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Gene Dmel\HLHm7

General Information
Symbol	Dmel\HLHm7	Species	D. melanogaster
Name	E(spl) region transcript m7	Annotation symbol	CG8361
Feature type	protein_coding_gene	FlyBase ID	FBgn0002633
Gene Model Status	Current	Stock availability	9 publicly available
Also Known As	m7, E(spl)m7, E(spl)-m7, HLH-m7
Genomic Location
Chromosome (arm)	3R	Recombination map	3-89.1
Cytogenetic map	96F10-96F10	Sequence location	3R:21,862,760..21,863,741 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene E(spl) region transcript m7 is referred to in FlyBase by the symbol Dmel\HLHm7 (CG8361, FBgn0002633). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription; transcription factor binding; sequence-specific DNA binding. There is experimental evidence that it is involved in the biological process: startle response; brain morphogenesis; dendrite morphogenesis; neuron development; negative regulation of transcription from RNA polymerase II promoter; locomotion involved in locomotory behavior. 25 alleles are reported. The phenotypes of these alleles are annotated with: organ system subdivision; adult segment; adult mesothoracic segment; sensillum; external compound sense organ; compound cell cluster organ; portion of tissue; integumentary plate; somatic cell; thoracic segment; multi-cell-component structure; organ system. It has one annotated transcript and one annotated polypeptide. Protein features are: Helix-loop-helix DNA-binding; Orange; Orange subgroup. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of extremely low expression. Peak expression observed within 06-12 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system. Comments on Affy2 ProbeSet: ProbeSet 1625493_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of HLHm7. Gene sequence location is 3R:21862760..21863741.
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2011_10
FB2012_01	References Mulligan et al., 2011, Mol. Cell 42(5): 689--699 Lieber et al., 2011, Neuron 69(3): 468--481 Sequence features BKN30898 HFA16696 Controlled Vocabulary Terms transcription factor binding
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 96F10-96F10   Limits computationally determined from genome sequence between P{PZ}l(3)rQ197rQ197 and P{lacW}scribj7B3
Experimentally Determined Cytological Location
Cytogenetic map Notes References 96F11-96F14     (Posakony, 1994.8.5)
Experimentally Determined Recombination Data
Location	3-89.1
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\HLHm7 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.42
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) HLHm7-RA FBtr0084960  NM_080505   982   186
Additional Transcript Data & Comments
Reported size (kB)	1.5 (northern blot) (Knust et al., 1987)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein HLHm7-PA   FBpp0084334   20.7   186   9.63     NP_536753   AAF56554
Additional Polypeptide Data & Comments
Reported size (kDa)	186 (aa); 20.5 (kD predicted) (Klambt et al., 1989)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Orange subgroup (IPR018352) Orange (IPR003650) Helix-loop-helix DNA-binding (IPR011598)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE014297 AAF56554   AI124338   AQ073039   AY779906 AAV59050   AY779907 AAV59062   AY779908 AAV59074   AY779909 AAV59086   AY779910 AAV59098   AY779911 AAV59110   AY779912 AAV59122   AY779913 AAV59134   AY779914 AAV59146   AY779915 AAV59158   AY779916 AAV59170   AY779917 AAV59182   AY779918 AAV59194   AY779919 AAV59206   AY779920 AAV59218   AY779921 AAV59230   BT022121   CO262619   CO304812   CO314768   EC065749   EC075960   EC194729   EC207915   EC214231   EL878983   EL879182   U13069   X16553 CAA34553
UniProtKB/Swiss-Prot	P13097
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References protein binding site FBsf0000160034 3R:21,862,530..21,862,535 bound_moiety=da/da, da/ac, da/sc proneural protein complexes comment=Bound by da/ac, da/sc proneural protein complexes and weakly by da/da homodimers. evidence=experimental (Posakony, 1994.8.5) protein binding site FBsf0000160035 3R:21,862,628..21,862,633 bound_moiety=da/da, da/ac, da/sc proneural protein complexes comment=Bound by da/ac, da/sc proneural protein complexes and weakly by da/da homodimers. evidence=experimental (Posakony, 1994.8.5)
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 5   organism | striped   (Knust et al., 1987) mesectoderm   (Rao et al., 1991) embryonic stage 6 -- 8   mesectoderm   (Wech et al., 1999) amnioserosa anlage   (Wech et al., 1999) embryonic stage 8 -- 11   ventral neurogenic region   (Wech et al., 1999) proneural cluster   (Wech et al., 1999) embryonic stage 9   endoderm   (Wech et al., 1999) Comment:transiently expressed embryonic stage 10   posterior midgut primordium   (Knust et al., 1987) supraesophageal ganglion   (Knust et al., 1987) embryonic stage 11   mesoderm   (Wech et al., 1999) embryonic stage 11 -- 14   embryonic optic lobe primordium   (Knust et al., 1987) stomatogastric nervous system primordium   (Knust et al., 1987) embryonic stage 12 -- 15   embryonic head segment   (Wech et al., 1999) embryonic thoracic segment   (Wech et al., 1999) northern blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage 5   vitellophage   (Knust et al., 1987) embryonic stage 7 -- 11   ectoderm   (Knust et al., 1987) embryonic stage 11   epidermis | restricted   (Knust et al., 1987) Comment:late stage 11 embryonic stage 11 -- 12   mesoderm   (Knust et al., 1987) embryonic stage 11 -- 13     (Knust et al., 1987) Comment:reference states 6-10 hr AEL
