
(assigned by UniProt )
(Hayashi et al., 2016)
involved_in positive regulation of protein localization to nucleus
inferred from mutant phenotype
(assigned by UniProt )
(Hayashi et al., 2016)
involved_in positive regulation of spindle assembly
inferred from mutant phenotype
(assigned by UniProt )
(Hayashi et al., 2016)
involved_in protein localization to nuclear envelope
inferred from mutant phenotype
(Wagner et al., 2004)
involved_in spermatocyte division
inferred from mutant phenotype
(assigned by UniProt )
(Hayashi et al., 2016)
involved_in spermatogenesis
inferred from mutant phenotype
(Chen et al., 2013)
involved_in terminal branching, open tracheal system
inferred from mutant phenotype
(Guillemin et al., 2001)
Terms Based on Predictions or Assertions (5 terms)
CV Term
Evidence
References
involved_in heterochromatin assembly
inferred from biological aspect of ancestor with PANTHER:PTN001145669
(assigned by GO_Central )
(Gaudet et al., 2011)
involved_in nuclear envelope organization
inferred from biological aspect of ancestor with PANTHER:PTN001145669
(assigned by GO_Central )
(Gaudet et al., 2011)
involved_in nuclear migration
inferred from biological aspect of ancestor with PANTHER:PTN001145669
(assigned by GO_Central )
(Gaudet et al., 2011)
involved_in nuclear pore localization
inferred from biological aspect of ancestor with PANTHER:PTN001145669
(assigned by GO_Central )
(Gaudet et al., 2011)
involved_in protein localization to nuclear envelope
inferred from biological aspect of ancestor with PANTHER:PTN001145669
(assigned by GO_Central )
(Gaudet et al., 2011)
Cellular Component (7 terms)
Terms Based on Experimental Evidence (6 terms)
CV Term
Evidence
References
located_in lamin filament
inferred from direct assay
(Klapper et al., 1997)
located_in nuclear envelope lumen
inferred from direct assay
(assigned by UniProt )
(Harel et al., 1989)
located_in nuclear envelope
inferred from direct assay
(Verboon et al., 2015, Schulze et al., 2009, Pilot et al., 2006, Wagner et al., 2004)
located_in nuclear lamina
inferred from direct assay
(Verboon et al., 2020, Shevelyov et al., 2009, Riemer et al., 1995)
inferred from direct assay
(assigned by UniProt )
(Hayashi et al., 2016)
located_in nucleus
inferred from direct assay
(Furukawa et al., 2003)
colocalizes_with spindle envelope
inferred from direct assay
(assigned by UniProt )
(Hayashi et al., 2016)
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
located_in lamin filament
traceable author statement
(Smith and Fisher, 1989)
is_active_in nuclear envelope
inferred from biological aspect of ancestor with PANTHER:PTN001145669
(assigned by GO_Central )
(Gaudet et al., 2011)
is_active_in nuclear lamina
inferred from biological aspect of ancestor with PANTHER:PTN001145669
(assigned by GO_Central )
(Gaudet et al., 2011)
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the intermediate filament family. (P08928)
Protein Signatures (InterPro)
Summaries
Gene Group (FlyBase)
LAMINS -
Lamins are type V intermediate filament that form the major component of the nuclear lamina (FBrf0200518).
Protein Function (UniProtKB)
Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin (PubMed:3126192, PubMed:15035436). May have a role in the localization of the LEM domain proteins Ote, bocks and MAN1 to the nuclear membrane (PubMed:15035436, PubMed:16439308). In spermatocytes, plays a role in maintaining type-A lamin LamC nuclear localization; regulates meiotic cytokinesis by maintaining the structure of the spindle envelope, and by contributing to the formation of the contractile ring and central spindle (PubMed:27402967). Required for nuclear migration and to link the microtubule organizing center (MTOC) to the nucleus (PubMed:14617811). In addition, is required for nuclear envelope localization of klar (PubMed:14617811).
(UniProt, P08928)
Phenotypic Description (Red Book; Lindsley and Zimm 1992)
Lam: Lamin
The structural gene for nuclear lamin. Translated as a prolamin of apparent molecular mass of 76-kd, which is quickly processed to a 74-kd species, presumably by proteolysis. The processed polypeptide is assembled into the nuclear envelope, where it becomes phosphorylated and again attains a mass of 76 kd (Smith, Gruenbaum, Berrios, and Fisher, 1987, J. Cell Biol. 105: 771-90).
Summary (Interactive Fly)

an intermediate filament protein - chromatin associated protein - Lamin binds to scaffold/matrix-associated regions, DNA sequences that are held responsiblefor mediating the interaction between the nuclear matrix and chromatin.

Gene Model and Products
Number of Transcripts
4
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\Lam for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure New Section
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry P08928)

If you don't see the viewer to the right, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Comments on Gene Model

Gene model reviewed during 5.55

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Gene model reviewed during 5.45

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.56

Sequence Ontology: Class of Gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Lam-RA
FBtr0079100
NM_057268
3129
622
Lam-RB
FBtr0307058
NM_001258963
3235
622
Lam-RC
FBtr0307059
NM_001258964
3504
622
Lam-RD
FBtr0346460
NM_001298700
3191
622
Additional Transcript Data and Comments
Reported size (kB)

3.0, 2.8 (northern blot)

(Gruenbaum et al., 1988)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
Lam-PA
FBpp0078733
71.3
622
6.40
P08928
NP_476616
AAF52262
Lam-PB
FBpp0297901
71.3
622
6.40
P08928
NP_001245892
AFH03566
Lam-PC
FBpp0297902
71.3
622
6.40
P08928
NP_001245893
AFH03567
Lam-PD
FBpp0312110
71.3
622
6.40
P08928
NP_001285629
AHN54144
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

622 aa isoforms: Lam-PA, Lam-PB, Lam-PC, Lam-PD
Additional Polypeptide Data and Comments
Reported size (kDa)

622 (aa); 76 (kD observed); 71 (kD predicted)

(Gruenbaum et al., 1988)
Comments
External Data
Subunit Structure (UniProtKB)

Interacts directly with LBR (PubMed:15054108). Interacts with MAN1 (PubMed:16439308). Interacts with Ote (PubMed:9632815, PubMed:22751930).

(UniProt, P08928)
Post Translational Modification

Three forms of lamin have been identified in D.melanogaster, lamin Dm0 is rapidly processed to lamin Dm1 in the cytoplasm, Dm1 is then assembled in the nuclear envelope and is then phosphorylated, forming lamin Dm2.

(UniProt, P08928)
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Lam using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that the Fly Cell Atlas project found the gene expressed in that cell type. Darker colors mean that more cells of that cell type express the gene:
 low
high 
Colorless tiles indicate that there is no scRNAseq data for the gene in that cell type.
Colored tiles in ribbon indicate that expression data (RNA and/or protein) has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
Colored tiles in the ribbon indicate the average RNA expression level of the gene at the indicated stages:
 low
high 
as determined by RNA-seq (RPKM) using whole organism samples modENCODE, Brown et al., 2014. For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 1 -- 3
organism

Comment: maternally deposited

(Fisher et al., 2012)
embryonic stage 4 -- 6
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 7 -- 8
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 9 -- 10
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 11 -- 12
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 13 -- 16
organism | ubiquitous
(Fisher et al., 2012)
northern blot
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage
(Riemer et al., 1995)
embryonic stage -- adult stage
(Riemer et al., 1995, Gruenbaum et al., 1988)
Additional Descriptive Data

The 3.0 kb Lam transcript is expressed at all stages, from embryo to adult. Although the 2.8 kb Lam transcript predominates in late oogenesis and early embryogenesis, the 3.0 kb transcript predominates starting at 2-3 hours of embryogenesis. Both transcripts encode the same polypeptide, and differ in their 3'-untranslated regions.

