General Information
Symbol
Dmel\mei-9
Species
D. melanogaster
Name
meiotic 9
Annotation Symbol
CG3697
Feature Type
FlyBase ID
FBgn0002707
Gene Model Status
Stock Availability
Gene Snapshot
Meiotic 9 (Mei-9) is the catalytic subunit of the Mei-9-Ercc1 structure-selective endonuclease that functions in nucleotide excision repair. Mei-9 and Ercc1 interact with mus312 to generate meiotic crossovers. [Date last reviewed: 2016-06-23]
Also Known As
mei9, mus110
Genomic Location
Cytogenetic map
Sequence location
X:4,314,230..4,318,801 [-]
Recombination map
1-7
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
GO Summary Ribbons
Families, Domains and Molecular Function
Gene Group Membership (FlyBase)
Protein Family (UniProt, Sequence Similarities)
Belongs to the XPF family. (Q24087)
Molecular Function (see GO section for details)
Summaries
UniProt Contributed Function Data
Implicated in recombination events during meiosis, mostly in meiotic exchange. May directly resolve Holliday junctions within recombination intermediates leading to DNA exchange. Also required for the repair of mismatches within meiotic heteroduplex DNA and for nucleotide excision repair.
(UniProt, Q24087)
Phenotypic Description from the Red Book (Lindsley and Zimm 1992)
mei-9 (R.S. Hawley)
mei-9 alleles confer sensitivity to mutagens as a consequence of a defect in excision repair (Boyd et al., 1976; Nguyen and Boyd, 1977). This defect in DNA repair is also manifested by a high frequency of mitotic chromosome breakage and instability (Baker et al., 1978; Gatti, 1979). For example, larval neuroblasts of mei-9/Y males display a high frequency of spontaneous chromosome breaks in both the eu- and heterochromatin (Gatti, 1979). Females homozygous for mei-9 show greatly reduced levels of meiotic exchange. However, the residual exchanges are distributed as in wild-type and chiasma interference is maintained. mei-9 is thus considered to be defective in the exchange process itself, rather than in the establishment of the preconditions for exchange (Carpenter and Sandler, 1974). mei-9a and mei-9b have also been assayed with respect to their effects on gene conversion at the rosy locus (Carpenter, 1982). Although neither allele reduces the frequency of gene conversion events, both produce post-meiotic segregation events (i.e., mosaic progeny) at high frequency. Thus, the recombinational phenotype of mei-9 involves two components, namely a decrease in the frequency of heteroduplex repair and a decrease in the frequency of reciprocal exchange. At the ultrastructural level, both synaptonemal complex morphology and the number and distribution of recombination nodules are normal in mei-9 females (Carpenter, 1979); but see Boyd et al. (1976). As a consequence of the decreased frequency of reciprocal exchange, mei-9 females display greatly elevated frequencies of meiotic nondisjunction and chromosome loss (Baker and Carpenter, 1972). Meiotic chromosome behavior in males is not affected. Nor is there any effect of mei-9 on spontaneous recombination in males (Lutken and Baker, 1979). Neither hypermutable to alkylation nor deficient in excision repair (Smith and Dusenberry, 1988, Mechanisms and Consequences of DNA Damage Processing, Alan R. Liss, Inc., pp. 251-55).
Gene Model and Products
Number of Transcripts
2
Number of Unique Polypeptides
1

Please see the GBrowse view of Dmel\mei-9 or the JBrowse view of Dmel\mei-9 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.52
Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0070656
3606
961
FBtr0343574
3491
961
Additional Transcript Data and Comments
Reported size (kB)
3.4 (northern blot)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0070624
109.5
961
6.94
FBpp0310174
109.5
961
6.94
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

