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Gene Dmel\E(spl)m3-HLH

General Information
Symbol	Dmel\E(spl)m3-HLH	Species	D. melanogaster
Name	Enhancer of split m3, helix-loop-helix	Annotation symbol	CG8346
Feature type	protein_coding_gene	FlyBase ID	FBgn0002609
Gene Model Status	Current	Stock availability	2 publicly available
Also Known As	m3, E(spl)m3, HLHM3, E(spl)C m3, HLH-m3, E(spl)C
Genomic Location
Chromosome (arm)	3R	Recombination map	3-89.1
Cytogenetic map	96F10-96F10	Sequence location	3R:21,847,545..21,848,849 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Enhancer of split m3, helix-loop-helix is referred to in FlyBase by the symbol Dmel\E(spl)m3-HLH (CG8346, FBgn0002609). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: sequence-specific DNA binding. It is reported to be involved in the biological process: Notch signaling pathway; compound eye development; negative regulation of transcription from RNA polymerase II promoter. 9 alleles are reported. The phenotype of these alleles is annotated with: scutellar bristle. It has one annotated transcript and one annotated polypeptide. Protein features are: Myc-type, basic helix-loop-helix (bHLH) domain; Orange; Orange subgroup. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of very low expression. Peak expression observed within 06-12 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system, adult midgut, larval trachea. Comments on Affy2 ProbeSet: ProbeSet 1623497_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of HLHm3. Gene sequence location is 3R:21847545..21848849.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 96F10-96F10   Limits computationally determined from genome sequence between P{PZ}l(3)rQ197rQ197 and P{lacW}scribj7B3
Experimentally Determined Cytological Location
Cytogenetic map Notes References 96F11-96F14     (Nellesen, 1998.12.18)
Experimentally Determined Recombination Data
Location	3-89.1
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\E(spl)m3-HLH for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.42 Gene model reviewed during 5.48
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) E(spl)m3-HLH-RA FBtr0084958  NM_079785   1305   224
Additional Transcript Data & Comments
Reported size (kB)	1.4 (northern blot) (Knust et al., 1987)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein E(spl)m3-HLH-PA   FBpp0084332   25.0   224   8.23     NP_524509   AAF56550
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Orange subgroup (IPR018352) Orange (IPR003650) Myc-type, basic helix-loop-helix (bHLH) domain (IPR011598)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE014297 AAF56550   AF115454   AY094858 AAM11211   AY779906 AAV59048   AY779907 AAV59060   AY779908 AAV59072   AY779909 AAV59084   AY779910 AAV59096   AY779911 AAV59108   AY779912 AAV59120   AY779913 AAV59132   AY779914 AAV59144   AY779915 AAV59156   AY779916 AAV59168   AY779917 AAV59180   AY779918 AAV59192   AY779919 AAV59204   AY779920 AAV59216   AY779921 AAV59228   AY906259 AAX60605   AY906260 AAX60606   AY906261 AAX60607   AY906262 AAX60608   AY906263 AAX60609   AY906264 AAX60610   AY906265 AAX60611   AY906266 AAX60612   AY906267 AAX60613   AY906268 AAX60614   AY906269 AAX60615   AY906270 AAX60616   AY906271 AAX60617   AY906272 AAX60618   AY906273 AAX60619   AY906274 AAX60620   AY906275 AAX60621   AY906276 AAX60622   AY906277 AAX60623   AY906278 AAX60624   AY906279 AAX60625   AY906280 AAX60626   AY906281 AAX60627   AY906282 AAX60628   AY906283 AAX60629   AY906284 AAX60630   AY906285 AAX60631   AY906286 AAX60632   AY906287 AAX60633   AY906288 AAX60634   AY906289 AAX60635   AY906290 AAX60636   AY906291 AAX60637   AY906292 AAX60638   AY906293 AAX60639   AY906294 AAX60640   AY906295 AAX60641   AY906296 AAX60642   AY906297 AAX60643   AY906298 AAX60644   AY906299 AAX60645   AY906300 AAX60646   AY906301 AAX60647   AY906302 AAX60648   AY906303 AAX60649   AY906304 AAX60650   AY906305 AAX60651   AY906306 AAX60652   AY906307 AAX60653   AY906308 AAX60654   AY906309 AAX60655   AY906310 AAX60656   AY906311 AAX60657   AY906312 AAX60658   AY906313 AAX60659   AY906314 AAX60660   AY906315 AAX60661   AY906316 AAX60662   AY906317 AAX60663   AY906318 AAX60664   AY906319 AAX60665   AY906320 AAX60666   AY906321 AAX60667   AY906322 AAX60668   AY906323 AAX60669   AY906324 AAX60670   AY906325 AAX60671   AY906326 AAX60672   AY906327 AAX60673   AY906328 AAX60674   AY906329 AAX60675   AY906330 AAX60676   AY906331 AAX60677   AY906332 AAX60678   AY906333 AAX60679   AY906334 AAX60680   AY906335 AAX60681   AY906336 AAX60682   AY906337 AAX60683   AY906338 AAX60684   AY906339 AAX60685   AY906340 AAX60686   AY906341 AAX60687   AY906342 AAX60688   AY906343 AAX60689   AY906344 AAX60690   AY906345 AAX60691   AY906346 AAX60692   AY906347 AAX60693   AY906348 AAX60694   AY906349 AAX60695   BI167978   BI213666   CO261931   CO263111   CO268629   CO268672   CO275920   CO291004   CO303109   CO303614   CO309644   CZ472012   EC059226   EC068476   EC070869   EC075926   EC082891   EC090547   EC201948   EC202745   EC203058   EC207900   EC214380   EC217037   EC217250   EC231206   EC242793   GH752898   GH756676   GH771091   GH772466   GH773137   GH793400   GH795152   GH795757   GH820444   GH835929   GH838876   GH870498   GH871054   GH879265   GH879289   GH879961   GH880374   GH884248   GH885115   GH886090   GH889269   GH904240   GH907982   GH915179   GH934016   GH937610   M96165 AAA28908   X67046 CAA47431
UniProtKB/Swiss-Prot	Q01068
UniProtKB/TrEMBL	Q56LC9 Q56LI3 Q56LA3 Q56LE8
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 8 -- 11   ventral neurectoderm   (Wech et al., 1999) proneural cluster   (Wech et al., 1999) embryonic stage 9   endoderm   (Wech et al., 1999) Comment:transiently expressed embryonic stage 9 -- 10   antennal primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium central brain primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium visual primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium dorsal head epidermis primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium lateral head epidermis primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium ventral head epidermis primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium ventral ectoderm   (Fisher et al., 2012) embryonic stage 11 -- 12   antennal primordium   (Fisher et al., 2012) Comment:reported as procephalon primordium central brain primordium   (Fisher et al., 2012) Comment:reported as procephalon primordium dorsal head epidermis primordium   (Fisher et al., 2012) Comment:reported as procephalon primordium lateral head epidermis primordium   (Fisher et al., 2012) Comment:reported as procephalon primordium ventral head epidermis primordium   (Fisher et al., 2012) Comment:reported as procephalon primordium visual primordium   (Fisher et al., 2012) Comment:reported as procephalon