FB2019_06, released Dec 19, 2019

Gene: Dmel\mago

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General Information
Symbol
Dmel\mago
Species
D. melanogaster
Name
mago nashi
Annotation Symbol
CG9401
Feature Type
protein_coding_gene
FlyBase ID
FBgn0002736
Gene Model Status
Current
Stock Availability
10 publicly available
Gene Snapshot
mago nashi (mago) encodes a protein that is part of the exon junction complex, which is required for post-transcriptional processes such as pre-mRNA splicing, RNA localization and nonsense-mediated decay. It is involved in germline development, germplasm assembly and photoreceptor differentiation. [Date last reviewed: 2019-03-14]
Other Summaries
Also Known As
mgn, l(2)57Ca
Key Links
Genomic Location
Cytogenetic map
57C2-57C2
Sequence location
2R:21,128,577..21,129,737 [+]
Recombination map
2-93
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the mago nashi family. (P49028)
Protein Signatures (InterPro)
Molecular Function (GO)
[Detailed GO annotations]
Experimental Evidence
protein binding
Predictions / Assertions
-
Summaries
Gene Group (FlyBase)
SPLICEOSOME COMPLEX C -
Nuclear pre-mRNA splicing is catalyzed by the spliceosome which assembles in a step-wise manner. Complex B undergoes a number of structural rearrangements and U4 and U1 snRNPs dissociate to generate complex C, the catalytic spliceosome. (Adapted from PMID:24452469 and PMID:23118483).
SPLICEOSOME COMPLEX P -
Nuclear pre-mRNA splicing is catalyzed by the spliceosome which assembles in a step-wise manner. Complex P, the post-spliceosomal complex, is formed after the catalytic removal of the intron by complex C. Complex P contains the lariat intron and spliced exons. (Adapted from PMID:24452469 and PMID:23118483).
Protein Function (UniProtKB)
Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs (PubMed:14973490, PubMed:24967911). Involved in exon definition of genes containing long introns, including the rolled/MAPK gene (PubMed:20946982, PubMed:20946983). The mago-tsu heterodimer interacts with the EJC key regulator Pym leading to EJC disassembly in the cytoplasm (PubMed:24967911). Has a role in oskar mRNA localization to the posterior pole of the developing oocyte, and may also be involved in polarization of the oocyte microtubule cytoskeleton (PubMed:8026338, PubMed:9272960).
(UniProt, P49028)
Summary (Interactive Fly)
novel protein involved in oogenesis - mutation results from the failure of nuclear migration to the anterodorsal cortex during oogenesis - part of the exon junction complex, which is required for post-transcriptional processes such as pre-mRNA splicing, RNA localization and nonsense-mediated decay - involved in germline development, germplasm assembly and photoreceptor differentiation
Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the GBrowse view of Dmel\mago or the JBrowse view of Dmel\mago for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model
Gene model reviewed during 5.51
Sequence Ontology: Class of Gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
mago-RA
FBtr0071569
NM_057288
1103
147
Additional Transcript Data and Comments
Reported size (kB)
1.1, 0.7 (northern blot)
(Newmark and Boswell, 1994)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
mago-PA
FBpp0071497
17.3
147
6.01
NP_476636
AAF46677
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)
147 (aa); 17 (kD predicted)
(Newmark and Boswell, 1994)
Comments
External Data
Subunit Structure (UniProtKB)
Heterodimer with tsu/RBM8A (PubMed:12704080, PubMed:12730685, PubMed:14968132). Part of the mRNA splicing-dependent exon junction complex (EJC) complex; the core complex contains btz/CASC3, eIF4AIII, mago and tsu/RBM8A (PubMed:14973490). Interacts with Pym (via N-terminus); the interaction is direct (PubMed:24967911, PubMed:14968132). Interacts with eIF4AIII (PubMed:14973490).
(UniProt, P49028)
Crossreferences
InterPro - A database of protein families, domains and functional sites
PDB - An information portal to biological macromolecular structures
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\mago using the Feature Mapper tool.

