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Gene Dmel\Men

General Information
Symbol	Dmel\Men	Species	D. melanogaster
Name	Malic enzyme	Annotation symbol	CG10120
Feature type	protein_coding_gene	FlyBase ID	FBgn0002719
Gene Model Status	Current	Stock availability	33 publicly available
Also Known As	ME
Genomic Location
Chromosome (arm)	3R	Recombination map	3-51.73
Cytogenetic map	87C6-87C7	Sequence location	3R:8,538,825..8,548,267 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Malic enzyme is referred to in FlyBase by the symbol Dmel\Men (CG10120, FBgn0002719). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) activity. There is experimental evidence that it is involved in the biological process: regulation of cell death. 67 alleles are reported. The phenotype of these alleles is annotated with: eye. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: Malic enzyme, N-terminal; Malic enzyme, NAD-binding; Malic enzyme, conserved site; Malic oxidoreductase; NAD(P)-binding domain. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of moderate expression. Peak expression observed within 12-24 hour embryonic stages, during early larval stages, during late pupal stages, in adult male stages. Summary of FlyAtlas Anatomical Expression Data: Many larval and adult organs/tissues expressed at high levels. Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, adult fat body, adult spermathecae, adult testis, larval/adult carcass. Expression at moderate levels in the following post-embryonic organs or tissues: adult brain, larval fat body, larval trachea, adult ovary, adult male accessory gland. Comments on Affy2 ProbeSet: ProbeSet 1629095_a_at completely aligns to an exonic region common to each of the 2 FlyBase-annotated transcript isoforms of Men. Gene sequence location is 3R:8538825..8548267.
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	Men: Malic enzyme Structural gene for malic enzyme = (S)-Malate: NADP+ oxidoreductase [MEN (EC 1.1.1.40)], a tetramer with subunit molecular weight of 58,000 (Lee, Langley, and Burkhart, 1978, Anal. Biochem. 86: 697-296) or 67,250 (Geer, Krochko, Oliver, Walker, and Williamson, 1980, Comp. Biochem. Physiol. 65B: 25-34). Biochemical characterization by Geer, et al., (1980). Enzyme known to provide NADP for lipogenesis; levels in larvae increased by dietary carbohydrate and decreased by dietary lipid. Levels normally high in early third-instar larvae, reducing to half in late third instar, rising again in ageing adults. Highest specific activity found in larval fat body and, among cellular fractions, in the cytosol (Geer, Krochko, and Williamson, 1979, Biochem. Genet. 17: 867-79). Histochemical staining of larval imaginal discs reveals enzyme activity localized to nervous elements, including ommatidial precursors, the morphogenetic furrow, and the optic nerve in the eye antennal disc; and the chordotonal organ and the nerve traversing the leg discs; wing, haltere, labial, and genital discs unstained; however genital disc derivatives, the ejaculatory duct and paragonia in males and oviducts of females are stained in adults (Finkbohner, Cunningham, and Kuhn, 1985, Wilhelm Roux's Arch. Dev. Biol. 194: 217-23). Staining of morphogenetic groove absent in D. simulans and in simulans-melanogaster hybrids except when such hybrids contain two copies of 3R from D. melanogaster and none from D. simulans (Kuhn and Sprey, 1987, Genetics 115: 277-81). All null alleles tested survive and are fertile in combination with Df(3R)ry3l, which lacks Men+ (Voelker et al.). The NADPH/NADP ratio in such null genotypes is one-seventh that of wild type (Geer et al., 1979). Presence of an extra dose of the region from 8D10-12 to 9A1-2 results in an approximately 25% increase in MEN activity (Williamson and Bentley, 1983, Genetics 103: 649-58).
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2011_10	Controlled Vocabulary Terms day 42 of adulthood day 41 of adulthood day 30 of adulthood day 49 of adulthood day 17 of adulthood day 45 of adulthood day 6 of adulthood day 5 of adulthood day 8 of adulthood day 26 of adulthood day 21 of adulthood day 13 of adulthood day 25 of adulthood day 22 of adulthood day 20 of adulthood day 39 of adulthood day 7 of adulthood day 36 of adulthood day 11 of adulthood day 37 of adulthood day 27 of adulthood day 18 of adulthood day 19 of adulthood day 28 of adulthood day 9 of adulthood day 29 of adulthood adult heart day 40 of adulthood day 16 of adulthood day 24 of adulthood day 15 of adulthood day 33 of adulthood day 43 of adulthood day 47 of adulthood day 38 of adulthood day 32 of adulthood day 48 of adulthood day 35 of adulthood day 23 of adulthood day 12 of adulthood day 10 of adulthood day 31 of adulthood spermatozoon day 34 of adulthood day 44 of adulthood day 14 of adulthood male day 46 of adulthood References Knowles-Barley, 2011.8.24, BrainTrap expression curation.
FB2012_01	Transgenic Constructs P{UAS-Men.dsRNA} Controlled Vocabulary Terms regulation of cell death Alleles Men[dsRNA.Scer\UAS] References Lum and Merritt, 2011, Genetics 189(3): 837--849 Yang et al., 2010, EMBO J. 29(18): 3196--3207 Sequence features BKN27809 HFA16738 HFA12924
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 87C6-87C7   Limits computationally determined from genome sequence between P{lacW}Vha55j2E9 and P{PZ}CtBP03463
Experimentally Determined Cytological Location
Cytogenetic map Notes References 87D1-87D1     (Cordes, 1999.9.20) 87D1-87D1     (Farkas, 1999.12.20)
Experimentally Determined Recombination Data
Location	3-51.73   3- (Choudhary et al., 1992) 3-53.1 +/- 0.6 (Franklin and Rumball, 1971) 3-53.1 (Clark et al., 1995)
Left of (cM)	ry
Right of (cM)	kar
Notes	Based on 91 recombinants between kar and ry.