(Gruenbaum et al., 1988)
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage -- adult stage
(Ashery-Padan et al., 1997)
embryonic stage 12 -- 13
organism
(Harel et al., 1989)
embryonic stage 14
Malpighian tubule Type I cell
(Campbell et al., 2010)
Malpighian tubule Type II cell
(Campbell et al., 2010)
prepupal stage P4 -- pupal stage P5
salivary gland
• nucleus

Comment: 8-14 hr APF; full-length 76 kD Lamin

(Martin and Baehrecke, 2004)
pupal stage P5
salivary gland

Comment: 12-14 hr APF; cleaved 45 kD Lamin

(Martin and Baehrecke, 2004)
spermatogenesis & adult stage | male
spermatid cyst
(Arama et al., 2003)
adult stage | male & spermatogenesis
cyst progenitor cell
(Chen et al., 2013)
cyst cell of testis
(Chen et al., 2013)
oogenesis
(Ashery-Padan et al., 1997)
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Aradska et al., 2015)
day 7 of adulthood -- day 49 of adulthood
adult heart
(Cammarato et al., 2011)
western blot
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage
(Riemer et al., 1995)
embryonic stage -- adult stage
(Riemer et al., 1995)
larval stage
(Riemer et al., 1995)
prepupal stage P4 -- pupal stage P5
salivary gland

Comment: 8-14 hr APF; full-length 76 kD Lamin

(Martin and Baehrecke, 2004)
pupal stage P5
salivary gland

Comment: 12-14 hr APF; cleaved 45 kD Lamin

(Martin and Baehrecke, 2004)
Additional Descriptive Data

Lam protein is a nuclear envelope polypeptide which remains in an envelope-like structure enclosing the mitotic apparatus throughout mitosis.

(Campbell et al., 2010, Harel et al., 1989)

Full-length Lam is abundant at 8 hours APF, but decreases from 10-12 hours APF, and is at low to absent levels at 14 hours APF. Cleaved Lam is observed starting at 10 hours APF, and is abundant by 14 hours APF.

(Martin and Baehrecke, 2004)

Lam protein is a component of the individualization complex in late spermatogenesis.

(Arama et al., 2003)

Between stages S4 and S6-S7 of oogenesis, Lam protein localizes to the nuclear periphery in all cell types, but after stage S6-S7, nucleoplasmic and cytoplasmic accumulation is also observed. During larval, pupal and adult stages Lam protein localizes to the nucleus of most cells. No Lam protein is detected during stages 6-11 of spermiogenesis.

(Ashery-Padan et al., 1997, Riemer et al., 1995)

Lam protein is nuclear. There is some, but not complete, overlap in subcellular Lam and LamC protein expression.

