FB2019_06, released Dec 19, 2019

Gene: Dmel\Lsp2

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General Information
Symbol
Dmel\Lsp2
Species
D. melanogaster
Name
Larval serum protein 2
Annotation Symbol
CG6806
Feature Type
protein_coding_gene
FlyBase ID
FBgn0002565
Gene Model Status
Current
Stock Availability
5 publicly available
Gene Snapshot
In progress.Contributions welcome.
Other Summaries
Also Known As
LSP-2, Pt-1, DmeLSP2
Key Links
Genomic Location
Cytogenetic map
68F5-68F5
Sequence location
3L:12,129,393..12,131,869 [+]
Recombination map
3-38
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Protein Family (UniProt)
Belongs to the hemocyanin family. (Q24388)
Protein Signatures (InterPro)
Molecular Function (GO)
[Detailed GO annotations]
Experimental Evidence
-
Predictions / Assertions
-
Summaries
Protein Function (UniProtKB)
Larval storage protein (LSP) which may serve as a store of amino acids for synthesis of adult proteins.
(UniProt, Q24388)
Phenotypic Description (Red Book; Lindsley and Zimm 1992)
Lsp2: Larval serum protein-2 (D.B. Roberts)
Codes for LSP2, a glycosylated polypeptide of 80,000 molecular weight (Brock and Roberts, 1980, Eur. J. Biochem. 106: 129-35) and one of the major serum proteins of third-instar larvae; a homohexamer of molecular weight 450,000 with subunit molecular weight of 78-83 X 103 daltons. LSP2 appears during the third larval instar and levels increase until midway through the pupal stage; then levels decline reaching base line early in adult life. Transcription detectable only in third-instar fat body, ecdysone dependent; in presence of temperature-sensitive ecd, Lsp2 transcription off at restrictive temperature; resumes following ecdysterone administration or shift to permissive temperature; juvenile hormone blocks ecdysterone stimulation.
Gene Model and Products
Number of Transcripts
2
Number of Unique Polypeptides
1

Please see the GBrowse view of Dmel\Lsp2 or the JBrowse view of Dmel\Lsp2 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model
Gene model reviewed during 5.56
Gene model reviewed during 5.46
Gene model reviewed during 5.43
Gene model reviewed during 6.02
Sequence Ontology: Class of Gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Lsp2-RA
FBtr0089324
NM_080077
2304
701
Lsp2-RB
FBtr0345352
NM_001300113
2477
701
Additional Transcript Data and Comments
Reported size (kB)
2.3 (longest cDNA)
(Mousseron-Grall et al., 1997)
2.3 (northern blot)
(Lepesant et al., 1982)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
Lsp2-PA
FBpp0088363
83.3
701
6.33
NP_524816
AAF49958
Lsp2-PB
FBpp0311507
83.3
701
6.33
NP_001287042
AHN58067
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

701 aa isoforms: Lsp2-PA, Lsp2-PB
Additional Polypeptide Data and Comments
Reported size (kDa)
718, 697 (aa); 85.5, 83.5 (kD predicted)
(Mousseron-Grall et al., 1997)
79 (kD)
(Lepesant et al., 1982)
Comments
External Data
Subunit Structure (UniProtKB)
Homohexamer.
(UniProt, Q24388)
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Nucleotide / Polypeptide Records
 
