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Gene Dmel\Lsp2

General Information
Symbol	Dmel\Lsp2	Species	D. melanogaster
Name	Larval serum protein 2	Annotation symbol	CG6806
Feature type	protein_coding_gene	FlyBase ID	FBgn0002565
Gene Model Status	Current	Stock availability	1 publicly available
Also Known As	Lsp-2, DmeLSP2
Genomic Location
Chromosome (arm)	3L	Recombination map	3-37
Cytogenetic map	68F5-68F5	Sequence location	3L:12,122,493..12,124,796 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Larval serum protein 2 is referred to in FlyBase by the symbol Dmel\Lsp2 (CG6806, FBgn0002565). It is a protein_coding_gene from Drosophila melanogaster. It is reported to have molecular function: nutrient reservoir activity. The biological processes in which it is involved are not known. 7 alleles are reported. The phenotype of these alleles is annotated with: tergite. It has one annotated transcript and one annotated polypeptide. Protein features are: Hemocyanin, C-terminal; Hemocyanin, N-terminal; Hemocyanin/hexamerin; Hemocyanin/hexamerin middle domain; Immunoglobulin E-set; Uncharacterised domain, di-copper centre. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of extremely high expression to a trough of extremely low expression. Peak expression observed during late larval stages, at stages throughout the pupal period. Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: larval hindgut, larval Malpighian tubules, larval fat body, larval salivary gland, larval trachea, virgin spermatheca, larval/adult carcass. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, adult heart, adult fat body. Comments on Affy2 ProbeSet: ProbeSet 1641634_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of Lsp2. Gene sequence location is 3L:12122493..12124796.
User Contributed Data
	Beta version, feedback welcome
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	Lsp2: Larval serum protein-2 (D.B. Roberts) Codes for LSP2, a glycosylated polypeptide of 80,000 molecular weight (Brock and Roberts, 1980, Eur. J. Biochem. 106: 129-35) and one of the major serum proteins of third-instar larvae; a homohexamer of molecular weight 450,000 with subunit molecular weight of 78-83 X 103 daltons. LSP2 appears during the third larval instar and levels increase until midway through the pupal stage; then levels decline reaching base line early in adult life. Transcription detectable only in third-instar fat body, ecdysone dependent; in presence of temperature-sensitive ecd, Lsp2 transcription off at restrictive temperature; resumes following ecdysterone administration or shift to permissive temperature; juvenile hormone blocks ecdysterone stimulation.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 68F5-68F5   Limits computationally determined from genome sequence between P{PZ}rols08232&P{lacW}l(3)j2D3j2D3 and P{PZ}l(3)0508805088
Experimentally Determined Cytological Location
Cytogenetic map Notes References 68E-68E   (determined by in situ hybridisation)   (Lepesant et al., 1982) 68E-68F   (determined by in situ hybridisation)   (Levine et al., 1981)
Experimentally Determined Recombination Data
Location	3-37
Left of (cM)	gv
Right of (cM)	vin
Notes	3/17 of the distance from vin to gv.
Gene Model & Products
Please see the GBrowse view of Dmel\Lsp2 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.43 Gene model reviewed during 5.46 There is a 684aa open reading frame on the opposite strand from Lsp2.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Lsp2-RA FBtr0089324  NM_080077   2304   701
Additional Transcript Data & Comments
Reported size (kB)	2.3 (northern blot) (Lepesant et al., 1982) 2.3 (longest cDNA) (Mousseron-Grall et al., 1997)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Lsp2-PA   FBpp0088363   83.3   701   6.33     NP_524816   AAF49958
Additional Polypeptide Data & Comments
Reported size (kDa)	718, 697 (aa); 85.5, 83.5 (kD predicted) (Mousseron-Grall et al., 1997) 79 (kD) (Lepesant et al., 1982)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Hemocyanin/hexamerin middle domain (IPR000896) Hemocyanin, C-terminal (IPR005203) Hemocyanin, N-terminal (IPR005204) Uncharacterised domain, di-copper centre (IPR008922) Hemocyanin/hexamerin (IPR013788) Immunoglobulin E-set (IPR014756)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA567511   AE014296 AAF49958   AI256871   AI256878   AI256950   AI257024   AI257045   AI257068   AI257099   AI257143   AI257212   AI257234   AI257292   AI257320   AI257363   AI257453   AI257461   AI257543   AI257593   AI257622   AI257689   AI257768   AI257787   AI257806   AI258031   AI258065   AI258082   AI258095   AI258119   AI258152   AI258202   AI258225   AI258235   AI258306   AI258318   AI258362   AI258432   AI258468   AI258511   AI258539   AI258545   AI258657   AI258661   AI258677   AI258740   AI258771   AI258792   AI258798   AI258860   AI258885   AI258896   AI258904   AI258940   AI258976   AI259022   AI259045   AI259071   AI259085   AI259244   AI259263   AI259274   AI259291   AI259347   AI259406   AI259438   AI259551   AI259576   AI259705   AI259745   AI259748   AI259828   AI259850   AI259857   AI259867   AI259943   AI259944   AI260098   AI260186   AI260200   AI260232   AI260267   AI260306   AI260310   AI260327   AI260415   AI260457   AI260722   AI260836   AI260887   AI260894   AI260931   AI260955   AI260956   AI260982   AI261019   AI261041   AI261042   AI261137   AI261147   AI293369   AI293395   AI293419   AI293431   AI293469   AI293485   AI293588   AI293653   AI293707   AI293726   AI293730   AI293731   AI293738   AI293745   AI293845   AI293877   AI293905   AI293910   AI293923   AI293924   AI294053   AI294135   AI294138   AI294187   AI294249   AI294256   AI294437   AI294513   AI294572   AI294591   AI294643   AI294742   AI294772   AI294825   AI294841   AI294887   AI294894   AI294925   AI294976   AI295076   AI295108   AI295135   AI295152   AI295157   AI295163   AI295201   AI295223   AI295228   AI295372   AI295394   AI295422   AI295426   AI295428   AI295455   AI295530   AI295541   AI295565   AI295569   AI295582   AI295660   AI295661   AI295685   AI295707   AI295726   AI295796   AI295878   AI295901   AI295964   AI296038   AI296064   AI296126   AI296143   AI296145   AI296163   AI296166   AI296176   AI296220   AI296291   AI296297   AI296316   AI296416   AI296534   AI296542   AI296553   AI296652   AI296676   AI296684   AI296695   AI296703   AI296771   AI296785   AI296827   AI296839   AI296867   AI296919   AI297110   AI297143   AI297165   AI297181   AI297182   AI297183   AI297292   AI297399   AI297448   AI297486   AI297538   AI297566   AI297613   AI297628   AI297774   AI297810   AI297835   AI297885   AI297967   AY119005 AAM50865   BG639392   BI355909   BI355911   BI355919   BQ103012   CA803223   CA803365   CA803471   CA803606   CA803685   CA803766   CA803949   CA804124   CA804147   CA804222   CA804452   CA804608   CA804788   CA804939   CA806152   CA806262   CA806534   CA806740   CA806879   CA806985   CA807089   CA807179   CA807639   CA807646   CK131445   CK131540   CK131616   CK131623   CK131636   CK131660   CK131745   CK131747   CK131753   CK131770   CK131827   CK131844   CK131872   CK131929   CK131965   CK131994   CK132028   CK132050   CK132117   CK132144   CK132207   CK132276   CK132314   CK132337   CK132343   CK132348   CK132420   CK132427   CK132447   CK132485   CK445186   CK445194   CK657171   CK657180   CK657188   CK657190   CK657191   CK657194   CK657384   CK657534   CK657547   CK657573   CK657577   CK657622   CK657756   CK658011   CK658037   CK658059   CK658126   CK658129   CK658208   CK658231   CK658242   CK658329   CK658350   CK658354   CK658382   CK658532   CK658542   CK658558   CK658598   CK658800   CK658928   