FlyBase Gene Report: Dmel\Rpn8

General Information

Symbol

Dmel\Rpn8

Species

D. melanogaster

Name

Regulatory particle non-ATPase 8

Annotation Symbol

CG3416

Feature Type

protein_coding_gene

FlyBase ID

FBgn0002787

Gene Model Status

Current

Stock Availability

12 publicly available

Gene Summary

Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins. (UniProt, P26270)

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

Mov34, p39B, l(2)k08003

Key Links

Genomic Location

Cytogenetic map

60C8-60C8

Sequence location

2R:24,400,028..24,404,527 [+]

Recombination map

2-106

RefSeq locus

NT_033778 REGION:24400028..24404527

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (8 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

enables isopeptidase activity

inferred from electronic annotation with InterPro:IPR000555

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

NOT enables metal-dependent deubiquitinase activity

inferred from sequence or structural similarity with UniProtKB:P51665

(FlyBase Curators, 2008-)

NOT enables peptidase activity

inferred from sequence or structural similarity with UniProtKB:P51665

(FlyBase Curators, 2008-)

Biological Process (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

involved_in proteasome-mediated ubiquitin-dependent protein catabolic process

inferred from mutant phenotype

(Lőw et al., 2013)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in proteasome-mediated ubiquitin-dependent protein catabolic process

inferred from biological aspect of ancestor with PANTHER:PTN000057040

(assigned by GO_Central )

(Gaudet et al., 2011)

inferred by curator from GO:0005838

(Sumegi et al., 2003)

Cellular Component (4 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

located_in dendrite

inferred from mutant phenotype

(Kuo et al., 2005)

part_of proteasome complex

inferred from direct assay

(Nickell et al., 2009)

part_of proteasome regulatory particle

inferred from direct assay

(Sumegi et al., 2003, Holzl et al., 2000)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

part_of proteasome complex

inferred from biological aspect of ancestor with PANTHER:PTN000057040

(assigned by GO_Central )

(Gaudet et al., 2011)

part_of proteasome regulatory particle, lid subcomplex

inferred from sequence or structural similarity with SGD:S000005787

(Holzl et al., 2000)

part_of proteasome regulatory particle

inferred from sequence or structural similarity with HGNC:9565

(Holzl et al., 2000)

Gene Group (FlyBase)

M67 NON-PEPTIDASE HOMOLOGS

PROTEASOME REGULATORY PARTICLE NON-ATPASES

Protein Family (UniProt)

Belongs to the peptidase M67A family. (P26270)

Protein Signatures (InterPro)

Summaries

Gene Group (FlyBase)

M67 NON-PEPTIDASE HOMOLOGS -: M67 non-peptidase homologs are similar in amino acid sequence to JAMM deubiquitinases (M67 metallopeptidases) but lack one or more of the expected catalytic residues or zinc-binding sites. They may act as scaffold proteins or as ubiquitin-binding modules within complexes. (Adapted from FBrf0239469 and PMID:17559875.)
PROTEASOME REGULATORY PARTICLE NON-ATPASES -: The 26S proteasome degrades polyubiquitinated proteins in the cytoplasm and nucleus. It is formed from a cylindrical catalytic core 20S proteasome capped at each end by a regulatory 19S particle. The 19S regulatory particle recognizes, unfolds, and translocates ubiquitinated proteins into the catalytic core particle in an ATP-dependent manner. 'Rpn' subunits are non-ATPase subunits located in the base and lid subcomplexes of the regulatory particle (Adapted from FBrf0215459 and PMID:28583440.)

Protein Function (UniProtKB)

Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.

(UniProt, P26270)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Rpn8 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure New Section

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry P26270)

If you don't see the viewer to the right, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Comments on Gene Model

Gene model reviewed during 5.44

Stop-codon suppression (UAG) postulated; FBrf0216884

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.46

Sequence Ontology: Class of Gene

gene_with_stop_codon_read_through

(Jungreis et al., 2011)

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Rpn8-RA

FBtr0072290

NM_079121

1456

338

Rpn8-RB

FBtr0330237

NM_001274238

1456

358

Rpn8-RC

FBtr0332921

NM_001274239

1473

338

Additional Transcript Data and Comments

Reported size (kB)

