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General Information
Symbol
Dmel\Rab32
Species
D. melanogaster
Name
Rab32
Annotation Symbol
CG8024
Feature Type
FlyBase ID
FBgn0002567
Gene Model Status
Stock Availability
Gene Snapshot
Rab32 (Rab32) encodes a small GTPase that contributes to vesicle trafficking regulation. It is involved in eye development, autophagy and lipid storage. [Date last reviewed: 2019-09-26]
Also Known As
ltd, lightoid, Rab-RP1, DmRab32, RabRP1
Key Links
Genomic Location
Cytogenetic map
Sequence location
2R:9,177,864..9,186,342 [-]
Recombination map
2-60
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Gene Group (FlyBase)
Protein Family (UniProt)
-
Molecular Function (GO)
[Detailed GO annotations]
Experimental Evidence
Predictions / Assertions
Summaries
Gene Group (FlyBase)
RAB GTPASES -
The Rab family are members of the Ras superfamily of small GTPases. Rabs regulate vesicle trafficking including cargo selection, vesicle budding, transport, docking and targeting. They localize to different intracellular compartments directed by specific isoprenylation of C-terminal motifs. (Adapted from PMID:15731001).
Phenotypic Description (Red Book; Lindsley and Zimm 1992)
ltd: lightoid
Eye color clear, light, translucent yellowish pink. Resembles lt but is lighter, darkens with age. Ocelli colorless; larval Malpighian tubes colorless. Deficient in ommochrome synthesis. Lack detectable levels of 3OH kynurenine despite normal levels of kynurenine hydroxylase activity; administration of 3 hydroxykynurenine without effect (Phillips, Simmons, and Dowman, 1970, Biochem. Genet. 4: 481-87; Sullivan, Kitos, and Sullivan, 1973, Genetics 75: 651-61). Exhibits reduced levels of phenoazinone synthetase, an enzyme involved in the condensation of 3OH kynurenine molecules to xanthommatin, as do other mutants deficient in ommochrome synthesis (Phillips, Forrest, and Kulkarni, 1973, Genetics 73: 45-56). Uptake of kynurenine, which is produced in the fat body, by the Malpighian tubes, where it is converted to 3-hydrokynurenine, is defective; similar defect seen in eye discs (Sullivan and Sullivan, 1975, Biochem. Genet. 13: 603-13). Sullivan and Sullivan postulate that ltd is a transport mutant that prevents the substrate kynurenine from reaching the sites of its conversion to ommochrome. Phenotypic interaction with other eye-color mutants examined by Rudy and Carvalier (1971, J. Hered. 62: 131-34) and by Silva and Mensua, 1985, DIS 61: 156); produces nearly white eyes when combined with either rb or g.
ltd37b
Eye color of newly hatched adult bright red like v, darkens to a color like pr in old flies. Ocelli colorless; larval Malpighian tubes colorless. Viability excellent. RK1.
Gene Model and Products
Number of Transcripts
7
Number of Unique Polypeptides
5

Please see the GBrowse view of Dmel\Rab32 or the JBrowse view of Dmel\Rab32 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model
Gene model reviewed during 5.49
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.57
Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0089931
2771
686
FBtr0089934
2347
388
FBtr0089933
1448
222
FBtr0089932
2156
388
FBtr0339369
1284
214
FBtr0346635
1811
389
FBtr0346636
1342
214
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0088870
74.7
686
6.07
FBpp0088873
42.6
388
6.77
FBpp0088872
24.9
222
6.52
FBpp0088871
42.6
388
6.77
FBpp0308460
24.0
214
5.40
FBpp0312215
42.8
389
4.40
FBpp0312216
24.0
214
5.40
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

388 aa isoforms: Rab32-PC, Rab32-PE
214 aa isoforms: Rab32-PH, Rab32-PJ
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Rab32 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Gene Ontology (20 terms)
Molecular Function (3 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
inferred from physical interaction with FLYBASE:ca; FB:FBgn0000247
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
inferred from electronic annotation with InterPro:IPR001806, InterPro:IPR005225
(assigned by InterPro )
inferred from sequence or structural similarity
inferred from sequence or structural similarity with FLYBASE:Rab11; FB:FBgn0015790
inferred from biological aspect of ancestor with PANTHER:PTN001292747
(assigned by GO_Central )
Biological Process (9 terms)
Terms Based on Experimental Evidence (6 terms)
CV Term
Evidence
References
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from genetic interaction with FLYBASE:bmm; FB:FBgn0036449
inferred from mutant phenotype
(assigned by ParkinsonsUK-UCL )
inferred from genetic interaction with UniProtKB:Q5S007
(assigned by ParkinsonsUK-UCL )
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN001292747
(assigned by GO_Central )
inferred from sequence or structural similarity with FLYBASE:Rab11; FB:FBgn0015790
inferred from biological aspect of ancestor with PANTHER:PTN001292747
(assigned by GO_Central )
inferred from sequence or structural similarity with FLYBASE:Rab11; FB:FBgn0015790
Cellular Component (8 terms)
Terms Based on Experimental Evidence (4 terms)
CV Term
Evidence
References
inferred from direct assay
inferred from direct assay
inferred from high throughput direct assay
inferred from high throughput direct assay
Terms Based on Predictions or Assertions (4 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000636041
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000636119
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000636041
(assigned by GO_Central )
inferred from sequence or structural similarity with FLYBASE:Rab11; FB:FBgn0015790
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
expression microarray
Stage
Tissue/Position (including subcellular localization)
Reference
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
organism

