FlyBase Gene Report: Dmel\Rpn8

JAMM DOMAIN-CONTAINING PROTEASES (DEUBIQUITINASES) -: The JAMM (JAB1/MPN/MOV34) sub-family of deubiquitinases are Zn²⁺-binding metalloproteases. In general, JAMMs are components of larger assemblies such as the proteasome and COP9 signalosome. Several members of this family lack the ability to co-ordinate Zn²⁺ and therefore enzymatically inactive; they may act as scaffold proteins or as ubiquitin-binding modules within complexes. (Adapted from PMID:23899565 and PMID:17559875).
PROTEASOME SUBUNITS -: The 26S proteasome degrades polyubiquitinated proteins in the cytoplasm and nucleus. It is formed from a cylindrical catalytic core 20S proteasome capped at each end by a regulatory 19S particle. The 19S regulatory particle recognizes, unfolds, and translocates ubiquitinated proteins into the catalytic core particle in an ATP-dependent manner. (Adapted from FBrf0215459).

Protein Function (UniProtKB)

Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.

(UniProt, P26270)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the GBrowse view of Dmel\Rpn8 or the JBrowse view of Dmel\Rpn8 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.44

Stop-codon suppression (UAG) postulated; FBrf0216884

gene_with_stop_codon_read_through ; SO:0000697

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.46

Sequence Ontology: Class of Gene

gene_with_stop_codon_read_through

(Jungreis et al., 2011)

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Rpn8-RA

FBtr0072290

NM_079121

1456

338

Rpn8-RB

FBtr0330237

NM_001274238

1456

358

Rpn8-RC

FBtr0332921

NM_001274239

1473

338

Additional Transcript Data and Comments

Reported size (kB)

1.4 (northern blot)

(Gridley et al., 1990)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

Rpn8-PA

FBpp0072197

38.1

338

6.69

NP_523845

AAF47199

Rpn8-PB

FBpp0303270

40.6

358

9.55

NP_001261167

AGB93697

Rpn8-PC

FBpp0305141

38.1

338

6.69

NP_001261168

AGB93698

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

338 aa isoforms: Rpn8-PA, Rpn8-PC

Additional Polypeptide Data and Comments

Reported size (kDa)

338 (aa)

(Gridley et al., 1990)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Rpn8 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Gene Ontology (5 terms)

Molecular Function (0 terms)

There is no information supporting a Molecular Function term for this gene. (Based on a review of available data on: 2014-11-21 )

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

proteasome-mediated ubiquitin-dependent protein catabolic process

inferred from mutant phenotype

(Lőw et al., 2013)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

proteasome-mediated ubiquitin-dependent protein catabolic process

inferred by curator from GO:0005838

(Sumegi et al., 2003)

inferred from biological aspect of ancestor with PANTHER:PTN000057040

(assigned by GO_Central )

(Gaudet et al., 2011)

Cellular Component (4 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

dendrite

inferred from mutant phenotype

(Kuo et al., 2005)

proteasome complex

inferred from direct assay

(Nickell et al., 2009)

proteasome regulatory particle

inferred from direct assay

(Sumegi et al., 2003, Holzl et al., 2000)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

proteasome complex

inferred from biological aspect of ancestor with PANTHER:PTN000057040

(assigned by GO_Central )

(Gaudet et al., 2011)

proteasome regulatory particle

inferred from sequence or structural similarity with HGNC:9565

(Holzl et al., 2000)

proteasome regulatory particle, lid subcomplex

inferred from sequence or structural similarity with SGD:S000005787

(Holzl et al., 2000)

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

pole plasm

Comment: maternally deposited

(Fisher et al., 2012)

organism

• pole plasm

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

pole cell

(Fisher et al., 2012)

embryonic stage 7 -- 8

anterior endoderm anlage

(Fisher et al., 2012)

head mesoderm

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

pole cell

(Fisher et al., 2012)

posterior endoderm

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

embryonic stage 9 -- 10

anterior endoderm

(Fisher et al., 2012)

head mesoderm

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

posterior endoderm

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

embryonic stage 11 -- 12

anterior midgut primordium

(Fisher et al., 2012)

central brain primordium

(Fisher et al., 2012)

head mesoderm

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

posterior midgut primordium

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

ventral nerve cord primordium

(Fisher et al., 2012)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

(Gridley et al., 1990)

Additional Descriptive Data

The 1.4 kb transcript is detected in adult heads and bodies.