Additional Descriptive Data
	The peak of HLHm7 expression during embryogenesis occurs at 6-10 hours. In the late blastoderm, expression is detected in a 2-3 cell-wide stripe on each side of the embryo, in groups of cells over the dorsal half of the poles, in the vitellophage, and in a dorsomedian band spanning the anterioposterior axis. During germ band extension, ectodermal expression is detected, and at the extended germ band stage, epidermal expression is abundant. In late stage 11, epidermal expression becomes patchy. At stage 10, the primordia of the supraoesophageal ganglion and the posterior midgut express HLHm7. From stage 11 through late stage 12, expression is detected in the entire mesodermal layer. From late stage 11 through stage 14, expression is also detected in the primordia of the stomatogastric nervous system and in the optic lobes. (Knust et al., 1987)
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0002633    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0002633 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of extremely low expression. Peak expression observed within 06-12 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0002633 expression level Developmental Stage   Expression Level embryo 00-02hr    209 embryo 02-04hr    2177 embryo 04-06hr    8881 embryo 06-08hr    16197 embryo 08-10hr    15716 embryo 10-12hr    6846 embryo 12-14hr    4061 embryo 14-16hr    1319 embryo 16-18hr    773 embryo 18-20hr    454 embryo 20-22hr    136 embryo 22-24hr    178 larva L1    19 larva L2    10 larva L3 12hr old    25 larva L3 puffstage 1-2    296 larva L3 puffstage 3-6    630 larva L3 puffstage 7-9    992 white prepupae new    1135 white prepupae 12hr    2117 white prepupae 24hr    1516 pupae 2d postWPP    470 pupae 3d postWPP    77 pupae 4d postWPP    101 adult male 01day    18 adult male 05day    9 adult male 30day    6 adult female 01day    59 adult female 05day    33 adult female 30day    29 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (209) embryo 02-04hr  (2177) embryo 04-06hr  (8881) embryo 06-08hr  (16197) embryo 08-10hr  (15716) embryo 10-12hr  (6846) embryo 12-14hr  (4061) embryo 14-16hr  (1319) embryo 16-18hr  (773) embryo 18-20hr  (454) embryo 20-22hr  (136) embryo 22-24hr  (178) larva L1    19 larva L2    10 larva L3 12hr old    25 larva L3 puffstage 1-2  (296) larva L3 puffstage 3-6  (630) larva L3 puffstage 7-9  (992) white prepupae new  (1135) white prepupae 12hr  (2117) white prepupae 24hr  (1516) pupae 2d postWPP  (470) pupae 3d postWPP    77 pupae 4d postWPP  101 adult male 01day    18 adult male 05day    9 adult male 30day    6 adult female 01day    59 adult female 05day    33 adult female 30day    29 Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    209 embryo 02-04hr  (2177) embryo 04-06hr  (8881) embryo 06-08hr  (16197) embryo 08-10hr  (15716) embryo 10-12hr  (6846) embryo 12-14hr  (4061) embryo 14-16hr    1319 embryo 16-18hr    773 embryo 18-20hr    454 embryo 20-22hr    136 embryo 22-24hr    178 larva L1    19 larva L2    10 larva L3 12hr old    25 larva L3 puffstage 1-2    296 larva L3 puffstage 3-6    630 larva L3 puffstage 7-9    992 white prepupae new    1135 white prepupae 12hr  (2117) white prepupae 24hr  (1516) pupae 2d postWPP    470 pupae 3d postWPP    77 pupae 4d postWPP    101 adult male 01day    18 adult male 05day    9 adult male 30day    6 adult female 01day    59 adult female 05day    33 adult female 30day    29 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    209 embryo 02-04hr    2177 embryo 04-06hr    8881 embryo 06-08hr  (16197) embryo 08-10hr  (15716) embryo 10-12hr    6846 embryo 12-14hr    4061 embryo 14-16hr    1319 embryo 16-18hr    773 embryo 18-20hr    454 embryo 20-22hr    136 embryo 22-24hr    178 larva L1    19 larva L2    10 larva L3 12hr old    25 larva L3 puffstage 1-2    296 larva L3 puffstage 3-6    630 larva L3 puffstage 7-9    992 white prepupae new    1135 white prepupae 12hr    2117 white prepupae 24hr    1516 pupae 2d postWPP    470 pupae 3d postWPP    77 pupae 4d postWPP    101 adult male 01day    18 adult male 05day    9 adult male 30day    6 adult female 01day    59 adult female 05day    33 adult female 30day    29 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    209 embryo 02-04hr    2177 embryo 04-06hr    8881 embryo 06-08hr    16197 embryo 08-10hr    15716 embryo 10-12hr    6846 embryo 12-14hr    4061 embryo 14-16hr    1319 embryo 16-18hr    773 embryo 18-20hr    454 embryo 20-22hr    136 embryo 22-24hr    178 larva L1    19 larva L2    10 larva L3 12hr old    25 larva L3 puffstage 1-2    296 larva L3 puffstage 3-6    630 larva L3 puffstage 7-9    992 white prepupae new    1135 white prepupae 12hr    2117 white prepupae 24hr    1516 pupae 2d postWPP    470 pupae 3d postWPP    77 pupae 4d postWPP    101 adult male 01day    18 adult male 05day    9 adult male 30day    6 adult female 01day    59 adult female 05day    33 adult female 30day    29 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0002633 expression level Developmental Stage   Expression Level embryo 00-02hr    209 embryo 02-04hr    2177 embryo 04-06hr    8881 embryo 06-08hr    16197 embryo 08-10hr    15716 embryo 10-12hr    6846 embryo 12-14hr    4061 embryo 14-16hr    1319 embryo 16-18hr    773 embryo 18-20hr    454 embryo 20-22hr    136 embryo 22-24hr    178 larva L1    19 larva L2    10 larva L3 12hr old    25 larva L3 puffstage 1-2    296 