External Data
Crossreferences
Linkouts
Gene Ontology (20 terms)
Molecular Function (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
protein binding
inferred from physical interaction with UniProtKB:P82804
(assigned by UniProt )
(Bono et al., 2004, Forler et al., 2003)
Terms Based on Predictions or Assertions (0 terms)
Biological Process (13 terms)
Terms Based on Experimental Evidence (7 terms)
CV Term
Evidence
References
negative regulation of transposition, DNA-mediated
inferred from mutant phenotype
(Hayashi et al., 2014)
oocyte microtubule cytoskeleton polarization
inferred from mutant phenotype
(Micklem et al., 1997)
oogenesis
inferred from mutant phenotype
(Parma et al., 2007)
positive regulation of gene expression
inferred from mutant phenotype
(Ashton-Beaucage et al., 2014)
protein localization
inferred from mutant phenotype
(Mohr et al., 2001)
inferred from physical interaction with FLYBASE:tsu; FB:FBgn0033378
(Mohr et al., 2001)
regulation of pole plasm oskar mRNA localization
inferred from mutant phenotype
(Micklem et al., 1997)
RNA splicing
inferred from mutant phenotype
(Hayashi et al., 2014, Ashton-Beaucage et al., 2010, Roignant and Treisman, 2010)
Terms Based on Predictions or Assertions (7 terms)
CV Term
Evidence
References
maintenance of pole plasm mRNA location
traceable author statement
(Farina and Singer, 2002)
microtubule cytoskeleton organization
traceable author statement
(Cooperstock and Lipshitz, 1997)
mRNA splicing, via spliceosome
inferred by curator from GO:0071011,GO:0071013
(Herold et al., 2009)
oocyte microtubule cytoskeleton organization
traceable author statement
(Johnstone and Lasko, 2001)
pole plasm assembly
traceable author statement
(Mahowald, 2001, Wylie, 1999)
pole plasm oskar mRNA localization
traceable author statement
(Farina and Singer, 2002, Tekotte and Davis, 2002)
RNA splicing
inferred from biological aspect of ancestor with PANTHER:PTN000290902
(assigned by GO_Central )
(Gaudet et al., 2011)
Cellular Component (6 terms)
Terms Based on Experimental Evidence (6 terms)
CV Term
Evidence
References
catalytic step 2 spliceosome
inferred from high throughput direct assay
(Herold et al., 2009)
cytoplasm
inferred from direct assay
(Parma et al., 2007, Mohr et al., 2001)
exon-exon junction complex
inferred from physical interaction with FLYBASE:tsu; FB:FBgn0033378
(Lewandowski et al., 2010)
nucleus
inferred from direct assay
(Parma et al., 2007, Le Hir et al., 2001, Mohr et al., 2001)
pole plasm
inferred from direct assay
(Palacios et al., 2004)
precatalytic spliceosome
inferred from high throughput direct assay
(Herold et al., 2009)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
catalytic step 2 spliceosome
inferred from biological aspect of ancestor with PANTHER:PTN000973970
(assigned by GO_Central )
(Gaudet et al., 2011)
exon-exon junction complex
inferred from biological aspect of ancestor with PANTHER:PTN000290902
(assigned by GO_Central )
(Gaudet et al., 2011)
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 1 -- 3
organism