Gene Model & Products
Please see the GBrowse view of Dmel\Men for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.39
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Men-RA FBtr0082661  NM_169479   2767   759 Men-RB FBtr0082660  NM_080141   3009   763
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Men-PA   FBpp0082130   84.4   759   7.23     NP_731739   AAF54860 Men-PB   FBpp0082129   84.6   763   6.60     NP_524880   AAF54859
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Malic oxidoreductase (IPR001891) Malic enzyme, N-terminal (IPR012301) Malic enzyme, NAD-binding (IPR012302) Malic enzyme, conserved site (IPR015884) NAD(P)-binding domain (IPR016040)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA567704   AA948992   AE014297 AAF54859   AE014297 AAF54860   AF187998 AAF43601   AF187999 AAF43602   AF188000 AAF43603   AI062582   AI109241   AI113348   AI258186   AI258979   AI295700   AI295757   AI297942   AI389417   AI389423   AI402720   AI403673   AI403683   AI404174   AI404880   AI531341   AI531667   AI532234   AI532327   AI534418   AI945435   AI946400   AJ251545 CAB64262   AQ025061   AQ025071   AQ025097   AQ025100   AQ025102   AQ025207   AQ025272   AQ073955   AQ254603   AW942773   AX094154   AY118540 AAM49909   BE977088   BF486099   BF497876   BG634378   BG635622   BG636825   BG637240   BH146183   BI162699   BI162700   BI163214   BI168057   BI173467   BI215699   BI228328   BI237258   BI238418   BI357492   BI364358   BI365944   BI367939   BI376177   BI485654   BI487259   BI565869   BI568756   BI571310   BI571717   BI572078   BI572770   BI572820   BI572857   BI579552   BI582896   BI583170   BI583429   BI583648   BI584259   BI584435   BI586976   BI590953   BI592504   BI604742   BI604779   BI604785   BI607974   BI616205   BI619410   BI619622   BI619974   BI622425   BI624051   BI628936   BI630038   BI633602   BI634064   BI634071   BI635235   BI638237   BI639359   BI640199   BI640335   BI641489   BI641961   BP541856   BP555610   BP555611   BP559071   BT100054   CA804977   CO280374   CO301536   CO313245   CO317916   CO339316   CO343718   CZ467108   CZ472165   CZ472166   CZ475708   CZ477499   DQ148977 ABA19464   DQ148978 ABA19465   DQ148979 ABA19466   DQ148980 ABA19467   DQ148981 ABA19468   DQ148982 ABA19469   DQ148983 ABA19470   DQ148984 ABA19471   DQ148985 ABA19472   DQ148986 ABA19473   DQ148987 ABA19474   DQ148988 ABA19475   DQ148989 ABA19476   DQ148990 ABA19477   DQ148991 ABA19478   DQ148992 ABA19479   DQ148993 ABA19480   DQ148994 ABA19481   DQ148995 ABA19482   DQ148996 ABA19483   DQ148997 ABA19484   DQ148998 ABA19485   DQ148999 ABA19486   DQ149000 ABA19487   DQ149001 ABA19488   DQ149002 ABA19489   DQ149003 ABA19490   DQ149004 ABA19491   DQ149005 ABA19492   DQ149006 ABA19493   DQ149007 ABA19494   DQ149008 ABA19495   DQ149009 ABA19496   EC057322   EC073301   EC217344   EC234716   EC238712   EC256714   ES689057   EV575696   EV576787   EV578028   EV586220   EV589751   EV590731   EV592636   EV593761   EV599741   FE862623   FE862651   FE862678   FE862712   FE862734   FE863163   FE863230   FE863257   FE863294   GH738943   GH741703   GH742922   GH743330   GH743657   GH744039   GH744443   GH744496   GH744998   GH745512   GH745515   GH745832   GH746822   GH761542   GH762113   GH762218   GH762687   GH763515   GH763846   GH764619   GH764780   GH764916   GH765513   GH765801   GH765933   GH766285   GH767226   GH768033   GH769562   GH769900   GO254440   GO254525
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q2QBM1 Q9NIW0 Q9NIW1 Q9NIW2 Q9U1J1 Q9VG31 Q9VG32
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
mass spectroscopy Stage Tissue/Position (including subcellular localization) Reference day 5 of adulthood & male -- day 7 of adulthood & male   spermatozoon   (Wasbrough et al., 2010) day 7 of adulthood -- day 49 of adulthood   adult heart   (Cammarato et al., 2011)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0002719    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0002719 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of moderate expression. Peak expression observed within 12-24 hour embryonic stages, during early larval stages, during late pupal stages, in adult male stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0002719 expression level Developmental Stage   Expression Level embryo 00-02hr    4495 embryo 02-04hr    648 embryo 04-06hr    717 embryo 06-08hr    4288 embryo 08-10hr    5537 embryo 10-12hr    6048 embryo 12-14hr    4525 embryo 14-16hr    7021 embryo 16-18hr    10287 embryo 18-20hr    17634 embryo 20-22hr    33949 embryo 22-24hr    35037 larva L1    20982 larva L2    18905 larva L3 12hr old    18903 larva L3 puffstage 1-2    7877 larva L3 puffstage 3-6    3416 larva L3 puffstage 7-9    2090 white prepupae new    5730 white prepupae 12hr    5938 white prepupae 24hr    4782 pupae 2d postWPP    5455 pupae 3d postWPP    4959 pupae 4d postWPP    12642 adult male 01day    13704 adult male 05day    14838 adult male 30day    14926 adult female 01day    7960 adult female 05day    8702 adult female 30day    9554 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (4495) embryo 02-04hr  (648) embryo 04-06hr  (717) embryo 06-08hr  (4288) embryo 08-10hr  (5537) embryo 10-12hr  (6048) embryo 12-14hr  (4525) embryo 14-16hr  (7021) embryo 16-18hr  (10287) embryo 18-20hr  (17634) embryo 20-22hr  (33949) embryo 22-24hr  (35037) larva L1  (20982) larva L2  (18905) larva L3 12hr old  (18903) larva L3 puffstage 1-2  (7877) larva L3 puffstage 3-6  (3416) larva L3 puffstage 7-9  (2090) white prepupae new  (5730) white prepupae 12hr  (5938) white prepupae 24hr  (4782) pupae 2d postWPP  (5455) pupae 3d postWPP  (4959) pupae 4d postWPP  (12642) adult male 01day  (13704) adult male 05day  (14838) adult male 30day  (14926) adult female 01day  (7960) adult female 05day  (8702) adult female 30day  (9554) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (4495) embryo 02-04hr    648 embryo 04-06hr    717 embryo 06-08hr  (4288) embryo 08-10hr  (5537) embryo 10-12hr  (6048) embryo 12-14hr  (4525) embryo 14-16hr  (7021) embryo 16-18hr  (10287) embryo 18-20hr  (17634) embryo 20-22hr  (33949) embryo 22-24hr  (35037) larva L1  (20982) larva L2  (18905) larva L3 12hr old  (18903) larva L3 puffstage 1-2  (7877) larva L3 puffstage 3-6  (3416) larva L3 puffstage 7-9  (2090) white prepupae new  (5730) white prepupae 12hr  (5938) white prepupae 24hr  (4782) pupae 2d postWPP  (5455) pupae 3d postWPP  (4959) pupae 4d postWPP  (12642) adult male 01day  (13704) adult male 05day  (14838) adult male 30day  (14926) adult female 01day  (7960) adult female 05day  (8702) adult female 30day  (9554) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    4495 embryo 02-04hr    648 embryo 04-06hr    717 embryo 06-08hr    4288 embryo 08-10hr    5537 embryo 10-12hr    6048 embryo 12-14hr    4525 embryo 14-16hr    7021 embryo 16-18hr  10287 embryo 18-20hr  (17634) embryo 20-22hr  (33949) embryo 22-24hr  (35037) larva L1  (20982) larva L2  (18905) larva L3 12hr old  (18903) larva L3 puffstage 1-2    7877 larva L3 puffstage 3-6    3416 larva L3 puffstage 7-9    2090 white prepupae new    5730 white prepupae 12hr    5938 white prepupae 24hr    4782 pupae 2d postWPP    5455 pupae 3d postWPP    4959 pupae 4d postWPP  (12642) adult male 01day  (13704) adult male 05day  (14838) adult male 30day  (14926) adult female 01day    7960 adult female 05day    8702 adult female 30day    9554 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    4495 embryo 02-04hr    648 embryo 04-06hr    717 embryo 06-08hr    4288 embryo 08-10hr    5537 embryo 10-12hr    6048 embryo 12-14hr    4525 embryo 14-16hr    7021 embryo 16-18hr    10287 embryo 18-20hr    17634 embryo 20-22hr    33949 embryo 22-24hr    35037 larva L1    20982 larva L2    18905 larva L3 12hr old    18903 larva L3 puffstage 1-2    7877 larva L3 puffstage 3-6    3416 larva L3 puffstage 7-9    2090 white prepupae new    5730 white prepupae 12hr    5938 white prepupae 24hr    4782 pupae 2d postWPP    5455 pupae 3d postWPP    4959 pupae 4d postWPP    12642 adult male 01day    13704 adult male 05day    14838 adult male 30day    14926 adult female 01day    7960 adult female 05day    8702 adult female 30day    9554 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0002719 expression level Developmental Stage   Expression Level embryo 00-02hr    4495 embryo 02-04hr    648 embryo 04-06hr    717 embryo 06-08hr    4288 embryo 08-10hr    5537 embryo 10-12hr    6048 embryo 12-14hr    4525 embryo 14-16hr    7021 embryo 16-18hr    10287 embryo 18-20hr    17634 embryo 20-22hr    33949 embryo 22-24hr    35037 larva L1    20982 larva L2    18905 larva L3 12hr old    18903 larva L3 puffstage 1-2    7877 larva L3 puffstage 3-6    3416 larva L3 puffstage 7-9    2090 white prepupae new    5730 white prepupae 12hr    5938 white prepupae 24hr    4782 pupae 2d postWPP    5455 pupae 3d postWPP    4959 pupae 4d postWPP    12642 adult male 01day    13704 adult male 05day    14838 adult male 30day    14926 adult female 01day    7960 adult female 05day    8702 adult female 30day    9554 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (4495) embryo 02-04hr  (648) embryo 04-06hr  (717) embryo 06-08hr  (4288) embryo 08-10hr  (5537) embryo 10-12hr  (6048) embryo 12-14hr  (4525) embryo 14-16hr  (7021) embryo 16-18hr  (10287) embryo 18-20hr  (17634) embryo 20-22hr  (33949) embryo 22-24hr  (35037) larva L1  (20982) larva L2  (18905) larva L3 12hr old  (18903) larva L3 puffstage 1-2  (7877) larva L3 puffstage 3-6  (3416) larva L3 puffstage 7-9  (2090) white prepupae new  (5730) white prepupae 12hr  (5938) white prepupae 24hr  (4782) pupae 2d postWPP  (5455) pupae 3d postWPP  (4959) pupae 4d postWPP  (12642) adult male 01day  (13704) adult male 05day  (14838) adult male 30day  (14926) adult female 01day  (7960) adult female 05day  (8702) adult female 30day  (9554) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (4495) embryo 02-04hr    648 embryo 04-06hr    717 embryo 06-08hr  (4288) embryo 08-10hr  (5537) embryo 10-12hr  (6048) embryo 12-14hr  (4525) embryo 14-16hr  (7021) embryo 16-18hr  (10287) embryo 18-20hr  (17634) embryo 20-22hr  (33949) embryo 22-24hr  (35037) larva L1  (20982) larva L2  (18905) larva L3 12hr old  (18903) larva L3 puffstage 1-2  (7877) larva L3 puffstage 3-6  (3416) larva L3 puffstage 7-9  2090 white prepupae new  (5730) white prepupae 12hr  (5938) white prepupae 24hr  (4782) pupae 2d postWPP  (5455) pupae 3d postWPP  (4959) pupae 4d postWPP  (12642) adult male 01day  (13704) adult male 05day  (14838) adult male 30day  (14926) adult female 01day  (7960) adult female 05day  (8702) adult female 30day  (9554) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    4495 embryo 02-04hr    648 embryo 04-06hr    717 embryo 06-08hr    4288 embryo 08-10hr    5537 embryo 10-12hr    6048 embryo 12-14hr    4525 embryo 14-16hr    7021 embryo 16-18hr  10287 embryo 18-20hr  17634 embryo 20-22hr  (33949) embryo 22-24hr  (35037) larva L1  (20982) larva L2  18905 larva L3 12hr old  18903 larva L3 puffstage 1-2    7877 larva L3 puffstage 3-6    3416 larva L3 puffstage 7-9    2090 white prepupae new    5730 white prepupae 12hr    5938 white prepupae 24hr    4782 pupae 2d postWPP    5455 pupae 3d postWPP    4959 pupae 4d postWPP  12642 adult male 01day  13704 adult male 05day  14838 adult male 30day  14926 adult female 01day    7960 adult female 05day    8702 adult female 30day    9554 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    4495 embryo 02-04hr    648 embryo 04-06hr    717 embryo 06-08hr    4288 embryo 08-10hr    