FlyBase Gene Report: Dmel\Men

General Information

Symbol

Dmel\Men

Species

D. melanogaster

Name

Malic enzyme

Annotation Symbol

CG10120

Feature Type

protein_coding_gene

FlyBase ID

FBgn0002719

Gene Model Status

Current

Stock Availability

36 publicly available

Enzyme Name (EC)

Malate dehydrogenase (oxaloacetate-decarboxylating) (1.1.1.38)

Malate dehydrogenase (decarboxylating) (1.1.1.39)

Malate dehydrogenase (oxaloacetate-decarboxylating) (NADP(+)) (1.1.1.40)

Gene Summary

Malic enzyme (Men) encodes a dehydrogenase that catalyzes the oxidation of malate to pyruvate, with the concomitant reduction of NADP[+] to NADPH. It functions, along with the products of Idh, Zw, and Pgd, in a small network of NADP reducing enzymes. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Red Book
Interactive Fly

Also Known As

ME, Mdh-NADP, NADP-malic enzyme

Key Links

Genomic Location

Cytogenetic map

87C6-87C7

Sequence location

3R:12,713,103..12,722,442 [-]

Recombination map

3-52

RefSeq locus

NT_033777 REGION:12713103..12722442

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (13 terms)

Molecular Function (4 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables malate dehydrogenase (decarboxylating) (NADP+) activity

inferred from mutant phenotype

(Farkas et al., 2002)

inferred from direct assay

(Baath and Merritt, 2020, Kim et al., 2015, Lee et al., 1979)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

enables malate dehydrogenase (decarboxylating) (NAD+) activity

inferred from electronic annotation with InterPro:IPR001891, InterPro:IPR012301, InterPro:IPR012302, InterPro:IPR015884

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

enables malate dehydrogenase (decarboxylating) (NADP+) activity

inferred from biological aspect of ancestor with PANTHER:PTN000601052

(assigned by GO_Central )

(Gaudet et al., 2011)

enables malic enzyme activity

inferred from biological aspect of ancestor with PANTHER:PTN000601050

(assigned by GO_Central )

(Gaudet et al., 2011)

enables NAD binding

inferred from electronic annotation with InterPro:IPR012302

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

Biological Process (7 terms)

Terms Based on Experimental Evidence (6 terms)

CV Term

Evidence

References

involved_in determination of adult lifespan

inferred from mutant phenotype

(Paik et al., 2012)

involved_in malate metabolic process

inferred from mutant phenotype

(Farkas et al., 2002)

involved_in NADH metabolic process

inferred from direct assay

(Lee et al., 1979)

involved_in NADPH regeneration

inferred from direct assay

(Baath and Merritt, 2020)

involved_in regulation of cell death

inferred from mutant phenotype

(Yang et al., 2010)

involved_in sleep

inferred from mutant phenotype

(Harbison and Sehgal, 2008)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in malate metabolic process

inferred from biological aspect of ancestor with PANTHER:PTN000601050

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in pyruvate metabolic process

inferred from biological aspect of ancestor with PANTHER:PTN000601050

(assigned by GO_Central )

(Gaudet et al., 2011)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

located_in cytosol

inferred from high throughput direct assay

(Lye et al., 2014)

inferred from direct assay

(Farkas et al., 2002)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in mitochondrion

inferred from biological aspect of ancestor with PANTHER:PTN001157886

(assigned by GO_Central )

(Gaudet et al., 2011)

Gene Group (FlyBase)

MALIC ENZYMES

Protein Family (UniProt)

Protein Signatures (InterPro)

Catalytic Activity (EC)

Experimental Evidence

(1) (S)-malate + NADP(+) = pyruvate + CO(2) + NADPH (1.1.1.40)

(2) Oxaloacetate = pyruvate + CO(2) (1.1.1.40)

Predictions / Assertions

(1) (S)-malate + NAD(+) = pyruvate + CO(2) + NADH (1.1.1.38)

(2) Oxaloacetate = pyruvate + CO(2) (1.1.1.38)

(S)-malate + NAD(+) = pyruvate + CO(2) + NADH (1.1.1.39)

