FlyBase Gene Report: Dmel\mago

General Information

Symbol

Dmel\mago

Species

D. melanogaster

Name

mago nashi

Annotation Symbol

CG9401

Feature Type

protein_coding_gene

FlyBase ID

FBgn0002736

Gene Model Status

Stock Availability

10 publicly available

Gene Snapshot

mago nashi (mago) encodes a protein that is part of the exon junction complex, which is required for post-transcriptional processes such as pre-mRNA splicing, RNA localization and nonsense-mediated decay. It is involved in germline development, germplasm assembly and photoreceptor differentiation. [Date last reviewed: 2019-03-14]

Other Summaries

Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

mgn, l(2)57Ca

Key Links

Genomic Location

Cytogenetic map

Sequence location

2R:21,128,577..21,129,737 [+]

Recombination map

2-93

RefSeq locus

NT_033778 REGION:21128577..21129737

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (20 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables protein binding

inferred from physical interaction with UniProtKB:P82804

(assigned by UniProt )

(Bono et al., 2004, Forler et al., 2003)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (13 terms)

Terms Based on Experimental Evidence (7 terms)

CV Term

Evidence

References

involved_in negative regulation of transposition, DNA-mediated

inferred from mutant phenotype

(Hayashi et al., 2014)

involved_in oocyte microtubule cytoskeleton polarization

inferred from mutant phenotype

(Micklem et al., 1997)

involved_in oogenesis

inferred from mutant phenotype

(Parma et al., 2007)

involved_in positive regulation of gene expression

inferred from mutant phenotype

(Ashton-Beaucage et al., 2014)

involved_in protein localization

inferred from mutant phenotype

(Mohr et al., 2001)

inferred from physical interaction with FLYBASE:tsu; FB:FBgn0033378

(Mohr et al., 2001)

involved_in regulation of pole plasm oskar mRNA localization

inferred from mutant phenotype

(Micklem et al., 1997)

involved_in RNA splicing

inferred from mutant phenotype

(Hayashi et al., 2014, Ashton-Beaucage et al., 2010, Roignant and Treisman, 2010)

Terms Based on Predictions or Assertions (7 terms)

CV Term

Evidence

References

involved_in maintenance of pole plasm mRNA location

traceable author statement

(Farina and Singer, 2002)

involved_in microtubule cytoskeleton organization

traceable author statement

(Cooperstock and Lipshitz, 1997)

involved_in mRNA splicing, via spliceosome

inferred by curator from GO:0071011,GO:0071013

(Herold et al., 2009)

involved_in oocyte microtubule cytoskeleton organization

traceable author statement

(Johnstone and Lasko, 2001)

involved_in pole plasm assembly

traceable author statement

(Mahowald, 2001, Wylie, 1999)

involved_in pole plasm oskar mRNA localization

traceable author statement

(Farina and Singer, 2002, Tekotte and Davis, 2002)

involved_in RNA splicing

inferred from biological aspect of ancestor with PANTHER:PTN000290902

(assigned by GO_Central )

(Gaudet et al., 2011)

Cellular Component (6 terms)

Terms Based on Experimental Evidence (6 terms)

CV Term

Evidence

References

part_of catalytic step 2 spliceosome

inferred from high throughput direct assay

(Herold et al., 2009)

located_in cytoplasm

inferred from direct assay

(Parma et al., 2007, Mohr et al., 2001)

part_of exon-exon junction complex

inferred from physical interaction with FLYBASE:tsu; FB:FBgn0033378

(Lewandowski et al., 2010)

located_in nucleus

inferred from direct assay

(Parma et al., 2007, Le Hir et al., 2001, Mohr et al., 2001)

located_in pole plasm

inferred from direct assay

(Palacios et al., 2004)

part_of precatalytic spliceosome

inferred from high throughput direct assay

(Herold et al., 2009)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

part_of catalytic step 2 spliceosome

inferred from biological aspect of ancestor with PANTHER:PTN000973970

(assigned by GO_Central )

(Gaudet et al., 2011)

part_of exon-exon junction complex

inferred from biological aspect of ancestor with PANTHER:PTN000290902

(assigned by GO_Central )

(Gaudet et al., 2011)

Gene Group (FlyBase)

SPLICEOSOME COMPLEX C

SPLICEOSOME COMPLEX P

Protein Family (UniProt)