(Riemer et al., 1995, Harel et al., 1989)
Marker for
 
nuclear membrane
(Minakhina et al., 2014)
Subcellular Localization
CV Term
Evidence
References
located_in lamin filament
inferred from direct assay
(Klapper et al., 1997)
located_in nuclear envelope lumen
inferred from direct assay
(assigned by UniProt )
(Harel et al., 1989)
located_in nuclear envelope
inferred from direct assay
(Verboon et al., 2015, Schulze et al., 2009, Pilot et al., 2006, Wagner et al., 2004)
located_in nuclear lamina
inferred from direct assay
(Verboon et al., 2020, Shevelyov et al., 2009, Riemer et al., 1995)
inferred from direct assay
(assigned by UniProt )
(Hayashi et al., 2016)
located_in nucleus
inferred from direct assay
(Furukawa et al., 2003)
colocalizes_with spindle envelope
inferred from direct assay
(assigned by UniProt )
(Hayashi et al., 2016)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Lam in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 4-6
  • Stages(s) 7-8
  • Stages(s) 11-12
  • Stages(s) 13-16
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 25 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 25 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Lam
Transgenic constructs containing regulatory region of Lam
Aberrations (Deficiencies and Duplications) ( 26 )
Inferred from experimentation ( 26 )
Gene disrupted in
Df(2L)BSC169
(Christensen and Cook, 2006.7.11)
Df(2L)cl1
(BDGP Project Members, 1994-1999, Szidonya and Reuter, 1988, Szidonya and Reuter, 1988, Kotarski et al., 1983)
Df(2L)cl2
(Szidonya and Reuter, 1988, Szidonya and Reuter, 1988, Kotarski et al., 1983)
Df(2L)cl7
(BDGP Project Members, 1994-1999, Szidonya and Reuter, 1988, Szidonya and Reuter, 1988, Kotarski et al., 1983)
Df(2L)cl-h1
(Eberl et al., 1997, Szidonya and Reuter, 1988, Szidonya and Reuter, 1988)
Df(2L)cl-h2
(Eberl et al., 1997, Szidonya and Reuter, 1988, Szidonya and Reuter, 1988)
Df(2L)cl-h3
(Guillemin et al., 2001, Szidonya and Reuter, 1988, Szidonya and Reuter, 1988)
Df(2L)cl-h4
(Patterson et al., 2004, Eberl et al., 1997, Szidonya and Reuter, 1988, Szidonya and Reuter, 1988)
Df(2L)Exel6012
(Ryder, 2004.4.26)
Df(2L)Exel7023
(Ryder, 2004.4.26)
Df(2L)Gpdh1-A
(Osouda et al., 2005, Guillemin et al., 2001, Spradling et al., 1999, Guillemin, 1997.6.19, BDGP Project Members, 1994-1999, Kotarski et al., 1983)
Df(2L)PM101
(BDGP Project Members, 1994-1999)
Df(2L)sc19-4
(Szidonya and Reuter, 1988, Szidonya and Reuter, 1988)
Df(2L)sc19-12
(Szidonya and Reuter, 1988, Szidonya and Reuter, 1988)
Ts(YSt;2Lt)B236+Ts(YSt;2Rt)H164
(Kotarski et al., 1983)
Gene not disrupted in
Df(2L)2802
(Kotarski et al., 1983)
Df(2L)E110
(Spradling et al., 1999, BDGP Project Members, 1994-1999)
Df(2L)Gpdh1-75
(Kotarski et al., 1983)
Df(2L)Gpdh1-78
(Kotarski et al., 1983)
Df(2L)sc19-1
(Szidonya and Reuter, 1988, Szidonya and Reuter, 1988)
Df(2L)sc19-5
(Szidonya and Reuter, 1988, Szidonya and Reuter, 1988)
Df(2L)sc19-6
(Szidonya and Reuter, 1988, Szidonya and Reuter, 1988)
Df(2L)sc19-7
(Szidonya and Reuter, 1988, Szidonya and Reuter, 1988)
Df(2L)sc19-8
(Szidonya and Reuter, 1988, Szidonya and Reuter, 1988)
Df(2L)tkv2
(Spradling et al., 1999, BDGP Project Members, 1994-1999, Szidonya and Reuter, 1988, Szidonya and Reuter, 1988)
Df(2L)tkv3
(BDGP Project Members, 1994-1999, Szidonya and Reuter, 1988, Szidonya and Reuter, 1988)
Inferred from location ( 0 )
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal
lethal, with Scer\GAL4C23
lethal, with Scer\GAL4da.G32
lethal, with Scer\GAL4elav.PU
lethal, with Scer\GAL4how-24B
lethal, with Scer\GAL4moody.SPG
lethal, with Scer\GAL4repo.PU
lethal | pharate adult stage
lethal | pharate adult stage (with Df(2L)Gpdh1-A)
lethal | pupal stage
lethal | pupal stage (with Df(2L)Gpdh1-A)
lethal | recessive
lethal | temperature conditional, with Scer\GAL4arm.PS
lethal - all die before end of larval stage
lethal - all die before end of larval stage | recessive
lethal - all die during P-stage, with Scer\GAL4Mef2.PR
partially lethal, with Scer\GAL4GMR.PU
partially lethal | recessive
partially lethal - majority die
partially lethal - majority die (with Lam14)
partially lethal - majority die (with LamP)
partially lethal - majority die | larval stage
partially lethal - majority die | larval stage (with Df(2L)cl-h1)
partially lethal - majority die | pupal stage
partially lethal - majority die | pupal stage (with Df(2L)cl-h1)
partially lethal - majority live (with Df(2L)cl-h4)
partially lethal - majority live | recessive
viable
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4He.PZ
viable, with Scer\GAL4nrv2.PS
viable, with Scer\GAL4pnr-MD237
viable, with Scer\GAL4tin.CΔ4
viable | temperature conditional, with Scer\GAL4arm.PS
Sterility
Allele
female sterile | recessive
fertile
male sterile | recessive
sterile
sterile (with Lam14)
sterile (with LamP)
sterile | female | germline clone | recessive
Other Phenotypes
Allele
abnormal cell growth
abnormal developmental rate
abnormal developmental rate (with Df(2L)Gpdh1-A)
abnormal flight
abnormal locomotor behavior
abnormal locomotor behavior (with Df(2L)Gpdh1-A)
abnormal locomotor behavior (with Lam14)
abnormal locomotor behavior (with LamP)
abnormal locomotor behavior | adult stage, with Scer\GAL4Mhc.Switch.PO
abnormal locomotor behavior | adult stage, with Scer\GAL4Switch1.106
abnormal locomotor behavior | larval stage
abnormal locomotor behavior | recessive
abnormal size | adult stage, with Scer\GAL4ey.PH
abnormal size | oogenesis | oogenesis
abnormal size | pupal stage
enhancer of variegation, with In(1)wm4
flightless | recessive
hyperplasia, with Scer\GAL4Cg.PA
hyperplasia (with LamD395)
hyperplasia (with LamK2)
hyperplasia | pupal stage
increased occurrence of cell division | pupal stage
short lived, with Scer\GAL4Mhc.Switch.PO
short lived, with Scer\GAL4Switch1.106
short lived, with Scer\GAL4Ugt36A1.PK
some die during larval stage
some die during pupal stage
some die during pupal stage (with Lam14)
some die during pupal stage (with LamP)
some die during third instar larval stage | recessive
suppressor of variegation, with Dp(?;2)bwD
suppressor of variegation, with In(2R)bwVDe2
uncoordinated, with Scer\GAL4Act5C.PI
uncoordinated | recessive
visible, with Scer\GAL4GMR.PU
visible, with Scer\GAL4pnr-MD237
visible | adult stage
visible | adult stage, with Scer\GAL4Act5C.PI
visible | adult stage, with Scer\GAL4ey.PH
visible | adult stage, with Scer\GAL4GMR.PF
visible | adult stage (with Df(2L)cl-h4)
wild-type
Phenotype manifest in
Allele
adult brain
adult cuticle | pharate adult stage
adult fat body, with Scer\GAL4Switch1.106
adult midgut, with Scer\GAL4Cg.PA
adult midgut (with LamD395)
adult midgut (with LamK2)
adult midgut | pharate adult stage
adult pericardial cell, with Scer\GAL4Ugt36A1.PK
annulate lamellae
brain, with Scer\GAL4elav.Switch.PO
chaeta, with Scer\GAL4pnr-MD237
cyst cell of testis, with Scer\GAL4NP1624
cyst cell of testis (with Df(2L)cl-h1)