(1) (S)-malate + NADP(+) = pyruvate + CO(2) + NADPH (1.1.1.40)

(2) Oxaloacetate = pyruvate + CO(2) (1.1.1.40)

Summaries

Gene Snapshot

Gene Group (FlyBase)

MALIC ENZYMES -: Malic enzymes comprise a family of proteins with multiple isoforms located in different compartments of eukaryotic cells. They are key enzymes regulating malic acid metabolism and catalyze the reversible reaction of malate oxidative decarboxylation of malic acid, producing pyruvic acid and CO[[2]]. They may be NAD[+] or NADP[+]-dependent. (Adapted from PMID:31322479.)

Phenotypic Description (Red Book; Lindsley and Zimm 1992)

Men: Malic enzyme

Structural gene for malic enzyme = (S)-Malate: NADP+ oxidoreductase [MEN (EC 1.1.1.40)], a tetramer with subunit molecular weight of 58,000 (Lee, Langley, and Burkhart, 1978, Anal. Biochem. 86: 697-296) or 67,250 (Geer, Krochko, Oliver, Walker, and Williamson, 1980, Comp. Biochem. Physiol. 65B: 25-34). Biochemical characterization by Geer, et al., (1980). Enzyme known to provide NADP for lipogenesis; levels in larvae increased by dietary carbohydrate and decreased by dietary lipid. Levels normally high in early third-instar larvae, reducing to half in late third instar, rising again in ageing adults. Highest specific activity found in larval fat body and, among cellular fractions, in the cytosol (Geer, Krochko, and Williamson, 1979, Biochem. Genet. 17: 867-79). Histochemical staining of larval imaginal discs reveals enzyme activity localized to nervous elements, including ommatidial precursors, the morphogenetic furrow, and the optic nerve in the eye antennal disc; and the chordotonal organ and the nerve traversing the leg discs; wing, haltere, labial, and genital discs unstained; however genital disc derivatives, the ejaculatory duct and paragonia in males and oviducts of females are stained in adults (Finkbohner, Cunningham, and Kuhn, 1985, Wilhelm Roux's Arch. Dev. Biol. 194: 217-23). Staining of morphogenetic groove absent in D. simulans and in simulans-melanogaster hybrids except when such hybrids contain two copies of 3R from D. melanogaster and none from D. simulans (Kuhn and Sprey, 1987, Genetics 115: 277-81). All null alleles tested survive and are fertile in combination with Df(3R)ry3l, which lacks Men+ (Voelker et al.). The NADPH/NADP ratio in such null genotypes is one-seventh that of wild type (Geer et al., 1979). Presence of an extra dose of the region from 8D10-12 to 9A1-2 results in an approximately 25% increase in MEN activity (Williamson and Bentley, 1983, Genetics 103: 649-58).

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Men for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.39

Gene model reviewed during 5.44

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.48

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Men-RA

FBtr0082661

NM_169479

2767

759

Men-RB

FBtr0082660

NM_080141

2906

763

Men-RC

FBtr0330025

NM_001275580

2364

578

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

Men-PA

FBpp0082130

84.4

759

7.23

NP_731739

AAF54860

Men-PB

FBpp0082129

84.6

763

6.60

NP_524880

AAF54859

Men-PC

FBpp0303058

64.0

578

6.43

NP_001262509

AGB95891

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Men using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Men_1
Men_2

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

primordial germ cell

(Fisher et al., 2012)

embryonic stage 11 -- 12

central brain primordium

(Fisher et al., 2012)

procrystal cell

(Fisher et al., 2012)

embryonic ventral midline neuroblast

(Fisher et al., 2012)

ventral nerve cord primordium

(Fisher et al., 2012)

yolk nucleus

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

day 5 of adulthood & male -- day 7 of adulthood & male

spermatozoon

(Wasbrough et al., 2010)

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in cytosol

inferred from high throughput direct assay

(Lye et al., 2014)

inferred from direct assay

(Farkas et al., 2002)

