FlyBase Gene Report: Dmel\Rab32

General Information

Symbol

Dmel\Rab32

Species

D. melanogaster

Name

Rab32

Annotation Symbol

CG8024

Feature Type

protein_coding_gene

FlyBase ID

FBgn0002567

Gene Model Status

Current

Stock Availability

23 publicly available

Gene Snapshot

Rab32 (Rab32) encodes a small GTPase that contributes to vesicle trafficking regulation. It is involved in eye development, autophagy and lipid storage. [Date last reviewed: 2019-09-26]

Other Summaries

Alliance
Auto summary
Gene Group
Red Book
Interactive Fly

Also Known As

ltd, lightoid, Rab-RP1, DmRab32, RabRP1

Key Links

Genomic Location

Cytogenetic map

45B2-45B3

Sequence location

2R:9,177,864..9,186,342 [-]

Recombination map

2-60

Sequence

Get Decorated FASTA

Genomic Maps

GBrowse JBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

[Detailed GO annotations]

Gene Group (FlyBase)

RAB GTPASES

Protein Family (UniProt)

Protein Signatures (InterPro)

Molecular Function (GO)
[Detailed GO annotations]

Experimental Evidence

protein binding

Predictions / Assertions

GTP binding; GTPase activity

Summaries

Gene Group (FlyBase)

RAB GTPASES -: The Rab family are members of the Ras superfamily of small GTPases. Rabs regulate vesicle trafficking including cargo selection, vesicle budding, transport, docking and targeting. They localize to different intracellular compartments directed by specific isoprenylation of C-terminal motifs. (Adapted from PMID:15731001).

Phenotypic Description (Red Book; Lindsley and Zimm 1992)

ltd: lightoid

Eye color clear, light, translucent yellowish pink. Resembles lt but is lighter, darkens with age. Ocelli colorless; larval Malpighian tubes colorless. Deficient in ommochrome synthesis. Lack detectable levels of 3OH kynurenine despite normal levels of kynurenine hydroxylase activity; administration of 3 hydroxykynurenine without effect (Phillips, Simmons, and Dowman, 1970, Biochem. Genet. 4: 481-87; Sullivan, Kitos, and Sullivan, 1973, Genetics 75: 651-61). Exhibits reduced levels of phenoazinone synthetase, an enzyme involved in the condensation of 3OH kynurenine molecules to xanthommatin, as do other mutants deficient in ommochrome synthesis (Phillips, Forrest, and Kulkarni, 1973, Genetics 73: 45-56). Uptake of kynurenine, which is produced in the fat body, by the Malpighian tubes, where it is converted to 3-hydrokynurenine, is defective; similar defect seen in eye discs (Sullivan and Sullivan, 1975, Biochem. Genet. 13: 603-13). Sullivan and Sullivan postulate that ltd is a transport mutant that prevents the substrate kynurenine from reaching the sites of its conversion to ommochrome. Phenotypic interaction with other eye-color mutants examined by Rudy and Carvalier (1971, J. Hered. 62: 131-34) and by Silva and Mensua, 1985, DIS 61: 156); produces nearly white eyes when combined with either rb or g.

ltd37b

Eye color of newly hatched adult bright red like v, darkens to a color like pr in old flies. Ocelli colorless; larval Malpighian tubes colorless. Viability excellent. RK1.

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the GBrowse view of Dmel\Rab32 or the JBrowse view of Dmel\Rab32 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.49

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.57

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Rab32-RB

FBtr0089931

NM_080370

2771

686

Rab32-RC

FBtr0089934

NM_165659

2347

388

Rab32-RD

FBtr0089933

NM_165658

1448

222

Rab32-RE

FBtr0089932

NM_165660

2156

388

Rab32-RH

FBtr0339369

NM_001299309

1284

214

Rab32-RI

FBtr0346635

NM_165663

1811

389

Rab32-RJ

FBtr0346636

NM_001299308

1342

214

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

Rab32-PB

FBpp0088870

74.7

686

6.07

NP_525109

AAF58970

Rab32-PC

FBpp0088873

42.6

388

6.77

NP_724764

AAM68817

Rab32-PD

FBpp0088872

24.9

222

6.52

NP_724763

AAM68816

Rab32-PE

FBpp0088871

42.6

388

6.77

NP_724765

AAM68818

Rab32-PH

FBpp0308460

24.0

214

5.40

NP_001286238

AHN56036

Rab32-PI

FBpp0312215

42.8

389

4.40

NP_724768

AAG22295

Rab32-PJ

FBpp0312216

24.0

214

5.40

NP_001286237

AHN56035

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

388 aa isoforms: Rab32-PC, Rab32-PE

214 aa isoforms: Rab32-PH, Rab32-PJ

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Rab32 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Gene Ontology (20 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

