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Gene Dmel\Rpn8

General Information
Symbol	Dmel\Rpn8	Species	D. melanogaster
Name	Regulatory particle non-ATPase 8	Annotation symbol	CG3416
Feature type	protein_coding_gene	FlyBase ID	FBgn0002787
Gene Model Status	Current	Stock availability	7 publicly available
Also Known As	Mov34, p39B, l(2)k08003
Genomic Location
Chromosome (arm)	2R	Recombination map
Cytogenetic map	60C8-60C8	Sequence location	2R:20,287,551..20,292,050 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Regulatory particle non-ATPase 8 is referred to in FlyBase by the symbol Dmel\Rpn8 (CG3416, FBgn0002787). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: endopeptidase activity. There is experimental evidence that it is involved in the biological process: mitotic spindle elongation; cell proliferation; cellular process; mitotic spindle organization; proteolysis; neurogenesis. 8 alleles are reported. No phenotypic data is available. It has 3 annotated transcripts and 3 annotated polypeptides. Protein features are: JAB1/Mov34/MPN/PAD-1; Rpn11/EIF3F C-terminal domain. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of moderately high expression. Peak expression observed within 00-06 hour embryonic stages, during late larval stages, at stages throughout the pupal period, in adult female stages. Summary of FlyAtlas Anatomical Expression Data: High or moderate levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: larval central nervous system, adult crop, larval midgut, larval hindgut, larval Malpighian tubules, adult heart, adult fat body, larval salivary gland, larval trachea, adult ovary, larval/adult carcass. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, adult central nervous system, adult midgut, adult hindgut, adult Malpighian tubules, larval fat body, adult salivary gland, adult spermathecae, adult male reproductive system. Comments on Affy2 ProbeSet: ProbeSet 1635259_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of Mov34. Gene sequence location is 2R:20287551..20292050.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 60C8-60C8   Limits computationally determined from genome sequence between P{PZ}bs03267&P{lacW}l(2)k10502k10502 and P{lacW}Nurf-38k16102
Experimentally Determined Cytological Location
Cytogenetic map Notes References 60D1-60D2   (determined by in situ hybridisation)   (Spradling et al., 1999) 60D1-60D2     (Beaton, 1997.12.27) 60D1-60D2     (BDGP Project Members, 1994-1999) 60B-60C   (determined by in situ hybridisation)   (Gridley et al., 1990)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Rpn8 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.44 Stop-codon suppression (UAG) postulated; FBrf0216884 gene_with_stop_codon_read_through ; SO:0000697 Low-frequency RNA-Seq exon junction(s) not annotated. Gene model reviewed during 5.46
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Rpn8-RA FBtr0072290  NM_079121   1456   338 Rpn8-RB FBtr0330237  NM_001274238   1456   358 Rpn8-RC FBtr0332921  NM_001274239   1473   338
Additional Transcript Data & Comments
Reported size (kB)	1.4 (northern blot) (Gridley et al., 1990)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Rpn8-PA   FBpp0072197   38.1   338   6.69     NP_523845   AAF47199 Rpn8-PB   FBpp0303270   40.6   358   9.55     NP_001261167   AGB93697 Rpn8-PC   FBpp0305141   38.1   338   6.69     NP_001261168   AGB93698
Additional Polypeptide Data & Comments
Reported size (kDa)	338 (aa) (Gridley et al., 1990)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Rpn11/EIF3F C-terminal domain (IPR024969) JAB1/Mov34/MPN/PAD-1 (IPR000555)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA264753   AA538583   AA567250   AA696464   AA697688   AA735720   AA802012   AA803416   AA940656   AE013599 AAF47199   AE013599 AGB93697   AE013599 AGB93698   AI297496   AI456439   AI513947   AQ025851   AY089283 AAL90021   BE978611   BF486781   BF489180   BF497901   BF499256   BF500729   BF502520   BF505696   BI173108   BI231435   BI580006   CK132487   CK661108   CO307260   EC066082   GH749204   GH749866   GH750916   GH752238   GH752890   GH753198   GH754036   GH754305   GH754587   GH754868   GH755593   GH759483   GH759721   GH761069   GH770369   GH771413   GH772216   GH773153   GH774704   GH776573   GH778081   GH778133   GH780535   GH780583   GH780974   GH782898   GH783296   GH783625   GH785087   GH787023   GH789233   GH789768   GH791047   GH791787   GH792503   GH792885   GH793383   GH795334   GH798043   GH801207   GH802506   GH802542   GH803274   GH803993   GH806473   GH809315   GH810323   GH810554   GH811027   GH812484   GH812847   GH814231   GH815840   GH816068   GH816184   GH817666   GH817690   GH818972   GH819066   GH819468   GH819504   GH819611   GH822019   GH822437   GH823621   GH824109   GH824662   GH824674   GH824842   GH828597   GH829246   GH829374   GH831199   GH832377   GH832609   GH832761   GH832938   GH834425   GH838081   GH838176   GH838521   GH845048   GH847639   GH847882   GH848923   GH850959   GH851946   GH852672   GH853950   GH854094   GH854294   GH854957   GH855663   GH856626   GH856736   GH857658   GH858085   GH858460   GH858666   GH858702   GH860294   GH861052   GH864613   GH864725   GH864900   GH865248   GH865703   GH865982   GH867451   GH867906   GH872203   GH872379   GH874909   GH875022   GH875023   GH875971   GH876869   GH877137   GH878934   GH881597   GH882785   GH883183   GH883597   GH885331   GH885721   GH886976   GH887774   GH888988   GH890725   GH892070   GH893098   GH895023   GH896596   GH898708   GH899939   GH900185   GH900187   GH900220   GH900248   GH902625   GH902707   GH903710   GH904538   GH905582   GH908554   GH909392   GH910286   GH911099   GH914026   GH915040   GH917603   GH917687   GH919475   GH920816   GH921377   GH922415   GH927239   GH928440   GH929761   GH930287   GH930291   GH933113   GH933163   GH938512   GH942520   GH954647   GH955371   GH955443   GH957520   GH962077   GH969133   GH970987   GH971361   GH975261   GH984280   M64643 AAA28695
