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Gene Dmel\RpL32

General Information
Symbol	Dmel\RpL32	Species	D. melanogaster
Name	Ribosomal protein L32	Annotation symbol	CG7939
Feature type	protein_coding_gene	FlyBase ID	FBgn0002626
Gene Model Status	Current	Stock availability	7 publicly available
Also Known As	rp49, M(3)99D, Rp-49, Rp49/RpL32
Genomic Location
Chromosome (arm)	3R	Recombination map
Cytogenetic map	99D3-99D3	Sequence location	3R:25,870,951..25,871,880 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Ribosomal protein L32 is referred to in FlyBase by the symbol Dmel\RpL32 (CG7939, FBgn0002626). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: structural constituent of ribosome. There is experimental evidence that it is involved in the biological process: mitotic spindle elongation; mitotic spindle organization. 5 alleles are reported. No phenotypic data is available. It has 5 annotated transcripts and 5 annotated polypeptides. Protein features are: Ribosomal protein L32e; Ribosomal protein L32e, conserved site. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of extremely high expression to a trough of very high expression. Peak expression observed at stages throughout embryogenesis, at stages throughout the larval period, in adult female stages. Summary of FlyAtlas Anatomical Expression Data: High levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male reproductive system, larval/adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1625337_s_at partially aligns to an exonic region common to each of the 5 FlyBase-annotated transcript isoforms of RpL32: 13 out of 14 probes fall into this exonic region. Gene sequence location is 3R:25870951..25871880.
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	M(3)99D Identified by the phenotype of heterozygotes for a synthetic deficiency; no mutant alleles identified. Strong Minute; small bristles, slightly abnormal wings; eclosion delayed two to three days (Kongsuwan, Dellavalle, and Merriam, 1986, Genetics 112: 539-50). M(3)99D/+ females produce smaller than normal eggs; 10-15% of embryos fail to hatch often displaying fusion and partial deletion of segments postrior to A3 (Kongsuwan, Yu, Vincent, Frisardi, Rosbash, Lengyell, and Merriam, 1985, Nature 317: 555-58).
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2012_01	Sequence features BKN20525 HFA16835
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 99D3-99D3   Limits computationally determined from genome sequence between P{PZ}ncd05884 and P{lacW}l(3)s2500s2500
Experimentally Determined Cytological Location
Cytogenetic map Notes References 99D1-99D9   (determined by in situ hybridisation)   (Ranz et al., 1997) 99D-99D   (determined by in situ hybridisation)   (Segarra et al., 1996) 99D-99D   (determined by in situ hybridisation)   (Lozovskaya et al., 1993) 99D1-99D9   (determined by in situ hybridisation) 99D2--9 (determined by in situ hybridisation)   (Kongsuwan et al., 1985)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\RpL32 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) RpL32-RA FBtr0085594  NM_079843   507   134 RpL32-RB FBtr0085592  NM_170461   521   134 RpL32-RC FBtr0085593  NM_170460   747   147 RpL32-RD FBtr0114555  NM_001144655   736   134 RpL32-RE FBtr0114556  NM_001144656   536   134
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein RpL32-PA   FBpp0084960   16.0   134   11.95     NP_524582   AAF57001 RpL32-PB   FBpp0084958   16.0   134   11.95     NP_733340   AAN14211 RpL32-PC   FBpp0084959   17.4   147   11.64     NP_733339   AAN14210 RpL32-PD   FBpp0113047   16.0   134   11.95     NP_001138127   ACL83583 RpL32-PE   FBpp0113048   16.0   134   11.95     NP_001138128   ACL83584
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Ribosomal protein L32e, conserved site (IPR018263) Ribosomal protein L32e (IPR001515)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA804108   AA804125   AA817589   AE014297 AAF57001   AE014297 AAN14210   AE014297 AAN14211   AE014297 ACL83583   AE014297 ACL83584   AI107582   AI108169   AI113364   AI113448   AI134103   AI134527   AI135380   AI238005   AI257263   AI257645   AI258442   AI258998   AI294277   AI295424   AI295502   AI297513   AI297536   AI297851   AI389542   AI402426   AI403634   AI404073   AI405234   AI514174   AI517978   AI519847   AI546313   AI946722   AY070656 AAL48127   AY231887   AY232069   BE978146   BG636230   BG638692   BG640602   BI142187   BI163790   BI165627   BI166869   BI167936   BI168694   BI170253   BI170661   BI213759   BI215537   BI231331   BI232291   BI232745   BI232746   BI232933   BI234070   BI240589   BI242172   BI351628   BI354116   BI356316   BI362790   BI364923   BI365073   BI370246   BI371426   BI372677   BI486981   BI564510   BI564570   BI565953   BI566049   BI566366   BI566368   BI566615   BI567158   BI567650   BI568365   BI568957   BI568958   BI570450   BI571060   BI571088   BI571475   BI572251   BI572431   BI572628   BI572947   BI574460   BI574482   BI574857   BI576975   BI581341   BI581368   BI581413   BI583318   BI583933   BI583961   BI584221   BI584453   BI584492   BI585585   BI585795   BI587139   BI587217   BI587284   BI587339   BI587361   BI589296   BI589915   BI590335   BI590718   BI593585   BI604753   BI607987   BI607996   BI610032   BI611951   BI612744   BI613120   BI614667   BI615225   BI615365   BI615440   BI617803   BI618108   BI619272   BI619857   BI620036   BI621290   BI621909   BI622794   BI623771   BI624425   BI625297   BI625977   BI626306   BI626314   BI626444   BI626667   BI630188   BI630469   BI630802   BI631956   BI634420   BI637646   BP540726   BP541178   BP542074   BP544301   BP544302   BP545681   BP546573   BP547693   BP548653   BP548654   BP553897   BP553898   BP554615   BP554616   BP554617   BP554618   BT011442   BT030157   BX007253   BX007798   BX008500   BX012079   BX012521   BX016586   BX016591   BX017413   BX021654   BX022072   BX023560   BX024689   BX024698   BX025196   BX028334   BX028659   BX030137   BX031229   BX031402   BX031670   BX034018   BX035080   BX035454   BX036679   BX037616   BX037741   BX037881   BX038212   BX038238   BX038957   BX042256   BX043216   BX047905   BX053725   BX054318   BX060492   BX069104   BX072246   BX072362   BX072405   CA803304   CA803538   CA805529   CA805817   CK660774   CK661795   CO152816   CO153256   CO153514   CO153646   CO154078   CO155152   CO156189   CO156201   CO156611   CO179116   CO181188   CO181516   CO182088   CO182250   CO183086   