cystocyte & nucleus & pharate adult
cyst progenitor cell, with Scer\GAL4NP1624
cyst progenitor cell (with Df(2L)cl-h1)
dorsal appendage | germline clone
dorsal appendage | germline clone | increased number
dorsal tracheal branch primordium
egg | germline clone
egg chamber | germline clone
egg chamber | pharate adult stage
eye
eye, with Scer\GAL4Act5C.PI
eye, with Scer\GAL4ey.PH
eye, with Scer\GAL4GMR.PF
eye, with Scer\GAL4GMR.PU
eye (with Df(2L)cl-h4)
eye & nucleus & pharate adult
eye disc & nucleus | ectopic
eye disc & nucleus | ectopic (with Df(2L)cl-h4)
eye disc & nucleus | ectopic | somatic clone
gamete | female | germline clone
ganglionic tracheal branch primordium
germline stem cell, with Scer\GAL4NP1624
germline stem cell (with Df(2L)cl-h1)
gnathal ganglion | pharate adult stage
gonad | male & pharate adult
longitudinal muscle cell, with Scer\GAL4Mhc.Switch.PO
longitudinal muscle cell | pharate adult stage
male germline cell (with Df(2L)cl-h1)
nephrocyte | larval stage, with Scer\GAL4Ugt36A1.PK
nuclear membrane
nuclear membrane | adult stage
nuclear membrane | pharate adult stage
nuclear pore
nuclear pore | larval stage, with Scer\GAL4Ugt36A1.PK
nucleus & eye photoreceptor cell & eye disc
nucleus | adult stage, with Scer\GAL4elav.Switch.PO
nucleus | adult stage, with Scer\GAL4Mhc.Switch.PO
nucleus | adult stage, with Scer\GAL4Switch1.106
nurse cell | germline clone
ommatidium
oocyte
oocyte | germline clone
ovariole
ovary
ovary | pharate adult stage
pigment cell | pharate adult stage
presumptive embryonic/larval muscle system
presumptive embryonic/larval tracheal system
rhabdomere
rhabdomere (with Df(2L)cl-h4)
spermatozoon
trichogen cell, with Scer\GAL4pnr-MD237
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (36)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\LMNA
12 of 15
Yes
Yes
6  
Hsap\LMNB2
12 of 15
Yes
Yes
1  
Hsap\LMNB1
11 of 15
No
Yes
1  
Hsap\DES
2 of 15
No
Yes
Hsap\VIM
2 of 15
No
Yes
Hsap\CFAP53
1 of 15
No
No
Hsap\GFAP
1 of 15
No
Yes
9  
Hsap\INA
1 of 15
No
Yes
Hsap\KRT3
1 of 15
No
Yes
Hsap\KRT5
1 of 15
No
Yes
2  
Hsap\KRT6A
1 of 15
No
Yes
Hsap\KRT6B
1 of 15
No
Yes
Hsap\KRT7
1 of 15
No
Yes
Hsap\KRT8
1 of 15
No
Yes
Hsap\KRT9
1 of 15
No
Yes
Hsap\KRT10
1 of 15
No
Yes
Hsap\KRT12
1 of 15
No
Yes
Hsap\KRT13
1 of 15
No
Yes
Hsap\KRT14
1 of 15
No
Yes
3  
Hsap\KRT15
1 of 15
No
Yes
Hsap\KRT16
1 of 15
No
Yes
Hsap\KRT17
1 of 15
No
Yes
Hsap\KRT19
1 of 15
No
Yes
Hsap\KRT31
1 of 15
No
Yes
Hsap\KRT33A
1 of 15
No
Yes
Hsap\KRT33B
1 of 15
No
Yes
Hsap\KRT35
1 of 15
No
Yes
Hsap\KRT75
1 of 15
No
Yes
Hsap\KRT76
1 of 15
No
Yes
Hsap\LMNTD1
1 of 15
No
Yes
1  
Hsap\LMNTD2
1 of 15
No
Yes
Hsap\LOC100653049
1 of 15
No
Yes
Hsap\MIR7108
1 of 15
No
Yes
Hsap\NEFH
1 of 15
No
Yes
Hsap\NEFL
1 of 15
No
Yes
Hsap\NEFM
1 of 15
No
Yes
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (27)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Lmnb2
13 of 15
Yes
Yes
Mmus\Lmna
11 of 15
No
Yes
Mmus\Lmnb1
11 of 15
No
Yes
Mmus\Des
2 of 15
No
Yes
Mmus\Vim
2 of 15
No
Yes
Mmus\Gfap
1 of 15
No
Yes
Mmus\Ina
1 of 15
No
Yes
Mmus\Krt5
1 of 15
No
Yes
Mmus\Krt6a
1 of 15
No
Yes
Mmus\Krt6b
1 of 15
No
Yes
Mmus\Krt8
1 of 15
No
Yes
Mmus\Krt13
1 of 15
No
Yes
Mmus\Krt14
1 of 15
No
Yes
Mmus\Krt15
1 of 15
No
Yes
Mmus\Krt16
1 of 15
No
Yes
Mmus\Krt17
1 of 15
No
Yes
Mmus\Krt19
1 of 15
No
Yes
Mmus\Krt35
1 of 15
No
Yes
Mmus\Krt36
1 of 15
No
Yes
Mmus\Krt42
1 of 15
No
Yes
Mmus\Krt75
1 of 15
No
Yes
Mmus\Krt79
1 of 15
No
Yes
Mmus\Krt90
1 of 15
No
Yes
Mmus\Lmntd1
1 of 15
No
Yes
Mmus\Lmntd2
1 of 15
No
Yes
Mmus\Nefl
1 of 15
No
Yes
Mmus\Nefm
1 of 15
No
Yes
Rattus norvegicus (Norway rat) (25)
Rnor\Lmnb2
12 of 13
Yes
Yes
Rnor\Lmna
11 of 13
No
Yes
Rnor\Lmnb1
11 of 13
No
Yes
Rnor\Des
2 of 13
No
Yes
Rnor\Vim
2 of 13
No
Yes
Rnor\Gfap
1 of 13
No
Yes
Rnor\Ina
1 of 13
No
Yes
Rnor\Krt4
1 of 13
No
Yes
Rnor\Krt5
1 of 13
No
Yes
Rnor\Krt8
1 of 13
No
Yes
Rnor\Krt13
1 of 13
No
Yes
Rnor\Krt14
1 of 13
No
Yes
Rnor\Krt15
1 of 13
No
Yes
Rnor\Krt16
1 of 13
No
Yes
Rnor\Krt17
1 of 13
No
Yes
Rnor\Krt19
1 of 13
No
Yes
Rnor\Krt20
1 of 13
No
Yes
Rnor\Krt35
1 of 13
No
Yes
Rnor\Krt36
1 of 13
No
Yes
Rnor\Krt75
1 of 13
No
Yes
Rnor\Lmntd1
1 of 13
No
Yes
Rnor\Lmntd2
1 of 13
No
Yes
Rnor\LOC683313
1 of 13
No
Yes
Rnor\Nefl
1 of 13
No
Yes
Rnor\Nefm
1 of 13
No
Yes
Xenopus tropicalis (Western clawed frog) (20)
Xtro\lmnb3
9 of 12
Yes
Yes
Xtro\lmnb2
7 of 12
No
Yes
Xtro\lmna
6 of 12
No
Yes
Xtro\lmnb1
6 of 12
No
Yes
Xtro\des.1
2 of 12
No
Yes
Xtro\vim
2 of 12
No
Yes
Xtro\ina
1 of 12
No
Yes
Xtro\krt7
1 of 12
No
Yes
Xtro\krt8.1
1 of 12
No
Yes
Xtro\krt17
1 of 12
No
Yes
Xtro\krt18.1
1 of 12
No
Yes
Xtro\krt19
1 of 12
No
Yes
Xtro\krt61
1 of 12
No
Yes
Xtro\krt70
1 of 12
No
Yes
Xtro\krt78.1
1 of 12
No
Yes
Xtro\krt78.7
1 of 12
No
Yes
Xtro\nefl
1 of 12
No
Yes
Xtro\nif
1 of 12
No
Yes
Xtro\prph
1 of 12
No
Yes
Xtro\vim.2
1 of 12
No
Yes
Danio rerio (Zebrafish) (29)
Drer\lmnb2
13 of 15
Yes
Yes
Drer\lmna
12 of 15
No
Yes
Drer\lmnl3
8 of 15
No
Yes
Drer\lmnb1
6 of 15
No
Yes
Drer\desma
2 of 15
No
Yes
Drer\desmb
2 of 15
No
Yes
Drer\vim
2 of 15
No
Yes
Drer\viml
2 of 15
No
Yes
Drer\cyt1
1 of 15
No
Yes
Drer\gfap
1 of 15
No
Yes
Drer\inaa
1 of 15
No
Yes
Drer\inab
1 of 15
No
Yes
Drer\krt4
1 of 15
No
Yes
Drer\krt5
1 of 15
No
Yes
Drer\krt8
1 of 15
No
Yes
Drer\krt15
1 of 15
No
Yes
Drer\krt18b
1 of 15
No
Yes
Drer\krt91
1 of 15
No
Yes
Drer\krt97
1 of 15
No
Yes
Drer\krt98
1 of 15
No
Yes
Drer\nefla
1 of 15
No
Yes
Drer\neflb
1 of 15
No
Yes
Drer\nefma
1 of 15
No
Yes
Drer\nefmb
1 of 15
No
Yes
Drer\ngs
1 of 15
No
Yes
Drer\prph
1 of 15
No
Yes
Drer\si:dkey-222n6.2
1 of 15
No
Yes
Drer\zgc:65851
1 of 15
No
Yes
Drer\zgc:172323
1 of 15
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (13)
Cele\lmn-1
11 of 15
Yes
Yes
Cele\ifa-1
7 of 15
No
Yes
Cele\ifa-3
6 of 15
No
Yes
Cele\ifb-1
6 of 15
No
Yes
Cele\ifa-4
5 of 15
No
Yes
Cele\mua-6
5 of 15
No
Yes
Cele\ifb-2
4 of 15
No
Yes
Cele\ifd-1
4 of 15
No
Yes
Cele\ifd-2
4 of 15
No
Yes
Cele\F10C1.8
3 of 15
No
Yes
Cele\ifc-1
3 of 15
No
Yes
Cele\ifc-2
3 of 15
No
Yes
Cele\ifp-1
2 of 15
No
Yes
Arabidopsis thaliana (thale-cress) (0)
No records found.
Saccharomyces cerevisiae (Brewer's yeast) (0)
No records found.
Schizosaccharomyces pombe (Fission yeast) (0)
No records found.
Other Organism Orthologs (via OrthoDB)
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (1)
Gene Symbol
Score
Source
Compara
eggNOG
Homologene
Inparanoid
Isobase
OrthoDB
OrthoFinder
orthoMCL
Panther
RoundUp
Alignment
LamC
9 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
Disease Model Summary Ribbon
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 4 )
Potential Models Based on Orthology ( 10 )
Modifiers Based on Experimental Evidence ( 1 )
Allele
Disease
Interaction
References
LamGL00577
exacerbates  tauopathy
modeled by Hsap\MAPTR406W.UAS
(Frost et al., 2016)
Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
Homo sapiens (Human)
Gene name
Score
OMIM
OMIM Phenotype
DO term
Complementation?
Transgene?
LMNA; lamin A/C
12 of 15
150330
 
