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Gene Dmel\Met

General Information
Symbol	Dmel\Met	Species	D. melanogaster
Name	Methoprene-tolerant	Annotation symbol	CG1705
Feature type	protein_coding_gene	FlyBase ID	FBgn0002723
Gene Model Status	Current	Stock availability	16 publicly available
Also Known As	Rst(1)JH, DmMet
Genomic Location
Chromosome (arm)	X	Recombination map	1-35.4
Cytogenetic map	10D1-10D1	Sequence location	X:11,510,159..11,513,981 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Methoprene-tolerant is referred to in FlyBase by the symbol Dmel\Met (CG1705, FBgn0002723). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: transcription factor binding; protein heterodimerization activity; juvenile hormone binding; protein homodimerization activity. There is experimental evidence that it is involved in the biological process: positive regulation of transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter by nuclear hormone receptor; regulation of developmental process. 23 alleles are reported. The phenotypes of these alleles are annotated with: female germline cyst; organ system; gamete; organ system subdivision; lobula complex; adult segment; stage S9 oocyte; multi-cell-component structure; sensillum; region of integument. It has one annotated transcript and one annotated polypeptide. Protein features are: Helix-loop-helix DNA-binding; PAC motif; PAS. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderate expression to a trough of low expression. Peak expression observed at stages throughout embryogenesis, at stages throughout the larval period, at stages throughout the pupal period, in stages of adults of both sexes. Summary of FlyAtlas Anatomical Expression Data: Little or no expression detected in any larval or adult organs/tissues. Comments on Affy2 ProbeSet: ProbeSet 1630521_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of Met. Gene sequence location is X:11510159..11513981.
External Summaries
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	Rst(1)JH: Resistance (1) Juvenile Hormone Semidominant mutation conferring 50-100 fold increase in resistance to juvenile hormone III or its analogue methoprene over that of wild type. Also resistant to methoprene-induced tumors and abnormalities in adult cuticle; action of Rst(1)JH autonomous in gynandromorphs. Authors speculate that gene may affect juveline-hormone receptor.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2011_10	References Greb-Markiewicz et al., 2011, Molec. Cell. Endocrinol. 345(1-2): 16--26
FB2012_01	References Li et al., 2011, Proc. Natl. Acad. Sci. U.S.A. 108(2): 638--643 Dubrovsky et al., 2011, J. Biol. Chem. 286(38): 33689--33700 Sequence features BKN25522 HFA20284 Controlled Vocabulary Terms positive regulation of transcription from RNA polymerase II promoter transcription factor binding regulation of transcription from RNA polymerase II promoter by nuclear hormone receptor
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 10D1-10D1   Limits computationally determined from genome sequence between P{EP}CG32666EP1452 and P{EP}Ptp10DEP1172
Experimentally Determined Cytological Location
Cytogenetic map Notes References 10C5-10C5     (Wilson, 1997.11.17) 10C-10C   (determined by in situ hybridisation)   (Turner and Wilson, 1995) 10C5-10C5   (determined by in situ hybridisation)   (Turner et al., 1991)
Experimentally Determined Recombination Data
Location	1-35.4 (Wilson and Fabian, 1986)
Left of (cM)	m f (Wilson and Fabian, 1986)
Right of (cM)	v (Wilson and Fabian, 1986)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Met for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Met-RA FBtr0073521  NM_078571   3754   716
Additional Transcript Data & Comments
Reported size (kB)	5.5, 3.3 (northern blot) (Ashok et al., 1998)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Met-PA   FBpp0073368   78.7   716   6.74     NP_511126   AAF48071
Additional Polypeptide Data & Comments
Reported size (kDa)	716 (aa); 79 (kD predicted) (Ashok et al., 1998) 85 (kD) (Shemshedini and Wilson, 1993)
Comments	Met protein is specifically competed by juvenile hormone III. (Shemshedini and Wilson, 1993)
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • PAS (IPR000014) Helix-loop-helix DNA-binding (IPR011598) PAC motif (IPR001610)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA698003   AE014298 AAF48071   AF034859 AAC14350   BG638420   BP541439   BP554867   BT024194   BT099669   CO155307   CO188278   CO304506   CO316989   CO328102   EC202817   EC212326   EC238566   EC255280   EC267473   EL879268
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	O44118 Q9VYW2