CO183095   CO183278   CO184162   CO185098   CO188163   CO190914   CO191413   CO191595   CO193023   CO193359   CO194282   CO194523   CO194644   CO194806   CO195017   CO195466   CO196525   CO263086   CO266078   CO266157   CO266533   CO284018   CO286953   CO288762   CO302518   CO308530   CO315913   CO316818   CO319120   CO326822   CO335444   CO335593   CO335842   CO336352   CO336525   CO336740   CO337107   CO337943   CO338772   DQ440260   EC066915   EC067055   EC067056   EC067898   EC070012   EC073000   EC076251   EC076452   EC076480   EC077897   EC084708   EC084998   EC089959   EC195190   EC195898   EC196144   EC197845   EC204059   EC204594   EC207579   EC208040   EC208443   EC210097   EC210348   EC211206   EC215062   EC218378   EC226907   EC227890   EC227951   EC231207   EC231208   EC233994   EC234112   EC240365   EC240736   EC242081   EC243644   EC244916   EC245432   EC248156   EC248364   EC259072   EC261694   EC261736   EC261783   EC262142   EC262326   EC262407   EC262494   EC263016   EC263448   EC263737   EC264722   EC264833   EC264954   EC265093   EC266364   EC266380   EC266688   EC266806   EC267841   EC267872   EL880537   EV575815   EV576450   EV576802   EV577262   EV577365   EV577580   EV577998   EV579018   EV580046   EV580340   EV580577   EV581351   EV581474   EV581863   EV581956   EV582533   EV583325   EV585153   EV585835   EV586008   EV587832   EV587870   EV590924   EV592262   EV592437   EV593234   EV593398   EV595525   EV595569   EV595692   EV595758   EV596226   EV596740   EV597276   EV598980   EV599469   GH943930   GH945750   GH946112   GH946561   GH947857   GH947956   GH948084   GH948108   GH949511   GH949565   GH949677   GH950700   GH951030   GH951569   GH951610   GH951724   GH951937   GH952794   GH952819   GH953034   GH957201   GH957602   GH957862   GH959607   GH960023   GH960374   GH960740   GH960775   GH961285   GH961809   GH962486   GH963539   GH963642   GH964004   GH964355   GH965143   GH967073   GH969089   GH970956   GH971760   GH972901   GH973383   GH974545   GH976293   GH976391   GH977247   GH977524   GH978469   GH978497   GH979080   GH979093   GH979488   GH980642   GH983261   GH983756   GH984255   GH984559   U92431 AAB51389   X00848 CAA25404   Y13939 CAA74278
UniProtKB/Swiss-Prot	P04359
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences	EPD - Eukarytoic Promoter Database, an annotated collection of POL II promoters • 07035
Expression Data
Transcript Expression
Additional Descriptive Data
	RpL32 transcript levels are unaffected by changes in dietary sucrose, glucose, fructose, starch, or ethanol. (Lissemore et al., 1990)
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
mass spectroscopy Stage Tissue/Position (including subcellular localization) Reference day 7 of adulthood -- day 49 of adulthood   adult heart   (Cammarato et al., 2011)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0002626    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0002626 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of extremely high expression to a trough of very high expression. Peak expression observed at stages throughout embryogenesis, at stages throughout the larval period, in adult female stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0002626 expression level Developmental Stage   Expression Level embryo 00-02hr    105761 embryo 02-04hr    155990 embryo 04-06hr    153970 embryo 06-08hr    170364 embryo 08-10hr    182390 embryo 10-12hr    212385 embryo 12-14hr    131258 embryo 14-16hr    172638 embryo 16-18hr    144228 embryo 18-20hr    167290 embryo 20-22hr    179162 embryo 22-24hr    141779 larva L1    220934 larva L2    160663 larva L3 12hr old    129084 larva L3 puffstage 1-2    108176 larva L3 puffstage 3-6    101593 larva L3 puffstage 7-9    97207 white prepupae new    93637 white prepupae 12hr    81795 white prepupae 24hr    78097 pupae 2d postWPP    76344 pupae 3d postWPP    62874 pupae 4d postWPP    75949 adult male 01day    51511 adult male 05day    55638 adult male 30day    70823 adult female 01day    135017 adult female 05day    104138 adult female 30day    115171 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (105761) embryo 02-04hr  (155990) embryo 04-06hr  (153970) embryo 06-08hr  (170364) embryo 08-10hr  (182390) embryo 10-12hr  (212385) embryo 12-14hr  (131258) embryo 14-16hr  (172638) embryo 16-18hr  (144228) embryo 18-20hr  (167290) embryo 20-22hr  (179162) embryo 22-24hr  (141779) larva L1  (220934) larva L2  (160663) larva L3 12hr old  (129084) larva L3 puffstage 1-2  (108176) larva L3 puffstage 3-6  (101593) larva L3 puffstage 7-9  (97207) white prepupae new  (93637) white prepupae 12hr  (81795) white prepupae 24hr  (78097) pupae 2d postWPP  (76344) pupae 3d postWPP  (62874) pupae 4d postWPP  (75949) adult male 01day  (51511) adult male 05day  (55638) adult male 30day  (70823) adult female 01day  (135017) adult female 05day  (104138) adult female 30day  (115171) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (105761) embryo 02-04hr  (155990) embryo 04-06hr  (153970) embryo 06-08hr  (170364) embryo 08-10hr  (182390) embryo 10-12hr  (212385) embryo 12-14hr  (131258) embryo 14-16hr  (172638) embryo 16-18hr  (144228) embryo 18-20hr  (167290) embryo 20-22hr  (179162) embryo 22-24hr  (141779) larva L1  (220934) larva L2  (160663) larva L3 12hr old  (129084) larva L3 puffstage 1-2  (108176) larva L3 puffstage 3-6  (101593) larva L3 puffstage 7-9  (97207) white prepupae new  (93637) white prepupae 12hr  (81795) white prepupae 24hr  (78097) pupae 2d postWPP  (76344) pupae 3d postWPP  (62874) pupae 4d postWPP  (75949) adult male 01day  (51511) adult male 05day  (55638) adult male 30day  (70823) adult female 01day  (135017) adult female 05day  (104138) adult female 30day  (115171) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr  (105761) embryo 02-04hr  (155990) embryo 04-06hr  (153970) embryo 06-08hr  (170364) embryo 08-10hr  (182390) embryo 10-12hr  (212385) embryo 12-14hr  (131258) embryo 14-16hr  (172638) embryo 16-18hr  (144228) embryo 18-20hr  (167290) embryo 20-22hr  (179162) embryo 22-24hr  (141779) larva L1  (220934) larva L2  (160663) larva L3 12hr old  (129084) larva L3 puffstage 1-2  (108176) larva L3 puffstage 3-6  (101593) larva L3 puffstage 7-9  (97207) white prepupae new  (93637) white prepupae 12hr  (81795) white prepupae 24hr  (78097) pupae 2d postWPP  (76344) pupae 3d postWPP  (62874) pupae 4d postWPP  (75949) adult male 01day  (51511) adult male 05day  (55638) adult