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FB2021_03, released June 15, 2021

Gene: Dmel\Rpn8

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General Information
Symbol
Dmel\Rpn8
Species
D. melanogaster
Name
Regulatory particle non-ATPase 8
Annotation Symbol
CG3416
Feature Type
protein_coding_gene
FlyBase ID
FBgn0002787
Gene Model Status
Current
Stock Availability
12 publicly available
Gene Summary
Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins. (UniProt, P26270)
Contribute a Gene Snapshot for this gene.
All Summaries
Also Known As

Mov34, p39B, l(2)k08003

Key Links
Genomic Location
Cytogenetic map
60C8-60C8
Sequence location
2R:24,400,028..24,404,527 [+]
Recombination map
2-106
RefSeq locus
NT_033778 REGION:24400028..24404527
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (7 terms)
Molecular Function (2 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
enables isopeptidase activity
inferred from electronic annotation with InterPro:IPR000555
(assigned by InterPro )
(FlyBase Curators et al., 2004-)
NOT enables metallopeptidase activity
inferred from sequence or structural similarity with UniProtKB:P51665
(FlyBase Curators, 2008--)
Biological Process (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process
inferred from mutant phenotype
(Lőw et al., 2013)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process
inferred from biological aspect of ancestor with PANTHER:PTN000057040
(assigned by GO_Central )
(Gaudet et al., 2011)
inferred by curator from GO:0005838
(Sumegi et al., 2003)
Cellular Component (4 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
located_in dendrite
inferred from mutant phenotype
(Kuo et al., 2005)
part_of proteasome complex
inferred from direct assay
(Nickell et al., 2009)
part_of proteasome regulatory particle
inferred from direct assay
(Sumegi et al., 2003, Holzl et al., 2000)
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
part_of proteasome complex
inferred from biological aspect of ancestor with PANTHER:PTN000057040
(assigned by GO_Central )
(Gaudet et al., 2011)
part_of proteasome regulatory particle, lid subcomplex
inferred from sequence or structural similarity with SGD:S000005787
(Holzl et al., 2000)
part_of proteasome regulatory particle
inferred from sequence or structural similarity with HGNC:9565
(Holzl et al., 2000)
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the peptidase M67A family. (P26270)
Protein Signatures (InterPro)
Summaries
Gene Group (FlyBase)
M67 NON-PEPTIDASE HOMOLOGS -
M67 non-peptidase homologs are similar in amino acid sequence to JAMM deubiquitinases (M67 metallopeptidases) but lack one or more of the expected catalytic residues or zinc-binding sites. They may act as scaffold proteins or as ubiquitin-binding modules within complexes. (Adapted from FBrf0239469 and PMID:17559875.)
PROTEASOME REGULATORY PARTICLE NON-ATPASES -
The 26S proteasome degrades polyubiquitinated proteins in the cytoplasm and nucleus. It is formed from a cylindrical catalytic core 20S proteasome capped at each end by a regulatory 19S particle. The 19S regulatory particle recognizes, unfolds, and translocates ubiquitinated proteins into the catalytic core particle in an ATP-dependent manner. 'Rpn' subunits are non-ATPase subunits located in the base and lid subcomplexes of the regulatory particle (Adapted from FBrf0215459 and PMID:28583440.)
Protein Function (UniProtKB)
Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.
(UniProt, P26270)
Gene Model and Products
Number of Transcripts
3
Number of Unique Polypeptides
2

Please see the JBrowse view of Dmel\Rpn8 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Gene model reviewed during 5.44

Stop-codon suppression (UAG) postulated; FBrf0216884

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.46

Sequence Ontology: Class of Gene
gene_with_stop_codon_read_through
(Jungreis et al., 2011)
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Rpn8-RA
FBtr0072290
NM_079121
1456
338
Rpn8-RB
FBtr0330237
NM_001274238
1456
358
Rpn8-RC
FBtr0332921
NM_001274239
1473
338
Additional Transcript Data and Comments
Reported size (kB)

1.4 (northern blot)

(Gridley et al., 1990)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
Rpn8-PA
FBpp0072197
38.1
338
6.69
NP_523845
AAF47199
Rpn8-PB
FBpp0303270
40.6
358
9.55
NP_001261167
AGB93697
Rpn8-PC
FBpp0305141
38.1
338
6.69
NP_001261168
AGB93698
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