UniProtKB/Swiss-Prot	P26270
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   pole plasm   (Fisher et al., 2012) Comment:maternally deposited organism   • pole plasm (Fisher et al., 2012) Comment:maternally deposited embryonic stage 4 -- 6   organism | ubiquitous   (Fisher et al., 2012) pole cell   (Fisher et al., 2012) embryonic stage 7 -- 8   anterior endoderm anlage   (Fisher et al., 2012) head mesoderm   (Fisher et al., 2012) organism | ubiquitous   (Fisher et al., 2012) pole cell   (Fisher et al., 2012) posterior endoderm   (Fisher et al., 2012) trunk mesoderm   (Fisher et al., 2012) embryonic stage 9 -- 10   anterior endoderm   (Fisher et al., 2012) head mesoderm   (Fisher et al., 2012) organism | ubiquitous   (Fisher et al., 2012) posterior endoderm   (Fisher et al., 2012) trunk mesoderm   (Fisher et al., 2012) embryonic stage 11 -- 12   anterior midgut primordium   (Fisher et al., 2012) central brain primordium   (Fisher et al., 2012) head mesoderm   (Fisher et al., 2012) organism | ubiquitous   (Fisher et al., 2012) posterior midgut primordium   (Fisher et al., 2012) trunk mesoderm   (Fisher et al., 2012) ventral nerve cord primordium   (Fisher et al., 2012) embryonic stage 13 -- 16   embryonic brain   (Fisher et al., 2012) embryonic/larval midgut   (Fisher et al., 2012) embryonic/larval nervous system   (Fisher et al., 2012) gonad   (Fisher et al., 2012) lateral cord neuron   (Fisher et al., 2012) organism | faint & ubiquitous   (Fisher et al., 2012) organism | ubiquitous   (Fisher et al., 2012) ventral nerve cord   (Fisher et al., 2012) northern blot Stage Tissue/Position (including subcellular localization) Reference adult stage     (Gridley et al., 1990)
Additional Descriptive Data
	The 1.4 kb transcript is detected in adult heads and bodies. (Gridley et al., 1990)
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy Stage Tissue/Position (including subcellular localization) Reference day 7 of adulthood -- day 49 of adulthood   adult heart   (Cammarato et al., 2011)
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
dendrite inferred from mutant phenotype (Kuo et al., 2005) proteasome complex inferred from direct assay (Udvardy, 1993) proteasome regulatory particle inferred from direct assay (Sumegi et al., 2003) inferred from direct assay AND inferred from sequence or structural similarity with human PSMD7 (Holzl et al., 2000)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: High or moderate levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: larval central nervous system, adult crop, larval midgut, larval hindgut, larval Malpighian tubules, adult heart, adult fat body, larval salivary gland, larval trachea, adult ovary, larval/adult carcass. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, adult central nervous system, adult midgut, adult hindgut, adult Malpighian tubules, larval fat body, adult salivary gland, adult spermathecae, adult male reproductive system. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    650.625 Larval Midgut    739.6 Larval Hindgut    741.4 Larval Malpighian Tubules    1196.7 Larval Fat Body    438.8 Larval Salivary Gland    723.2 Larval Trachea    543.15 Larval Carcass    1282.3 Adult Head    296.1 Adult Eye    300.45 Adult Brain    305.3 Adult Thoracic-Abdominal Ganglion    311.5 Adult Crop    595.8 Adult Midgut    428.9 Adult Hindgut    417.1 Adult Malpighian Tubules    421.8 Adult Fat Body    631.3 Adult Salivary Gland    364.1 Adult Heart    784.25 Adult VirginFemale Spermatheca    387.6 Adult InseminatedFemale Spermatheca    384.2 Adult Ovary    676.7 Adult Testis    426.3 Adult Male Accessory Gland    305 Adult Carcass    528.2 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  (650.625) Larval Midgut  (739.6) Larval Hindgut  (741.4) Larval Malpighian Tubules  (1196.7) Larval Fat Body    438.8 Larval Salivary Gland  (723.2) Larval Trachea  (543.15) Larval Carcass  (1282.3) Adult Head    296.1 Adult Eye    300.45 Adult Brain    305.3 Adult Thoracic-Abdominal Ganglion    311.5 Adult Crop  (595.8) Adult Midgut    428.9 Adult Hindgut    417.1 Adult Malpighian Tubules    421.8 Adult Fat Body  (631.3) Adult Salivary Gland    364.1 Adult Heart  (784.25) Adult VirginFemale Spermatheca    387.6 Adult InseminatedFemale Spermatheca    384.2 Adult Ovary  (676.7) Adult Testis    426.3 Adult Male Accessory Gland    305 Adult Carcass  528.2 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    650.625 Larval Midgut    739.6 Larval Hindgut    741.4 Larval Malpighian Tubules  (1196.7) Larval Fat Body    438.8 Larval Salivary Gland    723.2 Larval Trachea    543.15 Larval Carcass  (1282.3) Adult Head    296.1 Adult Eye    300.45 Adult Brain    305.3 Adult Thoracic-Abdominal Ganglion    311.5 Adult Crop    595.8 Adult Midgut    428.9 Adult Hindgut    417.1 Adult Malpighian Tubules    421.8 Adult Fat Body    631.3 Adult Salivary Gland    364.1 Adult Heart    784.25 Adult VirginFemale Spermatheca    387.6 Adult InseminatedFemale Spermatheca    384.2 Adult Ovary    676.7 Adult Testis    426.3 Adult Male Accessory Gland    305 Adult Carcass    528.2 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    650.625 Larval Midgut    739.6 Larval Hindgut    741.4 Larval Malpighian Tubules    1196.7 Larval Fat Body    438.8 Larval Salivary Gland    723.2 Larval Trachea    543.15 Larval Carcass    1282.3 Adult Head    296.1 Adult Eye    300.45 Adult Brain    305.3 Adult Thoracic-Abdominal Ganglion    311.5 Adult Crop    595.8 Adult Midgut    428.9 Adult Hindgut    417.1 Adult Malpighian Tubules    421.8 Adult Fat Body    631.3 Adult Salivary Gland    364.1 Adult Heart    784.25 Adult VirginFemale Spermatheca    387.6 Adult InseminatedFemale Spermatheca    384.2 Adult Ovary    676.7 Adult Testis    426.3 Adult Male Accessory Gland    