FlyBase Gene Report: Dmel\lin

General Information

Symbol

Dmel\lin

Species

D. melanogaster

Name

lines

Annotation Symbol

CG11770

Feature Type

protein_coding_gene

FlyBase ID

FBgn0002552

Gene Model Status

Stock Availability

7 publicly available

Gene Summary

lines (lin) encodes a nuclear protein involved in developmental patterning and cell fate specification. The Odd-skipped family protein encoded by bowl binds to and destabilizes the product of lin in the nucleus. Converserly the Odd family protein encoded by drm stabilizes the product of bowl in the nucleus by outcompeting the binding of the product of lin to the product of bowl and localizing the product of lin to the cytoplasm. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
UniProtKB
Red Book
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

Sequence location

2R:8,914,524..8,918,234 [+]

Recombination map

2-59

RefSeq locus

NT_033778 REGION:8914524..8918234

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (13 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables protein binding

inferred from physical interaction with UniProtKB:Q9VQS6

(assigned by UniProt )

(Green et al., 2002)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (10 terms)

Terms Based on Experimental Evidence (10 terms)

CV Term

Evidence

References

involved_in embryonic foregut morphogenesis

inferred from mutant phenotype

(assigned by UniProt )

(Johansen et al., 2003)

involved_in embryonic hindgut morphogenesis

inferred from mutant phenotype

(assigned by UniProt )

(Johansen et al., 2003, Iwaki et al., 2001)

involved_in embryonic pattern specification

inferred from mutant phenotype

(assigned by UniProt )

(Iwaki et al., 2001, Bokor and DiNardo, 1996)

involved_in epidermis development

inferred from mutant phenotype

(Hatini et al., 2000)

involved_in foregut morphogenesis

inferred from mutant phenotype

(Green et al., 2002)

involved_in hindgut morphogenesis

inferred from mutant phenotype

(Green et al., 2002, Iwaki et al., 2001)

involved_in positive regulation of transcription by RNA polymerase II

inferred from mutant phenotype

(assigned by UniProt )

(Castelli-Gair, 1998)

involved_in proximal/distal axis specification

inferred from mutant phenotype

(Greenberg and Hatini, 2009)

involved_in spiracle morphogenesis, open tracheal system

inferred from mutant phenotype

(assigned by UniProt )

(Castelli-Gair, 1998)

involved_in wing disc development

inferred from mutant phenotype

(Nusinow et al., 2008)

Terms Based on Predictions or Assertions (0 terms)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

located_in cytoplasm

inferred from direct assay

(Hatini et al., 2000)

located_in nucleus

inferred from direct assay

(Hatini et al., 2000)

Terms Based on Predictions or Assertions (0 terms)

Gene Group (FlyBase)

Protein Family (UniProt)

Belongs to the protein lines family. (Q9V4Z9)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

lines (lin) encodes a nuclear protein involved in developmental patterning and cell fate specification. The Odd-skipped family protein encoded by bowl binds to and destabilizes the product of lin in the nucleus. Converserly the Odd family protein encoded by drm stabilizes the product of bowl in the nucleus by outcompeting the binding of the product of lin to the product of bowl and localizing the product of lin to the cytoplasm. [Date last reviewed: 2019-03-14]

Protein Function (UniProtKB)

Has a dual role as a segment polarity protein and as a modulator of the Abd-B protein. Required for Abd-B to activate the transcription of genes (including ems, cut and sal) that are involved in posterior spiracle morphogenesis. Also required for Abd-B to form an eighth abdominal denticle belt. Acts in a hierarchy downstream of drm and upstream of bowl during foregut and hindgut patterning and morphogenesis. Involved in cell rearrangement during elongation of the embryonic hindgut. Required to regulate expression of embryonic hindgut patterning genes in order to establish the large intestine and at least some rectum, and to repress small intestine fate. Required for late wingless (wg)-dependent cell fate specification in the dorsal embryonic epidermis. Acts in concert with wg to regulate expression of wg itself and also to regulate wg-target genes. May have a role in ventral epidermal patterning, independent of wg signaling.

(UniProt, Q9V4Z9)

Phenotypic Description (Red Book; Lindsley and Zimm 1992)

lin: lines (C. Nusslein-Volhard)

Embryonic lethal; small anterior portion of each segment deleted; A8, spiracles, and anal plates absent. Head abnormal.