LMNB2; lamin B2
12 of 15
150341
 
LMNB1; lamin B1
11 of 15
150340
 
Functional Complementation Data
Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
Interactions
Summary of Physical Interactions
esyN Network Diagram
Show neighbor-neighbor interactions:
Select Layout:
Legend:
Protein
RNA
Selected Interactor(s)
Interactions Browser
View in Interactions Browser
Please see the Physical Interaction reports below for full details
protein-protein
Physical Interaction
Assay
References
Lam
cosedimentation, western blot, inferred by author, two hybrid
(Zaremba-Czogalla et al., 2012, Schulze et al., 2009, Stuurman et al., 1999, Goldberg et al., 1998)
Lam - AGO2
anti bait coimmunoprecipitation, Identification by mass spectrometry
(Nazer et al., 2018)
Lam - BicD
two hybrid, pull down, western blot
(Stuurman et al., 1999)
Lam - Brca2
anti tag coimmunoprecipitation, western blot, ion exchange chromatography, peptide massfingerprinting, anti bait coimmunoprecipitation, pull down, anti tag western blot
(Kusch, 2015)
Lam - Dlc90F
experimental knowledge based
(Guruharsha et al., 2011)
Lam - JIL-1
anti bait coimmunoprecipitation, western blot, two hybrid, pull down
(Bao et al., 2005)
Lam - LBR
solid phase assay, anti bait coimmunoprecipitation, autoradiography, western blot
(Wagner et al., 2004)
Lam - LamC
two hybrid
(Schulze et al., 2009)
Lam - MAN1
anti bait coimmunoprecipitation, western blot, two hybrid
(Pinto et al., 2008, Wagner et al., 2006)
Lam - Mkrn1
experimental knowledge based
(Guruharsha et al., 2011)
Lam - Nup153
anti bait coimmunoprecipitation, western blot
(Chen et al., 2013)
Lam - Ote
two hybrid, experimental knowledge based, anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation
(Habermann et al., 2012, Guruharsha et al., 2011, Goldberg et al., 1998)
Lam - PIG-B
proximity-dependent biotin identification, western blot, coimmunoprecipitation, Identification by mass spectrometry, phenotype-based detection assay, inferred by author, anti tag coimmunoprecipitation
(Yamamoto-Hino et al., 2020)
Lam - Pen
affinity chromatography technology, Identification by mass spectrometry
(Virágh et al., 2012)
Lam - Polr2A
anti bait coimmunoprecipitation, Identification by mass spectrometry
(Nazer et al., 2018)
Lam - RPA1
experimental knowledge based
(Guruharsha et al., 2011)
Lam - RPA2
experimental knowledge based
(Guruharsha et al., 2011)
Lam - Rab5
affinity chromatography technology, Identification by mass spectrometry, pull down, western blot
(Capalbo et al., 2011)
Lam - Su(var)205
anti bait coimmunoprecipitation, western blot
(Napoletano et al., 2021)
Lam - Sumo
anti tag coimmunoprecipitation, comigration in sds page, anti tag western blot
(Pirone et al., 2017)
Lam - Topors
anti bait coimmunoprecipitation, western blot, two hybrid
(Capelson and Corces, 2005)
Lam - baf
anti tag coimmunoprecipitation, anti tag western blot
(Mehsen et al., 2018)
Lam - bocks
two hybrid, experimental knowledge based
(Guruharsha et al., 2011, Schulze et al., 2009)
Lam - cnn
anti bait coimmunoprecipitation, peptide massfingerprinting
(Habermann et al., 2012)
Lam - dod
anti bait coimmunoprecipitation, western blot, proximity ligation assay, fluorescence microscopy
(Napoletano et al., 2021)
Lam - fs(1)Ya
two hybrid
(Goldberg et al., 1998)
Lam - mud
anti bait coimmunoprecipitation, anti tag western blot, affinity chromatography technology, Identification by mass spectrometry
(Capalbo et al., 2011)
Lam - nxf2
anti bait coimmunoprecipitation, western blot
(Iwasaki et al., 2021)
Lam - p38a
anti tag coimmunoprecipitation, western blot
(Ryan et al., 2021)
Lam - sphe
experimental knowledge based
(Guruharsha et al., 2011)
Lam - stv
anti tag coimmunoprecipitation, western blot
(Ryan et al., 2021)
Lam - tws
anti tag coimmunoprecipitation, anti tag western blot
(Mehsen et al., 2018)
Lam - wash
proximity ligation assay, anti bait coimmunoprecipitation, western blot, blue native page, pull down, autoradiography
(Verboon et al., 2020, Verboon et al., 2015)
Lam - wge
pull down, western blot
(Masuko et al., 2018)
RNA-protein
Physical Interaction
Assay
References
Lam - nonA
anti bait coimmunoprecipitation, quantitative reverse transcription pcr
(Kozlova et al., 2006)
Summary of Genetic Interactions
esyN Network Diagram
esyN Network Key:
Suppression
Enhancement
View in Interactions Browser
Please look at the allele data for full details of the genetic interactions
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
Lam
suppressible
EcR
(Osouda et al., 2005)
Lam
suppressible
key
(Chen et al., 2014)
Lam
suppressible
LamC
(Chen et al., 2013)
Lam
suppressible
PGRP-LB|PGRP-SC2
(Chen et al., 2014)
Lam
suppressible
rl
(Chen et al., 2013)
Lam
suppressible
Su(var)205
(Chen et al., 2014)
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
hb
enhanceable
Lam
(Kohwi et al., 2013)
kay
suppressible
Lam|dia
(Belyaeva et al., 2022)
klar
enhanceable
Lam
(Patterson et al., 2004)
External Data
Subunit Structure (UniProtKB)
Interacts directly with LBR (PubMed:15054108). Interacts with MAN1 (PubMed:16439308). Interacts with Ote (PubMed:9632815, PubMed:22751930).
(UniProt, P08928 )
Linkouts
BioGRID - A database of protein and genetic interactions.
DroID - A comprehensive database of gene and protein interactions.
MIST (protein-protein) - An integrated Molecular Interaction Database
Pathways
Signaling Pathways (FlyBase)
Metabolic Pathways
FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
External Data
Linkouts
KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
Genomic Location and Detailed Mapping Data
Chromosome (arm)
2L
Recombination map
2-17
Cytogenetic map
25E6-25E6
Sequence location
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
25E6-25E6
Limits computationally determined from genome sequence between P{lacW}Hel25Ek11511 and P{lacW}l(2)k06502k06502
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
25E5-25E6
(determined by in situ hybridisation)
(Spradling et al., 1999)
25E4-25E6
25F1--2
(BDGP Project Members, 1994-1999)
25F1-25F2
(determined by in situ hybridisation)
(Gruenbaum et al., 1988)
Experimentally Determined Recombination Data
Location