961 aa isoforms: mei-9-PA, mei-9-PB
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Subunit Structure (UniProtKB)
Heterodimer (By similarity). Interacts with hdm.
(UniProt, Q24087)
Crossreferences
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\mei-9 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Gene Ontology (21 terms)
Molecular Function (5 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
inferred from physical interaction with UniProtKB:Q9VS48
(assigned by UniProt )
inferred from physical interaction with FLYBASE:mus312; FB:FBgn0002909
inferred from physical interaction with FLYBASE:Ercc1; FB:FBgn0028434
inferred from physical interaction with FLYBASE:hdm; FB:FBgn0029977
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000015911
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000015911
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000015911
(assigned by GO_Central )
Biological Process (14 terms)
Terms Based on Experimental Evidence (10 terms)
CV Term
Evidence
References
Terms Based on Predictions or Assertions (7 terms)
CV Term
Evidence
References
traceable author statement
inferred from biological aspect of ancestor with PANTHER:PTN000015911
(assigned by GO_Central )
traceable author statement
inferred from sequence or structural similarity
inferred from biological aspect of ancestor with PANTHER:PTN000015911
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000015911
(assigned by GO_Central )
traceable author statement
inferred from biological aspect of ancestor with PANTHER:PTN000015911
(assigned by GO_Central )
Cellular Component (2 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000015911
(assigned by GO_Central )
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\mei-9 in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
Alleles, Insertions, Transgenic Constructs and Phenotypes
Classical and Insertion Alleles ( 24 )
Transgenic Constructs ( 7 )
Deletions and Duplications ( 26 )
Summary of Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Sterility
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
mitosis & nuclear chromosome
Orthologs
Human Orthologs (via DIOPT v7.1)
Homo sapiens (Human) (2)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
14 of 15
Yes
Yes
1 of 15
No
No
Model Organism Orthologs (via DIOPT v7.1)
Mus musculus (laboratory mouse) (2)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
14 of 15
Yes
Yes
1 of 15
No
No
Rattus norvegicus (Norway rat) (4)
3 of 13
Yes
Yes
2 of 13
No
Yes
1 of 13
No
Yes
1 of 13
No
Yes
Xenopus tropicalis (Western clawed frog) (1)
8 of 12
Yes
Yes
Danio rerio (Zebrafish) (2)
14 of 15
Yes
Yes
Caenorhabditis elegans (Nematode, roundworm) (2)
14 of 15
Yes
Yes
1 of 15
No
Yes
Arabidopsis thaliana (thale-cress) (1)
9 of 9
Yes
Yes
Saccharomyces cerevisiae (Brewer's yeast) (1)
15 of 15
Yes
Yes
Schizosaccharomyces pombe (Fission yeast) (1)
12 of 12
Yes
Yes
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG091902ND )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila melanogaster
fruit fly
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila simulans
Drosophila sechellia
Drosophila erecta
Drosophila yakuba
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila persimilis
Drosophila willistoni
Drosophila virilis
Drosophila mojavensis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG0915029P )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Glossina morsitans
Tsetse fly
Lucilia cuprina
Australian sheep blowfly
Mayetiola destructor
Hessian fly
Aedes aegypti
Yellow fever mosquito
Anopheles darlingi
American malaria mosquito
Anopheles gambiae
Malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W03GO )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bombyx mori
Silkmoth
Danaus plexippus
Monarch butterfly
Heliconius melpomene
Postman butterfly
Apis florea
Little honeybee
Apis mellifera
Western honey bee
Bombus impatiens
Common eastern bumble bee
Bombus terrestris
Buff-tailed bumblebee
Linepithema humile
Argentine ant
Megachile rotundata
Alfalfa leafcutting bee
Nasonia vitripennis
Parasitic wasp
Dendroctonus ponderosae
Mountain pine beetle
Tribolium castaneum
Red flour beetle
Pediculus humanus
Human body louse
Rhodnius prolixus
Kissing bug
Cimex lectularius
Bed bug
Acyrthosiphon pisum
Pea aphid
Zootermopsis nevadensis
Nevada dampwood termite
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X03DI )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Ixodes scapularis
Black-legged tick
Stegodyphus mimosarum
African social velvet spider
Tetranychus urticae
Two-spotted spider mite
Daphnia pulex
Water flea
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0554 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Ciona intestinalis
Vase tunicate
Gallus gallus
Domestic chicken
Human Disease Model Data
FlyBase Human Disease Model Reports
    Alleles Reported to Model Human Disease (Disease Ontology)
    Download
    Models ( 0 )
    Allele
    Disease
    Evidence
    References
    Interactions ( 1 )
    Allele
    Disease
    Interaction
    References
    Comments ( 0 )
     