5537 embryo 10-12hr    6048 embryo 12-14hr    4525 embryo 14-16hr    7021 embryo 16-18hr    10287 embryo 18-20hr    17634 embryo 20-22hr    33949 embryo 22-24hr    35037 larva L1    20982 larva L2    18905 larva L3 12hr old    18903 larva L3 puffstage 1-2    7877 larva L3 puffstage 3-6    3416 larva L3 puffstage 7-9    2090 white prepupae new    5730 white prepupae 12hr    5938 white prepupae 24hr    4782 pupae 2d postWPP    5455 pupae 3d postWPP    4959 pupae 4d postWPP    12642 adult male 01day    13704 adult male 05day    14838 adult male 30day    14926 adult female 01day    7960 adult female 05day    8702 adult female 30day    9554 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0002719    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0002719 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Many larval and adult organs/tissues expressed at high levels. Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, adult fat body, adult spermathecae, adult testis, larval/adult carcass. Expression at moderate levels in the following post-embryonic organs or tissues: adult brain, larval fat body, larval trachea, adult ovary, adult male accessory gland. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0002719 expression level Tissue   Expression Level Larval Central Nervous System    672.15 Larval Midgut    734.9 Larval Hindgut    664.4 Larval Malpighian Tubules    1087.9 Larval Fat Body    440 Larval Salivary Gland no informative data Larval Trachea    216.25 Larval Carcass    762.4 Adult Head    1514.1 Adult Eye    868.3 Adult Brain    456.2 Adult Thoracic-Abdominal Ganglion    557.7 Adult Crop    2003.7 Adult Midgut    545.1 Adult Hindgut    945.7 Adult Malpighian Tubules    1977.3 Adult Fat Body    3827.3 Adult Salivary Gland no informative data Adult Heart    2915.3 Adult VirginFemale Spermatheca    4206.5 Adult InseminatedFemale Spermatheca    2897.6 Adult Ovary    479.6 Adult Testis    785.1 Adult Male Accessory Gland    276.1 Adult Carcass    2830.2 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  (672.15) Larval Midgut  (734.9) Larval Hindgut  (664.4) Larval Malpighian Tubules  (1087.9) Larval Fat Body    440 Larval Salivary Gland no informative data Larval Trachea    216.25 Larval Carcass  (762.4) Adult Head  (1514.1) Adult Eye  (868.3) Adult Brain    456.2 Adult Thoracic-Abdominal Ganglion  (557.7) Adult Crop  (2003.7) Adult Midgut  (545.1) Adult Hindgut  (945.7) Adult Malpighian Tubules  (1977.3) Adult Fat Body  (3827.3) Adult Salivary Gland no informative data Adult Heart  (2915.3) Adult VirginFemale Spermatheca  (4206.5) Adult InseminatedFemale Spermatheca  (2897.6) Adult Ovary    479.6 Adult Testis  (785.1) Adult Male Accessory Gland    276.1 Adult Carcass  (2830.2) Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    672.15 Larval Midgut    734.9 Larval Hindgut    664.4 Larval Malpighian Tubules  (1087.9) Larval Fat Body    440 Larval Salivary Gland no informative data Larval Trachea    216.25 Larval Carcass    762.4 Adult Head  (1514.1) Adult Eye    868.3 Adult Brain    456.2 Adult Thoracic-Abdominal Ganglion    557.7 Adult Crop  (2003.7) Adult Midgut    545.1 Adult Hindgut    945.7 Adult Malpighian Tubules  (1977.3) Adult Fat Body  (3827.3) Adult Salivary Gland no informative data Adult Heart  (2915.3) Adult VirginFemale Spermatheca  (4206.5) Adult InseminatedFemale Spermatheca  (2897.6) Adult Ovary    479.6 Adult Testis    785.1 Adult Male Accessory Gland    276.1 Adult Carcass  (2830.2) Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    672.15 Larval Midgut    734.9 Larval Hindgut    664.4 Larval Malpighian Tubules    1087.9 Larval Fat Body    440 Larval Salivary Gland no informative data Larval Trachea    216.25 Larval Carcass    762.4 Adult Head    1514.1 Adult Eye    868.3 Adult Brain    456.2 Adult Thoracic-Abdominal Ganglion    557.7 Adult Crop    2003.7 Adult Midgut    545.1 Adult Hindgut    945.7 Adult Malpighian Tubules    1977.3 Adult Fat Body    3827.3 Adult Salivary Gland no informative data Adult Heart    2915.3 Adult VirginFemale Spermatheca    4206.5 Adult InseminatedFemale Spermatheca    2897.6 Adult Ovary    479.6 Adult Testis    785.1 Adult Male Accessory Gland    276.1 Adult Carcass    2830.2 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0002719 expression level Tissue   Expression Level Larval Central Nervous System    672.15 Larval Midgut    734.9 Larval Hindgut    664.4 Larval Malpighian Tubules    1087.9 Larval Fat Body    440 Larval Salivary Gland no informative data Larval Trachea    216.25 Larval Carcass    762.4 Adult Head    1514.1 Adult Eye    868.3 Adult Brain    456.2 Adult Thoracic-Abdominal Ganglion    557.7 Adult Crop    2003.7 Adult Midgut    545.1 Adult Hindgut    945.7 Adult Malpighian Tubules    1977.3 Adult Fat Body    3827.3 Adult Salivary Gland no informative data Adult Heart    2915.3 Adult VirginFemale Spermatheca    4206.5 Adult InseminatedFemale Spermatheca    2897.6 Adult Ovary    479.6 Adult Testis    785.1 Adult Male Accessory Gland    276.1 Adult Carcass    2830.2 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  672.15 Larval Midgut  734.9 Larval Hindgut  664.4 Larval Malpighian Tubules  (1087.9) Larval Fat Body    440 Larval Salivary Gland no informative data Larval Trachea    216.25 Larval Carcass  762.4 Adult Head  (1514.1) Adult Eye  (868.3) Adult Brain    456.2 Adult Thoracic-Abdominal Ganglion  557.7 Adult Crop  (2003.7) Adult Midgut  545.1 Adult Hindgut  (945.7) Adult Malpighian Tubules  (1977.3) Adult Fat Body  (3827.3) Adult Salivary Gland no informative data Adult Heart  (2915.3) Adult VirginFemale Spermatheca  (4206.5) Adult InseminatedFemale Spermatheca  (2897.6) Adult Ovary    479.6 Adult Testis  785.1 Adult Male Accessory Gland    276.1 Adult Carcass  (2830.2) Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    672.15 Larval Midgut    734.9 Larval Hindgut    664.4 Larval Malpighian Tubules  1087.9 Larval Fat Body    440 Larval Salivary Gland no informative data Larval Trachea    216.25 Larval Carcass    762.4 Adult Head  1514.1 Adult Eye    868.3 Adult Brain    456.2 Adult Thoracic-Abdominal Ganglion    557.7 Adult Crop  (2003.7) Adult Midgut    545.1 Adult Hindgut    945.7 Adult Malpighian Tubules  (1977.3) Adult Fat Body  (3827.3) Adult Salivary Gland no informative data Adult Heart  (2915.3) Adult VirginFemale Spermatheca  (4206.5) Adult InseminatedFemale Spermatheca  (2897.6) Adult Ovary    479.6 Adult Testis    785.1 Adult Male Accessory Gland    276.1 Adult Carcass  (2830.2) Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    672.15 Larval Midgut    734.9 Larval Hindgut    664.4 Larval Malpighian Tubules    1087.9 Larval Fat Body    440 Larval Salivary Gland no informative data Larval Trachea    216.25 Larval Carcass    762.4 Adult Head    1514.1 Adult Eye    868.3 Adult Brain    456.2 Adult Thoracic-Abdominal Ganglion    557.7 Adult Crop    2003.7 Adult Midgut    545.1 Adult Hindgut    945.7 Adult Malpighian Tubules    1977.3 Adult Fat Body    3827.3 Adult Salivary Gland no informative data Adult Heart    2915.3 Adult VirginFemale Spermatheca    4206.5 Adult InseminatedFemale Spermatheca    2897.6 Adult Ovary    479.6 Adult Testis    785.1 Adult Male Accessory Gland    276.1 Adult Carcass    2830.2 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland no informative data Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland no informative data Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    1514.1   NA  Eye    868.3   NA  Brain    456.2   672.15  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    557.7   NA  Crop    2003.7   734.9  Midgut    545.1   664.4  Hindgut    945.7   1087.9  Malpighian Tubules    1977.3   440  Fat Body    3827.3   no informative data  Salivary Gland    no informative data   NA  Heart    2915.3   216.25  Trachea    NA   NA  VirginFemale Spermatheca    4206.5   NA  InseminatedFemale Spermatheca    2897.6   NA  Ovary    479.6   NA  Testis    785.1   NA  Male Accessory Gland    276.1   762.4  Carcass    2830.2 modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_20
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0002719 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG10120-RB FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for Men Stages(s) 1-3 Stages(s) 4-6 Stages(s) 9-10 Stages(s) 11-12 Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable MenCC06325 MenT190-26 viable | poor MenEx9- MenEx15- Sterility Allele fertile | poor MenEx9- MenEx15- Other Phenotypes Allele electrophoretic variant | fast Men6 electrophoretic variant | intermediate Men4 electrophoretic variant | slow Men2 visible, with Scer\GAL4GMR.PF MenEP1250 Phenotype manifest in Allele eye, with Scer\GAL4GMR.PF MenEP1250 germline cell | male, with Scer\GAL4ptc-559.1 MenEP500
Classical Alleles ( 63 )
For All Classical Alleles Show
Allele of Men Class Mutagen Stocks Known lesion Men4 spontaneous natural population 3 -- Men6 spontaneous natural population 3 -- Men5-HA-1025 1 -- Men5-HA-1631 1 -- Men5-HA-2188 1 -- Men5-SZ-3237 1 -- Men5-SZ-3451 1 -- MenBG02790 P-element activity 1 -- Menc01372 piggyBac transposase 1 -- MenCB-0569-3 1 -- MenCB-6459-3 1 -- Mend01330 P-element activity 1 -- MenDG05708 P-element activity 1 -- MenEY01756b P-element activity 1 -- MenEY06399 P-element activity 1 -- Menf01150 1 -- MenMI01213 1 -- MennNC1 loss of function allele spontaneous natural population 1 -- MennNC2 loss of function allele spontaneous natural population 1 -- MennNC3 loss of function allele spontaneous natural population 1 -- MenNP0587 1 -- MenNP0830 1 -- MenNP1333 1 -- MenNP1367 1 -- MenNP3447 1 -- MenNP4440 1 -- MenNP5383 1 -- MenNP6538 1 -- MenNP6610 1 -- MenNP7439 1 -- MenUM-8082-3 1 -- Men113A natural population 0 Yes Men113G natural population 0 Yes Men2 spontaneous natural population 0 -- Men338G natural population 0 -- MenCC06325 P-element activity 0 Yes MenCPTI002759 piggyBac transposase 0 Yes MenCPTI004074 piggyBac transposase 0 Yes Mend07397 P-element activity 0 -- Mend10913 P-element activity 0 -- MenEP1250 P-element activity 0 Yes MenEP3092 P-element activity 0 Yes MenEP309 P-element activity 0 Yes MenEP3376 P-element activity 0 -- MenEP377 P-element activity 0 Yes MenEP500 P-element activity 0 -- MenEP507 P-element activity 0 -- MenEP517 P-element activity 0 Yes MenEP675 P-element activity 0 Yes MenEx15- Delta2-3 0 Yes MenEx3+ Delta2-3 0 Yes MenEx5+ Delta2-3 0 Yes MenEx9- Delta2-3 0 Yes MenGCC natural population 0 -- MenGS9039 0 Yes MenKG00342 0 -- MennGB1 loss of function allele spontaneous natural population 0 -- MennNC311 spontaneous 0 -- MennNC4 loss of function allele spontaneous natural population 0 -- MennNC506 spontaneous 0 -- MennNC5 loss of function allele spontaneous natural population 0 -- MennNC6 loss of function allele spontaneous natural population 0 -- MenT190-26 P-element activity 0 Yes
Alleles Carried on Transgenic Constructs ( 4 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Men Class Mutagen Stocks Known lesion MenKK107809 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes MendsRNA.Scer\UAS in vitro construct - regulatory fusion in vitro construct - RNAi 0 Yes MenGD16565 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes MenGD6873 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3R)kar3l (Leonhardt et al., 1993) Df(3R)kar-lG27 (Leonhardt et al., 1993) Df(3R)kar-Sz31 (Leonhardt et al., 1993) Df(3R)ry615 (Leonhardt et al., 1993)