Comment: maternally deposited

(Fisher et al., 2012)
embryonic stage 4 -- 6
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 7 -- 8
antennal anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)
central brain anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)
dorsal head epidermis anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)
visual anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)
embryonic stage 9 -- 10
antennal primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
central brain primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
visual primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
dorsal head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
lateral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
ventral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
anterior endoderm
(Fisher et al., 2012)
organism | ubiquitous
(Fisher et al., 2012)
posterior endoderm
(Fisher et al., 2012)
ventral ectoderm
(Fisher et al., 2012)
ventral nerve cord primordium
(Fisher et al., 2012)
embryonic stage 11 -- 12
anterior midgut primordium
(Fisher et al., 2012)
central brain primordium
(Fisher et al., 2012)
organism | ubiquitous
(Fisher et al., 2012)
posterior midgut primordium
(Fisher et al., 2012)
ventral nerve cord primordium
(Fisher et al., 2012)
embryonic stage 13 -- 16
embryonic brain
(Fisher et al., 2012)
embryonic/larval midgut
(Fisher et al., 2012)
embryonic/larval nervous system
(Fisher et al., 2012)
lateral cord
(Fisher et al., 2012)
ventral nerve cord
(Fisher et al., 2012)
northern blot
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage & oogenesis
oocyte
(Newmark and Boswell, 1994)
nurse cell
(Newmark and Boswell, 1994)
embryonic stage -- adult stage
(Newmark and Boswell, 1994)
embryonic stage 1 -- 16
organism
(Newmark and Boswell, 1994)
Additional Descriptive Data
mago transcripts are expressed throughout development and are present in both adult males and females. They are uniformly expressed throughout the nurse cell-oocyte complex during early oogenesis, are abundant in nurse cells at stage 10, and appear to be uniformly distributed throughout the embryo by the time of egg deposition.
(Newmark and Boswell, 1994)
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
epitope tag
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage | female & oogenesis stage S9
nurse cell
• nucleus
(van Eeden et al., 2001)
ring canal | surrounding
(van Eeden et al., 2001)
oocyte | posterior
• nucleus

Comment: tightly localized

(van Eeden et al., 2001)
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
oogenesis & adult stage | female
oocyte
(Parma et al., 2007)
Additional Descriptive Data
mago colocalizes with orb throughout the germarium.
(Parma et al., 2007)
mago protein is localized to the nuclei of nurse cells in the developing egg chamber. mago also colocalizes with btz in a dense cloud around the ring canals, and at the posterior pole of stage 9 oocytes.
(van Eeden et al., 2001)
Marker for
 
Subcellular Localization
CV Term
Evidence
References
catalytic step 2 spliceosome
inferred from high throughput direct assay
(Herold et al., 2009)
cytoplasm
inferred from direct assay
(Parma et al., 2007, Mohr et al., 2001)
exon-exon junction complex
inferred from physical interaction with FLYBASE:tsu; FB:FBgn0033378
(Lewandowski et al., 2010)
nucleus
inferred from direct assay
(Parma et al., 2007, Le Hir et al., 2001, Mohr et al., 2001)
pole plasm
inferred from direct assay
(Palacios et al., 2004)
precatalytic spliceosome
inferred from high throughput direct assay
(Herold et al., 2009)
Expression Deduced from Reporters
Reporter: P{GFPmago17.1}
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage | female & oogenesis stage S9
nurse cell
• nucleus
(van Eeden et al., 2001)
ring canal | surrounding
(van Eeden et al., 2001)
oocyte | posterior
• nucleus

Comment: tightly localized

(van Eeden et al., 2001)
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\mago in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 1-3
  • Stages(s) 11-12
  • Stages(s) 13-16
FlyBase Wiki
Image Based Resources
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 14 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 14 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of mago
Transgenic constructs containing regulatory region of mago
Deletions and Duplications ( 14 )
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal (with Df(2R)AA21)
lethal | female limited, with Scer\GAL4dome-PG14
lethal | maternal effect | recessive | cold sensitive
lethal | recessive
lethal | recessive | heat sensitive
lethal - all die before end of embryonic stage | maternal effect
lethal - all die before end of embryonic stage | maternal effect | recessive | heat sensitive
lethal - all die during P-stage, with Scer\GAL4Mef2.PR
partially lethal - majority die, with Scer\GAL4pnr-MD237
viable
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4tin.CΔ4
Sterility
Allele
female sterile, with Scer\GAL4VP16.otu
female sterile | maternal effect | recessive
male sterile | maternal effect | recessive
sterile
Other Phenotypes
Allele
body color defective, with Scer\GAL4pnr-MD237
short lived | heat sensitive, with Scer\GAL4tin.CΔ4
some die during embryonic stage | maternal effect
some die during embryonic stage | recessive | maternal effect | recessive | heat sensitive
some die during pupal stage, with Scer\GAL4pnr-MD237
visible, with Scer\GAL4pnr-MD237
visible | recessive | heat sensitive
wild-type
Phenotype manifest in
Allele
dorsal appendage
egg
egg | germline clone
egg chamber | germline clone
egg operculum
follicle cell
fusome | germline clone
mesothoracic tergum, with Scer\GAL4pnr-MD237
micropyle
microtubule & egg chamber
microtubule & oocyte
oocyte nucleus
oocyte nucleus | germline clone
posterior aeropyle
Orthologs
Downloads
Download All DIOPT Orthologs
Download All OrthoDB Orthologs
Human Orthologs (via DIOPT v7.1)
Homo sapiens (Human) (2)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\MAGOHB
14 of 15
Yes
Yes
Hsap\MAGOH
13 of 15
No
Yes
 