Belongs to the mago nashi family. (P49028)

Protein Signatures (InterPro)

Summaries

Gene Group (FlyBase)

SPLICEOSOME COMPLEX C -: Nuclear pre-mRNA splicing is catalyzed by the spliceosome which assembles in a step-wise manner. Complex B undergoes a number of structural rearrangements and U4 and U1 snRNPs dissociate to generate complex C, the catalytic spliceosome. (Adapted from PMID:24452469 and PMID:23118483).
SPLICEOSOME COMPLEX P -: Nuclear pre-mRNA splicing is catalyzed by the spliceosome which assembles in a step-wise manner. Complex P, the post-spliceosomal complex, is formed after the catalytic removal of the intron by complex C. Complex P contains the lariat intron and spliced exons. (Adapted from PMID:24452469 and PMID:23118483).

Protein Function (UniProtKB)

Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs (PubMed:14973490, PubMed:24967911). Involved in exon definition of genes containing long introns, including the rolled/MAPK gene (PubMed:20946982, PubMed:20946983). The mago-tsu heterodimer interacts with the EJC key regulator Pym leading to EJC disassembly in the cytoplasm (PubMed:24967911). Has a role in oskar mRNA localization to the posterior pole of the developing oocyte, and may also be involved in polarization of the oocyte microtubule cytoskeleton (PubMed:8026338, PubMed:9272960).

(UniProt, P49028)

Summary (Interactive Fly)

novel protein involved in oogenesis - mutation results from the failure of nuclear migration to the anterodorsal cortex during oogenesis - part of the exon junction complex, which is required for post-transcriptional processes such as pre-mRNA splicing, RNA localization and nonsense-mediated decay - involved in germline development, germplasm assembly and photoreceptor differentiation

Interactive Fly

Gene Model and Products

Number of Transcripts

1

Number of Unique Polypeptides

1

Please see the JBrowse view of Dmel\mago for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.51

Sequence Ontology: Class of Gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

FBtr0071569

1103

147

Additional Transcript Data and Comments

Reported size (kB)

1.1, 0.7 (northern blot)

(Newmark and Boswell, 1994)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

FBpp0071497

17.3

147

6.01

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

147 (aa); 17 (kD predicted)

(Newmark and Boswell, 1994)

Comments

External Data

Subunit Structure (UniProtKB)

Heterodimer with tsu/RBM8A (PubMed:12704080, PubMed:12730685, PubMed:14968132). Part of the mRNA splicing-dependent exon junction complex (EJC) complex; the core complex contains btz/CASC3, eIF4AIII, mago and tsu/RBM8A (PubMed:14973490). Interacts with Pym (via N-terminus); the interaction is direct (PubMed:24967911, PubMed:14968132). Interacts with eIF4AIII (PubMed:14973490).

(UniProt, P49028)

Crossreferences

InterPro - A database of protein families, domains and functional sites

PDB - An information portal to biological macromolecular structures

1HL6
1OO0

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

P49028

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\mago using the Feature Mapper tool.

External Data

Crossreferences

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

antennal anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

central brain anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

dorsal head epidermis anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

embryonic stage 9 -- 10

antennal primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

central brain primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

visual primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

dorsal head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

lateral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

ventral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

anterior endoderm

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

posterior endoderm

(Fisher et al., 2012)

ventral ectoderm

(Fisher et al., 2012)

ventral nerve cord primordium

(Fisher et al., 2012)

embryonic stage 11 -- 12

anterior midgut primordium

(Fisher et al., 2012)

central brain primordium

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

posterior midgut primordium

(Fisher et al., 2012)

ventral nerve cord primordium

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic brain

(Fisher et al., 2012)

embryonic/larval midgut

(Fisher et al., 2012)

embryonic/larval nervous system

(Fisher et al., 2012)

(Fisher et al., 2012)

ventral nerve cord

(Fisher et al., 2012)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage & oogenesis

(Newmark and Boswell, 1994)

(Newmark and Boswell, 1994)

embryonic stage -- adult stage

(Newmark and Boswell, 1994)

embryonic stage 1 -- 16

(Newmark and Boswell, 1994)

Additional Descriptive Data

mago transcripts are expressed throughout development and are present in both adult males and females. They are uniformly expressed throughout the nurse cell-oocyte complex during early oogenesis, are abundant in nurse cells at stage 10, and appear to be uniformly distributed throughout the embryo by the time of egg deposition.