larva L3 puffstage 3-6    630 larva L3 puffstage 7-9    992 white prepupae new    1135 white prepupae 12hr    2117 white prepupae 24hr    1516 pupae 2d postWPP    470 pupae 3d postWPP    77 pupae 4d postWPP    101 adult male 01day    18 adult male 05day    9 adult male 30day    6 adult female 01day    59 adult female 05day    33 adult female 30day    29 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (209) embryo 02-04hr  (2177) embryo 04-06hr  (8881) embryo 06-08hr  (16197) embryo 08-10hr  (15716) embryo 10-12hr  (6846) embryo 12-14hr  (4061) embryo 14-16hr  (1319) embryo 16-18hr  (773) embryo 18-20hr  (454) embryo 20-22hr  136 embryo 22-24hr  (178) larva L1    19 larva L2    10 larva L3 12hr old    25 larva L3 puffstage 1-2  (296) larva L3 puffstage 3-6  (630) larva L3 puffstage 7-9  (992) white prepupae new  (1135) white prepupae 12hr  (2117) white prepupae 24hr  (1516) pupae 2d postWPP  (470) pupae 3d postWPP    77 pupae 4d postWPP  101 adult male 01day    18 adult male 05day    9 adult male 30day    6 adult female 01day    59 adult female 05day    33 adult female 30day    29 Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    209 embryo 02-04hr  2177 embryo 04-06hr  (8881) embryo 06-08hr  (16197) embryo 08-10hr  (15716) embryo 10-12hr  (6846) embryo 12-14hr  (4061) embryo 14-16hr    1319 embryo 16-18hr    773 embryo 18-20hr    454 embryo 20-22hr    136 embryo 22-24hr    178 larva L1    19 larva L2    10 larva L3 12hr old    25 larva L3 puffstage 1-2    296 larva L3 puffstage 3-6    630 larva L3 puffstage 7-9    992 white prepupae new    1135 white prepupae 12hr  2117 white prepupae 24hr  1516 pupae 2d postWPP    470 pupae 3d postWPP    77 pupae 4d postWPP    101 adult male 01day    18 adult male 05day    9 adult male 30day    6 adult female 01day    59 adult female 05day    33 adult female 30day    29 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    209 embryo 02-04hr    2177 embryo 04-06hr    8881 embryo 06-08hr  16197 embryo 08-10hr  15716 embryo 10-12hr    6846 embryo 12-14hr    4061 embryo 14-16hr    1319 embryo 16-18hr    773 embryo 18-20hr    454 embryo 20-22hr    136 embryo 22-24hr    178 larva L1    19 larva L2    10 larva L3 12hr old    25 larva L3 puffstage 1-2    296 larva L3 puffstage 3-6    630 larva L3 puffstage 7-9    992 white prepupae new    1135 white prepupae 12hr    2117 white prepupae 24hr    1516 pupae 2d postWPP    470 pupae 3d postWPP    77 pupae 4d postWPP    101 adult male 01day    18 adult male 05day    9 adult male 30day    6 adult female 01day    59 adult female 05day    33 adult female 30day    29 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    209 embryo 02-04hr    2177 embryo 04-06hr    8881 embryo 06-08hr    16197 embryo 08-10hr    15716 embryo 10-12hr    6846 embryo 12-14hr    4061 embryo 14-16hr    1319 embryo 16-18hr    773 embryo 18-20hr    454 embryo 20-22hr    136 embryo 22-24hr    178 larva L1    19 larva L2    10 larva L3 12hr old    25 larva L3 puffstage 1-2    296 larva L3 puffstage 3-6    630 larva L3 puffstage 7-9    992 white prepupae new    1135 white prepupae 12hr    2117 white prepupae 24hr    1516 pupae 2d postWPP    470 pupae 3d postWPP    77 pupae 4d postWPP    101 adult male 01day    18 adult male 05day    9 adult male 30day    6 adult female 01day    59 adult female 05day    33 adult female 30day    29 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0002633    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0002633 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0002633 expression level Tissue   Expression Level Larval Central Nervous System    136.575 Larval Midgut    4.4 Larval Hindgut    6.4 Larval Malpighian Tubules    6.9 Larval Fat Body no informative data Larval Salivary Gland    5.1 Larval Trachea no informative data Larval Carcass no informative data Adult Head    3.6 Adult Eye    7.925 Adult Brain    6.2 Adult Thoracic-Abdominal Ganglion    4.3 Adult Crop    3.7 Adult Midgut    3.1 Adult Hindgut    3.9 Adult Malpighian Tubules    4.5 Adult Fat Body    5 Adult Salivary Gland    4.9 Adult Heart    3.25 Adult VirginFemale Spermatheca    3.9 Adult InseminatedFemale Spermatheca    3.2 Adult Ovary    4.6 Adult Testis    3.6 Adult Male Accessory Gland    2.4 Adult Carcass    4.1 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    136.575 Larval Midgut    4.4 Larval Hindgut    6.4 Larval Malpighian Tubules    6.9 Larval Fat Body no informative data Larval Salivary Gland    5.1 Larval Trachea no informative data Larval Carcass no informative data Adult Head    3.6 Adult Eye    7.925 Adult Brain    6.2 Adult Thoracic-Abdominal Ganglion    4.3 Adult Crop    3.7 Adult Midgut    3.1 Adult Hindgut    3.9 Adult Malpighian Tubules    4.5 Adult Fat Body    5 Adult Salivary Gland    4.9 Adult Heart    3.25 Adult VirginFemale Spermatheca    3.9 Adult InseminatedFemale Spermatheca    3.2 Adult Ovary    4.6 Adult Testis    3.6 Adult Male Accessory Gland    2.4 Adult Carcass    4.1 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    136.575 Larval Midgut    4.4 Larval Hindgut    6.4 Larval Malpighian Tubules    6.9 Larval Fat Body no informative data Larval Salivary Gland    5.1 Larval Trachea no informative data Larval Carcass no informative data Adult Head    3.6 Adult Eye    7.925 Adult Brain    6.2 Adult Thoracic-Abdominal Ganglion    4.3 Adult Crop    3.7 Adult Midgut    3.1 Adult Hindgut    3.9 Adult Malpighian Tubules    4.5 Adult Fat Body    5 Adult Salivary Gland    4.9 Adult Heart    3.25 Adult VirginFemale