Expression Deduced from Reporters

Reporter: PBac{806.LOX-SVS-2}Men^CPTI004074

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult brain | faint

Comment: expression throughout adult brain neuropil, higher in outer regions

(Knowles-Barley, 2011.8.24)

adult brain cell body rind

(Knowles-Barley, 2011.8.24)

Reporter: PBac{CPTI-un}Men^CPTI002759

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult brain

Comment: expression throughout adult brain

(Knowles-Barley, 2011.8.24)

adult brain cell body rind

(Knowles-Barley, 2011.8.24)

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Men in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Men

View exonic expression by tissue for Dmel\Men

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0002719

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

47173/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0002719

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0002719

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0002719

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG10120

Flygut - An atlas of the Drosophila adult midgut

FBgn0002719

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\Men

Stages(s) 1-3
Stages(s) 4-6
Stages(s) 9-10
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 86 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 8 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Men

Transgenic constructs containing regulatory region of Men

Aberrations (Deficiencies and Duplications) ( 10 )

Inferred from experimentation ( 10 )

Gene disrupted in

Df(3R)kar3l

(Leonhardt et al., 1993)

Df(3R)kar-lG27

(Leonhardt et al., 1993)

Df(3R)kar-Sz31

(Leonhardt et al., 1993)

Df(3R)ry615

(Leonhardt et al., 1993)

Gene not disrupted in

Df(3R)kar-Sz13

(Leonhardt et al., 1993)

Df(3R)kar-Sz21

(Leonhardt et al., 1993)

Df(3R)kar-Sz29

(Leonhardt et al., 1993)

Df(3R)ry27

(Leonhardt et al., 1993)

Df(3R)ry75

(Leonhardt et al., 1993)

Gene partially disrupted in

Df(3R)PolQ^Δ

(Beagan et al., 2017)

Inferred from location ( 0 )

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

partially lethal

viable

Sterility

Allele

semi-sterile

Other Phenotypes

Allele

abnormal heat stress response | RU486 conditional, with Scer\GAL4^Switch1.106

Men^UAS.cKa

abnormal oxidative stress response | RU486 conditional, with Scer\GAL4^Switch1.106

Men^UAS.cKa

abnormal sleep

Men^BG01628

electrophoretic variant | fast

Men⁶

electrophoretic variant | intermediate

Men⁴

electrophoretic variant | slow

Men²

long lived, with Scer\GAL4^da.Switch.PT

Men^EP1250

long lived | RU486 conditional, with Scer\GAL4^Switch1.106

Men^UAS.cKa

visible, with Scer\GAL4^GMR.PF

Men^EP1250

Phenotype manifest in

Allele

eye, with Scer\GAL4^GMR.PF

Men^EP1250

germline cell | male, with Scer\GAL4^ptc-559.1

Men^EP500

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (3)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\ME1

15 of 15

Yes

Hsap\ME3

12 of 15

Hsap\ME2

9 of 15

Yes

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (3)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Me1

15 of 15

Yes

Mmus\Me3

12 of 15

Mmus\Me2

10 of 15

Yes

Rattus norvegicus (Norway rat) (3)

Rnor\Me1

13 of 13

Yes

Rnor\Me3

11 of 13

Yes

Rnor\Me2

9 of 13

Yes

Xenopus tropicalis (Western clawed frog) (3)

Xtro\me1

9 of 12

Yes

Xtro\me3

8 of 12

Yes

Xtro\me2

7 of 12

Yes

Danio rerio (Zebrafish) (4)

Drer\me3

12 of 15

Yes

Drer\me1

10 of 15

Yes

Drer\me2

10 of 15

Drer\LOC101882012

4 of 15

Yes

Caenorhabditis elegans (Nematode, roundworm) (1)

Cele\men-1

12 of 15

Yes

Arabidopsis thaliana (thale-cress) (6)

Atha\NADP-ME4

9 of 9

Yes

Atha\NADP-ME1

8 of 9

Atha\NADP-ME2

8 of 9

Yes

Atha\NADP-ME3

8 of 9

Yes

Atha\NAD-ME1

2 of 9

Yes

Atha\NAD-ME2

2 of 9

Yes

Saccharomyces cerevisiae (Brewer's yeast) (1)