male 30day  (70823) adult female 01day  (135017) adult female 05day  (104138) adult female 30day  (115171) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    105761 embryo 02-04hr    155990 embryo 04-06hr    153970 embryo 06-08hr    170364 embryo 08-10hr    182390 embryo 10-12hr    212385 embryo 12-14hr    131258 embryo 14-16hr    172638 embryo 16-18hr    144228 embryo 18-20hr    167290 embryo 20-22hr    179162 embryo 22-24hr    141779 larva L1    220934 larva L2    160663 larva L3 12hr old    129084 larva L3 puffstage 1-2    108176 larva L3 puffstage 3-6    101593 larva L3 puffstage 7-9    97207 white prepupae new    93637 white prepupae 12hr    81795 white prepupae 24hr    78097 pupae 2d postWPP    76344 pupae 3d postWPP    62874 pupae 4d postWPP    75949 adult male 01day    51511 adult male 05day    55638 adult male 30day    70823 adult female 01day    135017 adult female 05day    104138 adult female 30day    115171 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0002626 expression level Developmental Stage   Expression Level embryo 00-02hr    105761 embryo 02-04hr    155990 embryo 04-06hr    153970 embryo 06-08hr    170364 embryo 08-10hr    182390 embryo 10-12hr    212385 embryo 12-14hr    131258 embryo 14-16hr    172638 embryo 16-18hr    144228 embryo 18-20hr    167290 embryo 20-22hr    179162 embryo 22-24hr    141779 larva L1    220934 larva L2    160663 larva L3 12hr old    129084 larva L3 puffstage 1-2    108176 larva L3 puffstage 3-6    101593 larva L3 puffstage 7-9    97207 white prepupae new    93637 white prepupae 12hr    81795 white prepupae 24hr    78097 pupae 2d postWPP    76344 pupae 3d postWPP    62874 pupae 4d postWPP    75949 adult male 01day    51511 adult male 05day    55638 adult male 30day    70823 adult female 01day    135017 adult female 05day    104138 adult female 30day    115171 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (105761) embryo 02-04hr  (155990) embryo 04-06hr  (153970) embryo 06-08hr  (170364) embryo 08-10hr  (182390) embryo 10-12hr  (212385) embryo 12-14hr  (131258) embryo 14-16hr  (172638) embryo 16-18hr  (144228) embryo 18-20hr  (167290) embryo 20-22hr  (179162) embryo 22-24hr  (141779) larva L1  (220934) larva L2  (160663) larva L3 12hr old  (129084) larva L3 puffstage 1-2  (108176) larva L3 puffstage 3-6  (101593) larva L3 puffstage 7-9  (97207) white prepupae new  (93637) white prepupae 12hr  (81795) white prepupae 24hr  (78097) pupae 2d postWPP  (76344) pupae 3d postWPP  (62874) pupae 4d postWPP  (75949) adult male 01day  (51511) adult male 05day  (55638) adult male 30day  (70823) adult female 01day  (135017) adult female 05day  (104138) adult female 30day  (115171) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (105761) embryo 02-04hr  (155990) embryo 04-06hr  (153970) embryo 06-08hr  (170364) embryo 08-10hr  (182390) embryo 10-12hr  (212385) embryo 12-14hr  (131258) embryo 14-16hr  (172638) embryo 16-18hr  (144228) embryo 18-20hr  (167290) embryo 20-22hr  (179162) embryo 22-24hr  (141779) larva L1  (220934) larva L2  (160663) larva L3 12hr old  (129084) larva L3 puffstage 1-2  (108176) larva L3 puffstage 3-6  (101593) larva L3 puffstage 7-9  (97207) white prepupae new  (93637) white prepupae 12hr  (81795) white prepupae 24hr  (78097) pupae 2d postWPP  (76344) pupae 3d postWPP  (62874) pupae 4d postWPP  (75949) adult male 01day  (51511) adult male 05day  (55638) adult male 30day  (70823) adult female 01day  (135017) adult female 05day  (104138) adult female 30day  (115171) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr  (105761) embryo 02-04hr  (155990) embryo 04-06hr  (153970) embryo 06-08hr  (170364) embryo 08-10hr  (182390) embryo 10-12hr  (212385) embryo 12-14hr  (131258) embryo 14-16hr  (172638) embryo 16-18hr  (144228) embryo 18-20hr  (167290) embryo 20-22hr  (179162) embryo 22-24hr  (141779) larva L1  (220934) larva L2  (160663) larva L3 12hr old  (129084) larva L3 puffstage 1-2  (108176) larva L3 puffstage 3-6  (101593) larva L3 puffstage 7-9  (97207) white prepupae new  (93637) white prepupae 12hr  (81795) white prepupae 24hr  (78097) pupae 2d postWPP  (76344) pupae 3d postWPP  (62874) pupae 4d postWPP  (75949) adult male 01day  (51511) adult male 05day  (55638) adult male 30day  (70823) adult female 01day  (135017) adult female 05day  (104138) adult female 30day  (115171) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    105761 embryo 02-04hr    155990 embryo 04-06hr    153970 embryo 06-08hr    170364 embryo 08-10hr    182390 embryo 10-12hr    212385 embryo 12-14hr    131258 embryo 14-16hr    172638 embryo 16-18hr    144228 embryo 18-20hr    167290 embryo 20-22hr    179162 embryo 22-24hr    141779 larva L1    220934 larva L2    160663 larva L3 12hr old    129084 larva L3 puffstage 1-2    108176 larva L3 puffstage 3-6    101593 larva L3 puffstage 7-9    97207 white prepupae new    93637 white prepupae 12hr    81795 white prepupae 24hr    78097 pupae 2d postWPP    76344 pupae 3d postWPP    62874 pupae 4d postWPP    75949 adult male 01day    51511 adult male 05day    55638 adult male 30day    70823 adult female 01day    135017 adult female 05day    104138 adult female 30day    115171 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0002626    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0002626 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: High levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male reproductive system, larval/adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0002626 expression level Tissue   Expression Level Larval Central Nervous System    2782.1 Larval Midgut    4208.3 Larval Hindgut    4353 Larval Malpighian Tubules    4894.7 Larval Fat Body    7041.1 Larval Salivary Gland    7986.2 Larval Trachea    6384.575 Larval Carcass    4698.35 Adult Head    4454.7 Adult Eye    6384.825 Adult Brain    2995.7 Adult Thoracic-Abdominal Ganglion    4151 Adult Crop    6162.8 Adult Midgut    4902.1 Adult Hindgut    4871.4 Adult Malpighian Tubules    2628.6 Adult Fat Body    5722.1 Adult Salivary Gland    11667.7 Adult Heart    7279.45 Adult VirginFemale Spermatheca    3194.5 Adult InseminatedFemale Spermatheca    3462.7 Adult Ovary    3298.1 Adult Testis    1031.4 Adult Male Accessory Gland    8599.5 Adult Carcass    5720.3 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  (2782.1) Larval Midgut  (4208.3) Larval Hindgut  (4353) Larval Malpighian Tubules  (4894.7) Larval Fat Body  (7041.1) Larval Salivary Gland  (7986.2) Larval