Spermatheca    3.9 Adult InseminatedFemale Spermatheca    3.2 Adult Ovary    4.6 Adult Testis    3.6 Adult Male Accessory Gland    2.4 Adult Carcass    4.1 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    136.575 Larval Midgut    4.4 Larval Hindgut    6.4 Larval Malpighian Tubules    6.9 Larval Fat Body no informative data Larval Salivary Gland    5.1 Larval Trachea no informative data Larval Carcass no informative data Adult Head    3.6 Adult Eye    7.925 Adult Brain    6.2 Adult Thoracic-Abdominal Ganglion    4.3 Adult Crop    3.7 Adult Midgut    3.1 Adult Hindgut    3.9 Adult Malpighian Tubules    4.5 Adult Fat Body    5 Adult Salivary Gland    4.9 Adult Heart    3.25 Adult VirginFemale Spermatheca    3.9 Adult InseminatedFemale Spermatheca    3.2 Adult Ovary    4.6 Adult Testis    3.6 Adult Male Accessory Gland    2.4 Adult Carcass    4.1 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0002633 expression level Tissue   Expression Level Larval Central Nervous System    136.575 Larval Midgut    4.4 Larval Hindgut    6.4 Larval Malpighian Tubules    6.9 Larval Fat Body no informative data Larval Salivary Gland    5.1 Larval Trachea no informative data Larval Carcass no informative data Adult Head    3.6 Adult Eye    7.925 Adult Brain    6.2 Adult Thoracic-Abdominal Ganglion    4.3 Adult Crop    3.7 Adult Midgut    3.1 Adult Hindgut    3.9 Adult Malpighian Tubules    4.5 Adult Fat Body    5 Adult Salivary Gland    4.9 Adult Heart    3.25 Adult VirginFemale Spermatheca    3.9 Adult InseminatedFemale Spermatheca    3.2 Adult Ovary    4.6 Adult Testis    3.6 Adult Male Accessory Gland    2.4 Adult Carcass    4.1 Expression Level Scale  None   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    136.575 Larval Midgut    4.4 Larval Hindgut    6.4 Larval Malpighian Tubules    6.9 Larval Fat Body no informative data Larval Salivary Gland    5.1 Larval Trachea no informative data Larval Carcass no informative data Adult Head    3.6 Adult Eye    7.925 Adult Brain    6.2 Adult Thoracic-Abdominal Ganglion    4.3 Adult Crop    3.7 Adult Midgut    3.1 Adult Hindgut    3.9 Adult Malpighian Tubules    4.5 Adult Fat Body    5 Adult Salivary Gland    4.9 Adult Heart    3.25 Adult VirginFemale Spermatheca    3.9 Adult InseminatedFemale Spermatheca    3.2 Adult Ovary    4.6 Adult Testis    3.6 Adult Male Accessory Gland    2.4 Adult Carcass    4.1 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    136.575 Larval Midgut    4.4 Larval Hindgut    6.4 Larval Malpighian Tubules    6.9 Larval Fat Body no informative data Larval Salivary Gland    5.1 Larval Trachea no informative data Larval Carcass no informative data Adult Head    3.6 Adult Eye    7.925 Adult Brain    6.2 Adult Thoracic-Abdominal Ganglion    4.3 Adult Crop    3.7 Adult Midgut    3.1 Adult Hindgut    3.9 Adult Malpighian Tubules    4.5 Adult Fat Body    5 Adult Salivary Gland    4.9 Adult Heart    3.25 Adult VirginFemale Spermatheca    3.9 Adult InseminatedFemale Spermatheca    3.2 Adult Ovary    4.6 Adult Testis    3.6 Adult Male Accessory Gland    2.4 Adult Carcass    4.1 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    136.575 Larval Midgut    4.4 Larval Hindgut    6.4 Larval Malpighian Tubules    6.9 Larval Fat Body no informative data Larval Salivary Gland    5.1 Larval Trachea no informative data Larval Carcass no informative data Adult Head    3.6 Adult Eye    7.925 Adult Brain    6.2 Adult Thoracic-Abdominal Ganglion    4.3 Adult Crop    3.7 Adult Midgut    3.1 Adult Hindgut    3.9 Adult Malpighian Tubules    4.5 Adult Fat Body    5 Adult Salivary Gland    4.9 Adult Heart    3.25 Adult VirginFemale Spermatheca    3.9 Adult InseminatedFemale Spermatheca    3.2 Adult Ovary    4.6 Adult Testis    3.6 Adult Male Accessory Gland    2.4 Adult Carcass    4.1 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body no informative data Larval Salivary Gland     Larval Trachea no informative data Larval Carcass no informative data Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    3.6   NA  Eye    7.925   NA  Brain    6.2   136.575  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    4.3   NA  Crop    3.7   4.4  Midgut    3.1   6.4  Hindgut    3.9   6.9  Malpighian Tubules    4.5   no informative data  Fat Body    5   5.1  Salivary Gland    4.9   NA  Heart    3.25   no informative data  Trachea    NA   NA  VirginFemale Spermatheca    3.9   NA  InseminatedFemale Spermatheca    3.2   NA  Ovary    4.6   NA  Testis    3.6   NA  Male Accessory Gland    2.4   no informative data  Carcass    4.1 modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0002633 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG8361-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | pupal stage, with Scer\GAL4h-1J3 HLHm7Act.Scer\UAS.T:Hsim\VP16 viable, with Scer\GAL4pnr-MD237 HLHm7GD4359 Other Phenotypes Allele visible, with Scer\GAL432B HLHm7Scer\UAS.cdCa visible, with Scer\GAL4455.2 HLHm7Scer\UAS.cdCa visible, with Scer\GAL4ap-md544 HLHm7Scer\UAS.cdCa visible, with Scer\GAL4dpp.blk1 HLHm7Scer\UAS.cdCa visible, with Scer\GAL4h-1J3 HLHm7Scer\UAS.cdCa visible, with Scer\GAL4pnr-MD237 HLHm7KNEQ.Scer\UAS HLHm7Scer\UAS.cdCa visible, with Scer\GAL4ptc-559.1 HLHm7Act.Scer\UAS.T:Hsim\VP16 HLHm7Scer\UAS.cdCa visible, with Scer\GAL4sca-537.4 HLHm7Scer\UAS.cdCa Phenotype manifest in Allele adult mesothoracic segment, with Scer\GAL4pnr-MD237 HLHm7Scer\UAS.cdCa adult thorax, with Scer\GAL4pnr-MD237 HLHm7KNEQ.Scer\UAS HLHm7Scer\UAS.cdCa chaeta, with Scer\GAL4ap-md544 