• Home
• Tools
  • Tools Overview
  • Query Tools

    
    • QuickSearch
    • QueryBuilder
    • TermLink
    • CytoSearch
    • Interactions Browser
    • ImageBrowse
    • Find A Person
    • Google™ FlyBase
  • Genomic/Map Tools

    
    • BLAST
    • GBrowse
    • CytoSearch
    • Aberrations Maps
    • Chromosome Maps
    • Coordinates Converter
  • Retrieve/Convert

    
    • Batch Download
    • Coordinates Converter
    • ID Converter
  • Data Submission

    
    • Fast-Track Your Paper
    • Submit Personal Comm
    • Add A New Person
    • Update Person Info
  • People

    
    • Find A Person
    • Add A New Person
    • Update Person Info
• Files
  • Files Overview
  • Downloads:
  • Precomputed files
  • Archived data
  • Releases (FTP)
  • Genomes (FTP)
  • AAA data (FTP)
  • Lists:
  • Transposons (Dmel)
  • Transposons (other)
  • Vectors & Constructs
  • Anatomical Terms
  • Map Conversion
• Species
  • Phylogeny
  • Sequenced Species
  • Synteny Table
  • Color Illustrations
  • Abbreviations
• Documents
  • About FlyBase
  • Reference Manual:
  • Sections
  • Contents
  • Nomenclature
  • Release Notes
• Resources
  • All Resources
  • Model Organisms
  • Stock Collections
  • Other links:
  • BDGP
  • DGRC
  • FlyExpress
  • Interactive Fly
  • modENCODE
  • Textpresso for Fly
• News
  • News
  • FlyBase Forum
  • FlyBase Positions
  • Meetings
  • Courses
  • Fly Board
  • White papers
  • Funding
  • bionet.dros
• Help
  • Google™ FlyBase
  • Site Map
  • FAQs
  • FlyBase Guides
  • Video Tutorials
  • Report help
  • New to Flies
  • Pers. comm.
  • Author guidelines
  • Contact FlyBase
• Archives
  • Prior Release
  • Other Archives
  • Coordinate Converter

Gene Dmel\mei-9

General Information
Symbol	Dmel\mei-9	Species	D. melanogaster
Name	meiotic 9	Annotation symbol	CG3697
Feature type	protein_coding_gene	FlyBase ID	FBgn0002707
Gene Model Status	Current	Stock availability	48 publicly available
Also Known As	mei9
Genomic Location
Chromosome (arm)	X	Recombination map	1-6.5
Cytogenetic map	4B6-4B6	Sequence location	X:4,208,263..4,212,834 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene meiotic 9 is referred to in FlyBase by the symbol Dmel\mei-9 (CG3697, FBgn0002707). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: protein binding; protein heterodimerization activity. There is experimental evidence that it is involved in the biological process: meiotic chromosome segregation; female meiosis chromosome segregation; reciprocal meiotic recombination; mismatch repair; mitotic cell cycle G2/M transition DNA damage checkpoint; resolution of meiotic recombination intermediates; nucleotide-excision repair. 25 alleles are reported. The phenotypes of these alleles are annotated with: presumptive oocyte; egg chamber. It has one annotated transcript and one annotated polypeptide. Protein features are: DNA repair nuclease, XPF-type/Helicase; DNA repair protein; ERCC4 domain; Restriction endonuclease, type II-like; RuvA domain 2-like. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderate expression to a trough of very low expression. Peak expression observed within 00-06 hour embryonic stages, during early pupal stages, in adult female stages. Summary of FlyAtlas Anatomical Expression Data: Little or no expression detected in any larval or adult organs/tissues. Comments on Affy2 ProbeSet: ProbeSet 1624320_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of mei-9. Gene sequence location is X:4208263..4212834.
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	mei-9 (R.S. Hawley) mei-9 alleles confer sensitivity to mutagens as a consequence of a defect in excision repair (Boyd et al., 1976; Nguyen and Boyd, 1977). This defect in DNA repair is also manifested by a high frequency of mitotic chromosome breakage and instability (Baker et al., 1978; Gatti, 1979). For example, larval neuroblasts of mei-9/Y males display a high frequency of spontaneous chromosome breaks in both the eu- and heterochromatin (Gatti, 1979). Females homozygous for mei-9 show greatly reduced levels of meiotic exchange. However, the residual exchanges are distributed as in wild-type and chiasma interference is maintained. mei-9 is thus considered to be defective in the exchange process itself, rather than in the establishment of the preconditions for exchange (Carpenter and Sandler, 1974). mei-9a and mei-9b have also been assayed with respect to their effects on gene conversion at the rosy locus (Carpenter, 1982). Although neither allele reduces the frequency of gene conversion events, both produce post-meiotic segregation events (i.e., mosaic progeny) at high frequency. Thus, the recombinational phenotype of mei-9 involves two components, namely a decrease in the frequency of heteroduplex repair and a decrease in the frequency of reciprocal exchange. At the ultrastructural level, both synaptonemal complex morphology and the number and distribution of recombination nodules are normal in mei-9 females (Carpenter, 1979); but see Boyd et al. (1976). As a consequence of the decreased frequency of reciprocal exchange, mei-9 females display greatly elevated frequencies of meiotic nondisjunction and chromosome loss (Baker and Carpenter, 1972). Meiotic chromosome behavior in males is not affected. Nor is there any effect of mei-9 on spontaneous recombination in males (Lutken and Baker, 1979). Neither hypermutable to alkylation nor deficient in excision repair (Smith and Dusenberry, 1988, Mechanisms and Consequences of DNA Damage Processing, Alan R. Liss, Inc., pp. 251-55).