protein binding

inferred from physical interaction with FLYBASE:ca; FB:FBgn0000247

(Ma et al., 2004)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

GTP binding

inferred from electronic annotation with InterPro:IPR001806, InterPro:IPR005225

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

GTPase activity

inferred from sequence or structural similarity

(Armstrong, 2000)

inferred from sequence or structural similarity with FLYBASE:Rab11; FB:FBgn0015790

(FlyBase Curators, 2008--)

inferred from biological aspect of ancestor with PANTHER:PTN001292747

(assigned by GO_Central )

(Gaudet et al., 2011)

Biological Process (9 terms)

Terms Based on Experimental Evidence (6 terms)

CV Term

Evidence

References

compound eye pigmentation

inferred from genetic interaction with FLYBASE:p; FB:FBgn0086679

(Falcon-Perez et al., 2007)

eye pigment granule organization

inferred from mutant phenotype

(Ma et al., 2004)

ommochrome biosynthetic process

inferred from mutant phenotype

(Tearle, 1991)

regulation of autophagy

inferred from mutant phenotype

(Wang et al., 2012)

regulation of lipid storage

inferred from mutant phenotype

(Wang et al., 2012)

inferred from genetic interaction with FLYBASE:bmm; FB:FBgn0036449

(Wang et al., 2012)

regulation of neuron death

inferred from mutant phenotype

(assigned by ParkinsonsUK-UCL )

(Macleod et al., 2013)

inferred from genetic interaction with UniProtKB:Q5S007

(assigned by ParkinsonsUK-UCL )

(Macleod et al., 2013)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

intracellular protein transport

inferred from biological aspect of ancestor with PANTHER:PTN001292747

(assigned by GO_Central )

(Gaudet et al., 2011)

Rab protein signal transduction

inferred from sequence or structural similarity with FLYBASE:Rab11; FB:FBgn0015790

(FlyBase Curators, 2008--)

inferred from biological aspect of ancestor with PANTHER:PTN001292747

(assigned by GO_Central )

(Gaudet et al., 2011)

vesicle-mediated transport

inferred from sequence or structural similarity with FLYBASE:Rab11; FB:FBgn0015790

(FlyBase Curators, 2008--)

Cellular Component (8 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

autophagosome

inferred from direct assay

(Wang et al., 2012)

lysosome

inferred from direct assay

(Wang et al., 2012)

recycling endosome

inferred from high throughput direct assay

(Chan et al., 2011)

synapse

inferred from high throughput direct assay

(Chan et al., 2011)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

melanosome

inferred from biological aspect of ancestor with PANTHER:PTN000636041

(assigned by GO_Central )

(Gaudet et al., 2011)

mitochondrion

inferred from biological aspect of ancestor with PANTHER:PTN000636119

(assigned by GO_Central )

(Gaudet et al., 2011)

trans-Golgi network

inferred from biological aspect of ancestor with PANTHER:PTN000636041

(assigned by GO_Central )

(Gaudet et al., 2011)

vesicle

inferred from sequence or structural similarity with FLYBASE:Rab11; FB:FBgn0015790

(FlyBase Curators, 2008--)

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

expression microarray

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

eye

(Xu et al., 2004)

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism

Comment: rapidly degraded

(Fisher et al., 2012)

embryonic stage 11 -- 12

Malpighian tubule primordium

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic Malpighian tubule

(Fisher et al., 2012)

embryonic/larval fat body

(Fisher et al., 2012)

ventral nerve cord

(Fisher et al., 2012)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

eye

(Xu et al., 2004)

Additional Descriptive Data

Isoform-specific expression patterns are observed. Sex-specific expression of alternative transcripts observed.

(Telonis-Scott et al., 2009)

Eye-enriched transcripts determined by ratio of expression level in wild-type heads. versus expression level in so heads.