Model Organism Orthologs (via DIOPT v7.1)
Mus musculus (laboratory mouse) (2)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Magohb
14 of 15
Yes
Yes
Mmus\Magoh
12 of 15
No
Yes
Rattus norvegicus (Norway rat) (2)
Rnor\Magoh
12 of 13
Yes
Yes
Rnor\Magohb
9 of 13
No
Yes
Xenopus tropicalis (Western clawed frog) (1)
Xtro\magoh
12 of 12
Yes
Yes
Danio rerio (Zebrafish) (1)
Drer\magoh
14 of 15
Yes
Yes
Caenorhabditis elegans (Nematode, roundworm) (2)
Cele\mag-1
12 of 15
Yes
Yes
 
 
Cele\mai-1
1 of 15
No
No
Arabidopsis thaliana (thale-cress) (1)
Atha\MAGO
8 of 9
Yes
Yes
Saccharomyces cerevisiae (Brewer's yeast) (0)
No records found.
Schizosaccharomyces pombe (Fission yeast) (1)
Spom\mnh1
12 of 12
Yes
Yes
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG09190HJB )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila melanogaster
fruit fly
Dmel\mago
Drosophila suzukii
Spotted wing Drosophila
Dsuz\DS10_00002753
Drosophila simulans
Dsim\GD11565
Drosophila sechellia
Dsec\GM15806
Drosophila erecta
Dere\GG22089
Drosophila yakuba
Dyak\mago
Drosophila ananassae
Dana\GF11935
Drosophila pseudoobscura pseudoobscura
Dpse\GA21763
Drosophila persimilis
Dper\GL10152
Drosophila willistoni
Dwil\GK20976
Drosophila virilis
Dvir\GJ20538
Drosophila mojavensis
Dmoj\GI20805
Drosophila grimshawi
Dgri\GH20720
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150DGQ )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Mdoa\17008822
Glossina morsitans
Tsetse fly
Gmor\GMOY000684
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_11217
Mayetiola destructor
Hessian fly
Mdes\Mdes009754
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL011544
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL010453
Anopheles darlingi
American malaria mosquito
Adar\ADAC003131
Anopheles gambiae
Malaria mosquito
Agam\AGAP010755
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ002949
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0GBK )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bmor\BGIBMGA003398
Danaus plexippus
Monarch butterfly
Dple\DPOGS208733-PA
Heliconius melpomene
Postman butterfly
Hmel\HMEL015246
Apis florea
Little honeybee
Aflo\5130686980
Apis mellifera
Western honey bee
Amel\mago
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP13157
Bombus terrestris
Buff-tailed bumblebee
Bter\15207627
Linepithema humile
Argentine ant
Lhum\LH10926
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:00214c
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG004040
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_08936
Tribolium castaneum
Red flour beetle
Tcas\mago
Pediculus humanus
Human body louse
Phum\PHUM291670
Rhodnius prolixus
Kissing bug
Rpro\RPRC010985
Cimex lectularius
Bed bug
Clec\CLEC004994
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI000063
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_04496
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0GC9 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Smar\SMAR008803
Ixodes scapularis
Black-legged tick
Isca\ISCW015637
Stegodyphus mimosarum
African social velvet spider
Smim\KFM63953.1
Tetranychus urticae
Two-spotted spider mite
Turt\tetur34g01000
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_299767
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0UKA )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Mn
Ciona intestinalis
Vase tunicate
Cint\ENSCING00000002708
Gallus gallus
Domestic chicken
Ggal\MAGOH
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v7.1)
Drosophila melanogaster (Fruit fly) (0)
No records found.
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    MAGOHB; mago homolog B, exon junction complex subunit
    14 of 15
        MAGOH; mago homolog, exon junction complex subunit
        13 of 15
        602603
             