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2011_10
FB2012_01	Sequence features HFA18792 BKN20736
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 4B6-4B6   Limits computationally determined from genome sequence between P{EP}CG2930EP1352 and P{EP}EP425
Experimentally Determined Cytological Location
Cytogenetic map Notes References 4B4-4B6   (determined by in situ hybridisation)   (Yamamoto et al., 1990)
Experimentally Determined Recombination Data
Location	1- (Smith, 1976) 1-6.5 (Gatti, 1979) 2-5.9--6.5 (Yamamoto and Wada, 1990)
Left of (cM)	rb (Lawlor, 1980) mei-41 (Smith, 1976)
Right of (cM)	ec (Lawlor, 1980)
Notes	Maps near 1-54. (Smith, 1976) Based on the MMS sensitivity of mei-9RT4; alternatively 1-5.9, based on the MMS sensitivity of mei-9RT1.   The methyl methanesulfonate (MMS) sensitivity of mei-9RT1 maps to 2-5.9. The MMS sensitivity of mei-9RT4 maps to 2-6.5. (Yamamoto and Wada, 1990)
Gene Model & Products
Please see the GBrowse view of Dmel\mei-9 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) mei-9-RA FBtr0070656  NM_080329   3606   961
Additional Transcript Data & Comments
Reported size (kB)	3.4 (northern blot) (Sekelsky et al., 1995)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein mei-9-PA   FBpp0070624   109.5   961   6.94     NP_525068   AAF45938
Additional Polypeptide Data & Comments
Reported size (kDa)	926 (aa) (Sekelsky et al., 1995)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • ERCC4 domain (IPR006166) DNA repair protein (IPR006167) Restriction endonuclease, type II-like (IPR011335) DNA repair nuclease, XPF-type/Helicase (IPR020819) RuvA domain 2-like (IPR010994)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE014298 AAF45938   AI531877   BF500351   BG639603   BI228927   BI229167   BI356493   BI365545   BI367759   BI368401   BI482541   BI641398   BT001319 AAN71074   CO332760   CZ483837   EC067839   EC079616   U27181 AAC46917
UniProtKB/Swiss-Prot	Q24087
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0002707    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0002707 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderate expression to a trough of very low expression. Peak expression observed within 00-06 hour embryonic stages, during early pupal stages, in adult female stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0002707 expression level Developmental Stage   Expression Level embryo 00-02hr    1220 embryo 02-04hr    585 embryo 04-06hr    571 embryo 06-08hr    275 embryo 08-10hr    252 embryo 10-12hr    181 embryo 12-14hr    186 embryo 14-16hr    167 embryo 16-18hr    105 embryo 18-20hr    66 embryo 20-22hr    44 embryo 22-24hr    65 larva L1    215 larva L2    232 larva L3 12hr old    189 larva L3 puffstage 1-2    195 larva L3 puffstage 3-6    260 larva L3 puffstage 7-9    289 white prepupae new    308 white prepupae 12hr    329 white prepupae 24hr    441 pupae 2d postWPP    298 pupae 3d postWPP    106 pupae 4d postWPP    94 adult male 01day    115 adult male 05day    130 adult male 30day    164 adult female 01day    518 adult female 05day    1016 adult female 30day    936 Expression Level Scale  None   Extremely low   Very low   Low   Moderate  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (1220) embryo 02-04hr  (585) embryo 04-06hr  (571) embryo 06-08hr  (275) embryo 08-10hr  (252) embryo 10-12hr  (181) embryo 12-14hr  (186) embryo 14-16hr  (167) embryo 16-18hr  105 embryo 18-20hr    66 embryo 20-22hr    44 embryo 22-24hr    65 larva L1  (215) larva L2  (232) larva L3 12hr old  (189) larva L3 puffstage 1-2  (195) larva L3 puffstage 3-6  (260) larva L3 puffstage 7-9  (289) white prepupae new  (308) white prepupae 12hr  (329) white prepupae 24hr  (441) pupae 2d postWPP  (298) pupae 3d postWPP  106 pupae 4d postWPP    94 adult male 01day  (115) adult male 05day  (130) adult male 30day  (164) adult female 01day  (518) adult female 05day  (1016) adult female 30day  (936) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    1220 embryo 02-04hr    585 embryo 04-06hr    571 embryo 06-08hr    275 embryo 08-10hr    252 embryo 10-12hr    181 embryo 12-14hr    186 embryo 14-16hr    167 embryo 16-18hr    105 embryo 18-20hr    66 embryo 20-22hr    44 embryo 22-24hr    65 larva L1    215 larva L2    232 larva L3 12hr old    189 larva L3 puffstage 1-2    195 larva L3 puffstage 3-6    260 larva L3 puffstage 7-9    289 white prepupae new    308 white prepupae 12hr    329 white prepupae 24hr    441 pupae 2d postWPP    298 pupae 3d postWPP    106 pupae 4d postWPP    94 adult male 01day    115 adult male 05day    130 adult male 30day    164 adult female 01day    518 adult female 05day    1016 adult female 30day    936 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    1220 embryo 02-04hr    585 embryo 04-06hr    571 embryo 06-08hr    275 embryo 08-10hr    252 embryo 10-12hr    181 embryo 12-14hr    186 embryo 14-16hr    167 embryo 16-18hr    105 embryo 18-20hr    66 embryo 20-22hr    44 embryo 22-24hr    65 larva L1    215 larva L2    232 larva L3 12hr old    189 larva L3 puffstage 1-2    195 larva L3 puffstage 3-6    260 larva L3 puffstage 7-9    289 white prepupae new    308 white prepupae 12hr    329 white prepupae 24hr    441 pupae 2d postWPP    298 pupae 3d postWPP    106 pupae 4d postWPP    94 adult male 01day    115 adult male 05day    130 adult male 30day    164 adult female 01day    518 adult female 05day    1016 adult female 30day    936 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    1220 embryo 02-04hr    585 embryo 04-06hr    571 embryo 06-08hr    275 embryo 08-10hr    252 embryo 10-12hr    181 embryo 12-14hr    186 embryo 14-16hr    167 embryo 16-18hr    105 embryo 18-20hr    66 embryo 20-22hr    44 embryo 22-24hr    65 larva L1    215 larva L2    232 larva L3 12hr old    189 larva L3 puffstage 1-2    195 larva L3 puffstage 3-6    260 larva L3 puffstage 7-9    289 white prepupae new    308 white prepupae 12hr    329 white prepupae 24hr    441 pupae 2d postWPP    298 pupae 3d postWPP    106 pupae 4d