CK658955   CK659026   CK659031   CK659150   CK659169   CK659176   CK659218   CK659250   CK659328   CK659430   CK659455   CK659465   CK659532   CK659537   CK659572   CK659732   CK659739   CK659746   CK659808   CK659834   CK659855   CK659860   CK659867   CK659890   CK659976   CK659996   CK660049   CK660166   CK660260   CK660347   CK660379   CK660460   CK660513   CK660571   CK660600   CK660649   CK660668   CK660719   CK660723   CK660747   CK660779   CK660874   CK660897   CK660905   CK660920   CK661010   CK661070   CK661135   CK661170   CK661365   CK661391   CK661410   CK661471   CK661514   CK661577   CK661677   CK661738   CK661778   CK661798   CK661842   CK661862   CK661921   CK661948   CK661975   CK661999   CK662070   CK662444   CK662494   CO179794   CO179905   CO180096   CO180561   CO180589   CO180599   CO181166   CO181177   CO181189   CO181243   CO181247   CO181279   CO181296   CO181405   CO181415   CO181526   CO181672   CO181844   CO181874   CO181899   CO181925   CO182011   CO182105   CO182165   CO182191   CO182257   CO182449   CO182612   CO182784   CO182790   CO182805   CO183058   CO183082   CO183174   CO183235   CO183322   CO183422   CO183540   CO183638   CO183739   CO183810   CO184048   CO184054   CO184071   CO184090   CO184167   CO184185   CO184211   CO184231   CO184463   CO184622   CO184657   CO184718   CO184803   CO184886   CO184961   CO185140   CO185152   CO191230   CO191292   CO191388   CO191552   CO191648   CO191822   CO191895   CO191910   CO191930   CO191950   CO191964   CO191995   CO192229   CO192236   CO192470   CO192559   CO192564   CO192634   CO193004   CO193230   CO193416   CO193803   CO193994   CO194196   CO194317   CO194603   CO194687   CO194771   CO194842   CO194843   CO194967   CO195006   CO195114   CO195117   CO195221   CO195527   CO195531   CO195617   CO195853   CO195967   CO269572   EC082800   ES692211   ES692213   ES692214   ES692217   ES692220   ES692222   ES694720   ES695207   EV575820   EV581228   EV582902   EV589265   EV593842   X97770 CAA66371
UniProtKB/Swiss-Prot	Q24388
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
No Assay Recorded Stage Tissue/Position (including subcellular localization) Reference second instar larval stage -- third instar larval stage   embryonic/larval fat body   (Powell et al., 1984) microinjection Stage Tissue/Position (including subcellular localization) Reference third instar larval stage     (Lepesant et al., 1982) radioisotope in situ Stage Tissue/Position (including subcellular localization) Reference adult stage   adult head | restricted   (Benes et al., 1990)
Additional Descriptive Data
	The expression of Lsp1α, Lsp1β, Lsp1γ, and Lsp2 proteins and transcripts were compared by in vitro translation of mRNAs isolated from third instar larval fat body, and by Northern analysis of cytoplasmic and nuclear RNAs of second and third instar larval fat body. Lsp1α, Lsp1β, Lsp1γ, and Lsp2 proteins appear at the same time, and accumulate in coordinate fashion. The Lsp1α, Lsp1β, Lsp1γ, and Lsp2 transcripts appear at the same time (sometime after 46 hr) and accumulate in similar relative amounts. At 4 hr, transcript levels are at maximum, and all 4 polypeptides are synthesized at about equal amounts. However, Lsp1α, Lsp1β, and Lsp1γ proteins decline and are not synthesized at 92 hr, although there is still Lsp1α, Lsp1β, and Lsp1γ mRNA available at 96 hr. Lsp1α, Lsp1β and Lsp1γ subunit synthesis ceases before that of Lsp2 synthesis; the latter is no longer detected at 96 hr. (Powell et al., 1984) Lsp2 transcript accumulates in adults at 1% of the larval level. Eighty percent of adult Lsp2 expression localizes to the adipose tissue of the head. The larval and adult peptide sizes seem to differ, possibly as a result of glycosylation. Larval and adult Lsp2 transcripts have identical 5' ends, as judged by primer extension experiments. (Benes et al., 1990) As deduced by in vitro translation of polyA RNA, Lsp2 is detected about 8 hours after the second larval molt and is present through third larval instar. Levels of this transcript increased in response to ecdysone supplement. (Lepesant et al., 1982)
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
dissected tissue Stage Tissue/Position (including subcellular localization) Reference second instar larval stage -- third instar larval stage   embryonic/larval fat body   (Powell et al., 1984)
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
larval serum protein complex inferred from direct assay (Brock and Roberts, 1980) lipid particle inferred from direct assay (Beller et al., 2006)
Expression Deduced from Reporters
Reporter: P{Lsp2-GAL4.H} Stage Tissue/Position (including subcellular localization) Reference third instar larval stage   fat body   (Reiling and Hafen, 2004) wandering third instar larval stage   fat body   (Cherbas et al., 2003)
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: larval hindgut, larval Malpighian tubules, larval fat body, larval salivary gland, larval trachea, virgin spermatheca, larval/adult carcass. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, adult heart, adult fat body. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    19.6 Larval Midgut    52.9 Larval Hindgut    2843.3 Larval Malpighian Tubules    809.1 Larval Fat Body    10393.3 Larval Salivary Gland    6362 Larval Trachea    1004.175 Larval Carcass    1326.075 Adult Head    457.6 Adult Eye    481.75 Adult Brain    21.9 Adult Thoracic-Abdominal Ganglion    32.3 Adult Crop    15.3 Adult Midgut    19.4 Adult Hindgut    9.2 Adult Malpighian Tubules    10.5 Adult Fat Body    309.8 Adult Salivary Gland    21.2 Adult Heart    149.5 Adult VirginFemale Spermatheca    992.8 Adult InseminatedFemale Spermatheca    69 Adult Ovary    7 Adult Testis    9.6 Adult Male Accessory Gland    14 Adult Carcass    894.5 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    19.6 Larval Midgut    52.9 Larval Hindgut  (2843.3) Larval Malpighian Tubules  (809.1) Larval Fat Body  (10393.3) Larval Salivary Gland  (6362) Larval Trachea  (1004.175) Larval Carcass  (1326.075) Adult Head    457.6 Adult Eye    481.75 Adult Brain    21.9 Adult Thoracic-Abdominal Ganglion    32.3 Adult Crop    15.3 Adult Midgut    19.4 Adult Hindgut    9.2 Adult Malpighian Tubules    10.5 Adult Fat Body    309.8 Adult Salivary Gland    21.2 Adult Heart    149.5 Adult VirginFemale Spermatheca  (992.8) Adult InseminatedFemale Spermatheca    69 Adult Ovary    7 Adult Testis    9.6 Adult Male Accessory Gland    14 Adult Carcass  (894.5) Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    19.6 Larval Midgut    52.9 Larval Hindgut  (2843.3) Larval Malpighian Tubules    809.1 Larval Fat Body  (10393.3) Larval Salivary Gland  (6362) Larval Trachea  1004.175 Larval Carcass  (1326.075) Adult Head    457.6 Adult Eye    481.75 Adult Brain    21.9 Adult Thoracic-Abdominal Ganglion    32.3 Adult Crop    15.3 Adult Midgut    19.4 Adult Hindgut    9.2 Adult Malpighian Tubules    10.5 Adult Fat Body    309.8 Adult Salivary Gland    21.2 Adult Heart    149.5 Adult VirginFemale Spermatheca    992.8 Adult InseminatedFemale Spermatheca    69 Adult Ovary    7 Adult Testis    9.6 Adult Male Accessory Gland    14 Adult Carcass    894.5 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    19.6 Larval Midgut    52.9 Larval Hindgut    2843.3 Larval Malpighian Tubules    809.1 Larval Fat Body    10393.3 Larval Salivary Gland    6362 Larval Trachea    1004.175 Larval Carcass    1326.075 Adult Head    457.6 Adult Eye    481.75 Adult Brain    21.9 Adult Thoracic-Abdominal Ganglion    32.3 Adult Crop    15.3 Adult Midgut    19.4 Adult Hindgut    9.2 Adult Malpighian Tubules    10.5 Adult Fat Body    309.8 Adult Salivary