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
northern blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage     (Ashok et al., 1998) embryonic stage -- adult stage     (Ashok et al., 1998) adult stage | female     (Ashok et al., 1998)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term	cytoplasm (Shemshedini and Wilson, 1993)
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0002723    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0002723 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderate expression to a trough of low expression. Peak expression observed at stages throughout embryogenesis, at stages throughout the larval period, at stages throughout the pupal period, in stages of adults of both sexes. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0002723 expression level Developmental Stage   Expression Level embryo 00-02hr    492 embryo 02-04hr    417 embryo 04-06hr    401 embryo 06-08hr    608 embryo 08-10hr    611 embryo 10-12hr    715 embryo 12-14hr    436 embryo 14-16hr    779 embryo 16-18hr    962 embryo 18-20hr    1201 embryo 20-22hr    1044 embryo 22-24hr    922 larva L1    731 larva L2    400 larva L3 12hr old    422 larva L3 puffstage 1-2    234 larva L3 puffstage 3-6    463 larva L3 puffstage 7-9    438 white prepupae new    407 white prepupae 12hr    580 white prepupae 24hr    579 pupae 2d postWPP    482 pupae 3d postWPP    403 pupae 4d postWPP    564 adult male 01day    532 adult male 05day    584 adult male 30day    621 adult female 01day    774 adult female 05day    516 adult female 30day    585 Expression Level Scale  None   Extremely low   Very low   Low   Moderate  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (492) embryo 02-04hr  (417) embryo 04-06hr  (401) embryo 06-08hr  (608) embryo 08-10hr  (611) embryo 10-12hr  (715) embryo 12-14hr  (436) embryo 14-16hr  (779) embryo 16-18hr  (962) embryo 18-20hr  (1201) embryo 20-22hr  (1044) embryo 22-24hr  (922) larva L1  (731) larva L2  (400) larva L3 12hr old  (422) larva L3 puffstage 1-2  (234) larva L3 puffstage 3-6  (463) larva L3 puffstage 7-9  (438) white prepupae new  (407) white prepupae 12hr  (580) white prepupae 24hr  (579) pupae 2d postWPP  (482) pupae 3d postWPP  (403) pupae 4d postWPP  (564) adult male 01day  (532) adult male 05day  (584) adult male 30day  (621) adult female 01day  (774) adult female 05day  (516) adult female 30day  (585) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    492 embryo 02-04hr    417 embryo 04-06hr    401 embryo 06-08hr    608 embryo 08-10hr    611 embryo 10-12hr    715 embryo 12-14hr    436 embryo 14-16hr    779 embryo 16-18hr    962 embryo 18-20hr    1201 embryo 20-22hr    1044 embryo 22-24hr    922 larva L1    731 larva L2    400 larva L3 12hr old    422 larva L3 puffstage 1-2    234 larva L3 puffstage 3-6    463 larva L3 puffstage 7-9    438 white prepupae new    407 white prepupae 12hr    580 white prepupae 24hr    579 pupae 2d postWPP    482 pupae 3d postWPP    403 pupae 4d postWPP    564 adult male 01day    532 adult male 05day    584 adult male 30day    621 adult female 01day    774 adult female 05day    516 adult female 30day    585 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    492 embryo 02-04hr    417 embryo 04-06hr    401 embryo 06-08hr    608 embryo 08-10hr    611 embryo 10-12hr    715 embryo 12-14hr    436 embryo 14-16hr    779 embryo 16-18hr    962 embryo 18-20hr    1201 embryo 20-22hr    1044 embryo 22-24hr    922 larva L1    731 larva L2    400 larva L3 12hr old    422 larva L3 puffstage 1-2    234 larva L3 puffstage 3-6    463 larva L3 puffstage 7-9    438 white prepupae new    407 white prepupae 12hr    580 white prepupae 24hr    579 pupae 2d postWPP    482 pupae 3d postWPP    403 pupae 4d postWPP    564 adult male 01day    532 adult male 05day    584 adult male 30day    621 adult female 01day    774 adult female 05day    516 adult female 30day    585 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    492 embryo 02-04hr    417 embryo 04-06hr    401 embryo 06-08hr    608 embryo 08-10hr    611 embryo 10-12hr    715 embryo 12-14hr    436 embryo 14-16hr    779 embryo 16-18hr    962 embryo 18-20hr    1201 embryo 20-22hr    1044 embryo 22-24hr    922 larva L1    731 larva L2    400 larva L3 12hr old    422 larva L3 puffstage 1-2    234 larva L3 puffstage 3-6    463 larva L3 puffstage 7-9    438 white prepupae new    407 white prepupae 12hr    580 white prepupae 24hr    579 pupae 2d postWPP    482 pupae 3d postWPP    403 pupae 4d postWPP    564 adult male 01day    532 adult male 05day    584 adult male 30day    621 adult female 01day    774 adult female 