338 aa isoforms: Rpn8-PA, Rpn8-PC
Additional Polypeptide Data and Comments
Reported size (kDa)

338 (aa)

(Gridley et al., 1990)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Nucleotide / Polypeptide Records
 
UniProt
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Rpn8 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 1 -- 3
pole plasm

Comment: maternally deposited

(Fisher et al., 2012)
organism

Comment: maternally deposited

(Fisher et al., 2012)
embryonic stage 4 -- 6
organism | ubiquitous
(Fisher et al., 2012)
primordial germ cell
(Fisher et al., 2012)
embryonic stage 7 -- 8
anterior endoderm anlage
(Fisher et al., 2012)
head mesoderm
(Fisher et al., 2012)
organism | ubiquitous
(Fisher et al., 2012)
primordial germ cell
(Fisher et al., 2012)
posterior endoderm
(Fisher et al., 2012)
trunk mesoderm
(Fisher et al., 2012)
embryonic stage 9 -- 10
anterior endoderm
(Fisher et al., 2012)
head mesoderm
(Fisher et al., 2012)
organism | ubiquitous
(Fisher et al., 2012)
posterior endoderm
(Fisher et al., 2012)
trunk mesoderm
(Fisher et al., 2012)
embryonic stage 11 -- 12
anterior midgut primordium
(Fisher et al., 2012)
central brain primordium
(Fisher et al., 2012)
head mesoderm
(Fisher et al., 2012)
organism | ubiquitous
(Fisher et al., 2012)
posterior midgut primordium
(Fisher et al., 2012)
trunk mesoderm
(Fisher et al., 2012)
ventral nerve cord primordium
(Fisher et al., 2012)
embryonic stage 13 -- 16
embryonic brain
(Fisher et al., 2012)
embryonic/larval midgut
(Fisher et al., 2012)
embryonic/larval nervous system
(Fisher et al., 2012)
gonad
(Fisher et al., 2012)
lateral cord neuron
(Fisher et al., 2012)
organism | faint | ubiquitous
(Fisher et al., 2012)
organism | ubiquitous
(Fisher et al., 2012)
larval ventral nerve cord
(Fisher et al., 2012)
northern blot
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
(Gridley et al., 1990)
Additional Descriptive Data

The 1.4 kb transcript is detected in adult heads and bodies.