Summary (Interactive Fly)

novel segment polarity gene - Lines is crucial for completing Wg signal transduction, acting downstream or in parallel to Armadillo

Interactive Fly

Gene Model and Products

Number of Transcripts

1

Number of Unique Polypeptides

1

Please see the JBrowse view of Dmel\lin for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.49

Sequence Ontology: Class of Gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

FBtr0088651

3654

858

Additional Transcript Data and Comments

Reported size (kB)

3.416 (longest cDNA)

(Hatini et al., 2000)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

FBpp0087732

96.3

858

4.96

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

858 (aa)

(Hatini et al., 2000)

Comments

External Data

Subunit Structure (UniProtKB)

Interacts with drm.

(UniProt, Q9V4Z9)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\lin using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

lin_1

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 10

embryonic hindgut

(Iwaki et al., 2001)

embryonic/larval midgut primordium | posterior

(Iwaki et al., 2001)

organism | ubiquitous

(Iwaki et al., 2001)

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

embryonic epidermis

(Hatini et al., 2000)

(Hatini et al., 2000)

(Hatini et al., 2000)

inclusive hindgut primordium

(Hatini et al., 2000)

Additional Descriptive Data

lin transcript is expressed widely, at low levels in the embryonic epidermis at the time of epidermal patterning and at higher levels in the hindgut and foregut primordia and in the mesoderm.

(Hatini et al., 2000)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

lin protein localization could not be determined with the anti-lin antibody, or with Myc-tagged lin, so localization was studied by overexpressing lin using the GAL4-UAS system. In 10 hr. embryos carrying "UAS-Lin" (lin^UAS.cHa) and the "Ptc-GAL4" driver (Scer\GAL4^ptc-559.1), lin protein localizes to the nucleus in cells anterior to the en domain, but localizes to the cytoplasm posterior to the en domain.

(Hatini et al., 2000)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in cytoplasm

inferred from direct assay

(Hatini et al., 2000)

located_in nucleus

inferred from direct assay

(Hatini et al., 2000)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\lin in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\lin

View exonic expression by tissue for Dmel\lin

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0002552

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG11770-RA

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG11770

Flygut - An atlas of the Drosophila adult midgut

FBgn0002552

Images

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 19 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 7 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of lin

Transgenic constructs containing regulatory region of lin

Aberrations (Deficiencies and Duplications) ( 13 )

Inferred from experimentation ( 13 )

Gene disrupted in

Gene not disrupted in

(Mohr and Boswell, 2002)

(Zheng et al., 2003)

(Mohr and Boswell, 2002)

(Mohr and Boswell, 2002)

(Mohr and Boswell, 2002)

Inferred from location ( 0 )

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^byn-Gal4

lin^UAS.cHa

lethal | recessive

lethal - all die before end of larval stage

lethal - all die during embryonic stage | recessive

lin^unspecified

partially lethal - majority die, with Scer\GAL4^pnr-MD237

lin^GD1479

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

lin^GD1479

viable, with Scer\GAL4^Mef2.PR

lin^GD1479

viable, with Scer\GAL4^tin.CΔ4

lin^GD1479

Other Phenotypes

Allele

abnormal size | embryonic stage

lin²

increased cell death, with Scer\GAL4^{Ubx-lac1-Gal4}

lin^UAS.cHa

increased cell number | embryonic stage

lin²

some die during embryonic stage | recessive

lin^unspecified

some die during larval stage

some die during pupal stage, with Scer\GAL4^pnr-MD237

lin^GD1479

visible, with Scer\GAL4^klu-G410

lin^UAS.cHa

visible, with Scer\GAL4^pnr-MD237

lin^GD1479

visible, with Scer\GAL4^{Ubx-lac1-Gal4}

lin^UAS.cHa

visible | pharate adult stage, with Scer\GAL4^dpp.blk1

lin^UAS.cHa

visible | somatic clone

lin^G2

visible | somatic clone, with Scer\GAL4^Act5C.PU

lin^UAS.cHa

Phenotype manifest in

Allele

abdominal segment 8

lin²

adult thorax, with Scer\GAL4^{Ubx-lac1-Gal4}

lin^UAS.cHa

adult thorax | somatic clone, with Scer\GAL4^Act5C.PU

lin^UAS.cHa

lin^G1

antenna | ectopic, with Scer\GAL4^dpp.blk1

lin^UAS.cHa

antenna | somatic clone

lin^G2

antennal disc, with Scer\GAL4^dpp.blk1

lin^UAS.cHa

arista, with Scer\GAL4^bab1-Agal4-5

lin^GD1479

arista, with Scer\GAL4^bab1-Pgal4-2

lin^GD1479

arista, with Scer\GAL4^dac-p7d23

lin^GD1479

basal cylinder, with Scer\GAL4^bab1-Agal4-5

lin^GD1479

basal cylinder, with Scer\GAL4^bab1-Pgal4-2

lin^GD1479

basal cylinder, with Scer\GAL4^dac-p7d23

lin^GD1479

basal cylinder, with Scer\GAL4^{rn-GAL4-DeltaS}

lin^GD1479

lin^unspecified

denticle | ectopic (with lin²)