(Gridley et al., 1990)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

dendrite

inferred from mutant phenotype

(Kuo et al., 2005)

proteasome complex

inferred from direct assay

(Nickell et al., 2009)

proteasome regulatory particle

inferred from direct assay

(Sumegi et al., 2003, Holzl et al., 2000)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Rpn8 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Rpn8

View exonic expression by tissue for Dmel\Rpn8

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0002787

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0002787

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG3416-RA

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG3416

Flygut - An atlas of the Drosophila adult midgut

FBgn0002787

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\Rpn8

Stages(s) 4-6
Stages(s) 7-8
Stages(s) 9-10
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, and Transgenic Constructs

Classical and Insertion Alleles ( 5 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 10 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Rpn8

Transgenic constructs containing regulatory region of Rpn8

Deletions and Duplications ( 6 )

Disrupted in

Df(2R)BSC603

(Kahsai et al., 2016, Christensen et al., 2008.9.3)

Df(2R)BSC780

(Christensen et al., 2009.5.6)

Df(2R)bw^VDe2LPx^KR

(Spradling et al., 1999, Beaton, 1997.12.27)

Df(2R)ED4061

(Kahsai et al., 2016)

Df(2R)Exel6278

(Ryder, 2004.4.26)

In(2LR)Px⁴

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal

Rpn8^GD10960

lethal, with Scer\GAL4^GMR71G10, Spyo\Cas9^UAS.P2

lethal, with Scer\GAL4^GMR71G10, Spyo\Cas9^{UAS.Tag:NLS(Unk)}

lethal, with Scer\GAL4^pnr-MD237

Rpn8^GD10960

lethal, with Spyo\Cas9^{UAS.Tag:NLS(Unk)}

lethal (with Df(2R)BSC603)

Rpn8^k08003

lethal (with Df(2R)ED4061)