2-17

(FlyBase, 2016)

2-18.5

(Comeron et al., 2012)
Left of (cM)
Right of (cM)
Notes
Stocks and Reagents
Stocks (21)
Genomic Clones (12)
 

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (105)
 

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced
BDGP DGC clones
Other clones
    Drosophila Genomics Resource Center cDNA clones

    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

    cDNA Clones, End Sequenced (ESTs)
    BDGP DGC clones
    Other clones
    RNAi and Array Information
    Linkouts
    DRSC - Results frm RNAi screens
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    Antibody Information
    Laboratory Generated Antibodies
     

    polyclonal

    (Petrovsky et al., 2018, Zaremba-Czogalla et al., 2012, Gurudatta et al., 2010, Wagner et al., 2004)

    monoclonal

    (Verboon et al., 2015)

    monoclonal antibody

    (Arama et al., 2003)
    Commercially Available Antibodies
     
    Developmental Studies Hybridoma Bank - Monoclonal antibodies for use in research
    Other Information
    Relationship to Other Genes
    Source for database identify of

    Source for identity of: Lam CG6944

    (Skupski, 1999.11)
    Source for database merge of

    Source for merge of: Lam l(2)04643

    (Spradling et al., 1999)

    Source for merge of: Lam misguided l(2)25Ec

    (Guillemin et al., 2001)

    Source for merge of: misguided l(2)25Ec

    (Guillemin, 2001.11.15)
    Additional comments
    Other Comments

    Lam is required in the cyst stem cell lineage but is dispensible in both the germline and the hub cells in the testis.

    (Chen et al., 2013)

    Lam is required to regulate organisation of the stem cell niche in the testis so that both the cyst stem cells and germline stem cells can assume proper interactions with the hub.

    (Chen et al., 2013)

    DNA-protein interactions: genome-wide binding profile assayed for Lam protein in Kc167 cells; see Chromatin_types_NKI collection report. Individual protein-binding experiments listed under "Samples" at GEO_GSE22069 (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE22069).

    (Filion et al., 2010)

    RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R+ cells.

    (Boutros et al., 2004)

    Lam is required for nuclear attachment to the microtubule organizing centre and for localization of klar to the nuclear envelope.

    (Patterson et al., 2004)

    Mutations in Lam disrupt the directed outgrowth of the cytoplasmic extensions from terminal cells of the tracheal system. Female germline mutant clones disrupt the dorsal-ventral polarity of the oocyte.

    (Guillemin et al., 2001)

    Lam protein interacts with both Ote and fs(1)Ya in a yeast two-hybrid system.

    (Goldberg et al., 1998)

    Identification: Enhancer trap expression pattern survey for loci expressed in the ring gland.

    (Harvie et al., 1998)

    Biochemical techniques reveal that in living cells lamins are closely associated with both DNA and RNA.

    (Rzepecki et al., 1998)

    The Lam gene product is essential for the structural integrity of the nuclear envelope and for the correct integration of nuclear pore complexes into the nuclear membrane.

    (Lenz-Bohme et al., 1997)

    Recruitment of fs(1)Ya and Lam to the male pronucleus occurs prior to or during the early stages of pronuclear migration and this accompanies or requires reorganisation of the sperm nucleus.

    (Liu et al., 1997)

    Region of a DNA fragment, λ20p1.4, which is a moderately repetitive sequence of the genome present in about 120 copies per haploid genome, polymerises with purified Lam.

    (Bogachev et al., 1996)

    The alpha-helical rod domain of Lam is sufficient for the specific interaction with the M/SAR fragment of ftz. For this specific interaction to occur it is essential that Lam is organised above the level of the dimer.

    (Zhao et al., 1996)

    Identified as being specifically expressed in the terminal cells of the developing tracheal system and required for the formation and outgrowth of the terminal branches.

    (Guillemin and Krasnow, 1995)

    Monoclonal antibody ADL84 is specific for Lam Dm1 and does not bind Lam Dm2. The antibody demonstrates that isolated interphase Lams Dm1 and Dm2 occur as mixed dimers, indicating random interactions. Mapping of the epitope for ADL84 shows Lam Dm2-specific phosphorylation takes place in the amino terminal head domain, most likely at Ser25.

    (Stuurman et al., 1995)

    Nuclear assembly has been followed in a cell free system: the Lam gene product plays essential role in nuclear envelope assembly.

    (Ulitzur et al., 1992)

    Three isoforms of Lam protein have been purified and their activity in vitro has been studied.

    (Lin and Fisher, 1990)

    Lam has been cloned and sequenced.

    (Osman et al., 1990)

    Some Lam protein is apparently retained in an envelope-like structure throughout mitosis in early Drosophila embryos.

    (Harel et al., 1989)

    A soluble isoform of Lam protein has been identified in the extracts of cultured cells blocked in mitosis. This isoform is the only species detectable in late-stage egg chambers and early embryos. The conversion of nuclear isoforms of Lam protein to this soluble isoform is mediated by a specific rearrangement of phosphate groups rather than large net changes in the levels of Lam protein phosphorylation. The residues involved in the phosphorylation/dephosphorylation reactions have been tentatively mapped within the fragment containing amino acids 385-547.

    (Smith and Fisher, 1989)

    l(2)25Ec was included as part of a study of the 24D4--25F2 region.

    (Szidonya and Reuter, 1988)

    Gene identified by screening a lambda-gt11 cDNA expression library, constructed from early embryonic mRNA, with monoclonal antibodies against Drosophila lamin. The structural gene for nuclear lamin. Translated as a prolamin of apparent molecular mass of 76kD, which is quickly processed to a 74kD species, presumably by proteolysis. The processed polypeptide is assembled into the nuclear envelope, where it becomes phosphorylated and again attains a mass of 76kD (Smith, Gruenbaum, Berrios, and Fisher, 1987).

    Origin and Etymology
    Discoverer
    Etymology

    The gene is named "misguided" because terminal branches of the tracheal system do not follow the normal patterns of projection during branch outgrowth in mutants.