primordium anterior midgut primordium   (Fisher et al., 2012) atrium | precursor   (Fisher et al., 2012) Comment:reported as atrium primordium dorsal epidermis primordium   (Fisher et al., 2012) dorsal ridge primordium   (Fisher et al., 2012) head mesoderm   (Fisher et al., 2012) posterior midgut primordium   (Fisher et al., 2012) trunk mesoderm   (Fisher et al., 2012) ventral epidermis primordium   (Fisher et al., 2012) embryonic stage 12 -- 15   embryonic head segment   (Wech et al., 1999) embryonic thoracic segment   (Wech et al., 1999) embryonic stage 13 -- 16   embryonic dorsal epidermis   (Fisher et al., 2012) embryonic head epidermis   (Fisher et al., 2012) embryonic hindgut   (Fisher et al., 2012) embryonic ventral epidermis   (Fisher et al., 2012) embryonic/larval midgut   (Fisher et al., 2012) northern blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage 11 -- 16     (Knust et al., 1987) Comment:reference states 6-14 hr AEL embryonic stage 13   Malpighian tubule   (Knust et al., 1987) embryonic/larval midgut | restricted   (Knust et al., 1987) adult stage | female     (Knust et al., 1987)
Additional Descriptive Data
	The peak of Dsim\HLHm3 expression during embryogenesis occurs at 6-14 hours. Low levels of expression are also detected in adult females. By in situ hybridization, ubiquitous expression is detected throughout embryogenesis, with high levels in stage 11-15 embryos. At stage 13, expression is detected in unidentified structures in midgut walls, and in Malpighian tubules. (Knust et al., 1987)
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system, adult midgut, larval trachea. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    142.125 Larval Midgut    60.7 Larval Hindgut    21 Larval Malpighian Tubules    1.9 Larval Fat Body    17.1 Larval Salivary Gland    20.6 Larval Trachea    343.825 Larval Carcass    39.45 Adult Head    26.2 Adult Eye    28.425 Adult Brain    20.7 Adult Thoracic-Abdominal Ganglion    22.2 Adult Crop    6.4 Adult Midgut    163.6 Adult Hindgut    19.7 Adult Malpighian Tubules    6.2 Adult Fat Body    7.3 Adult Salivary Gland    7.7 Adult Heart    10.225 Adult VirginFemale Spermatheca    5.3 Adult InseminatedFemale Spermatheca    5.3 Adult Ovary    39.6 Adult Testis    5.8 Adult Male Accessory Gland    5.8 Adult Carcass    14.1 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    142.125 Larval Midgut    60.7 Larval Hindgut    21 Larval Malpighian Tubules    1.9 Larval Fat Body    17.1 Larval Salivary Gland    20.6 Larval Trachea    343.825 Larval Carcass    39.45 Adult Head    26.2 Adult Eye    28.425 Adult Brain    20.7 Adult Thoracic-Abdominal Ganglion    22.2 Adult Crop    6.4 Adult Midgut    163.6 Adult Hindgut    19.7 Adult Malpighian Tubules    6.2 Adult Fat Body    7.3 Adult Salivary Gland    7.7 Adult Heart    10.225 Adult VirginFemale Spermatheca    5.3 Adult InseminatedFemale Spermatheca    5.3 Adult Ovary    39.6 Adult Testis    5.8 Adult Male Accessory Gland    5.8 Adult Carcass    14.1 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    142.125 Larval Midgut    60.7 Larval Hindgut    21 Larval Malpighian Tubules    1.9 Larval Fat Body    17.1 Larval Salivary Gland    20.6 Larval Trachea    343.825 Larval Carcass    39.45 Adult Head    26.2 Adult Eye    28.425 Adult Brain    20.7 Adult Thoracic-Abdominal Ganglion    22.2 Adult Crop    6.4 Adult Midgut    163.6 Adult Hindgut    19.7 Adult Malpighian Tubules    6.2 Adult Fat Body    7.3 Adult Salivary Gland    7.7 Adult Heart    10.225 Adult VirginFemale Spermatheca    5.3 Adult InseminatedFemale Spermatheca    5.3 Adult Ovary    39.6 Adult Testis    5.8 Adult Male Accessory Gland    5.8 Adult Carcass    14.1 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    142.125 Larval Midgut    60.7 Larval Hindgut    21 Larval Malpighian Tubules    1.9 Larval Fat Body    17.1 Larval Salivary Gland    20.6 Larval Trachea    343.825 Larval Carcass    39.45 Adult Head    26.2 Adult Eye    28.425 Adult Brain    20.7 Adult Thoracic-Abdominal Ganglion    22.2 Adult Crop    6.4 Adult Midgut    163.6 Adult Hindgut    19.7 Adult Malpighian Tubules    6.2 Adult Fat Body    7.3 Adult Salivary Gland    7.7 Adult Heart    10.225 Adult VirginFemale Spermatheca    5.3 Adult InseminatedFemale Spermatheca    5.3 Adult Ovary    39.6 Adult Testis    5.8 Adult Male Accessory Gland    5.8 Adult Carcass    14.1 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    142.125 Larval Midgut    60.7 Larval Hindgut    21 Larval Malpighian Tubules    1.9 Larval Fat Body    17.1 Larval Salivary Gland    20.6 Larval Trachea    343.825 Larval Carcass    39.45 Adult Head    26.2 Adult Eye    28.425 Adult Brain    20.7 Adult Thoracic-Abdominal Ganglion    22.2 Adult Crop    6.4 Adult Midgut    163.6 Adult Hindgut    19.7 Adult Malpighian Tubules    6.2 Adult Fat Body    7.3 Adult Salivary Gland    7.7 Adult Heart    10.225 Adult VirginFemale Spermatheca    5.3 Adult InseminatedFemale Spermatheca    5.3 Adult Ovary    39.6 Adult Testis    5.8 Adult Male Accessory Gland    5.8 Adult Carcass    14.1 Expression Level Scale  None   Low   Moderate   High  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    142.125 Larval Midgut    60.7 Larval Hindgut    21 Larval Malpighian Tubules    1.9 Larval Fat Body    17.1 Larval Salivary Gland    20.6 Larval Trachea    343.825 Larval Carcass    39.45 Adult Head    26.2 Adult Eye    28.425 Adult Brain    20.7 Adult Thoracic-Abdominal Ganglion    22.2 Adult Crop    6.4 Adult Midgut    163.6 Adult Hindgut    19.7 Adult Malpighian Tubules    6.2 Adult Fat Body    7.3 Adult Salivary Gland    7.7 Adult Heart    10.225 Adult VirginFemale Spermatheca    5.3 Adult InseminatedFemale Spermatheca    5.3 Adult Ovary    39.6 Adult Testis    5.8 Adult Male Accessory Gland    5.8 Adult Carcass    14.1 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    142.125 Larval Midgut    60.7 Larval Hindgut    21 Larval Malpighian Tubules    1.9 Larval Fat Body    17.1 Larval Salivary Gland    20.6 Larval Trachea    343.825 Larval Carcass    39.45 Adult Head    26.2 Adult Eye    28.425 Adult Brain    20.7 Adult Thoracic-Abdominal Ganglion    22.2 Adult Crop    6.4 Adult Midgut    163.6 Adult Hindgut    19.7 Adult Malpighian Tubules    6.2 Adult Fat Body    7.3 Adult Salivary Gland    7.7 Adult Heart    10.225 Adult VirginFemale Spermatheca    5.3 Adult InseminatedFemale Spermatheca    5.3 Adult Ovary    39.6 Adult Testis    5.8 Adult Male Accessory Gland    5.8 Adult Carcass    14.1 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    142.125 Larval Midgut    60.7 Larval Hindgut    21 Larval Malpighian Tubules    1.9 Larval Fat Body    17.1 Larval Salivary Gland    20.6 Larval Trachea    343.825 Larval Carcass    39.45 Adult Head    26.2 Adult Eye    28.425 Adult Brain    20.7 Adult Thoracic-Abdominal Ganglion    22.2 Adult Crop    