(Newmark and Boswell, 1994)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

epitope tag

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage | female & oogenesis stage S9

• nucleus

(van Eeden et al., 2001)

ring canal | surrounding

(van Eeden et al., 2001)

oocyte | posterior

• nucleus

Comment: tightly localized

(van Eeden et al., 2001)

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

oogenesis & adult stage | female

(Parma et al., 2007)

Additional Descriptive Data

mago colocalizes with orb throughout the germarium.

(Parma et al., 2007)

mago protein is localized to the nuclei of nurse cells in the developing egg chamber. mago also colocalizes with btz in a dense cloud around the ring canals, and at the posterior pole of stage 9 oocytes.

(van Eeden et al., 2001)

Marker for

Subcellular Localization

CV Term

Evidence

References

part_of catalytic step 2 spliceosome

inferred from high throughput direct assay

(Herold et al., 2009)

located_in cytoplasm

inferred from direct assay

(Parma et al., 2007, Mohr et al., 2001)

part_of exon-exon junction complex

inferred from physical interaction with FLYBASE:tsu; FB:FBgn0033378

(Lewandowski et al., 2010)

located_in nucleus

inferred from direct assay

(Parma et al., 2007, Le Hir et al., 2001, Mohr et al., 2001)

located_in pole plasm

inferred from direct assay

(Palacios et al., 2004)

part_of precatalytic spliceosome

inferred from high throughput direct assay

(Herold et al., 2009)

Expression Deduced from Reporters

Reporter: P{GFPmago17.1}

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage | female & oogenesis stage S9

• nucleus

(van Eeden et al., 2001)

ring canal | surrounding

(van Eeden et al., 2001)

oocyte | posterior

• nucleus

Comment: tightly localized

(van Eeden et al., 2001)

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\mago in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\mago

View exonic expression by tissue for Dmel\mago

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0002736

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0002736

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG9401-RA

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG9401

Flygut - An atlas of the Drosophila adult midgut

FBgn0002736

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\mago

Stages(s) 1-3
Stages(s) 11-12
Stages(s) 13-16

Image Based Resources

Alleles, Insertions, and Transgenic Constructs

Classical and Insertion Alleles ( 14 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 14 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of mago

Transgenic constructs containing regulatory region of mago

Deletions and Duplications ( 14 )

Not disrupted in

(O'Donnell et al., 1989)

(O'Donnell et al., 1989)

(O'Donnell et al., 1989)

(O'Donnell et al., 1989)

(O'Donnell et al., 1989)

(O'Donnell et al., 1989)

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal (with Df(2R)AA21)

mago³

lethal | female limited, with Scer\GAL4^dome-PG14

mago^HM05142

lethal | maternal effect | recessive | cold sensitive

mago¹

lethal | recessive

lethal | recessive | heat sensitive

mago¹

lethal - all die before end of embryonic stage | maternal effect

mago³

lethal - all die before end of embryonic stage | maternal effect | recessive | heat sensitive

lethal - all die during P-stage, with Scer\GAL4^Mef2.PR

mago^GD12556

partially lethal - majority die, with Scer\GAL4^pnr-MD237

mago^GD12556

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

mago^GD12556

viable, with Scer\GAL4^tin.CΔ4

mago^GD12556

Sterility

Allele

female sterile, with Scer\GAL4^VP16.otu

mago^HM05142

female sterile | maternal effect | recessive

mago¹

male sterile | maternal effect | recessive

mago¹

mago^ETo2

Other Phenotypes

Allele

body color defective, with Scer\GAL4^pnr-MD237

mago^GD12556

short lived | heat sensitive, with Scer\GAL4^tin.CΔ4

mago^GD12556

some die during embryonic stage | maternal effect

mago³

some die during embryonic stage | recessive | maternal effect | recessive | heat sensitive

some die during pupal stage, with Scer\GAL4^pnr-MD237

mago^GD12556

visible, with Scer\GAL4^pnr-MD237

mago^GD12556

visible | recessive | heat sensitive

mago¹

Phenotype manifest in

Allele

dorsal appendage

mago¹

mago¹

egg | germline clone

egg chamber | germline clone

mago¹

mago¹

mago^unspecified

fusome | germline clone

mago^SHL1

mesothoracic tergum, with Scer\GAL4^pnr-MD237

mago^GD12556

mago¹

microtubule & egg chamber

mago¹

microtubule & oocyte

mago¹

oocyte nucleus | germline clone

posterior aeropyle

mago¹

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (2)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

