FB2020_06, released Dec 22, 2020

Gene: Dmel\Map205

Open Close
General Information
Symbol
Dmel\Map205
Species
D. melanogaster
Name
Microtubule-associated protein 205
Annotation Symbol
CG1483
Feature Type
protein_coding_gene
FlyBase ID
FBgn0002645
Gene Model Status
Current
Stock Availability
17 publicly available
Gene Snapshot
Microtubule-associated protein 205 (Map205) encodes a protein that binds and stabilizes microtubules. It also binds, inhibits and stabilizes the product of polo on microtubules during interphase. [Date last reviewed: 2019-03-14]
Other Summaries
Also Known As

205K MAP

Key Links
Genomic Location
Cytogenetic map
100E3-100E3
Sequence location
3R:32,055,279..32,068,441 [-]
Recombination map

3-103

RefSeq locus
NT_033777 REGION:32055279..32068441
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (5 terms)
Molecular Function (0 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (0 terms)
Biological Process (2 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
involved_in centrosome cycle
inferred from mutant phenotype
(Dobbelaere et al., 2008)
involved_in mitotic cell cycle
inferred from mutant phenotype
(Archambault et al., 2008)
inferred from genetic interaction with FLYBASE:polo; FB:FBgn0003124
(Archambault et al., 2008)
Terms Based on Predictions or Assertions (0 terms)
Cellular Component (3 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
part_of microtubule associated complex
inferred from direct assay
(Irminger-Finger et al., 1990)
located_in midbody
inferred from direct assay
(Dobbelaere et al., 2008)
located_in spindle
inferred from direct assay
(Dobbelaere et al., 2008)
Terms Based on Predictions or Assertions (0 terms)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
    -
    Summaries
    Protein Function (UniProtKB)
    May play an important role in the regulation of microtubule assembly and interaction.
    (UniProt, P23226)
    Gene Model and Products
    Number of Transcripts
    3
    Number of Unique Polypeptides
    3

    Please see the JBrowse view of Dmel\Map205 for information on other features

    To submit a correction to a gene model please use the Contact FlyBase form

    Protein Domains (via Pfam)
    Isoform displayed:
    Pfam protein domains
    InterPro name
    classification
    start
    end
    Protein Domains (via SMART)
    Isoform displayed:
    SMART protein domains
    InterPro name
    classification
    start
    end
    Comments on Gene Model

    Gene model reviewed during 5.47

    Low-frequency RNA-Seq exon junction(s) not annotated.

    Gene model reviewed during 5.53

    Sequence Ontology: Class of Gene
    nuclear_gene
    protein_coding_gene
    Transcript Data
    Annotated Transcripts
    Name
    FlyBase ID
    RefSeq ID
    Length (nt)
    Assoc. CDS (aa)
    Map205-RA
    FBtr0085875
    NM_079876
    4243
    1163
    Map205-RB
    FBtr0085876
    NM_170586
    4733
    1185
    Map205-RC
    FBtr0334299
    NM_001276225
    5818
    1109
    Additional Transcript Data and Comments
    Reported size (kB)
    Comments
    External Data
    Crossreferences
    Polypeptide Data
    Annotated Polypeptides
    Name
    FlyBase ID
    Predicted MW (kDa)
    Length (aa)
    Theoretical pI
    RefSeq ID
    GenBank
    Map205-PA
    FBpp0085234
    124.2
    1163
    4.43
    NP_524615
    AAF57214
    Map205-PB
    FBpp0085235
    126.7
    1185
    4.50
    NP_733465
    AAN14301
    Map205-PC
    FBpp0306414
    118.2
    1109
    4.46
    NP_001263154
    AGB96532
    Polypeptides with Identical Sequences

    None of the polypeptides share 100% sequence identity.

    Additional Polypeptide Data and Comments
    Reported size (kDa)
    Comments
    External Data
    Crossreferences
    Linkouts
    Sequences Consistent with the Gene Model
    Nucleotide / Polypeptide Records
     
    UniProt
    Mapped Features

    Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Map205 using the Feature Mapper tool.

    External Data
    Crossreferences
    Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
    Linkouts
    Expression Data
    Expression Summary Ribbons
    Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
    For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
    Transcript Expression
    Polypeptide Expression
    mass spectroscopy
    Stage
    Tissue/Position (including subcellular localization)
    Reference
    day 7 of adulthood -- day 49 of adulthood
    adult heart
    (Cammarato et al., 2011)
    adult stage
    adult head
    (Aradska et al., 2015)
    Additional Descriptive Data

    Map205 protein isdetected at 25% of wild type levels in the Map20515.6 mutant.