(Gridley et al., 1990)
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Aradska et al., 2015)
day 7 of adulthood -- day 49 of adulthood
adult heart
(Cammarato et al., 2011)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in dendrite
inferred from mutant phenotype
(Kuo et al., 2005)
part_of proteasome complex
inferred from direct assay
(Nickell et al., 2009)
part_of proteasome regulatory particle
inferred from direct assay
(Sumegi et al., 2003, Holzl et al., 2000)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Rpn8 in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB
EMBL-EBI Single Cell Expression Atlas
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 4-6
  • Stages(s) 7-8
  • Stages(s) 9-10
  • Stages(s) 11-12
  • Stages(s) 13-16
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 5 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 11 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Rpn8
Transgenic constructs containing regulatory region of Rpn8
Aberrations (Deficiencies and Duplications) ( 6 )
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal
lethal, with Scer\GAL4GMR71G10, Spyo\Cas9UAS.P2
lethal, with Scer\GAL4GMR71G10, Spyo\Cas9UAS.Tag:NLS(Unk)
lethal, with Scer\GAL4pnr-MD237
lethal, with Spyo\Cas9UAS.Tag:NLS(Unk)
lethal (with Df(2R)BSC603)
lethal (with Df(2R)ED4061)
lethal | larval stage, with Scer\GAL4sqh.PW
lethal | pharate adult stage, with Scer\GAL4elav.PU
lethal | pupal stage, with Scer\GAL4elav.PU
lethal | pupal stage, with Scer\GAL4sqh.PW
lethal | recessive
lethal - all die before end of pupal stage, with Scer\GAL4dpp.PU
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4GMR71G10, Spyo\Cas9UAS.P2
Other Phenotypes
Allele
abnormal neuroanatomy | adult stage, with Scer\GAL4GMR71G10
abnormal neuroanatomy | adult stage, with Scer\GAL4GMR71G10, Spyo\Cas9UAS.P2
abnormal neuroanatomy | larval stage
abnormal neuroanatomy | pupal stage
abnormal neuroanatomy | somatic clone
decreased cell number | somatic clone
decreased fecundity | female, with Scer\GAL4VP16.mat.αTub67C
some die during pupal stage, with Scer\GAL4dpp.PU
visible, with Scer\GAL4bbg-C96
Phenotype manifest in
Allele
actin cytoskeleton | adult stage | female, with Scer\GAL4VP16.mat.αTub67C
adult mushroom body | somatic clone
adult mushroom body gamma-lobe | somatic clone
axon | adult stage, with Scer\GAL4GMR71G10
axon | adult stage, with Scer\GAL4GMR71G10, Spyo\Cas9UAS.P2
dendritic arborizing neuron | P-stage
egg chamber, with Scer\GAL4VP16.mat.αTub67C
embryonic/larval fat body | third instar larval stage, with Scer\GAL4Cg.PA
embryonic/larval hemocyte | third instar larval stage, with Scer\GAL4Pxn.PS
gamma Kenyon cell | adult stage, with Scer\GAL4GMR71G10
gamma Kenyon cell | adult stage, with Scer\GAL4GMR71G10, Spyo\Cas9UAS.P2
ganglion mother cell | larval stage
larval neuroblast | larval stage
mitochondrion | third instar larval stage, with Scer\GAL4Cg.PA
neuroblast | somatic clone
nurse cell ring canal, with Scer\GAL4VP16.mat.αTub67C
wing, with Scer\GAL4bbg-C96
Orthologs
Downloads
Download All DIOPT Orthologs
Download All OrthoDB Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (10)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\PSMD7
14 of 15
Yes
Yes
Hsap\BRCC3
1 of 15
No
Yes
Hsap\COPS5
1 of 15
No
No
1  
Hsap\COPS6
1 of 15
No
No
Hsap\EIF3F
1 of 15
No
No
Hsap\MPND
1 of 15
No
No
Hsap\MYSM1
1 of 15
No
No
Hsap\PSMD14
1 of 15
No
No
Hsap\STAMBP
1 of 15
No
No
1  
Hsap\STAMBPL1
1 of 15
No
No
1  
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (10)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Psmd7
14 of 15
Yes
Yes
Mmus\Brcc3
1 of 15
No
Yes
Mmus\Cops5
1 of 15
No
No
Mmus\Cops6
1 of 15
No
No
Mmus\Eif3f
1 of 15
No
No
Mmus\Mpnd
1 of 15
No
No
Mmus\Mysm1
1 of 15
No
No
Mmus\Psmd14
1 of 15
No
No
Mmus\Stambpl1
1 of 15
No
No
Mmus\Stambp
1 of 15
No
No
Rattus norvegicus (Norway rat) (9)
Rnor\Psmd7
12 of 13
Yes
Yes
Rnor\Eif3f
2 of 13
No
No
Rnor\Brcc3
1 of 13
No
Yes
Rnor\Cops5
1 of 13
No
No
Rnor\Cops6
1 of 13
No
No
Rnor\Mpnd
1 of 13
No
No
Rnor\Mysm1
1 of 13
No
No
Rnor\Stambpl1
1 of 13
No
No
Rnor\Stambp
1 of 13
No
No
Xenopus tropicalis (Western clawed frog) (10)
Xtro\psmd7
12 of 12
Yes
Yes
Xtro\eif3f
2 of 12
No
No
Xtro\brcc3
1 of 12
No
Yes
Xtro\cops5
1 of 12
No
No
Xtro\cops6
1 of 12
No
No
Xtro\mpnd
1 of 12
No
No
Xtro\mysm1
1 of 12
No
No
Xtro\psmd14
1 of 12
No
No
Xtro\stambpl1
1 of 12
No
No
Xtro\stambp
1 of 12
No
No
Danio rerio (Zebrafish) (10)
Drer\psmd7
11 of 15
Yes
Yes
Drer\eif3f
2 of 15
No
No
Drer\brcc3
1 of 15
No
Yes
Drer\cops5
1 of 15
No
No
Drer\mpnd
1 of 15
No
No
Drer\mysm1
1 of 15
No
No
Drer\psmd14
1 of 15
No
No
Drer\stambpa
1 of 15
No
No
Drer\stambpb
1 of 15
No
No
Drer\stambpl1
1 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (5)
Cele\rpn-8
14 of 15
Yes
Yes
Cele\eif-3.F
2 of 15
No
No
Cele\csn-5
1 of 15
No
No
Cele\csn-6
1 of 15
No
No
Cele\rpn-11
1 of 15
No
No
Arabidopsis thaliana (thale-cress) (2)
Atha\MEE34
9 of 9
Yes
Yes
Atha\RPN8A
9 of 9
Yes
Yes
Saccharomyces cerevisiae (Brewer's yeast) (3)
Scer\RPN8
13 of 15
Yes
Yes
Scer\RPN11
1 of 15
No
No
Scer\RRI1
1 of 15
No
No
Schizosaccharomyces pombe (Fission yeast) (1)
Spom\rpn8
10 of 12
Yes
Yes
Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG09190CNL )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Dsuz\DS10_00002251
Drosophila simulans
Dsim\GD11854
Drosophila sechellia
Dsec\GM11856
Drosophila erecta
Dere\GG22963
Drosophila yakuba
Dyak\GE14400
Drosophila ananassae
Dana\GF13608
Drosophila pseudoobscura pseudoobscura
Dpse\GA17437
Drosophila persimilis
Dper\GL20068
Drosophila willistoni
Dwil\GK22207
Drosophila virilis
Dvir\GJ21597
Drosophila mojavensis
Dmoj\GI21325
Drosophila grimshawi
Dgri\GH20991
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG0915089I )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Mdoa\17027521
Glossina morsitans
Tsetse fly
Gmor\GMOY005744
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_03221
Mayetiola destructor
Hessian fly
Mdes\Mdes011268
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL000270
Anopheles darlingi
American malaria mosquito
Adar\ADAC002522
Anopheles gambiae
Malaria mosquito
Agam\AGAP012135