6.4 Adult Midgut    163.6 Adult Hindgut    19.7 Adult Malpighian Tubules    6.2 Adult Fat Body    7.3 Adult Salivary Gland    7.7 Adult Heart    10.225 Adult VirginFemale Spermatheca    5.3 Adult InseminatedFemale Spermatheca    5.3 Adult Ovary    39.6 Adult Testis    5.8 Adult Male Accessory Gland    5.8 Adult Carcass    14.1 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    26.2   NA  Eye    28.425   NA  Brain    20.7   142.125  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    22.2   NA  Crop    6.4   60.7  Midgut    163.6   21  Hindgut    no informative data   1.9  Malpighian Tubules    6.2   no informative data  Fat Body    7.3   20.6  Salivary Gland    7.7   NA  Heart    10.225   343.825  Trachea    NA   NA  VirginFemale Spermatheca    5.3   NA  InseminatedFemale Spermatheca    5.3   NA  Ovary    39.6   NA  Testis    5.8   NA  Male Accessory Gland    5.8   39.45  Carcass    no informative data FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    15 central nervous system, larvae L3    9 central nervous system, pupae P8    3 head, virgin 1-day female    1 head, virgin 4-day female    1 head, virgin 20-day female    0 head, mated 1-day female    3 head, mated 4-day female    1 head, mated 20-day female    0 head, mated 1-day male    2 head, mated 4-day male    1 head, mated 20-day male    1 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    2 digestive system, 4-day adult    5 digestive system, 20-day adult    7 fat body, larvae L3 wandering    0 fat body, white prepupae    3 fat body, pupae P8    0 carcass, larvae L3 wandering    13 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    0 ovary, virgin 4-day female    2 ovary, mated 4-day female    2 testis, mated 4-day male    1 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    15 central nervous system, larvae L3    9 central nervous system, pupae P8    3 head, virgin 1-day female    1 head, virgin 4-day female    1 head, virgin 20-day female    0 head, mated 1-day female    3 head, mated 4-day female    1 head, mated 20-day female    0 head, mated 1-day male    2 head, mated 4-day male    1 head, mated 20-day male    1 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    2 digestive system, 4-day adult    5 digestive system, 20-day adult    7 fat body, larvae L3 wandering    0 fat body, white prepupae    3 fat body, pupae P8    0 carcass, larvae L3 wandering    13 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    0 ovary, virgin 4-day female    2 ovary, mated 4-day female    2 testis, mated 4-day male    1 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    15 central nervous system, larvae L3    9 central nervous system, pupae P8    3 head, virgin 1-day female    1 head, virgin 4-day female    1 head, virgin 20-day female    0 head, mated 1-day female    3 head, mated 4-day female    1 head, mated 20-day female    0 head, mated 1-day male    2 head, mated 4-day male    1 head, mated 20-day male    1 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    2 digestive system, 4-day adult    5 digestive system, 20-day adult    7 fat body, larvae L3 wandering    0 fat body, white prepupae    3 fat body, pupae P8    0 carcass, larvae L3 wandering    13 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    0 ovary, virgin 4-day female    2 ovary, mated 4-day female    2 testis, mated 4-day male    1 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    15 central nervous system, larvae L3    9 central nervous system, pupae P8    3 head, virgin 1-day female    1 head, virgin 4-day female    1 head, virgin 20-day female    0 head, mated 1-day female    3 head, mated 4-day female    1 head, mated 20-day female    0 head, mated 1-day male    2 head, mated 4-day male    1 head, mated 20-day male    1 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    2 digestive system, 4-day adult    5 digestive system, 20-day adult    7 fat body, larvae L3 wandering    0 fat body, white prepupae    3 fat body, pupae P8    0 carcass, larvae L3 wandering    13 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    0 ovary, virgin 4-day female    2 ovary, mated 4-day female    2 testis, mated 4-day male    1 accessory gland, mated 4-day male    0 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    15 central nervous system, larvae L3    9 central nervous system, pupae P8    3 head, virgin 1-day female    1 head, virgin 4-day female    1 head, virgin 20-day female    0 head, mated 1-day female    3 head, mated 4-day female    1 head, mated 20-day female    0 head, mated 1-day male    2 head, mated 4-day male    1 head, mated 20-day male    1 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    2 digestive system, 4-day adult    5 digestive system, 20-day adult    7 fat body, larvae L3 wandering    0 fat body, white prepupae    3 fat body, pupae P8    0 carcass, larvae L3 wandering    13 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    0 ovary, virgin 4-day female    2 ovary, mated 4-day female    2 testis, mated 4-day male    1 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    15 central nervous system, larvae L3    9 central nervous system, pupae P8    3 head, virgin 1-day female    1 head, virgin 4-day female    1 head, virgin 20-day female    0 head, mated 1-day female    3 head, mated 4-day female    1 head, mated 20-day female    0 head, mated 1-day male    2 head, mated 4-day male    1 head, mated 20-day male    1 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    2 digestive system, 4-day adult    5 digestive system, 20-day adult    7 fat body, larvae L3 wandering    0 fat body, white prepupae    3 fat body, pupae P8    0 carcass, larvae L3 wandering    13 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    0 ovary, virgin 4-day female    2 ovary, mated 4-day female    2 testis, mated 4-day male    1 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    15 central nervous system, larvae L3    9 central nervous system, pupae P8    3 head, virgin 1-day female    1 head, virgin 4-day female    1 head, virgin 20-day female    0 head, mated 1-day female    3 head, mated 4-day female    1 head, mated 20-day female    0 head, mated 1-day male    2 head, mated 4-day male    1 head, mated 20-day male    1 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    2 digestive system, 4-day adult    5 digestive system, 20-day adult    7 fat body, larvae L3 wandering    0 fat body, white prepupae    3 fat body, pupae P8    0 carcass, larvae L3 wandering    13 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    0 ovary, virgin 4-day female    2 ovary, mated 4-day female    2 testis, mated 4-day male    1 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    15 central nervous system, larvae L3    9 central nervous system, pupae P8    3 head, virgin 1-day female    1 head, virgin 4-day female    1 