1.4 (northern blot)

(Gridley et al., 1990)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Rpn8-PA

FBpp0072197

38.1

338

6.69

FBpp0303270

40.6

358

9.55

FBpp0305141

38.1

338

6.69

P26270

NP_001261168

AGB93698

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

338 aa isoforms: Rpn8-PA, Rpn8-PC

Additional Polypeptide Data and Comments

Reported size (kDa)

338 (aa)

(Gridley et al., 1990)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Rpn8 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that the Fly Cell Atlas project found the gene expressed in that cell type. Darker colors mean that more cells of that cell type express the gene:

low

high

Colorless tiles indicate that there is no scRNAseq data for the gene in that cell type.

Colored tiles in ribbon indicate that expression data (RNA and/or protein) has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

Colored tiles in the ribbon indicate the average RNA expression level of the gene at the indicated stages:

low

high

as determined by RNA-seq (RPKM) using whole organism samples modENCODE, Brown et al., 2014. For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

pole plasm

Comment: maternally deposited

(Fisher et al., 2012)

organism

• pole plasm

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

primordial germ cell

(Fisher et al., 2012)

embryonic stage 7 -- 8

anterior endoderm anlage

(Fisher et al., 2012)

head mesoderm

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

primordial germ cell

(Fisher et al., 2012)

posterior endoderm

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

embryonic stage 9 -- 10

anterior endoderm

(Fisher et al., 2012)

head mesoderm

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

posterior endoderm

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

embryonic stage 11 -- 12

anterior midgut primordium

(Fisher et al., 2012)

central brain primordium

(Fisher et al., 2012)

head mesoderm

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

posterior midgut primordium

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

ventral nerve cord primordium

(Fisher et al., 2012)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

(Gridley et al., 1990)

Additional Descriptive Data

The 1.4 kb transcript is detected in adult heads and bodies.

(Gridley et al., 1990)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in dendrite

inferred from mutant phenotype

(Kuo et al., 2005)

part_of proteasome complex

inferred from direct assay

(Nickell et al., 2009)

part_of proteasome regulatory particle

inferred from direct assay

(Sumegi et al., 2003, Holzl et al., 2000)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Rpn8 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Rpn8

View exonic expression by tissue for Dmel\Rpn8

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0002787

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

37894/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0002787

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0002787

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0002787

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG3416

Flygut - An atlas of the Drosophila adult midgut

FBgn0002787

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\Rpn8

Stages(s) 4-6
Stages(s) 7-8
Stages(s) 9-10
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 5 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 11 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Rpn8

Transgenic constructs containing regulatory region of Rpn8

Aberrations (Deficiencies and Duplications) ( 6 )

Inferred from experimentation ( 6 )

Gene disrupted in

Df(2R)BSC603

(Kahsai et al., 2016, Christensen et al., 2008.9.3)

Df(2R)BSC780

(Christensen et al., 2009.5.6)

Df(2R)bw^VDe2LPx^KR

(Spradling et al., 1999, Beaton, 1997.12.27)

Df(2R)ED4061

(Kahsai et al., 2016)

Df(2R)Exel6278

(Ryder, 2004.4.26)

In(2LR)Px⁴

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

Inferred from location ( 0 )

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal

Rpn8^GD10960

lethal, with Scer\GAL4^GMR71G10, Spyo\Cas9^UAS.P2

lethal, with Scer\GAL4^GMR71G10, Spyo\Cas9^{UAS.Tag:NLS(Unk)}

lethal, with Scer\GAL4^pnr-MD237

Rpn8^GD10960

lethal, with Spyo\Cas9^{UAS.Tag:NLS(Unk)}

lethal (with Df(2R)BSC603)

Rpn8^k08003

lethal (with Df(2R)ED4061)