UniProt
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Lsp2 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Gene Ontology (3 terms)
Molecular Function (0 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (0 terms)
Biological Process (2 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
motor neuron axon guidance
inferred from mutant phenotype
(Inaki et al., 2007)
synaptic target inhibition
inferred from mutant phenotype
(Inaki et al., 2007)
Terms Based on Predictions or Assertions (0 terms)
Cellular Component (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
larval serum protein complex
inferred from direct assay
(Brock and Roberts, 1980)
Terms Based on Predictions or Assertions (0 terms)
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
No Assay Recorded
Stage
Tissue/Position (including subcellular localization)
Reference
second instar larval stage -- third instar larval stage
embryonic/larval fat body
(Powell et al., 1984)
microinjection
Stage
Tissue/Position (including subcellular localization)
Reference
third instar larval stage
(Lepesant et al., 1982)
radioisotope in situ
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head | restricted
(Benes et al., 1990)
Additional Descriptive Data
Lsp2 transcript accumulates in adults at 1% of the larval level. Eighty percent of adult Lsp2 expression localizes to the adipose tissue of the head. The larval and adult peptide sizes seem to differ, possibly as a result of glycosylation. Larval and adult Lsp2 transcripts have identical 5' ends, as judged by primer extension experiments.
(Benes et al., 1990)
The expression of Lsp1α, Lsp1β, Lsp1γ, and Lsp2 proteins and transcripts were compared by in vitro translation of mRNAs isolated from third instar larval fat body, and by Northern analysis of cytoplasmic and nuclear RNAs of second and third instar larval fat body. Lsp1α, Lsp1β, Lsp1γ, and Lsp2 proteins appear at the same time, and accumulate in coordinate fashion. The Lsp1α, Lsp1β, Lsp1γ, and Lsp2 transcripts appear at the same time (sometime after 46 hr) and accumulate in similar relative amounts. At 4 hr, transcript levels are at maximum, and all 4 polypeptides are synthesized at about equal amounts. However, Lsp1α, Lsp1β, and Lsp1γ proteins decline and are not synthesized at 92 hr, although there is still Lsp1α, Lsp1β, and Lsp1γ mRNA available at 96 hr. Lsp1α, Lsp1β and Lsp1γ subunit synthesis ceases before that of Lsp2 synthesis; the latter is no longer detected at 96 hr.
(Powell et al., 1984)
As deduced by in vitro translation of polyA RNA, Lsp2 is detected about 8 hours after the second larval molt and is present through third larval instar. Levels of this transcript increased in response to ecdysone supplement.
(Lepesant et al., 1982)
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
dissected tissue
Stage
Tissue/Position (including subcellular localization)
Reference
second instar larval stage -- third instar larval stage
embryonic/larval fat body
(Powell et al., 1984)
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Aradska et al., 2015)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
larval serum protein complex
inferred from direct assay
(Brock and Roberts, 1980)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Lsp2 in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 4 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 5 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Lsp2
Transgenic constructs containing regulatory region of Lsp2
Deletions and Duplications ( 13 )
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal | recessive
Other Phenotypes
Allele
visible, with Scer\GAL4da.G32
Phenotype manifest in
Allele
tergite, with Scer\GAL4da.G32
Orthologs
Downloads
Download All DIOPT Orthologs
Download All OrthoDB Orthologs
Human Orthologs (via DIOPT v7.1)
Homo sapiens (Human) (0)
No records found.
Model Organism Orthologs (via DIOPT v7.1)
Mus musculus (laboratory mouse) (0)
No records found.
Rattus norvegicus (Norway rat) (0)
No records found.
Xenopus tropicalis (Western clawed frog) (0)
No records found.
Danio rerio (Zebrafish) (0)
No records found.
Caenorhabditis elegans (Nematode, roundworm) (0)
No records found.
Arabidopsis thaliana (thale-cress) (0)
No records found.
Saccharomyces cerevisiae (Brewer's yeast) (0)
No records found.
Schizosaccharomyces pombe (Fission yeast) (0)
No records found.
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG091902U2 )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila melanogaster
fruit fly
Dmel\Lsp2
Drosophila suzukii
Spotted wing Drosophila
Dsuz\DS10_00004146