head, virgin 20-day female    0 head, mated 1-day female    3 head, mated 4-day female    1 head, mated 20-day female    0 head, mated 1-day male    2 head, mated 4-day male    1 head, mated 20-day male    1 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    2 digestive system, 4-day adult    5 digestive system, 20-day adult    7 fat body, larvae L3 wandering    0 fat body, white prepupae    3 fat body, pupae P8    0 carcass, larvae L3 wandering    13 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    0 ovary, virgin 4-day female    2 ovary, mated 4-day female    2 testis, mated 4-day male    1 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of very low expression. Peak expression observed within 06-12 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    2 embryo 04-06hr    8 embryo 06-08hr    49 embryo 08-10hr    104 embryo 10-12hr    92 embryo 12-14hr    37 embryo 14-16hr    16 embryo 16-18hr    10 embryo 18-20hr    7 embryo 20-22hr    4 embryo 22-24hr    3 larva L1    5 larva L2    3 larva L3 12hr old    2 larva L3 puffstage 1-2    4 larva L3 puffstage 3-6    8 larva L3 puffstage 7-9    11 white prepupae new    12 white prepupae 12hr    17 white prepupae 24hr    19 pupae 2d postWPP    12 pupae 3d postWPP    7 pupae 4d postWPP    3 adult male 01day    2 adult male 05day    1 adult male 30day    1 adult female 01day    5 adult female 05day    2 adult female 30day    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    2 embryo 04-06hr    8 embryo 06-08hr  (49) embryo 08-10hr  (104) embryo 10-12hr  (92) embryo 12-14hr  (37) embryo 14-16hr    16 embryo 16-18hr    10 embryo 18-20hr    7 embryo 20-22hr    4 embryo 22-24hr    3 larva L1    5 larva L2    3 larva L3 12hr old    2 larva L3 puffstage 1-2    4 larva L3 puffstage 3-6    8 larva L3 puffstage 7-9    11 white prepupae new    12 white prepupae 12hr    17 white prepupae 24hr    19 pupae 2d postWPP    12 pupae 3d postWPP    7 pupae 4d postWPP    3 adult male 01day    2 adult male 05day    1 adult male 30day    1 adult female 01day    5 adult female 05day    2 adult female 30day    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    2 embryo 04-06hr    8 embryo 06-08hr    49 embryo 08-10hr  104 embryo 10-12hr    92 embryo 12-14hr    37 embryo 14-16hr    16 embryo 16-18hr    10 embryo 18-20hr    7 embryo 20-22hr    4 embryo 22-24hr    3 larva L1    5 larva L2    3 larva L3 12hr old    2 larva L3 puffstage 1-2    4 larva L3 puffstage 3-6    8 larva L3 puffstage 7-9    11 white prepupae new    12 white prepupae 12hr    17 white prepupae 24hr    19 pupae 2d postWPP    12 pupae 3d postWPP    7 pupae 4d postWPP    3 adult male 01day    2 adult male 05day    1 adult male 30day    1 adult female 01day    5 adult female 05day    2 adult female 30day    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    2 embryo 04-06hr    8 embryo 06-08hr    49 embryo 08-10hr    104 embryo 10-12hr    92 embryo 12-14hr    37 embryo 14-16hr    16 embryo 16-18hr    10 embryo 18-20hr    7 embryo 20-22hr    4 embryo 22-24hr    3 larva L1    5 larva L2    3 larva L3 12hr old    2 larva L3 puffstage 1-2    4 larva L3 puffstage 3-6    8 larva L3 puffstage 7-9    11 white prepupae new    12 white prepupae 12hr    17 white prepupae 24hr    19 pupae 2d postWPP    12 pupae 3d postWPP    7 pupae 4d postWPP    3 adult male 01day    2 adult male 05day    1 adult male 30day    1 adult female 01day    5 adult female 05day    2 adult female 30day    3 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    2 embryo 04-06hr    8 embryo 06-08hr    49 embryo 08-10hr    104 embryo 10-12hr    92 embryo 12-14hr    37 embryo 14-16hr    16 embryo 16-18hr    10 embryo 18-20hr    7 embryo 20-22hr    4 embryo 22-24hr    3 larva L1    5 larva L2    3 larva L3 12hr old    2 larva L3 puffstage 1-2    4 larva L3 puffstage 3-6    8 larva L3 puffstage 7-9    11 white prepupae new    12 white prepupae 12hr    17 white prepupae 24hr    19 pupae 2d postWPP    12 pupae 3d postWPP    7 pupae 4d postWPP    3 adult male 01day    2 adult male 05day    1 adult male 30day    1 adult female 01day    5 adult female 05day    2 adult female 30day    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    2 embryo 04-06hr    8 embryo 06-08hr  (49) embryo 08-10hr  (104) embryo 10-12hr  (92) embryo 12-14hr  (37) embryo 14-16hr    16 embryo 16-18hr    10 embryo 18-20hr    7 embryo 20-22hr    4 embryo 22-24hr    3 larva L1    5 larva L2    3 larva L3 12hr old    2 larva L3 puffstage 1-2    4 larva L3 puffstage 3-6    8 larva L3 puffstage 7-9    11 white prepupae new    12 white prepupae 12hr    17 white prepupae 24hr    19 pupae 2d postWPP    12 pupae 3d postWPP    7 pupae 4d postWPP    3 adult male 01day    2 adult male 05day    1 adult male 30day    1 adult female 01day    5 adult female 05day    2 adult female 30day    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    2 embryo 04-06hr    8 embryo 06-08hr    49 embryo 08-10hr  104 embryo 10-12hr    92 embryo 12-14hr    37 embryo 14-16hr    16 embryo 16-18hr    10 embryo 18-20hr    7 embryo 20-22hr    4 embryo 22-24hr    3 larva L1    5 larva L2    3 larva L3 12hr old    2 larva L3 puffstage 1-2    4 larva L3 puffstage 3-6    8 larva L3 puffstage 7-9    11 white prepupae new    12 white prepupae 12hr    17 white prepupae 24hr    19 pupae 2d postWPP    12 pupae 3d postWPP    7 pupae 4d postWPP    3 adult male 01day    2 adult male 05day    1 adult male 30day    1 adult female 01day    5 adult female 05day    2 adult female 30day    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    2 embryo 04-06hr    8 embryo 06-08hr    49 embryo 08-10hr    104 embryo 10-12hr    92 embryo 12-14hr    37 embryo 14-16hr    16 embryo 16-18hr    10 embryo 18-20hr    7 embryo 20-22hr    4 embryo 22-24hr    3 larva L1    5 larva L2    3 larva L3 12hr old    2 larva L3 puffstage 1-2    4 larva L3 puffstage 3-6    8 larva L3 puffstage 7-9    11 white prepupae new    12 white prepupae 12hr    17 white prepupae 24hr    19 pupae 2d postWPP    12 pupae 3d postWPP    7 pupae 4d postWPP    3 adult male 01day    2 adult male 05day    1 adult male 30day    1 adult female 01day    5 adult female 05day    2 adult female 30day    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    10 Schneider line 2 Sg4    1 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    1 embryonic S1    3 embryonic S3    2 leg disc CME L1    1 wing disc CME-W2    1 wing disc ML-DmD8    0 wing disc ML-DmD9    13 wing disc ML-DmD16-c3    6 wing disc ML-DmD21    14 wing disc ML-DmD32    1 haltere disc ML-DmD17-c3    1 eye-antennal disc ML-DmD11    8 antennal disc ML-DmD20-c5    1 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    2 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    0 ovary fGS/OSS    59 ovary OSC    39 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    10 Schneider line 2 Sg4    1 