    Human Orthologs (via DIOPT v7.1)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    esyN Network Diagram
    Show neighbor-neighbor interactions:
    Select Layout:
    Legend:
    Protein
    RNA
    Selected Interactor(s)
    Interactions Browser

    Please look at the Interaction Group reports for full details of the physical interactions
    protein-protein
    Interacting group
    Assay
    References
    Summary of Genetic Interactions
    esyN Network Diagram
    esyN Network Key:
    Suppression
    Enhancement

    Please look at the allele data for full details of the genetic interactions
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    External Data
    Subunit Structure (UniProtKB)
    Heterodimer (By similarity). Interacts with hdm.
    (UniProt, Q24087 )
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DroID - A comprehensive database of gene and protein interactions.
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    Pathways
    Gene Group - Pathway Membership (FlyBase)
    External Data
    Linkouts
    KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
    Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    X
    Recombination map
    1-7
    Cytogenetic map
    Sequence location
    X:4,314,230..4,318,801 [-]
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    4B6-4B6
    Limits computationally determined from genome sequence between P{EP}CG2930EP1352 and P{EP}EP425
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    4B4-4B6
    (determined by in situ hybridisation)
    Experimentally Determined Recombination Data
    Right of (cM)
    Notes
    The methyl methanesulfonate (MMS) sensitivity of mei-9RT1 maps to 2-5.9. The MMS sensitivity of mei-9RT4 maps to 2-6.5.
    Maps near 1-54.
    Based on the MMS sensitivity of mei-9RT4; alternatively 1-5.9, based on the MMS sensitivity of mei-9RT1.
    Stocks and Reagents
    Stocks (31)
    Genomic Clones (20)
    cDNA Clones (14)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequences
    BDGP DGC clones
    Other clones
    Drosophila Genomics Resource Center cDNA clones

    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

    cDNA Clones, End Sequenced (ESTs)
    RNAi and Array Information
    Linkouts
    DRSC - Results frm RNAi screens
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    Antibody Information
    Laboratory Generated Antibodies
     