Drosophila simulans
Dsim\GD14358
Drosophila sechellia
Dsec\GM25327
Drosophila erecta
Dere\GG15559
Drosophila yakuba
Dyak\GE21884
Drosophila ananassae
Dana\GF23589
Drosophila pseudoobscura pseudoobscura
Dpse\GA24161
Drosophila persimilis
Dper\GL17278
Drosophila willistoni
Dwil\GK19679
Drosophila virilis
Dvir\GJ21616
Drosophila mojavensis
Dmoj\GI20647
Drosophila grimshawi
Dgri\GH21006
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091501S6 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Mdoa\17022421
Musca domestica
House fly
Mdoa\17022422
Musca domestica
House fly
Mdoa\17022418
Glossina morsitans
Tsetse fly
Gmor\GMOY005820
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_11773
Mayetiola destructor
Hessian fly
Mdes\Mdes010511
Mayetiola destructor
Hessian fly
Mdes\Mdes013072
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL011169
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL008817
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL013759
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL013757
Anopheles darlingi
American malaria mosquito
Adar\ADAC000944
Anopheles darlingi
American malaria mosquito
Adar\ADAC008484
Anopheles darlingi
American malaria mosquito
Adar\ADAC000943
Anopheles gambiae
Malaria mosquito
Agam\AGAP001345
Anopheles gambiae
Malaria mosquito
Agam\AGAP001659
Anopheles gambiae
Malaria mosquito
Agam\AGAP001657
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ001822
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ001820
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ007783
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W02LP )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bmor\BGIBMGA009027
Bombyx mori
Silkmoth
Bmor\BGIBMGA011266
Bombyx mori
Silkmoth
Bmor\BGIBMGA009026
Bombyx mori
Silkmoth
Bmor\BGIBMGA009028
Bombyx mori
Silkmoth
Bmor\BGIBMGA008859
Danaus plexippus
Monarch butterfly
Dple\DPOGS216053-PA
Danaus plexippus
Monarch butterfly
Dple\DPOGS209980-PA
Danaus plexippus
Monarch butterfly
Dple\DPOGS216049-PA
Heliconius melpomene
Postman butterfly
Hmel\HMEL015054
Heliconius melpomene
Postman butterfly
Hmel\HMEL015051
Heliconius melpomene
Postman butterfly
Hmel\HMEL015049
Heliconius melpomene
Postman butterfly
Hmel\HMEL009681
Apis florea
Little honeybee
Aflo\2430734996
Apis florea
Little honeybee
Aflo\2147647523
Apis mellifera
Western honey bee
Amel\Hex70c
Apis mellifera
Western honey bee
Amel\GB51698
Apis mellifera
Western honey bee
Amel\HEX70b
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP25108
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP25109
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP25110
Bombus terrestris
Buff-tailed bumblebee
Bter\15208199
Bombus terrestris
Buff-tailed bumblebee
Bter\15208198
Bombus terrestris
Buff-tailed bumblebee
Bter\15208196
Linepithema humile
Argentine ant
Lhum\LH22249
Linepithema humile
Argentine ant
Lhum\LH22251
Linepithema humile
Argentine ant
Lhum\LH22250
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:001e30
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:001e2f
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:001e2e
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG005332
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG005292
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG005330
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_07508
Tribolium castaneum
Red flour beetle
Tcas\TC005377
Tribolium castaneum
Red flour beetle
Tcas\TC006769
Tribolium castaneum
Red flour beetle
Tcas\TC005374
Tribolium castaneum
Red flour beetle
Tcas\TC005375
Tribolium castaneum
Red flour beetle
Tcas\TC005376
Tribolium castaneum
Red flour beetle
Tcas\TC006515
Pediculus humanus
Human body louse
Phum\PHUM494820
Pediculus humanus
Human body louse
Phum\PHUM598430
Rhodnius prolixus
Kissing bug
Rpro\RPRC001611
Rhodnius prolixus
Kissing bug
Rpro\RPRC007528
Cimex lectularius
Bed bug
Clec\CLEC002022
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_18795
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_18797
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_18796
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_04925
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X01IB )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Stegodyphus mimosarum
African social velvet spider
Smim\KFM59357.1
Tetranychus urticae
Two-spotted spider mite
Turt\tetur15g03490
Tetranychus urticae
Two-spotted spider mite
Turt\tetur16g02690
Tetranychus urticae
Two-spotted spider mite
Turt\tetur15g03620
Tetranychus urticae
Two-spotted spider mite
Turt\tetur15g03550
Tetranychus urticae
