Gene Dmel\Men
| General Information | ||||
|---|---|---|---|---|
| Symbol | Dmel\Men | Species | D. melanogaster | |
| Name | Malic enzyme | Annotation symbol | CG10120 | |
| Feature type | protein_coding_gene | FlyBase ID | FBgn0002719 | |
| Created / Updated | 2003-12-01/2003-12-01 | |||
| Genomic Location | ||||
| Chromosome (arm) | 3R | Recombination map | 3-51.73 | |
| Cytogenetic map | 87C6-87C7 | Sequence location | 3R:8,538,819..8,548,267 [-] | |
| Map ( GBrowse ) |
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Summary Information
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Automatically generated summary
See sections below for more information | The gene Malic enzyme is referred to in FlyBase by the symbol Men (CG10120, FBgn0002719). It has the cytological map location 87C6-87C7. Its sequence location is 3R:8538819..8548267. Its molecular function is described as: malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) activity; NAD binding. It is involved in the biological process malate metabolic process. 42 alleles are reported. The phenotypes of these alleles are annotated with: germ cell; eye. It has 2 annotated transcripts and 2 annotated polypeptides. | |||
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
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| Gene/Allele symbols may differ from current usage | Men: Malic enzyme
Structural gene for malic enzyme = (S)-Malate: NADP+
oxidoreductase [MEN (EC 1.1.1.40)], a tetramer with subunit
molecular weight of 58,000 (Lee, Langley, and Burkhart, 1978,
Anal. Biochem. 86: 697-296) or 67,250 (Geer, Krochko, Oliver,
Walker, and Williamson, 1980, Comp. Biochem. Physiol.
65B: 25-34). Biochemical characterization by Geer, et al.,
(1980). Enzyme known to provide NADP for lipogenesis; levels
in larvae increased by dietary carbohydrate and decreased by
dietary lipid. Levels normally high in early third-instar
larvae, reducing to half in late third instar, rising again in
ageing adults. Highest specific activity found in larval fat
body and, among cellular fractions, in the cytosol (Geer, Krochko, and Williamson, 1979, Biochem. Genet. 17: 867-79).
Histochemical staining of larval imaginal discs reveals enzyme
activity localized to nervous elements, including ommatidial
precursors, the morphogenetic furrow, and the optic nerve in
the eye antennal disc; and the chordotonal organ and the nerve
traversing the leg discs; wing, haltere, labial, and genital
discs unstained; however genital disc derivatives, the ejaculatory duct and paragonia in males and oviducts of females are
stained in adults (Finkbohner, Cunningham, and Kuhn, 1985,
Wilhelm Roux's Arch. Dev. Biol. 194: 217-23). Staining of
morphogenetic groove absent in D. simulans and in simulans-melanogaster hybrids except when such hybrids contain two
copies of 3R from D. melanogaster and none from D. simulans
(Kuhn and Sprey, 1987, Genetics 115: 277-81). All null
alleles tested survive and are fertile in combination with
Df(3R)ry3l, which lacks Men+ (Voelker et al.). The NADPH/NADP
ratio in such null genotypes is one-seventh that of wild type
(Geer et al., 1979). Presence of an extra dose of the region
from 8D10-12 to 9A1-2 results in an approximately 25% increase
in MEN activity (Williamson and Bentley, 1983, Genetics
103: 649-58).
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Detailed Mapping Data
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| FlyBase Computed Cytological Location | ||||
Cytogenetic map Evidence for location 87C6-87C7
Limits computationally determined from genome sequence between P{lacW}Vha55j2E9 and P{PZ}CtBP03463
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| Experimentally Determined Cytological Location | ||||
Cytogenetic map Notes References 87D1-87D1 87D1-87D1 | ||||
| Experimentally Determined Recombination Data | ||||
| Location | 3-51.73 3-53.1 +/- 0.6 3-53.1 | |||
| Left of (cM) | ||||
| Right of (cM) | ||||
| Notes | ||||
| Molecular Map Data | ||||
Gene Order (in direction of increasing cytology)
References Gene Order (overall orientation not stated) References Overall orientation not stated: mus308+ Men- | ||||
Gene Model & Products
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Please see the
GBrowse view of
Dmel\Men
for information on other features
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| Comments on Gene Model | ||||
Transcript Data
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| Annotated Transcripts | ||||
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) | ||||
| Additional Transcript Data & Comments | ||||
| Reported size (kB) | ||||
| Comments | ||||
| External Data | ||||
| Crossreferences | ||||
Polypeptide Data
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| Annotated Polypeptides | ||||
Name FlyBase ID
Predicted MW (kD)
Length (aa)
Theoretical pI
RefSeq ID
GenBank protein
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| Additional Polypeptide Data & Comments | ||||
| Reported size (kD) | ||||
| Comments | ||||
| External Data | ||||
| Linkouts | PANTHER
- Protein classification by function, families, and pathways
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| Crossreferences | InterPro
domains - A database of protein families, domains, and functional sites
• Malic oxidoreductase (IPR001891)
Malic enzyme, N-terminal (IPR012301)
Malic enzyme, NAD-binding (IPR012302)
Malic enzyme, conserved site (IPR015884)
NAD(P)-binding (IPR016040)
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Sequences Consistent with the Gene Model
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| DDBJ
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EMBL / GenBank | DNA sequence Protein sequence Name | |||
| UniProtKB/Swiss-Prot | ||||
| UniProtKB/TrEMBL | ||||
| Maps to | ||||
| Does NOT map to | ||||
| Identified with | ||||
Mapped Features & Mutations
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Please see
GBrowse
or insertion reports for information on insertions of transgenic
constructs and features not listed here
Type Symbol & Location Additional Notes References | ||||
External Data
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| Linkouts | DEDB
- Drosophila exon database: splicing graphs
• | |||
| Crossreferences | ||||
Expression Data
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| FlyBase-Curated Data | ||||
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Transcript and Protein data | Please see the FlyBase Gene Expression Report for details of gene expression from the literature. | |||
Summary of Transcript Expression
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Stage Tissue/Position
Reference
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| Marker for | ||||
| Subcellular Localization | ||||
| CV Term | ||||
Summary of Polypeptide Expression
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Stage Tissue/Position
Reference
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| Marker for | ||||
| Subcellular Localization | ||||
| CV Term | ||||
Microarray Data
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Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002)
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External Data & Images
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| Linkouts | FLIGHT
- Cell culture data for RNAi and other high-throughput technologies
FlyAtlas
- Adult expression by tissue, using Affymetrix Dros2 array
GEO (NCBI)
- Gene expression data: microarray and other high-throughput technologies
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Alleles & Phenotypes
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Summary of Allele Phenotypes
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Lethality Allele Other Phenotypes Allele | ||||