Rpn8^k08003

lethal | larval stage, with Scer\GAL4^sqh.PW

Rpn8^KK101319

lethal | pharate adult stage, with Scer\GAL4^elav.PU

Rpn8^KK101319

lethal | pupal stage, with Scer\GAL4^elav.PU

Rpn8^KK101319

lethal | pupal stage, with Scer\GAL4^sqh.PW

Rpn8^KK101319

lethal | recessive

lethal - all die before end of pupal stage, with Scer\GAL4^dpp.PU

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Rpn8^GD10960

viable, with Scer\GAL4^GMR71G10, Spyo\Cas9^UAS.P2

Rpn8^gRNA.pCFD3-1

Other Phenotypes

Allele

abnormal neuroanatomy | adult stage, with Scer\GAL4^GMR71G10

abnormal neuroanatomy | adult stage, with Scer\GAL4^GMR71G10, Spyo\Cas9^UAS.P2

Rpn8^gRNA.pCFD3-1

abnormal neuroanatomy | larval stage

Rpn8^GD10960

abnormal neuroanatomy | pupal stage

Rpn8^k08003

abnormal neuroanatomy | somatic clone

Rpn8^k08003

decreased cell number | somatic clone

Rpn8^k08003

decreased fecundity | female, with Scer\GAL4^{VP16.mat.αTub67C}

Rpn8^GL00333

some die during pupal stage, with Scer\GAL4^dpp.PU

visible, with Scer\GAL4^bbg-C96

Phenotype manifest in

Allele

actin cytoskeleton | adult stage | female, with Scer\GAL4^{VP16.mat.αTub67C}

Rpn8^GL00333

adult mushroom body | somatic clone

Rpn8^k08003

adult mushroom body gamma-lobe | somatic clone

Rpn8^k08003

axon | adult stage, with Scer\GAL4^GMR71G10

axon | adult stage, with Scer\GAL4^GMR71G10, Spyo\Cas9^UAS.P2

Rpn8^gRNA.pCFD3-1

dendritic arborizing neuron | P-stage

Rpn8^k08003

egg chamber, with Scer\GAL4^{VP16.mat.αTub67C}

Rpn8^GL00333

embryonic/larval fat body | third instar larval stage, with Scer\GAL4^Cg.PA

Rpn8^JF01140

embryonic/larval hemocyte | third instar larval stage, with Scer\GAL4^Pxn.PS

Rpn8^EP687

gamma Kenyon cell | adult stage, with Scer\GAL4^GMR71G10

gamma Kenyon cell | adult stage, with Scer\GAL4^GMR71G10, Spyo\Cas9^UAS.P2

Rpn8^gRNA.pCFD3-1

ganglion mother cell | larval stage

Rpn8^GD10960

larval multidendritic class IV neuron, with Scer\GAL4^ppk.PU

Rpn8^GD10960

larval neuroblast | larval stage

Rpn8^GD10960

mitochondrion | third instar larval stage, with Scer\GAL4^Cg.PA

Rpn8^JF01140

neuroblast | somatic clone

Rpn8^k08003

nurse cell ring canal, with Scer\GAL4^{VP16.mat.αTub67C}

Rpn8^GL00333

wing, with Scer\GAL4^bbg-C96

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (10)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\PSMD7

14 of 15

Yes

Hsap\BRCC3

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (10)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Psmd7

14 of 15

Yes

Mmus\Brcc3

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Rattus norvegicus (Norway rat) (9)

Rnor\Psmd7

12 of 13

Yes

Rnor\Eif3f

2 of 13

Rnor\Brcc3

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Xenopus tropicalis (Western clawed frog) (10)

Xtro\psmd7

12 of 12

Yes

Xtro\eif3f

2 of 12

Xtro\brcc3

1 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Danio rerio (Zebrafish) (10)

Drer\psmd7

11 of 15

Yes

Drer\eif3f

2 of 15

Drer\brcc3

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Caenorhabditis elegans (Nematode, roundworm) (5)

Cele\rpn-8

14 of 15

Yes

2 of 15

1 of 15

1 of 15

1 of 15

Arabidopsis thaliana (thale-cress) (2)

Atha\MEE34

9 of 9

Yes

Atha\RPN8A

9 of 9

Yes

Saccharomyces cerevisiae (Brewer's yeast) (3)

Scer\RPN8

13 of 15

Yes

Scer\RPN11

1 of 15

Scer\RRI1

1 of 15

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\rpn8

10 of 12

Yes

Other Organism Orthologs (via OrthoDB)

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (7)

Gene Symbol

Score

Source

Alignment

4 of 10

4 of 10

3 of 10

1 of 10

1 of 10

1 of 10

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

congenital heart defect (postulated), PSMD7-related

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Allele

Disease

Evidence

References

Rpn8^KK101319

model of congenital heart disease

CEA

(Nim et al., 2021)