    Commercially Available Antibodies
     
    Other Information
    Relationship to Other Genes
    Source for database identify of
    Source for identity of: mei-9 CG3697
    Source for database merge of
    Additional comments
    Other Comments
    The underlying meiotic function for mei-9 appears to be in crossover formation rather than mismatch repair, as in the absence of mei-9 activity, postmeiotic segregation associated with noncrossovers occurs at the expense of crossover products.
    Extracts from Drosophila embryos and adults generated from mei-9 mutants have specific defects in DNA mismatch repair.
    Gene is involved in pre-replication DNA repair of UV and AA lesions.
    Sex-linked recessive lethal (SLRL) test is used to demonstrate inactivation of the nucleotide excision repair (NER) system has a major impact on mutational response of germ cells to mutagens.
    A positional mapping strategy combined with a differential expression assay is used to clone mei-9, mei-9 is homologous to yeast repair gene RAD1, and the mei-9 transcript is absent in mei-9A3 mutants.
    The DNA-damaging activity of 6 phenazine and aminophenazine derivatives is assayed using the wing spot test in larvae, chemicals exhibit significant mutagenicity.
    DNA repair test is used to evaluate the genotoxic effect of griseofulvin in somatic larval cells.
    mei-9 encodes a homologue of the yeast excision repair protein Rad1, which is not required for meiotic recombination in S.cerevisiae.
    The mei-9/mei-41 DNA repair test has been used to assay the DNA-damaging potency of aflatoxin B1 and aflatoxin M1.
    Genotoxic potency of aromatic amines and polycyclic aromatic hydrocarbons can be assayed using the DNA repair assay in mei-9a mei-41D5 transheterozygotes.
    The genotoxicity of a series of N-nitrosamines has been assayed using meiotic recombination deficient mei-9a; mei-41D5 larvae.
    The effects of repair deficiency are studied by comparing the frequency of somatic mutation and mitotic recombination in repair proficient female progeny with that in excision repair defective male progeny. Nine chemical mutagens with various modes of action are tested in this way.
    An unusually high level of P-M hybrid dysgenesis is characteristic of hybrid offspring originating from Harwich P strain crosses. The phenotype is greatly exacerbated when males are deficient either in excision repair (mei-9 mutation) or in post-replication repair (mei-41 mutation). These findings demonstrate that both DNA repair pathways are essential for the repair of lesions induced by P-element transposition.
    mei-9 does not play the primary role in determining the frequency of P element excision or the variable nature of RpII2159 revertants.
    mei-9 alleles confer sensitivity to mutagens as a consequence of a defect in excision repair (Boyd et al., 1976; Nguyen and Boyd, 1977). This defect in DNA repair is also manifested by a high frequency of mitotic chromosome breakage and instability (Baker et al., 1978; Gatti, 1979). For example, larval neuroblasts of mei-9/Y males display a high frequency of spontaneous chromosome breaks in both the eu- and heterochromatin (Gatti, 1979). Females homozygous for mei-9 show greatly reduced levels of meiotic exchange. However, the residual exchanges are distributed as in wild-type and chiasma interference is maintained. mei-9 is thus considered to be defective in the exchange process itself, rather than in the establishment of the preconditions for exchange (Carpenter and Sandler, 1974). mei-9a and mei-9b have also been assayed with respect to their effects on gene conversion at the rosy locus (Carpenter, 1982). Although neither allele reduces the frequency of gene conversion events, both produce post-meiotic segregation events (i.e., mosaic progeny) at high frequency. Thus, the recombinational phenotype of mei-9 involves two components, namely a decrease in the frequency of heteroduplex repair and a decrease in the frequency of reciprocal exchange. At the ultrastructural level, both synaptonemal complex morphology and the number and distribution of recombination nodules are normal in mei-9 females (Carpenter, 1979); but see Boyd et al. (1976). As a consequence of the decreased frequency of reciprocal exchange, mei-9 females display greatly elevated frequencies of meiotic nondisjunction and chromosome loss (Baker and Carpenter, 1972). Meiotic chromosome behavior in males is not affected. Nor is there any effect of mei-9 on spontaneous recombination in males (Lutken and Baker, 1979). Neither hypermutable to alkylation nor deficient in excision repair (Smith and Dusenberry, 1988).
    Origin and Etymology
    Discoverer
    Etymology
    Identification
    External Crossreferences and Linkouts ( 39 )
    Crossreferences
    NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
    GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
    GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
    RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
    UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
    UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
    Other crossreferences
    KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
    Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    Drosophila Genomics Resource Center - Drosophila Genomics Resource Center cDNA clones
    DroID - A comprehensive database of gene and protein interactions.
    DRSC - Results frm RNAi screens
    Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
    FLIGHT - Cell culture data for RNAi and other high-throughput technologies
    FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    Flygut - An atlas of the Drosophila adult midgut
    FlyMine - An integrated database for Drosophila genomics
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    KEGG Genes - Molecular building blocks of life in the genomic space.
    modMine - A data warehouse for the modENCODE project
    Synonyms and Secondary IDs (13)
    Reported As
    Name Synonyms
    Secondary FlyBase IDs
      Datasets (0)
      Study focus (0)
      Experimental Role
      Project
      Project Type
      Title
      References (202)