lin^G2

denticle | ectopic (with lin^G2)

lin²

lin^G1

denticle belt (with lin²)

lin^G2

denticle belt (with lin^G2)

lin²

denticle belt & abdominal segment 8

lin^G1

denticle row 1 (with lin²)

lin^G2

denticle row 1 (with lin^G2)

lin²

denticle row 2 (with lin²)

lin^G2

denticle row 2 (with lin^G2)

lin²

denticle row 2 | ectopic, with Scer\GAL4^ptc-559.1, lin², lin^G2

lin^UAS.cHa

denticle row 2 | ectopic, with Scer\GAL4^ptc-559.1, lin², lin^UAS.cHa

lin^G2

denticle row 2 | ectopic, with Scer\GAL4^ptc-559.1, lin^G2, lin^UAS.cHa

lin²

denticle row 2 | ectopic, with Scer\GAL4^ptc-559.1, lin^G2/lin²

lin^UAS.cHa

denticle row 2 | ectopic (with lin²), with Scer\GAL4^ptc-559.1, lin^UAS.cHa

lin^G2

denticle row 2 | ectopic (with lin^G2), with Scer\GAL4^ptc-559.1, lin^UAS.cHa

lin²

denticle row 3 (with lin²)

lin^G2

denticle row 3 (with lin^G2)

lin²

denticle row 3 | ectopic, with Scer\GAL4^ptc-559.1, lin², lin^G2

lin^UAS.cHa

denticle row 3 | ectopic, with Scer\GAL4^ptc-559.1, lin², lin^UAS.cHa

lin^G2

denticle row 3 | ectopic, with Scer\GAL4^ptc-559.1, lin^G2, lin^UAS.cHa

lin²

denticle row 3 | ectopic, with Scer\GAL4^ptc-559.1, lin^G2/lin²

lin^UAS.cHa

denticle row 3 | ectopic (with lin²), with Scer\GAL4^ptc-559.1, lin^UAS.cHa

lin^G2

denticle row 3 | ectopic (with lin^G2), with Scer\GAL4^ptc-559.1, lin^UAS.cHa

lin²

denticle row 4 (with lin²)

lin^G2

denticle row 4 (with lin^G2)

lin²

disc epithelium proper & dorsal mesothoracic disc | somatic clone

lin^unspecified

dorsal denticle, with Scer\GAL4^ptc-559.1

lin^UAS.cHa

dorsal denticle | ectopic (with lin²)

lin^G2

dorsal denticle | ectopic (with lin^G2)

lin²

dorsal fine hair

lin²

dorsal fine hair | ectopic, with Scer\GAL4^ptc-559.1

lin^UAS.cHa

dorsal thick hair

lin²

dorsal thick hair, with Scer\GAL4^ptc-559.1

lin^UAS.cHa

embryonic/larval hindgut | embryonic stage

lin²

embryonic/larval posterior spiracle

embryonic/larval somatic muscle | dorsal

lin²

embryonic/larval spiracle primordium

lin^unspecified

embryonic abdominal segment 8

lin^unspecified

embryonic dorsal epidermis

lin^unspecified

embryonic dorsal epidermis, with Scer\GAL4^ptc-559.1

lin^UAS.cHa

embryonic epidermis | dorsal

lin^unspecified

embryonic epidermis | dorsal, with Scer\GAL4^ptc-559.1

lin^UAS.cHa

embryonic foregut

lin^unspecified

lin^unspecified

embryonic hindgut

embryonic hindgut, with Scer\GAL4^byn-Gal4

lin^UAS.cHa

embryonic large intestine

lin^unspecified

embryonic Malpighian tubule

lin²

embryonic proventriculus

lin^unspecified

embryonic segment | anterior

lin^unspecified

eye | somatic clone

lin^13B

eye disc | somatic clone, with Scer\GAL4^Act5C.PP

lin^UAS.cHa

head capsule, with Scer\GAL4^dpp.blk1

lin^UAS.cHa

hindgut proper primordium

lin^G1

keyhole structure

lin^unspecified

mesothoracic tergum, with Scer\GAL4^pnr-MD237

lin^GD1479

lin^G1

peripodial epithelium & dorsal mesothoracic disc, with Scer\GAL4^ptc-559.1

lin^UAS.cHa

peripodial epithelium & dorsal mesothoracic disc, with Scer\GAL4^{Ubx-lac1-Gal4}

lin^UAS.cHa

peripodial epithelium & dorsal mesothoracic disc | somatic clone, with Scer\GAL4^Act5C.PU