Trachea  (6384.575) Larval Carcass  (4698.35) Adult Head  (4454.7) Adult Eye  (6384.825) Adult Brain  (2995.7) Adult Thoracic-Abdominal Ganglion  (4151) Adult Crop  (6162.8) Adult Midgut  (4902.1) Adult Hindgut  (4871.4) Adult Malpighian Tubules  (2628.6) Adult Fat Body  (5722.1) Adult Salivary Gland  (11667.7) Adult Heart  (7279.45) Adult VirginFemale Spermatheca  (3194.5) Adult InseminatedFemale Spermatheca  (3462.7) Adult Ovary  (3298.1) Adult Testis  (1031.4) Adult Male Accessory Gland  (8599.5) Adult Carcass  (5720.3) Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System  (2782.1) Larval Midgut  (4208.3) Larval Hindgut  (4353) Larval Malpighian Tubules  (4894.7) Larval Fat Body  (7041.1) Larval Salivary Gland  (7986.2) Larval Trachea  (6384.575) Larval Carcass  (4698.35) Adult Head  (4454.7) Adult Eye  (6384.825) Adult Brain  (2995.7) Adult Thoracic-Abdominal Ganglion  (4151) Adult Crop  (6162.8) Adult Midgut  (4902.1) Adult Hindgut  (4871.4) Adult Malpighian Tubules  (2628.6) Adult Fat Body  (5722.1) Adult Salivary Gland  (11667.7) Adult Heart  (7279.45) Adult VirginFemale Spermatheca  (3194.5) Adult InseminatedFemale Spermatheca  (3462.7) Adult Ovary  (3298.1) Adult Testis  1031.4 Adult Male Accessory Gland  (8599.5) Adult Carcass  (5720.3) Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    2782.1 Larval Midgut    4208.3 Larval Hindgut    4353 Larval Malpighian Tubules    4894.7 Larval Fat Body    7041.1 Larval Salivary Gland    7986.2 Larval Trachea    6384.575 Larval Carcass    4698.35 Adult Head    4454.7 Adult Eye    6384.825 Adult Brain    2995.7 Adult Thoracic-Abdominal Ganglion    4151 Adult Crop    6162.8 Adult Midgut    4902.1 Adult Hindgut    4871.4 Adult Malpighian Tubules    2628.6 Adult Fat Body    5722.1 Adult Salivary Gland    11667.7 Adult Heart    7279.45 Adult VirginFemale Spermatheca    3194.5 Adult InseminatedFemale Spermatheca    3462.7 Adult Ovary    3298.1 Adult Testis    1031.4 Adult Male Accessory Gland    8599.5 Adult Carcass    5720.3 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0002626 expression level Tissue   Expression Level Larval Central Nervous System    2782.1 Larval Midgut    4208.3 Larval Hindgut    4353 Larval Malpighian Tubules    4894.7 Larval Fat Body    7041.1 Larval Salivary Gland    7986.2 Larval Trachea    6384.575 Larval Carcass    4698.35 Adult Head    4454.7 Adult Eye    6384.825 Adult Brain    2995.7 Adult Thoracic-Abdominal Ganglion    4151 Adult Crop    6162.8 Adult Midgut    4902.1 Adult Hindgut    4871.4 Adult Malpighian Tubules    2628.6 Adult Fat Body    5722.1 Adult Salivary Gland    11667.7 Adult Heart    7279.45 Adult VirginFemale Spermatheca    3194.5 Adult InseminatedFemale Spermatheca    3462.7 Adult Ovary    3298.1 Adult Testis    1031.4 Adult Male Accessory Gland    8599.5 Adult Carcass    5720.3 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  (2782.1) Larval Midgut  (4208.3) Larval Hindgut  (4353) Larval Malpighian Tubules  (4894.7) Larval Fat Body  (7041.1) Larval Salivary Gland  (7986.2) Larval Trachea  (6384.575) Larval Carcass  (4698.35) Adult Head  (4454.7) Adult Eye  (6384.825) Adult Brain  (2995.7) Adult Thoracic-Abdominal Ganglion  (4151) Adult Crop  (6162.8) Adult Midgut  (4902.1) Adult Hindgut  (4871.4) Adult Malpighian Tubules  (2628.6) Adult Fat Body  (5722.1) Adult Salivary Gland  (11667.7) Adult Heart  (7279.45) Adult VirginFemale Spermatheca  (3194.5) Adult InseminatedFemale Spermatheca  (3462.7) Adult Ovary  (3298.1) Adult Testis  (1031.4) Adult Male Accessory Gland  (8599.5) Adult Carcass  (5720.3) Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System  (2782.1) Larval Midgut  (4208.3) Larval Hindgut  (4353) Larval Malpighian Tubules  (4894.7) Larval Fat Body  (7041.1) Larval Salivary Gland  (7986.2) Larval Trachea  (6384.575) Larval Carcass  (4698.35) Adult Head  (4454.7) Adult Eye  (6384.825) Adult Brain  (2995.7) Adult Thoracic-Abdominal Ganglion  (4151) Adult Crop  (6162.8) Adult Midgut  (4902.1) Adult Hindgut  (4871.4) Adult Malpighian Tubules  (2628.6) Adult Fat Body  (5722.1) Adult Salivary Gland  (11667.7) Adult Heart  (7279.45) Adult VirginFemale Spermatheca  (3194.5) Adult InseminatedFemale Spermatheca  (3462.7) Adult Ovary  (3298.1) Adult Testis  1031.4 Adult Male Accessory Gland  (8599.5) Adult Carcass  (5720.3) Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    2782.1 Larval Midgut    4208.3 Larval Hindgut    4353 Larval Malpighian Tubules    4894.7 Larval Fat Body    7041.1 Larval Salivary Gland    7986.2 Larval Trachea    6384.575 Larval Carcass    4698.35 Adult Head    4454.7 Adult Eye    6384.825 Adult Brain    2995.7 Adult Thoracic-Abdominal Ganglion    4151 Adult Crop    6162.8 Adult Midgut    4902.1 Adult Hindgut    4871.4 Adult Malpighian Tubules    2628.6 Adult Fat Body    5722.1 Adult Salivary Gland    11667.7 Adult Heart    7279.45 Adult VirginFemale Spermatheca    3194.5 Adult InseminatedFemale Spermatheca    3462.7 Adult Ovary    3298.1 Adult Testis    1031.4 Adult Male Accessory Gland    8599.5 Adult Carcass    5720.3 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    4454.7   NA  Eye    6384.825   NA  Brain    2995.7   2782.1  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    4151   NA  Crop    6162.8   4208.3  Midgut    4902.1   4353  Hindgut    4871.4   4894.7  Malpighian Tubules    2628.6   7041.1  Fat Body    5722.1   7986.2  Salivary Gland    11667.7   NA  Heart    7279.45   6384.575  Trachea    NA   NA  VirginFemale Spermatheca    3194.5   NA  InseminatedFemale Spermatheca    3462.7   NA  Ovary    3298.1   NA  Testis    1031.4   NA  Male Accessory Gland    8599.5   4698.35  Carcass    5720.3 modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_22
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0002626 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG7939-RB
Alleles & Phenotypes
Summary of Allele Phenotypes
Other Phenotypes Allele wild-type RpL32+t3.3 Phenotype manifest in Allele
Classical Alleles ( 0 )
For All Classical Alleles Show
Allele of RpL32 Class Mutagen Stocks Known lesion
Alleles Carried on Transgenic Constructs ( 5 )
For All Alleles Carried on Transgenic Constructs Show
Allele of RpL32 Class Mutagen Stocks Known lesion RpL32+t3.3 in vitro construct - genomic fragment 6 Yes RpL32KK100429 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes RpL32a.hs in vitro construct - regulatory fusion 0 Yes RpL32GD12323 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes RpL32hs.P in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3R)B81 (Kongsuwan et al., 1985, Kongsuwan et al., 1986) Df(3R)X3F (Cook, 2006.1.31, Schweisguth et al., 1990)