Rpn8^k08003

lethal | larval stage, with Scer\GAL4^sqh.PW

Rpn8^KK101319

lethal | pharate adult stage, with Scer\GAL4^elav.PU

Rpn8^KK101319

lethal | pupal stage, with Scer\GAL4^elav.PU

Rpn8^KK101319

lethal | pupal stage, with Scer\GAL4^sqh.PW

Rpn8^KK101319

lethal | recessive

lethal - all die before end of pupal stage, with Scer\GAL4^dpp.PU

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Rpn8^GD10960

viable, with Scer\GAL4^GMR71G10, Spyo\Cas9^UAS.P2

Rpn8^gRNA.pCFD3-1

Other Phenotypes

Allele

decreased cell number | somatic clone

Rpn8^k08003

neuroanatomy defective | adult stage, with Scer\GAL4^GMR71G10

neuroanatomy defective | adult stage, with Scer\GAL4^GMR71G10, Spyo\Cas9^UAS.P2

Rpn8^gRNA.pCFD3-1

neuroanatomy defective | larval stage

Rpn8^GD10960

neuroanatomy defective | pupal stage

Rpn8^k08003

neuroanatomy defective | somatic clone

Rpn8^k08003

some die during pupal stage, with Scer\GAL4^dpp.PU

visible, with Scer\GAL4^bbg-C96

Phenotype manifest in

Allele

actin cytoskeleton | adult stage | female, with Scer\GAL4^{VP16.mat.αTub67C}

Rpn8^GL00333

adult mushroom body | somatic clone

Rpn8^k08003

axon | adult stage, with Scer\GAL4^GMR71G10

axon | adult stage, with Scer\GAL4^GMR71G10, Spyo\Cas9^UAS.P2

Rpn8^gRNA.pCFD3-1

dendritic arborizing neuron | P-stage

Rpn8^k08003

egg chamber, with Scer\GAL4^{VP16.mat.αTub67C}

Rpn8^GL00333

embryonic/larval fat body | third instar larval stage, with Scer\GAL4^Cg.PA

Rpn8^JF01140

embryonic/larval hemocyte | third instar larval stage, with Scer\GAL4^Pxn.PS

Rpn8^EP687

gamma Kenyon cell | adult stage, with Scer\GAL4^GMR71G10

gamma Kenyon cell | adult stage, with Scer\GAL4^GMR71G10, Spyo\Cas9^UAS.P2

Rpn8^gRNA.pCFD3-1

ganglion mother cell | larval stage

Rpn8^GD10960

larval neuroblast | larval stage

Rpn8^GD10960

mitochondrion | third instar larval stage, with Scer\GAL4^Cg.PA

Rpn8^JF01140

mushroom body gamma-lobe | somatic clone

Rpn8^k08003

neuroblast | somatic clone

Rpn8^k08003

nurse cell ring canal, with Scer\GAL4^{VP16.mat.αTub67C}

Rpn8^GL00333

wing, with Scer\GAL4^bbg-C96

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v7.1)

Homo sapiens (Human) (2)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\PSMD7

14 of 15

Yes

Hsap\EIF3F

1 of 15

Model Organism Orthologs (via DIOPT v7.1)

Mus musculus (laboratory mouse) (1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Psmd7

14 of 15

Yes

Rattus norvegicus (Norway rat) (2)

Rnor\Psmd7

12 of 13

Yes

Rnor\Eif3f

1 of 13

Xenopus tropicalis (Western clawed frog) (2)

Xtro\psmd7

11 of 12

Yes

Xtro\eif3f

1 of 12

Danio rerio (Zebrafish) (2)

Drer\psmd7

10 of 15

Yes

Drer\si:ch211-202f3.4

1 of 15

Caenorhabditis elegans (Nematode, roundworm) (2)

Cele\rpn-8

13 of 15

Yes

Cele\eif-3.F

1 of 15

Arabidopsis thaliana (thale-cress) (3)

Atha\MEE34

9 of 9

Yes

Atha\RPN8A

9 of 9

Yes

Atha\EIF2

1 of 9

Saccharomyces cerevisiae (Brewer's yeast) (1)

Scer\RPN8

14 of 15

Yes

Schizosaccharomyces pombe (Fission yeast) (2)

Spom\rpn8

11 of 12

Yes

Spom\SPBC4C3.07

1 of 12

Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG09190CNL )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Dmel\Rpn8

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA17437

Drosophila persimilis

Dper\GL20068

Drosophila willistoni

Dwil\GK22207

Drosophila virilis

Dvir\GJ21597

Drosophila mojavensis

Dmoj\GI21325

Drosophila grimshawi

Dgri\GH20991

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG0915089I )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17027521

Glossina morsitans

Tsetse fly

Gmor\GMOY005744

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_03221

Mayetiola destructor

Hessian fly

Mdes\Mdes011268

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL000270

Anopheles darlingi

American malaria mosquito

Adar\ADAC002522

Anopheles gambiae

Malaria mosquito

Agam\AGAP012135

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ015055

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0A69 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA005994

Danaus plexippus

Monarch butterfly

Dple\DPOGS214282-PA

Apis florea

Little honeybee

Aflo\3646977028

Apis mellifera

Western honey bee

Amel\LOC408413

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP23615

Bombus terrestris

Buff-tailed bumblebee

Bter\15210220

Linepithema humile

Argentine ant

Lhum\LH16556

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:001797

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG001600

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_07278

Tribolium castaneum

Red flour beetle

Tcas\TC013318

Pediculus humanus

Human body louse

Phum\PHUM077900

Rhodnius prolixus

Kissing bug

Rpro\RPRC007726

Cimex lectularius

Bed bug

Clec\CLEC006425

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI007679

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_09551

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_09553

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X08WI )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR015103

Ixodes scapularis

Black-legged tick

Isca\ISCW022113

Stegodyphus mimosarum

African social velvet spider

Smim\KFM67634.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur26g02250

Tetranychus urticae

Two-spotted spider mite

Turt\tetur469g00010

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_49603

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0DX6 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Psmd7Lp26SnATPase7

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Psmd7

Ciona intestinalis

Vase tunicate

Cint\LOC100183181

Gallus gallus

Domestic chicken

Ggal\PSMD7

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v7.1)