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    1 embryonic S1    3 embryonic S3    2 leg disc CME L1    1 wing disc CME-W2    1 wing disc ML-DmD8    0 wing disc ML-DmD9    13 wing disc ML-DmD16-c3    6 wing disc ML-DmD21    14 wing disc ML-DmD32    1 haltere disc ML-DmD17-c3    1 eye-antennal disc ML-DmD11    8 antennal disc ML-DmD20-c5    1 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    2 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    0 ovary fGS/OSS  (59) ovary OSC  (39) ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    10 Schneider line 2 Sg4    1 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    1 embryonic S1    3 embryonic S3    2 leg disc CME L1    1 wing disc CME-W2    1 wing disc ML-DmD8    0 wing disc ML-DmD9    13 wing disc ML-DmD16-c3    6 wing disc ML-DmD21    14 wing disc ML-DmD32    1 haltere disc ML-DmD17-c3    1 eye-antennal disc ML-DmD11    8 antennal disc ML-DmD20-c5    1 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    2 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    0 ovary fGS/OSS    59 ovary OSC    39 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    10 Schneider line 2 Sg4    1 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    1 embryonic S1    3 embryonic S3    2 leg disc CME L1    1 wing disc CME-W2    1 wing disc ML-DmD8    0 wing disc ML-DmD9    13 wing disc ML-DmD16-c3    6 wing disc ML-DmD21    14 wing disc ML-DmD32    1 haltere disc ML-DmD17-c3    1 eye-antennal disc ML-DmD11    8 antennal disc ML-DmD20-c5    1 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    2 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    0 ovary fGS/OSS    59 ovary OSC    39 ovary OSS    7 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    10 Schneider line 2 Sg4    1 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    1 embryonic S1    3 embryonic S3    2 leg disc CME L1    1 wing disc CME-W2    1 wing disc ML-DmD8    0 wing disc ML-DmD9    13 wing disc ML-DmD16-c3    6 wing disc ML-DmD21    14 wing disc ML-DmD32    1 haltere disc ML-DmD17-c3    1 eye-antennal disc ML-DmD11    8 antennal disc ML-DmD20-c5    1 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    2 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    0 ovary fGS/OSS    59 ovary OSC    39 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    10 Schneider line 2 Sg4    1 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    1 embryonic S1    3 embryonic S3    2 leg disc CME L1    1 wing disc CME-W2    1 wing disc ML-DmD8    0 wing disc ML-DmD9    13 wing disc ML-DmD16-c3    6 wing disc ML-DmD21    14 wing disc ML-DmD32    1 haltere disc ML-DmD17-c3    1 eye-antennal disc ML-DmD11    8 antennal disc ML-DmD20-c5    1 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    2 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    0 ovary fGS/OSS  (59) ovary OSC  (39) ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    10 Schneider line 2 Sg4    1 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    1 embryonic S1    3 embryonic S3    2 leg disc CME L1    1 wing disc CME-W2    1 wing disc ML-DmD8    0 wing disc ML-DmD9    13 wing disc ML-DmD16-c3    6 wing disc ML-DmD21    14 wing disc ML-DmD32    1 haltere disc ML-DmD17-c3    1 eye-antennal disc ML-DmD11    8 antennal disc ML-DmD20-c5    1 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    2 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    0 ovary fGS/OSS    59 ovary OSC    39 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    10 Schneider line 2 Sg4    1 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    1 embryonic S1    3 embryonic S3    2 leg disc CME L1    1 wing disc CME-W2    1 wing disc ML-DmD8    0 wing disc ML-DmD9    13 wing disc ML-DmD16-c3    6 wing disc ML-DmD21    14 wing disc ML-DmD32    1 haltere disc ML-DmD17-c3    1 eye-antennal disc ML-DmD11    8 antennal disc ML-DmD20-c5    1 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    2 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    0 ovary fGS/OSS    59 ovary OSC    39 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    4 cold shock, 4-day adult    2 heat shock, 4-day adult    2 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    1 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    1 Zinc 5 mM 12 hrs, larvae L3    6 Zinc 4.5 mM 48 hrs, 4-day adult    3 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    2 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    2 Paraquat 10 mM 48 hrs, 4-day adult    3 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    4 cold shock, 4-day adult    2 heat shock, 4-day adult    2 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    1 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    1 Zinc 5 mM 12 hrs, larvae L3    6 Zinc 4.5 mM 48 hrs, 4-day adult    3 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    2 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    2 Paraquat 10 mM 48 hrs, 4-day adult    3 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    4 cold shock, 4-day adult    2 heat shock, 4-day adult    2 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    1 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    1 Zinc 5 mM 12 hrs, larvae L3    6 Zinc 4.5 mM 48 hrs, 4-day adult    3 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    2 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    2 Paraquat 10 mM 48 hrs, 4-day adult    3 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    4 cold shock, 4-day adult    2 heat shock, 4-day adult    2 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    1 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    1 Zinc 5 mM 12 hrs, larvae L3    6 Zinc 4.5 mM 48 hrs, 4-day adult    3 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    2 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    2 Paraquat 10 mM 48 hrs, 4-day adult    3 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    4 cold shock, 4-day adult    2 heat shock, 4-day adult    2 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    1 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    1 Zinc 5 mM 12 hrs, larvae L3    6 Zinc 4.5 mM 48 hrs, 4-day adult    3 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    2 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    2 Paraquat 10 mM 48 hrs, 4-day adult    3 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    4 cold shock, 4-day adult    2 heat shock, 4-day adult    2 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    1 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    1 Zinc 5 mM 12 hrs, larvae L3    6 Zinc 4.5 mM 48 hrs, 4-day adult    3 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    2 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    2 Paraquat 10 mM 48 hrs, 4-day adult    3 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    4 cold shock, 4-day adult    2 heat shock, 4-day adult    2 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    1 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    1 Zinc 5 mM 12 hrs, larvae L3    6 Zinc 4.5 mM 48 hrs, 4-day adult    3 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    2 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    2 Paraquat 10 mM 48 hrs, 4-day adult    3 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    4 cold shock, 4-day adult    2 heat shock, 4-day adult    2 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    1 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    1 Zinc 5 mM 12 hrs, larvae L3    6 Zinc 4.5 mM 48 hrs, 4-day adult    3 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    2 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    2 Paraquat 10 mM 48 hrs, 4-day adult    3 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (Graveley et al., 2011) mE1_20_mRNA_expression_cluster_05