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

PSMD7; proteasome 26S subunit, non-ATPase 7

14 of 15

157970

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Rpn8 - Prosα2

experimental knowledge based

(Guruharsha et al., 2011)

Rpn8 - Prosα3

experimental knowledge based

(Guruharsha et al., 2011)

Rpn8 - Prosα4

experimental knowledge based

(Guruharsha et al., 2011)

Rpn8 - Prosα5

experimental knowledge based

(Guruharsha et al., 2011)

Rpn8 - Prosα6

experimental knowledge based

(Guruharsha et al., 2011)

Rpn8 - Prosα7

experimental knowledge based

(Guruharsha et al., 2011)

Rpn8 - Prosβ1

experimental knowledge based

(Guruharsha et al., 2011)

Rpn8 - Prosβ2

experimental knowledge based

(Guruharsha et al., 2011)

Rpn8 - Prosβ3

experimental knowledge based

(Guruharsha et al., 2011)

Rpn8 - Prosβ4

experimental knowledge based

(Guruharsha et al., 2011)

Rpn8 - Prosβ5

experimental knowledge based

(Guruharsha et al., 2011)

Rpn8 - Prosβ6

experimental knowledge based

(Guruharsha et al., 2011)

Rpn8 - Prosβ7

experimental knowledge based

(Guruharsha et al., 2011)

Rpn8 - Rpn1

experimental knowledge based

(Guruharsha et al., 2011)

Rpn8 - Rpn11

experimental knowledge based

(Guruharsha et al., 2011)

Rpn8 - Rpn13

experimental knowledge based

(Guruharsha et al., 2011)

Rpn8 - Rpn2

experimental knowledge based

(Guruharsha et al., 2011)

Rpn8 - Rpn3

experimental knowledge based

(Guruharsha et al., 2011)

Rpn8 - Rpn5

experimental knowledge based

(Guruharsha et al., 2011)

Rpn8 - Rpn6

experimental knowledge based

(Guruharsha et al., 2011)

Rpn8 - Rpn7

experimental knowledge based

(Guruharsha et al., 2011)

Rpn8 - Rpn9

experimental knowledge based

(Guruharsha et al., 2011)

Rpn8 - Rpt2

experimental knowledge based

(Guruharsha et al., 2011)

Rpn8 - Rpt3

experimental knowledge based

(Guruharsha et al., 2011)

Rpn8 - Rpt4

experimental knowledge based

(Guruharsha et al., 2011)

Rpn8 - Rpt5

experimental knowledge based

(Guruharsha et al., 2011)

Rpn8 - Rpt6

experimental knowledge based

(Guruharsha et al., 2011)

Summary of Genetic Interactions

esyN Network Diagram

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

63468

DroID - A comprehensive database of gene and protein interactions.

FBgn0002787

MIST (protein-protein) - An integrated Molecular Interaction Database

Rpn8

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Proteasome

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-106

Cytogenetic map

60C8-60C8

Sequence location

2R:24,400,028..24,404,527 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

60C8-60C8

Limits computationally determined from genome sequence between P{PZ}bs⁰³²⁶⁷&P{lacW}l(2)k10502^k10502 and P{lacW}Nurf-38^k16102

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

60D1-60D2

(determined by in situ hybridisation)

(Spradling et al., 1999)

60D1-60D2

(Beaton, 1997.12.27, BDGP Project Members, 1994-1999)

60B-60C

(determined by in situ hybridisation)

(Gridley et al., 1990)

Experimentally Determined Recombination Data

Location

2-106

(FlyBase, 2016)

2-110.6

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (12)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

31567

y¹ v¹; P{TRiP.JF01140}attP2

43349

w^*; P{GSV1}Rpn8^C125 Slik^C125

23860

y¹ w^*; P{neoFRT}42D P{lacW}Rpn8^k08003/CyO

35411

y¹ sc^* v¹ sev²¹; P{TRiP.GL00333}attP2

67913

y¹ sc^* v¹ sev²¹; P{TRiP.HMS05743}attP40

83260

y¹ w^*; TI{CRIMIC.TG4.0}Slik^{CR01310-TG4.0} Rpn8^{CR01310-TG4.0-X}/SM6a

FlyORF

F001349

M{UAS-Rpn8.ORF}ZH-86Fb

F000795

M{UAS-Rpn8.ORF.3xHA}ZH-86Fb

Kyoto

103035

y¹ w^67c23 ; P{lacW}Rpn8^k16724 / CyO

111449

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{neoFRT}42D P{lacW}Rpn8^k08003 /CyO y⁺