Not disrupted in	Df(3R)kar-Sz13 (Leonhardt et al., 1993) Df(3R)kar-Sz21 (Leonhardt et al., 1993) Df(3R)kar-Sz29 (Leonhardt et al., 1993) Df(3R)ry27 (Leonhardt et al., 1993) Df(3R)ry75 (Leonhardt et al., 1993)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD6873} NA P{GD16565} NA P{KK107809} NA P{UAS-Men.dsRNA} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EP}MenEP309 NA P{EP}MenEP377 NA P{EP}MenEP500 NA P{EP}MenEP507 NA P{EP}MenEP517 NA P{EP}MenEP675 NA P{EP}MenEP1250 NA P{EP}MenEP3092 NA P{EP}MenEP3376 NA P{EPgy2}MenEY01756b NA P{EPgy2}MenEY06399 NA P{GSV6}MenGS9039 NA P{XP}Mend01330 NA P{XP}Mend07397 NA P{XP}Mend10913 NA miscellaneous insertions Mi{MIC}MenMI01213 NA P{GT1}MenBG02790 NA P{PTT-GC}MenCC06325 NA P{RS3}MenCB-0569-3 NA P{RS3}MenCB-6459-3 NA P{RS3}MenUM-8082-3 NA P{RS5}Men5-HA-1025 NA P{RS5}Men5-HA-1631 NA P{RS5}Men5-HA-2188 NA P{RS5}Men5-SZ-3237 NA P{RS5}Men5-SZ-3451 NA P{SUPor-P}MenKG00342 NA P{T1}MenT190-26 NA P{wHy}MenDG05708 NA PBac{806.LOX-SVS-2}MenCPTI004074 NA PBac{CPTI-un}MenCPTI002759 NA PBac{PB}Menc01372 NA PBac{WH}Menf01150 NA insertion of enhancer trap binary system P{GawB}MenNP0587 No P{GawB}MenNP0830 No P{GawB}MenNP1333 No P{GawB}MenNP1367 No P{GawB}MenNP3447 No P{GawB}MenNP4440 No P{GawB}MenNP5383 No P{GawB}MenNP6538 No P{GawB}MenNP6610 No P{GawB}MenNP7439 No
Gene Ontology: Function, Process & Cellular Component ( 7 unique terms )
Terms Based on Experimental Evidence ( 2 terms )
Molecular Function
CV term Evidence References
malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) activity inferred from direct assay (Geer et al., 1980)
Biological Process
CV term Evidence References
regulation of cell death inferred from mutant phenotype (Yang et al., 2010)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 6 terms )
Molecular Function
CV term Evidence References
malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) activity inferred from sequence or structural similarity with UniProtKB:P28227 (FlyBase, 1992-) malate dehydrogenase (oxaloacetate-decarboxylating) activity inferred from electronic annotation with InterPro:IPR001891 (FlyBase Curators et al., 2004-) metal ion binding inferred from electronic annotation with InterPro:IPR015884 (FlyBase Curators et al., 2004-) NAD binding inferred from electronic annotation with InterPro:IPR012302 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
malate metabolic process inferred from electronic annotation with InterPro:IPR001891 (FlyBase Curators et al., 2004-) oxidation-reduction process inferred from electronic annotation with InterPro:IPR001891, InterPro:IPR012301, InterPro:IPR012302, InterPro:IPR015884 (FlyBase Curators et al., 2004-)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Men allele Gene References unspecified H (Mueller et al., 2005) pbl (Gregory et al., 2007)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 70650 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0002719 DroID - A comprehensive database of gene and protein interactions. • FBgn0002719 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 47173
Orthologs
Genome-wide drosophilid orthologs	Dana\GF18099 Dere\GG17100 Dgri\GH19210 Dmoj\GI24244 Dpse\GA10087 Dsec\GM25984 Dsim\Men Dvir\GJ10817 Dwil\GK10867 Dyak\GE24490
Curated drosophilid orthologs	Daff\Men   Dafs\Men (Berrigan and Hoang, 1999) Dame\Men   Dbor\Men   Dbus\Men   Dbuz\Men   Dezo\Men   Dfla\Men   Dkan\Men   Dlac\Men   Dlit\Men   Dlum\Men   Dmau\Men   Dmon\Men   Dnov\Men   Dpha\Men   Dpse\GA10087   Dsec\Men   Dsim\Men   Dsub\Men (Loukas and Krimbas, 1974) Dtei\Men   Dtex\Men   Dvir\Men   Dyak\Men
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AAEL005790-PA Aedes aegypti (mosquito) AGAP000184-PA Anopheles gambiae (mosquito) XP_395280.3 Apis mellifera (honey bee) XP_001943996.1 Acyrthosiphon pisum (pea aphid) BGIBMGA006419-PA Bombyx mori (silk worm) ENSBTAP00000007465 Bos taurus (bovine) CAL0004908 Candida albicans (a form of yeast) cn14103 Cryptococcus neoformans (yeast-like fungus) CPIJ012341 Culex pipens (mosquito) 126820 Chlamidomonas reinhardtii (green alga) 226972 Capitella spl (polychaete worm) DEHA2F12012g Debaryomyces Hansenii (yeast) ENSECAP00000022158 Equus caballus (horse) ENSGALP00000025506 Gallus gallus (chicken) ENSP00000358719 Homo sapiens (human) ISCW011260-PA Ixodes scapularis (deer tick) ENSMODP00000022921 Monodelphis domestica (gray opossum) ENSMUSP00000034989 Mus musculus (house mouse) XP_001608277.1 Nasonia vitripennis (wasp) ENSOANP00000002031 Ornithorynchus anatinus (platypus) ENSORLP00000002640 Oryzias latipes (japanese killifish) LOC_Os01g54030.1 Oryza sativa (asian rice) LOC_Os05g09440.1 Oryza sativa (asian rice) 9237 Ostreococcus tauri (green alga) ENSPTRP00000031411 Pan troglodytes (chimpanzee) ENSRNOP00000013244 Rattus norvegicus (norway rat) 50795 Trichoplax adhaerens (metazoa) XP_969226.1 Tribolium castaneum (red flour beetle) 86485.m00602 Trichomonas vaginalis (parasitic flagellated protozoan) 88273.m00372 Trichomonas vaginalis (parasitic flagellated protozoan) 89154.m00274 Trichomonas vaginalis (parasitic flagellated protozoan) ENSXETP00000022903 Xenopus tropicalis (frog) ENSXETP00000049052 Xenopus tropicalis (frog) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB13772 NV10467 LH13502 ISCW011260 FBgn0241604 FBgn0212878 FBgn0198088 FBgn0020700 FBgn0180840 JGI_V11_304467 FBgn0070146 FBgn0160757 FBgn0146967 FBgn0126677 FBgn0109328 FBgn0095117 CPIJ012341 BGIBMGA006419 ACYPI004126 GB16951 AGAP000184 AAEL005790 GLEAN_05233
Stocks & Reagents
Stocks Listed in FlyBase ( 33 )
Bloomington	4025 MennNC3 Aldox-1nNC4/TM2 12824 w1118; P{GT1}MenBG02790
Harvard	- P{XP}Mend01330 - PBac{PB}Menc01372 - PBac{WH}Menf01150
Kyoto	101689 MennNC1 / TM6 101690 MennNC2 / TM6 113281 w*; P{GawB}MenNP3447 123240 w1118; P{RS3}MenCB-0569-3 124110 w1118; P{RS3}MenCB-6459-3 124328 w1118; P{RS3}MenUM-8082-3
VDRC	v104016 P{KK107809}VIE-260B
	More stocks available...