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ015055
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0A69 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bmor\BGIBMGA005994
Danaus plexippus
Monarch butterfly
Dple\DPOGS214282-PA
Apis florea
Little honeybee
Aflo\3646977028
Apis mellifera
Western honey bee
Amel\LOC408413
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP23615
Bombus terrestris
Buff-tailed bumblebee
Bter\15210220
Linepithema humile
Argentine ant
Lhum\LH16556
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:001797
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG001600
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_07278
Tribolium castaneum
Red flour beetle
Tcas\TC013318
Pediculus humanus
Human body louse
Phum\PHUM077900
Rhodnius prolixus
Kissing bug
Rpro\RPRC007726
Cimex lectularius
Bed bug
Clec\CLEC006425
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI007679
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_09551
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_09553
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X08WI )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Smar\SMAR015103
Ixodes scapularis
Black-legged tick
Isca\ISCW022113
Stegodyphus mimosarum
African social velvet spider
Smim\KFM67634.1
Tetranychus urticae
Two-spotted spider mite
Turt\tetur26g02250
Tetranychus urticae
Two-spotted spider mite
Turt\tetur469g00010
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_49603
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0DX6 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Psmd7Lp26SnATPase7
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Psmd7
Ciona intestinalis
Vase tunicate
Cint\LOC100183181
Gallus gallus
Domestic chicken
Ggal\PSMD7
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (7)
Gene Symbol
Score
Source
Compara
eggNOG
Homologene
Inparanoid
Isobase
OrthoDB
OrthoFinder
orthoMCL
Panther
RoundUp
Alignment
eIF3f1
4 of 10
eIF3f2
4 of 10
CSN6
3 of 10
CG2224
1 of 10
CG4751
1 of 10
CSN5
1 of 10
Rpn11
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    PSMD7; proteasome 26S subunit, non-ATPase 7
    14 of 15
    157970
        Functional Complementation Data
        Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
        Interactions
        Summary of Physical Interactions
        esyN Network Diagram
        Show neighbor-neighbor interactions:
        Select Layout:
        Legend:
        Protein
        RNA
        Selected Interactor(s)
        Interactions Browser
        View in Interactions Browser
        Please see the Physical Interaction reports below for full details
        protein-protein
        Physical Interaction
        Assay
        References
        Rpn8 - Prosα2
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpn8 - Prosα3
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpn8 - Prosα4
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpn8 - Prosα5
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpn8 - Prosα6
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpn8 - Prosα7
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpn8 - Prosβ1
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpn8 - Prosβ2
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpn8 - Prosβ3
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpn8 - Prosβ4
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpn8 - Prosβ5
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpn8 - Prosβ6
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpn8 - Prosβ7
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpn8 - Rpn1
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpn8 - Rpn11
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpn8 - Rpn13
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpn8 - Rpn2
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpn8 - Rpn3
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpn8 - Rpn5
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpn8 - Rpn6
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpn8 - Rpn7
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpn8 - Rpn9
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpn8 - Rpt2
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpn8 - Rpt3
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpn8 - Rpt4
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpn8 - Rpt5
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpn8 - Rpt6
        experimental knowledge based
        (Guruharsha et al., 2011)
        Summary of Genetic Interactions
        esyN Network Diagram
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        External Data
        Linkouts
        BioGRID - A database of protein and genetic interactions.
        DroID - A comprehensive database of gene and protein interactions.
        InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
        MIST (protein-protein) - An integrated Molecular Interaction Database
        Pathways
        Signaling Pathways (FlyBase)
        Metabolic Pathways
        External Data
        Linkouts
        KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
        Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
        Genomic Location and Detailed Mapping Data
        Chromosome (arm)
        2R
        Recombination map
        2-106
        Cytogenetic map
        60C8-60C8
        Sequence location
        2R:24,400,028..24,404,527 [+]
        FlyBase Computed Cytological Location
        Cytogenetic map
        Evidence for location
        60C8-60C8
        Limits computationally determined from genome sequence between P{PZ}bs03267&P{lacW}l(2)k10502k10502 and P{lacW}Nurf-38k16102
        Experimentally Determined Cytological Location
        Cytogenetic map
        Notes
        References
        60D1-60D2
        (determined by in situ hybridisation)
        (Spradling et al., 1999)
        60D1-60D2
        (Beaton, 1997.12.27, BDGP Project Members, 1994-1999)
        60B-60C
        (determined by in situ hybridisation)
        (Gridley et al., 1990)
        Experimentally Determined Recombination Data
        Location