Comment: maternally deposited

organism

Comment: rapidly degraded

northern blot
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
Isoform-specific expression patterns are observed. Sex-specific expression of alternative transcripts observed.
Eye-enriched transcripts determined by ratio of expression level in wild-type heads. versus expression level in so heads.
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
inferred from direct assay
inferred from direct assay
inferred from high throughput direct assay
inferred from high throughput direct assay
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Rab32 in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 4-6
  • Stages(s) 11-12
  • Stages(s) 13-16
Alleles, Insertions, and Transgenic Constructs
Phenotypes
Orthologs
Human Orthologs (via DIOPT v7.1)
Homo sapiens (Human) (4)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
10 of 15
Yes
Yes
 
6 of 15
No
Yes
2 of 15
No
Yes
1 of 15
No
Yes
Model Organism Orthologs (via DIOPT v7.1)
Mus musculus (laboratory mouse) (5)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
9 of 15
Yes
Yes
6 of 15
No
Yes
2 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
Rattus norvegicus (Norway rat) (4)
9 of 13
Yes
Yes
5 of 13
No
Yes
2 of 13
No
Yes
1 of 13
No
Yes
Xenopus tropicalis (Western clawed frog) (3)
7 of 12
Yes
Yes
5 of 12
No
Yes
3 of 12
No
Yes
Danio rerio (Zebrafish) (6)
8 of 15
Yes
Yes
5 of 15
No
Yes
5 of 15
No
Yes
4 of 15
No
Yes
4 of 15
No
Yes
1 of 15
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (4)
8 of 15
Yes
Yes
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
Yes
Arabidopsis thaliana (thale-cress) (0)
No records found.
Saccharomyces cerevisiae (Brewer's yeast) (1)
1 of 15
Yes
No
Schizosaccharomyces pombe (Fission yeast) (0)
No records found.
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG091906IW )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila melanogaster
fruit fly
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila sechellia
Drosophila erecta
Drosophila yakuba
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila persimilis
Drosophila willistoni
Drosophila virilis
Drosophila mojavensis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG0915065Z )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Glossina morsitans
Tsetse fly
Mayetiola destructor
Hessian fly
Aedes aegypti
Yellow fever mosquito
Anopheles darlingi
American malaria mosquito
Anopheles gambiae
Malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W067B )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Danaus plexippus
Monarch butterfly
Heliconius melpomene
Postman butterfly
Apis florea
Little honeybee
Apis mellifera
Western honey bee
Apis mellifera
Western honey bee
Apis mellifera
Western honey bee
Bombus impatiens
Common eastern bumble bee
Bombus terrestris
Buff-tailed bumblebee
Linepithema humile
Argentine ant
Megachile rotundata
Alfalfa leafcutting bee
Nasonia vitripennis
Parasitic wasp
Nasonia vitripennis
Parasitic wasp
Nasonia vitripennis
Parasitic wasp
Nasonia vitripennis
Parasitic wasp
Dendroctonus ponderosae
Mountain pine beetle
Tribolium castaneum
Red flour beetle
Pediculus humanus
Human body louse
Rhodnius prolixus
Kissing bug
Rhodnius prolixus
Kissing bug
Cimex lectularius
Bed bug
Acyrthosiphon pisum
Pea aphid
Zootermopsis nevadensis
Nevada dampwood termite
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X062T )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Ixodes scapularis
Black-legged tick
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Tetranychus urticae
Two-spotted spider mite
Tetranychus urticae
Two-spotted spider mite
Tetranychus urticae
Two-spotted spider mite
Daphnia pulex
Water flea
Daphnia pulex
Water flea
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0K53 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Ciona intestinalis
Vase tunicate
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Paralogs
Paralogs (via DIOPT v7.1)
Drosophila melanogaster (Fruit fly) (34)
2 of 10
2 of 10
2 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
Disease Model Summary Ribbon
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Allele
Disease
Evidence
References
Potential Models Based on Orthology ( 0 )
Human Ortholog
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 3 )
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)
Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
Homo sapiens (Human)
Gene name
Score
OMIM
OMIM Phenotype
DO term
Complementation?
Transgene?
Functional Complementation Data
Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
Interactions
Summary of Physical Interactions
esyN Network Diagram
Show neighbor-neighbor interactions:
Select Layout:
Legend:
Protein
RNA
Selected Interactor(s)
Interactions Browser