HLHm7Scer\UAS.cdCa dorsal mesothoracic disc & sensory mother cell, with Scer\GAL4455.2 HLHm7Act.Scer\UAS.T:Hsim\VP16 eye, with Scer\GAL4GMR.PF HLHm7Scer\UAS.cdCa interommatidial bristle, with Scer\GAL4GMR.PF HLHm7Scer\UAS.cdCa intersegmental nerve, with Scer\GAL4elav-C155 HLHm7EP3587 leg & macrochaeta, with Scer\GAL430A HLHm7Act.Scer\UAS.T:Hsim\VP16 HLHm7Scer\UAS.cdCa macrochaeta HLHm7BG02029 macrochaeta, with Scer\GAL4h-1J3 HLHm7ΔbasicAct.Scer\UAS.T:Hsim\VP16 macrochaeta, with Scer\GAL4pnr-MD237 HLHm7KNEQ.Scer\UAS HLHm7Scer\UAS.cdCa macrochaeta, with Scer\GAL4sca-537.4 HLHm7Scer\UAS.cdCa macrochaeta | ectopic, with Scer\GAL4h-1J3 HLHm7KNEQAct.Scer\UAS.T:Hsim\VP16 macrochaeta | ectopic, with Scer\GAL4ptc-559.1 HLHm7Act.Scer\UAS.T:Hsim\VP16 mesothoracic tergum, with Scer\GAL4ap-md544 HLHm7Scer\UAS.cdCa mesothoracic tergum & macrochaeta, with Scer\GAL4sca-C253 HLHm7Scer\UAS.cdCa microchaeta, with Scer\GAL4pnr-MD237 HLHm7KNEQ.Scer\UAS HLHm7Scer\UAS.cdCa scutellar bristle, with Scer\GAL4455.2 HLHm7Scer\UAS.cdCa scutellar bristle, with Scer\GAL4dpp.blk1 HLHm7Scer\UAS.cdCa scutellar bristle, with Scer\GAL4pnr-MD237 HLHm7Scer\UAS.cdCa scutellar bristle, with Scer\GAL4ptc-559.1 HLHm7Scer\UAS.cdCa scutellum & macrochaeta, with Scer\GAL4h-1J3 HLHm7Scer\UAS.cdCa scutum, with Scer\GAL4ptc-559.1 HLHm7Act.Scer\UAS.T:Hsim\VP16 scutum & macrochaeta, with Scer\GAL4ap-md544 HLHm7Scer\UAS.cdCa scutum & macrochaeta, with Scer\GAL4h-1J3 HLHm7ΔbasicAct.Scer\UAS.T:Hsim\VP16 HLHm7Act.Scer\UAS.T:Hsim\VP16 HLHm7KNEQAct.Scer\UAS.T:Hsim\VP16 HLHm7Scer\UAS.cdCa scutum & macrochaeta, with Scer\GAL4ptc-559.1 HLHm7Act.Scer\UAS.T:Hsim\VP16 scutum & microchaeta, with Scer\GAL432B HLHm7Scer\UAS.cdCa scutum & microchaeta, with Scer\GAL4ap-md544 HLHm7Scer\UAS.cdCa sensillum campaniformium of anterior crossvein | ectopic, with Scer\GAL4ptc-559.1 HLHm7Act.Scer\UAS.T:Hsim\VP16 sensory mother cell, with Scer\GAL4sca-C253 HLHm7Scer\UAS.cdCa sensory mother cell | ectopic, with Scer\GAL4455.2 HLHm7Act.Scer\UAS.T:Hsim\VP16 wing, with Scer\GAL4ptc-559.1 HLHm7Act.Scer\UAS.T:Hsim\VP16 wing & macrochaeta, with Scer\GAL469B HLHm7Act.Scer\UAS.T:Hsim\VP16 wing & macrochaeta, with Scer\GAL4ptc-559.1 HLHm7Act.Scer\UAS.T:Hsim\VP16 wing & sensory mother cell, with Scer\GAL4455.2 HLHm7Act.Scer\UAS.T:Hsim\VP16 wing margin bristle, with Scer\GAL432B HLHm7Scer\UAS.cdCa wing vein, with Scer\GAL432B HLHm7Scer\UAS.cdCa wing vein | ectopic, with Scer\GAL469B HLHm7Act.Scer\UAS.T:Hsim\VP16 wing vein L2, with Scer\GAL469B HLHm7Scer\UAS.cdCa wing vein L4, with Scer\GAL469B HLHm7Scer\UAS.cdCa
Classical Alleles ( 5 )
For All Classical Alleles Show
Allele of HLHm7 Class Mutagen Stocks Known lesion HLHm7NP1358 1 -- HLHm7NP1360 1 -- HLHm7NP1365 1 -- HLHm7BG02029 P-element activity 0 Yes HLHm7EP3587 P-element activity 0 Yes
Alleles Carried on Transgenic Constructs ( 20 )
For All Alleles Carried on Transgenic Constructs Show
Allele of HLHm7 Class Mutagen Stocks Known lesion HLHm7GD4359 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes HLHm7GLV21068 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes HLHm7JF02489 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes HLHm7KK110898 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes HLHm7Scer\UAS.cdCa in vitro construct - regulatory fusion 1 Yes HLHm7+t10 in vitro construct - genomic fragment 0 Yes HLHm7+t4 in vitro construct - genomic fragment 0 Yes HLHm7+t5.6 in vitro construct - genomic fragment 0 Yes HLHm71-143.Scer\UAS in vitro construct - regulatory fusion 0 Yes HLHm7142-186.Act5C.T:Scer\GAL4 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes HLHm7Act.Scer\UAS.T:Hsim\VP16 in vitro construct - coding region fusion 0 Yes HLHm7Act5C.PG in vitro construct - regulatory fusion 0 Yes HLHm7Act5C.PvD in vitro construct - minigene in vitro construct - regulatory fusion 0 Yes HLHm7Act5C.T:Hsim\VP16 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes HLHm7KNEQ.Act5C in vitro construct - regulatory fusion in vitro construct - site directed mutagenesis in vitro construct - amino acid replacement 0 Yes HLHm7KNEQ.Scer\UAS in vitro construct - regulatory fusion in vitro construct - amino acid replacement 0 Yes HLHm7KNEQAct.Scer\UAS.T:Hsim\VP16 in vitro construct - deletion 0 Yes HLHm7ΔbasicAct.Scer\UAS.T:Hsim\VP16 in vitro construct - deletion 0 Yes HLHm7ΔW.Act5C in vitro construct - regulatory fusion 0 Yes HLHm7ΔW.Scer\UAS in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3R)boss9 (Fischer-Vize et al., 1992) Df(3R)boss16 (Heitzler et al., 1996) Df(3R)E(spl)-rv27 (Tietze et al., 1993, Heitzler et al., 1996, de Celis et al., 1996) Df(3R)Espl4 (Heitzler et al., 1996) Df(3R)Espl9 (Fischer-Vize et al., 1992) Df(3R)Espl10 (Fischer-Vize et al., 1992) Df(3R)Espl22 (Ligoxygakis et al., 1998, Fischer-Vize et al., 1992) Df(3R)Exel6204 (Ryder, 2004.4.26) Df(3R)grob32.2 (Ligoxygakis et al., 1998, Heitzler et al., 1996, de Celis et al., 1996, de Celis et al., 1997) Df(3R)P11 (Nagel et al., 2000) Df(3R)P709 (Fischer-Vize et al., 1992) T(2;3)boss3 (Fischer-Vize et al., 1992)
Not disrupted in	Df(3R)boss15 (Nagel et al., 2000, Fischer-Vize et al., 1992) Df(3R)E(spl)δ-6 (Chanet et al., 2009)
Partially disrupted in	Df(3R)E(spl)δ-6 (Bardin et al., 2010)