Drosophila melanogaster (Fruit fly) (3)

Gene Symbol

Score

Source

Alignment

3 of 10

3 of 10

2 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

PSMD7; proteasome 26S subunit, non-ATPase 7

14 of 15

157970

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Rpn8 - Prosα2

experimental knowledge based

(Guruharsha et al., 2011)

Rpn8 - Prosα3

experimental knowledge based

(Guruharsha et al., 2011)

Rpn8 - Prosα4

experimental knowledge based

(Guruharsha et al., 2011)

Rpn8 - Prosα5

experimental knowledge based

(Guruharsha et al., 2011)

Rpn8 - Prosα6

experimental knowledge based

(Guruharsha et al., 2011)

Rpn8 - Prosα7

experimental knowledge based

(Guruharsha et al., 2011)

Rpn8 - Prosβ1

experimental knowledge based

(Guruharsha et al., 2011)

Rpn8 - Prosβ2

experimental knowledge based

(Guruharsha et al., 2011)

Rpn8 - Prosβ3

experimental knowledge based

(Guruharsha et al., 2011)

Rpn8 - Prosβ4

experimental knowledge based

(Guruharsha et al., 2011)

Rpn8 - Prosβ5

experimental knowledge based

(Guruharsha et al., 2011)

Rpn8 - Prosβ6

experimental knowledge based

(Guruharsha et al., 2011)

Rpn8 - Prosβ7

experimental knowledge based

(Guruharsha et al., 2011)

Rpn8 - Rpn1

experimental knowledge based

(Guruharsha et al., 2011)

Rpn8 - Rpn11

experimental knowledge based

(Guruharsha et al., 2011)

Rpn8 - Rpn13

experimental knowledge based

(Guruharsha et al., 2011)

Rpn8 - Rpn2

experimental knowledge based

(Guruharsha et al., 2011)

Rpn8 - Rpn3

experimental knowledge based

(Guruharsha et al., 2011)

Rpn8 - Rpn5

experimental knowledge based

(Guruharsha et al., 2011)

Rpn8 - Rpn6

experimental knowledge based

(Guruharsha et al., 2011)

Rpn8 - Rpn7

experimental knowledge based

(Guruharsha et al., 2011)

Rpn8 - Rpn9

experimental knowledge based

(Guruharsha et al., 2011)

Rpn8 - Rpt2

experimental knowledge based

(Guruharsha et al., 2011)

Rpn8 - Rpt3

experimental knowledge based

(Guruharsha et al., 2011)

Rpn8 - Rpt4

experimental knowledge based

(Guruharsha et al., 2011)

Rpn8 - Rpt5

experimental knowledge based

(Guruharsha et al., 2011)

Rpn8 - Rpt6

experimental knowledge based

(Guruharsha et al., 2011)

Summary of Genetic Interactions

esyN Network Diagram

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

63468

DroID - A comprehensive database of gene and protein interactions.

FBgn0002787

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

37894

MIST (protein-protein) - An integrated Molecular Interaction Database

Rpn8

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Proteasome

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-106

Cytogenetic map

60C8-60C8

Sequence location

2R:24,400,028..24,404,527 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

60C8-60C8

Limits computationally determined from genome sequence between P{PZ}bs⁰³²⁶⁷&P{lacW}l(2)k10502^k10502 and P{lacW}Nurf-38^k16102

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

60D1-60D2

(determined by in situ hybridisation)

(Spradling et al., 1999)

60D1-60D2

(Beaton, 1997.12.27, BDGP Project Members, 1994-1999)

60B-60C

(determined by in situ hybridisation)

(Gridley et al., 1990)

Experimentally Determined Recombination Data

Location

2-106

(FlyBase, 2016)

2-110.6

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (12)