Please see the Physical Interaction reports below for full details
RNA-RNA
Physical Interaction
Assay
References
protein-protein
Physical Interaction
Assay
References
Summary of Genetic Interactions
esyN Network Diagram
esyN Network Key:
Suppression
Enhancement

Please look at the allele data for full details of the genetic interactions
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
External Data
Linkouts
BioGRID - A database of protein and genetic interactions.
DroID - A comprehensive database of gene and protein interactions.
InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
MIST (protein-protein) - An integrated Molecular Interaction Database
Pathways
Gene Group - Pathway Membership (FlyBase)
External Data
Linkouts
Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
Genomic Location and Detailed Mapping Data
Chromosome (arm)
2R
Recombination map
2-60
Cytogenetic map
Sequence location
2R:9,177,864..9,186,342 [-]
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
45B2-45B3
Limits computationally determined from genome sequence between P{lacW}CG8788k04512&P{lacW}l(2)k13412k13412 and P{PZ}Pkn06736
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
45A8-45B5
Experimentally Determined Recombination Data
Location
2-[59]
Left of (cM)
Right of (cM)
Notes
Stocks and Reagents
Stocks (23)
Genomic Clones (17)
 

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (140)
 

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequences
BDGP DGC clones
Other clones
Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

cDNA Clones, End Sequenced (ESTs)
RNAi and Array Information
Linkouts
DRSC - Results frm RNAi screens
GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
Antibody Information
Laboratory Generated Antibodies
 
Commercially Available Antibodies
 
Other Information
Relationship to Other Genes
Source for database identify of
Source for identity of: Rab32 ltd
Source for database merge of
Source for merge of: ltd Rab-RP1
Source for merge of: ltd anon-WO0118547.106
Additional comments
Renamed from 'ltd' to 'Rab32' (a popular synonym) to be consistent with the nomenclature of all other Rab genes.
Source for merge of ltd anon-WO0118547.106 was sequence comparison ( date:051113 ).
Other Comments
dsRNA has been made from templates generated with primers directed against this gene.
Shows particularly robust cycling of transcription in adult heads, as assessed by expression analysis using high density oligonucleotide arrays with probe generated during three 12-point time course experiments over the course of 6 days. Shows significant change of expression pattern in circadian mutant background; decreased expression in per01, tim01 and ClkJrk background.
Area matching Drosophila EST AA979014.
Used as a marker gene, results of LUG and SIP analysis shows genes conferring resistance to parasitism by A.tabida are restricted to the second chromosome.
ltd is required for the synthesis of ommochrome in any tissue and may be a component of the ommochrome precursor transport system.
Lesions in ltd reduce or eliminate pigmentation in the eyes and ocelli and block pigmentation of the fat body and tubules.
Pteridines (neodrosopterin, drosopterin, isodrosopterin, aurodrosopterin, 6-acetyl-dihydrohomopterin, sepiapterin, dihydrobiopterin, biopterin, pterin and isoxanthopterin) have been quantified in single and double mutants.
It is likely that this gene is required for the normal transport of eye-pigment precursors.
Origin and Etymology
Discoverer
Nichols-Skoog, 6th April 1936.
Etymology
Given the name "beck", after one of Pavlov's dogs.
Identification
External Crossreferences and Linkouts ( 75 )
Sequence Crossreferences
NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
Other crossreferences
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
KEGG Genes - Molecular building blocks of life in the genomic space.
modMine - A data warehouse for the modENCODE project
Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
Linkouts
BioGRID - A database of protein and genetic interactions.
DroID - A comprehensive database of gene and protein interactions.
DRSC - Results frm RNAi screens
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyMine - An integrated database for Drosophila genomics
InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
MIST (protein-protein) - An integrated Molecular Interaction Database
Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
Synonyms and Secondary IDs (24)
Reported As
Symbol Synonym
Rab-RP1/CG8024
Rab-r1
anon-WO0118547.106
Secondary FlyBase IDs
  • FBgn0015788
  • FBgn0062240
Datasets (1)
Study focus (1)
Experimental Role
Project
Project Type
Title
  • bait_protein
Interaction map generated by purification of most Rab factors, with identification of copurifying effectors by mass spectrometry.
References (117)