Gland    21.2 Adult Heart    149.5 Adult VirginFemale Spermatheca    992.8 Adult InseminatedFemale Spermatheca    69 Adult Ovary    7 Adult Testis    9.6 Adult Male Accessory Gland    14 Adult Carcass    894.5 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    19.6 Larval Midgut    52.9 Larval Hindgut    2843.3 Larval Malpighian Tubules    809.1 Larval Fat Body    10393.3 Larval Salivary Gland    6362 Larval Trachea    1004.175 Larval Carcass    1326.075 Adult Head    457.6 Adult Eye    481.75 Adult Brain    21.9 Adult Thoracic-Abdominal Ganglion    32.3 Adult Crop    15.3 Adult Midgut    19.4 Adult Hindgut    9.2 Adult Malpighian Tubules    10.5 Adult Fat Body    309.8 Adult Salivary Gland    21.2 Adult Heart    149.5 Adult VirginFemale Spermatheca    992.8 Adult InseminatedFemale Spermatheca    69 Adult Ovary    7 Adult Testis    9.6 Adult Male Accessory Gland    14 Adult Carcass    894.5 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    19.6 Larval Midgut    52.9 Larval Hindgut  (2843.3) Larval Malpighian Tubules  809.1 Larval Fat Body  (10393.3) Larval Salivary Gland  (6362) Larval Trachea  (1004.175) Larval Carcass  (1326.075) Adult Head    457.6 Adult Eye    481.75 Adult Brain    21.9 Adult Thoracic-Abdominal Ganglion    32.3 Adult Crop    15.3 Adult Midgut    19.4 Adult Hindgut    9.2 Adult Malpighian Tubules    10.5 Adult Fat Body    309.8 Adult Salivary Gland    21.2 Adult Heart    149.5 Adult VirginFemale Spermatheca  (992.8) Adult InseminatedFemale Spermatheca    69 Adult Ovary    7 Adult Testis    9.6 Adult Male Accessory Gland    14 Adult Carcass  (894.5) Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    19.6 Larval Midgut    52.9 Larval Hindgut  (2843.3) Larval Malpighian Tubules    809.1 Larval Fat Body  (10393.3) Larval Salivary Gland  (6362) Larval Trachea  1004.175 Larval Carcass  1326.075 Adult Head    457.6 Adult Eye    481.75 Adult Brain    21.9 Adult Thoracic-Abdominal Ganglion    32.3 Adult Crop    15.3 Adult Midgut    19.4 Adult Hindgut    9.2 Adult Malpighian Tubules    10.5 Adult Fat Body    309.8 Adult Salivary Gland    21.2 Adult Heart    149.5 Adult VirginFemale Spermatheca    992.8 Adult InseminatedFemale Spermatheca    69 Adult Ovary    7 Adult Testis    9.6 Adult Male Accessory Gland    14 Adult Carcass    894.5 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    19.6 Larval Midgut    52.9 Larval Hindgut    2843.3 Larval Malpighian Tubules    809.1 Larval Fat Body    10393.3 Larval Salivary Gland    6362 Larval Trachea    1004.175 Larval Carcass    1326.075 Adult Head    457.6 Adult Eye    481.75 Adult Brain    21.9 Adult Thoracic-Abdominal Ganglion    32.3 Adult Crop    15.3 Adult Midgut    19.4 Adult Hindgut    9.2 Adult Malpighian Tubules    10.5 Adult Fat Body    309.8 Adult Salivary Gland    21.2 Adult Heart    149.5 Adult VirginFemale Spermatheca    992.8 Adult InseminatedFemale Spermatheca    69 Adult Ovary    7 Adult Testis    9.6 Adult Male Accessory Gland    14 Adult Carcass    894.5 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    457.6   NA  Eye    481.75   NA  Brain    no informative data   19.6  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    32.3   NA  Crop    no informative data   no informative data  Midgut    no informative data   2843.3  Hindgut    9.2   809.1  Malpighian Tubules    no informative data   10393.3  Fat Body    309.8   6362  Salivary Gland    no informative data   NA  Heart    149.5   1004.175  Trachea    NA   NA  VirginFemale Spermatheca    992.8   NA  InseminatedFemale Spermatheca    no informative data   NA  Ovary    7   NA  Testis    9.6   NA  Male Accessory Gland    no informative data   1326.075  Carcass    894.5 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    112 central nervous system, larvae L3    221 central nervous system, pupae P8    88 head, virgin 1-day female    1132 head, virgin 4-day female    213 head, virgin 20-day female    781 head, mated 1-day female    1302 head, mated 4-day female    729 head, mated 20-day female    1633 head, mated 1-day male    1136 head, mated 4-day male    918 head, mated 20-day male    515 salivary gland, larvae L3 wandering    1157 salivary gland, white prepupae    3701 digestive system, larvae L3 wandering    1273 digestive system, 1-day adult    22 digestive system, 4-day adult    15 digestive system, 20-day adult    5 fat body, larvae L3 wandering    40406 fat body, white prepupae    28144 fat body, pupae P8    15307 carcass, larvae L3 wandering    794 carcass, 1-day adult    1075 carcass, 4-day adult    420 carcass, 20-day adult    208 ovary, virgin 4-day female    4 ovary, mated 4-day female    0 testis, mated 4-day male    6 accessory gland, mated 4-day male    181 Expression Level Scale  Low   Moderate   Moderately high   High   Very high   Extremely high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (112) central nervous system, larvae L3  (221) central nervous system, pupae P8  (88) head, virgin 1-day female  (1132) head, virgin 4-day female  (213) head, virgin 20-day female  (781) head, mated 1-day female  (1302) head, mated 4-day female  (729) head, mated 20-day female  (1633) head, mated 1-day male  (1136) head, mated 4-day male  (918) head, mated 20-day male  (515) salivary gland, larvae L3 wandering  (1157) salivary gland, white prepupae  (3701) digestive system, larvae L3 wandering  (1273) digestive system, 1-day adult    22 digestive system, 4-day adult    15 digestive system, 20-day adult    5 fat body, larvae L3 wandering  (40406) fat body, white prepupae  (28144) fat body, pupae P8  (15307) carcass, larvae L3 wandering  (794) carcass, 1-day adult  (1075) carcass, 4-day adult  (420) carcass, 20-day adult  (208) ovary, virgin 4-day female    4 ovary, mated 4-day female    0 testis, mated 4-day male    6 accessory gland, mated 4-day male  (181) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (112) central nervous system, larvae L3  (221) central nervous system, pupae P8    88 head, virgin 1-day female  (1132) head, virgin 4-day female  (213) head, virgin 20-day female  (781) head, mated 1-day female  (1302) head, mated 4-day female  (729) head, mated 20-day female  (1633) head, mated 1-day male  (1136) head, mated 4-day male  (918) head, mated 20-day male  (515) salivary gland, larvae L3 wandering  (1157) salivary gland, white prepupae  (3701) digestive system, larvae L3 wandering  (1273) digestive system, 1-day adult    22 digestive system, 4-day adult    15 digestive system, 20-day adult    5 fat body, larvae L3 wandering  (40406) fat body, white prepupae  (28144) fat body, pupae P8  (15307) carcass, larvae L3 wandering  (794) carcass, 1-day adult  (1075) carcass, 4-day adult  (420) carcass, 20-day adult  (208) ovary, virgin 4-day female    4 ovary, mated 4-day female    0 testis, mated 4-day male    6 accessory gland, mated 4-day male  (181) Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    112 central nervous system, larvae L3    221 central nervous system, pupae P8    88 head, virgin 1-day female    1132 head, virgin 4-day female    213 head, virgin 20-day female    781 head, mated 1-day female    1302 head, mated 4-day female    729 head, mated 20-day female    1633 head, mated 1-day male    1136 head, mated 4-day male    918 head, mated 20-day male    515 salivary gland, larvae L3 wandering    1157 salivary gland, white prepupae    3701 digestive system, larvae L3 wandering    1273 digestive system, 1-day adult    22 digestive system, 4-day adult    15 digestive system, 20-day adult    5 fat body, larvae L3 wandering    40406 fat body, white prepupae    28144 fat body, pupae P8    15307 carcass, larvae L3 wandering    794 carcass, 1-day adult    1075 carcass, 