Genomic Clones ( 2 )
	BACR07J06 BACR09O13 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 154 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD27718
Other clones	DPiM_LD27718 GH13028
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT12180 AT20282 AT30570 GH01823 GH05262 GH08372 GH20580 GH20588 GH22005 GH23159 GH23172 GH23789 GH24730 HL01657 LP01319 LP02290 LP09407 LP09482 LP12392 RE02557 RE08592 RE16380 RE22288 RE25844 RE33450 RE34877 RE44001 RE49449 RE51349 RE53820 RE61672 RE67963 RE70649 RE73604 RH04648 RH06152 RH06993 RH07418 RH08406 RH08462 RH08505 RH10213 RH12087 RH21319 RH21704 RH22039 RH22310 RH23307 RH23637 RH27221 RH36277 RH46094 RH50442 RH50692 RH51126 RH54293 RH56437 RH57708 RH58993 RH62906 RH70204 RH70250 RH70256 SD02333 SD02896 SD03653 SD03796 SD07229 SD13080 SD14622 SD15140 SD16523 SD20472 SD21923 SD23009 SD23161 SD24840 SD25649 SD26902 SD27705 SD27732
Other clones	40128 58391 165231 214437.44 bs12h01 bs25b08 bs59f08 CK01.15A.F10 CK01.18A.H2 Dmel_dig_003767_0712_1854 Dmel_dig_007098_0711_2923 Dmel_dig_010779_0613_3746 Dmel_dig_020947_0689_3135 Dmel_neb_007523_0693_0461 Dmel_neb_010401_0894_2677 Dmel_neb_013955_0325_1524 Dmel_neb_014197_0343_1669 Dmel_neb_016030_0562_2294 EK153917 EK181339 EK232003 EK269711 EP01614 EP17906 ESG0116a.B21_A12 FGC006B09 FGM236B11 FGM318G02 RE01945 RM05242 RM05542 RM05942 RM06042 RM06142 RM06242 RM06342 RM06442 RM06542 RP01422 RP01582 RP001001289 RP001001758 RP001003410 RP001003542 RP001004650 RP001004842 RP001011274 RP001011964 RP001012392 RP001012865 RP001013125 RP001013219 RP001014563 RP001014921 RP001015099 RP001024622 RP001028387 RP001028571 RP001030794 RP001031003 RP001032375 RP001036918 RP001037726 RP001038525 RP001043425 RP001043892 RP001046772 RP001047557 RP001049465 RP001052922
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0002719 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 47173
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: Men anon-WO0118547.278 (FlyBase, 1996-) Source for merge of: Men CG10120 (Bayraktaroglu, 2000.8.7)
Additional comments	"Men" may correspond to "Scim32". Sequence analysis off ends of P{SUPor-P} in Scim insertion mutant places "Scim32" near/in "Men". (Dobie and Karpen, 2001.4.18) Source for merge of Men anon-WO0118547.278 was sequence comparison (date:051113). (FlyBase, 1996-)
Other Comments
	Area matching Drosophila EST AA567704. (Davies, 2001.3.15) The effects of a high sucrose diet on live weight, total protein, stored lipid and glycogen and crude activities of 12 enzymes involved in energy metabolism are quantified. The activities of many enzymes are reduced by the sucrose treatment. (Wang and Clark, 1995) Gramoxone has no mutagenic effect on the genetic background of Men. (el Abidin Salam et al., 1993) Presence of an extra dose of the region from 8D10-12 to 9A1-2 results in an approximately 25% increase in Men activity. (Williamson and Bentley, 1983) All null alleles tested survive and are fertile in combination with Df(3R)ry31, which lacks Men+. (Voelker et al., 1980) The NADPH/NADP ratio in null genotypes is one-seventh that of wild type. (Geer et al., 1979)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_080141 NM_169479 RefSeq (Proteins) • NP_524880 NP_731739 DDBJ / EMBL / GenBank • AA567704 AA948992 AAF43601 AAF43602 AAF43603 AAF54859 AAF54860 AAM49909 ABA19464 ABA19465 ABA19466 ABA19467 ABA19468 ABA19469 ABA19470 ABA19471 ABA19472 ABA19473 ABA19474 ABA19475 ABA19476 ABA19477 ABA19478 ABA19479 ABA19480 ABA19481 ABA19482 ABA19483 ABA19484 ABA19485 ABA19486 ABA19487 ABA19488 ABA19489 ABA19490 ABA19491 ABA19492 ABA19493 ABA19494 ABA19495 ABA19496 AF187998 AF187999 AF188000 AI402720 AI404174 AI945435 AI946400 AJ251545 AQ025061 AQ025071 AQ025097 AQ025100 AQ025102 AQ025207 AQ025272 AQ073955 AQ254603 AW942773 AX094154 AY118540 BH146183 CAB64262 CZ467108 CZ472165 CZ472166 CZ475708 CZ477499 DQ148977 DQ148978 DQ148979 DQ148980 DQ148981 DQ148982 DQ148983 DQ148984 DQ148985 DQ148986 DQ148987 DQ148988 DQ148989 DQ148990 DQ148991 DQ148992 DQ148993 DQ148994 DQ148995 DQ148996 DQ148997 DQ148998 DQ148999 DQ149000 DQ149001 DQ149002 DQ149003 DQ149004 DQ149005 DQ149006 DQ149007 DQ149008 DQ149009 Entrez Gene - A searchable database of RefSeq genes. • 47173 UniProtKB/TrEMBL • Q2QBM1 Q9NIW0 Q9NIW1 Q9NIW2 Q9U1J1 Q9VG31 Q9VG32
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Malic oxidoreductase (IPR001891) Malic enzyme, N-terminal (IPR012301) Malic enzyme, NAD-binding (IPR012302) Malic enzyme, conserved site (IPR015884) NAD(P)-binding domain (IPR016040)
Linkouts