External Data & Images
Linkouts	BDGP expression data • RE19116 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0002609 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG8346-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | pupal stage, with Scer\GAL432B E(spl)m3-HLHScer\UAS.cLa Other Phenotypes Allele visible, with Scer\GAL432B E(spl)m3-HLHScer\UAS.cLa visible, with Scer\GAL4455.2 E(spl)m3-HLHScer\UAS.cLa visible, with Scer\GAL4ap-md544 E(spl)m3-HLHScer\UAS.cLa visible, with Scer\GAL4h-1J3 E(spl)m3-HLHScer\UAS.cLa Phenotype manifest in Allele scutellar bristle, with Scer\GAL4455.2 E(spl)m3-HLHScer\UAS.cLa scutum & macrochaeta, with Scer\GAL4ap-md544 E(spl)m3-HLHScer\UAS.cLa scutum & macrochaeta, with Scer\GAL4h-1J3 E(spl)m3-HLHScer\UAS.cLa scutum & microchaeta, with Scer\GAL432B E(spl)m3-HLHScer\UAS.cLa scutum & microchaeta, with Scer\GAL4ap-md544 E(spl)m3-HLHScer\UAS.cLa
Classical Alleles ( 2 )
For All Classical Alleles Show
Allele of E(spl)m3-HLH Class Mutagen Stocks Known lesion E(spl)m3-HLHd01076 P-element activity 1 -- E(spl)m3-HLHd05262 0 --
Alleles Carried on Transgenic Constructs ( 7 )
For All Alleles Carried on Transgenic Constructs Show
Allele of E(spl)m3-HLH Class Mutagen Stocks Known lesion E(spl)m3-HLHJF01999 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes E(spl)m3-HLH+t7.2 in vitro construct - genomic fragment 0 Yes E(spl)m3-HLHAct5C.PG in vitro construct - regulatory fusion 0 Yes E(spl)m3-HLHdsRNA.cDa in vitro construct - RNAi 0 Yes E(spl)m3-HLHdsRNA.cDb in vitro construct - RNAi 0 Yes E(spl)m3-HLHNIG.8346R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes E(spl)m3-HLHScer\UAS.cLa in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3R)boss16 (Heitzler et al., 1996) Df(3R)E(spl)-rv27 (Tietze et al., 1993, Heitzler et al., 1996, de Celis et al., 1996) Df(3R)E(spl)δ-6 (Chanet et al., 2009, Bardin et al., 2010) Df(3R)Espl4 (Heitzler et al., 1996) Df(3R)Exel6204 (Ryder, 2004.4.26) Df(3R)grob32.2 (Ligoxygakis et al., 1998, Heitzler et al., 1996, de Celis et al., 1996, de Celis et al., 1997) Df(3R)P11 (Nagel et al., 2000) Df(3R)Pr4 (Nagel et al., 2000) Df(3R)X10 (Nagel et al., 2000)
Duplicated in	Dp(3;2)E(spl)δ-8 (Chanet et al., 2009) Dp(3;2)E(spl)δ-8Δα46 (Chanet et al., 2009)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EP}EP3635 NA P{XP}E(spl)m3-HLHd01076 NA P{XP}E(spl)m3-HLHd05262 NA
Gene Ontology: Function, Process & Cellular Component ( 9 unique terms )
Terms Based on Experimental Evidence ( 1 term )
Molecular Function
CV term Evidence References
sequence-specific DNA binding inferred from direct assay (Jennings et al., 1999)
Biological Process ( 0 terms)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 8 terms )
Molecular Function
CV term Evidence References
DNA binding non-traceable author statement (Swiss-Prot Project Members, 2000.10.1) protein dimerization activity inferred from electronic annotation with InterPro:IPR011598 (FlyBase Curators et al., 2004-) sequence-specific DNA binding transcription factor activity non-traceable author statement (FlyBase, 1992-) traceable author statement (Kumar and Moses, 2000)
Biological Process
CV term Evidence References
compound eye development traceable author statement (Kumar and Moses, 2000) negative regulation of transcription from RNA polymerase II promoter non-traceable author statement (Swiss-Prot Project Members, 2000.10.1) Notch signaling pathway non-traceable author statement (Swiss-Prot Project Members, 2000.10.1) regulation of transcription, DNA-dependent inferred from electronic annotation with InterPro:IPR003650 (FlyBase Curators et al., 2004-)
Cellular Component
CV term Evidence References
nucleus non-traceable author statement (FlyBase, 1992-, Swiss-Prot Project Members, 2000.10.1)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
E(spl)m3-HLH - B52 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) E(spl)m3-HLH - CG14207 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) E(spl)m3-HLH - CG15784 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) E(spl)m3-HLH - CG2199 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) E(spl)m3-HLH - CG7878 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) E(spl)m3-HLH - Chc anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) E(spl)m3-HLH - Clc anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) E(spl)m3-HLH - Jupiter anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) E(spl)m3-HLH - NHP2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) E(spl)m3-HLH - RpLP0 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) E(spl)m3-HLH - Snr1 experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) E(spl)m3-HLH - bic anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) E(spl)m3-HLH - dgrn pull down, autoradiography (Barry et al., 2011) E(spl)m3-HLH - mor anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) E(spl)m3-HLH - nop5 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) E(spl)m3-HLH - p38b anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions E(spl)m3-HLH allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 68056 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0002609 DroID - A comprehensive database of gene and protein interactions. • FBgn0002609 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 43156
Orthologs
OrthoDB Orthologs (15) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6NGH74 Dipteran inclusive ortholog search No orthologs identified Insect inclusive ortholog search No orthologs identified Arthropod inclusive ortholog search EOG6WH73C Metazoa inclusive ortholog search No orthologs identified