Two-spotted spider mite
Turt\tetur18g01970
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( None identified )
No non-Arthropod Metazoa orthologies identified
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v7.1)
Drosophila melanogaster (Fruit fly) (9)
Gene Symbol
Score
Source
Compara
eggNOG
Homologene
Inparanoid
Isobase
OrthoDB
OrthoFinder
orthoMCL
Panther
RoundUp
Alignment
Lsp1α
3 of 10
Lsp1β
3 of 10
Lsp1γ
3 of 10
CG7320
2 of 10
CG8100
2 of 10
Fbp1
2 of 10
PPO1
1 of 10
PPO2
1 of 10
PPO3
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    esyN Network Diagram
    Show neighbor-neighbor interactions:
    Select Layout:
    Legend:
    Protein
    RNA
    Selected Interactor(s)
    Interactions Browser
    View in Interactions Browser
    Please see the Physical Interaction reports below for full details
    protein-protein
    Physical Interaction
    Assay
    References
    Lsp2 - Adh
    experimental knowledge based
    (Guruharsha et al., 2011)
    Lsp2 - Ald1
    experimental knowledge based
    (Guruharsha et al., 2011)
    Lsp2 - Argk
    experimental knowledge based
    (Guruharsha et al., 2011)
    Lsp2 - CG16826
    experimental knowledge based
    (Guruharsha et al., 2011)
    Lsp2 - CG31198
    experimental knowledge based
    (Guruharsha et al., 2011)
    Lsp2 - CG31343
    experimental knowledge based
    (Guruharsha et al., 2011)
    Lsp2 - CG32473
    experimental knowledge based
    (Guruharsha et al., 2011)
    Lsp2 - CG34205
    experimental knowledge based
    (Guruharsha et al., 2011)
    Lsp2 - CG3776
    pull down, Identification by mass spectrometry
    (Liu et al., 2007)
    Lsp2 - CG4390
    experimental knowledge based
    (Guruharsha et al., 2011)
    Lsp2 - CG5500
    experimental knowledge based
    (Guruharsha et al., 2011)
    Lsp2 - CG5506
    experimental knowledge based
    (Guruharsha et al., 2011)
    Lsp2 - Cpr64Ac
    experimental knowledge based
    (Guruharsha et al., 2011)
    Lsp2 - Fer1HCH
    experimental knowledge based
    (Guruharsha et al., 2011)
    Lsp2 - Fer2LCH
    experimental knowledge based
    (Guruharsha et al., 2011)
    Lsp2 - Gapdh2
    experimental knowledge based
    (Guruharsha et al., 2011)
    Lsp2 - GlyP
    experimental knowledge based
    (Guruharsha et al., 2011)
    Lsp2 - Gpdh1
    experimental knowledge based
    (Guruharsha et al., 2011)
    Lsp2 - Nplp2
    experimental knowledge based
    (Guruharsha et al., 2011)
    Lsp2 - PPO1
    experimental knowledge based
    (Guruharsha et al., 2011)
    Lsp2 - Pgk
    experimental knowledge based
    (Guruharsha et al., 2011)
    Lsp2 - Pglym78
    experimental knowledge based
    (Guruharsha et al., 2011)
    Lsp2 - Scp1
    experimental knowledge based
    (Guruharsha et al., 2011)
    Lsp2 - Tsf1
    experimental knowledge based
    (Guruharsha et al., 2011)
    Lsp2 - Vajk2
    experimental knowledge based
    (Guruharsha et al., 2011)
    Lsp2 - Yp1
    experimental knowledge based
    (Guruharsha et al., 2011)
    Lsp2 - Yp2
    experimental knowledge based
    (Guruharsha et al., 2011)
    Lsp2 - Yp3
    experimental knowledge based
    (Guruharsha et al., 2011)
    Lsp2 - apolpp
    experimental knowledge based
    (Guruharsha et al., 2011)
    Summary of Genetic Interactions
    esyN Network Diagram
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    External Data
    Subunit Structure (UniProtKB)
    Homohexamer.
    (UniProt, Q24388 )
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DroID - A comprehensive database of gene and protein interactions.
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Pathways
    Signaling Pathways (FlyBase)
    Metabolic Pathways
    External Data
    Linkouts
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    3L
    Recombination map
    3-38
    Cytogenetic map
    68F5-68F5
    Sequence location
    3L:12,129,393..12,131,869 [+]
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    68F5-68F5
    Limits computationally determined from genome sequence between P{PZ}rols08232&P{lacW}l(3)j2D3j2D3 and P{PZ}l(3)0508805088
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    68E-68E
    (determined by in situ hybridisation)
    (Lepesant et al., 1982)
    68E-68F
    (determined by in situ hybridisation)
    (Levine et al., 1981)
    Experimentally Determined Recombination Data
    Location
    3-38
    (FlyBase, 2016)
    3-30.9
    (Comeron et al., 2012)
    3-37
    Left of (cM)
    Right of (cM)
    Notes
    3/17 of the distance from vin to gv.
    Stocks and Reagents
    Stocks (5)
    Genomic Clones (11)
     

    Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

    cDNA Clones (477)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequences
    BDGP DGC clones
    Other clones
      Drosophila Genomics Resource Center cDNA clones

      For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

      cDNA Clones, End Sequenced (ESTs)
      BDGP DGC clones
      Other clones
      RNAi and Array Information
      Linkouts
      DRSC - Results frm RNAi screens
      GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
      Antibody Information
      Laboratory Generated Antibodies
       
      Commercially Available Antibodies
       
      Other Information
      Relationship to Other Genes
      Source for database identify of
      Source for database merge of
      Source for merge of: Lsp2 CG6806
      (Misra, 2000.4.14)
      Additional comments
      The Lsp2 gene may have been derived from the Lsp1β gene by retroposition.
      (Pan and Zhang, 2009)
      Annotation CG11538 on opposite strand (eliminated in release 5.13) represents incorrect CDS.
      (FlyBase Genome Annotators, 2008)
      Other Comments
      P-element mediated germline transformation demonstrates a 230bp larval enhancer, which includes an ecdysone response element (EcRE), specifically targets Lsp2 gene activity to the larval fat body. Additional negative regulatory elements dictate both tissue-specificity and unique spatial restriction within the adult fat body.
      (Benes et al., 1996)
      Ecol\CAT and Ecol\lacZ reporter gene constructs carrying deletion mutants of the 5' flanking region transfected into Schneider cells confer transient ecdysone inducibility. A single functional ecdysone response element (EcRE) is localised at position -75 relative to the Lsp2 transcription initiation site. Structural features of this EcRE determine its ability to induce ecdysone responsiveness at a lower ligand concentration and may form the basis for differential hormone responsiveness within the fat body.
      (Antoniewski et al., 1995)
      Ecdysteroid-regulated gene.
      (Andres et al., 1993)
      Codes for Lsp2, a glycosylated polypeptide of 80,000 molecular weight (Brock and Roberts, 1980) and one of the major serum proteins of third instar larvae; a homohexamer of molecular weight 450,000 with subunit molecular weight of 78-83 x 103 daltons.
      (Lindsley and Zimm, 1992)
      Hydrazine is used to release oligosaccharides from a purified Lsp2 protein, sequence analysis is performed.
      (Williams et al., 1991)
      Origin and Etymology
      Discoverer
      Akam, 1977.
      Etymology
      Identification
      External Crossreferences and Linkouts ( 43 )
      Sequence Crossreferences
      NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
      GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
      GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
      RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
      UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
      UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
      Other crossreferences
      BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
      Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
      Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
      Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
      Flygut - An atlas of the Drosophila adult midgut
      GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
      iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
      InterPro - A database of protein families, domains and functional sites