postWPP    94 adult male 01day    115 adult male 05day    130 adult male 30day    164 adult female 01day    518 adult female 05day    1016 adult female 30day    936 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0002707 expression level Developmental Stage   Expression Level embryo 00-02hr    1220 embryo 02-04hr    585 embryo 04-06hr    571 embryo 06-08hr    275 embryo 08-10hr    252 embryo 10-12hr    181 embryo 12-14hr    186 embryo 14-16hr    167 embryo 16-18hr    105 embryo 18-20hr    66 embryo 20-22hr    44 embryo 22-24hr    65 larva L1    215 larva L2    232 larva L3 12hr old    189 larva L3 puffstage 1-2    195 larva L3 puffstage 3-6    260 larva L3 puffstage 7-9    289 white prepupae new    308 white prepupae 12hr    329 white prepupae 24hr    441 pupae 2d postWPP    298 pupae 3d postWPP    106 pupae 4d postWPP    94 adult male 01day    115 adult male 05day    130 adult male 30day    164 adult female 01day    518 adult female 05day    1016 adult female 30day    936 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (1220) embryo 02-04hr  (585) embryo 04-06hr  (571) embryo 06-08hr  (275) embryo 08-10hr  (252) embryo 10-12hr  (181) embryo 12-14hr  (186) embryo 14-16hr  (167) embryo 16-18hr  105 embryo 18-20hr    66 embryo 20-22hr    44 embryo 22-24hr    65 larva L1  (215) larva L2  (232) larva L3 12hr old  (189) larva L3 puffstage 1-2  (195) larva L3 puffstage 3-6  (260) larva L3 puffstage 7-9  (289) white prepupae new  (308) white prepupae 12hr  (329) white prepupae 24hr  (441) pupae 2d postWPP  (298) pupae 3d postWPP  106 pupae 4d postWPP    94 adult male 01day  115 adult male 05day  130 adult male 30day  (164) adult female 01day  (518) adult female 05day  (1016) adult female 30day  (936) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    1220 embryo 02-04hr    585 embryo 04-06hr    571 embryo 06-08hr    275 embryo 08-10hr    252 embryo 10-12hr    181 embryo 12-14hr    186 embryo 14-16hr    167 embryo 16-18hr    105 embryo 18-20hr    66 embryo 20-22hr    44 embryo 22-24hr    65 larva L1    215 larva L2    232 larva L3 12hr old    189 larva L3 puffstage 1-2    195 larva L3 puffstage 3-6    260 larva L3 puffstage 7-9    289 white prepupae new    308 white prepupae 12hr    329 white prepupae 24hr    441 pupae 2d postWPP    298 pupae 3d postWPP    106 pupae 4d postWPP    94 adult male 01day    115 adult male 05day    130 adult male 30day    164 adult female 01day    518 adult female 05day    1016 adult female 30day    936 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    1220 embryo 02-04hr    585 embryo 04-06hr    571 embryo 06-08hr    275 embryo 08-10hr    252 embryo 10-12hr    181 embryo 12-14hr    186 embryo 14-16hr    167 embryo 16-18hr    105 embryo 18-20hr    66 embryo 20-22hr    44 embryo 22-24hr    65 larva L1    215 larva L2    232 larva L3 12hr old    189 larva L3 puffstage 1-2    195 larva L3 puffstage 3-6    260 larva L3 puffstage 7-9    289 white prepupae new    308 white prepupae 12hr    329 white prepupae 24hr    441 pupae 2d postWPP    298 pupae 3d postWPP    106 pupae 4d postWPP    94 adult male 01day    115 adult male 05day    130 adult male 30day    164 adult female 01day    518 adult female 05day    1016 adult female 30day    936 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    1220 embryo 02-04hr    585 embryo 04-06hr    571 embryo 06-08hr    275 embryo 08-10hr    252 embryo 10-12hr    181 embryo 12-14hr    186 embryo 14-16hr    167 embryo 16-18hr    105 embryo 18-20hr    66 embryo 20-22hr    44 embryo 22-24hr    65 larva L1    215 larva L2    232 larva L3 12hr old    189 larva L3 puffstage 1-2    195 larva L3 puffstage 3-6    260 larva L3 puffstage 7-9    289 white prepupae new    308 white prepupae 12hr    329 white prepupae 24hr    441 pupae 2d postWPP    298 pupae 3d postWPP    106 pupae 4d postWPP    94 adult male 01day    115 adult male 05day    130 adult male 30day    164 adult female 01day    518 adult female 05day    1016 adult female 30day    936 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0002707    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0002707 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Little or no expression detected in any larval or adult organs/tissues. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0002707 expression level Tissue   Expression Level Larval Central Nervous System    70.05 Larval Midgut no informative data Larval Hindgut    18.6 Larval Malpighian Tubules    23.5 Larval Fat Body no informative data Larval Salivary Gland    28.5 Larval Trachea    47.4 Larval Carcass    24.875 Adult Head no informative data Adult Eye no informative data Adult Brain    35.5 Adult Thoracic-Abdominal Ganglion    25.5 Adult Crop no informative data Adult Midgut no informative data Adult Hindgut no informative data Adult Malpighian Tubules no informative data Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart no informative data Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca no informative data Adult Ovary    64.2 Adult Testis no informative data Adult Male Accessory Gland    38.6 Adult Carcass    9.6 Expression Level Scale  None   Low  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    70.05 Larval Midgut no informative data Larval Hindgut    18.6 Larval Malpighian Tubules    23.5 Larval Fat Body no informative data Larval Salivary Gland    28.5 Larval Trachea    47.4 Larval Carcass    24.875 Adult Head no informative data Adult Eye no informative data Adult Brain    35.5 Adult Thoracic-Abdominal Ganglion    25.5 Adult Crop no informative data Adult Midgut no informative data Adult Hindgut no informative data Adult Malpighian Tubules no informative data Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart no informative data Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca no informative data Adult Ovary    64.2 Adult Testis no informative data Adult Male Accessory Gland    38.6 Adult Carcass    9.6 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    70.05 Larval Midgut