4-day adult    420 carcass, 20-day adult    208 ovary, virgin 4-day female    4 ovary, mated 4-day female    0 testis, mated 4-day male    6 accessory gland, mated 4-day male    181 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    112 central nervous system, larvae L3    221 central nervous system, pupae P8    88 head, virgin 1-day female    1132 head, virgin 4-day female    213 head, virgin 20-day female    781 head, mated 1-day female    1302 head, mated 4-day female    729 head, mated 20-day female    1633 head, mated 1-day male    1136 head, mated 4-day male    918 head, mated 20-day male    515 salivary gland, larvae L3 wandering    1157 salivary gland, white prepupae    3701 digestive system, larvae L3 wandering    1273 digestive system, 1-day adult    22 digestive system, 4-day adult    15 digestive system, 20-day adult    5 fat body, larvae L3 wandering    40406 fat body, white prepupae    28144 fat body, pupae P8    15307 carcass, larvae L3 wandering    794 carcass, 1-day adult    1075 carcass, 4-day adult    420 carcass, 20-day adult    208 ovary, virgin 4-day female    4 ovary, mated 4-day female    0 testis, mated 4-day male    6 accessory gland, mated 4-day male    181 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (112) central nervous system, larvae L3  (221) central nervous system, pupae P8  (88) head, virgin 1-day female  (1132) head, virgin 4-day female  (213) head, virgin 20-day female  (781) head, mated 1-day female  (1302) head, mated 4-day female  (729) head, mated 20-day female  (1633) head, mated 1-day male  (1136) head, mated 4-day male  (918) head, mated 20-day male  (515) salivary gland, larvae L3 wandering  (1157) salivary gland, white prepupae  (3701) digestive system, larvae L3 wandering  (1273) digestive system, 1-day adult    22 digestive system, 4-day adult    15 digestive system, 20-day adult    5 fat body, larvae L3 wandering  (40406) fat body, white prepupae  (28144) fat body, pupae P8  (15307) carcass, larvae L3 wandering  (794) carcass, 1-day adult  (1075) carcass, 4-day adult  (420) carcass, 20-day adult  (208) ovary, virgin 4-day female    4 ovary, mated 4-day female    0 testis, mated 4-day male    6 accessory gland, mated 4-day male  (181) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering  112 central nervous system, larvae L3  (221) central nervous system, pupae P8    88 head, virgin 1-day female  (1132) head, virgin 4-day female  (213) head, virgin 20-day female  (781) head, mated 1-day female  (1302) head, mated 4-day female  (729) head, mated 20-day female  (1633) head, mated 1-day male  (1136) head, mated 4-day male  (918) head, mated 20-day male  (515) salivary gland, larvae L3 wandering  (1157) salivary gland, white prepupae  (3701) digestive system, larvae L3 wandering  (1273) digestive system, 1-day adult    22 digestive system, 4-day adult    15 digestive system, 20-day adult    5 fat body, larvae L3 wandering  (40406) fat body, white prepupae  (28144) fat body, pupae P8  (15307) carcass, larvae L3 wandering  (794) carcass, 1-day adult  (1075) carcass, 4-day adult  (420) carcass, 20-day adult  (208) ovary, virgin 4-day female    4 ovary, mated 4-day female    0 testis, mated 4-day male    6 accessory gland, mated 4-day male  (181) Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    112 central nervous system, larvae L3    221 central nervous system, pupae P8    88 head, virgin 1-day female    1132 head, virgin 4-day female    213 head, virgin 20-day female    781 head, mated 1-day female    1302 head, mated 4-day female    729 head, mated 20-day female    1633 head, mated 1-day male    1136 head, mated 4-day male    918 head, mated 20-day male    515 salivary gland, larvae L3 wandering    1157 salivary gland, white prepupae    3701 digestive system, larvae L3 wandering    1273 digestive system, 1-day adult    22 digestive system, 4-day adult    15 digestive system, 20-day adult    5 fat body, larvae L3 wandering    40406 fat body, white prepupae    28144 fat body, pupae P8    15307 carcass, larvae L3 wandering    794 carcass, 1-day adult    1075 carcass, 4-day adult    420 carcass, 20-day adult    208 ovary, virgin 4-day female    4 ovary, mated 4-day female    0 testis, mated 4-day male    6 accessory gland, mated 4-day male    181 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of extremely high expression to a trough of extremely low expression. Peak expression observed during late larval stages, at stages throughout the pupal period. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    1 embryo 08-10hr    1 embryo 10-12hr    1 embryo 12-14hr    1 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    2 embryo 20-22hr    0 embryo 22-24hr    1 larva L1    0 larva L2    0 larva L3 12hr old    143 larva L3 puffstage 1-2    14706 larva L3 puffstage 3-6    14119 larva L3 puffstage 7-9    7594 white prepupae new    5314 white prepupae 12hr    8007 white prepupae 24hr    4399 pupae 2d postWPP    2618 pupae 3d postWPP    1432 pupae 4d postWPP    582 adult male 01day    115 adult male 05day    30 adult male 30day    3 adult female 01day    62 adult female 05day    80 adult female 30day    39 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    1 embryo 08-10hr    1 embryo 10-12hr    1 embryo 12-14hr    1 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    2 embryo 20-22hr    0 embryo 22-24hr    1 larva L1    0 larva L2    0 larva L3 12hr old  (143) larva L3 puffstage 1-2  (14706) larva L3 puffstage 3-6  (14119) larva L3 puffstage 7-9  (7594) white prepupae new  (5314) white prepupae 12hr  (8007) white prepupae 24hr  (4399) pupae 2d postWPP  (2618) pupae 3d postWPP  (1432) pupae 4d postWPP  (582) adult male 01day  (115) adult male 05day  (30) adult male 30day    3 adult female 01day  (62) adult female 05day  (80) adult female 30day  (39) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    1 embryo 08-10hr    1 embryo 10-12hr    1 embryo 12-14hr    1 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    2 embryo 20-22hr    0 embryo 22-24hr    1 larva L1    0 larva L2    0 larva L3 12hr old  (143) larva L3 puffstage 1-2  (14706) larva L3 puffstage 3-6  (14119) larva L3 puffstage 7-9  (7594) white prepupae new  (5314) white prepupae 12hr  (8007) white prepupae 24hr  (4399) pupae 2d postWPP  (2618) pupae 3d postWPP  (1432) pupae 4d postWPP  (582) adult male 01day  (115) adult male 05day    30 adult male 30day    3 adult female 01day    62 adult female 05day    80 adult female 30day    39 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    1 embryo 08-10hr    1 embryo 10-12hr    1 embryo 12-14hr    1 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    2 embryo 20-22hr    0 embryo 22-24hr    1 larva L1    0 larva L2    0 larva L3 12hr old    143 larva L3 puffstage 1-2    14706 larva L3 puffstage 3-6    14119 larva L3 puffstage 7-9    7594 white prepupae new    5314 white prepupae 12hr    8007 white prepupae 24hr    4399 pupae 2d postWPP    2618 pupae 3d postWPP    1432 pupae 4d postWPP    582 adult male 01day    115 adult male 05day    30 adult male 30day    3 adult female 01day    62 adult female 05day    80 adult female 30day    39 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    1 embryo 08-10hr    1 embryo 10-12hr    1 embryo 12-14hr    1 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    2 embryo 20-22hr    0 embryo 22-24hr    1 larva L1    0 larva L2    0 larva L3 12hr old    143 larva L3 puffstage 1-2    14706 larva L3 puffstage 3-6    14119 larva L3 puffstage 7-9    7594 white prepupae new    5314 white prepupae 12hr    8007 white prepupae 24hr    4399 pupae 2d postWPP    2618 pupae 3d postWPP    1432 pupae 4d postWPP    582 adult male 