305 Adult Carcass    528.2 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    650.625 Larval Midgut    739.6 Larval Hindgut    741.4 Larval Malpighian Tubules    1196.7 Larval Fat Body    438.8 Larval Salivary Gland    723.2 Larval Trachea    543.15 Larval Carcass    1282.3 Adult Head    296.1 Adult Eye    300.45 Adult Brain    305.3 Adult Thoracic-Abdominal Ganglion    311.5 Adult Crop    595.8 Adult Midgut    428.9 Adult Hindgut    417.1 Adult Malpighian Tubules    421.8 Adult Fat Body    631.3 Adult Salivary Gland    364.1 Adult Heart    784.25 Adult VirginFemale Spermatheca    387.6 Adult InseminatedFemale Spermatheca    384.2 Adult Ovary    676.7 Adult Testis    426.3 Adult Male Accessory Gland    305 Adult Carcass    528.2 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  650.625 Larval Midgut  739.6 Larval Hindgut  741.4 Larval Malpighian Tubules  (1196.7) Larval Fat Body    438.8 Larval Salivary Gland  723.2 Larval Trachea  543.15 Larval Carcass  (1282.3) Adult Head    296.1 Adult Eye    300.45 Adult Brain    305.3 Adult Thoracic-Abdominal Ganglion    311.5 Adult Crop  595.8 Adult Midgut    428.9 Adult Hindgut    417.1 Adult Malpighian Tubules    421.8 Adult Fat Body  631.3 Adult Salivary Gland    364.1 Adult Heart  784.25 Adult VirginFemale Spermatheca    387.6 Adult InseminatedFemale Spermatheca    384.2 Adult Ovary  676.7 Adult Testis    426.3 Adult Male Accessory Gland    305 Adult Carcass  528.2 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    650.625 Larval Midgut    739.6 Larval Hindgut    741.4 Larval Malpighian Tubules  1196.7 Larval Fat Body    438.8 Larval Salivary Gland    723.2 Larval Trachea    543.15 Larval Carcass  1282.3 Adult Head    296.1 Adult Eye    300.45 Adult Brain    305.3 Adult Thoracic-Abdominal Ganglion    311.5 Adult Crop    595.8 Adult Midgut    428.9 Adult Hindgut    417.1 Adult Malpighian Tubules    421.8 Adult Fat Body    631.3 Adult Salivary Gland    364.1 Adult Heart    784.25 Adult VirginFemale Spermatheca    387.6 Adult InseminatedFemale Spermatheca    384.2 Adult Ovary    676.7 Adult Testis    426.3 Adult Male Accessory Gland    305 Adult Carcass    528.2 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    650.625 Larval Midgut    739.6 Larval Hindgut    741.4 Larval Malpighian Tubules    1196.7 Larval Fat Body    438.8 Larval Salivary Gland    723.2 Larval Trachea    543.15 Larval Carcass    1282.3 Adult Head    296.1 Adult Eye    300.45 Adult Brain    305.3 Adult Thoracic-Abdominal Ganglion    311.5 Adult Crop    595.8 Adult Midgut    428.9 Adult Hindgut    417.1 Adult Malpighian Tubules    421.8 Adult Fat Body    631.3 Adult Salivary Gland    364.1 Adult Heart    784.25 Adult VirginFemale Spermatheca    387.6 Adult InseminatedFemale Spermatheca    384.2 Adult Ovary    676.7 Adult Testis    426.3 Adult Male Accessory Gland    305 Adult Carcass    528.2 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    296.1   NA  Eye    300.45   NA  Brain    305.3   650.625  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    311.5   NA  Crop    595.8   739.6  Midgut    428.9   741.4  Hindgut    417.1   1196.7  Malpighian Tubules    421.8   438.8  Fat Body    631.3   723.2  Salivary Gland    364.1   NA  Heart    784.25   543.15  Trachea    NA   NA  VirginFemale Spermatheca    387.6   NA  InseminatedFemale Spermatheca    384.2   NA  Ovary    676.7   NA  Testis    426.3   NA  Male Accessory Gland    305   1282.3  Carcass    528.2 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    192 central nervous system, larvae L3    236 central nervous system, pupae P8    102 head, virgin 1-day female    76 head, virgin 4-day female    74 head, virgin 20-day female    38 head, mated 1-day female    108 head, mated 4-day female    81 head, mated 20-day female    44 head, mated 1-day male    80 head, mated 4-day male    97 head, mated 20-day male    102 salivary gland, larvae L3 wandering    69 salivary gland, white prepupae    105 digestive system, larvae L3 wandering    333 digestive system, 1-day adult    69 digestive system, 4-day adult    102 digestive system, 20-day adult    65 fat body, larvae L3 wandering    140 fat body, white prepupae    179 fat body, pupae P8    136 carcass, larvae L3 wandering    456 carcass, 1-day adult    98 carcass, 4-day adult    111 carcass, 20-day adult    100 ovary, virgin 4-day female    163 ovary, mated 4-day female    336 testis, mated 4-day male    281 accessory gland, mated 4-day male    64 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (192) central nervous system, larvae L3  (236) central nervous system, pupae P8  (102) head, virgin 1-day female  (76) head, virgin 4-day female  (74) head, virgin 20-day female  (38) head, mated 1-day female  (108) head, mated 4-day female  (81) head, mated 20-day female  (44) head, mated 1-day male  (80) head, mated 4-day male  (97) head, mated 20-day male  (102) salivary gland, larvae L3 wandering  (69) salivary gland, white prepupae  (105) digestive system, larvae L3 wandering  (333) digestive system, 1-day adult  (69) digestive system, 4-day adult  (102) digestive system, 20-day adult  (65) fat body, larvae L3 wandering  (140) fat body, white prepupae  (179) fat body, pupae P8  (136) carcass, larvae L3 wandering  (456) carcass, 1-day adult  (98) carcass, 4-day adult  (111) carcass, 20-day adult  (100) ovary, virgin 4-day female  (163) ovary, mated 4-day female  (336) testis, mated 4-day male  (281) accessory gland, mated 4-day male  (64) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (192) central nervous system, larvae L3  (236) central nervous system, pupae P8  102 head, virgin 1-day female    76 head, virgin 4-day female    74 head, virgin 20-day female    38 head, mated 1-day female  (108) head, mated 4-day female    81 head, mated 20-day female    44 head, mated 1-day male    80 head, mated 4-day male    97 head, mated 20-day male  102 salivary