Scer\MAE1

12 of 15

Yes

Schizosaccharomyces pombe (Fission yeast) (2)

Spom\mae2

9 of 12

Yes

Spom\SPAC750.08c

3 of 12

Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091903KG )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila suzukii

Spotted wing Drosophila

Dsuz\DS10_00010574

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA10087

Drosophila persimilis

Dper\GL23167

Drosophila willistoni

Dwil\GK10867

Drosophila virilis

Dvir\GJ10817

Drosophila mojavensis

Dmoj\GI24244

Drosophila grimshawi

Dgri\GH19210

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091502WC )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17021469

Musca domestica

House fly

Mdoa\17020809

Glossina morsitans

Tsetse fly

Gmor\GMOY004146

Glossina morsitans

Tsetse fly

Gmor\GMOY007044

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_00416

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_03813

Mayetiola destructor

Hessian fly

Mdes\Mdes000426

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL005790

Anopheles darlingi

American malaria mosquito

Adar\ADAC010391

Anopheles gambiae

Malaria mosquito

Agam\AGAP000184

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ012341

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W02NP )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA006247

Bombyx mori

Silkmoth

Bmor\BGIBMGA006246

Bombyx mori

Silkmoth

Bmor\BGIBMGA006419

Danaus plexippus

Monarch butterfly

Dple\DPOGS211597-PA

Danaus plexippus

Monarch butterfly

Dple\DPOGS208229-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL008370

Heliconius melpomene

Postman butterfly

Hmel\HMEL007906

Apis florea

Little honeybee

Aflo\2711705668

Apis florea

Little honeybee

Aflo\2181413332

Apis mellifera

Western honey bee

Amel\GB55096

Apis mellifera

Western honey bee

Amel\Mdh

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP14952

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP19417

Bombus terrestris

Buff-tailed bumblebee

Bter\15211055

Bombus terrestris

Buff-tailed bumblebee

Bter\15205506

Linepithema humile

Argentine ant

Lhum\LH13502

Linepithema humile

Argentine ant

Lhum\LH23125

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:00241d

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:002049

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG013704

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG000998

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_00923

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_08696

Tribolium castaneum

Red flour beetle

Tcas\TC005233

Tribolium castaneum

Red flour beetle

Tcas\TC005232

Pediculus humanus

Human body louse

Phum\PHUM268250

Rhodnius prolixus

Kissing bug

Rpro\RPRC011604

Rhodnius prolixus

Kissing bug

Rpro\RPRC008698

Cimex lectularius

Bed bug

Clec\CLEC013061

Cimex lectularius

Bed bug

Clec\CLEC012371

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI004126

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI006059

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_15737

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X02XF )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR013263

Strigamia maritima

European centipede

Smar\SMAR006647

Ixodes scapularis

Black-legged tick

Isca\ISCW011260

Stegodyphus mimosarum

African social velvet spider

Smim\KFM76723.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur09g00240

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_304467

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G04H9 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Me1

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Me3

Gallus gallus

Domestic chicken

Ggal\ME1

Gallus gallus

Domestic chicken

Ggal\ME3

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (4)

Gene Symbol

Score

Source

Alignment

9 of 10

7 of 10

5 of 10

5 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 2 )

Allele

Disease

Interaction

References

Men^KK107809

ameliorates myofibrillar myopathy 2

modeled by Hsap\CRYAB^R120G.UAS

(Xie et al., 2013)

Men^GL01234

ameliorates cancer

modeled by brat^HMS01121

(Hussain et al., 2017)

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

15 of 15

12 of 15

9 of 15

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Men - Akap200

experimental knowledge based

(Guruharsha et al., 2011)

Men - Cndp2

experimental knowledge based

(Guruharsha et al., 2011)

Men - Fim

experimental knowledge based

(Guruharsha et al., 2011)

Men - Moe

experimental knowledge based

(Guruharsha et al., 2011)

Men - Syb

experimental knowledge based

(Guruharsha et al., 2011)

Men - capt

experimental knowledge based

(Guruharsha et al., 2011)

Men - osk

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Hurd et al., 2016)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

brat

suppressible

Men

(Hussain et al., 2017)

Dronc

enhanceable

Men

(Yang et al., 2010)

enhanceable

Men

(Müller et al., 2005)

pbl

suppressible

Men

(Gregory et al., 2007)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

70650

DroID - A comprehensive database of gene and protein interactions.