    (Pereira et al., 1992)
    Marker for
     
    Subcellular Localization
    CV Term
    Evidence
    References
    part_of microtubule associated complex
    inferred from direct assay
    (Irminger-Finger et al., 1990)
    located_in midbody
    inferred from direct assay
    (Dobbelaere et al., 2008)
    located_in spindle
    inferred from direct assay
    (Dobbelaere et al., 2008)
    Expression Deduced from Reporters
    High-Throughput Expression Data
    Associated Tools

    GBrowse - Visual display of RNA-Seq signals

    View Dmel\Map205 in GBrowse 2
    RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
    Bulk Downloads
    RNA-Seq RPKM values for all genes
    Reference
    See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
    Developmental Proteome: Life Cycle
    Developmental Proteome: Embryogenesis
    External Data and Images
    Linkouts
    BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
    FLIGHT - Cell culture data for RNAi and other high-throughput technologies
    FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    Flygut - An atlas of the Drosophila adult midgut
    Images
    FlyExpress - Embryonic expression images (BDGP data)
    • Stages(s) 4-6
    • Stages(s) 9-10
    • Stages(s) 11-12
    • Stages(s) 13-16
    FlyBase Wiki
    Image Based Resources
    Alleles, Insertions, and Transgenic Constructs
    Classical and Insertion Alleles ( 20 )
    For All Classical and Insertion Alleles Show
     
    Other relevant insertions
    Transgenic Constructs ( 9 )
    For All Alleles Carried on Transgenic Constructs Show
    Transgenic constructs containing/affecting coding region of Map205
    Transgenic constructs containing regulatory region of Map205
    Deletions and Duplications ( 5 )
    Phenotypes
    For more details about a specific phenotype click on the relevant allele symbol.
    Lethality
    Allele
    viable
    viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
    viable, with Scer\GAL4Mef2.PR
    viable, with Scer\GAL4pnr-MD237
    Sterility
    Allele
    fertile
    Phenotype manifest in
    Allele
    Orthologs
    Downloads
    Download All DIOPT Orthologs
    Download All OrthoDB Orthologs
    Human Orthologs (via DIOPT v8.0)
    Homo sapiens (Human) (0)
    No records found.
    Model Organism Orthologs (via DIOPT v8.0)
    Mus musculus (laboratory mouse) (0)
    No records found.
    Rattus norvegicus (Norway rat) (0)
    No records found.
    Xenopus tropicalis (Western clawed frog) (0)
    No records found.
    Danio rerio (Zebrafish) (0)
    No records found.
    Caenorhabditis elegans (Nematode, roundworm) (0)
    No records found.
    Arabidopsis thaliana (thale-cress) (0)
    No records found.
    Saccharomyces cerevisiae (Brewer's yeast) (0)
    No records found.
    Schizosaccharomyces pombe (Fission yeast) (0)
    No records found.
    Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091902D4 )
    Organism
    Common Name
    Gene
    AAA Syntenic Ortholog
    Multiple Dmel Genes in this Orthologous Group
    Drosophila simulans
    Dsim\GD16184
    Drosophila sechellia
    Dsec\GM16406
    Drosophila erecta
    Dere\GG11842
    Drosophila yakuba
    Dyak\GE23288
    Drosophila ananassae
    Dana\GF17495
    Drosophila pseudoobscura pseudoobscura
    Dpse\GA30152
    Drosophila persimilis
    Dper\GL24098
    Drosophila willistoni
    Dwil\GK10932
    Drosophila virilis
    Dvir\GJ22796
    Drosophila mojavensis
    Dmoj\GI22794
    Drosophila grimshawi
    Dgri\GH17468
    Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091509OU )
    Organism
    Common Name
    Gene
    Multiple Dmel Genes in this Orthologous Group
    Musca domestica
    House fly
    Mdoa\17025052
    Lucilia cuprina
    Australian sheep blowfly
    Lcup\FF38_12760
    Mayetiola destructor
    Hessian fly
    Mdes\Mdes011064
    Anopheles darlingi
    American malaria mosquito
    Adar\ADAC007430
    Anopheles gambiae
    Malaria mosquito
    Agam\AGAP002149
    Culex quinquefasciatus
    Southern house mosquito
    Cqui\CPIJ010234
    Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0AS7 )
    Organism
    Common Name
    Gene
    Multiple Dmel Genes in this Orthologous Group
    Heliconius melpomene
    Postman butterfly
    Hmel\HMEL006933
    Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0APC )
    Organism
    Common Name
    Gene
    Multiple Dmel Genes in this Orthologous Group
    Daphnia pulex
    Water flea
    Dpux\DAPPUDRAFT_265942
    Daphnia pulex
    Water flea
    Dpux\DAPPUDRAFT_322611
    Daphnia pulex
    Water flea
    Dpux\DAPPUDRAFT_310447
    Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( None identified )
    No non-Arthropod Metazoa orthologies identified
    Paralogs
    Downloads
    Download All DIOPT Paralogs
    Paralogs (via DIOPT v8.0)
    Drosophila melanogaster (Fruit fly) (0)
    No records found.
    Human Disease Associations
    FlyBase Human Disease Model Reports
      Disease Model Summary Ribbon
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Allele
      Disease
      Evidence
      References
      Potential Models Based on Orthology ( 0 )
      Human Ortholog
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Allele
      Disease
      Interaction
      References
      Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
      Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
      Homo sapiens (Human)
      Gene name
      Score
      OMIM
      OMIM Phenotype
      DO term
      Complementation?
      Transgene?
      Functional Complementation Data
      Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
      Interactions
      Summary of Physical Interactions
      Summary of Genetic Interactions
      esyN Network Diagram
      esyN Network Key:
      Suppression
      Enhancement
      View in Interactions Browser
      Please look at the allele data for full details of the genetic interactions
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      polo
      enhanceable
      Map205
      (Archambault et al., 2008)
      TBPH
      suppressible
      Map205
      (Vanden Broeck et al., 2013)
      External Data
      Linkouts
      BioGRID - A database of protein and genetic interactions.
      DroID - A comprehensive database of gene and protein interactions.
      InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
      MIST (protein-protein) - An integrated Molecular Interaction Database
      Pathways
      Signaling Pathways (FlyBase)
      Metabolic Pathways
      External Data
      Linkouts
      Genomic Location and Detailed Mapping Data
      Chromosome (arm)
      3R
      Recombination map