Bloomington

31567

y¹ v¹; P{TRiP.JF01140}attP2

43349

w^*; P{GSV1}Rpn8^C125 Slik^C125

23860

y¹ w^*; P{neoFRT}42D P{lacW}Rpn8^k08003/CyO

35411

y¹ sc^* v¹ sev²¹; P{TRiP.GL00333}attP2

67913

y¹ sc^* v¹ sev²¹; P{TRiP.HMS05743}attP40

83260

y¹ w^*; TI{CRIMIC.TG4.0}Slik^{CR01310-TG4.0} Rpn8^{CR01310-TG4.0-X}/SM6a

FlyORF

F001349

M{UAS-Rpn8.ORF}ZH-86Fb

F000795

M{UAS-Rpn8.ORF.3xHA}ZH-86Fb

Kyoto

103035

y¹ w^67c23 ; P{lacW}Rpn8^k16724 / CyO

111449

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{neoFRT}42D P{lacW}Rpn8^k08003 /CyO y⁺

VDRC

v108573

P{KK101319}VIE-260B

v26183

w¹¹¹⁸ P{GD10960}v26183

Genomic Clones (16)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (193)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequences

BDGP DGC clones

AT07973

Other clones

DPiM_AT07973

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0002787

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0002787

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

37894

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: Mov34 CG3416

(Wang, 1999.11)

Source for identity of: Rpn8 Mov34

(FlyBase, 2009-)

Source for database merge of

Source for merge of: Mov34 l(2)k08003

(Spradling et al., 1999)

Additional comments

The nomenclature of genes encoding subunits of the 26S proteasome of D. melanogaster have been standardized according to FBrf0215459. These symbols/names largely follow those used already in FlyBase, and largely mirror fly community usage. HOWEVER, note that at least one other nomenclature system exists that is followed by the HUGO Gene Nomenclature Committee (HGNC), for example, with the unfortunate result that several D. melanogaster genes have shared synonyms.

(FlyBase, 2009-)

Other Comments

RNAi screen using dsRNA made from templates generated with primers directed against this gene results in the formation of short, monastral bipolar spindles and severe proliferation defects when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.

(Goshima et al., 2007)

RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R⁺ cells.

(Boutros et al., 2004)

RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R⁺ cells.

(Boutros et al., 2004)

The 26S proteolytic complex, a multiprotein complex containing many subunits (including those encoded by Pros26.4, Pros54 and Mov34), can be assembled from two smaller multiprotein complexes, the μ particle and the 20S proteasome, by a process that requires ATP.

(Udvardy, 1993)

Mov34 has been cloned and analysed.

(Gridley et al., 1990)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 91 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

37894

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P26270

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0002787

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0002787

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG3416

Flygut - An atlas of the Drosophila adult midgut

FBgn0002787

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

99116

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

37894

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG3416

modMine - A data warehouse for the modENCODE project

FBgn0002787

Linkouts

BioGRID - A database of protein and genetic interactions.

63468

DPiM - Drosophila Protein interaction map

FBgn0002787

DroID - A comprehensive database of gene and protein interactions.

FBgn0002787

DRSC - Results frm RNAi screens

FBgn0002787

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0002787

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG3416-RA

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0002787

FlyMine - An integrated database for Drosophila genomics

FBgn0002787

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

37894

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Proteasome

MIST (protein-protein) - An integrated Molecular Interaction Database

Rpn8

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Synonyms and Secondary IDs (11)

Reported As

Symbol Synonym

P26270

(Clague et al., 2013)

PRSC_DROME

(Aravind and Ponting, 1998)

PSMD7

(Tsou et al., 2015)

RPN8

(Gemmill et al., 2005)

Rpn8

(Kurshakova et al., 2019, Meltzer et al., 2019, Zhou et al., 2019, Gene Disruption Project members, 2018-, Cook, 2015.12.23, Hudson et al., 2015, Wang et al., 2014, Pathare et al., 2012, Lee et al., 2010, Belote and Zhong, 2009, Nickell et al., 2009, Lundgren et al., 2005, Ma et al., 2002)

S12

(Lundgren et al., 2005)

l(2)k08003

(Beaton, 1997.12.27, BDGP Project Members, 1994-1999)

p39B

(Sumegi et al., 2003, Kurucz et al., 2002, Ma et al., 2002, Holzl et al., 2000)

Name Synonyms

Mov34

(Cammarato et al., 2011)

Regulatory particle non-ATPase 8

Secondary FlyBase IDs

FBgn0022065

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (102)

Report Sections

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