    (Guillemin et al., 2001)
    Identification
    External Crossreferences and Linkouts ( 70 )
    Sequence Crossreferences
    NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
    GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
    GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
    RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
    UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
    UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
    UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
    Other crossreferences
    AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
    BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
    Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
    DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
    EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
    Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
    FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
    FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    Flygut - An atlas of the Drosophila adult midgut
    FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
    InterPro - A database of protein families, domains and functional sites
    KEGG Genes - Molecular building blocks of life in the genomic space.
    MARRVEL_MODEL - MARRVEL (model organism gene)
    modMine - A data warehouse for the modENCODE project
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DroID - A comprehensive database of gene and protein interactions.
    DRSC - Results frm RNAi screens
    Developmental Studies Hybridoma Bank - Monoclonal antibodies for use in research
    FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
    FlyMine - An integrated database for Drosophila genomics
    Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
    KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
    Synonyms and Secondary IDs (76)
    Reported As
    Symbol Synonym
    2459
    (Harvie et al., 1998)
    74/76
    (Filson et al., 1985)
    CG6944
    (Yamaguchi and Takashima, 2018, Kolkhof et al., 2017, Hosono et al., 2015, Aoki et al., 2012, Cammarato et al., 2011, Ni et al., 2011.7.19, Müller et al., 2010, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Hughes et al., 2008, Ni et al., 2008, Quinones-Coello, 2007, Stuart et al., 2007, Cermelli et al., 2006)
    D5
    (Stuurman et al., 1999)
    Dm0
    (Herrmann and Strelkov, 2011)
    DMO
    (Stuurman et al., 1996)
    Dm
    (Pałka et al., 2018, Rzepecki and Gruenbaum, 2018, Ulitzur et al., 1997)
    Dm(0)
    (Marshall, 2003)
    Dm0
    (Garreau-Balandier et al., 2014, Pindyurin et al., 2007, Brandt, 2006, Brandt et al., 2006, Pickersgill et al., 2006, Goldstein and Gunawardena, 2000, de Saint Phalle et al., 1999, de Saint Phalle et al., 1999, Berrios and Fisher, 1998, Stuurman, 1994.1.13)
    Dm1
    (Berrios and Fisher, 1998)
    Dm2
    (Berrios and Fisher, 1998)
    Dm0
    (Radermacher et al., 2014, Beard et al., 2008, Melcer et al., 2007, Schulze et al., 2005, Royzman et al., 2002, Loppin et al., 2001, Loppin et al., 2001, Wolfner and Wilson, 2001, Rzepecki and Fisher, 2000, Goldberg et al., 1999, Stuurman et al., 1999, Stuurman et al., 1999, Krohne et al., 1998, Rzepecki et al., 1998, Ashery-Padan et al., 1997, Lenz-Bohme et al., 1997, Sasse et al., 1997, Stuurman, 1997)
    Dm1
    (Rzepecki and Fisher, 2000, Rzepecki et al., 1998, Ulitzur et al., 1997, Lin and Fisher, 1990, Smith and Fisher, 1989)
    Dm2
    (Rzepecki and Fisher, 2000, Ulitzur et al., 1997, Lin and Fisher, 1990, Smith and Fisher, 1989)
    Dmmit
    (Rzepecki and Fisher, 2000, Lin and Fisher, 1990, Smith and Fisher, 1989)
    Dmo
    (Georgatos et al., 1994)
    DmLamin
    (Shareef et al., 2001)
    DmO
    (Polychronidou et al., 2010, Mills et al., 2006, Wagner et al., 2004, Kumar and Doumanis, 2000, Fraser et al., 1997)
    Dmo
    (Klapper et al., 1997)
    LAM
    (Llorens-Giralt et al., 2021, Pindyurin et al., 2016, Belyaeva et al., 2012, Filion et al., 2010, van Bemmel et al., 2010, van Steensel et al., 2010)
    Lam
    (Ábrahám et al., 2022, Belyaeva et al., 2022, Hafer et al., 2022, Pathak et al., 2022, Ryan et al., 2021, Yamaguchi et al., 2021, Bosch et al., 2020, Cavaliere et al., 2020, Kitani-Morii and Noto, 2020, Archambault, 2019.12.8, Bouska et al., 2019, Cornelison et al., 2019, Parey and Crombach, 2019, Yee et al., 2019, Zhao et al., 2019, Masuko et al., 2018, Yamaguchi and Takashima, 2018, Bussmann and Storkebaum, 2017, Fu et al., 2017, Pirone et al., 2017, Transgenic RNAi Project members, 2017-, Hosamani et al., 2016, Jana et al., 2016, Tran et al., 2016, Dietz et al., 2015, Chen et al., 2014, Kuang et al., 2014, Chen et al., 2013, Kwon et al., 2013, O'Sullivan et al., 2013, Taliaferro et al., 2013, Aoki et al., 2012, Habermann et al., 2012, Ranz et al., 2012, Timofeev et al., 2012, Belacortu et al., 2011, Friedman et al., 2011, Campbell et al., 2010, Kallappagoudar et al., 2010, Müller et al., 2010, Müller et al., 2010, Wagner et al., 2010, Mummery-Widmer et al., 2009, Shevelyov et al., 2009, de Wit et al., 2008, Bao et al., 2007, Buszczak et al., 2007, Kracklauer et al., 2007, Pindyurin et al., 2007, Quinones-Coello, 2007, Stuart et al., 2007, Christensen and Cook, 2006.7.11, Pickersgill et al., 2006, Schneider et al., 2006, Bao et al., 2005, Loop et al., 2004)
    Lam Dm0
    (Kim et al., 2015)
    Lam Dm0
    (Patterson et al., 2004)
    Lam(Dm0)
    (Kracklauer et al., 2007)
    LamDm0
    (Starr and Fischer, 2005)
    LamDm
    (Uchino et al., 2013, Onischenko et al., 2004)
    LamDm0
    (Ilyin et al., 2022, Flint Brodsly et al., 2019, Jokhi et al., 2013, Kim et al., 2012)
    LamDm0
    (Shevelyov et al., 2009, Furukawa et al., 2003)
    LamDmo
    (Mikhaylova and Nurminsky, 2011)
    Lamin
    (Warecki et al., 2020, Kusch, 2015, Matzat et al., 2012, Milon et al., 2012, Lancaster et al., 2010)
    Lamin A
    (Li et al., 2021)
    Lamin B
    (Milon et al., 2012, Brandt et al., 2008)
    Lamin Dm0
    (Yamamoto-Hino et al., 2020)
    Lamin Dm0
    (Adolphsen et al., 2012, Reim et al., 1999)
    Lamin-B1
    (Delandre and Marshall, 2019)
    LaminB
    (Nazer et al., 2018)
    jf27
    (Eberl et al., 1997, Szidonya and Reuter, 1988, Szidonya and Reuter, 1988)
    l(2)04643
    (Gildea et al., 2000, Meister and Braun, 1995.10, BDGP Project Members, 1994-1999)
    l(2)25Ec
    l(2)gdh-7
    (Kotarski et al., 1983)
    l(2)gdh7
    l(2)jf27
    lam