(Xu et al., 2004)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

autophagosome

inferred from direct assay

(Wang et al., 2012)

lysosome

inferred from direct assay

(Wang et al., 2012)

recycling endosome

inferred from high throughput direct assay

(Chan et al., 2011)

synapse

inferred from high throughput direct assay

(Chan et al., 2011)

Expression Deduced from Reporters

Reporter: PBac{I-SceI(FRT.Rab32-GAL4.ex1(loxP.3xP3-RFP))}

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

neuron | subset of embryonic/larval brain

(Chan et al., 2011)

glial cell | subset of embryonic/larval brain

(Chan et al., 2011)

neuron | subset of ventral nerve cord

(Chan et al., 2011)

Bolwig organ | restricted

(Jin et al., 2012)

ventral thoracic disc | restricted

(Jin et al., 2012)

pupal stage P6 -- P7

neuron | subset of pupal brain

Comment: 30-40h APF

(Chan et al., 2011)

adult stage

neuron | subset of adult brain

(Chan et al., 2011)

neuron of adult optic lobe | faint

(Jin et al., 2012)

adult insulin secreting cell of pars intercerebralis

(Jin et al., 2012)

neuron of lamina

(Jin et al., 2012)

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Rab32 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Rab32

View exonic expression by tissue for Dmel\Rab32

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0002567

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0002567

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG8024-RC

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG8024

Flygut - An atlas of the Drosophila adult midgut

FBgn0002567

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\Rab32

Stages(s) 4-6
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, and Transgenic Constructs

Classical and Insertion Alleles ( 15 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 10 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Rab32

Transgenic constructs containing regulatory region of Rab32

Deletions and Duplications ( 30 )

Not disrupted in

Df(2R)cn1b

(Yannopoulos et al., 1981)

Df(2R)cn1

(Yannopoulos et al., 1981)

Df(2R)cn2b

(Yannopoulos et al., 1981)

Df(2R)cn2

(Yannopoulos et al., 1981)

Df(2R)cn3

(Yannopoulos et al., 1981)

Df(2R)cn4

(Yannopoulos et al., 1981)

Df(2R)cn5

(Yannopoulos et al., 1981)

Df(2R)cn6

(Yannopoulos et al., 1981)

Df(2R)cn7

(Yannopoulos et al., 1981)

Df(2R)cn8

(Yannopoulos et al., 1981)

Df(2R)cn9

(Yannopoulos et al., 1981)

Df(2R)cn10

(Yannopoulos et al., 1981)

Df(2R)cn11

(Yannopoulos et al., 1981)

Df(2R)cn12

(Yannopoulos et al., 1981)

Df(2R)cn13

(Yannopoulos et al., 1981)

Df(2R)cn14

(Yannopoulos et al., 1981)

Df(2R)cn15

(Yannopoulos et al., 1981)

Df(2R)cn16

(Yannopoulos et al., 1981)

Df(2R)cn17

(Yannopoulos et al., 1981)

Df(2R)cn18

(Yannopoulos et al., 1981)

Df(2R)cn19

(Yannopoulos et al., 1981)

Df(2R)cn20

(Yannopoulos et al., 1981)

Df(2R)cn21

(Yannopoulos et al., 1981)

Dp(2;2)Y3b

(Yannopoulos et al., 1981)

Dp(3;3)Y13

(Yannopoulos et al., 1981)

In(2R)cn10

(Yannopoulos et al., 1981)

In(2R)cn13

(Yannopoulos et al., 1981)

T(Y;2)J-D

Tp(2;Y)C

Not duplicated in

Dp(2;3)P32

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable

Sterility

Allele

fertile

Rab32^EY07166

Other Phenotypes

Allele

eye color defective

memory defective

neuroanatomy defective | adult stage

Rab32^unspecified

small body | larval stage, with Scer\GAL4^grh.D4

visible | recessive

Rab32^unspecified

Phenotype manifest in

Allele

dopaminergic PPL1 neuron

Rab32^unspecified

dopaminergic PPM1 neuron

Rab32^unspecified

eye photoreceptor cell & pigment granule

Rab32¹

Malpighian tubule

ocellus pigment granule

pigment cell

primary pigment cell pigment granule

Rab32¹

secondary pigment cell pigment granule

Rab32¹

tertiary pigment cell pigment granule

Rab32¹

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v7.1)

Homo sapiens (Human) (4)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\RAB32

10 of 15

Yes

Hsap\RAB38

6 of 15

Yes

Hsap\RAB29

2 of 15

Yes

Hsap\MRAS

1 of 15

Yes

Model Organism Orthologs (via DIOPT v7.1)

Mus musculus (laboratory mouse) (5)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Rab32

9 of 15

Yes

Mmus\Rab38

6 of 15

Yes

Mmus\Rab29

2 of 15

Yes

Mmus\Mras

1 of 15

Yes

Mmus\Rasef

1 of 15

Yes

Rattus norvegicus (Norway rat) (4)