gland, larvae L3 wandering    69 salivary gland, white prepupae  105 digestive system, larvae L3 wandering  (333) digestive system, 1-day adult    69 digestive system, 4-day adult  102 digestive system, 20-day adult    65 fat body, larvae L3 wandering  (140) fat body, white prepupae  (179) fat body, pupae P8  (136) carcass, larvae L3 wandering  (456) carcass, 1-day adult    98 carcass, 4-day adult  (111) carcass, 20-day adult    100 ovary, virgin 4-day female  (163) ovary, mated 4-day female  (336) testis, mated 4-day male  (281) accessory gland, mated 4-day male    64 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    192 central nervous system, larvae L3    236 central nervous system, pupae P8    102 head, virgin 1-day female    76 head, virgin 4-day female    74 head, virgin 20-day female    38 head, mated 1-day female    108 head, mated 4-day female    81 head, mated 20-day female    44 head, mated 1-day male    80 head, mated 4-day male    97 head, mated 20-day male    102 salivary gland, larvae L3 wandering    69 salivary gland, white prepupae    105 digestive system, larvae L3 wandering    333 digestive system, 1-day adult    69 digestive system, 4-day adult    102 digestive system, 20-day adult    65 fat body, larvae L3 wandering    140 fat body, white prepupae    179 fat body, pupae P8    136 carcass, larvae L3 wandering    456 carcass, 1-day adult    98 carcass, 4-day adult    111 carcass, 20-day adult    100 ovary, virgin 4-day female    163 ovary, mated 4-day female    336 testis, mated 4-day male    281 accessory gland, mated 4-day male    64 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    192 central nervous system, larvae L3    236 central nervous system, pupae P8    102 head, virgin 1-day female    76 head, virgin 4-day female    74 head, virgin 20-day female    38 head, mated 1-day female    108 head, mated 4-day female    81 head, mated 20-day female    44 head, mated 1-day male    80 head, mated 4-day male    97 head, mated 20-day male    102 salivary gland, larvae L3 wandering    69 salivary gland, white prepupae    105 digestive system, larvae L3 wandering    333 digestive system, 1-day adult    69 digestive system, 4-day adult    102 digestive system, 20-day adult    65 fat body, larvae L3 wandering    140 fat body, white prepupae    179 fat body, pupae P8    136 carcass, larvae L3 wandering    456 carcass, 1-day adult    98 carcass, 4-day adult    111 carcass, 20-day adult    100 ovary, virgin 4-day female    163 ovary, mated 4-day female    336 testis, mated 4-day male    281 accessory gland, mated 4-day male    64 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (192) central nervous system, larvae L3  (236) central nervous system, pupae P8  (102) head, virgin 1-day female  (76) head, virgin 4-day female  (74) head, virgin 20-day female  (38) head, mated 1-day female  (108) head, mated 4-day female  (81) head, mated 20-day female  (44) head, mated 1-day male  (80) head, mated 4-day male  (97) head, mated 20-day male  (102) salivary gland, larvae L3 wandering  (69) salivary gland, white prepupae  (105) digestive system, larvae L3 wandering  (333) digestive system, 1-day adult  (69) digestive system, 4-day adult  (102) digestive system, 20-day adult  (65) fat body, larvae L3 wandering  (140) fat body, white prepupae  (179) fat body, pupae P8  (136) carcass, larvae L3 wandering  (456) carcass, 1-day adult  (98) carcass, 4-day adult  (111) carcass, 20-day adult  (100) ovary, virgin 4-day female  (163) ovary, mated 4-day female  (336) testis, mated 4-day male  (281) accessory gland, mated 4-day male  (64) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (192) central nervous system, larvae L3  (236) central nervous system, pupae P8  102 head, virgin 1-day female    76 head, virgin 4-day female    74 head, virgin 20-day female    38 head, mated 1-day female  108 head, mated 4-day female    81 head, mated 20-day female    44 head, mated 1-day male    80 head, mated 4-day male    97 head, mated 20-day male  102 salivary gland, larvae L3 wandering    69 salivary gland, white prepupae  105 digestive system, larvae L3 wandering  (333) digestive system, 1-day adult    69 digestive system, 4-day adult  102 digestive system, 20-day adult    65 fat body, larvae L3 wandering  140 fat body, white prepupae  (179) fat body, pupae P8  136 carcass, larvae L3 wandering  (456) carcass, 1-day adult    98 carcass, 4-day adult  111 carcass, 20-day adult    100 ovary, virgin 4-day female  (163) ovary, mated 4-day female  (336) testis, mated 4-day male  (281) accessory gland, mated 4-day male    64 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    192 central nervous system, larvae L3    236 central nervous system, pupae P8    102 head, virgin 1-day female    76 head, virgin 4-day female    74 head, virgin 20-day female    38 head, mated 1-day female    108 head, mated 4-day female    81 head, mated 20-day female    44 head, mated 1-day male    80 head, mated 4-day male    97 head, mated 20-day male    102 salivary gland, larvae L3 wandering    69 salivary gland, white prepupae    105 digestive system, larvae L3 wandering    333 digestive system, 1-day adult    69 digestive system, 4-day adult    102 digestive system, 20-day adult    65 fat body, larvae L3 wandering    140 fat body, white prepupae    179 fat body, pupae P8    136 carcass, larvae L3 wandering    456 carcass, 1-day adult    98 carcass, 4-day adult    111 carcass, 20-day adult    100 ovary, virgin 4-day female    163 ovary, mated 4-day female    336 testis, mated 4-day male    281 accessory gland, mated 4-day male    64 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of moderately high expression. Peak expression observed within 00-06 hour embryonic stages, during late larval stages, at stages throughout the pupal period, in adult female stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    230 