Not duplicated in	Dp(3;1)67A (Kongsuwan et al., 1985)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD12323} NA P{KK100429} NA heat-shock construct P{antisenseRpL32} NA P{M(3)99Ahs.P} NA characterization construct P{Jan/sry.CH8} NA P{RP49} NA
Insertions
	Type of insertions Name Expression data
Gene Ontology: Function, Process & Cellular Component ( 6 unique terms )
Terms Based on Experimental Evidence ( 2 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
mitotic spindle elongation inferred from mutant phenotype (Goshima et al., 2007) mitotic spindle organization inferred from mutant phenotype (Goshima et al., 2007)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 4 terms )
Molecular Function
CV term Evidence References
structural constituent of ribosome inferred from sequence or structural similarity with Saccharomyces RPL32 (FlyBase, 1992-) non-traceable author statement (Swiss-Prot Project Members, 1987.3.20)
Biological Process
CV term Evidence References
translation inferred from sequence or structural similarity with Saccharomyces RPL32 (FlyBase, 1992-)
Cellular Component
CV term Evidence References
cytosolic large ribosomal subunit inferred from sequence or structural similarity with Saccharomyces RPL32 (FlyBase, 1992-) ribosome non-traceable author statement (Swiss-Prot Project Members, 1987.3.20)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions RpL32 allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 68429 DroID - A comprehensive database of gene and protein interactions. • FBgn0002626 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 43573
Orthologs
Genome-wide drosophilid orthologs	Dana\GF23239 Dere\GG11969 Dgri\GH18400 Dmoj\GI23390 Dper\RpL32 Dpse\RpL32 Dsec\GM12186 Dsim\RpL32 Dvir\RpL32 Dwil\RpL32 Dyak\RpL32
Curated drosophilid orthologs	Daca\RpL32   Dafs\RpL32   Dazt\RpL32   Dbif\RpL32   Dbuz\RpL32   Dcap\RpL32   Dgua\RpL32   Dmad\RpL32   Dmir\RpL32   Dneb\RpL32   Dper\RpL32   Dpse\RpL32   Drep\RpL32   Dsal\RpL32   Dsim\RpL32 (Borie et al., 1999) Dstu\RpL32   Dsub\RpL32   Dsuc\RpL32   Dvir\RpL32   Dwil\RpL32   Dyak\RpL32
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AAEL003396-PA Aedes aegypti (mosquito) AGAP002122-PA Anopheles gambiae (mosquito) NP_001011587.1 Apis mellifera (honey bee) NP_001119682.1 Acyrthosiphon pisum (pea aphid) At5g46430.1 Arabidopsis thaliana (wall cress) 92154 Batrachochytrium dendrobatidis (chytrid fungus) 114866 Branchiostoma floridae (lancelet) 271557 Branchiostoma floridae (lancelet) XP_001897147.1 Brugia malayi (roundworm nematode) BGIBMGA000066-PA Bombyx mori (silk worm) ENSBTAP00000020323 Bos taurus (bovine) CAF0006985 Candida albicans (a form of yeast) CN24446 Caenorhabditis brenneri (small nematode) CBP06450 Caenorhabditis briggsae (nematode) CE03709 Caenorhabditis elegans (worm) ENSCAFP00000007278 Canis familiaris (dog) CAGL0H04521g Candida glabrata (haploid yeast) Chro.60387 Cryptosporidium hominis (obligate parasite) ENSCINP00000016542 Ciona intestinalis (sea squirt) JA10826 Caenorhabditis japonica (nematode) CMJ289C Cyanidioschyzon merolae (red alga) cn13213 Cryptococcus neoformans (yeast-like fungus) cgd6_3340 Cryptosporidium parvum (protozoan parasite) CPIJ001220 Culex pipens (mosquito) ENSCPOP00000019025 Cavia porcellus (guinea pig) 191758 Chlamidomonas reinhardtii (green alga) RP09590 Caenorhabditis remanei (nematode) ENSCSAVP00000016540 Ciona savignyi (sedentary tunicate) 154331 Capitella spl (polychaete worm) DDB0231150 Dictyostelium discoideum (amoeba) DEHA2F12430g Debaryomyces Hansenii (yeast) 230219 Daphnia pulex (daphnia) ENSDARP00000060004 Danio rerio (zebra fish) ENSECAP00000005318 Equus caballus (horse) XP_655599 Entamoeba histolytica (anaerobic protozoan) XP_656669 Entamoeba histolytica (anaerobic protozoan) XP_657219 Entamoeba histolytica (anaerobic protozoan) ENSGACP00000003538 Gymnopilus aculeatus (mushroom) GL50803_14049 Giardia lamblia (flagellated protozoan) 115955 Hypsipyla robusta (cedar shoot borer) ENSP00000380158 Homo sapiens (human) ISCW005648-PA Ixodes scapularis (deer tick) KLLA0E06843g Kluyveromyces lactis (yeast) LmjF21.1720 Leishmania major (virus) 33951 Monocosta brevicollis (choanoflagellate) ENSMODP00000007921 Monodelphis domestica (gray opossum) ENSMMUP00000001790 Macaca mulatta (rhesus monkey) ENSMMUP00000016442 Macaca mulatta (rhesus monkey) ENSMMUP00000024520 Macaca mulatta (rhesus monkey) ENSMUSP00000080523 Mus musculus (house mouse) 240738 Nematostella vectensis (starlet anemone) XP_001601579.1 Nasonia vitripennis (wasp) ENSOANP00000014093 Ornithorynchus anatinus (platypus) LOC_Os08g41300.1 Oryza sativa (asian rice) LOC_Os09g32500.1 Oryza sativa (asian rice) LOC_Os09g32520.1 Oryza sativa (asian rice) LOC_Os09g32532.1 Oryza sativa (asian rice) 23350 Ostreococcus tauri (green alga) PFI0190w Plasmodium falciparum (protozoal parasite) PHUM001709-PA Pediculus humanus (human body louse) PP13801 Pristionchus pacificus (nematode) 108328 Physcomitrella patens (moss) 165850 Physcomitrella patens (moss) 212907 Physcomitrella patens (moss) 228178 Physcomitrella patens (moss) ENSPPYP00000019087 Pongo pygmaeus (orangutan) 72106 Phytophthora ramorum (sudden oak death pathogen) 109525 Phytophthora sojae (root rot pathogen) 665064 Populus trichocarpa (black cottonwood) 711534 Populus trichocarpa (black cottonwood) 746779 Populus trichocarpa (black cottonwood) ENSPTRP00000025256 Pan troglodytes (chimpanzee) PVX_098640 Plasmodium vivax (protozoal parasite) ENSRNOP00000041638 Rattus norvegicus (norway rat) 147822 Sorghum bicolor (sorghum) 4748458 Sorghum bicolor (sorghum) 4891615 Sorghum bicolor (sorghum) YBL092W Saccharomyces cerevisiae (budding yeast) SPAC3H5.10 Schizosaccharomyces pombe (fission yeast) XP_001179121.1 Strongylocentrotus purpuratus (purple sea urchin) 36930 Trichoplax adhaerens (metazoa) TA08390 Theileria annulata (apicomplexan parasite) XP_969564.1 Tribolium castaneum (red flour beetle) Tc00.1047053504069.30 Trypanosoma cruzi (parasitic trypanosome) Tc00.1047053511145.20 Trypanosoma cruzi (parasitic trypanosome) ENSTNIP00000021051 Tetraodon nigroviridis (green spotted pufferfish) TP04_0094 Theileria parva (parasitic protozoan) 24410 Thalassiosira pseudonana (marine diatom) ENSTRUP00000023923 Takifugu rubripes (pufferfish Takifugu) 3836.m02032 Tetrahymena thermophila (ciliate protozoa) 89123.m00089 Trichomonas vaginalis (parasitic flagellated protozoan) 89123.m00093 Trichomonas vaginalis (parasitic flagellated protozoan) 90814.m00021 Trichomonas vaginalis (parasitic flagellated protozoan) ENSXETP00000003074 Xenopus tropicalis (frog) ENSXETP00000033825 Xenopus tropicalis (frog) YALI0E30811g Yarrowia lipolytica (nonconventional yeast) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB16401 NV15384 LH11997 ISCW005648 FBgn0068070 FBgn0068552 FBgn0016459 FBgn0040418 FBgn0167126 JGI_V11_230219 FBgn0012704 FBgn0018681 FBgn0146116 FBgn0125868 FBgn0104264 FBgn0100233 CPIJ001220 BGIBMGA000066 ACYPI000074 GB10903 AGAP002122 AAEL003396 PHUM499330 GLEAN_06106