no informative data Larval Hindgut    18.6 Larval Malpighian Tubules    23.5 Larval Fat Body no informative data Larval Salivary Gland    28.5 Larval Trachea    47.4 Larval Carcass    24.875 Adult Head no informative data Adult Eye no informative data Adult Brain    35.5 Adult Thoracic-Abdominal Ganglion    25.5 Adult Crop no informative data Adult Midgut no informative data Adult Hindgut no informative data Adult Malpighian Tubules no informative data Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart no informative data Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca no informative data Adult Ovary    64.2 Adult Testis no informative data Adult Male Accessory Gland    38.6 Adult Carcass    9.6 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    70.05 Larval Midgut no informative data Larval Hindgut    18.6 Larval Malpighian Tubules    23.5 Larval Fat Body no informative data Larval Salivary Gland    28.5 Larval Trachea    47.4 Larval Carcass    24.875 Adult Head no informative data Adult Eye no informative data Adult Brain    35.5 Adult Thoracic-Abdominal Ganglion    25.5 Adult Crop no informative data Adult Midgut no informative data Adult Hindgut no informative data Adult Malpighian Tubules no informative data Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart no informative data Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca no informative data Adult Ovary    64.2 Adult Testis no informative data Adult Male Accessory Gland    38.6 Adult Carcass    9.6 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0002707 expression level Tissue   Expression Level Larval Central Nervous System    70.05 Larval Midgut no informative data Larval Hindgut    18.6 Larval Malpighian Tubules    23.5 Larval Fat Body no informative data Larval Salivary Gland    28.5 Larval Trachea    47.4 Larval Carcass    24.875 Adult Head no informative data Adult Eye no informative data Adult Brain    35.5 Adult Thoracic-Abdominal Ganglion    25.5 Adult Crop no informative data Adult Midgut no informative data Adult Hindgut no informative data Adult Malpighian Tubules no informative data Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart no informative data Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca no informative data Adult Ovary    64.2 Adult Testis no informative data Adult Male Accessory Gland    38.6 Adult Carcass    9.6 Expression Level Scale  None   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    70.05 Larval Midgut no informative data Larval Hindgut    18.6 Larval Malpighian Tubules    23.5 Larval Fat Body no informative data Larval Salivary Gland    28.5 Larval Trachea    47.4 Larval Carcass    24.875 Adult Head no informative data Adult Eye no informative data Adult Brain    35.5 Adult Thoracic-Abdominal Ganglion    25.5 Adult Crop no informative data Adult Midgut no informative data Adult Hindgut no informative data Adult Malpighian Tubules no informative data Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart no informative data Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca no informative data Adult Ovary    64.2 Adult Testis no informative data Adult Male Accessory Gland    38.6 Adult Carcass    9.6 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    70.05 Larval Midgut no informative data Larval Hindgut    18.6 Larval Malpighian Tubules    23.5 Larval Fat Body no informative data Larval Salivary Gland    28.5 Larval Trachea    47.4 Larval Carcass    24.875 Adult Head no informative data Adult Eye no informative data Adult Brain    35.5 Adult Thoracic-Abdominal Ganglion    25.5 Adult Crop no informative data Adult Midgut no informative data Adult Hindgut no informative data Adult Malpighian Tubules no informative data Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart no informative data Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca no informative data Adult Ovary    64.2 Adult Testis no informative data Adult Male Accessory Gland    38.6 Adult Carcass    9.6 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    70.05 Larval Midgut no informative data Larval Hindgut    18.6 Larval Malpighian Tubules    23.5 Larval Fat Body no informative data Larval Salivary Gland    28.5 Larval Trachea    47.4 Larval Carcass    24.875 Adult Head no informative data Adult Eye no informative data Adult Brain    35.5 Adult Thoracic-Abdominal Ganglion    25.5 Adult Crop no informative data Adult Midgut no informative data Adult Hindgut no informative data Adult Malpighian Tubules no informative data Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart no informative data Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca no informative data Adult Ovary    64.2 Adult Testis no informative data Adult Male Accessory Gland    38.6 Adult Carcass    9.6 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut no informative data Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body no informative data Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head no informative data Adult Eye no informative data Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop no informative data Adult Midgut no informative data Adult Hindgut no informative data Adult Malpighian Tubules no informative data Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart no informative data Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca no informative data Adult Ovary     Adult Testis no informative data Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    no informative data   NA  Eye    no informative data   NA  Brain    35.5   70.05  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    25.5   NA  Crop    no informative data   no informative data  Midgut    no informative data   18.6  Hindgut    no informative data   23.5  Malpighian Tubules    no informative data   no informative data  Fat Body    no informative data   28.5  Salivary Gland    no informative data   NA  Heart    no informative data   47.4  Trachea    NA   NA  VirginFemale Spermatheca    no informative data   NA  InseminatedFemale Spermatheca    no informative data   NA  Ovary    64.2   NA  Testis    no informative data   NA  Male Accessory Gland    38.6   24.875  Carcass    9.6 modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_06
