RLC, MRLC, Dmlc2, myosin regulatory light chain, Ifm(3)99Eb
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.49
There is only one protein coding transcript and one polypeptide associated with this gene
Myosin is a hexamer of 2 heavy chains and 4 light chains.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Mlc2 using the Feature Mapper tool.
GBrowse - Visual display of RNA-Seq signalsView Dmel\Mlc2 in GBrowse 2
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: Mlc2 CG2184
Haploinsufficient locus (not associated with strong haplolethality or haplosterility).
Identified as a potential component of the hh signalling pathway in a genome-wide RNAi screen. dsRNA made from templates generated with primers directed affects the extent of expression of a hh signaling reporter construct in Clone 8 cells.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
Analysis of Mlc2 mutants in which one or both Strn-Mlck phosphorylation sites have been mutated suggests that a decrease in the constitutive level of phosphorylation of the Mlc2 protein, without detectable disruption of sarcomere structure or cross-bridge derangement, decreases stretch activation and net power output of the indirect flight muscle by reducing the number of attached cross-bridges rather than by changing the kinetics of the power-producing step of the attached cross-bridges.
Phosphorylation of indirect fight muscle is not required for calcium-activated force obtained at low strain but the absence of phosphorylation severely reduces the power output in the oscillating muscle which depends upon stretch activation.
Strn-Mlck-dependent phosphorylation of Mlc2 is not required for myofibrillogenesis or for the development of maximal isometric force in indirect flight muscles. Single or double substitutions at the major phosphorylation site produce reduced power output in isolated flight muscle fibres and reduced flight ability showing that myosin regulatory light chain phosphorylation is a key determinant of the stretch activation response.
Tm1, muscle promoter of Tm2, Act57B and Mlc2 were not transcribed by embryonic extracts. The contractile protein genes may possess a common feature related to in vitro transcription that prevents their transcription.
Isolated from a genomic library using a probe enriched for sequences complementary to myotube-specific mRNA.