Stocks & Reagents
Stocks Listed in FlyBase ( 7 )
Bloomington	2352 Df(3R)X3F, P{RP49}mtgP2 e1/TM3, Sb1 3546 Df(3R)B81, P{RP49}F2-80A e1/TM3, Sb1; Dp(3;1)67A
Kyoto	106779 Df(3R)X3F, P{RP49}mtgP2 e1/TM3, Sb1 107088 Df(3R)B81, P{RP49}F2-80A e1/TM3, Sb1; Dp(3;1)67A 101901 Df(3R)B81, P{RP49}F2-80A e1 / TM3, Sb1 ; Dp(3;1)67A 101625 Df(3R)X3F / P{RP49}mtgP2 e* / TM3, y+ kniri-1 pp vvlsep Sb1 Ubxbx-34e es
VDRC	v103396 P{KK100429}VIE-260B
Genomic Clones ( 2 )
	BACR05L15 BACR07A18 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 367 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	RE59709 RH03940
Other clones	IP17707 yak-ad_RpL32 yak-em_RpL32
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GH05333 GH06934 GH11463 GH12015 GH13961 GH20750 GH21646 GH23662 GH25175 GH27246 GM12857 GM12913 GM15446 GM26354 LD39671 LD47859 LP05575 LP07610 LP09048 LP11825 LP19636 LP21524 RE07109 RE09539 RE29083 RE47449 RE50124 RE50293 RE56701 RE58311 RE70473 RH02784 RH03437 RH04376 RH05870 RH05906 RH06606 RH07726 RH07942 RH08215 RH08520 RH11705 RH12109 RH12119 RH14896 RH18719 RH18752 RH18810 RH21904 RH22657 RH22691 RH23232 RH23604 RH23661 RH23715 RH24215 RH25195 RH25467 RH27404 RH27615 RH27722 RH27822 RH27885 RH28019 RH33694 RH34795 RH35147 RH35587 RH35589 RH36313 RH37022 RH38190 RH38471 RH39794 RH41690 RH42204 RH44111 RH44806 RH44973 RH45073 RH48246 RH48651 RH50273 RH50981 RH51193 RH52609 RH53476 RH54716 RH56112 RH59518 RH60108 RH61645 RH61811 RH61872 RH63979 RH65624 RH66503 RH66880 RH67386 RH67671 RH70220 SD11727 SD13847 SD16196 SD19703
Other clones	2172 7784 18973 34597 34970 35775 39231 43868 44559 44595 46123 52777 101961 102215 104632 104633 105149.1 155632 160172 162026 162162 173870 175769 176258 192391 193420 214243.30 280758 473679 522220 522501 522650 523113 524480 527231 527247 527888 528016 533882 542789 557769 557948 558178 562016 562168 569760 569798 572954 bs30a02 bs74e03 CK01.17A.B4 CK01.22D.G4 CK01.40D.F5 CK01.62B.G2 CK01.74C.D3 CK01.81D.E4 CK01.84C.F7 CK01.85C.G2 CK01.87D.F1 CK01.88C.C12 CK01.98D.G3 CK01.104B.D3 Dmel_dig_004387_0696_0884 Dmel_dig_004399_0642_1901 Dmel_dig_005202_0696_3329 Dmel_dig_006254_0700_3584 Dmel_dig_006990_0723_0606 Dmel_dig_007069_0690_1086 Dmel_dig_008975_0646_2431 Dmel_dig_010154_0624_1708 Dmel_dig_010270_0602_0676 Dmel_dig_010579_0630_1590 Dmel_dig_010592_0626_0457 Dmel_dig_011626_0604_0699 Dmel_dig_013930_0696_3191 Dmel_dig_014298_0666_3489 Dmel_dig_015552_0662_1458 Dmel_dig_017086_0665_1879 Dmel_dig_017453_0667_2267 Dmel_dig_017460_0662_3558 Dmel_dig_020335_0676_1736 Dmel_dig_021207_0591_0734 Dmel_neb_001054_0919_0318 Dmel_neb_006707_0186_2164 Dmel_neb_008841_0888_2760 Dmel_neb_008886_0642_2220 Dmel_neb_008950_0657_2837 Dmel_neb_009628_0874_3193 Dmel_neb_010536_0655_1492 Dmel_neb_010771_0658_2508 Dmel_neb_011728_0563_2564 Dmel_neb_012517_0591_2929 Dmel_neb_013610_0186_2223 Dmel_neb_013677_0682_1748 Dmel_neb_014795_0676_2904 Dmel_neb_014926_0348_2506 Dmel_neb_015002_0581_0521 Dmel_neb_015272_0883_2555 EC13725 EC14461 EC22084 EC22327 EC24537 EC24560 EC25359 EC27252 EC29342 EC30831 EC31477 EC35004 EC35653 EC37759 EC38694 EC38952 EC39253 EC39588 EC40413 EK005402 EK037930 EK050043 EK058514 EK064409 EK069321 EK087414 EK144858 EK145138 EK146715 EK165038 EK184329 EK258624 EK263044 EK265952 EK276323 EN00515 EN01564 EN02146 EN02512 EN03437 EN05457 EN07527 EN07545 EN08413 EN10340 EN10802 EN11331 EN12346 EN12716 EN13140 EN13839 EN15635 EN17558 EP04713 ESG011c.E7_B01 ESG011c.E21_B01 ESG0118c.B21_D11 ESG0119d.B21_F03 FGA007D07 FGA011C10 FGC008G10 FGC025B06 FGC032F06 FGH107H01 FGH119G01 FGM001A07 FGM226H06 FGM230G11 FGM230G12 GH06834 GH09713 GH13082 GH23116 IP10403 LD23557 LD33055 LD37345 LP01644 LP02315 LP06064 LP09161 LP11857 RE03292 RE05558 RE08451 RE11646 RE12186 RE19252 RE20782 RE22060 RE28337 RE28867 RE28868 RE29706 RE37465 RE39434 RE42431 RH01208 RH18440 RH31690 RH59177 RH66891 RP004048658 RP004054800 RP004064010 RP004135397 RP004138952 RP004139063 RP004151410 RP004154627 RP004156657 RP004157428 RP004162809 RP004168761 RP004179666 RP004182209 RP004182699 RP004196826 RP004200140 RP004201482 RP004202200 RP004203193 RP004204374 RP004206058 RP004211836 RP004219154 RP004224336 RP004227445 RP004239136 RP004244307 RP004249801 RP004254664 RP004254817 RP004259361 RP004260052 RP004288271 RP004297846 RP004321274 RP004323026 RP004361264 RP004383057 RP004390832 RP004407045 RP004419738 RP004446091 RP004472101 RP004495226 RP004504790 RP004509039 RP004513218 RP004513790 RP004524482 RP004531789 RP004536826 RP004541483 RP004543419 RP004546366 RP004549726 RP004556305
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0002626 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 43573
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: RpL32 BcDNA:RH03940 (FlyBase, 1996-)
Additional comments	Source for merge of RpL32 BcDNA:RH03940 was a shared cDNA (date:030728). (FlyBase, 1996-)
Other Comments