01day    115 adult male 05day    30 adult male 30day    3 adult female 01day    62 adult female 05day    80 adult female 30day    39 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    1 embryo 08-10hr    1 embryo 10-12hr    1 embryo 12-14hr    1 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    2 embryo 20-22hr    0 embryo 22-24hr    1 larva L1    0 larva L2    0 larva L3 12hr old  (143) larva L3 puffstage 1-2  (14706) larva L3 puffstage 3-6  (14119) larva L3 puffstage 7-9  (7594) white prepupae new  (5314) white prepupae 12hr  (8007) white prepupae 24hr  (4399) pupae 2d postWPP  (2618) pupae 3d postWPP  (1432) pupae 4d postWPP  (582) adult male 01day  (115) adult male 05day  30 adult male 30day    3 adult female 01day  (62) adult female 05day  (80) adult female 30day  (39) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    1 embryo 08-10hr    1 embryo 10-12hr    1 embryo 12-14hr    1 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    2 embryo 20-22hr    0 embryo 22-24hr    1 larva L1    0 larva L2    0 larva L3 12hr old  143 larva L3 puffstage 1-2  (14706) larva L3 puffstage 3-6  (14119) larva L3 puffstage 7-9  (7594) white prepupae new  (5314) white prepupae 12hr  (8007) white prepupae 24hr  (4399) pupae 2d postWPP  (2618) pupae 3d postWPP  (1432) pupae 4d postWPP  (582) adult male 01day  115 adult male 05day    30 adult male 30day    3 adult female 01day    62 adult female 05day    80 adult female 30day    39 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    1 embryo 08-10hr    1 embryo 10-12hr    1 embryo 12-14hr    1 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    2 embryo 20-22hr    0 embryo 22-24hr    1 larva L1    0 larva L2    0 larva L3 12hr old    143 larva L3 puffstage 1-2    14706 larva L3 puffstage 3-6    14119 larva L3 puffstage 7-9    7594 white prepupae new    5314 white prepupae 12hr    8007 white prepupae 24hr    4399 pupae 2d postWPP    2618 pupae 3d postWPP    1432 pupae 4d postWPP    582 adult male 01day    115 adult male 05day    30 adult male 30day    3 adult female 01day    62 adult female 05day    80 adult female 30day    39 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    0 embryonic S1    0 embryonic S3    0 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    0 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    0 ovary fGS/OSS    0 ovary OSC    1 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    0 embryonic S1    0 embryonic S3    0 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    0 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    0 ovary fGS/OSS    0 ovary OSC    1 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    0 embryonic S1    0 embryonic S3    0 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    0 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    0 ovary fGS/OSS    0 ovary OSC    1 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    0 embryonic S1    0 embryonic S3    0 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    0 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    0 ovary fGS/OSS    0 ovary OSC    1 ovary OSS    0 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    0 embryonic S1    0 embryonic S3    0 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    0 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    0 ovary fGS/OSS    0 ovary OSC    1 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    0 embryonic S1    0 embryonic S3    0 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    0 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    0 ovary fGS/OSS    0 ovary OSC    1 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    0 embryonic S1    0 embryonic S3    0 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    0 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    0 ovary fGS/OSS    0 ovary OSC    1 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    0 embryonic S1    0 embryonic S3    0 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    0 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    0 ovary fGS/OSS    0 ovary OSC    1 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    4937 cold shock, 4-day adult    5976 heat shock, 4-day adult    2762 Cadmium 50 mM 6 hrs, larvae L3    7000 Cadmium 50 mM 12 hrs, larvae L3    8125 Cadmium 50 mM 48 hrs, 4-day adult    3109 Cadmium 100 mM 48 hrs, 4-day adult    3 Copper 0.5 mM 12 hrs, larvae L3    6431 Copper 15 mM 48 hrs, 4-day adult    840 Zinc 5 mM 12 hrs, larvae L3    5551 Zinc 4.5 mM 48 hrs, 4-day adult    1329 Ethanol 2.5% 3 hrs, larvae L3    16 Ethanol 5% 3 hrs, larvae L3    4077 Ethanol 10% 3 hrs, larvae L3    6067 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5439 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    12 Caffeine 25 mg/ml 48 hrs, 4-day adult    4541 Paraquat 5 mM 48 hrs, 4-day adult    3 Paraquat 10 mM 48 hrs, 4-day adult    12 Rotenone 2 μg 12 hrs, larvae L3    5368 Rotenone 8 μg 12 hrs, larvae L3    3607 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  (4937) cold shock, 4-day adult  (5976) heat shock, 4-day adult  (2762) Cadmium 50 mM 6 hrs, larvae L3  (7000) Cadmium 50 mM 12 hrs, larvae L3  (8125) Cadmium 50 mM 48 hrs, 4-day adult  (3109) Cadmium 100 mM 48 hrs, 4-day adult    3 Copper 0.5 mM 12 hrs, larvae L3  (6431) Copper 15 mM 48 hrs, 4-day adult  (840) Zinc 5 mM 12 hrs, larvae L3  (5551) Zinc 4.5 mM 48 hrs, 4-day adult  (1329) Ethanol 2.5% 3 hrs, larvae L3    16 Ethanol 5% 3 hrs, larvae L3  (4077) Ethanol 10% 3 hrs, larvae L3  (6067) Caffeine 1.5 mg/ml 4 hrs, larvae L3  (5439) Caffeine 2.5 mg/ml 48 hrs, 4-day adult    12 Caffeine 25 mg/ml 48 hrs, 4-day adult  (4541) Paraquat 5 mM 48 hrs, 4-day adult    3 Paraquat 10 mM 48 hrs, 4-day adult    12 Rotenone 2 μg 12 hrs, larvae L3  (5368) Rotenone 8 μg 12 hrs, larvae L3  (3607) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult  (4937) cold shock, 4-day adult  (5976) heat shock, 4-day adult  (2762) Cadmium 50 mM 6 hrs, larvae L3  (7000) Cadmium 50 mM 12 hrs, larvae L3  (8125) Cadmium 50 mM 48 hrs, 4-day adult  (3109) Cadmium 100 mM 48 hrs, 4-day adult    3 Copper 0.5 mM 12 hrs, larvae L3  (6431) Copper 15 mM 48 hrs, 4-day adult  (840) Zinc 5 mM 12 hrs, larvae L3  (5551) Zinc 4.5 mM 48 hrs, 4-day adult  (1329) Ethanol 2.5% 3 hrs, larvae L3    16 Ethanol 5% 3 hrs, larvae L3  (4077) Ethanol 10% 3 hrs, larvae L3  (6067) Caffeine 1.5 mg/ml 4 hrs, larvae L3  (5439) Caffeine 2.5 mg/ml 48 hrs, 4-day adult    12 Caffeine 25 mg/ml 48 hrs, 4-day adult  (4541) Paraquat 5 mM 48 hrs, 4-day adult    3 Paraquat 10 mM 48 hrs, 4-day adult    12 Rotenone 2 μg 12 hrs, larvae L3  (5368) Rotenone 8 μg 12 hrs, larvae L3  (3607) Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    4937 cold shock, 4-day adult    5976 heat shock, 4-day adult    2762 Cadmium 50 mM 6 hrs, larvae L3    7000 Cadmium 50 mM 12 hrs, larvae L3    8125 Cadmium 50 mM 48 hrs, 4-day adult    3109 Cadmium 100 mM 48 hrs, 4-day adult    3 Copper 0.5 mM 12 hrs, larvae L3    6431 Copper 15 mM 48 hrs, 4-day adult    840 Zinc 5 mM 12 hrs, larvae L3    5551 Zinc 4.5 mM 48 hrs, 4-day adult    1329 Ethanol 2.5% 3 hrs, larvae L3    16 Ethanol 5% 3 hrs, larvae L3    4077 Ethanol 10% 3 hrs, larvae L3    6067 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5439 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    12 Caffeine 25 mg/ml 48 hrs, 4-day adult    4541 Paraquat 5 mM 48 hrs, 4-day adult    3 Paraquat 10 mM 48 hrs, 4-day adult    12 Rotenone 2 μg 12 hrs, larvae L3    5368 Rotenone 8 μg 12 hrs, larvae L3    3607 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    4937 cold shock, 4-day adult    5976 heat shock, 4-day adult    2762 Cadmium 50 mM 6 