            Functional Complementation Data
            Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
            Dmel gene
            Ortholog showing functional complementation
            Supporting References
            mago
            Cele\mag-1
            (Newmark et al., 1997)
            Interactions
            Summary of Physical Interactions
            esyN Network Diagram
            Show neighbor-neighbor interactions:
            Select Layout:
            Legend:
            Protein
            RNA
            Selected Interactor(s)
            Interactions Browser
            View in Interactions Browser
            Please see the Physical Interaction reports below for full details
            protein-protein
            Physical Interaction
            Assay
            References
            mago - CG7483
            pull down, molecular weight estimation by staining, anti tag coimmunoprecipitation, western blot, anti bait coimmunoprecipitation
            (Obrdlik et al., 2019, Ghosh et al., 2014, Palacios et al., 2004)
            mago - Pym
            anti tag coimmunoprecipitation, western blot, experimental knowledge based, pull down, molecular weight estimation by staining, x-ray crystallography, anti bait coimmunoprecipitation
            (Ghosh et al., 2014, Guruharsha et al., 2011, Bono et al., 2010, Bono et al., 2004)
            mago - cdm
            pull down, molecular weight estimation by staining, x-ray crystallography
            (Bono et al., 2010)
            mago - ranshi
            anti tag coimmunoprecipitation, western blot
            (Lewandowski et al., 2010)
            mago - tsu
            x-ray crystallography, anti tag coimmunoprecipitation, western blot, anti tag western blot, pull down, molecular weight estimation by staining, molecular sieving, experimental knowledge based
            (Obrdlik et al., 2019, Ghosh et al., 2014, Guruharsha et al., 2011, Bono et al., 2010, Lewandowski et al., 2010, Bono et al., 2004, Fribourg et al., 2003, Shi and Xu, 2003)
            RNA-protein
            Physical Interaction
            Assay
            References
            mago - CG3638
            anti tag coimmunoprecipitation, quantitative reverse transcription pcr
            (Saulière et al., 2010)
            mago - CG7956
            anti tag coimmunoprecipitation, quantitative reverse transcription pcr
            (Saulière et al., 2010)
            mago - bcd
            anti tag coimmunoprecipitation, primer specific pcr
            (Ghosh et al., 2014)
            mago - ftz
            anti tag coimmunoprecipitation, autoradiography
            (Ghosh et al., 2012)
            mago - grk
            anti tag coimmunoprecipitation, primer specific pcr
            (Ghosh et al., 2014)
            mago - nos
            anti tag coimmunoprecipitation, primer specific pcr
            (Ghosh et al., 2014)
            mago - osk
            anti tag coimmunoprecipitation, autoradiography, primer specific pcr
            (Ghosh et al., 2014, Ghosh et al., 2012)
            mago - su(wa)
            anti tag coimmunoprecipitation, quantitative reverse transcription pcr
            (Saulière et al., 2010)
            Summary of Genetic Interactions
            esyN Network Diagram
            esyN Network Key:
            Suppression
            Enhancement
            View in Interactions Browser
            Please look at the allele data for full details of the genetic interactions
            Starting gene(s)
            Interaction type
            Interacting gene(s)
            Reference
            mago
            suppressible
            osk
            (Morais-de-Sá et al., 2013)
            Starting gene(s)
            Interaction type
            Interacting gene(s)
            Reference
            csw
            enhanceable
            mago
            (Ashton-Beaucage et al., 2010)
            Egfr
            suppressible
            mago
            (Ashton-Beaucage et al., 2010)
            Ras85D
            suppressible
            mago
            (Ashton-Beaucage et al., 2010)
            rl
            enhanceable
            mago
            (Ashton-Beaucage et al., 2010)
            Tm1|osk
            enhanceable
            mago
            (Jankovics et al., 2001)
            External Data
            Subunit Structure (UniProtKB)
            Heterodimer with tsu/RBM8A (PubMed:12704080, PubMed:12730685, PubMed:14968132). Part of the mRNA splicing-dependent exon junction complex (EJC) complex; the core complex contains btz/CASC3, eIF4AIII, mago and tsu/RBM8A (PubMed:14973490). Interacts with Pym (via N-terminus); the interaction is direct (PubMed:24967911, PubMed:14968132). Interacts with eIF4AIII (PubMed:14973490).
            (UniProt, P49028 )
            Linkouts
            BioGRID - A database of protein and genetic interactions.
            DroID - A comprehensive database of gene and protein interactions.
            InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
            MIST (genetic) - An integrated Molecular Interaction Database
            MIST (protein-protein) - An integrated Molecular Interaction Database
            Pathways
            Signaling Pathways (FlyBase)
            Metabolic Pathways
            External Data
            Genomic Location and Detailed Mapping Data
            Chromosome (arm)
            2R
            Recombination map
            2-93
            Cytogenetic map
            57C2-57C2
            Sequence location
            2R:21,128,577..21,129,737 [+]
            FlyBase Computed Cytological Location
            Cytogenetic map
            Evidence for location
            57C2-57C2
            Limits computationally determined from genome sequence between P{PZ}king-tubby02821&P{lacW}Xbp1k13803 and P{EP}CG4266EP2258
            Experimentally Determined Cytological Location
            Cytogenetic map
            Notes
            References
            57B20-57C2
            (Newmark, 1993.11.17)
            Experimentally Determined Recombination Data
            Location
            2-93
            (FlyBase, 2016)
            2-100.3
            (Comeron et al., 2012)
            Left of (cM)
            Right of (cM)
            Notes
            Stocks and Reagents
            Stocks (10)
            Genomic Clones (18)
             

            Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

            cDNA Clones (70)
             

            Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

            cDNA clones, fully sequences
            BDGP DGC clones
            Other clones
            Drosophila Genomics Resource Center cDNA clones

            For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

            cDNA Clones, End Sequenced (ESTs)
            BDGP DGC clones
            Other clones
            RNAi and Array Information
            Linkouts
            DRSC - Results frm RNAi screens
            GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
            Antibody Information
            Laboratory Generated Antibodies
             
            polyclonal
            (Choudhury et al., 2016)
            Commercially Available Antibodies
             
            Other Information
            Relationship to Other Genes
            Source for database identify of
            Source for database merge of
            Additional comments
            Other Comments
            mago has a role in the splicing regulation of genes containing long introns.
            (Ashton-Beaucage et al., 2010)
            mago has a role in the splicing regulation of heterochromatic genes containing long introns.
            (Roignant and Treisman, 2010)
            mago is required for female germline stem cell differentiation.
            (Parma et al., 2007)
            dsRNA has been made from templates generated with primers directed against this gene.
            (Roignant et al., 2006)
            dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
            (Kiger et al., 2003)
            The mago/tsu physical interaction is conserved, occurs in vitro and in vivo and is not RNA mediated. mago and tsu gene products localize within nuclear speckles. mago/tsu heterodimers associate with spliced mRNAs carrying an exon-exon junction complex.
            (Le Hir et al., 2001)
            mago and tsu are required for cell viability.
            (Le Hir et al., 2001)
            mago plays two essential roles in Drosophila axis formation: it is required downstream of the signal from the posterior follicle cells for the polarisation of the oocyte microtubule cytoskeleton, and it has a second, independent role in the localisation of osk mRNA to the posterior of the oocyte.
            (Micklem et al., 1997)
            mago protein must be localised within the posterior pole plasm for germ-plasm assembly.
            (Newmark et al., 1997)
            Distribution of tud protein in mutant embryos has been studied.
            (Bardsley et al., 1993)
            mago gene product is a component of the posterior determinative system, required during oogenesis for germ cell determination and delineation of the longitudinal axis of the embryo.
            (Boswell et al., 1991)
            Origin and Etymology
            Discoverer
            Etymology
            "mago nashi" is Japanese for "without grandchildren".
            (Newmark and Boswell, 1994)
            Identification
            External Crossreferences and Linkouts ( 48 )
            Sequence Crossreferences
            NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
            GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
            GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
            RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
            UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
            Other crossreferences
            BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
            Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
            Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
            Flygut - An atlas of the Drosophila adult midgut
            GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