Rnor\Rab32

9 of 13

Yes

Rnor\Rab38

5 of 13

Yes

Rnor\Rab29

2 of 13

Yes

Rnor\Rasef

1 of 13

Yes

Xenopus tropicalis (Western clawed frog) (3)

Xtro\rab32

7 of 12

Yes

Xtro\rab38

5 of 12

Yes

Xtro\LOC100496061

3 of 12

Yes

Danio rerio (Zebrafish) (6)

Drer\rab32a

8 of 15

Yes

Drer\rab38c

5 of 15

Yes

Drer\zgc:162171

5 of 15

Yes

Drer\rab32b

4 of 15

Yes

Drer\rab38b

4 of 15

Yes

Drer\cracr2ab

1 of 15

Yes

Caenorhabditis elegans (Nematode, roundworm) (4)

Cele\glo-1

8 of 15

Yes

Cele\nhr-207

1 of 15

Cele\R07B1.8

1 of 15

Yes

Cele\ras-2

1 of 15

Yes

Arabidopsis thaliana (thale-cress) (0)

No records found.

Saccharomyces cerevisiae (Brewer's yeast) (1)

Scer\YPT7

1 of 15

Yes

Schizosaccharomyces pombe (Fission yeast) (0)

No records found.

Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG091906IW )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Dmel\Rab32

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA20772

Drosophila persimilis

Dper\GL17459

Drosophila willistoni

Dwil\GK22864

Drosophila virilis

Dvir\GJ18542

Drosophila mojavensis

Dmoj\GI19816

Drosophila grimshawi

Dgri\GH19866

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG0915065Z )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17015225

Glossina morsitans

Tsetse fly

Gmor\GMOY003721

Mayetiola destructor

Hessian fly

Mdes\Mdes006483

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL006880

Anopheles darlingi

American malaria mosquito

Adar\ADAC004728

Anopheles gambiae

Malaria mosquito

Agam\AGAP007654

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ012804

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W067B )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA002711

Danaus plexippus

Monarch butterfly

Dple\DPOGS201533-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL009083

Apis florea

Little honeybee

Aflo\2730558900

Apis mellifera

Western honey bee

Amel\GB51798

Apis mellifera

Western honey bee

Amel\GB53276

Apis mellifera

Western honey bee

Amel\GB53277

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP19442

Bombus terrestris

Buff-tailed bumblebee

Bter\15211111

Linepithema humile

Argentine ant

Lhum\LH12107

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:002cc0

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG018795

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG030018

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG037341

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG020244

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_02502

Tribolium castaneum

Red flour beetle

Tcas\TC014165

Pediculus humanus

Human body louse

Phum\PHUM086930

Rhodnius prolixus

Kissing bug

Rpro\RPRC009223

Rhodnius prolixus

Kissing bug

Rpro\RPRC001576

Cimex lectularius

Bed bug

Clec\CLEC004656

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI002292

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_02473

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X062T )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR010476

Ixodes scapularis

Black-legged tick

Isca\ISCW001188

Stegodyphus mimosarum

African social velvet spider

Smim\KFM81211.1

Stegodyphus mimosarum

African social velvet spider

Smim\KFM81902.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur26g00740

Tetranychus urticae

Two-spotted spider mite

Turt\tetur15g01600

Tetranychus urticae

Two-spotted spider mite

Turt\tetur19g00930

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_39774

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_305892

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0K53 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Rab7l1

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_018305gn

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Rab32

Ciona intestinalis

Vase tunicate

Cint\LOC100178266

Gallus gallus

Domestic chicken

Ggal\ENSGALG00000028018

Gallus gallus

Domestic chicken

Ggal\RAB32

Gallus gallus

Domestic chicken

Ggal\RAB7L1

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v7.1)

Drosophila melanogaster (Fruit fly) (34)

Gene Symbol

Score

Source

Alignment

2 of 10

2 of 10

2 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Parkinson disease 16 (postulated), RAB29-related

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Allele

Disease

Evidence

References

Rab32^unspecified

model of Parkinson's disease

CEA

(Macleod et al., 2013)

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 3 )

Allele

Disease

Interaction

References

Rab32¹

exacerbates Hermansky-Pudlak syndrome

modeled by Blos1^ex2

(Cheli et al., 2010)

Rab32^{T33N.UASp.YFP}

ameliorates epilepsy

modeled by para^bss1

(Kroll et al., 2015)