Orthologs in Drosophila Species (EOG6NGH74)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\E(spl)m3-HLH     Drosophila simulans   Dsim\HLHm3 Y   Drosophila sechellia   Dsec\GM10297 Y   Drosophila erecta   Dere\GG11454 Y   Drosophila yakuba   Dyak\GE23647 Y   Drosophila ananassae   Dana\GF16799 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA21008 Y   Drosophila persimilis   Dper\GL21819 Y   Drosophila willistoni   Dwil\GK14458 Y   Drosophila virilis   Dvir\GJ23906 Y   Drosophila mojavensis   Dmoj\GI24690 Y   Drosophila grimshawi   Dgri\GH19456 Y
Orthologs in non-Drosophila Dipterans (None identified)
No non-Drosophilid orthologies identified
Orthologs in non-Dipteran Insects (None identified)
No non-Dipteran orthologies identified
Orthologs in non-Insect Arthropods (EOG6WH73C)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\Mterfd1 Y Daphnia pulex Water flea  Dpux\JGI_V11_64307 Y Daphnia pulex Water flea  Dpux\cytb Y
Orthologs in non-Arthropod Metazoa (None identified)
No non-Arthropod Metazoa orthologies identified
Human Orthologs (0)
Gene OMIM HGNC
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\HLHm3 Drosophila sechellia Dsec\GM10297 Drosophila erecta Dere\GG11454 Drosophila yakuba Dyak\GE23647 Drosophila ananassae Dana\GF16799 Drosophila pseudoobscura pseudoobscura Dpse\GA21008 Drosophila persimilis Dper\GL21819 Drosophila willistoni Dwil\GK14458 Drosophila virilis Dvir\GJ23906 Drosophila mojavensis Dmoj\GI24690 Drosophila grimshawi Dgri\GH19456
Stocks & Reagents
Stocks Listed in FlyBase ( 2 )
Bloomington	25977 y1 v1; P{TRiP.JF01999}attP2
Harvard	- P{XP}E(spl)m3-HLHd01076
Genomic Clones ( 2 )
	BACR13F13 BACR22N13 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 51 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	RE19116
Other clones	DPiM_RE19116
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	RE08505
Other clones	84092 84320 107703 120042 273882 CK01.5B.E4 CK01.6A.F10 CK01.97D.G6 CK01.104B.C11 CK01.108A.G6 CK01.108A.H10 CK01.113B.G3 CK01.144C.A4 EK075759 EK082059 EK087510 EK092159 EK092261 EK119134 EK218714 EK240029 EK242229 RP003038634 RP003079855 RP003092808 RP003094005 RP003135718 RP003156581 RP003231946 RP003235635 RP003257321 RP003271215 RP003277720 RP003392162 RP003398007 RP003402371 RP003411983 RP003455548 RP003484095 RP003495176 RP003501432 RP003510608 RP003513474 RP003531721 RP003549066 RP003549618 RP003559592 RP003569504
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0002609 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 43156
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: E(spl)m3-HLH HLHm3 (Artavanis-Tsakonas et al., 2012.8.8)
Source for database merge of
Additional comments
Other Comments
	The distinct expression patterns of genes of the E(spl) complex in imaginal tissues depend to a significant degree on the capacity of their transcriptional cis-regulatory apparatus to respond selectively to direct proneural and Su(H)-mediated activation, often in a subset of the territories and cells in which proneural and Su(H) regulation is occurring. (Nellesen et al., 1999) The bHLH domains of the gene products encoded by the E(spl)-C and AS-C differ in their ability to form homo- and/or heterodimers. The interactions established through the bHLH link the products of the two complexes in a single interaction network which may function to ensure that a given cell retains the capacity to choose between epidermoblast and neuroblast fates until the cell becomes definitively determined. (Gigliani et al., 1996) Clones mutant for E(spl)-C bHLH-encoding genes or for gro display bristle hyperplasia. (Heitzler et al., 1996) E(spl)-C mutants are epistatic over a gain of function mutant of N and ac-sc mutants are epistatic over E(spl)-C mutants. (Heitzler et al., 1996) The E(spl)-C genes participate in the N signalling pathway. (Heitzler et al., 1996) Almost all E(spl)-complex bHLH proteins can homo-hetero-dimerise, but not with the same efficiency. All E(spl)-complex bHLH proteins interact with gro protein via their C-terminal domain. E(spl)-complex bHLH proteins interact with proneural proteins, with members of the E(spl) family exhibiting distinct preferences for different proneural proteins. (Alifragis et al., 1995) The bristle loss phenotype of H mutants can be suppressed by deleting components of the E(spl)-complex. The degree of suppression depends on both the number and identity of E(spl)-complex transcription units removed. (Bang et al., 1995) Arrangement and sequence of E(spl)-complex genes in D.melanogaster and D.hydei revealed that the E(spl)-gene, and the structure of complex are highly conserved, suggesting that each individual gene, as well as the organization of the complex, is of functional importance. (Maier et al., 1993) E(spl) region gene encoding HLH protein identified by low stringency hybridization to previously defined HLHm5 and HLHm7 probes. On basis of cross-hybridization and sequence data the E(spl) HLH genes can be placed into 3 groups. The first includes E(spl) and HLHm5, the second includes HLHm7, HLHm3, HLHmβ and HLHmγ and the last includes HLHmδ. (Delidakis and Artavanis-Tsakonas, 1992) HLHm3, HLHmβ, HLHmγ and HLHmδ encode helix-loop-helix proteins, bringing the total of such proteins in the E(spl) complex to seven. (Knust et al., 1992) Genes of the E(spl) complex act as a functional unit composed of redundant genes which can partially substitute for each other. Eight E(spl)-region genes are required for the development of neurectodermal cells: HLHmδ, HLHmβ, HLHmγ, HLHm3, HLHm5, HLHm7, E(spl) and gro. The E(spl)-region gene m4 may also play a role in this process. (Schrons et al., 1992)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_079785 RefSeq (Proteins) • NP_524509 DDBJ / EMBL / GenBank • AAA28908 AAF56550 AAM11211 AAV59048 AAV59060 AAV59072 AAV59084 AAV59096 AAV59108 AAV59120 AAV59132 AAV59144 AAV59156 AAV59168 AAV59180 AAV59192 AAV59204 AAV59216 AAV59228 AAX60605 AAX60606 AAX60607 AAX60608 AAX60609 AAX60610 AAX60611 AAX60612 AAX60613 AAX60614 AAX60615 AAX60616 AAX60617 AAX60618 AAX60619 AAX60620 AAX60621 AAX60622 AAX60623 AAX60624 AAX60625 AAX60626 AAX60627 AAX60628 AAX60629 AAX60630 AAX60631 AAX60632 AAX60633 AAX60634 AAX60635 AAX60636 AAX60637 AAX60638 AAX60639 AAX60640 AAX60641 AAX60642 AAX60643 AAX60644 AAX60645 AAX60646 AAX60647 AAX60648 AAX60649 AAX60650 AAX60651 AAX60652 AAX60653 AAX60654 AAX60655 AAX60656 AAX60657 AAX60658 AAX60659 AAX60660 AAX60661 AAX60662 AAX60663 AAX60664 AAX60665 AAX60666 AAX60667 AAX60668 AAX60669 AAX60670 AAX60671 AAX60672 AAX60673 AAX60674 AAX60675 AAX60676 AAX60677 AAX60678 AAX60679 AAX60680 AAX60681 AAX60682 AAX60683 AAX60684 AAX60685 AAX60686 AAX60687 AAX60688 AAX60689 AAX60690 AAX60691 AAX60692 AAX60693 AAX60694 AAX60695 AF115454 AY094858 AY779906 AY779907 AY779908 AY779909 AY779910 AY779911 AY779912 AY779913 AY779914 AY779915 AY779916 AY779917 AY779918 AY779919 AY779920 AY779921 AY906259 AY906260 AY906261 AY906262 AY906263 AY906264 AY906265 AY906266 AY906267 AY906268 AY906269 AY906270 AY906271 AY906272 AY906273 AY906274 AY906275 AY906276 AY906277 AY906278 AY906279 AY906280 AY906281 AY906282 AY906283 AY906284 AY906285 AY906286 AY906287 AY906288 AY906289 AY906290 AY906291 AY906292 AY906293 AY906294 AY906295 AY906296 AY906297 AY906298 AY906299 AY906300 AY906301 AY906302 AY906303 AY906304 AY906305 AY906306 AY906307 AY906308 AY906309 AY906310 AY906311 AY906312 AY906313 AY906314 AY906315 AY906316 AY906317 AY906318 AY906319 AY906320 AY906321 AY906322 AY906323 AY906324 AY906325 AY906326 AY906327 AY906328 AY906329 AY906330 AY906331 AY906332 AY906333 AY906334 AY906335 AY906336 AY906337 AY906338 AY906339 AY906340 AY906341 AY906342 AY906343 AY906344 AY906345 AY906346 AY906347 AY906348 AY906349 BI213666 CAA47431 CZ472012 M96165 X67046 Entrez Gene - A searchable database of RefSeq genes. • 43156 UniProtKB/Swiss-Prot • Q01068 UniProtKB/TrEMBL • Q56LA3 Q56LC9 Q56LE8 Q56LI3