      3-103

      Cytogenetic map
      100E3-100E3
      Sequence location
      3R:32,055,279..32,068,441 [-]
      FlyBase Computed Cytological Location
      Cytogenetic map
      Evidence for location
      100E3-100E3
      Limits computationally determined from genome sequence between P{EP}pygoEP1076 and P{PZ}ttk02667&P{lacW}ttkj6C7
      Experimentally Determined Cytological Location
      Cytogenetic map
      Notes
      References
      100E-100F
      (determined by in situ hybridisation)
      (Goldstein et al., 1986)
      Experimentally Determined Recombination Data
      Location

      3-103

      (FlyBase, 2016)

      3-99.1

      (Comeron et al., 2012)
      Left of (cM)
      Right of (cM)
      Notes
      Stocks and Reagents
      Stocks (17)
      Genomic Clones (12)
       

      Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

      cDNA Clones (153)
       

      Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

      cDNA clones, fully sequenced
      BDGP DGC clones
      Other clones
      Drosophila Genomics Resource Center cDNA clones

      For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

      cDNA Clones, End Sequenced (ESTs)
      BDGP DGC clones
      Other clones
      RNAi and Array Information
      Linkouts
      DRSC - Results frm RNAi screens
      GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
      Antibody Information
      Laboratory Generated Antibodies
       
      Commercially Available Antibodies
       
      Other Information
      Relationship to Other Genes
      Source for database identify of

      Source for identity of: Map205 CG1483

      (Zhao, 1999.11)
      Source for database merge of
      Additional comments
      Other Comments

      Map205 is required for efficient recruitment of pericentriolar material.

      (Dobbelaere et al., 2008)

      dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.

      (Kiger et al., 2003)

      Area matching Drosophila MAP gene Acc. No. X54061.

      (Davies, 2001.3.15)

      An antibody against Map205 has been used to clone yeast genes encoding microtubule associated proteins from a yeast cDNA expression library.

      (Irminger-Finger et al., 1996)

      The influence of Map205 on the expression and organisation of tubulin in CHO cells and the effect of the protein on tubulin monomer-polymer pools is compared to these properties of human MAP and tau proteins.

      (Barlow et al., 1994)

      Map205 is not a vital gene, it is not required for development.

      (Pereira et al., 1992)

      Map205 has been cloned and sequenced. Deletion analysis has identified a 232 amino acid region within the Map205 protein (from amino acid 723 to 955) which is necessary for full microtubule-binding activity. The activity of the Map205 protein may be regulated by alternative splicing and phosphorylation.

      (Irminger-Finger et al., 1990)

      Encodes a 205kD microtubule-associated protein, which by immunostaining can be localized to cytoplasmic microtubules and to the mitotic spindle.

      Origin and Etymology
      Discoverer
      Etymology
      Identification
      External Crossreferences and Linkouts ( 45 )
      Sequence Crossreferences
      NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
      GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
      GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
      RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
      UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
      UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
      Other crossreferences
      BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
      Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
      Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
      Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
      Flygut - An atlas of the Drosophila adult midgut
      GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
      KEGG Genes - Molecular building blocks of life in the genomic space.
      modMine - A data warehouse for the modENCODE project
      Linkouts
      BioGRID - A database of protein and genetic interactions.
      DPiM - Drosophila Protein interaction map
      DroID - A comprehensive database of gene and protein interactions.
      DRSC - Results frm RNAi screens
      FLIGHT - Cell culture data for RNAi and other high-throughput technologies
      FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
      FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
      FlyMine - An integrated database for Drosophila genomics
      InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
      MIST (protein-protein) - An integrated Molecular Interaction Database
      Synonyms and Secondary IDs (10)
      Datasets (0)
      Study focus (0)
      Experimental Role
      Project
      Project Type
      Title
      References (98)