	BioGRID - A database of protein and genetic interactions • 70650 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0002719 DroID - A comprehensive database of gene and protein interactions. • FBgn0002719 DRSC - Results from RNAi screens. • FBgn0002719 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0002719 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG10120-RB FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0002719 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 47173 InParanoid A subset of ortholog calls from InParanoid. • AAEL005790-PA Aedes aegypti (mosquito) AGAP000184-PA Anopheles gambiae (mosquito) XP_395280.3 Apis mellifera (honey bee) XP_001943996.1 Acyrthosiphon pisum (pea aphid) BGIBMGA006419-PA Bombyx mori (silk worm) ENSBTAP00000007465 Bos taurus (bovine) CAL0004908 Candida albicans (a form of yeast) cn14103 Cryptococcus neoformans (yeast-like fungus) CPIJ012341 Culex pipens (mosquito) 126820 Chlamidomonas reinhardtii (green alga) 226972 Capitella spl (polychaete worm) DEHA2F12012g Debaryomyces Hansenii (yeast) ENSECAP00000022158 Equus caballus (horse) ENSGALP00000025506 Gallus gallus (chicken) ENSP00000358719 Homo sapiens (human) ISCW011260-PA Ixodes scapularis (deer tick) ENSMODP00000022921 Monodelphis domestica (gray opossum) ENSMUSP00000034989 Mus musculus (house mouse) XP_001608277.1 Nasonia vitripennis (wasp) ENSOANP00000002031 Ornithorynchus anatinus (platypus) ENSORLP00000002640 Oryzias latipes (japanese killifish) LOC_Os01g54030.1 Oryza sativa (asian rice) LOC_Os05g09440.1 Oryza sativa (asian rice) 9237 Ostreococcus tauri (green alga) ENSPTRP00000031411 Pan troglodytes (chimpanzee) ENSRNOP00000013244 Rattus norvegicus (norway rat) 50795 Trichoplax adhaerens (metazoa) XP_969226.1 Tribolium castaneum (red flour beetle) 86485.m00602 Trichomonas vaginalis (parasitic flagellated protozoan) 88273.m00372 Trichomonas vaginalis (parasitic flagellated protozoan) 89154.m00274 Trichomonas vaginalis (parasitic flagellated protozoan) ENSXETP00000022903 Xenopus tropicalis (frog) ENSXETP00000049052 Xenopus tropicalis (frog) InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 47173 modMine - Data generated by the modENCODE project. • FBgn0002719 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB13772 NV10467 LH13502 ISCW011260 FBgn0241604 FBgn0212878 FBgn0198088 FBgn0020700 FBgn0180840 JGI_V11_304467 FBgn0070146 FBgn0160757 FBgn0146967 FBgn0126677 FBgn0109328 FBgn0095117 CPIJ012341 BGIBMGA006419 ACYPI004126 GB16951 AGAP000184 AAEL005790 GLEAN_05233
Synonyms & Secondary IDs ( 12 )
Reported As
Symbol Synonym	anon-WO0118547.278   CG10120 (Pile et al., 2003, Seegmiller et al., 2002, Dobie and Karpen, 2001.4.18, Gregory et al., 2007, Toledano-Katchalski et al., 2007, O'Keefe et al., 2011, Keleman et al., 2009.8.5, Rach et al., 2009, Lai et al., 2007, Cammarato et al., 2011) MDH (Farkas and Knopp, 1998) Mdh-NADP (Franklin and Rumball, 1971, ) ME (Montooth et al., 2003, Bondinas et al., 2002, Bondinas et al., 2001, Harshman et al., 1999, Clark and Fucito, 1998, Clark and Wang, 1997, Wang and Clark, 1995, Clark, 1994, Clark and Wang, 1994, Clark et al., 1995, Choudhary et al., 1992, Clark et al., 1990, Clark, 1990, Deckert-Cruz et al., 2004) Me (Zapata et al., 2002, Harshman and Schmid, 1998, Matthews and Munstermann, 1994, el Abidin Salam et al., 1993, ) mem (Seugnet et al., 2009) Men (Reeves and Posakony, 2005, Buszczak et al., 2007, Haynes et al., 2006, O'Keefe et al., 2011, Morozova et al., 2009, Morozova et al., 2007, Franciscovich et al., 2008, Wasbrough et al., 2010, Yang et al., 2010) men (Harvey et al., 2008, Rach et al., 2009)
Name Synonym	Malic enzyme (Harbison and Sehgal, 2008, Deckert-Cruz et al., 2004, Seugnet et al., 2009, Morozova et al., 2009, Morozova et al., 2007, Robinson et al., 2010, Barnes et al., 2010, Cammarato et al., 2011) malic enzyme (Montooth et al., 2003, O'Keefe et al., 2011, Morozova et al., 2006, Yang et al., 2010) NADP-malic enzyme (Wegulo and Williamson, 1994, Bowen and Williamson, 1994)
Secondary FlyBase IDs
	FBgn0038081 FBgn0062124
References ( 105 )
Generate a list of	All references relating to this gene ( 105 )
List References by type	Research paper  ( 75 ) Supplementary material  ( 7 ) Personal communication to FlyBase  ( 10 ) FlyBase analysis  ( 3 ) Patent  ( 1 ) DNA/RNA sequence record  ( 3 ) Computer file  ( 3 ) Automatic genome annotation  ( 2 ) Book  ( 1 )
Recent research papers ( 6 )
	Lum and Merritt, 2011, Genetics 189(3): 837--849 Nonclassical Regulation of Transcription: Interchromosomal Interactions at the Malic enzyme Locus of Drosophila melanogaster. [FBrf0216683] Morozova et al., 2011, Genetics 187(4): 1193--1205 Transcriptional networks for alcohol sensitivity in Drosophila melanogaster. [FBrf0214377] O'Keefe et al., 2011, Hum. Mol. Genet. 20(3): 497--509 Drosophila orthologue of WWOX, the chromosomal fragile site FRA16D tumour suppressor gene, functions in aerobic metabolism and regulates reactive oxygen species. [FBrf0212691] Barnes et al., 2010, BMC Biochem. 11: 26 Loss of the SIN3 transcriptional corepressor results in aberrant mitochondrial function. [FBrf0211360] Robinson et al., 2010, Mech. Ageing Dev. 131(9): 584--590 Insights into aging through measurements of the Drosophila proteome as a function of temperature. [FBrf0211959] Yang et al., 2010, EMBO J. 29(18): 3196--3207 Metabolic regulation of Drosophila apoptosis through inhibitory phosphorylation of Dronc. [FBrf0211843] Show all research papers ...