05day    516 adult female 30day    585 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0002723 expression level Developmental Stage   Expression Level embryo 00-02hr    492 embryo 02-04hr    417 embryo 04-06hr    401 embryo 06-08hr    608 embryo 08-10hr    611 embryo 10-12hr    715 embryo 12-14hr    436 embryo 14-16hr    779 embryo 16-18hr    962 embryo 18-20hr    1201 embryo 20-22hr    1044 embryo 22-24hr    922 larva L1    731 larva L2    400 larva L3 12hr old    422 larva L3 puffstage 1-2    234 larva L3 puffstage 3-6    463 larva L3 puffstage 7-9    438 white prepupae new    407 white prepupae 12hr    580 white prepupae 24hr    579 pupae 2d postWPP    482 pupae 3d postWPP    403 pupae 4d postWPP    564 adult male 01day    532 adult male 05day    584 adult male 30day    621 adult female 01day    774 adult female 05day    516 adult female 30day    585 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (492) embryo 02-04hr  (417) embryo 04-06hr  (401) embryo 06-08hr  (608) embryo 08-10hr  (611) embryo 10-12hr  (715) embryo 12-14hr  (436) embryo 14-16hr  (779) embryo 16-18hr  (962) embryo 18-20hr  (1201) embryo 20-22hr  (1044) embryo 22-24hr  (922) larva L1  (731) larva L2  (400) larva L3 12hr old  (422) larva L3 puffstage 1-2  (234) larva L3 puffstage 3-6  (463) larva L3 puffstage 7-9  (438) white prepupae new  (407) white prepupae 12hr  (580) white prepupae 24hr  (579) pupae 2d postWPP  (482) pupae 3d postWPP  (403) pupae 4d postWPP  (564) adult male 01day  (532) adult male 05day  (584) adult male 30day  (621) adult female 01day  (774) adult female 05day  (516) adult female 30day  (585) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    492 embryo 02-04hr    417 embryo 04-06hr    401 embryo 06-08hr    608 embryo 08-10hr    611 embryo 10-12hr    715 embryo 12-14hr    436 embryo 14-16hr    779 embryo 16-18hr    962 embryo 18-20hr    1201 embryo 20-22hr    1044 embryo 22-24hr    922 larva L1    731 larva L2    400 larva L3 12hr old    422 larva L3 puffstage 1-2    234 larva L3 puffstage 3-6    463 larva L3 puffstage 7-9    438 white prepupae new    407 white prepupae 12hr    580 white prepupae 24hr    579 pupae 2d postWPP    482 pupae 3d postWPP    403 pupae 4d postWPP    564 adult male 01day    532 adult male 05day    584 adult male 30day    621 adult female 01day    774 adult female 05day    516 adult female 30day    585 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    492 embryo 02-04hr    417 embryo 04-06hr    401 embryo 06-08hr    608 embryo 08-10hr    611 embryo 10-12hr    715 embryo 12-14hr    436 embryo 14-16hr    779 embryo 16-18hr    962 embryo 18-20hr    1201 embryo 20-22hr    1044 embryo 22-24hr    922 larva L1    731 larva L2    400 larva L3 12hr old    422 larva L3 puffstage 1-2    234 larva L3 puffstage 3-6    463 larva L3 puffstage 7-9    438 white prepupae new    407 white prepupae 12hr    580 white prepupae 24hr    579 pupae 2d postWPP    482 pupae 3d postWPP    403 pupae 4d postWPP    564 adult male 01day    532 adult male 05day    584 adult male 30day    621 adult female 01day    774 adult female 05day    516 adult female 30day    585 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    492 embryo 02-04hr    417 embryo 04-06hr    401 embryo 06-08hr    608 embryo 08-10hr    611 embryo 10-12hr    715 embryo 12-14hr    436 embryo 14-16hr    779 embryo 16-18hr    962 embryo 18-20hr    1201 embryo 20-22hr    1044 embryo 22-24hr    922 larva L1    731 larva L2    400 larva L3 12hr old    422 larva L3 puffstage 1-2    234 larva L3 puffstage 3-6    463 larva L3 puffstage 7-9    438 white prepupae new    407 white prepupae 12hr    580 white prepupae 24hr    579 pupae 2d postWPP    482 pupae 3d postWPP    403 pupae 4d postWPP    564 adult male 01day    532 adult male 05day    584 adult male 30day    621 adult female 01day    774 adult female 05day    516 adult female 30day    585 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0002723    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0002723 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Little or no expression detected in any larval or adult organs/tissues. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0002723 expression level Tissue   Expression Level Larval Central Nervous System    15.225 Larval Midgut    4.3 Larval Hindgut    6.5 Larval Malpighian Tubules    8.7 Larval Fat Body    7.2 Larval Salivary Gland no informative data Larval Trachea no informative data Larval Carcass no informative data Adult Head    7.9 Adult Eye    16.975 Adult Brain    31.9 Adult Thoracic-Abdominal Ganglion    31.1 Adult Crop no informative data Adult Midgut    5.6 Adult Hindgut no informative data Adult Malpighian Tubules    5.2 Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart no informative data Adult VirginFemale Spermatheca    5.4 Adult InseminatedFemale Spermatheca    8.4 Adult Ovary no informative data Adult Testis    5.6 Adult Male Accessory Gland    8.9 Adult Carcass no informative data Expression Level Scale  None   Low  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    15.225 Larval Midgut    4.3 Larval Hindgut    6.5 Larval Malpighian Tubules    8.7 Larval Fat Body    7.2 Larval Salivary Gland no informative data Larval Trachea no informative data Larval Carcass no informative data Adult Head    7.9 Adult Eye    16.975 Adult Brain    31.9 Adult Thoracic-Abdominal Ganglion    31.1 Adult Crop no informative data Adult Midgut    5.6 Adult Hindgut no informative data Adult Malpighian Tubules    5.2 Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart no informative data Adult VirginFemale Spermatheca    5.4 Adult InseminatedFemale Spermatheca    8.4 Adult Ovary no informative data Adult Testis    5.6 Adult Male Accessory Gland    8.9 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    15.225 Larval Midgut    4.3 Larval Hindgut    6.5 Larval Malpighian Tubules    8.7 Larval Fat Body    7.2 Larval Salivary Gland no informative data Larval Trachea no informative data Larval Carcass no informative data Adult Head    7.9 Adult Eye    16.975 Adult Brain    31.9 Adult Thoracic-Abdominal Ganglion    31.1 Adult Crop no informative data Adult Midgut    5.6 Adult Hindgut no informative data Adult Malpighian Tubules    5.2 Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart no informative data Adult VirginFemale Spermatheca    5.4 Adult InseminatedFemale Spermatheca    8.4 Adult Ovary no informative data Adult Testis    5.6 Adult Male Accessory Gland    8.9 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    15.225 Larval Midgut    4.3 Larval Hindgut    6.5 Larval Malpighian Tubules    8.7 Larval Fat Body    7.2 Larval Salivary Gland no informative data Larval Trachea no informative data Larval Carcass no informative data Adult Head    7.9 Adult Eye    16.975 Adult Brain    31.9 Adult Thoracic-Abdominal Ganglion    31.1 Adult Crop no informative data Adult Midgut    5.6 Adult Hindgut no informative data Adult Malpighian Tubules    5.2 Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart no informative data Adult VirginFemale Spermatheca    5.4 Adult InseminatedFemale Spermatheca    8.4 Adult Ovary no informative data Adult Testis    5.6 Adult Male Accessory Gland    8.9 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0002723 expression level Tissue   Expression Level Larval Central Nervous System    15.225 Larval Midgut    4.3 Larval Hindgut    6.5 Larval Malpighian Tubules    8.7 Larval Fat Body    7.2 Larval Salivary Gland no informative data Larval Trachea no informative data Larval Carcass no informative data Adult Head    7.9 Adult Eye    16.975 Adult Brain    31.9 Adult Thoracic-Abdominal Ganglion    31.1 Adult Crop no informative data Adult Midgut    5.6 Adult Hindgut no informative data Adult Malpighian Tubules    5.2 Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart no informative data Adult VirginFemale Spermatheca    5.4 Adult InseminatedFemale Spermatheca    8.4 Adult Ovary no informative data Adult Testis    5.6 Adult Male Accessory Gland    8.9 Adult Carcass no informative data Expression Level Scale  None   Low  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    15.225 Larval Midgut    4.3 Larval Hindgut    6.5 Larval Malpighian Tubules    8.7 Larval Fat Body    7.2 Larval Salivary Gland no informative data Larval Trachea no informative data Larval Carcass no informative data Adult Head    7.9 Adult Eye    16.975 Adult Brain    31.9 Adult Thoracic-Abdominal Ganglion    31.1 Adult Crop no informative data Adult Midgut    5.6 Adult Hindgut no informative data Adult Malpighian Tubules    5.2 Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart no informative data Adult VirginFemale Spermatheca    5.4 Adult InseminatedFemale Spermatheca    8.4 Adult Ovary no informative data Adult Testis    5.6 Adult Male Accessory Gland    8.9 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    15.225 Larval Midgut    4.3 Larval Hindgut    6.5 Larval Malpighian Tubules    8.7 Larval Fat Body    7.2 Larval Salivary Gland no informative data Larval Trachea no informative data Larval Carcass no informative data Adult Head    7.9 Adult Eye    16.975 Adult Brain    31.9 Adult Thoracic-Abdominal Ganglion    31.1 Adult Crop no informative data Adult Midgut    5.6 Adult Hindgut no