LSP-2, DmeLSP2, Pt-1
Please see the GBrowse view of Dmel\Lsp2 or the JBrowse view of Dmel\Lsp2 for information on other features
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Gene model reviewed during 5.56
Gene model reviewed during 5.46
Gene model reviewed during 5.43
Gene model reviewed during 6.02
2.3 (longest cDNA)
2.3 (northern blot)
718, 697 (aa); 85.5, 83.5 (kD predicted)
79 (kD)
Homohexamer.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Lsp2 using the Feature Mapper tool.
Lsp2 transcript accumulates in adults at 1% of the larval level. Eighty percent of adult Lsp2 expression localizes to the adipose tissue of the head. The larval and adult peptide sizes seem to differ, possibly as a result of glycosylation. Larval and adult Lsp2 transcripts have identical 5' ends, as judged by primer extension experiments.
The expression of Lsp1α, Lsp1β, Lsp1γ, and Lsp2 proteins and transcripts were compared by in vitro translation of mRNAs isolated from third instar larval fat body, and by Northern analysis of cytoplasmic and nuclear RNAs of second and third instar larval fat body. Lsp1α, Lsp1β, Lsp1γ, and Lsp2 proteins appear at the same time, and accumulate in coordinate fashion. The Lsp1α, Lsp1β, Lsp1γ, and Lsp2 transcripts appear at the same time (sometime after 46 hr) and accumulate in similar relative amounts. At 4 hr, transcript levels are at maximum, and all 4 polypeptides are synthesized at about equal amounts. However, Lsp1α, Lsp1β, and Lsp1γ proteins decline and are not synthesized at 92 hr, although there is still Lsp1α, Lsp1β, and Lsp1γ mRNA available at 96 hr. Lsp1α, Lsp1β and Lsp1γ subunit synthesis ceases before that of Lsp2 synthesis; the latter is no longer detected at 96 hr.
As deduced by in vitro translation of polyA RNA, Lsp2 is detected about 8 hours after the second larval molt and is present through third larval instar. Levels of this transcript increased in response to ecdysone supplement.
GBrowse - Visual display of RNA-Seq signals
View Dmel\Lsp2 in GBrowse 23-38
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Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for merge of: Lsp2 CG6806
Annotation CG11538 on opposite strand (eliminated in release 5.13) represents incorrect CDS.
P-element mediated germline transformation demonstrates a 230bp larval enhancer, which includes an ecdysone response element (EcRE), specifically targets Lsp2 gene activity to the larval fat body. Additional negative regulatory elements dictate both tissue-specificity and unique spatial restriction within the adult fat body.
Ecol\CAT and Ecol\lacZ reporter gene constructs carrying deletion mutants of the 5' flanking region transfected into Schneider cells confer transient ecdysone inducibility. A single functional ecdysone response element (EcRE) is localised at position -75 relative to the Lsp2 transcription initiation site. Structural features of this EcRE determine its ability to induce ecdysone responsiveness at a lower ligand concentration and may form the basis for differential hormone responsiveness within the fat body.
Ecdysteroid-regulated gene.
Codes for Lsp2, a glycosylated polypeptide of 80,000 molecular weight (Brock and Roberts, 1980) and one of the major serum proteins of third instar larvae; a homohexamer of molecular weight 450,000 with subunit molecular weight of 78-83 x 103 daltons.
Hydrazine is used to release oligosaccharides from a purified Lsp2 protein, sequence analysis is performed.
Akam, 1977.