hrs, larvae L3    7000 Cadmium 50 mM 12 hrs, larvae L3    8125 Cadmium 50 mM 48 hrs, 4-day adult    3109 Cadmium 100 mM 48 hrs, 4-day adult    3 Copper 0.5 mM 12 hrs, larvae L3    6431 Copper 15 mM 48 hrs, 4-day adult    840 Zinc 5 mM 12 hrs, larvae L3    5551 Zinc 4.5 mM 48 hrs, 4-day adult    1329 Ethanol 2.5% 3 hrs, larvae L3    16 Ethanol 5% 3 hrs, larvae L3    4077 Ethanol 10% 3 hrs, larvae L3    6067 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5439 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    12 Caffeine 25 mg/ml 48 hrs, 4-day adult    4541 Paraquat 5 mM 48 hrs, 4-day adult    3 Paraquat 10 mM 48 hrs, 4-day adult    12 Rotenone 2 μg 12 hrs, larvae L3    5368 Rotenone 8 μg 12 hrs, larvae L3    3607 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  (4937) cold shock, 4-day adult  (5976) heat shock, 4-day adult  (2762) Cadmium 50 mM 6 hrs, larvae L3  (7000) Cadmium 50 mM 12 hrs, larvae L3  (8125) Cadmium 50 mM 48 hrs, 4-day adult  (3109) Cadmium 100 mM 48 hrs, 4-day adult    3 Copper 0.5 mM 12 hrs, larvae L3  (6431) Copper 15 mM 48 hrs, 4-day adult  (840) Zinc 5 mM 12 hrs, larvae L3  (5551) Zinc 4.5 mM 48 hrs, 4-day adult  (1329) Ethanol 2.5% 3 hrs, larvae L3    16 Ethanol 5% 3 hrs, larvae L3  (4077) Ethanol 10% 3 hrs, larvae L3  (6067) Caffeine 1.5 mg/ml 4 hrs, larvae L3  (5439) Caffeine 2.5 mg/ml 48 hrs, 4-day adult    12 Caffeine 25 mg/ml 48 hrs, 4-day adult  (4541) Paraquat 5 mM 48 hrs, 4-day adult    3 Paraquat 10 mM 48 hrs, 4-day adult    12 Rotenone 2 μg 12 hrs, larvae L3  (5368) Rotenone 8 μg 12 hrs, larvae L3  (3607) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult  (4937) cold shock, 4-day adult  (5976) heat shock, 4-day adult  (2762) Cadmium 50 mM 6 hrs, larvae L3  (7000) Cadmium 50 mM 12 hrs, larvae L3  (8125) Cadmium 50 mM 48 hrs, 4-day adult  (3109) Cadmium 100 mM 48 hrs, 4-day adult    3 Copper 0.5 mM 12 hrs, larvae L3  (6431) Copper 15 mM 48 hrs, 4-day adult  (840) Zinc 5 mM 12 hrs, larvae L3  (5551) Zinc 4.5 mM 48 hrs, 4-day adult  (1329) Ethanol 2.5% 3 hrs, larvae L3    16 Ethanol 5% 3 hrs, larvae L3  (4077) Ethanol 10% 3 hrs, larvae L3  (6067) Caffeine 1.5 mg/ml 4 hrs, larvae L3  (5439) Caffeine 2.5 mg/ml 48 hrs, 4-day adult    12 Caffeine 25 mg/ml 48 hrs, 4-day adult  (4541) Paraquat 5 mM 48 hrs, 4-day adult    3 Paraquat 10 mM 48 hrs, 4-day adult    12 Rotenone 2 μg 12 hrs, larvae L3  (5368) Rotenone 8 μg 12 hrs, larvae L3  (3607) Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    4937 cold shock, 4-day adult    5976 heat shock, 4-day adult    2762 Cadmium 50 mM 6 hrs, larvae L3    7000 Cadmium 50 mM 12 hrs, larvae L3    8125 Cadmium 50 mM 48 hrs, 4-day adult    3109 Cadmium 100 mM 48 hrs, 4-day adult    3 Copper 0.5 mM 12 hrs, larvae L3    6431 Copper 15 mM 48 hrs, 4-day adult    840 Zinc 5 mM 12 hrs, larvae L3    5551 Zinc 4.5 mM 48 hrs, 4-day adult    1329 Ethanol 2.5% 3 hrs, larvae L3    16 Ethanol 5% 3 hrs, larvae L3    4077 Ethanol 10% 3 hrs, larvae L3    6067 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5439 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    12 Caffeine 25 mg/ml 48 hrs, 4-day adult    4541 Paraquat 5 mM 48 hrs, 4-day adult    3 Paraquat 10 mM 48 hrs, 4-day adult    12 Rotenone 2 μg 12 hrs, larvae L3    5368 Rotenone 8 μg 12 hrs, larvae L3    3607 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_04
External Data & Images
Linkouts	BDGP expression data • LP03332 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0002565 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG11538-RA;CG6806-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | recessive Lsp2F Other Phenotypes Allele visible, with Scer\GAL4da.G32 Lsp2NIG.6806R Phenotype manifest in Allele tergite, with Scer\GAL4da.G32 Lsp2NIG.6806R
Classical Alleles ( 4 )
For All Classical Alleles Show
Allele of Lsp2 Class Mutagen Stocks Known lesion Lsp2G19255 P-element activity 1 -- Lsp25 natural population 0 -- Lsp2F natural population 0 -- Lsp2S 0 --
Alleles Carried on Transgenic Constructs ( 3 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Lsp2 Class Mutagen Stocks Known lesion Lsp2KK115182 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes Lsp2NIG.6806R in vitro construct - regulatory fusion in vitro construct - RNAi 0 Yes Lsp2tHa in vitro construct - genomic fragment 0 --
Aneuploid Aberrations
Disrupted in	Df(3L)Exel6116 (Ryder, 2004.4.26) Df(3L)vin4 (Hoogwerf et al., 1988) Df(3L)vin5 (Hoogwerf et al., 1988) Df(3L)vin6 (Hoogwerf et al., 1988) Df(3L)vin7 (Hoogwerf et al., 1988) Df(3L)vin8 (Hoogwerf et al., 1988) Df(3L)vin9 (Hoogwerf et al., 1988)
Not disrupted in	Df(3L)P20 (Hoogwerf et al., 1988) Df(3L)vin2 (Hoogwerf et al., 1988) Df(3L)vin3 (Hoogwerf et al., 1988) Df(3L)vin10 (Hoogwerf et al., 1988) Df(3L)vin11 (Hoogwerf et al., 1988) Df(3L)vin66 (Hoogwerf et al., 1988)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data GAL4 construct P{0.68Lsp2-Gal4} No P{3.1Lsp2-Gal4} No reporter construct P{0.23Lsp-2-hsp-lacZ} No P{0.28Lsp-2-lacZ} No P{0.64Lsp-2-hsp-lacZ} No P{0.68Lsp-2-lacZ} No P{0.126Lsp-2-hsp-lacZ} No P{1.5Lsp-2-lacZ} No P{3.1Lsp-2-lacZ} No UAS construct P{KK115182} NA characterization construct P{LSP2} NA P{Lsp2-GAL4.H} NA P{NIG.6806R} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EP}Lsp2G19255 NA
Gene Ontology: Function, Process & Cellular Component ( 4 unique terms )
Terms Based on Experimental Evidence ( 2 terms )
Molecular Function ( 0 terms)
Biological Process ( 0 terms)
Cellular Component
CV term Evidence References
larval serum protein complex inferred from direct assay (Brock and Roberts, 1980) lipid particle inferred from direct assay (Beller et al., 2006)
Terms Based on Predictions or Assertions ( 2 terms )
Molecular Function
CV term Evidence References
nutrient reservoir activity non-traceable author statement (Swiss-Prot Project Members, 1997.2.1)
Biological Process ( 0 terms)
Cellular Component
CV term Evidence References
extracellular region non-traceable author statement (Swiss-Prot Project Members, 1997.2.1)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
Lsp2 - Adh experimental knowledge based (Guruharsha et al., 2011) Lsp2 - Ald experimental knowledge based (Guruharsha et al., 2011) Lsp2 - Argk experimental knowledge based (Guruharsha et al., 2011) Lsp2 - CG10581 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Lsp2 - CG16826 experimental knowledge based (Guruharsha et al., 2011) Lsp2 - CG16885 experimental knowledge based (Guruharsha et al., 2011) Lsp2 - CG31198 experimental knowledge based (Guruharsha et al., 2011) Lsp2 - CG31343 experimental knowledge based (Guruharsha et al., 2011) Lsp2 - CG32473 experimental knowledge based (Guruharsha et al., 2011) Lsp2 - CG34205 experimental knowledge based (Guruharsha et al., 2011) Lsp2 - CG4390 experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) Lsp2 - CG5500 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) Lsp2 - CG5506 experimental knowledge based (Guruharsha et al., 2011) Lsp2 - Cpr64Ac experimental knowledge based (Guruharsha et al., 2011) Lsp2 - Fer1HCH experimental knowledge based (Guruharsha et al., 2011) Lsp2 - Fer2LCH experimental knowledge based (Guruharsha et al., 2011) Lsp2 - Gapdh2 experimental knowledge based (Guruharsha et al., 2011) Lsp2 - GlyP experimental knowledge based (Guruharsha et al., 2011) Lsp2 - Gpdh experimental knowledge based (Guruharsha et al., 2011) Lsp2 - Nplp2 experimental knowledge based (Guruharsha et al., 2011) Lsp2 - Pgk experimental knowledge based (Guruharsha et al., 2011) Lsp2 - Pglym78 experimental knowledge based (Guruharsha et al., 2011) Lsp2 - Rfabg experimental knowledge based (Guruharsha et al., 2011) Lsp2 - Scp1 experimental knowledge based (Guruharsha et al., 2011) Lsp2 - Tsf1 experimental knowledge based (Guruharsha et al., 2011) Lsp2 - Yp1 experimental knowledge based (Guruharsha et al., 2011) Lsp2 - Yp2 experimental knowledge based (Guruharsha et al., 2011) Lsp2 - Yp3 experimental knowledge based (Guruharsha et al., 2011) Lsp2 - proPO-A1 experimental knowledge based (Guruharsha et al., 2011)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Lsp2 allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 69593 DroID - A comprehensive database of gene and protein interactions. • FBgn0002565 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 45326