informative data Adult Malpighian Tubules    5.2 Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart no informative data Adult VirginFemale Spermatheca    5.4 Adult InseminatedFemale Spermatheca    8.4 Adult Ovary no informative data Adult Testis    5.6 Adult Male Accessory Gland    8.9 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    15.225 Larval Midgut    4.3 Larval Hindgut    6.5 Larval Malpighian Tubules    8.7 Larval Fat Body    7.2 Larval Salivary Gland no informative data Larval Trachea no informative data Larval Carcass no informative data Adult Head    7.9 Adult Eye    16.975 Adult Brain    31.9 Adult Thoracic-Abdominal Ganglion    31.1 Adult Crop no informative data Adult Midgut    5.6 Adult Hindgut no informative data Adult Malpighian Tubules    5.2 Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart no informative data Adult VirginFemale Spermatheca    5.4 Adult InseminatedFemale Spermatheca    8.4 Adult Ovary no informative data Adult Testis    5.6 Adult Male Accessory Gland    8.9 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland no informative data Larval Trachea no informative data Larval Carcass no informative data Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop no informative data Adult Midgut     Adult Hindgut no informative data Adult Malpighian Tubules     Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart no informative data Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary no informative data Adult Testis     Adult Male Accessory Gland     Adult Carcass no informative data FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    7.9   NA  Eye    16.975   NA  Brain    31.9   15.225  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    31.1   NA  Crop    no informative data   4.3  Midgut    5.6   6.5  Hindgut    no informative data   8.7  Malpighian Tubules    5.2   7.2  Fat Body    no informative data   no informative data  Salivary Gland    no informative data   NA  Heart    no informative data   no informative data  Trachea    NA   NA  VirginFemale Spermatheca    5.4   NA  InseminatedFemale Spermatheca    8.4   NA  Ovary    no informative data   NA  Testis    5.6   NA  Male Accessory Gland    8.9   no informative data  Carcass    no informative data modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_09
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0002723 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG1705-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | larval stage, with Scer\GAL4Act.PU MetScer\UAS.cBa lethal | larval stage, with Scer\GAL4tub.PU MetScer\UAS.cBa lethal | partially | pupal stage Met27 lethal | recessive Met153 semi-lethal | larval stage, with Scer\GAL4[-]: MetScer\UAS.cBa semi-lethal | larval stage, with Scer\GAL4Act.PU MetScer\UAS.cBa semi-lethal | pupal stage, with Scer\GAL4pnr-MD237 MetGD4384 viable Met3 Met27 Met141 Sterility Allele female fertile | reduced Met27 Other Phenotypes Allele chemical resistant Met1 Met2 Met3 Met11 Met27 Met128 MetA3 MetD29 MetK17 MetN6 chemical resistant | dominant Met1 MetD29 MetK17 chemical resistant | semidominant Met1 chemical sensitive Met3 visible Met+tSt-H.hs visible, with Scer\GAL4[-]: MetScer\UAS.cBa wild-type Met+tSt-H.hs Meths.PA Phenotype manifest in Allele abdominal sternite bristle Met+tSt-H.hs abdominal sternite bristle, with Scer\GAL4[-]: MetScer\UAS.cBa embryonic/larval fat body | wandering third instar larval stage, with MetScer\UAS.cBa, Scer\GAL4Adh.PF MetW3 embryonic/larval fat body | wandering third instar larval stage, with MetW3, Scer\GAL4Adh.PF MetScer\UAS.cBa lobula Met27 lobula plate Met27 male genitalia Met+tSt-H.hs male genitalia, with Scer\GAL4[-]: MetScer\UAS.cBa optic lobe | prepupal stage Met27 ovary Met27 photoreceptor cell R7 | prepupal stage Met27 photoreceptor cell R8 | prepupal stage Met27 stage S8 oocyte Met27 stage S9 oocyte Met27 stage S10 oocyte Met27 stage S11 oocyte Met27 stage S12 oocyte Met27 stage S13 oocyte Met27 stage S14 oocyte Met27
Classical Alleles ( 15 )
For All Classical Alleles Show
Allele of Met Class Mutagen Stocks Known lesion Met1 amorphic allele - genetic evidence ethyl methanesulfonate 2 -- MetK17 amorphic allele - genetic evidence P-element activity 2 Yes Met3 hypomorphic allele - genetic evidence ethyl methanesulfonate 2 -- MetN6 X ray gamma ray 2 -- MetD29 amorphic allele - genetic evidence X ray gamma ray 1 -- MetA3 P-element activity 1 Yes Met11 X ray 0 -- Met128 X ray 0 -- Met141 P-element activity 0 Yes Met153 P-element activity 0 Yes Met253 0 -- Met27 loss of function allele X ray 60Co gamma ray 0 Yes Met2 ethyl methanesulfonate 0 -- MetE1 0 -- MetW3 0 --