embryo 02-04hr    54 embryo 04-06hr    101 embryo 06-08hr    114 embryo 08-10hr    94 embryo 10-12hr    97 embryo 12-14hr    56 embryo 14-16hr    59 embryo 16-18hr    40 embryo 18-20hr    31 embryo 20-22hr    28 embryo 22-24hr    27 larva L1    59 larva L2    103 larva L3 12hr old    76 larva L3 puffstage 1-2    108 larva L3 puffstage 3-6    190 larva L3 puffstage 7-9    294 white prepupae new    323 white prepupae 12hr    143 white prepupae 24hr    152 pupae 2d postWPP    101 pupae 3d postWPP    76 pupae 4d postWPP    141 adult male 01day    97 adult male 05day    96 adult male 30day    106 adult female 01day    136 adult female 05day    155 adult female 30day    157 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (230) embryo 02-04hr  (54) embryo 04-06hr  (101) embryo 06-08hr  (114) embryo 08-10hr  (94) embryo 10-12hr  (97) embryo 12-14hr  (56) embryo 14-16hr  (59) embryo 16-18hr  (40) embryo 18-20hr  (31) embryo 20-22hr  (28) embryo 22-24hr  (27) larva L1  (59) larva L2  (103) larva L3 12hr old  (76) larva L3 puffstage 1-2  (108) larva L3 puffstage 3-6  (190) larva L3 puffstage 7-9  (294) white prepupae new  (323) white prepupae 12hr  (143) white prepupae 24hr  (152) pupae 2d postWPP  (101) pupae 3d postWPP  (76) pupae 4d postWPP  (141) adult male 01day  (97) adult male 05day  (96) adult male 30day  (106) adult female 01day  (136) adult female 05day  (155) adult female 30day  (157) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr  (230) embryo 02-04hr    54 embryo 04-06hr  101 embryo 06-08hr  (114) embryo 08-10hr    94 embryo 10-12hr    97 embryo 12-14hr    56 embryo 14-16hr    59 embryo 16-18hr    40 embryo 18-20hr    31 embryo 20-22hr    28 embryo 22-24hr    27 larva L1    59 larva L2  103 larva L3 12hr old    76 larva L3 puffstage 1-2  (108) larva L3 puffstage 3-6  (190) larva L3 puffstage 7-9  (294) white prepupae new  (323) white prepupae 12hr  (143) white prepupae 24hr  (152) pupae 2d postWPP  101 pupae 3d postWPP    76 pupae 4d postWPP  (141) adult male 01day    97 adult male 05day    96 adult male 30day  106 adult female 01day  (136) adult female 05day  (155) adult female 30day  (157) Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    230 embryo 02-04hr    54 embryo 04-06hr    101 embryo 06-08hr    114 embryo 08-10hr    94 embryo 10-12hr    97 embryo 12-14hr    56 embryo 14-16hr    59 embryo 16-18hr    40 embryo 18-20hr    31 embryo 20-22hr    28 embryo 22-24hr    27 larva L1    59 larva L2    103 larva L3 12hr old    76 larva L3 puffstage 1-2    108 larva L3 puffstage 3-6    190 larva L3 puffstage 7-9    294 white prepupae new    323 white prepupae 12hr    143 white prepupae 24hr    152 pupae 2d postWPP    101 pupae 3d postWPP    76 pupae 4d postWPP    141 adult male 01day    97 adult male 05day    96 adult male 30day    106 adult female 01day    136 adult female 05day    155 adult female 30day    157 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    230 embryo 02-04hr    54 embryo 04-06hr    101 embryo 06-08hr    114 embryo 08-10hr    94 embryo 10-12hr    97 embryo 12-14hr    56 embryo 14-16hr    59 embryo 16-18hr    40 embryo 18-20hr    31 embryo 20-22hr    28 embryo 22-24hr    27 larva L1    59 larva L2    103 larva L3 12hr old    76 larva L3 puffstage 1-2    108 larva L3 puffstage 3-6    190 larva L3 puffstage 7-9    294 white prepupae new    323 white prepupae 12hr    143 white prepupae 24hr    152 pupae 2d postWPP    101 pupae 3d postWPP    76 pupae 4d postWPP    141 adult male 01day    97 adult male 05day    96 adult male 30day    106 adult female 01day    136 adult female 05day    155 adult female 30day    157 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (230) embryo 02-04hr  (54) embryo 04-06hr  (101) embryo 06-08hr  (114) embryo 08-10hr  (94) embryo 10-12hr  (97) embryo 12-14hr  (56) embryo 14-16hr  (59) embryo 16-18hr  (40) embryo 18-20hr  31 embryo 20-22hr  28 embryo 22-24hr  27 larva L1  (59) larva L2  (103) larva L3 12hr old  (76) larva L3 puffstage 1-2  (108) larva L3 puffstage 3-6  (190) larva L3 puffstage 7-9  (294) white prepupae new  (323) white prepupae 12hr  (143) white prepupae 24hr  (152) pupae 2d postWPP  (101) pupae 3d postWPP  (76) pupae 4d postWPP  (141) adult male 01day  (97) adult male 05day  (96) adult male 30day  (106) adult female 01day  (136) adult female 05day  (155) adult female 30day  (157) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr  (230) embryo 02-04hr    54 embryo 04-06hr  101 embryo 06-08hr  114 embryo 08-10hr    94 embryo 10-12hr    97 embryo 12-14hr    56 embryo 14-16hr    59 embryo 16-18hr    40 embryo 18-20hr    31 embryo 20-22hr    28 embryo 22-24hr    27 larva L1    59 larva L2  103 larva L3 12hr old    76 larva L3 puffstage 1-2  108 larva L3 puffstage 3-6  (190) larva L3 puffstage 7-9  (294) white prepupae new  (323) white prepupae 12hr  143 white prepupae 24hr  (152) pupae 2d postWPP  101 pupae 3d postWPP    76 pupae 4d postWPP  141 adult male 01day    97 adult male 05day    96 adult male 30day  106 adult female 01day  136 adult female 05day  (155) adult female 30day  (157) Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    230 embryo 02-04hr    54 embryo 04-06hr    101 embryo 06-08hr    114 embryo 08-10hr    94 embryo 10-12hr    97 embryo 12-14hr    56 embryo 14-16hr    59 embryo 16-18hr    40 embryo 18-20hr    31 embryo 20-22hr    28 embryo 22-24hr    27 larva L1    59 larva L2    103 larva L3 12hr old    76 larva L3 puffstage 1-2    108 larva L3 puffstage 3-6    190 larva L3 puffstage 7-9    294 white prepupae new    323 white prepupae 12hr    143 white prepupae 24hr    152 pupae 2d postWPP    101 pupae 3d postWPP    76 pupae 4d postWPP    141 adult male 01day    97 adult male 05day    96 adult male 30day    106 adult female 01day    136 adult female 05day    155 adult female 30day    157 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    182 Schneider line 2 Sg4    207 embryonic 