VDRC

v108573

P{KK101319}VIE-260B

v26183

w¹¹¹⁸ P{GD10960}v26183

Genomic Clones (16)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (193)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

AT07973

Other clones

DPiM_AT07973

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0002787

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0002787

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

37894

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: Mov34 CG3416

(Wang, 1999.11)

Source for identity of: Rpn8 Mov34

(FlyBase, 2009-)

Source for database merge of

Source for merge of: Mov34 l(2)k08003

(Spradling et al., 1999)

Additional comments

The nomenclature of genes encoding subunits of the 26S proteasome of D. melanogaster have been standardized according to FBrf0215459. These symbols/names largely follow those used already in FlyBase, and largely mirror fly community usage. HOWEVER, note that at least one other nomenclature system exists that is followed by the HUGO Gene Nomenclature Committee (HGNC), for example, with the unfortunate result that several D. melanogaster genes have shared synonyms.

(FlyBase, 2009-)

Other Comments

RNAi screen using dsRNA made from templates generated with primers directed against this gene results in the formation of short, monastral bipolar spindles and severe proliferation defects when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.

(Goshima et al., 2007)

RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R⁺ cells.

(Boutros et al., 2004)

RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R⁺ cells.

(Boutros et al., 2004)

The 26S proteolytic complex, a multiprotein complex containing many subunits (including those encoded by Pros26.4, Pros54 and Mov34), can be assembled from two smaller multiprotein complexes, the μ particle and the 20S proteasome, by a process that requires ATP.

(Udvardy, 1993)

Mov34 has been cloned and analysed.

(Gridley et al., 1990)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 94 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

37894

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

P26270

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P26270

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

P26270

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0002787

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0002787

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

37894/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0002787

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0002787

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0002787

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG3416

Flygut - An atlas of the Drosophila adult midgut

FBgn0002787

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

99116

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

37894

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG3416

MARRVEL_MODEL - MARRVEL (model organism gene)

37894

modMine - A data warehouse for the modENCODE project

FBgn0002787

Linkouts

BioGRID - A database of protein and genetic interactions.

63468

DroID - A comprehensive database of gene and protein interactions.

FBgn0002787

DRSC - Results frm RNAi screens

FBgn0002787

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0002787

FlyMine - An integrated database for Drosophila genomics

FBgn0002787

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Proteasome

MIST (protein-protein) - An integrated Molecular Interaction Database

Rpn8

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Synonyms and Secondary IDs (12)

Reported As

Symbol Synonym

P26270

(Clague et al., 2013)

PRSC_DROME

(Aravind and Ponting, 1998)

PSMD7

(Tsou et al., 2015)

RPN8

(Gemmill et al., 2005)

Rpn8

(Parkhitko et al., 2021, Fernández-Cruz et al., 2020, Walls et al., 2020, Wei et al., 2020, Kurshakova et al., 2019, Meltzer et al., 2019, Zhou et al., 2019, Gene Disruption Project members, 2018-, Cook, 2015.12.23, Hudson et al., 2015, Wang et al., 2014, Pathare et al., 2012, Lee et al., 2010, Belote and Zhong, 2009, Nickell et al., 2009, Lundgren et al., 2005, Ma et al., 2002)

S12

(Lundgren et al., 2005)

l(2)k08003

(Beaton, 1997.12.27, BDGP Project Members, 1994-1999)

mov34

(Chew et al., 2021)

p39B

(Sumegi et al., 2003, Kurucz et al., 2002, Ma et al., 2002, Holzl et al., 2000)

Name Synonyms

Mov34

(Cammarato et al., 2011)

Regulatory particle non-ATPase 8

Secondary FlyBase IDs

FBgn0022065

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

References (114)

The following external sites may use different assemblies or annotations than FlyBase.

Report Sections