FBgn0002719

MIST (protein-protein) - An integrated Molecular Interaction Database

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel

gluconeogenesis I

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0002719

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-52

Cytogenetic map

87C6-87C7

Sequence location

3R:12,713,103..12,722,442 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

87C6-87C7

Limits computationally determined from genome sequence between P{lacW}Vha55^j2E9 and P{PZ}CtBP⁰³⁴⁶³

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

87D1-87D1

(Cordes, 1999.9.20, Farkas, 1999.12.20)

Experimentally Determined Recombination Data

Location

3-52

(FlyBase, 2016)

3-50.0

(Comeron et al., 2012)

3-53.1

(Clark et al., 1995)

(Choudhary et al., 1992)

3-53.1 +/- 0.6

(Franklin and Rumball, 1971)

3-51.73

Left of (cM)

Right of (cM)

Notes

Based on 91 recombinants between kar and ry.

Stocks and Reagents

Stocks (36)

Bloomington

12824

w¹¹¹⁸; P{GT1}Men^BG02790

4025

Men^nNC3 Aldox89A^nNC4/TM2

Kyoto

101689

Men^nNC1 / TM6

101690

Men^nNC2 / TM6

113281

w^*; P{GawB}Men^NP3447

123240

w¹¹¹⁸; P{RS3}Men^CB-0569-3

124110

w¹¹¹⁸; P{RS3}Men^CB-6459-3

124328

w¹¹¹⁸; P{RS3}Men^UM-8082-3

125016

w¹¹¹⁸; P{RS5}Men^5-HA-1025

125309

w¹¹¹⁸; P{RS5}Men^5-HA-1631

VDRC

v330428

P{VSH330428}attP40

v104016

P{KK107809}VIE-260B

More stocks available...

Genomic Clones (18)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (152)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

LD27718

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0002719

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0002719

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

47173

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for database merge of

Source for merge of: Men CG10120

(Bayraktaroglu, 2000.8.7)

Source for merge of: Men anon-WO0118547.278

(FlyBase, 1996-)

Additional comments

"Men" may correspond to "Scim32". Sequence analysis off ends of P{SUPor-P} in Scim insertion mutant places "Scim32" near/in "Men".

(Dobie and Karpen, 2001.4.18)

Source for merge of Men anon-WO0118547.278 was sequence comparison ( date:051113 ).

(FlyBase, 1996-)

Other Comments

Area matching Drosophila EST AA567704.

(Davies, 2001.3.15)

The effects of a high sucrose diet on live weight, total protein, stored lipid and glycogen and crude activities of 12 enzymes involved in energy metabolism are quantified. The activities of many enzymes are reduced by the sucrose treatment.

(Wang and Clark, 1995)

Gramoxone has no mutagenic effect on the genetic background of Men.

(el Abidin Salam et al., 1993)

Presence of an extra dose of the region from 8D10-12 to 9A1-2 results in an approximately 25% increase in Men activity.

(Williamson and Bentley, 1983)

All null alleles tested survive and are fertile in combination with Df(3R)ry31, which lacks Men⁺.

(Voelker et al., 1980)

The NADPH/NADP ratio in null genotypes is one-seventh that of wild type.

(Geer et al., 1979)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 151 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

47173

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0002719

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0002719

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

47173/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0002719

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Men_1
Men_2

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0002719

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0002719

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG10120

Flygut - An atlas of the Drosophila adult midgut

FBgn0002719

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0002719

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

47173

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG10120

MARRVEL_MODEL - MARRVEL (model organism gene)

47173

modMine - A data warehouse for the modENCODE project

FBgn0002719

Linkouts

BioGRID - A database of protein and genetic interactions.