Alleles Carried on Transgenic Constructs ( 8 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Met Class Mutagen Stocks Known lesion MetGD4384 in vitro construct - RNAi in vitro construct - regulatory fusion 4 Yes MetJF02103 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes MetKK104562 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Met+tSt-H.hs in vitro construct - regulatory fusion 0 Yes Meths.PA in vitro construct - regulatory fusion 0 Yes MetK-H.hs in vitro construct - regulatory fusion 0 Yes MetScer\UAS.cBa in vitro construct - regulatory fusion 0 Yes MetSt-X.hs in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(1)HA85 (Wilson and Fabian, 1986) Df(1)m13 (Shemshedini and Wilson, 1990) Df(1)m259-4 (Ashok et al., 1998, Wilson and Fabian, 1986, Minkoff and Wilson, 1992) Df(1)N71 (Wilson and Fabian, 1986, Shemshedini and Wilson, 1990, Minkoff and Wilson, 1992)
Not disrupted in	Df(1)JA26 (Wilson and Fabian, 1986) Df(1)KA6 (Wilson and Fabian, 1986) Df(1)KA10 (Wilson and Fabian, 1986) Df(1)N105 (Wilson and Fabian, 1986) Df(1)RA47 (Wilson and Fabian, 1986)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD4384} NA P{KK104562} NA P{TRiP.JF02103} NA P{UAS-Met.B} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions P{?'}Met141 NA P{}MetA3 NA P{}MetK17 NA
Gene Ontology: Function, Process & Cellular Component ( 12 unique terms )
Terms Based on Experimental Evidence ( 8 terms )
Molecular Function
CV term Evidence References
juvenile hormone binding inferred from direct assay (Miura et al., 2005) protein heterodimerization activity inferred from direct assay (Bitra and Palli, 2009) inferred from physical interaction with ftz-f1 (Dubrovsky et al., 2011) protein homodimerization activity inferred from direct assay (Bitra and Palli, 2009) transcription factor binding inferred from physical interaction with ftz-f1 (Dubrovsky et al., 2011)
Biological Process
CV term Evidence References
positive regulation of transcription from RNA polymerase II promoter inferred from direct assay (Miura et al., 2005) inferred from mutant phenotype (Dubrovsky et al., 2011) regulation of developmental process inferred from mutant phenotype (Flatt and Kawecki, 2004) regulation of transcription from RNA polymerase II promoter by nuclear hormone receptor inferred from direct assay (Miura et al., 2005)
Cellular Component
CV term Evidence References
nucleus inferred from direct assay (Miura et al., 2005)
Terms Based on Predictions or Assertions ( 6 terms )
Molecular Function
CV term Evidence References
juvenile hormone binding non-traceable author statement (FlyBase, 1992-) traceable author statement (Wheeler and Nijhout, 2003) sequence-specific DNA binding transcription factor activity inferred from sequence or structural similarity with EMBL:AB012600 (FlyBase, 1992-) signal transducer activity inferred from electronic annotation with InterPro:IPR000014 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
regulation of transcription, DNA-dependent traceable author statement (Wheeler and Nijhout, 2003) signal transduction inferred from electronic annotation with InterPro:IPR000014, InterPro:IPR001610 (FlyBase Curators et al., 2004-)
Cellular Component
CV term Evidence References
nucleus inferred from sequence or structural similarity with EMBL:AB012600 (FlyBase, 1992-) traceable author statement (Wheeler and Nijhout, 2003)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Met allele Gene References Rst(1)JHK17 Fas2 (Adam et al., 2003) unspecified br (Wilson et al., 2006)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 58523 DroID - A comprehensive database of gene and protein interactions. • FBgn0002723 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 32114
Orthologs
Genome-wide drosophilid orthologs	Dana\GF19442 Dere\GG18435 Dgri\GH24193 Dmoj\GI14831 Dper\GL13106 Dpse\GA14297 Dsec\GM13098 Dsim\GD17037 Dvir\Met Dwil\GK10105 Dyak\GE15657
Curated drosophilid orthologs	Dpse\GA14297   Dvir\Met (Wilson et al., 1999)
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • 247693 Daphnia pulex (daphnia) 214146 Nematostella vectensis (starlet anemone) XP_001606775.1 Nasonia vitripennis (wasp) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • FBgn0233227 FBgn0188611 FBgn0168029 FBgn0110650
Stocks & Reagents
Stocks Listed in FlyBase ( 16 )
Bloomington	3472 Met1 3489 v1 MetN6 3487 v1 MetD29 26205 y1 v1; P{TRiP.JF02103}attP2 3480 y1 v1 Met3/Dp(1;Y)y+
Kyoto	107055 Met1 107064 v1 MetN6
VDRC	v100638 P{KK104562}VIE-260B v10801 w1118 P{GD4384}v10801 v45852 w1118; P{GD4384}v45852 v45853 w1118; P{GD4384}v45853 v45854 w1118; P{GD4384}v45854
	More stocks available...