lin^UAS.cHa

photoreceptor cell | somatic clone

lin^13B

prescutum, with Scer\GAL4^klu-G410

lin^UAS.cHa

prothoracic tarsal segment, with Scer\GAL4^bab1-Agal4-5

lin^GD1479

prothoracic tarsal segment, with Scer\GAL4^bab1-Pgal4-2

lin^GD1479

prothoracic tarsal segment, with Scer\GAL4^dac-p7d23

lin^GD1479

prothoracic tarsal segment, with Scer\GAL4^Dll-md23

lin^GD1479

prothoracic tarsal segment, with Scer\GAL4^{rn-GAL4-DeltaS}

lin^GD1479

prothoracic tarsal segment | cell autonomous | somatic clone

lin^G2

sex comb, with Scer\GAL4^bab1-Agal4-5

lin^GD1479

sex comb, with Scer\GAL4^bab1-Pgal4-2

lin^GD1479

sex comb, with Scer\GAL4^dac-p7d23

lin^GD1479

sex comb, with Scer\GAL4^Dll-md23

lin^GD1479

sex comb, with Scer\GAL4^{rn-GAL4-DeltaS}

lin^GD1479

third segment of antenna, with Scer\GAL4^dac-p7d23

lin^GD1479

third segment of antenna, with Scer\GAL4^{rn-GAL4-DeltaS}

lin^GD1479

wing hinge primordium | somatic clone

lin^unspecified

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

4 of 15

Yes

Yes

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

4 of 15

Yes

Yes

Rattus norvegicus (Norway rat) (1)

4 of 13

Yes

Yes

Xenopus tropicalis (Western clawed frog) (1)

4 of 12

Yes

Yes

Danio rerio (Zebrafish) (1)

3 of 15

Yes

Yes

Caenorhabditis elegans (Nematode, roundworm) (0)

No records found.

Arabidopsis thaliana (thale-cress) (1)

1 of 9

Yes

Yes

Saccharomyces cerevisiae (Brewer's yeast) (0)

No records found.

Schizosaccharomyces pombe (Fission yeast) (0)

No records found.

Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091904RE )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila suzukii

Spotted wing Drosophila

Dsuz\DS10_00005854

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Drosophila persimilis

Drosophila willistoni

Drosophila virilis

Drosophila mojavensis

Drosophila grimshawi

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091501T2 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Glossina morsitans

Tsetse fly

Gmor\GMOY009105

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_09839

Mayetiola destructor

Hessian fly

Mdes\Mdes001177

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL010444

Anopheles darlingi

American malaria mosquito

Adar\ADAC003122

Anopheles gambiae

Malaria mosquito

Agam\AGAP010757

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ002947

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W03ZR )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA008201

Danaus plexippus

Monarch butterfly

Dple\DPOGS201641-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL015663

Apis florea

Little honeybee

Aflo\2511428372

Apis mellifera

Western honey bee

Bombus impatiens

Common eastern bumble bee

Bombus terrestris

Buff-tailed bumblebee

Linepithema humile

Argentine ant

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:000edc

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG000882

Tribolium castaneum

Red flour beetle

Pediculus humanus

Human body louse

Phum\PHUM584750

Rhodnius prolixus

Kissing bug

Rpro\RPRC009533

Cimex lectularius

Bed bug

Clec\CLEC005455

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI009023

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI061342

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_04787

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X03WE )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_305324

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G023I )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Strongylocentrotus purpuratus

Purple sea urchin

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000013653

Gallus gallus

Domestic chicken

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (0)

No records found.

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

LINS1; lines homolog 1

model of autosomal recessive non-syndromic intellectual disability

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

LINS1; lines homolog 1

4 of 15

MENTAL RETARDATION, AUTOSOMAL RECESSIVE 27; MRT27

autosomal recessive non-syndromic intellectual disability

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

coimmunoprecipitation, anti tag western blot, anti tag coimmunoprecipitation

(Del Signore et al., 2012, Hatini et al., 2005)

coimmunoprecipitation, anti tag western blot

(Del Signore et al., 2012)

coimmunoprecipitation, anti tag western blot

(Del Signore et al., 2012)

coimmunoprecipitation, anti tag western blot

(Del Signore et al., 2012)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

suppressible

(Castelli-Gair, 1998)

External Data

Subunit Structure (UniProtKB)

Interacts with drm.

(UniProt, Q9V4Z9 )

Linkouts

BioGRID - A database of protein and genetic interactions.

69592

DroID - A comprehensive database of gene and protein interactions.