      KEGG Genes - Molecular building blocks of life in the genomic space.
      modMine - A data warehouse for the modENCODE project
      Linkouts
      BioGRID - A database of protein and genetic interactions.
      DroID - A comprehensive database of gene and protein interactions.
      DRSC - Results frm RNAi screens
      FLIGHT - Cell culture data for RNAi and other high-throughput technologies
      FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
      FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
      FlyMine - An integrated database for Drosophila genomics
      InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
      MIST (protein-protein) - An integrated Molecular Interaction Database
      Synonyms and Secondary IDs (16)
      Reported As
      Symbol Synonym
      C23
      (Vierstraete et al., 2004)
      CG11538
      (FlyBase Genome Annotators, 2008)
      CG6806
      (Yadav et al., 2017, Çiçek et al., 2016, Palu and Thummel, 2016, Heinrichsen et al., 2014, McEachern et al., 2014, Ellis and Carney, 2011, Ellis and Carney, 2010, Ni et al., 2010.12.1, Shibata et al., 2010, Fujikawa et al., 2009, Keleman et al., 2009.8.5, Pan and Zhang, 2009, Perkins et al., 2009, Reumer et al., 2009, Vierstraete et al., 2004, Fulton et al., 2001)
      DmeLSP2
      (Burmester, 2001, Burmester et al., 1998)
      LHP
      LSP 2
      (Riddiford, 1993)
      LSP-2
      (Colomb et al., 2017, Burmester, 2000.3.29, Burmester et al., 1999, Roberts et al., 1998, Lepesant, 1996.5.9, Maschat et al., 1990, Deutsch et al., 1989, Hoogwerf et al., 1988, Powell et al., 1984, Sato et al., 1982, Yedvobnick and Levine, 1982)
      LSP2
      (Shibata and Kawabata, 2018, Liu et al., 2013, Shibata et al., 2010, Hoogwerf et al., 1988)
      Lsp-2
      (Parisi et al., 2010, Dauwalder et al., 2005, Zapata et al., 2002, Massey et al., 1997, Mousseron-Grall et al., 1997, Benes et al., 1996, Gordadze and Benes, 1996, Antoniewski et al., 1995, Andres et al., 1993, Benes et al., 1990)
      Lsp2
      (Kopp and Park, 2019, Kurshakova et al., 2019, Stobdan et al., 2019, Palmer et al., 2018, Wei et al., 2018, Zhang et al., 2018, Sun et al., 2017, Kučerová et al., 2016, Hamilton et al., 2015, Kim et al., 2015, Belozerov et al., 2014, Chatterjee et al., 2014, dos Santos, 2014, Lai et al., 2014, Mukherjee et al., 2014, Puerma et al., 2014, Ruiz et al., 2014, Whitaker et al., 2014, Handke et al., 2013, Liu et al., 2013, Short and Lazzaro, 2013, Wang et al., 2013, Nose, 2012, Ellis and Carney, 2011, Garcia et al., 2011, Bauer et al., 2010, Blanco et al., 2010, Dalton et al., 2010, Ellis and Carney, 2010, Fernández-Ayala et al., 2010, Kong et al., 2010, Fujikawa et al., 2009, Liu et al., 2009, Reumer et al., 2009, Sieber and Thummel, 2009, Xun et al., 2009, Juhász et al., 2008, Inaki et al., 2007, Lazareva et al., 2007, Beller et al., 2006, Hennig et al., 2006, Nelliot et al., 2006)
      Pt-1
      (Choudhary et al., 1992)
      Name Synonyms
      Larval serum protein 2
      (Lai et al., 2014, Liu et al., 2013, Zhan et al., 2013, Nose, 2012, Zhang et al., 2011, Parisi et al., 2010, Reumer et al., 2009, Xun et al., 2009, Xun et al., 2008)
      Larval serum protein-2
      (Antoniewski et al., 1995)
      Protein-1
      larval serum protein 2
      (McEachern et al., 2014, Robinson et al., 2010, Hennig et al., 2006)
      larval-hemolymph-protein
      Secondary FlyBase IDs
      • FBgn0036251
      Datasets (0)
      Study focus (0)
      Experimental Role
      Project
      Project Type
      Title
      References (137)