            InterPro - A database of protein families, domains and functional sites
            KEGG Genes - Molecular building blocks of life in the genomic space.
            modMine - A data warehouse for the modENCODE project
            PDB - An information portal to biological macromolecular structures
            Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
            Linkouts
            BioGRID - A database of protein and genetic interactions.
            DPiM - Drosophila Protein interaction map
            DroID - A comprehensive database of gene and protein interactions.
            DRSC - Results frm RNAi screens
            FLIGHT - Cell culture data for RNAi and other high-throughput technologies
            FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
            FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
            FlyMine - An integrated database for Drosophila genomics
            Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
            InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
            KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
            MIST (genetic) - An integrated Molecular Interaction Database
            MIST (protein-protein) - An integrated Molecular Interaction Database
            Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
            Synonyms and Secondary IDs (16)
            Reported As
            Symbol Synonym
            CG9401
            (Meadows, 2018.4.4, Hosono et al., 2015, Ni et al., 2011.7.19, Ni et al., 2010.12.1, Herold et al., 2009, Perkins et al., 2009, Gandhi et al., 2007)
            Dm MGN
            (Le Hir et al., 2001)
            DmMago
            (Palacios, 2002)
            MAGO
            (Choudhury et al., 2016)
            MAGOH
            (Behm-Ansmant and Izaurralde, 2006)
            MGN
            (Le Hir et al., 2001)
            Mago
            (Obrdlik et al., 2019, Gáspár et al., 2017, Le Thomas et al., 2013, Ghosh et al., 2012, Bono et al., 2010, Lewandowski et al., 2010, Saulière et al., 2010, Minakhina and Steward, 2005, Bono et al., 2004, Fribourg et al., 2003)
            Magoh
            (Le Hir et al., 2003)
            l(2)57Ca
            (O'Donnell et al., 1989)
            mago
            (Gao et al., 2019, Trcek and Lehmann, 2019, Whittle and Extavour, 2019, Wang et al., 2018, Ashton-Beaucage and Therrien, 2017, Garcia-Garcia et al., 2017, Transgenic RNAi Project members, 2017-, Ashton-Beaucage et al., 2016, Dennis et al., 2016, Malartre, 2016, Sarov et al., 2016, Yan and Perrimon, 2015, Ashton-Beaucage et al., 2014, Ghosh et al., 2014, Hayashi et al., 2014, Czech et al., 2013, Handler et al., 2013, Handler et al., 2013, Morais-de-Sá et al., 2013, Treisman, 2013.10.7, Ashton-Beaucage and Therrien, 2011, Friedman et al., 2011, Ashton-Beaucage et al., 2010, Roignant and Treisman, 2010, Baker et al., 2009, Firth and Baker, 2009, Herold et al., 2009, Tuxworth et al., 2009, Okamura et al., 2008, Zimyanin et al., 2008, Roignant et al., 2007, Zimyanin et al., 2007, Doerflinger et al., 2006, Irion et al., 2006, Wang et al., 1994)
            mgn
            (Foe et al., 1993, Newmark, 1993.11.17, Lindsley and Zimm, 1986)
            Name Synonyms
            Mago Nashi
            (Lewandowski et al., 2010, Nie et al., 2009, Parma et al., 2007)
            Mago nashi
            (Zimyanin et al., 2008, Hachet and Ephrussi, 2004)
            mago nashi
            (Jakšić and Schlötterer, 2016, Malartre, 2016, Handler et al., 2013, Roignant and Treisman, 2010, Tuxworth et al., 2009)
            mago nishi
            (Roignant et al., 2006)
            mago-nashi
            (Tuxworth et al., 2009, Hachet and Ephrussi, 2002)
            Secondary FlyBase IDs
            • FBgn0002095
            Datasets (0)
            Study focus (0)
            Experimental Role
            Project
            Project Type
            Title
            References (167)