Genomic Clones ( 2 )
	BACR26B05 BACR29A04 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 17 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	RE71296
Other clones	FI10506
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	HL03612 LD24801
Other clones	70108 108456 162573 214283.4 524721 EK038938 EK244864 EK266532 EN05814 EP08558 FGC001F07 FGM232B07 IP09271
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0002723 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 32114
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: Rst(1)JH CG1705 (Wang, 1999.11)
Source for database merge of
Additional comments	Six EMS-induced, X-linked mutations resistant to juvenile hormone reported by Arking and Vlach (1970) (now lost); resistance factors varied from 1.4 to 21.5 by their methods. Presumed to be alleles of the mutation described by Wilson and Fabian.
Other Comments
	Alleles of Rst(1)JH vary in their effects on both uni- and multivariate life history traits. (Flatt and Kawecki, 2004) Cloning and characterisation of Rst(1)JH. (Ashok et al., 1998) Mutations alter Juvenile hormone reception during late larval development. (Turner and Wilson, 1995) Molecular cloning and characterisation of the Met gene region. (Turner and Wilson, 1995) Rst(1)JH gene product performs a role in the regulation of insect larval molting and metamorphosis of the epidermis and the nervous system. (Riddiford, 1993) Distribution of juvenile hormone binding proteins between the cytosol and nucleus was studied by photoaffinity labelling with tritiated epoxyfarnesyl diazoacetate. An 85kD cytosolic juvenile hormone binding protein is suggested to be the same as that identified by Shemshedini et al., (JBC 265:1913--1918) alterations in which are correlated with mutations at this locus. (Shemshedini and Wilson, 1993) Located in 10C2-10D4 by deficiency mapping. (Wilson and Fabian, 1986)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_078571 RefSeq (Proteins) • NP_511126 DDBJ / EMBL / GenBank • AAC14350 AAF48071 AF034859 Entrez Gene - A searchable database of RefSeq genes. • 32114 UniProtKB/TrEMBL • O44118 Q9VYW2
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • PAS (IPR000014) Helix-loop-helix DNA-binding (IPR011598) PAC motif (IPR001610)
Linkouts
	BioGRID - A database of protein and genetic interactions • 58523 DroID - A comprehensive database of gene and protein interactions. • FBgn0002723 DRSC - Results from RNAi screens. • FBgn0002723 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0002723 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG1705-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0002723 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 32114 InParanoid A subset of ortholog calls from InParanoid. • 247693 Daphnia pulex (daphnia) 214146 Nematostella vectensis (starlet anemone) XP_001606775.1 Nasonia vitripennis (wasp) Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /sturtevant/res-jh1.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 32114 modMine - Data generated by the modENCODE project. • FBgn0002723 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • FBgn0233227 FBgn0188611 FBgn0168029 FBgn0110650
Synonyms & Secondary IDs ( 15 )
Reported As