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0002707 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG3697-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | partially mei-9A1 mei-9a viable mei-9EY14578 mei-9f01366 viable, with Scer\GAL4pnr-MD237 mei-9GD4109 Sterility Allele female fertile mei-9D1 mei-9D2 mei-9D3 mei-9D4 female sterile mei-9a mei-9RT1 mei-9RT2 mei-9RT4 female sterile | partially | recessive mei-9A1 fertile mei-9EY14578 mei-9f01366 Other Phenotypes Allele chemical sensitive mei-9A1 mei-9A2 mei-9A3 mei-9D1 mei-9D2 mei-9D3 mei-9D4 mei-9L1 mei-9RT1 mei-9RT2 mei-9RT4 mei-9unspecified chemical sensitive (with mei-911) mei-9A2 chemical sensitive (with mei-9A2) mei-911 mei-9a mei-9b mei-9D2 mei-9D4 mei-9L1 mei-9RT1 mei-9RT3 chemical sensitive (with mei-9a) mei-9A2 chemical sensitive (with mei-9b) mei-9A2 chemical sensitive (with mei-9D2) mei-9A2 chemical sensitive (with mei-9D4) mei-9A2 chemical sensitive (with mei-9L1) mei-9A2 chemical sensitive (with mei-9RT1) mei-9A2 chemical sensitive (with mei-9RT3) mei-9A2 chemical sensitive | recessive mei-9A1 DNA repair defective mei-9a mei-9D2 mei-9unspecified increased cell death | conditional mei-9A1 meiotic cell cycle defective mei-9A2 mei-9a mei-9b mitotic cell cycle defective | recessive mei-9A1 mei-9a mei-9b radiation sensitive mei-9A1 mei-9A2 mei-9a mei-9D2 mei-9unspecified radiation sensitive (with mei-911) mei-9A2 radiation sensitive (with mei-9A2) mei-911 mei-9a mei-9b mei-9D2 mei-9D4 mei-9L1 mei-9RT1 mei-9RT3 radiation sensitive (with mei-9a) mei-9A2 radiation sensitive (with mei-9b) mei-9A2 radiation sensitive (with mei-9D2) mei-9A2 radiation sensitive (with mei-9D4) mei-9A2 radiation sensitive (with mei-9L1) mei-9A2 radiation sensitive (with mei-9RT1) mei-9A2 radiation sensitive (with mei-9RT3) mei-9A2 radiation sensitive | recessive mei-9A1 short lived mei-9a wild-type mei-9+tSa Phenotype manifest in Allele egg chamber mei-9a eye | conditional mei-9A1 mitosis & nuclear chromosome mei-9A1 mei-9a mei-9b presumptive oocyte mei-912 mei-9A2 mei-9a
Classical Alleles ( 22 )
For All Classical Alleles Show
Allele of mei-9 Class Mutagen Stocks Known lesion mei-9a ethyl methanesulfonate 34 Yes mei-9A1 ethyl methanesulfonate 2 Yes mei-9f01366 piggyBac transposase 2 -- mei-9L1 ethyl methanesulfonate 2 Yes mei-95-HA-2773 1 -- mei-9A2 ethyl methanesulfonate 1 Yes mei-9b hypomorphic allele - genetic evidence ethyl methanesulfonate 1 Yes mei-9D2 ethyl methanesulfonate 1 Yes mei-9D4 ethyl methanesulfonate 1 Yes mei-9EY14578 P-element activity 1 -- mei-9RT4 PM hybrid dysgenesis 1 -- mei-911 ethyl methanesulfonate 0 Yes mei-912 chemical ethyl methanesulfonate 0 Yes mei-95-HA-2551 0 -- mei-9A3 0 Yes mei-9D1 ethyl methanesulfonate 0 -- mei-9D3 ethyl methanesulfonate 0 Yes mei-9j3 ethyl methanesulfonate 0 -- mei-9RT1 PM hybrid dysgenesis P-element activity 0 Yes mei-9RT2 PM hybrid dysgenesis 0 Yes mei-9RT3 PM hybrid dysgenesis 0 Yes mei-9unspecified 0 --
Alleles Carried on Transgenic Constructs ( 3 )
For All Alleles Carried on Transgenic Constructs Show
Allele of mei-9 Class Mutagen Stocks Known lesion mei-9GD4109 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes mei-9+tSa in vitro construct - genomic fragment 0 Yes mei-9Ubi.T:Zzzz\FLAG in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(1)bi-D1 (Banga et al., 1986) Df(1)bi-DL1 (Banga et al., 1986) Df(1)bi-DL2 (Banga et al., 1986) Df(1)bi-DL4 (Banga et al., 1986) Df(1)bi-DL6 (Banga et al., 1986) Df(1)HC163B (Lawlor, 1980) Df(1)HC244 (Lawlor, 1980, Oliver et al., 1988) Df(1)HF366 (Oliver et al., 1988, Smith et al., 1993) Df(1)rb1 (Oliver et al., 1988) Df(1)rb30 (Banga et al., 1986, Oliver et al., 1988) Df(1)rb32 (Banga et al., 1986) Df(1)rb33 (Oliver et al., 1988) Df(1)rb47 (Oliver et al., 1988) Df(1)RC40 (Banga et al., 1986, Oliver et al., 1988)
Not disrupted in	Df(1)biD1Tp (Banga et al., 1986) Df(1)bi-D2 (Banga et al., 1986, Oliver et al., 1988) Df(1)bi-D3 (Banga et al., 1986) Df(1)C149 (Oliver et al., 1988) Df(1)GA56 (Banga et al., 1986, Oliver et al., 1988) Df(1)GA102 (Oliver et al., 1988) Df(1)JC70 (Oliver et al., 1988) Df(1)N73 (Oliver et al., 1988) Df(1)ovo6 (Oliver et al., 1988) Df(1)ovo7 (Oliver et al., 1988) Df(1)rb13 (Oliver et al., 1988) Df(1)rb41 (Banga et al., 1986)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD4109} NA characterization construct P{mei-9+} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions P{}mei-9RT1 NA P{}mei-9RT4 NA P{RS5}mei-95-HA-2551 NA P{RS5}mei-95-HA-2773 NA PBac{WH}mei-9f01366 NA insertion of mobile activating element P{EPgy2}mei-9EY14578 NA
Gene Ontology: Function, Process & Cellular Component ( 15 unique terms )
Terms Based on Experimental Evidence ( 10 terms )
Molecular Function
CV term Evidence References
protein binding inferred from physical interaction with Ercc1 AND inferred from physical interaction with mus312 (Yildiz et al., 2002) protein heterodimerization activity inferred from physical interaction with Ercc1 (Sekelsky et al., 2000)
Biological Process
CV term Evidence References
female meiosis chromosome segregation inferred from mutant phenotype (Baker and Carpenter, 1972) meiotic chromosome segregation inferred from mutant phenotype (Yildiz et al., 2002) mismatch repair inferred from mutant phenotype (Bhui-Kaur et al., 1998) mitotic cell cycle G2/M transition DNA damage checkpoint inferred from genetic interaction with mus81 (Kondo and Perrimon, 2011) nucleotide-excision repair inferred from mutant phenotype (Boyd et al., 1976) reciprocal meiotic recombination inferred from mutant phenotype (Yildiz et al., 2002, Baker and Carpenter, 1972) resolution of meiotic recombination intermediates inferred from mutant phenotype (Radford et al., 2007)
Cellular Component