        2-106

        (FlyBase, 2016)

        2-110.6

        (Comeron et al., 2012)
        Left of (cM)
        Right of (cM)
        Notes
        Stocks and Reagents
        Stocks (12)
        Genomic Clones (16)
         

        Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

        cDNA Clones (193)
         

        Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

        cDNA clones, fully sequenced
        BDGP DGC clones
        Other clones
        Drosophila Genomics Resource Center cDNA clones

        For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

        cDNA Clones, End Sequenced (ESTs)
        BDGP DGC clones
        Other clones
        RNAi and Array Information
        Linkouts
        DRSC - Results frm RNAi screens
        GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
        Antibody Information
        Laboratory Generated Antibodies
         
        Commercially Available Antibodies
         
        Other Information
        Relationship to Other Genes
        Source for database identify of

        Source for identity of: Mov34 CG3416

        (Wang, 1999.11)

        Source for identity of: Rpn8 Mov34

        (FlyBase, 2009-)
        Source for database merge of

        Source for merge of: Mov34 l(2)k08003

        (Spradling et al., 1999)
        Additional comments

        The nomenclature of genes encoding subunits of the 26S proteasome of D. melanogaster have been standardized according to FBrf0215459. These symbols/names largely follow those used already in FlyBase, and largely mirror fly community usage. HOWEVER, note that at least one other nomenclature system exists that is followed by the HUGO Gene Nomenclature Committee (HGNC), for example, with the unfortunate result that several D. melanogaster genes have shared synonyms.

        (FlyBase, 2009-)
        Other Comments

        RNAi screen using dsRNA made from templates generated with primers directed against this gene results in the formation of short, monastral bipolar spindles and severe proliferation defects when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.

        (Goshima et al., 2007)

        RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R+ cells.

        (Boutros et al., 2004)

        RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R+ cells.