70650

DroID - A comprehensive database of gene and protein interactions.

FBgn0002719

DRSC - Results frm RNAi screens

FBgn0002719

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0002719

FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel

gluconeogenesis I

FlyMine - An integrated database for Drosophila genomics

FBgn0002719

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

MIST (protein-protein) - An integrated Molecular Interaction Database

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Synonyms and Secondary IDs (16)

Reported As

Symbol Synonym

CG10120

(Meadows, 2017.9.14, Hurd et al., 2016, Choo et al., 2015, Kim et al., 2015, Cammarato et al., 2011, O'Keefe et al., 2011, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Rach et al., 2009, Puri et al., 2008, Gregory et al., 2007, Lai et al., 2007, Toledano-Katchalski et al., 2007, Pile et al., 2003, Seegmiller et al., 2002, Dobie and Karpen, 2001.4.18)

MDH

(Farkas and Knopp, 1998)

(Deckert-Cruz et al., 2004, Montooth et al., 2003, Bondinas et al., 2002, Farkas et al., 2002, Bondinas et al., 2001, Harshman et al., 1999, Clark and Fucito, 1998, Clark and Wang, 1997, Clark et al., 1995, Wang and Clark, 1995, Clark, 1994, Clark and Wang, 1994, Choudhary et al., 1992, Clark, 1990, Clark et al., 1990)

ME1

(Parkhitko et al., 2020)

MEN

(Merritt et al., 2009)

Mdh-NADP

(Franklin and Rumball, 1971)

(Zapata et al., 2002, Harshman and Schmid, 1998, Matthews and Munstermann, 1994, el Abidin Salam et al., 1993)

Men

(Cattenoz et al., 2021, Ågren et al., 2020, Cho et al., 2020, Jevitt et al., 2020, Lathen et al., 2020, Parkhitko et al., 2020, Bradshaw, 2019, Engel et al., 2019, Everman et al., 2019, Ge et al., 2019, Gene Disruption Project members, 2018-, Garlapow et al., 2017, Fear et al., 2016, Kemppainen et al., 2016, Kockel et al., 2016, Wani et al., 2016, Gene Disruption Project members, 2015-, Grotewiel and Bettinger, 2015, Kim et al., 2015, Talbert et al., 2015, Lavington et al., 2014, Beckett et al., 2013, Short and Lazzaro, 2013, Xie et al., 2013, Izutsu et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Morozova et al., 2012, Rzezniczak et al., 2012, Lum and Merritt, 2011, O'Keefe et al., 2011, Kong et al., 2010, Wasbrough et al., 2010, Yang et al., 2010, Morozova et al., 2009, Franciscovich et al., 2008, Puri et al., 2008, Buszczak et al., 2007, Morozova et al., 2007, Haynes et al., 2006, Reeves and Posakony, 2005, Farkas et al., 2002)

anon-WO0118547.278

mem

(Seugnet et al., 2009)

men

(Lebo et al., 2021, Ramond et al., 2020, Haines and Eisen, 2018, Paik et al., 2012, Rach et al., 2009, Harvey et al., 2008)

Name Synonyms

Malic enzyme

(Garlapow et al., 2017, Cammarato et al., 2011, Lum and Merritt, 2011, Barnes et al., 2010, Pedersen et al., 2010, Robinson et al., 2010, Morozova et al., 2009, Seugnet et al., 2009, Harbison and Sehgal, 2008, Morozova et al., 2007, Deckert-Cruz et al., 2004)

NADP-dependent malate dehydrogenase

(Paik et al., 2012)

NADP-malic enzyme

(Bowen and Williamson, 1994, Wegulo and Williamson, 1994)

malic enzyme

(Kemppainen et al., 2016, Kim et al., 2015, O'Keefe et al., 2011, Yang et al., 2010, Harbison and Sehgal, 2008, Morozova et al., 2006, Montooth et al., 2003)

malic enzyme (cytosolic)

(Talbert et al., 2015)

Secondary FlyBase IDs

FBgn0038081
FBgn0062124

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (174)

Report Sections

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