1182-4H    235 embryonic GM2    61 embryonic Kc167    102 embryonic S1    133 embryonic S3    122 leg disc CME L1    337 wing disc CME-W2    127 wing disc ML-DmD8    134 wing disc ML-DmD9    206 wing disc ML-DmD16-c3    452 wing disc ML-DmD21    388 wing disc ML-DmD32    201 haltere disc ML-DmD17-c3    158 eye-antennal disc ML-DmD11    147 antennal disc ML-DmD20-c5    253 mixed discs ML-DmD4-c1    301 CNS ML-DmBG1-c1    327 CNS ML-DmBG2-c2    233 tumorous blood cells mbn2    136 ovary fGS/OSS    367 ovary OSC    405 ovary OSS    267 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (182) Schneider line 2 Sg4  (207) embryonic 1182-4H  (235) embryonic GM2  (61) embryonic Kc167  (102) embryonic S1  (133) embryonic S3  (122) leg disc CME L1  (337) wing disc CME-W2  (127) wing disc ML-DmD8  (134) wing disc ML-DmD9  (206) wing disc ML-DmD16-c3  (452) wing disc ML-DmD21  (388) wing disc ML-DmD32  (201) haltere disc ML-DmD17-c3  (158) eye-antennal disc ML-DmD11  (147) antennal disc ML-DmD20-c5  (253) mixed discs ML-DmD4-c1  (301) CNS ML-DmBG1-c1  (327) CNS ML-DmBG2-c2  (233) tumorous blood cells mbn2  (136) ovary fGS/OSS  (367) ovary OSC  (405) ovary OSS  (267) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+  (182) Schneider line 2 Sg4  (207) embryonic 1182-4H  (235) embryonic GM2    61 embryonic Kc167  102 embryonic S1  (133) embryonic S3  (122) leg disc CME L1  (337) wing disc CME-W2  (127) wing disc ML-DmD8  (134) wing disc ML-DmD9  (206) wing disc ML-DmD16-c3  (452) wing disc ML-DmD21  (388) wing disc ML-DmD32  (201) haltere disc ML-DmD17-c3  (158) eye-antennal disc ML-DmD11  (147) antennal disc ML-DmD20-c5  (253) mixed discs ML-DmD4-c1  (301) CNS ML-DmBG1-c1  (327) CNS ML-DmBG2-c2  (233) tumorous blood cells mbn2  (136) ovary fGS/OSS  (367) ovary OSC  (405) ovary OSS  (267) Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    182 Schneider line 2 Sg4    207 embryonic 1182-4H    235 embryonic GM2    61 embryonic Kc167    102 embryonic S1    133 embryonic S3    122 leg disc CME L1    337 wing disc CME-W2    127 wing disc ML-DmD8    134 wing disc ML-DmD9    206 wing disc ML-DmD16-c3    452 wing disc ML-DmD21    388 wing disc ML-DmD32    201 haltere disc ML-DmD17-c3    158 eye-antennal disc ML-DmD11    147 antennal disc ML-DmD20-c5    253 mixed discs ML-DmD4-c1    301 CNS ML-DmBG1-c1    327 CNS ML-DmBG2-c2    233 tumorous blood cells mbn2    136 ovary fGS/OSS    367 ovary OSC    405 ovary OSS    267 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    182 Schneider line 2 Sg4    207 embryonic 1182-4H    235 embryonic GM2    61 embryonic Kc167    102 embryonic S1    133 embryonic S3    122 leg disc CME L1    337 wing disc CME-W2    127 wing disc ML-DmD8    134 wing disc ML-DmD9    206 wing disc ML-DmD16-c3    452 wing disc ML-DmD21    388 wing disc ML-DmD32    201 haltere disc ML-DmD17-c3    158 eye-antennal disc ML-DmD11    147 antennal disc ML-DmD20-c5    253 mixed discs ML-DmD4-c1    301 CNS ML-DmBG1-c1    327 CNS ML-DmBG2-c2    233 tumorous blood cells mbn2    136 ovary fGS/OSS    367 ovary OSC    405 ovary OSS    267 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (182) Schneider line 2 Sg4  (207) embryonic 1182-4H  (235) embryonic GM2  (61) embryonic Kc167  (102) embryonic S1  (133) embryonic S3  (122) leg disc CME L1  (337) wing disc CME-W2  (127) wing disc ML-DmD8  (134) wing disc ML-DmD9  (206) wing disc ML-DmD16-c3  (452) wing disc ML-DmD21  (388) wing disc ML-DmD32  (201) haltere disc ML-DmD17-c3  (158) eye-antennal disc ML-DmD11  (147) antennal disc ML-DmD20-c5  (253) mixed discs ML-DmD4-c1  (301) CNS ML-DmBG1-c1  (327) CNS ML-DmBG2-c2  (233) tumorous blood cells mbn2  (136) ovary fGS/OSS  (367) ovary OSC  (405) ovary OSS  (267) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+  (182) Schneider line 2 Sg4  (207) embryonic 1182-4H  (235) embryonic GM2    61 embryonic Kc167  102 embryonic S1  133 embryonic S3  122 leg disc CME L1  (337) wing disc CME-W2  127 wing disc ML-DmD8  134 wing disc ML-DmD9  (206) wing disc ML-DmD16-c3  (452) wing disc ML-DmD21  (388) wing disc ML-DmD32  (201) haltere disc ML-DmD17-c3  (158) eye-antennal disc ML-DmD11  (147) antennal disc ML-DmD20-c5  (253) mixed discs ML-DmD4-c1  (301) CNS ML-DmBG1-c1  (327) CNS ML-DmBG2-c2  (233) tumorous blood cells mbn2  136 ovary fGS/OSS  (367) ovary OSC  (405) ovary OSS  (267) Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    182 Schneider line 2 Sg4    207 embryonic 1182-4H    235 embryonic GM2    61 embryonic Kc167    102 embryonic S1    133 embryonic S3    122 leg disc CME L1    337 wing disc CME-W2    127 wing disc ML-DmD8    134 wing disc ML-DmD9    206 wing disc ML-DmD16-c3    452 wing disc ML-DmD21    388 wing disc ML-DmD32    201 haltere disc ML-DmD17-c3    158 eye-antennal disc ML-DmD11    147 antennal disc ML-DmD20-c5    253 mixed discs ML-DmD4-c1    301 CNS ML-DmBG1-c1    327 CNS ML-DmBG2-c2    233 tumorous blood cells mbn2    136 ovary fGS/OSS    367 ovary OSC    405 ovary OSS    267 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    448 cold shock, 4-day adult    333 heat shock, 4-day adult    138 Cadmium 50 mM 6 hrs, larvae L3    118 Cadmium 50 mM 12 hrs, larvae L3    162 Cadmium 50 mM 48 hrs, 4-day adult    168 Cadmium 100 mM 48 hrs, 4-day adult    275 Copper 0.5 mM 12 hrs, larvae L3    531 Copper 15 mM 48 hrs, 4-day adult    189 Zinc 5 mM 12 hrs, larvae L3    323 Zinc 4.5 mM 48 hrs, 4-day adult    220 Ethanol 2.5% 3 hrs, larvae L3    171 Ethanol 5% 3 hrs, larvae L3    84 Ethanol 10% 3 hrs, larvae L3    102 Caffeine 1.5 mg/ml 4 hrs, larvae L3    99 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    255 Caffeine 25 mg/ml 48 hrs, 4-day adult    433 Paraquat 5 mM 48 hrs, 4-day adult    219 Paraquat 10 mM 48 hrs, 4-day adult    188 Rotenone 2 μg 12 hrs, larvae L3    98 Rotenone 8 μg 12 hrs, larvae L3    91 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  (448) cold shock, 