Symbol Synonym	bHLHe59 (Stevens et al., 2008) CG1705 (Jindra and Flatt, 2007.4.13, Stevens et al., 2008, Stevens et al., 2008, Perkins et al., 2009.3.31, Keleman et al., 2009.8.5, Baumann et al., 2010) DmMet (Bitra and Palli, 2009, Wang et al., 2007, Li et al., 2007) Met (Wilson et al., 2006, Flatt and Kawecki, 2004, Flatt and Kawecki, 2005, ffrench-Constant et al., 2004, Adam et al., 2003, Wilson et al., 2002, Wilson et al., 2001, Gilbert et al., 2000, Pursley et al., 2000, Gruntenko et al., 2000, Gruntenko et al., 2000, Gruntenko et al., 1999, Wilson et al., 1999, Pursley et al., 1999, Hahn, 1998, Wilson and Ashok, 1998, Restifo and Wilson, 1998, Feyereisen, 1998, Ashok et al., 1998, Wilson et al., 1998, Wilson et al., 1997, Wilson, 1996, Turner and Wilson, 1995, Henrich and Brown, 1995, Morton, 1993, Segal, 1993, Minkoff and Wilson, 1992, Riddiford and Ashburner, 1991, Shemshedini and Wilson, 1990, Shemshedini et al., 1990, Wilson and Fabian, 1986, Flatt et al., 2006, Riddiford, 1993, Flatt et al., 2005, Wilson et al., 2003, Jindra and Flatt, 2007.4.13, Wilson et al., 2006, Jindra and Konopova, 2008, Godlewski et al., 2006, Barry et al., 2008, Liu et al., 2009, Flatt et al., 2008, Wang et al., 2007, Miura et al., 2005, Riddiford et al., 2010, Zhang et al., 2010, Baumann et al., 2010, Li et al., 2011, Dubrovsky et al., 2011) met (Wheeler and Nijhout, 2003, ffrench-Constant et al., 2006) MET (Greb-Markiewicz et al., 2011) Mett   Rst(1)JH (Jindra and Flatt, 2007.4.13, Ledent and Vervoort, 2001, Schneider, 2000, Lindsley and Zimm, 1992, Jeon et al., 2008)
Name Synonym	juvenile hormone resistance (Wilson, 1997.11.17) methoprene-tolerant (Bitra and Palli, 2009, Flatt et al., 2006, Jindra and Flatt, 2007.4.13, Flatt et al., 2008, Dubrovsky et al., 2011) Methoprene-tolerant (Beno et al., 2005, ffrench-Constant et al., 2004, Wheeler and Nijhout, 2003, Wilson et al., 2002, Wilson et al., 1998, Wilson et al., 1997, Wilson, 1996, Turner and Wilson, 1995, Flatt et al., 2005, Wilson et al., 2003, Jindra and Flatt, 2007.4.13, Wilson et al., 2006, Godlewski et al., 2006, Wang et al., 2007, Liu et al., 2009, Barry et al., 2008, Riddiford et al., 2010) methoprene tolerant (Morton, 1993) Methoprene-tolerant protein (Greb-Markiewicz et al., 2011) Resistance (1) Juvenile H (Lindsley and Zimm, 1992) Resistance to Juvenile Hormone (Flatt et al., 2005, Jindra and Flatt, 2007.4.13)
Secondary FlyBase IDs
References ( 82 )
Generate a list of	All references relating to this gene ( 82 )
List References by type	Research paper  ( 42 ) Supplementary material  ( 4 ) Review  ( 12 ) Personal communication to FlyBase  ( 5 ) Abstract  ( 13 ) FlyBase analysis  ( 1 ) DNA/RNA sequence record  ( 1 ) Book  ( 1 ) Curated genome annotation  ( 2 ) Automatic genome annotation  ( 1 )
Recent research papers ( 8 )
	Dubrovsky et al., 2011, J. Biol. Chem. 286(38): 33689--33700 The Drosophila FTZ-F1 Nuclear Receptor Mediates Juvenile Hormone Activation of E75A Gene Expression through an Intracellular Pathway. [FBrf0215576] Greb-Markiewicz et al., 2011, Molec. Cell. Endocrinol. 345(1-2): 16--26 Sequences that direct subcellular traffic of the Drosophila methoprene-tolerant protein (MET) are located predominantly in the PAS domains. [FBrf0215249] Huang et al., 2011, Development 138(11): 2283--2291 DPP-mediated TGF{beta} signaling regulates juvenile hormone biosynthesis by activating the expression of juvenile hormone acid methyltransferase. [FBrf0213687] Li et al., 2011, Proc. Natl. Acad. Sci. U.S.A. 108(2): 638--643 Heterodimer of two bHLH-PAS proteins mediates juvenile hormone-induced gene expression. [FBrf0212776] Baumann et al., 2010, J. Insect Physiol. 56(10): 1445--1455 Evolutionary divergence of the paralogs Methoprene tolerant (Met) and germ cell expressed (gce) within the genus Drosophila. [FBrf0211455] Baumann et al., 2010, Genetics 185(4): 1327--1336 Paralogous Genes Involved in Juvenile Hormone Action in Drosophila melanogaster. [FBrf0211496] Riddiford et al., 2010, Development 137(7): 1117--1126 A role for juvenile hormone in the prepupal development of Drosophila melanogaster. [FBrf0210225] Zhang et al., 2010, Biochem. Biophys. Res. Commun. 401(2): 182--187 Drosophila CG10527 mutants are resistant to juvenile hormone and its analog methoprene. [FBrf0212071] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...