FBgn0002552

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

45325

MIST (protein-protein) - An integrated Molecular Interaction Database

lin

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

2R

Recombination map

2-59

Cytogenetic map

Sequence location

2R:8,914,524..8,918,234 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

44F6-44F7

Limits computationally determined from genome sequence between P{lacW}Dmn^k16109&P{lacW}Gγ1^k08017 and P{lacW}babo^k16912

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

44F-44F

(Hatini, 2000.5.25)

Experimentally Determined Recombination Data

Location

2-59

(FlyBase, 2016, Tearle and Nusslein-Volhard, 1987)

2-58.0

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (7)

7074

w^*; P{UAS-lin.H}8

7087

w^*; lin^G2/CyO

50603

y¹ v¹; P{TRiP.HMC02375}attP2

40939

y¹ v¹; P{TRiP.HMS02187}attP40

3099

cn¹ lin² bw¹ speck¹/CyO

106840

cn¹ lin² bw¹ speck¹/CyO

v16801

w¹¹¹⁸; P{GD1479}v16801

Genomic Clones (13)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (30)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

LD43682

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0002552

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0002552

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

45325

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Hatini et al., 2000)

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for database merge of

Source for merge of: lin CG11770

(FlyBase, 1996-)

Additional comments

Source for merge of lin CG11770 was sequence comparison ( date:000622 ).

(FlyBase, 1996-)

Other Comments

lin and bowl act as a binary switch to specify disc proper and peripodial epithelium fates respectively during early development of the wing primordium.

(Nusinow et al., 2008)

lin is required for patterning of the hindgut.

(Iwaki et al., 2001)

lin is essential for wg-dependent patterning in the embryonic dorsal epidermis.

(Hatini et al., 2000)

Genetic evidence demonstrates that lin is required for the function of the Abd-B protein, but not for the function of other Hox genes. In mutant embryos Abd-B protein expression is normal but incapable of promoting its normal functions: formation of the posterior spiracle and specification of the eighth abdominal denticle belt.

(Castelli-Gair, 1998)

wg and hh signaling account for all cell types across the dorsal epidermis, where lin regulates the late expression of wg. lin function is antagonized by hh.

(Bokor and DiNardo, 1996)

lin is able to suppress some aspects of the late wg phenotype and later the identity of some hh-dependent cell types.

(Bokor et al., 1995)

The role of lin in the regulation of run mRNA expression in the early embryo has been investigated.

(Klingler and Gergen, 1993)

wg and en expression patterns are studied in all known segment polarity mutants to investigate the requirement of other segment polarity genes in mediating the maintenance of wg and en.

(van den Heuvel et al., 1993)

Zygotically active locus involved in the terminal developmental program in the embryo.

(Strecker et al., 1991)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 35 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

45325

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9V4Z9

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Q2XY68

Other crossreferences

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0002552

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

lin_1

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG11770

Flygut - An atlas of the Drosophila adult midgut

FBgn0002552

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

45325

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC011514

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG11770

modMine - A data warehouse for the modENCODE project

FBgn0002552

Linkouts

BioGRID - A database of protein and genetic interactions.

69592

DroID - A comprehensive database of gene and protein interactions.

FBgn0002552

DRSC - Results frm RNAi screens

FBgn0002552

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0002552

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG11770-RA

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0002552

FlyMine - An integrated database for Drosophila genomics

FBgn0002552

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

45325

MIST (protein-protein) - An integrated Molecular Interaction Database

lin

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

CG11770

(Hosono et al., 2015, Ni et al., 2010.12.1, Kim et al., 2004)

Lin

(Zoller and Schulz, 2012, Johansen et al., 2001)

lin

(Kittelmann et al., 2018, Aleksic et al., 2013, Zoller and Schulz, 2012, Zhai et al., 2010, Benítez et al., 2009, Mulinari and Häcker, 2009, Zhai et al., 2009, Brás-Pereira and Casares, 2008, Junion et al., 2007, Tao et al., 2007)

Name Synonyms

lines

(Wieschaus and Nüsslein-Volhard, 2016, Del Signore et al., 2012, Whitworth et al., 2012, Zoller and Schulz, 2012, Grusche et al., 2011, Zhai et al., 2010, Benítez et al., 2009, Greenberg and Hatini, 2009, Mulinari and Häcker, 2009, Zhai et al., 2009, Brás-Pereira and Casares, 2008, DiNardo et al., 2008, Nusinow et al., 2008, Hatini and Nusinow, 2007, Tao et al., 2007, Bras-Pereira et al., 2006, Waldrop et al., 2006)

Secondary FlyBase IDs

FBgn0033346

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (93)