Duplicated in	Dp(3;2)E(spl)δ-8 (Chanet et al., 2009) Dp(3;2)E(spl)δ-8Δα46 (Chanet et al., 2009)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data reporter construct P{HLHm70.5-lacZ} No P{HLHm70.5M-lacZ} No UAS construct P{GD4359} NA P{KK110898} NA P{m71-143-UAS} NA P{m7Act} NA P{m7KNEQAct} NA P{m7ΔbasicAct} NA P{TRiP.GLV21068} NA P{TRiP.JF02489} NA P{UAS-HLHm7.C} NA P{UAS-HLHm7.KNEQ} NA P{UAS-HLHm7.ΔW} NA characterization construct P{E(spl)+5.6} NA P{HLHm7,E(spl)+} NA P{HLHm7+} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EP}EP3209 NA P{EP}EP3272 NA P{EP}HLHm7EP3587 NA P{EPg}KrT95DHP30518 NA P{EPgy2}EY02975 NA P{GSV1}EP-469.1EP-469.1 NA miscellaneous insertions P{GT1}HLHm7BG02029 NA P{PTT-GB}CB02243 NA P{SUPor-P}KG02262 NA insertion of enhancer trap binary system P{GawB}DJ913DJ913 No P{GawB}DJ996DJ996 No P{GawB}DJ1026DJ1026 No P{GawB}HLHm7NP1358 No P{GawB}HLHm7NP1360 No P{GawB}HLHm7NP1365 No
Gene Ontology: Function, Process & Cellular Component ( 13 unique terms )
Terms Based on Experimental Evidence ( 9 terms )
Molecular Function
CV term Evidence References
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription inferred from direct assay (van Doren et al., 1994) sequence-specific DNA binding inferred from direct assay (Jennings et al., 1999) transcription factor binding inferred from genetic interaction with da (Giagtzoglou et al., 2003) inferred from genetic interaction with gro (Giagtzoglou et al., 2003) inferred from genetic interaction with sc (Giagtzoglou et al., 2003) inferred from physical interaction with gro (Goldstein et al., 1999)
Biological Process
CV term Evidence References
brain morphogenesis inferred from mutant phenotype (Yamamoto et al., 2008) dendrite morphogenesis inferred from mutant phenotype (Parrish et al., 2006) locomotion involved in locomotory behavior inferred from mutant phenotype (Yamamoto et al., 2008) negative regulation of transcription from RNA polymerase II promoter inferred from direct assay (van Doren et al., 1994, Giagtzoglou et al., 2003) inferred from mutant phenotype (Giagtzoglou et al., 2003) neuron development inferred from mutant phenotype (Parrish et al., 2006) startle response inferred from mutant phenotype (Yamamoto et al., 2008)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 4 terms )
Molecular Function
CV term Evidence References
sequence-specific DNA binding transcription factor activity non-traceable author statement (Swiss-Prot Project Members, 1990.1.1) traceable author statement (Kumar and Moses, 2000)
Biological Process
CV term Evidence References
compound eye development traceable author statement (Kumar and Moses, 2000) regulation of transcription from RNA polymerase II promoter non-traceable author statement (Swiss-Prot Project Members, 1990.1.1)
Cellular Component
CV term Evidence References
nucleus non-traceable author statement (Swiss-Prot Project Members, 1990.1.1)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions HLHm7 allele Gene References HLHm7+t5.6 N (Nagel et al., 1999) HLHm7KNEQ.Scer\UAS sc (Giagtzoglou et al., 2003) HLHm7Scer\UAS.cdCa btl::Egfr (Hasson et al., 2005) N (Ligoxygakis et al., 1999) sc (Giagtzoglou et al., 2003) Su(H) (Klein et al., 2000)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 68060 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0002633 DroID - A comprehensive database of gene and protein interactions. • FBgn0002633 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 43160
Orthologs
Genome-wide drosophilid orthologs	Dana\GF16801 Dere\GG11458 Dgri\GH19458 Dmoj\GI24692 Dper\GL21822 Dpse\GA21018 Dsec\GM10299 Dsim\HLHm7 Dvir\GJ23909 Dwil\GK14459 Dyak\HLHm7
Curated drosophilid orthologs	Dhyd\HLHm7   Dpse\GA21018   Dsim\HLHm7   Dyak\HLHm7
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • 302410 Daphnia pulex (daphnia) ENSDARP00000092351 Danio rerio (zebra fish) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • FBgn0082704 FBgn0216465 FBgn0211002 FBgn0082734 FBgn0165250 FBgn0081007 FBgn0159415 FBgn0147414 FBgn0126923 FBgn0103757 FBgn0093822
Stocks & Reagents
Stocks Listed in FlyBase ( 9 )
Bloomington	26681 y1 w*; P{UAS-HLHm7.C}2 29327 y1 v1; P{TRiP.JF02489}attP2 35703 y1 sc* v1; P{TRiP.GLV21068}attP2
Kyoto	112645 y* w*; P{GawB}HLHm7NP1358 / TM6, P{UAS-lacZ.UW23-1}UW23-1 112646 y* w*; P{GawB}HLHm7NP1360 / TM6, P{UAS-lacZ.UW23-1}UW23-1 112650 y* w*; P{GawB}HLHm7NP1365 / TM6, P{UAS-lacZ.UW23-1}UW23-1
VDRC	v107478 P{KK110898}VIE-260B v16752 w1118; P{GD4359}v16752 v16753 w1118; P{GD4359}v16753
Genomic Clones ( 2 )
	BACR13F13 BACR22N13 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 13 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	IP09063
Other clones	DPiM_IP09063
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones
Other clones	97327 117920 123357 AI124338 CK01.14A.H3 CK01.101D.A12 EK085934 EK237741 EK246263 IP08863 IP09163
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0002633 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 43160
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments
Other Comments
