MeiS332, mei-S322, Shugoshin
Gene model reviewed during 5.51
1.8, 1.75, 1.6, 1.55 (northern blot)
401 (aa); 44 (kD predicted)
Homodimer. Interacts with Incenp.
Phosphorylation by polo-like kinase (PLK) on Thr-331 antagonizes cohesive function. Phosphorylation on Thr-331 at the metaphase anaphase transition leads to its dissociation from centromeres. In contrast, phosphorylation by aurB/ial on either Ser-124, Ser-125 or Ser-126 is required for association with centromeres.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\mei-S332 using the Feature Mapper tool.
GBrowse - Visual display of RNA-Seq signalsView Dmel\mei-S332 in GBrowse 2
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
mei-S332 protein assembles into a multimeric protein complex that localises to the centromeric regions during prometaphase.
mei-S332 is required for the maintenance of sister-chromatid cohesion until anaphase rather than for the establishment of sister-chromatid cohesion in S phase.
mei-S332 is necessary for chromosome segregation and can interact specifically with the centromere.
mei-S332 is required to maintain sister-chromatid cohesion during meiosis in both males an females. Mutations result in precocious separation of sisters after the metaphase I/anaphase I transition when cohesion is restricted to the kinetochore region.
A fusion of mei-S332 to Avic\GFP is fully functional and localises specifically to the centromere region of meiotic chromosomes. When sister chromatids separate at anaphase II mei-S332-Avic\GFP disappears from the chromosomes suggesting that the destruction or release of this protein is required for sister-chromatid separation.
The earliest manifestation of the requirement for mei-S332 can be observed cytologically at mid-late anaphase I. Mutations in mei-S332 do not effect recombination so the locus is not required for homologous pairing. The mei-S332 gene product is not absolutely required during mitosis.
Mutations at ord cause precocious separation of sister chromatids, at earlier stages than do mutations at mei-S332.