	RNAi screen using dsRNA made from templates generated with primers directed against this gene results in aberrantly short, monopolar spindles when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA. (Goshima et al., 2007) Minute gene. (Marygold et al., 2007) RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R+ cells. (Boutros et al., 2004) In a sample of 79 genes with multiple introns, 33 showed significant heterogeneity in G+C content among introns of the same gene and significant positive correspondence between the intron and the third codon position G+C content within genes. These results are consistent with selection adding against preferred codons at the start of genes. (Kliman and Eyre-Walker, 1998) Ecol\lacZDpt.PR adults are pricked with a sterile needle dipped in culture pellets of various living microorganisms (distinct bacterial strains, fungal spores or hyphae). Pricking results in a low but clearly detectable expression of all antimicrobial genes and these genes are induced above the background level by specific classes of microorganism. (Lemaitre et al., 1997) Chromosome homologies of Muller's element D (J chromosome in the Paleartic species and XR chromosome arm in Nearctic species) and of element E (O chromosome in the Paleartic species and 2 chromosome in Nearctic species) have been confirmed by single copy probes in the species of the obscura group and in D.melanogaster. (Segarra et al., 1996) Expression of transcript changes with adult age. (Shikama and Brack, 1996) The molecular organization of the 'rp49-Sry-jan' gene cluster, including gene order, direction of transcription and partial overlap of the janA and janB genes is strictly conserved between D.melanogaster and D.pseudoobscura.pseudoobscura. (Yanicostas et al., 1995) Ecdysteroid-regulated gene. (Andres et al., 1993) One of a class of genes that when present in one, rather than two, copies, produce a characteristic phenotype consisting of short slender bristles and delayed development. (Lindsley and Zimm, 1992) An antisense RpL32 gene driven by a strong and inducible promoter was integrated into the Drosophila germline. Induction of the gene caused a weak Minute phenotype and the transient arrest of oogenesis. (Patel and Jacobs-Lorena, 1992) Identification: Identified by the phenotype of heterozygotes for a synthetic deficiency. (Kongsuwan et al., 1986) Strong Minute; small bristles, slightly abnormal wings; eclosion delayed two to three days. (Kongsuwan et al., 1986) RNA blot analysis demonstrates that coordination between r-protein and rRNA synthesis is achieved by regulating translation of r-protein mRNAs in early embryos and by decreasing their abundance in adult tissues. (Al-Atia et al., 1985) RpL32/+ females produce smaller than normal eggs; 10-15% of embryos fail to hatch often displaying fusion and partial deletion of segments posterior to A3. (Kongsuwan et al., 1985)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_079843 NM_170460 NM_170461 NM_001144655 NM_001144656 RefSeq (Proteins) • NP_524582 NP_733339 NP_733340 NP_001138127 NP_001138128 DDBJ / EMBL / GenBank • AAB51389 AAF57001 AAL48127 AAN14210 AAN14211 ACL83583 ACL83584 AI297851 AI946722 AY070656 BI372677 BI590335 CAA25404 CAA74278 U92431 X00848 Y13939 Entrez Gene - A searchable database of RefSeq genes. • 43573 UniProtKB/Swiss-Prot • P04359
Other Crossreferences
	EPD - Eukarytoic Promoter Database, an annotated collection of POL II promoters • 07035 InterPro domains - A database of protein families, domains, and functional sites • Ribosomal protein L32e, conserved site (IPR018263) Ribosomal protein L32e (IPR001515)
Linkouts
	BioGRID - A database of protein and genetic interactions • 68429 DroID - A comprehensive database of gene and protein interactions. • FBgn0002626 DRSC - Results from RNAi screens. • FBgn0002626 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0002626 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG7939-RB FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0002626 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 43573 InParanoid A subset of ortholog calls from InParanoid. • AAEL003396-PA Aedes aegypti (mosquito) AGAP002122-PA Anopheles gambiae (mosquito) NP_001011587.1 Apis mellifera (honey bee) NP_001119682.1 Acyrthosiphon pisum (pea aphid) At5g46430.1 Arabidopsis thaliana (wall cress) 92154 Batrachochytrium dendrobatidis (chytrid fungus) 114866 Branchiostoma floridae (lancelet) 271557 Branchiostoma floridae (lancelet) XP_001897147.1 Brugia malayi (roundworm nematode) BGIBMGA000066-PA Bombyx mori (silk worm) ENSBTAP00000020323 Bos taurus (bovine) CAF0006985 Candida albicans (a form of yeast) CN24446 Caenorhabditis brenneri (small nematode) CBP06450 Caenorhabditis briggsae (nematode) CE03709 Caenorhabditis elegans (worm) ENSCAFP00000007278 Canis familiaris (dog) CAGL0H04521g Candida glabrata (haploid yeast) Chro.60387 Cryptosporidium hominis (obligate parasite) ENSCINP00000016542 Ciona intestinalis (sea squirt) JA10826 Caenorhabditis japonica (nematode) CMJ289C Cyanidioschyzon merolae (red alga) cn13213 Cryptococcus neoformans (yeast-like fungus) cgd6_3340 Cryptosporidium parvum (protozoan parasite) CPIJ001220 Culex pipens (mosquito) ENSCPOP00000019025 Cavia porcellus (guinea pig) 191758 Chlamidomonas reinhardtii (green alga) RP09590 Caenorhabditis remanei (nematode) ENSCSAVP00000016540 Ciona savignyi (sedentary tunicate) 154331 Capitella spl (polychaete worm) DDB0231150 Dictyostelium discoideum (amoeba) DEHA2F12430g Debaryomyces Hansenii (yeast) 230219 Daphnia pulex (daphnia) ENSDARP00000060004 Danio rerio (zebra fish) ENSECAP00000005318 Equus caballus (horse) XP_655599 Entamoeba histolytica (anaerobic protozoan) XP_656669 Entamoeba histolytica (anaerobic protozoan) XP_657219 Entamoeba histolytica (anaerobic protozoan) ENSGACP00000003538 Gymnopilus aculeatus (mushroom) GL50803_14049 Giardia lamblia (flagellated protozoan) 115955 Hypsipyla robusta (cedar shoot borer) ENSP00000380158 Homo sapiens (human) ISCW005648-PA Ixodes scapularis (deer tick) KLLA0E06843g Kluyveromyces lactis (yeast) LmjF21.1720 Leishmania major (virus) 33951 Monocosta brevicollis (choanoflagellate) ENSMODP00000007921 Monodelphis domestica (gray opossum) ENSMMUP00000001790 Macaca mulatta (rhesus monkey) ENSMMUP00000016442 Macaca mulatta (rhesus monkey) ENSMMUP00000024520 Macaca mulatta (rhesus monkey) ENSMUSP00000080523 Mus musculus (house mouse) 240738 Nematostella vectensis (starlet anemone) XP_001601579.1 Nasonia vitripennis (wasp) ENSOANP00000014093 Ornithorynchus anatinus (platypus) LOC_Os08g41300.1 Oryza sativa (asian rice) LOC_Os09g32500.1 Oryza sativa (asian rice) LOC_Os09g32520.1 Oryza sativa (asian rice) LOC_Os09g32532.1 Oryza sativa (asian rice) 23350 Ostreococcus tauri (green alga) PFI0190w Plasmodium falciparum (protozoal parasite) PHUM001709-PA Pediculus humanus (human body louse) PP13801 Pristionchus pacificus (nematode) 108328 Physcomitrella patens (moss) 165850 Physcomitrella patens (moss) 212907 Physcomitrella patens (moss) 228178 Physcomitrella patens (moss) ENSPPYP00000019087 Pongo pygmaeus (orangutan) 72106 Phytophthora ramorum (sudden oak death pathogen) 109525 Phytophthora sojae (root rot pathogen) 665064 Populus trichocarpa (black cottonwood) 711534 Populus trichocarpa (black cottonwood) 746779 Populus trichocarpa (black