CV term Evidence References
protein complex inferred from direct assay (Radford et al., 2005)
Terms Based on Predictions or Assertions ( 8 terms )
Molecular Function
CV term Evidence References
DNA binding inferred from electronic annotation with InterPro:IPR006166, InterPro:IPR020819 (FlyBase Curators et al., 2004-) endodeoxyribonuclease activity inferred from sequence or structural similarity with mouse Ercc4 AND inferred from sequence or structural similarity with UniProtKB:Q92889 (FlyBase, 1992-)
Biological Process
CV term Evidence References
DNA recombination non-traceable author statement (Swiss-Prot Project Members, 1998.12.15) traceable author statement (Sekelsky et al., 2000) double-strand break repair traceable author statement (Sekelsky et al., 2000) mismatch repair non-traceable author statement (Swiss-Prot Project Members, 1998.12.15) nucleotide-excision repair inferred from sequence or structural similarity (Sekelsky et al., 1995) non-traceable author statement (Swiss-Prot Project Members, 1998.12.15) traceable author statement (Sekelsky et al., 2000) resolution of meiotic recombination intermediates traceable author statement (Yildiz et al., 2002)
Cellular Component
CV term Evidence References
nucleus non-traceable author statement (Swiss-Prot Project Members, 1998.12.15)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions mei-9 allele Gene References mei-9a rad201 (Sarantseva and Khromykh, 2001)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 57890 DroID - A comprehensive database of gene and protein interactions. • FBgn0002707 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 31373
Orthologs
Genome-wide drosophilid orthologs	Dana\GF21474 Dere\GG18547 Dgri\GH24669 Dmoj\GI16132 Dper\GL14183 Dpse\GA17620 Dsec\GM12692 Dsim\mei-9 Dvir\GJ16410 Dwil\GK17500 Dyak\GE16862
Curated drosophilid orthologs	Dpse\GA17620   Dsim\mei-9 (Begun, 2000.4.5)
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AAEL002098-PA Aedes aegypti (mosquito) AGAP002805-PA Anopheles gambiae (mosquito) XP_001947198.1 Acyrthosiphon pisum (pea aphid) At5g41150.1 Arabidopsis thaliana (wall cress) 30944 Batrachochytrium dendrobatidis (chytrid fungus) 100820 Branchiostoma floridae (lancelet) XP_001900850.1 Brugia malayi (roundworm nematode) ENSBTAP00000043645 Bos taurus (bovine) CAL0004266 Candida albicans (a form of yeast) CBP00797 Caenorhabditis briggsae (nematode) CE24855 Caenorhabditis elegans (worm) ENSCAFP00000027778 Canis familiaris (dog) CAGL0J09526g Candida glabrata (haploid yeast) ENSCINP00000024250 Ciona intestinalis (sea squirt) CMH034C Cyanidioschyzon merolae (red alga) cn12184 Cryptococcus neoformans (yeast-like fungus) CPIJ004313 Culex pipens (mosquito) ENSCPOP00000004055 Cavia porcellus (guinea pig) 169954 Chlamidomonas reinhardtii (green alga) ENSCSAVP00000001829 Ciona savignyi (sedentary tunicate) 178005 Capitella spl (polychaete worm) DDB0232364 Dictyostelium discoideum (amoeba) DEHA2F08932g Debaryomyces Hansenii (yeast) 331812 Daphnia pulex (daphnia) ENSDARP00000096592 Danio rerio (zebra fish) ENSECAP00000008548 Equus caballus (horse) XP_657509 Entamoeba histolytica (anaerobic protozoan) ENSGACP00000005852 Gymnopilus aculeatus (mushroom) ENSGALP00000004707 Gallus gallus (chicken) 98781 Hypsipyla robusta (cedar shoot borer) ENSP00000310520 Homo sapiens (human) ISCW003047-PA Ixodes scapularis (deer tick) KLLA0D12210g Kluyveromyces lactis (yeast) 104289 Lottia gigantea (owl limpet) LmjF08.0140 Leishmania major (virus) 13638 Monocosta brevicollis (choanoflagellate) ENSMODP00000005962 Monodelphis domestica (gray opossum) ENSMMUP00000020550 Macaca mulatta (rhesus monkey) ENSMUSP00000023206 Mus musculus (house mouse) 168557 Nematostella vectensis (starlet anemone) XP_001600324.1 Nasonia vitripennis (wasp) ENSORLP00000003684 Oryzias latipes (japanese killifish) LOC_Os03g01100.1 Oryza sativa (asian rice) 8629 Ostreococcus tauri (green alga) MAL13P1.346 Plasmodium falciparum (protozoal parasite) PHUM004123-PA Pediculus humanus (human body louse) 201653 Physcomitrella patens (moss) ENSPPYP00000008050 Pongo pygmaeus (orangutan) 74744 Phytophthora ramorum (sudden oak death pathogen) 129382 Phytophthora sojae (root rot pathogen) 589928 Populus trichocarpa (black cottonwood) ENSPTRP00000053757 Pan troglodytes (chimpanzee) PVX_115400 Plasmodium vivax (protozoal parasite) ENSRNOP00000004563 Rattus norvegicus (norway rat) 142265 Sorghum bicolor (sorghum) YPL022W Saccharomyces cerevisiae (budding yeast) Smp_153770 Schistosoma mansoni (trematode parasite) SPCC970.01 Schizosaccharomyces pombe (fission yeast) 50503 Trichoplax adhaerens (metazoa) TA04325 Theileria annulata (apicomplexan parasite) XP_001812786.1 Tribolium castaneum (red flour beetle) Tc00.1047053509779.10 Trypanosoma cruzi (parasitic trypanosome) Tc00.1047053511653.69 Trypanosoma cruzi (parasitic trypanosome) ENSTNIP00000015899 Tetraodon nigroviridis (green spotted pufferfish) TP03_0395 Theileria parva (parasitic protozoan) ENSTRUP00000043440 Takifugu rubripes (pufferfish Takifugu) 95725.m00313 Trichomonas vaginalis (parasitic flagellated protozoan) ENSXETP00000024054 Xenopus tropicalis (frog) YALI0B22242g Yarrowia lipolytica (nonconventional yeast) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV15404 LH15570 ISCW003047 FBgn0234354 FBgn0219499 FBgn0203594 FBgn0187927 FBgn0041662 FBgn0167628 JGI_V11_331812 FBgn0077631 FBgn0151788 FBgn0138881 FBgn0132125 FBgn0110760 FBgn0098475 CPIJ004313 BGIBMGA005180 BGIBMGA004957 ACYPI000872 GB12119 GB11091 AGAP002805 AAEL002098 PB22046 PHUM489110 GLEAN_13011
Stocks & Reagents
Stocks Listed in FlyBase ( 48 )
Bloomington	6792 w1 mei-9A1 18430 w1118 PBac{WH}mei-9f01366 21102 y1 w67c23 P{EPgy2}mei-9EY14578 4280 w1 mei-9A2/C(1)DX, y1 f1
Harvard	- PBac{WH}mei-9f01366
Kyoto	101155 mei-9L1 125667 w1118 P{RS5}mei-95-HA-2773 108392 mei-9a sw20-14/FM7c, ctS 101007 C(1)DX, y1 f1 / w1 mei-9A1 101005 C(1)DX, y1 f1 / sn3 mei-9D2 101006 C(1)DX, y1 f1 / sn3 mei-9D4
VDRC	v12670 w1118; P{GD4109}v12670/TM3
	More stocks available...