14 of 15

Yes

Yes

13 of 15

No

Yes

1

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (2)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

14 of 15

Yes

Yes

12 of 15

No

Yes

Rattus norvegicus (Norway rat) (2)

12 of 13

Yes

Yes

10 of 13

No

Yes

Xenopus tropicalis (Western clawed frog) (1)

13 of 12

Yes

Yes

Danio rerio (Zebrafish) (1)

14 of 15

Yes

Yes

Caenorhabditis elegans (Nematode, roundworm) (1)

13 of 15

Yes

Yes

1

Arabidopsis thaliana (thale-cress) (1)

9 of 9

Yes

Yes

Saccharomyces cerevisiae (Brewer's yeast) (0)

No records found.

Schizosaccharomyces pombe (Fission yeast) (1)

11 of 12

Yes

Yes

Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG09190HJB )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila suzukii

Spotted wing Drosophila

Dsuz\DS10_00002753

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Drosophila persimilis

Drosophila willistoni

Drosophila virilis

Drosophila mojavensis

Drosophila grimshawi

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150DGQ )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Glossina morsitans

Tsetse fly

Gmor\GMOY000684

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_11217

Mayetiola destructor

Hessian fly

Mdes\Mdes009754

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL011544

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL010453

Anopheles darlingi

American malaria mosquito

Adar\ADAC003131

Anopheles gambiae

Malaria mosquito

Agam\AGAP010755

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ002949

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0GBK )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA003398

Danaus plexippus

Monarch butterfly

Dple\DPOGS208733-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL015246

Apis florea

Little honeybee

Aflo\5130686980

Apis mellifera

Western honey bee

Bombus impatiens

Common eastern bumble bee

Bombus terrestris

Buff-tailed bumblebee

Linepithema humile

Argentine ant

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:00214c

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG004040

Dendroctonus ponderosae

Mountain pine beetle

Tribolium castaneum

Red flour beetle

Pediculus humanus

Human body louse

Phum\PHUM291670

Rhodnius prolixus

Kissing bug

Rpro\RPRC010985

Cimex lectularius

Bed bug

Clec\CLEC004994

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI000063

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_04496

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0GC9 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR008803

Ixodes scapularis

Black-legged tick

Isca\ISCW015637

Stegodyphus mimosarum

African social velvet spider

Smim\KFM63953.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur34g01000

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_299767

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0UKA )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000002708

Gallus gallus

Domestic chicken

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (0)

No records found.

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

MAGOHB; mago homolog B, exon junction complex subunit

14 of 15

MAGOH; mago homolog, exon junction complex subunit

13 of 15

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Dmel gene

Ortholog showing functional complementation

Supporting References

(Newmark et al., 1997)

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

anti tag coimmunoprecipitation, western blot, anti bait coimmunoprecipitation, pull down, molecular weight estimation by staining

(Obrdlik et al., 2019, Ghosh et al., 2014, Palacios et al., 2004)

pull down, molecular weight estimation by staining, anti tag coimmunoprecipitation, western blot, x-ray crystallography, experimental knowledge based, anti bait coimmunoprecipitation

(Ghosh et al., 2014, Guruharsha et al., 2011, Bono et al., 2010, Bono et al., 2004)

pull down, molecular weight estimation by staining, x-ray crystallography

(Bono et al., 2010)

anti tag coimmunoprecipitation, western blot

(Lewandowski et al., 2010)

anti tag coimmunoprecipitation, anti tag western blot, western blot, pull down, molecular weight estimation by staining, molecular sieving, x-ray crystallography, experimental knowledge based

(Obrdlik et al., 2019, Ghosh et al., 2014, Guruharsha et al., 2011, Bono et al., 2010, Lewandowski et al., 2010, Bono et al., 2004, Fribourg et al., 2003, Shi and Xu, 2003)