Rab32^{Q79L.UASp.YFP}

ameliorates Parkinson's disease

modeled by Hsap\LRRK2^{G2019S.UAS.cMa}

(Macleod et al., 2013)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

RAB32; RAB32, member RAS oncogene family

10 of 15

612906

RAB38; RAB38, member RAS oncogene family

6 of 15

606281

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

RNA-RNA

Physical Interaction

Assay

References

Rab32 - mir-184

luminiscence technology

(Iovino et al., 2009)

protein-protein

Physical Interaction

Assay

References

Rab32 - didum

pull down, anti tag western blot, anti tag coimmunoprecipitation, autoradiography, two hybrid

(Ji et al., 2015, Satoh et al., 2008)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Rab32

enhanceable

aux

(Cheli et al., 2010)

Rab32

enhanceable

Blos1

(Cheli et al., 2010)

Rab32

enhanceable

(Lloyd et al., 2002)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

bchs

enhanceable

Rab32

(Simonsen et al., 2007)

Blos1

enhanceable

Rab32

(Cheli et al., 2010)

enhanceable

Rab32

(Falcon-Perez et al., 2007)

para

suppressible

Rab32

(Kroll et al., 2015)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

61778

DroID - A comprehensive database of gene and protein interactions.

FBgn0002567

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

35940

MIST (protein-protein) - An integrated Molecular Interaction Database

Rab32

Pathways

Gene Group - Pathway Membership (FlyBase)

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-60

Cytogenetic map

45B2-45B3

Sequence location

2R:9,177,864..9,186,342 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

45B2-45B3

Limits computationally determined from genome sequence between P{lacW}CG8788^k04512&P{lacW}l(2)k13412^k13412 and P{PZ}Pkn⁰⁶⁷³⁶

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

45A8-45B5

(Ozaki, 1999.12.8)

Experimentally Determined Recombination Data

Location

2-60

(FlyBase, 2016)

2-58.2

(Comeron et al., 2012)

2-[59]

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (23)

Bloomington

338

Rab32¹

429

ptc^tuf-1 Rab32¹

62558

w¹¹¹⁸; TI{TI}Rab32^EYFP

24448

w¹¹¹⁸; Mi{ET1}Rab32^MB03690

d06912

e00716

e02183

105856

105908

125815

w¹¹¹⁸; P{RS5}Rab32^5-HA-5064

VDRC

v330262

P{VSH330262}attP40

v104348

P{KK107695}VIE-260B

More stocks available...

Genomic Clones (17)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (140)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequences

BDGP DGC clones

Other clones

FI09421

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0002567

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0002567

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

35940

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: Rab32 ltd

(FlyBase, 2009-)

Source for database merge of

Source for merge of: ltd Rab-RP1

(Ma et al., 2004)

Source for merge of: ltd anon-WO0118547.106

(FlyBase, 1996-)

Additional comments

Renamed from 'ltd' to 'Rab32' (a popular synonym) to be consistent with the nomenclature of all other Rab genes.

(FlyBase, 2009-)

Source for merge of ltd anon-WO0118547.106 was sequence comparison ( date:051113 ).

(FlyBase, 1996-)

Other Comments

dsRNA has been made from templates generated with primers directed against this gene.

(Kouranti et al., 2006)

Shows particularly robust cycling of transcription in adult heads, as assessed by expression analysis using high density oligonucleotide arrays with probe generated during three 12-point time course experiments over the course of 6 days. Shows significant change of expression pattern in circadian mutant background; decreased expression in per⁰¹, tim⁰¹ and Clk^Jrk background.

(Claridge-Chang et al., 2001)

Area matching Drosophila EST AA979014.

(Davies, 2001.3.15)

Used as a marker gene, results of LUG and SIP analysis shows genes conferring resistance to parasitism by A.tabida are restricted to the second chromosome.

(Orr and Irving, 1997)

ltd is required for the synthesis of ommochrome in any tissue and may be a component of the ommochrome precursor transport system.

(Tearle, 1991)

Lesions in ltd reduce or eliminate pigmentation in the eyes and ocelli and block pigmentation of the fat body and tubules.

(Tearle, 1991)

Pteridines (neodrosopterin, drosopterin, isodrosopterin, aurodrosopterin, 6-acetyl-dihydrohomopterin, sepiapterin, dihydrobiopterin, biopterin, pterin and isoxanthopterin) have been quantified in single and double mutants.