cottonwood) ENSPTRP00000025256 Pan troglodytes (chimpanzee) PVX_098640 Plasmodium vivax (protozoal parasite) ENSRNOP00000041638 Rattus norvegicus (norway rat) 147822 Sorghum bicolor (sorghum) 4748458 Sorghum bicolor (sorghum) 4891615 Sorghum bicolor (sorghum) YBL092W Saccharomyces cerevisiae (budding yeast) SPAC3H5.10 Schizosaccharomyces pombe (fission yeast) XP_001179121.1 Strongylocentrotus purpuratus (purple sea urchin) 36930 Trichoplax adhaerens (metazoa) TA08390 Theileria annulata (apicomplexan parasite) XP_969564.1 Tribolium castaneum (red flour beetle) Tc00.1047053504069.30 Trypanosoma cruzi (parasitic trypanosome) Tc00.1047053511145.20 Trypanosoma cruzi (parasitic trypanosome) ENSTNIP00000021051 Tetraodon nigroviridis (green spotted pufferfish) TP04_0094 Theileria parva (parasitic protozoan) 24410 Thalassiosira pseudonana (marine diatom) ENSTRUP00000023923 Takifugu rubripes (pufferfish Takifugu) 3836.m02032 Tetrahymena thermophila (ciliate protozoa) 89123.m00089 Trichomonas vaginalis (parasitic flagellated protozoan) 89123.m00093 Trichomonas vaginalis (parasitic flagellated protozoan) 90814.m00021 Trichomonas vaginalis (parasitic flagellated protozoan) ENSXETP00000003074 Xenopus tropicalis (frog) ENSXETP00000033825 Xenopus tropicalis (frog) YALI0E30811g Yarrowia lipolytica (nonconventional yeast) InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 43573 modMine - Data generated by the modENCODE project. • FBgn0002626 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB16401 NV15384 LH11997 ISCW005648 FBgn0068070 FBgn0068552 FBgn0016459 FBgn0040418 FBgn0167126 JGI_V11_230219 FBgn0012704 FBgn0018681 FBgn0146116 FBgn0125868 FBgn0104264 FBgn0100233 CPIJ001220 BGIBMGA000066 ACYPI000074 GB10903 AGAP002122 AAEL003396 PHUM499330 GLEAN_06106
Synonyms & Secondary IDs ( 21 )
Reported As
Symbol Synonym	143250_at (Kristensen et al., 2005) BcDNA:RH03940   bs30a02.y1 (Andrews et al., 2000) CG7939 (Goshima et al., 2007, Keleman et al., 2009.8.5, Marygold et al., 2007, Li et al., 2007, Willis et al., 2010, Ling and Salvaterra, 2011, Ponton et al., 2011, Cammarato et al., 2011) L32 (Brogna et al., 2002) M(3)99D (Cook, 2006.1.31, Kliman and Eyre-Walker, 1998, Ruiz et al., 1997, Moriyama and Powell, 1997, Marygold et al., 2007) Rp-49 (Pal Bhadra et al., 2005) RP-49 (Bondinas et al., 2002, Bondinas et al., 2001) rp49 (Herold et al., 2003, Beltran et al., 2003, Lee et al., 2003, Fujii and Amrein, 2002, Juge et al., 2002, Lee et al., 2002, Tulin et al., 2002, Herold et al., 2001, Rozas et al., 2001, Frolov et al., 2000, Clark et al., 2000, Bernal and Kimbrell, 2000, D'Avino and Thummel, 2000, Basset et al., 2000, Koushika et al., 2000, Reim et al., 1999, Rozas, 1997.6.23, McMahill, 1997.3.6, Borie et al., 1999, Lam et al., 1999, Hirose et al., 1999, Frolov and Birchler, 1998, Kopczynski et al., 1998, Munte et al., 1997, Segarra et al., 1996, Shikama and Brack, 1996, Comeron, 1995, Baehrecke and Thummel, 1995, Myers et al., 1995, Yanicostas et al., 1995, Ferrer et al., 1994, Andres et al., 1993, Segarra and Aguade, 1993, Carulli et al., 1993, Huet et al., 1993, Crozatier et al., 1992, Moriyama and Gojobori, 1992, Patel and Jacobs-Lorena, 1992, Hongo and Jacobs-Lorena, 1991, Tamate et al., 1990, Schweisguth et al., 1990, Polito et al., 1990, Jacks et al., 1988, Vincent et al., 1985, Al-Atia et al., 1985, Vincent et al., 1984, Fic et al., 2007, Robida et al., 2007, Lu et al., 2009, Kurapati et al., 2004, Krauss et al., 2009, Besse et al., 2009, Farkas and Mechler, 2000) Rp49 (Paul and Duttaroy, 2003, Bhadra et al., 2000, Civetta and Singh, 1998, Lemaitre et al., 1997, Maroni, 1994, Lozovskaya et al., 1993, Furuhashi et al., 2006, Kwong et al., 2008, Ida et al., 2009, Zhou et al., 2008, Sakai et al., 2009, Bartkowiak et al., 2010) RP49 (Brody et al., 2002, Yanicostas et al., 1989, Lee et al., 2010, Li et al., 2007) Rp49/RpL32 (Comeron and Kreitman, 1998) rp49/RpL32 (Willis et al., 2010) RpL32 (Wang et al., 2004, Earl and Britt, 2006, Bartolome and Charlesworth, 2006, Goshima et al., 2007, Walser et al., 2006, Fanti et al., 2008, Nie et al., 2009, Marygold et al., 2007, Gim et al., 2001, Zsindely et al., 2009, Ling and Salvaterra, 2011, Meiklejohn et al., 2011) Rpl32 (Bossuyt et al., 2009, Bartkuhn et al., 2009, Chintapalli et al., 2007, Ponton et al., 2011, D'Hulst et al., 2006) RPL32 (Pyke et al., 2004)
Name Synonym	Minute(3)99D   Ribosomal protein 49/L32 (Ling and Salvaterra, 2011) Ribosomal protein L32 (Gim et al., 2001, Cammarato et al., 2011) ribosomal protein L32 (Zsindely et al., 2009) r-protein 49 (Hongo and Jacobs-Lorena, 1991)
Secondary FlyBase IDs
	FBgn0010264 FBgn0047051
References ( 137 )
Generate a list of	All references relating to this gene ( 137 )
List References by type	Research paper  ( 116 ) Supplementary material  ( 7 ) Review  ( 1 ) Personal communication to FlyBase  ( 4 ) Abstract  ( 1 ) FlyBase analysis  ( 2 ) DNA/RNA sequence record  ( 2 ) Book  ( 1 ) Protein sequence record  ( 1 ) Computer file  ( 1 ) Automatic genome annotation  ( 1 )
Recent research papers ( 8 )
	Booker et al., 2011, BMC Genomics 12: 50 False negative rates in Drosophila cell-based RNAi screens: a case study. [FBrf0212969] Goda et al., 2011, PLoS Genet. 7(7): e1002167 Adult Circadian Behavior in Drosophila Requires Developmental Expression of cycle, But Not period. [FBrf0214302] Ling and Salvaterra, 2011, PLoS ONE 6(3): e17762 Robust RT-qPCR Data Normalization: Validation and Selection of Internal Reference Genes during Post-Experimental Data Analysis. [FBrf0213272] Meiklejohn et al., 2011, PLoS Biol. 9(8): e1001126 Sex Chromosome-Specific Regulation in the Drosophila Male Germline But Little Evidence for Chromosomal Dosage Compensation or Meiotic Inactivation. [FBrf0214756] Ponton et al., 2011, J. Insect Physiol. 57(6): 840--850 Evaluation of potential reference genes for reverse transcription-qPCR studies of physiological responses in Drosophila melanogaster. [FBrf0213828] Bartkowiak et al., 2010, Genes Dev. 24(20): 2303--2316 CDK12 is a transcription elongation-associated CTD kinase, the metazoan ortholog of yeast Ctk1. [FBrf0212082] Lee et al., 2010, Mol. Cell. Biol. 30(10): 2563--2577 Drosophila RB proteins repress differentiation-specific genes via two different mechanisms. [FBrf0210656] Willis et al., 2010, J. Insect Sci. 10: 66 Microarray Analysis of Juvenile Hormone Response in Drosophila melanogaster S2 cells. [FBrf0211402] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...