    (McLaughlin et al., 2021, Verboon et al., 2015, Markovic et al., 2009, Pavlovic et al., 2008, Muñoz-Alarcón et al., 2007, Cermelli et al., 2006, Munoz-Alarcon et al., 2006, Wilk et al., 2004, Maroni, 1994)
    lamB
    (Napoletano et al., 2021)
    lamDm0
    (Fischer et al., 2003)
    lamDm0
    (Gurudatta et al., 2010, Muñoz-Alarcón et al., 2007, Osouda et al., 2005)
    lamin
    (Lebo and McCall, 2021, Ambrosini et al., 2019, Dubois et al., 2016, Gistelinck et al., 2012, Hou et al., 2012, Milon et al., 2012, Doheny et al., 2008)
    lamin B
    (Brandt et al., 2008)
    lamin Dm0
    (Rohrbaugh et al., 2013, Wagner et al., 2004)
    lamin Dm0
    (Arama et al., 2003, Barkett et al., 2002, de Saint Phalle et al., 2002, Jackson et al., 2002, Cohen et al., 2001, Deng and Lin, 2001, Loppin et al., 2000, Guillemin et al., 1998, Riemer et al., 1995, Stuurman et al., 1995)
    lamin DmO
    (Dapergola et al., 2021, Sureka et al., 2018, Wagner et al., 2006)
    lamin-B
    (Liu and Jin, 2017, Karg et al., 2015)
    misg
    (Anonymous, 2001, Guillemin et al., 2001)
    nlam
    (Eberl et al., 1997)
    Name Synonyms
    B-type Lamin
    (Milon et al., 2012, Starr and Fischer, 2005)
    B-type lamin
    (Duan et al., 2020, Milon et al., 2012, Ranz et al., 2012, Pindyurin et al., 2007)
    Lamin
    (Dold et al., 2020, Yalonetskaya et al., 2020, Kolesnikova et al., 2018, Oyston et al., 2018, Petrovsky et al., 2018, Lakhotia, 2017, Pirone et al., 2017, Sopko et al., 2015, Kuang et al., 2014, Matzat and Lei, 2014, Minakhina et al., 2014, Nakayama et al., 2014, Radermacher et al., 2014, Radermacher et al., 2014, Sitaram et al., 2014, Zhang et al., 2014, Daigle et al., 2013, Deng and Kerppola, 2013, Anand and Kai, 2012, Belyaeva et al., 2012, Florentin and Arama, 2012, Gutiérrez et al., 2012, Kim et al., 2012, Loh et al., 2012, Murray et al., 2012, Nir et al., 2012, Belacortu et al., 2011, Benchabane et al., 2011, Cammarato et al., 2011, Chang et al., 2011, Chen et al., 2011, Jepson et al., 2011, Klenov et al., 2011, Schönbauer et al., 2011, Bulchand et al., 2010, Gerbasi et al., 2010, McPhee et al., 2010, Siegrist et al., 2010, Taniue et al., 2010, van Steensel et al., 2010, Wang et al., 2010, Stern et al., 2009, Vilmos et al., 2009, Cinnamon et al., 2008, Fisher et al., 2008, Meignin and Davis, 2008, Bao et al., 2007, Franklin-Dumont et al., 2007, Gawlinski et al., 2007, Kind and Akhtar, 2007, Ko et al., 2007, Kurshakova et al., 2007, Lancaster et al., 2007, Manak et al., 2007, Peretz et al., 2007, Sabri et al., 2007, Kozlova et al., 2006, Pickersgill et al., 2006, Pilot et al., 2006, Staudt et al., 2006, Capelson and Corces, 2005, Minakhina et al., 2005, Rollins et al., 2004)
    Lamin B
    (Mehta et al., 2020, Verboon et al., 2020, Pałka et al., 2018, Buster et al., 2013, Kalverda et al., 2010)
    Lamin D
    (Pak et al., 2011, Vrailas and Moses, 2006)
    Lamin Do
    (Bosveld et al., 2008)
    Lamin Dm0
    (Liu et al., 2013, Rohrbaugh et al., 2013, Zhang et al., 2013, Matsui et al., 2011, Matsui et al., 2007, Padiath et al., 2006, Pinto et al., 2006, Martin and Baehrecke, 2004, Smith et al., 2004)
    Lamin Dm0
    (Cheng et al., 2021, Dialynas et al., 2010, Gurudatta et al., 2010, Schulze et al., 2009, Schulze et al., 2008, Bass et al., 2007, Tanner et al., 2007)
    Lamin DmO
    (Creagh et al., 2009, Schwenkert et al., 2008)
    Lamin Dmo
    (Florentin and Arama, 2012, Zimek and Weber, 2011, Kessler and Müller, 2009)
    Lamin-B
    (Ojha and Tapadia, 2020, Civelekoglu-Scholey et al., 2010)
    LaminDm
    (Chang et al., 2011)
    LaminDm0
    (Gurudatta et al., 2012, Baxley et al., 2011, Urwyler et al., 2007)
    intermediate filament-like lamina
    (Heun et al., 2001)
    lamin
    (Munafò et al., 2021, Khoroshko et al., 2018, Kotov et al., 2016, Álvarez-Fernández et al., 2015, Garreau-Balandier et al., 2014, Neuman et al., 2014, Sanhueza et al., 2014, Zhang et al., 2014, Kohwi et al., 2013, Zhan et al., 2013, Kadir et al., 2012, Miguel et al., 2012, Olenkina et al., 2012, Wang et al., 2012, Yao et al., 2012, Barry et al., 2011, Hadjieconomou et al., 2011, Lanson et al., 2011, Maruyama et al., 2011, Desai et al., 2009, Häsemeyer et al., 2009, Kirchner et al., 2008, Oliver, 2008, Yapici et al., 2008, Berry and Baehrecke, 2007, Gawlinski et al., 2007, Peterson et al., 2007, Cermelli et al., 2006, Mills et al., 2006, Muraro and Moffat, 2006, Xylourgidis et al., 2006, Boykova et al., 2005, Crevel et al., 2005, Wilson, 2005, Daish et al., 2004, McHugh et al., 2004, Wagner et al., 2004, Fisher et al., 2001, Hutchison et al., 2001, Lee et al., 2000, Saunders and Cohen, 1999, Gigliotti et al., 1998, Goldberg et al., 1998, Likhacheva et al., 1998, Rzepecki et al., 1998, Lenz et al., 1995, Harel et al., 1989, Smith and Fisher, 1989)
    lamin A
    (Prokopenko et al., 1999)
    lamin B
    (Zhang et al., 2017, Herrmann and Strelkov, 2011, Wang et al., 2011, Lince-Faria et al., 2009, Brandt et al., 2008, Pathak et al., 2007, Brandt et al., 2006)
    lamin D0
    (Karsten et al., 2006)
    lamin Dm0
    (Herrmann and Strelkov, 2011)
    lamin Dm0
    (Graham et al., 2006)
    lamin Dm
    (Zaremba-Czogalla et al., 2011)
    lamin Dm0
    (Amiad-Pavlov et al., 2021, Dialynas et al., 2012, Virágh et al., 2012, Sharakhov et al., 2008, Wasser et al., 2007, Schulze et al., 2006, McHugh et al., 2004, Prufert et al., 2004, Prokopenko et al., 1999)
    lamin Dm0
    (Barton et al., 2014, Gurudatta et al., 2010, Furukawa et al., 2009, Pinto et al., 2008, Mattout et al., 2007, Melcer et al., 2007, Melcer and Gruenbaum, 2006, Bao et al., 2005, Mani et al., 2003)
    lamin Dm[[0]]
    (Bao et al., 2006)
    lamin-B
    (Chen et al., 2015)
    lethal(2)25Ec
    misguided
    (Anonymous, 2001, Guillemin et al., 2001, Guillemin, 1995)
    misrouted
    (Guillemin and Krasnow, 1995)
    n-lamin
    (Petersen et al., 1997)
    nuclear lamin
    (Martin et al., 2007, Xu et al., 2004, Baehrecke, 2003, Fischer et al., 2003)
    α-Lamin
    (Uv et al., 2000)
    Secondary FlyBase IDs
    • FBgn0001930
    • FBgn0010582
    • FBgn0014344
    Datasets (2)
    Study focus (2)
    Experimental Role
    Project
    Project Type
    Title
    • transgene_used
    FBGSE20313
    The insulator protein SU(HW) modulates nuclear lamina interactions of the Drosophila genome.
    • transgene_used
    FBGSE22069
    Protein profiling reveals five principal chromatin types in Drosophila cells.
    Study result (0)
    Result
    Result Type
    Title
    References (500)