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Orange subgroup (IPR018352) Orange (IPR003650) Myc-type, basic helix-loop-helix (bHLH) domain (IPR011598) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • RE19116
Linkouts
	BioGRID - A database of protein and genetic interactions • 68056 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0002609 DroID - A comprehensive database of gene and protein interactions. • FBgn0002609 DRSC - Results from RNAi screens. • FBgn0002609 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0002609 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG8346-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0002609 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 43156 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 43156 modMine - Data generated by the modENCODE project. • FBgn0002609
Synonyms & Secondary IDs ( 17 )
Reported As
Symbol Synonym	bHLHc14 (Stevens et al., 2008) CG8346 (Bhutkar et al., 2008, Perkins et al., 1.15.2009, Stevens et al., 2008, Stevens et al., 2008, Krejcí et al., 2009) E(spl)C (Ahimou et al., 2004, Bose et al., 2008) E(spl)C m3 (Ahimou et al., 2004, Mok et al., 2005, Bardot et al., 2005) E(Spl)-HLH-m3 (Knust, 1992.6.22) E(spl)-m3 (Le Borgne et al., 2005) E(spl)m3 (Lai et al., 2005, Rebeiz et al., 2002, Lai, 2002, Rosenberg and Parkhurst, 2002, Brody et al., 2002, Ledent and Vervoort, 2001, Nellesen, 1998.12.18, Ligoxygakis et al., 1999, Lai et al., 1998, Fisher and Caudy, 1998, Leviten et al., 1997, Gallant et al., 1996, Barry et al., 2011, Duan et al., 2011, Goodfellow et al., 2007, Krejcí et al., 2009, Bernard et al., 2010, Endo et al., 2011, Leonardi et al., 2011, Engel et al., 2012) E(spl)M3 (Jennings et al., 1999) E(spl)m3-HLH   HLHM3 (Morgenstern and Atchley, 1999) HLH-m3 (Tietze et al., 1993, Campos-Ortega, 1993) HLHm3 (Fay et al., 2011, Japanese National Institute of Genetics, 2012.5.21, Dorner et al., 2006, Blanco et al., 2010, Kadener et al., 2007, Maeder et al., 2007, Johnson et al., 2011, Toku et al., 2011, Sandmann et al., 2006, Lu et al., 2008, Schaaf et al., 2009, Zeitlinger et al., 2007, Duncan and Dearden, 2010, Cunha et al., 2010) m3 (Bhutkar et al., 2008, Macdonald et al., 2005, Lai et al., 2005, Giagtzoglou et al., 2005, Kan and Kessler, 2005, Secombe and Parkhurst, 2004, Trott et al., 2001, Kumar and Moses, 2000, Lai et al., 2000, Nellesen et al., 1999, Wech et al., 1999, Wurmbach et al., 1999, Preiss et al., 1997, Ligoxygakis et al., 1998, Lai and Posakony, 1998, de Celis et al., 1996, Gigliani et al., 1996, Heitzler et al., 1996, Hartenstein et al., 1996, Bang et al., 1995, Alifragis et al., 1995, Alifragis et al., 1995, Tepass and Hartenstein, 1995, Tietze et al., 1993, Schrons et al., 1991, Delidakis and Artavanis-Tsakonas, 1992, Campos-Ortega and Knust, 1990, Campos-Ortega and Knust, 1990, Eastman et al., 2007, Ahimou et al., 2004, Nagel et al., 2004, Schlatter and Maier, 2005, Krejci and Bray, 2007, Macdonald and Long, 2005, Maeder et al., 2009, Chanet et al., 2009, Bardin et al., 2010, Bernard et al., 2010, Duncan and Dearden, 2010, Cave et al., 2011, Rand et al., 2008)
Name Synonym	E(spl) region transcript m3 (Krejcí et al., 2009, Rehmsmeier et al., 2004) Enhancer of split m3 (Ahimou et al., 2004, Wesley and Mok, 2003) Enhancer of split m3, helix-loop-helix   split locus enhancer protein m3 (Delidakis, 1992.9.29)
Secondary FlyBase IDs
	FBgn0010046
References ( 130 )
Generate a list of	All references relating to this gene ( 130 )
List References by type	Research paper  ( 89 ) Supplementary material  ( 5 ) Review  ( 9 ) Personal communication to FlyBase  ( 8 ) Abstract  ( 8 ) FlyBase analysis  ( 2 ) DNA/RNA sequence record  ( 3 ) Letter  ( 1 ) Stock list  ( 1 ) Protein sequence record  ( 1 ) Website  ( 1 ) Curated genome annotation  ( 2 )
Recent research papers ( 12 )
	Housden et al., 2013, PLoS Genet. 9(1): e1003162 Transcriptional Dynamics Elicited by a Short Pulse of Notch Activation Involves Feed-Forward Regulation by E(spl)/Hes Genes. [FBrf0220474] Engel et al., 2012, Toxicol. In Vitro 26(3): 485--492 The effects of methylmercury on Notch signaling during embryonic neural development in Drosophila melanogaster. [FBrf0217650] Baker et al., 2011, BMC Evol. Biol. 11: 354 The Enhancer of split complex arose prior to the diversification of schizophoran flies and is strongly conserved between Drosophila and stalk-eyed flies (Diopsidae). [FBrf0217296] Barry et al., 2011, Development 138(9): 1759--1769 The Drosophila STUbL protein Degringolade limits HES functions during embryogenesis. [FBrf0213458] Cave et al., 2011, Mol. Cell. Biol. 31(1): 22--29 Differential Regulation of Transcription through Distinct Suppressor of Hairless DNA Binding Site Architectures during Notch Signaling in Proneural Clusters. [FBrf0212524] Duan et al., 2011, EMBO J. 30(15): 3120--3133 Insensitive is a corepressor for Suppressor of Hairless and regulates Notch signalling during neural development. [FBrf0214638] Endo et al., 2011, Nat. Neurosci. 15(2): 224--233 Chromatin modification of Notch targets in olfactory receptor neuron diversification. [FBrf0217319] Fay et al., 2011, Curr. Biol. 21(19): 1624--1634 Cohesin selectively binds and regulates genes with paused RNA polymerase. [FBrf0216412] Johnson et al., 2011, Development 138(21): 4627--4638 JAK/Stat signaling regulates heart precursor diversification in Drosophila. [FBrf0216404] Leonardi et al., 2011, Development 138(16): 3569--3578 Multiple O-glucosylation sites on Notch function as a buffer against temperature-dependent loss of signaling. [FBrf0214548] Mulligan et al., 2011, Mol. Cell 42(5): 689--699 A SIRT1-LSD1 Corepressor Complex Regulates Notch Target Gene Expression and Development. [FBrf0213865] Toku et al., 2011, Comput. Biol. Chem. 35(5): 282--292 Reconstruction and crosstalk of protein-protein interaction networks of Wnt and Hedgehog signaling in Drosophila melanogaster. [FBrf0216393] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...