4-day adult  (333) heat shock, 4-day adult  (138) Cadmium 50 mM 6 hrs, larvae L3  (118) Cadmium 50 mM 12 hrs, larvae L3  (162) Cadmium 50 mM 48 hrs, 4-day adult  (168) Cadmium 100 mM 48 hrs, 4-day adult  (275) Copper 0.5 mM 12 hrs, larvae L3  (531) Copper 15 mM 48 hrs, 4-day adult  (189) Zinc 5 mM 12 hrs, larvae L3  (323) Zinc 4.5 mM 48 hrs, 4-day adult  (220) Ethanol 2.5% 3 hrs, larvae L3  (171) Ethanol 5% 3 hrs, larvae L3  (84) Ethanol 10% 3 hrs, larvae L3  (102) Caffeine 1.5 mg/ml 4 hrs, larvae L3  (99) Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (255) Caffeine 25 mg/ml 48 hrs, 4-day adult  (433) Paraquat 5 mM 48 hrs, 4-day adult  (219) Paraquat 10 mM 48 hrs, 4-day adult  (188) Rotenone 2 μg 12 hrs, larvae L3  (98) Rotenone 8 μg 12 hrs, larvae L3  (91) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult  (448) cold shock, 4-day adult  (333) heat shock, 4-day adult  (138) Cadmium 50 mM 6 hrs, larvae L3  (118) Cadmium 50 mM 12 hrs, larvae L3  (162) Cadmium 50 mM 48 hrs, 4-day adult  (168) Cadmium 100 mM 48 hrs, 4-day adult  (275) Copper 0.5 mM 12 hrs, larvae L3  (531) Copper 15 mM 48 hrs, 4-day adult  (189) Zinc 5 mM 12 hrs, larvae L3  (323) Zinc 4.5 mM 48 hrs, 4-day adult  (220) Ethanol 2.5% 3 hrs, larvae L3  (171) Ethanol 5% 3 hrs, larvae L3    84 Ethanol 10% 3 hrs, larvae L3  102 Caffeine 1.5 mg/ml 4 hrs, larvae L3    99 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (255) Caffeine 25 mg/ml 48 hrs, 4-day adult  (433) Paraquat 5 mM 48 hrs, 4-day adult  (219) Paraquat 10 mM 48 hrs, 4-day adult  (188) Rotenone 2 μg 12 hrs, larvae L3    98 Rotenone 8 μg 12 hrs, larvae L3    91 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    448 cold shock, 4-day adult    333 heat shock, 4-day adult    138 Cadmium 50 mM 6 hrs, larvae L3    118 Cadmium 50 mM 12 hrs, larvae L3    162 Cadmium 50 mM 48 hrs, 4-day adult    168 Cadmium 100 mM 48 hrs, 4-day adult    275 Copper 0.5 mM 12 hrs, larvae L3    531 Copper 15 mM 48 hrs, 4-day adult    189 Zinc 5 mM 12 hrs, larvae L3    323 Zinc 4.5 mM 48 hrs, 4-day adult    220 Ethanol 2.5% 3 hrs, larvae L3    171 Ethanol 5% 3 hrs, larvae L3    84 Ethanol 10% 3 hrs, larvae L3    102 Caffeine 1.5 mg/ml 4 hrs, larvae L3    99 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    255 Caffeine 25 mg/ml 48 hrs, 4-day adult    433 Paraquat 5 mM 48 hrs, 4-day adult    219 Paraquat 10 mM 48 hrs, 4-day adult    188 Rotenone 2 μg 12 hrs, larvae L3    98 Rotenone 8 μg 12 hrs, larvae L3    91 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    448 cold shock, 4-day adult    333 heat shock, 4-day adult    138 Cadmium 50 mM 6 hrs, larvae L3    118 Cadmium 50 mM 12 hrs, larvae L3    162 Cadmium 50 mM 48 hrs, 4-day adult    168 Cadmium 100 mM 48 hrs, 4-day adult    275 Copper 0.5 mM 12 hrs, larvae L3    531 Copper 15 mM 48 hrs, 4-day adult    189 Zinc 5 mM 12 hrs, larvae L3    323 Zinc 4.5 mM 48 hrs, 4-day adult    220 Ethanol 2.5% 3 hrs, larvae L3    171 Ethanol 5% 3 hrs, larvae L3    84 Ethanol 10% 3 hrs, larvae L3    102 Caffeine 1.5 mg/ml 4 hrs, larvae L3    99 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    255 Caffeine 25 mg/ml 48 hrs, 4-day adult    433 Paraquat 5 mM 48 hrs, 4-day adult    219 Paraquat 10 mM 48 hrs, 4-day adult    188 Rotenone 2 μg 12 hrs, larvae L3    98 Rotenone 8 μg 12 hrs, larvae L3    91 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  (448) cold shock, 4-day adult  (333) heat shock, 4-day adult  (138) Cadmium 50 mM 6 hrs, larvae L3  (118) Cadmium 50 mM 12 hrs, larvae L3  (162) Cadmium 50 mM 48 hrs, 4-day adult  (168) Cadmium 100 mM 48 hrs, 4-day adult  (275) Copper 0.5 mM 12 hrs, larvae L3  (531) Copper 15 mM 48 hrs, 4-day adult  (189) Zinc 5 mM 12 hrs, larvae L3  (323) Zinc 4.5 mM 48 hrs, 4-day adult  (220) Ethanol 2.5% 3 hrs, larvae L3  (171) Ethanol 5% 3 hrs, larvae L3  (84) Ethanol 10% 3 hrs, larvae L3  (102) Caffeine 1.5 mg/ml 4 hrs, larvae L3  (99) Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (255) Caffeine 25 mg/ml 48 hrs, 4-day adult  (433) Paraquat 5 mM 48 hrs, 4-day adult  (219) Paraquat 10 mM 48 hrs, 4-day adult  (188) Rotenone 2 μg 12 hrs, larvae L3  (98) Rotenone 8 μg 12 hrs, larvae L3  (91) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult  (448) cold shock, 4-day adult  (333) heat shock, 4-day adult  138 Cadmium 50 mM 6 hrs, larvae L3  118 Cadmium 50 mM 12 hrs, larvae L3  (162) Cadmium 50 mM 48 hrs, 4-day adult  (168) Cadmium 100 mM 48 hrs, 4-day adult  (275) Copper 0.5 mM 12 hrs, larvae L3  (531) Copper 15 mM 48 hrs, 4-day adult  (189) Zinc 5 mM 12 hrs, larvae L3  (323) Zinc 4.5 mM 48 hrs, 4-day adult  (220) Ethanol 2.5% 3 hrs, larvae L3  (171) Ethanol 5% 3 hrs, larvae L3    84 Ethanol 10% 3 hrs, larvae L3  102 Caffeine 1.5 mg/ml 4 hrs, larvae L3    99 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (255) Caffeine 25 mg/ml 48 hrs, 4-day adult  (433) Paraquat 5 mM 48 hrs, 4-day adult  (219) Paraquat 10 mM 48 hrs, 4-day adult  (188) Rotenone 2 μg 12 hrs, larvae L3    98 Rotenone 8 μg 12 hrs, larvae L3    91 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    448 cold shock, 4-day adult    333 heat shock, 4-day adult    138 Cadmium 50 mM 6 hrs, larvae L3    118 Cadmium 50 mM 12 hrs, larvae L3    162 Cadmium 50 mM 48 hrs, 4-day adult    168 Cadmium 100 mM 48 hrs, 4-day adult    275 Copper 0.5 mM 12 hrs, larvae L3    531 Copper 15 mM 48 hrs, 4-day adult    189 Zinc 5 mM 12 hrs, larvae L3    323 Zinc 4.5 mM 48 hrs, 4-day adult    220 Ethanol 2.5% 3 hrs, larvae L3    171 Ethanol 5% 3 hrs, larvae L3    84 Ethanol 10% 3 hrs, larvae L3    102 Caffeine 1.5 mg/ml 4 hrs, larvae L3    99 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    255 Caffeine 25 mg/ml 48 hrs, 4-day adult    433 Paraquat 5 mM 48 hrs, 4-day adult    219 Paraquat 10 mM 48 hrs, 4-day adult    188 Rotenone 2 μg 12 hrs, larvae L3    98 Rotenone 8 μg 12 hrs, larvae L3    91 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_02 mE1_20_mRNA_expression_cluster_10