RNA-protein

Physical Interaction

Assay

References

anti tag coimmunoprecipitation, quantitative reverse transcription pcr

(Saulière et al., 2010)

anti tag coimmunoprecipitation, quantitative reverse transcription pcr

(Saulière et al., 2010)

anti tag coimmunoprecipitation, primer specific pcr

(Ghosh et al., 2014)

anti tag coimmunoprecipitation, autoradiography

(Ghosh et al., 2012)

anti tag coimmunoprecipitation, primer specific pcr

(Ghosh et al., 2014)

anti tag coimmunoprecipitation, primer specific pcr

(Ghosh et al., 2014)

anti tag coimmunoprecipitation, primer specific pcr, autoradiography

(Ghosh et al., 2014, Ghosh et al., 2012)

anti tag coimmunoprecipitation, quantitative reverse transcription pcr

(Saulière et al., 2010)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

suppressible

(Morais-de-Sá et al., 2013)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

enhanceable

(Ashton-Beaucage et al., 2010)

suppressible

(Ashton-Beaucage et al., 2010)

suppressible

(Ashton-Beaucage et al., 2010)

enhanceable

(Ashton-Beaucage et al., 2010)

enhanceable

(Jankovics et al., 2001)

External Data

Subunit Structure (UniProtKB)

Heterodimer with tsu/RBM8A (PubMed:12704080, PubMed:12730685, PubMed:14968132). Part of the mRNA splicing-dependent exon junction complex (EJC) complex; the core complex contains btz/CASC3, eIF4AIII, mago and tsu/RBM8A (PubMed:14973490). Interacts with Pym (via N-terminus); the interaction is direct (PubMed:24967911, PubMed:14968132). Interacts with eIF4AIII (PubMed:14973490).

(UniProt, P49028 )

Linkouts

BioGRID - A database of protein and genetic interactions.

63039

DroID - A comprehensive database of gene and protein interactions.

FBgn0002736

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

37402

MIST (genetic) - An integrated Molecular Interaction Database

mago

MIST (protein-protein) - An integrated Molecular Interaction Database

mago

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

2R

Recombination map

2-93

Cytogenetic map

Sequence location

2R:21,128,577..21,129,737 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

57C2-57C2

Limits computationally determined from genome sequence between P{PZ}king-tubby⁰²⁸²¹&P{lacW}Xbp1^k13803 and P{EP}CG4266^EP2258

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

57B20-57C2

(Newmark, 1993.11.17)

Experimentally Determined Recombination Data

Location

2-93

(FlyBase, 2016)

2-100.3

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (10)

28931

y¹ v¹; P{TRiP.HM05142}attP2

51289

w^*; P{neoFRT}42D mago³ shn³/CyO

35453

y¹ sc^* v¹ sev²¹; P{TRiP.GL00378}attP2

82653

y¹ sc^* v¹ sev²¹; P{TKO.GS04117}attP40

55260

y¹ sc^* v¹ sev²¹; P{TRiP.HMC03947}attP40

51304

P{neoFRT}42D arr² mago³/T(2;3)TSTL14, SM5: TM6B, Tb¹

51303

P{neoFRT}42D mam¹⁰ mago³/T(2;3)TSTL14, SM5: TM6B, Tb¹

v330456

P{VSH330456}attP40

v28132

w¹¹¹⁸; P{GD12556}v28132

v318325

PBac{fTRG01045.sfGFP-TVPTBF}VK00033

Genomic Clones (18)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (70)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

RE14116

Other clones

DPiM_RE14116

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0002736

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0002736

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

37402

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Choudhury et al., 2016)

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for database merge of

Additional comments

Other Comments

mago has a role in the splicing regulation of genes containing long introns.

(Ashton-Beaucage et al., 2010)

mago has a role in the splicing regulation of heterochromatic genes containing long introns.

(Roignant and Treisman, 2010)

mago is required for female germline stem cell differentiation.

(Parma et al., 2007)

dsRNA has been made from templates generated with primers directed against this gene.

(Roignant et al., 2006)

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

The mago/tsu physical interaction is conserved, occurs in vitro and in vivo and is not RNA mediated. mago and tsu gene products localize within nuclear speckles. mago/tsu heterodimers associate with spliced mRNAs carrying an exon-exon junction complex.

(Le Hir et al., 2001)

mago and tsu are required for cell viability.

(Le Hir et al., 2001)

mago plays two essential roles in Drosophila axis formation: it is required downstream of the signal from the posterior follicle cells for the polarisation of the oocyte microtubule cytoskeleton, and it has a second, independent role in the localisation of osk mRNA to the posterior of the oocyte.