Orthologs
OrthoDB Orthologs (26) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6CZC1F Dipteran inclusive ortholog search EOG641QKK Insect inclusive ortholog search No orthologs identified Arthropod inclusive ortholog search No orthologs identified Metazoa inclusive ortholog search No orthologs identified
Orthologs in Drosophila Species (EOG6CZC1F)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Lsp2     Drosophila simulans   Dsim\GD14358 Y   Drosophila sechellia   Dsec\GM25327 Y   Drosophila erecta   Dere\GG15559 Y   Drosophila yakuba   Dyak\GE21884 Y   Drosophila ananassae   Dana\GF23589 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA24161 Y   Drosophila persimilis   Dper\GL17278 Y   Drosophila willistoni   Dwil\GK19679 Y   Drosophila virilis   Dvir\GJ21616 Y   Drosophila mojavensis   Dmoj\GI20647 Y   Drosophila grimshawi   Dgri\GH21006 Y
Orthologs in non-Drosophila Dipterans (EOG641QKK)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL008817   Aedes aegypti Yellow fever mosquito  Aaeg\AAEL013983   Aedes aegypti Yellow fever mosquito  Aaeg\AAEL011169   Aedes aegypti Yellow fever mosquito  Aaeg\AAEL013981   Aedes aegypti Yellow fever mosquito  Aaeg\AAEL013759   Aedes aegypti Yellow fever mosquito  Aaeg\AAEL013757   Anopheles gambiae Malaria mosquito  Agam\AGAP001345   Anopheles gambiae Malaria mosquito  Agam\AGAP001659   Anopheles gambiae Malaria mosquito  Agam\AGAP001657   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ001822   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ001820   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ007783   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ006537   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ009033
Orthologs in non-Dipteran Insects (None identified)
No non-Dipteran orthologies identified
Orthologs in non-Insect Arthropods (None identified)
No non-Insect Arthropod orthologies identified
Orthologs in non-Arthropod Metazoa (None identified)
No non-Arthropod Metazoa orthologies identified
Human Orthologs (0)
Gene OMIM HGNC
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD14358 Drosophila sechellia Dsec\GM25327 Drosophila erecta Dere\GG15559 Drosophila yakuba Dyak\GE21884 Drosophila ananassae Dana\GF23589 Drosophila pseudoobscura pseudoobscura Dpse\GA24161 Drosophila persimilis Dper\GL17278 Drosophila willistoni Dwil\GK19679 Drosophila virilis Dvir\GJ21616 Drosophila mojavensis Dmoj\GI20647 Drosophila grimshawi Dgri\GH21006
Stocks & Reagents
Stocks Listed in FlyBase ( 1 )
Bloomington	27451 y1 w*; P{EP}Lsp2G19255
Genomic Clones ( 2 )
	BACR05P17 BACR14K14 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 477 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LP03332
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	LP01176 LP01338 LP01459 LP01633 LP01767 LP02156 LP02195 LP02368 LP02395 LP02672 LP02739 LP02851 LP03019 LP03366 LP03889 LP04090 LP04267 LP04329 LP04880 LP05110 LP05273 LP05427 LP05542 LP05608 LP05643 LP06133 LP06260 LP06645 LP06906 LP06949 LP07086 LP07507 LP07812 LP07908 LP08055 LP08061 LP08414 LP08474 LP08644 LP08714 LP08828 LP09010 LP09053 LP09208 LP09255 LP09351 LP09867 LP09968 LP10059 LP10865 LP10921 LP11747 LP11790 LP11860 LP11966 LP12146 LP12455 LP12526 LP12541 LP12577 LP12742 LP12847 LP12910 LP13088 LP13142 LP13380 LP13434 LP13549 LP13677 LP13788 LP13948 LP14291 LP14310 LP14586 LP14719 LP15218 LP15222 LP15511 LP15566 LP15642 LP16445 LP16459 LP16739 LP16748 LP16832 LP16877 LP17183 LP17247 LP17353 LP17362 LP17427 LP17901 LP17949 LP17971 LP18156 LP18676 LP19067 LP19434 LP19582 LP20864 LP21001 LP21175 LP21528 LP21590 LP21843 LP22019 LP22679 LP23285 LP23937 LP23981
Other clones	CK01.132A.A10 Dmel_dig_003635_0665_3557 Dmel_dig_015196_0595_3335 Dmel_dig_019385_0663_2892 Dmel_neb_014583_0356_1869 Dmel_neb_015388_0635_1349 EC00217 EC00491 EC00891 EC02054 EC02137 EC02393 EC13675 EC13701 EC13730 EC13853 EC13864 EC13948 EC13990 EC14250 EC14268 EC14479 EC14821 EC15084 EC15151 EC15403 EC17208 EC21596 EC22109 EC22194 EC22237 EC22338 EC22708 EC23409 EC24022 EC24030 EC24048 EC24487 EC24533 EC25039 EC25229 EC25459 EC25708 EC25933 EC26075 EC26236 EC26445 EC27001 EC27015 EC27041 EC27087 EC27261 EC27303 EC27370 EC27417 EC28128 EC28335 EC28377 EC28465 EC28605 EC28739 EC28937 EC29709 EC29733 EC30488 EC30638 EC30793 EC31386 EC31596 EC32185 EC32363 EC32391 EC32434 EC32474 EC32501 EC32546 EC33391 EC33410 EC33816 EC34057 EC34069 EC34185 EC34972 EC35327 EC35752 EC36827 EC37236 EC37587 EC38013 EC38883 EC39040 EC39194 EC39326 EC39328 EC39532 EC39577 EC39726 EC39730 EC39882 EC40526 EC40530 EC40675 EC41088 EC43760 EK095033 ESG011a.E7.4_H03 ESG011a.E21_H03 ESG011d.E7.1_B02 ESG011d.E21_B02 ESG012a.E21_H02 ESG012c.E21_C11 ESG013d.E21_C12 ESG014d.E21_D02 ESG015b.E21_G12 ESG015d.E21.1_D04 ESG016b.E21_A03 ESG016c.E21_E06 ESG018d.E21_C05 ESG018d.E21_D05 ESG0110a.E21_E03 ESG0110b.E21_H12 ESG0111b.B21.1_G11 ESG0112a.B21_H10 ESG0112b.B21_C10 ESG0112c.B21_F02 ESG0113c.B21.1_F05 ESG0114b.B21_G07 ESG0115b.B21_A06 ESG0115d.B21_E01 HL01369 LP01114 LP01155 LP01189 LP01228 LP01276 LP01362 LP01377 LP01473 LP01536 LP01674 LP01725 LP01761 LP01903 LP01908 LP01924 LP02008 LP02048 LP02072 LP02081 LP02183 LP02207 LP02247 LP02287 LP02342 LP02415 LP02616 LP02640 LP02653 LP02813 LP02986 LP03178 LP03232 LP03235 LP03359 LP03379 LP03470 LP03471 LP03535 LP03555 LP03783 LP03911 LP03952 LP03992 LP04039 LP04079 LP04084 LP04122 LP04683 LP04823 LP04887 LP04917 LP04967 LP04974 LP05007 LP05008 LP05039 LP05081 LP05109 LP05243 LP05251 LP05294 LP05327 LP05367 LP05517 LP05701 LP05828 LP05837 LP05944 LP06002 LP06036 LP06234 LP06282 LP06533 LP06559 LP06624 LP06760 LP06837 LP06928 LP06933 LP06934 LP06941 LP07126 LP07164 LP07169 LP07185 LP07187 LP07346 LP07443 LP07447 LP07575 LP07584 LP07976 LP08005 LP08185 LP08226 LP08287 LP08308 LP08367 LP08377 LP08601 LP08679 LP08703 LP08708 LP08762 LP08792 LP08802 LP08977 LP09045 LP09051 LP09090 LP09191 LP09238 LP09242 LP09352 LP09383 LP09414 LP09439 LP09536 LP09640 LP09665 LP09742 LP09832 LP09945 LP09970 LP09992 LP09995 LP10009 LP10148 LP10155 LP10176 LP10289 LP10474 LP10485 LP10503 LP10627 LP10659 LP10675 LP10688 LP10702 LP10843 LP10934 LP10975 LP11040 LP11322 LP11379 LP11409 LP11428 LP11429 LP11430 LP11559 LP11689 LP11893 LP11949 LP12204 LP12246 LP12325 LP12422 LP12424 LP12449 LP12519 LP12740 LP12748 LP12768 LP12869 LP13028 LP13195 LP13272 LP13748 LP13780 LP13795 LP13939 LP13977 LP14044 LP14488 LP14546 LP14726 LP14970 LP15010 LP15028 LP15185 LP15269 LP15538 LP16045 LP16256 LP16304 LP16694 LP17020 LP17228 LP17692 LP17707 LP17715 LP17842 LP17957 LP18010 LP18191 LP18276 LP18483 LP18844 LP18913 LP19141 LP19252 LP19301 LP19391 LP19539 LP19544 LP19642 LP19813 LP19849 LP19861 LP19883 LP20070 LP20178 LP20309 LP20375 LP20817 LP20909 LP21079 LP21329 LP21413 LP21482 LP21627 LP21731 LP21779 LP21892 LP22756 LP23258 LP23271 LP23288 LP23290 LP23293 LP23613 LP23912 LP23986 LP24067 LP24294 UUGC0408
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0002565 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 45326
Antibody Information
Other Information
Discoverer
	Akam, 1977.
