rp49, M(3)99D, RP-49, L32, rp49/RpL32
Annotated transcripts do not represent all supported alternative splices within 5' UTR.
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.47
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\RpL32 using the Feature Mapper tool.
GBrowse - Visual display of RNA-Seq signalsView Dmel\RpL32 in GBrowse 2
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
RNAi screen using dsRNA made from templates generated with primers directed against this gene results in aberrantly short, monopolar spindles when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.
RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R+ cells.
In a sample of 79 genes with multiple introns, 33 showed significant heterogeneity in G+C content among introns of the same gene and significant positive correspondence between the intron and the third codon position G+C content within genes. These results are consistent with selection adding against preferred codons at the start of genes.
Ecol\lacZDpt.PR adults are pricked with a sterile needle dipped in culture pellets of various living microorganisms (distinct bacterial strains, fungal spores or hyphae). Pricking results in a low but clearly detectable expression of all antimicrobial genes and these genes are induced above the background level by specific classes of microorganism.
Chromosome homologies of Muller's element D (J chromosome in the Paleartic species and XR chromosome arm in Nearctic species) and of element E (O chromosome in the Paleartic species and 2 chromosome in Nearctic species) have been confirmed by single copy probes in the species of the obscura group and in D.melanogaster.
Expression of transcript changes with adult age.
The molecular organization of the 'rp49-Sry-jan' gene cluster, including gene order, direction of transcription and partial overlap of the janA and janB genes is strictly conserved between D.melanogaster and D.pseudoobscura.pseudoobscura.
One of a class of genes that when present in one, rather than two, copies, produce a characteristic phenotype consisting of short slender bristles and delayed development.
An antisense RpL32 gene driven by a strong and inducible promoter was integrated into the Drosophila germline. Induction of the gene caused a weak Minute phenotype and the transient arrest of oogenesis.
Identification: Identified by the phenotype of heterozygotes for a synthetic deficiency.
Strong Minute; small bristles, slightly abnormal wings; eclosion delayed two to three days.
RNA blot analysis demonstrates that coordination between r-protein and rRNA synthesis is achieved by regulating translation of r-protein mRNAs in early embryos and by decreasing their abundance in adult tissues.