        (Boutros et al., 2004)

        The 26S proteolytic complex, a multiprotein complex containing many subunits (including those encoded by Pros26.4, Pros54 and Mov34), can be assembled from two smaller multiprotein complexes, the μ particle and the 20S proteasome, by a process that requires ATP.

        (Udvardy, 1993)

        Mov34 has been cloned and analysed.

        (Gridley et al., 1990)
        Origin and Etymology
        Discoverer
        Etymology
        Identification
        External Crossreferences and Linkouts ( 92 )
        Sequence Crossreferences
        NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
        GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
        GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
        RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
        UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
        UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
        Other crossreferences
        BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
        Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
        DRscDB
        EMBL-EBI Single Cell Expression Atlas
        Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
        Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
        Flygut - An atlas of the Drosophila adult midgut
        Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
        GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
        InterPro - A database of protein families, domains and functional sites
        KEGG Genes - Molecular building blocks of life in the genomic space.
        MARRVEL_MODEL
        modMine - A data warehouse for the modENCODE project
        Linkouts
        BioGRID - A database of protein and genetic interactions.
        DroID - A comprehensive database of gene and protein interactions.
        DRSC - Results frm RNAi screens
        FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
        FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
        FlyMine - An integrated database for Drosophila genomics
        InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
        KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
        MIST (protein-protein) - An integrated Molecular Interaction Database
        Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
        Synonyms and Secondary IDs (11)
        Reported As
        Symbol Synonym
        CG3416
        (Kolkhof et al., 2017, Çiçek et al., 2016, Rosa-Ferreira et al., 2015, Tsou et al., 2015, Wang et al., 2014, Schertel et al., 2013, Yoshigi et al., 2013, Tsou et al., 2012, Zhang et al., 2012, Zhang et al., 2012, Cammarato et al., 2011, Ni et al., 2011.7.19, Belote and Zhong, 2009, Keleman et al., 2009.8.5, Lebo et al., 2009, Perkins et al., 2009, Ni et al., 2008, Barbash and Lorigan, 2007, Gandhi et al., 2007, Goshima et al., 2007, Molnar et al., 2006, Lundgren et al., 2005, Nakahara et al., 2005, Ma et al., 2002)
        Mov34
        (Meltzer et al., 2019, Herzmann et al., 2017, Lee et al., 2016, Rumpf et al., 2014, de Celis et al., 2013.9.11, Kwon et al., 2013, Lőw et al., 2013, Schertel et al., 2013, Yoshigi et al., 2013, Murray et al., 2012, Tsou et al., 2012, Valakh et al., 2012, Zhang et al., 2012, Zhang et al., 2012, Du et al., 2011, Jungreis et al., 2011, Neumüller et al., 2011, Christensen et al., 2009.5.6, Lebo et al., 2009, Christensen et al., 2008.9.3, Hoopfer et al., 2008, Stofanko et al., 2008, Barbash and Lorigan, 2007, Goshima et al., 2007, Stuart et al., 2007, Molnar et al., 2006, Lundgren et al., 2005, Ma et al., 2002)
        P26270
        (Clague et al., 2013)
        PRSC_DROME
        (Aravind and Ponting, 1998)
        PSMD7
        (Tsou et al., 2015)
        RPN8
        (Gemmill et al., 2005)
        Rpn8
        (Fernández-Cruz et al., 2020, Walls et al., 2020, Wei et al., 2020, Kurshakova et al., 2019, Meltzer et al., 2019, Zhou et al., 2019, Gene Disruption Project members, 2018-, Cook, 2015.12.23, Hudson et al., 2015, Wang et al., 2014, Pathare et al., 2012, Lee et al., 2010, Belote and Zhong, 2009, Nickell et al., 2009, Lundgren et al., 2005, Ma et al., 2002)
        S12
        (Lundgren et al., 2005)
        l(2)k08003
        (Beaton, 1997.12.27, BDGP Project Members, 1994-1999)
        p39B
        (Sumegi et al., 2003, Kurucz et al., 2002, Ma et al., 2002, Holzl et al., 2000)
        Name Synonyms
        Mov34
        (Cammarato et al., 2011)
        Regulatory particle non-ATPase 8
        Secondary FlyBase IDs
        • FBgn0022065
        Datasets (0)
        Study focus (0)
        Experimental Role
        Project
        Project Type
        Title
        References (109)