(Silva and Mensua, 1985)

It is likely that this gene is required for the normal transport of eye-pigment precursors.

(Silva and Mensua, 1985)

Origin and Etymology

Discoverer

Nichols-Skoog, 6th April 1936.

Etymology

Given the name "beck", after one of Pavlov's dogs.

(Dubnau et al., 2003)

Identification

External Crossreferences and Linkouts ( 75 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

35940

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0002567

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0002567

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG8024

Flygut - An atlas of the Drosophila adult midgut

FBgn0002567

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

35940

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG8024

modMine - A data warehouse for the modENCODE project

FBgn0002567

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Linkouts

BioGRID - A database of protein and genetic interactions.

61778

DroID - A comprehensive database of gene and protein interactions.

FBgn0002567

DRSC - Results frm RNAi screens

FBgn0002567

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0002567

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG8024-RC

FlyMine - An integrated database for Drosophila genomics

FBgn0002567

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

35940

MIST (protein-protein) - An integrated Molecular Interaction Database

Rab32

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Synonyms and Secondary IDs (24)

Reported As

Symbol Synonym

24652026

(Rojas et al., 2012)

24652028

(Rojas et al., 2012)

A1Z7S1

(Rojas et al., 2012)

CG8024

(Meadows, 2017.1.11, Dunst et al., 2015, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Perkins et al., 2009, Goldman and Arbeitman, 2007, Muse et al., 2007, Zhang et al., 2007, Colicelli, 2004, Ma et al., 2004, Dubnau et al., 2003, Giot et al., 2003, Mount, 2001.8.14)

DRABR1

(Kohno et al., 1994)

DmRab32

(Pereira-Leal and Seabra, 2001, Pereira-Leal and Seabra, 2001)

DrabRP1

(Satoh et al., 1997)

Ltd

(Ji et al., 2015)

RAB-RP1

(Ganguly-Fitzgerald et al., 2006)

RAB7L

(Xiong and Yu, 2018)

Rab-RP1

(Berger et al., 2008, Ma et al., 2004, Claridge-Chang et al., 2001)

Rab-RP1/CG8024

(Chang et al., 2004)

Rab-r1

Rab32

(Krämer et al., 2019, Coutinho-Budd et al., 2017, Caviglia et al., 2016, Kroll et al., 2015, Rodriguez-Fernandez and Dell'Angelica, 2015, White et al., 2015, Gillingham et al., 2014, Gillingham et al., 2014, Wang et al., 2012, Iovino et al., 2009, Zhang et al., 2007, Zhang et al., 2007, Kouranti et al., 2006, Armstrong, 2000)

Rab32/RP1

(Zhang et al., 2007)

Rab7L1

(Macleod et al., 2013)

RabRP1

(Xu et al., 2004, Fujikawa et al., 2003)

anon-WO0118547.106

beck

(Berger et al., 2008)

ltd

(Rodriguez-Fernandez and Dell'Angelica, 2015, Ugrankar et al., 2015, Gupta et al., 2014, Japanese National Institute of Genetics, 2012.5.21, Wang et al., 2012, Harris et al., 2011, Napoletano et al., 2011, Cheli et al., 2010, Cheli et al., 2010, Gan et al., 2010, Rodriguez-Fernandez and Dell'angelica, 2009, Telonis-Scott et al., 2009, Satoh et al., 2008, Bakal et al., 2007, Falcon-Perez et al., 2007, Muse et al., 2007, Cherry and Perrimon, 2004)

rab32

(Lammers et al., 2017, Jin et al., 2012, Chan et al., 2011)

Name Synonyms

Lightoid

(Cheli et al., 2010, Satoh et al., 2008)

Rab-related protein 1

Rab32

lightoid

(Macleod et al., 2013, Pandey et al., 2013, Wang et al., 2012, Harris et al., 2011, Yoshimura et al., 2010, Al-Anzi et al., 2009, Rodriguez-Fernandez and Dell'angelica, 2009, Berger et al., 2008, Falcon-Perez et al., 2007, Goldman and Arbeitman, 2007, Simonsen et al., 2007, Cherry and Perrimon, 2004)

Secondary FlyBase IDs

FBgn0015788
FBgn0062240

Datasets (1)

Study focus (1)

Experimental Role

Project

Project Type

Title

bait_protein

Rab_Effectors_interaction_map

interactome

Interaction map generated by purification of most Rab factors, with identification of copurifying effectors by mass spectrometry.

References (117)

Report Sections

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