	dsRNA has been made from templates generated with primers directed against this gene. RNAi of HLHm7 results in reduced arborization of ddaD and ddaE neurons, alterations in the number of MD neurons and defects in dendrite morphogenesis. (Parrish et al., 2006) The distinct expression patterns of genes of the E(spl) complex in imaginal tissues depend to a significant degree on the capacity of their transcriptional cis-regulatory apparatus to respond selectively to direct proneural and Su(H)-mediated activation, often in a subset of the territories and cells in which proneural and Su(H) regulation is occurring. (Nellesen et al., 1999) In a sample of 79 genes with multiple introns, 33 showed significant heterogeneity in G+C content among introns of the same gene and significant positive correspondence between the intron and the third codon position G+C content within genes. These results are consistent with selection adding against preferred codons at the start of genes. (Kliman and Eyre-Walker, 1998) E(spl) proteins normally mediate lateral inhibition by directly repressing proneural gene expression. (Jimenez and Ish-Horowicz, 1997) The bHLH domains of the gene products encoded by the E(spl)-C and AS-C differ in their ability to form homo- and/or heterodimers. The interactions established through the bHLH link the products of the two complexes in a single interaction network which may function to ensure that a given cell retains the capacity to choose between epidermoblast and neuroblast fates until the cell becomes definitively determined. (Gigliani et al., 1996) Clones mutant for E(spl)-C bHLH-encoding genes or for gro display bristle hyperplasia. The E(spl)-C genes participate in the N signalling pathway. E(spl)-C mutants are epistatic over a gain of function mutant of N and ac-sc mutants are epistatic over E(spl)-C mutants. (Heitzler et al., 1996) Almost all E(spl)-complex bHLH proteins can homo-hetero-dimerise, but not with the same efficiency. All E(spl)-complex bHLH proteins interact with gro protein via their C-terminal domain. E(spl)-complex bHLH proteins interact with proneural proteins, with members of the E(spl) family exhibiting distinct preferences for different proneural proteins. (Alifragis et al., 1995) The bristle loss phenotype of H mutants can be suppressed by deleting components of the E(spl)-complex. The degree of suppression depends on both the number and identity of E(spl)-complex transcription units removed. (Bang et al., 1995) E(spl) bHLH proteins are turned on in cells which are inhibited from becoming neural by signals from the delaminating neuroblast. (Jennings et al., 1995) Electrophoretic mobility shift assays demonstrate that HLHm7 is directly activated in proneural clusters of the late third-instar wing imaginal disc by protein complexes that include the ac and sc bHLH proteins. (Singson et al., 1994) Arrangement and sequence of E(spl)-complex genes in D.melanogaster and D.hydei revealed that the E(spl)-gene, and the structure of complex are highly conserved, suggesting that each individual gene, as well as the organization of the complex, is of functional importance. (Maier et al., 1993) E(spl) complex gene expression pattern in N and neur mutants suggests the protein is required in dictating cell fates during embryogenesis. (Tata and Hartley, 1993) On basis of cross-hybridization and sequence data the E(spl) HLH genes can be placed into 3 groups. The first includes E(spl) and HLHm5, the second includes HLHm7, HLHm3, HLHmA and HLHmB and the last includes HLHmC. (Delidakis and Artavanis-Tsakonas, 1992) Genes of the E(spl) complex act as a functional unit composed of redundant genes which can partially substitute for each other. Eight E(spl)-region genes are required for the development of neurectodermal cells: HLHmδ, HLHmβ, HLHmγ, HLHm3, HLHm5, HLHm7, E(spl) and gro. The E(spl)-region gene m4 may also play a role in this process. (Schrons et al., 1992) The neurogenic phenotype of various embryonic combinations have been studied and divided into extreme neurogenic embryo, intermediate neurogenic embryos, weak intermediate neurogenic embryos and weak neurogenic embryos. (Delidakis et al., 1991) Genetic analysis demonstrates that Dl, neu, E(spl), HLHm5, HLHm7 and m4 are functionally related. Spatial distribution of mRNA in neurogenic mutant embryos suggests that some of the functional interactions take place at the transcriptional level. (Godt et al., 1991) One of the Enhancer-of-split complex.
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_080505 RefSeq (Proteins) • NP_536753 DDBJ / EMBL / GenBank • AAF56554 AAV59050 AAV59062 AAV59074 AAV59086 AAV59098 AAV59110 AAV59122 AAV59134 AAV59146 AAV59158 AAV59170 AAV59182 AAV59194 AAV59206 AAV59218 AAV59230 AI124338 AQ073039 AY779906 AY779907 AY779908 AY779909 AY779910 AY779911 AY779912 AY779913 AY779914 AY779915 AY779916 AY779917 AY779918 AY779919 AY779920 AY779921 CAA34553 U13069 X16553 Entrez Gene - A searchable database of RefSeq genes. • 43160 UniProtKB/Swiss-Prot • P13097
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Orange subgroup (IPR018352) Orange (IPR003650) Helix-loop-helix DNA-binding (IPR011598)
Linkouts
	BioGRID - A database of protein and genetic interactions • 68060 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0002633 DroID - A comprehensive database of gene and protein interactions. • FBgn0002633 DRSC - Results from RNAi screens. • FBgn0002633 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0002633 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG8361-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0002633 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 43160 InParanoid A subset of ortholog calls from InParanoid. • 302410 Daphnia pulex (daphnia) ENSDARP00000092351 Danio rerio (zebra fish) Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /hjmuller/e-splm7-1.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 43160 modMine - Data generated by the modENCODE project. • FBgn0002633 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • FBgn0082704 FBgn0216465 FBgn0211002 FBgn0082734 FBgn0165250 FBgn0081007 FBgn0159415 FBgn0147414 FBgn0126923 FBgn0103757 FBgn0093822 REDfly - A database of transcriptional regulatory elements. • FBgn0002633