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: Lsp2 CG6806 (Misra, 2000.4.14)
Additional comments	Annotation CG11538 on opposite strand (eliminated in release 5.13) represents incorrect CDS. (FlyBase Genome Annotators, 2008)
Other Comments
	P-element mediated germline transformation demonstrates a 230bp larval enhancer, which includes an ecdysone response element (EcRE), specifically targets Lsp2 gene activity to the larval fat body. Additional negative regulatory elements dictate both tissue-specificity and unique spatial restriction within the adult fat body. (Benes et al., 1996) Ecol\CAT and Ecol\lacZ reporter gene constructs carrying deletion mutants of the 5' flanking region transfected into Schneider cells confer transient ecdysone inducibility. A single functional ecdysone response element (EcRE) is localised at position -75 relative to the Lsp2 transcription initiation site. Structural features of this EcRE determine its ability to induce ecdysone responsiveness at a lower ligand concentration and may form the basis for differential hormone responsiveness within the fat body. (Antoniewski et al., 1995) Ecdysteroid-regulated gene. (Andres et al., 1993) Codes for Lsp2, a glycosylated polypeptide of 80,000 molecular weight (Brock and Roberts, 1980) and one of the major serum proteins of third instar larvae; a homohexamer of molecular weight 450,000 with subunit molecular weight of 78-83 x 103 daltons. (Lindsley and Zimm, 1992) Hydrazine is used to release oligosaccharides from a purified Lsp2 protein, sequence analysis is performed. (Williams et al., 1991)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_080077 RefSeq (Proteins) • NP_524816 DDBJ / EMBL / GenBank • AAF49958 AAM50865 AI259828 AY119005 CAA66371 X97770 Entrez Gene - A searchable database of RefSeq genes. • 45326 UniProtKB/Swiss-Prot • Q24388
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Hemocyanin/hexamerin middle domain (IPR000896) Hemocyanin, C-terminal (IPR005203) Hemocyanin, N-terminal (IPR005204) Uncharacterised domain, di-copper centre (IPR008922) Hemocyanin/hexamerin (IPR013788) Immunoglobulin E-set (IPR014756) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • LP03332
Linkouts
	BioGRID - A database of protein and genetic interactions • 69593 DroID - A comprehensive database of gene and protein interactions. • FBgn0002565 DRSC - Results from RNAi screens. • FBgn0002565 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0002565 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG11538-RA;CG6806-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0002565 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 45326 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 45326 modMine - Data generated by the modENCODE project. • FBgn0002565
Synonyms & Secondary IDs ( 16 )
Reported As
Symbol Synonym	C23 (Vierstraete et al., 2004) CG6806 (Vierstraete et al., 2004, Fulton et al., 2001, Fujikawa et al., 2009, Ellis and Carney, 2010, Shibata et al., 2010, Ellis and Carney, 2011, Keleman et al., 2009.8.5, Reumer et al., 2009) CG11538 (FlyBase Genome Annotators, 2008) DmeLSP2 (Burmester, 2001, Burmester et al., 1998) LHP   Lsp-2 (Dauwalder et al., 2005, Zapata et al., 2002, Mousseron-Grall et al., 1997, Massey et al., 1997, Gordadze and Benes, 1996, Benes et al., 1996, Antoniewski et al., 1995, Andres et al., 1993, Benes et al., 1990, Parisi et al., 2010) LSP-2 (Burmester, 2000.3.29, Lepesant, 1996.5.9, Burmester et al., 1999, Roberts et al., 1998, Maschat et al., 1990, Deutsch et al., 1989, Hoogwerf et al., 1988, Yedvobnick and Levine, 1982, Sato et al., 1982) LSP 2 (Riddiford, 1993) LSP2 (Hoogwerf et al., 1988, Shibata et al., 2010) Lsp2 (Beller et al., 2006, Blanco et al., 2010, Lazareva et al., 2007, Nelliot et al., 2006, Hennig et al., 2006, Xun et al., 2009, Bauer et al., 2010, Inaki et al., 2007, Liu et al., 2009, Fujikawa et al., 2009, Juhász et al., 2008, Fernández-Ayala et al., 2010, Ellis and Carney, 2010, Ellis and Carney, 2011, Reumer et al., 2009, Sieber and Thummel, 2009, Garcia et al., 2011, Dalton et al., 2010) Pt-1 (Choudhary et al., 1992, )
Name Synonym	larval-hemolymph-protein   Larval serum protein-2 (Antoniewski et al., 1995) Larval serum protein 2 (Xun et al., 2009, Zhang et al., 2011, Parisi et al., 2010, Reumer et al., 2009, Xun et al., 2008) larval serum protein 2 (Hennig et al., 2006, Robinson et al., 2010) Protein-1
Secondary FlyBase IDs
	FBgn0036251
References ( 90 )
Generate a list of	All references relating to this gene ( 90 )
List References by type	Research paper  ( 64 ) Supplementary material  ( 4 ) Review  ( 2 ) Personal communication to FlyBase  ( 9 ) Abstract  ( 2 ) FlyBase analysis  ( 2 ) DNA/RNA sequence record  ( 1 ) Book  ( 1 ) Protein sequence record  ( 1 ) Thesis  ( 1 ) Curated genome annotation  ( 2 )
Recent research papers ( 4 )
	Baycin-Hizal et al., 2011, J. Proteome Res. 10(6): 2777--2784 GlycoFly: A Database of Drosophila N-linked Glycoproteins Identified Using SPEG-MS Techniques. [FBrf0213834] Ellis and Carney, 2011, Genetics 187(1): 157--169 Socially-Responsive Gene Expression in Male Drosophila melanogaster Is Influenced by the Sex of the Interacting Partner. [FBrf0212780] Garcia et al., 2011, BMC Mol. Biol. 12: 32 Functional characterization of the sciarid BhC4-1 core promoter in transgenic Drosophila. [FBrf0214770] Zhang et al., 2011, J. Exp. Biol. 214(23): 4021--4029 Divergent transcriptomic responses to repeated and single cold exposures in Drosophila melanogaster. [FBrf0216629] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...