Genomic Clones ( 1 )
	BACR33H02 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 13 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	AT15210
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	RE26587 RE26891 RE42660 RE50901 RE53596 RE54366 RE65243 SD24695
Other clones	277173 CK01.112D.E4 EK304462 SD03162
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0002707 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 31373
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: mei-9 CG3697 (Zhong, 1999.11)
Source for database merge of
Additional comments
Other Comments
	The underlying meiotic function for mei-9 appears to be in crossover formation rather than mismatch repair, as in the absence of mei-9 activity, postmeiotic segregation associated with noncrossovers occurs at the expense of crossover products. (Radford et al., 2007) Extracts from Drosophila embryos and adults generated from mei-9 mutants have specific defects in DNA mismatch repair. (Bhui-Kaur et al., 1998) Gene is involved in pre-replication DNA repair of UV and AA lesions. (de Buendia, 1998) Sex-linked recessive lethal (SLRL) test is used to demonstrate inactivation of the nucleotide excision repair (NER) system has a major impact on mutational response of germ cells to mutagens. (Vogel and Nivard, 1997) A positional mapping strategy combined with a differential expression assay is used to clone mei-9, mei-9 is homologous to yeast repair gene RAD1, and the mei-9 transcript is absent in mei-9A3 mutants. (Araj and Smith, 1996) The DNA-damaging activity of 6 phenazine and aminophenazine derivatives is assayed using the wing spot test in larvae, chemicals exhibit significant mutagenicity. (Watanabe et al., 1996) DNA repair test is used to evaluate the genotoxic effect of griseofulvin in somatic larval cells. (Inoue et al., 1995) mei-9 encodes a homologue of the yeast excision repair protein Rad1, which is not required for meiotic recombination in S.cerevisiae. (Sekelsky et al., 1995) The mei-9/mei-41 DNA repair test has been used to assay the DNA-damaging potency of aflatoxin B1 and aflatoxin M1. (Shibahara et al., 1995) Genotoxic potency of aromatic amines and polycyclic aromatic hydrocarbons can be assayed using the DNA repair assay in mei-9a mei-41D5 transheterozygotes. (Fujikawa et al., 1993) The genotoxicity of a series of N-nitrosamines has been assayed using meiotic recombination deficient mei-9a; mei-41D5 larvae. (Negishi et al., 1991) The effects of repair deficiency are studied by comparing the frequency of somatic mutation and mitotic recombination in repair proficient female progeny with that in excision repair defective male progeny. Nine chemical mutagens with various modes of action are tested in this way. (Graf et al., 1990) An unusually high level of P-M hybrid dysgenesis is characteristic of hybrid offspring originating from Harwich P strain crosses. The phenotype is greatly exacerbated when males are deficient either in excision repair (mei-9 mutation) or in post-replication repair (mei-41 mutation). These findings demonstrate that both DNA repair pathways are essential for the repair of lesions induced by P-element transposition. (Margulies, 1990) mei-9 does not play the primary role in determining the frequency of P element excision or the variable nature of RpII2159 revertants. (Voelker et al., 1984) mei-9 alleles confer sensitivity to mutagens as a consequence of a defect in excision repair (Boyd et al., 1976; Nguyen and Boyd, 1977). This defect in DNA repair is also manifested by a high frequency of mitotic chromosome breakage and instability (Baker et al., 1978; Gatti, 1979). For example, larval neuroblasts of mei-9/Y males display a high frequency of spontaneous chromosome breaks in both the eu- and heterochromatin (Gatti, 1979). Females homozygous for mei-9 show greatly reduced levels of meiotic exchange. However, the residual exchanges are distributed as in wild-type and chiasma interference is maintained. mei-9 is thus considered to be defective in the exchange process itself, rather than in the establishment of the preconditions for exchange (Carpenter and Sandler, 1974). mei-9a and mei-9b have also been assayed with respect to their effects on gene conversion at the rosy locus (Carpenter, 1982). Although neither allele reduces the frequency of gene conversion events, both produce post-meiotic segregation events (i.e., mosaic progeny) at high frequency. Thus, the recombinational phenotype of mei-9 involves two components, namely a decrease in the frequency of heteroduplex repair and a decrease in the frequency of reciprocal exchange. At the ultrastructural level, both synaptonemal complex morphology and the number and distribution of recombination nodules are normal in mei-9 females (Carpenter, 1979); but see Boyd et al. (1976). As a consequence of the decreased frequency of reciprocal exchange, mei-9 females display greatly elevated frequencies of meiotic nondisjunction and chromosome loss (Baker and Carpenter, 1972). Meiotic chromosome behavior in males is not affected. Nor is there any effect of mei-9 on spontaneous recombination in males (Lutken and Baker, 1979). Neither hypermutable to alkylation nor deficient in excision repair (Smith and Dusenberry, 1988).
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_080329 RefSeq (Proteins) • NP_525068 DDBJ / EMBL / GenBank • AAC46917 AAF45938 AAN71074 BF500351 BT001319 CZ483837 U27181 Entrez Gene - A searchable database of RefSeq genes. • 31373 UniProtKB/Swiss-Prot • Q24087
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • ERCC4 domain (IPR006166) DNA repair protein (IPR006167) Restriction endonuclease, type II-like (IPR011335) DNA repair nuclease, XPF-type/Helicase (IPR020819) RuvA domain 2-like (IPR010994)
Linkouts