Synonyms & Secondary IDs ( 16 )
Reported As
Symbol Synonym	bHLHb35 (Stevens et al., 2008) CG8361 (Bhutkar et al., 2008, Moehring and Mackay, 2004, Norga et al., 2003, Kraut et al., 2001, Kraut et al., 2001, Molnar et al., 2006, Stevens et al., 2008, Stevens et al., 2008, Krejcí et al., 2009, Keleman et al., 2009.8.5, Perkins et al., 2009, Ni et al., 2011.7.19) E(spl)m7 (Lai et al., 2005, Giagtzoglou et al., 2005, Ahmed et al., 2003, Giagtzoglou et al., 2003, Karandikar et al., 2003, Giagtzoglou et al., 2003, Rebeiz et al., 2002, Lai, 2002, Brody et al., 2002, Rohrbaugh et al., 2002, Giagtzoglou et al., 2001, Ledent and Vervoort, 2001, Trott et al., 2001, Bhaskar et al., 2000, Posakony, 1994.8.5, Ligoxygakis et al., 1999, Liang and Biggin, 1998, Levanon et al., 1998, Lai et al., 1998, Fisher and Caudy, 1998, Schweisguth and Lecourtois, 1998, Leviten et al., 1997, Barbash and Cline, 1995, Roark et al., 1995, Francois et al., 1994, Singson et al., 1994, Wainwright and Ish-Horowicz, 1992, Vaessin et al., 1990, Zarifi et al., 2005, Seidner et al., 2006, Acar et al., 2006, Barry et al., 2011, Kalive et al., 2001, Goodfellow et al., 2007, Sun et al., 2008, Parks et al., 2008, Krejcí et al., 2009, Li et al., 2009, Jones et al., 2009, Ayyar et al., 2007, Moshkin et al., 2009, Di Stefano et al., 2011, van Doren et al., 1994) E(spl) m7 (Campos-Ortega, 1989.9.18) E(spl)-m7 (Goldstein et al., 1999) E(spl)M7 (Jennings et al., 1999) E(spl)-M7 (Jennings et al., 1999) E(SPL)m7 (Abmayr et al., 1992) En(spl)m7 (Mitchell et al., 2008) ESPL (Segev et al., 2001) HLHm7 (Bhutkar et al., 2008, Reeves and Posakony, 2005, Yamamoto et al., 2007, Hoskins et al., 2005, Buszczak et al., 2007, Liu et al., 2005, Blanco et al., 2010, Maeder et al., 2007, Parks and Muskavitch, 2009.2.4, Yamamoto et al., 2008, Lu et al., 2008, Schaaf et al., 2009, Jones et al., 2009, Duncan and Dearden, 2010) HLH-m7 (Tietze et al., 1993, Campos-Ortega, 1993) m7 (Macdonald et al., 2005, Klebes et al., 2005, Kan and Kessler, 2005, Kumar and Moses, 2001, Kumar and Moses, 2001, Kumar and Moses, 2000, Lai et al., 2000, Govind, 1999, Nellesen et al., 1999, Wech et al., 1999, Wurmbach et al., 1999, Wurmbach and Preiss, 1997, Preiss et al., 1997, Dumstrei et al., 1998, Ligoxygakis et al., 1998, Ligoxygakis et al., 1998, Culi and Modolell, 1998, Jimenez and Ish-Horowicz, 1997, Hemavathy et al., 1997, Rusch and Levine, 1996, de Celis et al., 1996, Gigliani et al., 1996, Fisher et al., 1996, Heitzler et al., 1996, Hartenstein et al., 1996, Bang et al., 1995, Wurmbach et al., 1995, Lecourtois and Schweisguth, 1995, Lecourtois and Schweisguth, 1995, Alifragis et al., 1995, Alifragis et al., 1995, Tepass and Hartenstein, 1995, Tietze et al., 1993, Gonzalez-Crespo and Levine, 1993, Schrons et al., 1991, Preiss et al., 1991, Knust et al., 1991, Delidakis and Artavanis-Tsakonas, 1992, Fischer-Vize et al., 1992, Delidakis et al., 1991, Rao et al., 1991, Campos-Ortega and Knust, 1990, Campos-Ortega and Knust, 1990, Klambt et al., 1989, Brody et al., 2006, Eastman et al., 2007, Papatsenko et al., 2006, Nagel et al., 2004, Schlatter and Maier, 2005, Molnar et al., 2006, Assa-Kunik et al., 2007, Krejci and Bray, 2007, Macdonald and Long, 2005, Zinzen et al., 2006, Goodfellow et al., 2007, Maeder et al., 2009, Jones et al., 2009, Chanet et al., 2009, Knust et al., 1987, Moshkin et al., 2009, Li et al., 2009, Bardin et al., 2010, Duncan and Dearden, 2010, Cave et al., 2011) M7 (Razin et al., 1999, Parrish et al., 2006)
Name Synonym	E(spl) region transcript m7 (Parrish et al., 2006, Krejcí et al., 2009) Enhancer of split m7 (Chandra and Vaessin, 2003)
Secondary FlyBase IDs
	FBgn0020009 FBgn0020922
References ( 181 )
Generate a list of	All references relating to this gene ( 181 )
List References by type	Research paper  ( 125 ) Supplementary material  ( 4 ) Review  ( 9 ) Personal communication to FlyBase  ( 7 ) Abstract  ( 26 ) FlyBase analysis  ( 2 ) DNA/RNA sequence record  ( 2 ) Protein sequence record  ( 1 ) Computer file  ( 2 ) Automatic genome annotation  ( 1 ) Curated genome annotation  ( 1 )
Recent research papers ( 8 )
	Barry et al., 2011, Development 138(9): 1759--1769 The Drosophila STUbL protein Degringolade limits HES functions during embryogenesis. [FBrf0213458] Cave et al., 2011, Mol. Cell. Biol. 31(1): 22--29 Differential Regulation of Transcription through Distinct Suppressor of Hairless DNA Binding Site Architectures during Notch Signaling in Proneural Clusters. [FBrf0212524] Di Stefano et al., 2011, Genes Dev. 25(1): 17--28 Functional antagonism between histone H3K4 demethylases in vivo. [FBrf0212709] Lieber et al., 2011, Neuron 69(3): 468--481 DSL-Notch Signaling in the Drosophila Brain in Response to Olfactory Stimulation. [FBrf0212999] Mulligan et al., 2011, Mol. Cell 42(5): 689--699 A SIRT1-LSD1 Corepressor Complex Regulates Notch Target Gene Expression and Development. [FBrf0213865] Bardin et al., 2010, Development 137(5): 705--714 Transcriptional control of stem cell maintenance in the Drosophila intestine. [FBrf0209974] Blanco et al., 2010, BMC Dev. Biol. 10: 94 Gene expression following induction of regeneration in Drosophila wing imaginal discs. Expression profile of regenerating wing discs. [FBrf0211812] Duncan and Dearden, 2010, Genome Res. 20(7): 917--928 Evolution of a genomic regulatory domain: the role of gene co-option and gene duplication in the Enhancer of split complex. [FBrf0211195] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...