(Micklem et al., 1997)

mago protein must be localised within the posterior pole plasm for germ-plasm assembly.

(Newmark et al., 1997)

Distribution of tud protein in mutant embryos has been studied.

(Bardsley et al., 1993)

mago gene product is a component of the posterior determinative system, required during oogenesis for germ cell determination and delineation of the longitudinal axis of the embryo.

(Boswell et al., 1991)

Origin and Etymology

Discoverer

Etymology

"mago nashi" is Japanese for "without grandchildren".

(Newmark and Boswell, 1994)

Identification

External Crossreferences and Linkouts ( 43 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

37402

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P49028

Other crossreferences

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0002736

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0002736

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG9401

Flygut - An atlas of the Drosophila adult midgut

FBgn0002736

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

37402

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG9401

modMine - A data warehouse for the modENCODE project

FBgn0002736

PDB - An information portal to biological macromolecular structures

1HL6
1OO0

Linkouts

BioGRID - A database of protein and genetic interactions.

63039

DPiM - Drosophila Protein interaction map

FBgn0002736

DroID - A comprehensive database of gene and protein interactions.

FBgn0002736

DRSC - Results frm RNAi screens

FBgn0002736

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0002736

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG9401-RA

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0002736

FlyMine - An integrated database for Drosophila genomics

FBgn0002736

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

37402

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

MIST (genetic) - An integrated Molecular Interaction Database

mago

MIST (protein-protein) - An integrated Molecular Interaction Database

mago

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Synonyms and Secondary IDs (16)

Reported As

Symbol Synonym

CG9401

(Meadows, 2018.4.4, Hosono et al., 2015, Ni et al., 2011.7.19, Ni et al., 2010.12.1, Herold et al., 2009, Perkins et al., 2009, Gandhi et al., 2007)

Dm MGN

(Le Hir et al., 2001)

DmMago

(Palacios, 2002)

MAGO

(Choudhury et al., 2016)

MAGOH

(Behm-Ansmant and Izaurralde, 2006)

MGN

(Le Hir et al., 2001)

Mago

(Obrdlik et al., 2019, Gáspár et al., 2017, Le Thomas et al., 2013, Ghosh et al., 2012, Bono et al., 2010, Lewandowski et al., 2010, Saulière et al., 2010, Minakhina and Steward, 2005, Bono et al., 2004, Fribourg et al., 2003)

Magoh

(Le Hir et al., 2003)

l(2)57Ca

(O'Donnell et al., 1989)

mago

(Gao et al., 2019, Trcek and Lehmann, 2019, Whittle and Extavour, 2019, Wang et al., 2018, Ashton-Beaucage and Therrien, 2017, Garcia-Garcia et al., 2017, Transgenic RNAi Project members, 2017-, Ashton-Beaucage et al., 2016, Dennis et al., 2016, Malartre, 2016, Sarov et al., 2016, Yan and Perrimon, 2015, Ashton-Beaucage et al., 2014, Ghosh et al., 2014, Hayashi et al., 2014, Czech et al., 2013, Handler et al., 2013, Handler et al., 2013, Morais-de-Sá et al., 2013, Treisman, 2013.10.7, Ashton-Beaucage and Therrien, 2011, Friedman et al., 2011, Ashton-Beaucage et al., 2010, Roignant and Treisman, 2010, Baker et al., 2009, Firth and Baker, 2009, Herold et al., 2009, Tuxworth et al., 2009, Okamura et al., 2008, Zimyanin et al., 2008, Roignant et al., 2007, Zimyanin et al., 2007, Doerflinger et al., 2006, Irion et al., 2006, Wang et al., 1994)

mgn

(Foe et al., 1993, Newmark, 1993.11.17, Lindsley and Zimm, 1986)

Name Synonyms

Mago Nashi

(Lewandowski et al., 2010, Nie et al., 2009, Parma et al., 2007)

Mago nashi

(Zimyanin et al., 2008, Hachet and Ephrussi, 2004)

mago nashi

(Jakšić and Schlötterer, 2016, Malartre, 2016, Handler et al., 2013, Roignant and Treisman, 2010, Tuxworth et al., 2009)

mago nishi

(Roignant et al., 2006)

mago-nashi

(Tuxworth et al., 2009, Hachet and Ephrussi, 2002)

Secondary FlyBase IDs

FBgn0002095

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (168)