	BioGRID - A database of protein and genetic interactions • 57890 DroID - A comprehensive database of gene and protein interactions. • FBgn0002707 DRSC - Results from RNAi screens. • FBgn0002707 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0002707 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG3697-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0002707 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 31373 InParanoid A subset of ortholog calls from InParanoid. • AAEL002098-PA Aedes aegypti (mosquito) AGAP002805-PA Anopheles gambiae (mosquito) XP_001947198.1 Acyrthosiphon pisum (pea aphid) At5g41150.1 Arabidopsis thaliana (wall cress) 30944 Batrachochytrium dendrobatidis (chytrid fungus) 100820 Branchiostoma floridae (lancelet) XP_001900850.1 Brugia malayi (roundworm nematode) ENSBTAP00000043645 Bos taurus (bovine) CAL0004266 Candida albicans (a form of yeast) CBP00797 Caenorhabditis briggsae (nematode) CE24855 Caenorhabditis elegans (worm) ENSCAFP00000027778 Canis familiaris (dog) CAGL0J09526g Candida glabrata (haploid yeast) ENSCINP00000024250 Ciona intestinalis (sea squirt) CMH034C Cyanidioschyzon merolae (red alga) cn12184 Cryptococcus neoformans (yeast-like fungus) CPIJ004313 Culex pipens (mosquito) ENSCPOP00000004055 Cavia porcellus (guinea pig) 169954 Chlamidomonas reinhardtii (green alga) ENSCSAVP00000001829 Ciona savignyi (sedentary tunicate) 178005 Capitella spl (polychaete worm) DDB0232364 Dictyostelium discoideum (amoeba) DEHA2F08932g Debaryomyces Hansenii (yeast) 331812 Daphnia pulex (daphnia) ENSDARP00000096592 Danio rerio (zebra fish) ENSECAP00000008548 Equus caballus (horse) XP_657509 Entamoeba histolytica (anaerobic protozoan) ENSGACP00000005852 Gymnopilus aculeatus (mushroom) ENSGALP00000004707 Gallus gallus (chicken) 98781 Hypsipyla robusta (cedar shoot borer) ENSP00000310520 Homo sapiens (human) ISCW003047-PA Ixodes scapularis (deer tick) KLLA0D12210g Kluyveromyces lactis (yeast) 104289 Lottia gigantea (owl limpet) LmjF08.0140 Leishmania major (virus) 13638 Monocosta brevicollis (choanoflagellate) ENSMODP00000005962 Monodelphis domestica (gray opossum) ENSMMUP00000020550 Macaca mulatta (rhesus monkey) ENSMUSP00000023206 Mus musculus (house mouse) 168557 Nematostella vectensis (starlet anemone) XP_001600324.1 Nasonia vitripennis (wasp) ENSORLP00000003684 Oryzias latipes (japanese killifish) LOC_Os03g01100.1 Oryza sativa (asian rice) 8629 Ostreococcus tauri (green alga) MAL13P1.346 Plasmodium falciparum (protozoal parasite) PHUM004123-PA Pediculus humanus (human body louse) 201653 Physcomitrella patens (moss) ENSPPYP00000008050 Pongo pygmaeus (orangutan) 74744 Phytophthora ramorum (sudden oak death pathogen) 129382 Phytophthora sojae (root rot pathogen) 589928 Populus trichocarpa (black cottonwood) ENSPTRP00000053757 Pan troglodytes (chimpanzee) PVX_115400 Plasmodium vivax (protozoal parasite) ENSRNOP00000004563 Rattus norvegicus (norway rat) 142265 Sorghum bicolor (sorghum) YPL022W Saccharomyces cerevisiae (budding yeast) Smp_153770 Schistosoma mansoni (trematode parasite) SPCC970.01 Schizosaccharomyces pombe (fission yeast) 50503 Trichoplax adhaerens (metazoa) TA04325 Theileria annulata (apicomplexan parasite) XP_001812786.1 Tribolium castaneum (red flour beetle) Tc00.1047053509779.10 Trypanosoma cruzi (parasitic trypanosome) Tc00.1047053511653.69 Trypanosoma cruzi (parasitic trypanosome) ENSTNIP00000015899 Tetraodon nigroviridis (green spotted pufferfish) TP03_0395 Theileria parva (parasitic protozoan) ENSTRUP00000043440 Takifugu rubripes (pufferfish Takifugu) 95725.m00313 Trichomonas vaginalis (parasitic flagellated protozoan) ENSXETP00000024054 Xenopus tropicalis (frog) YALI0B22242g Yarrowia lipolytica (nonconventional yeast) InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 31373 modMine - Data generated by the modENCODE project. • FBgn0002707 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV15404 LH15570 ISCW003047 FBgn0234354 FBgn0219499 FBgn0203594 FBgn0187927 FBgn0041662 FBgn0167628 JGI_V11_331812 FBgn0077631 FBgn0151788 FBgn0138881 FBgn0132125 FBgn0110760 FBgn0098475 CPIJ004313 BGIBMGA005180 BGIBMGA004957 ACYPI000872 GB12119 GB11091 AGAP002805 AAEL002098 PB22046 PHUM489110 GLEAN_13011
Synonyms & Secondary IDs ( 12 )
Reported As
Symbol Synonym	CG3697 (Gandhi et al., 2007) DhR1 (de Buendia, 1998) DmXPF (Dusenbery and Smith, 1996) mei9 (de Buendia et al., 2003, Cohen et al., 2003, Pastink and Lohman, 1999, Flores and Engels, 1999, Sarantseva and Khromykh, 1999, de Buendia, 1998, Vogel and Nivard, 1997, Varentsova et al., 1997, Sarantseva and Khromykh, 1997, de Buendia, 1995, Smith et al., 1993, Oliver et al., 1988, Koana et al., 2001) MEI-9 (Yildiz et al., 2000) mei-9 (Mukai et al., 2006, Heyer et al., 2003, Radford et al., 2005, Sekelsky et al., 2000, Radford et al., 2007, Blanton et al., 2005, Baker and Carpenter, 1972, Trowbridge et al., 2007, Wei and Rong, 2007, Johnson-Schlitz et al., 2007, Joyce and McKim, 2009, Anderson et al., 2009, Joyce et al., 2009, Andersen et al., 2009, Meyer et al., 2010, Koana and Tsujimura, 2010, Kondo and Perrimon, 2011, Reis et al., 2011, Mishra et al., 2011) mus110   mus(1)110 (Smith, 1976) Rad1p (Johnson-Schlitz et al., 2007) xpf/mei-9 (Jassim et al., 2003)
Name Synonym	meiotic 9   meiotic-9 (Mukai et al., 2006)
Secondary FlyBase IDs
References ( 167 )
Generate a list of	All references relating to this gene ( 167 )
List References by type	Research paper  ( 104 ) Supplementary material  ( 5 ) Review  ( 11 ) Personal communication to FlyBase  ( 4 ) Abstract  ( 29 ) FlyBase analysis  ( 2 ) Curated genome annotation  ( 2 ) Protein sequence record  ( 1 ) Computer file  ( 3 ) Letter  ( 1 ) DNA/RNA sequence record  ( 1 ) Automatic genome annotation  ( 1 ) Book  ( 1 ) Stock list  ( 1 )
Recent research papers ( 6 )
	Kondo and Perrimon, 2011, Sci. Signal. 4(154): rs1 A Genome-Wide RNAi Screen Identifies Core Components of the G2-M DNA Damage Checkpoint. [FBrf0212700] Mishra et al., 2011, Mutat. Res. 722(1): 44--51 Tracing the tracks of genotoxicity by trivalent and hexavalent chromium in Drosophila melanogaster. [FBrf0213784] Reis et al., 2011, PLoS ONE 6(3): e17512 Drosophila Genes That Affect Meiosis Duration Are among the Meiosis Related Genes That Are More Often Found Duplicated. [FBrf0213266] Joyce and McKim, 2010, PLoS Genet. 6(8): Chromosome Axis Defects Induce a Checkpoint-Mediated Delay and Interchromosomal Effect on Crossing Over during Drosophila Meiosis. [FBrf0211491] Koana and Tsujimura, 2010, Radiat. Res. 174(1): 46--51 A U-Shaped Dose-Response Relationship between X Radiation and Sex-Linked Recessive Lethal Mutation in Male Germ Cells of Drosophila. [FBrf0211476] Meyer et al., 2010, BMC Genet. 11(1): 104 A single mutation results in diploid gamete formation and parthenogenesis in a Drosophila yemanuclein-alpha meiosis I defective mutant. [FBrf0212440] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...