External Data & Images
Linkouts	BDGP expression data • AT07973 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0002787 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG3416-RA FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for Rpn8 Stages(s) 4-6 Stages(s) 7-8 Stages(s) 9-10 Stages(s) 11-12 Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal Rpn8GD10960 lethal, with Scer\GAL4pnr-MD237 Rpn8GD10960 lethal | larval stage, with Scer\GAL4sqh.PW Rpn8KK101319 lethal | pharate adult stage, with Scer\GAL4elav.PU Rpn8KK101319 lethal | pupal stage, with Scer\GAL4dpp.PU Rpn8GD10960 Rpn8KK101319 lethal | pupal stage, with Scer\GAL4elav.PU Rpn8KK101319 lethal | pupal stage, with Scer\GAL4sqh.PW Rpn8KK101319 lethal | recessive Rpn8k08003 Rpn8k16724 Other Phenotypes Allele decreased cell number | somatic clone Rpn8k08003 neuroanatomy defective | larval stage Rpn8GD10960 neuroanatomy defective | pupal stage Rpn8k08003 neuroanatomy defective | somatic clone Rpn8k08003 visible, with Scer\GAL4bbg-C96 Rpn8GD10960 Rpn8KK101319 Phenotype manifest in Allele dendritic arborizing neuron | P-stage Rpn8k08003 embryonic/larval neuroblast | larval stage Rpn8GD10960 ganglion mother cell | larval stage Rpn8GD10960 mushroom body | somatic clone Rpn8k08003 mushroom body gamma-lobe | somatic clone Rpn8k08003 neuroblast | somatic clone Rpn8k08003 wing, with Scer\GAL4bbg-C96 Rpn8GD10960 Rpn8KK101319
Classical Alleles ( 2 )
For All Classical Alleles Show
Allele of Rpn8 Class Mutagen Stocks Known lesion Rpn8k08003 P-element activity 2 -- Rpn8k16724 P-element activity 1 --
Alleles Carried on Transgenic Constructs ( 6 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Rpn8 Class Mutagen Stocks Known lesion Rpn8GD10960 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Rpn8GL00333 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Rpn8JF01140 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Rpn8KK101319 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Rpn8MtnA.C.T:Avic\GFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes Rpn8MtnA.N.T:Avic\GFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2R)BSC603 (Christensen et al., 2008.9.3) Df(2R)BSC780 (Christensen et al., 2009.5.6) Df(2R)bwVDe2LPxKR (Spradling et al., 1999, Beaton, 1997.12.27) Df(2R)Exel6278 (Ryder, 2004.4.26) In(2LR)Px4 (Spradling et al., 1999, BDGP Project Members, 1994-1999)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data
Insertions
	Type of insertions Name Expression data insertion of enhancer trap P{lacW}l(2)SH0306SH0306 No P{lacW}Rpn8k08003 No P{lacW}Rpn8k16724 No insertion of mobile activating element P{GSV1}C125C125 NA
Gene Ontology: Function, Process & Cellular Component ( 13 unique terms )
Terms Based on Experimental Evidence ( 10 terms )
Molecular Function
CV term Evidence References
contributes_to endopeptidase activity inferred from direct assay (Holzl et al., 2000, Udvardy, 1993)
Biological Process
CV term Evidence References
cell proliferation inferred from mutant phenotype (Goshima et al., 2007) cellular process inferred from mutant phenotype (Boutros et al., 2004) mitotic spindle elongation inferred from mutant phenotype (Goshima et al., 2007) mitotic spindle organization inferred from mutant phenotype (Goshima et al., 2007) neurogenesis inferred from mutant phenotype (Neumüller et al., 2011) proteolysis inferred from direct assay AND inferred from sequence or structural similarity with human PSMD7 AND inferred from sequence or structural similarity with Saccharomyces RPN8 (Holzl et al., 2000)
Cellular Component
CV term Evidence References
dendrite inferred from mutant phenotype (Kuo et al., 2005) proteasome complex inferred from direct assay (Udvardy, 1993) proteasome regulatory particle inferred from direct assay (Sumegi et al., 2003) inferred from direct assay AND inferred from sequence or structural similarity with human PSMD7 (Holzl et al., 2000)
Terms Based on Predictions or Assertions ( 5 terms )
Molecular Function
CV term Evidence References
endopeptidase activity non-traceable author statement (Spradling et al., 1999)
Biological Process
CV term Evidence References
proteasomal ubiquitin-dependent protein catabolic process inferred by curator from GO:0005838 (Sumegi et al., 2003) ubiquitin-dependent protein catabolic process non-traceable author statement (Swiss-Prot Project Members, 1992.5.1)
Cellular Component
CV term Evidence References
proteasome complex non-traceable author statement (Swiss-Prot Project Members, 1992.5.1) proteasome regulatory particle, lid subcomplex inferred from sequence or structural similarity with Saccharomyces RPN8 (Holzl et al., 2000) non-traceable author statement (Spradling et al., 1999)
Sequence Ontology: Class of Gene
	gene_with_stop_codon_read_through (Jungreis et al., 2011) nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
Rpn8 - CG13321 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rpn8 - CG18789 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rpn8 - CG5196 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rpn8 - LanB1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rpn8 - NimB1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rpn8 - Prosα2 experimental knowledge based (Guruharsha et al., 2011) Rpn8 - Prosα3 experimental knowledge based (Guruharsha et al., 2011) Rpn8 - Prosα4 experimental knowledge based (Guruharsha et al., 2011) Rpn8 - Prosα5 experimental knowledge based (Guruharsha et al., 2011) Rpn8 - Prosα6 experimental knowledge based (Guruharsha et al., 2011) Rpn8 - Prosα7 experimental knowledge based (Guruharsha et al., 2011) Rpn8 - Prosβ1 experimental knowledge based (Guruharsha et al., 2011) Rpn8 - Prosβ2 experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) Rpn8 - Prosβ3 experimental knowledge based (Guruharsha et al., 2011) Rpn8 - Prosβ4 experimental knowledge based (Guruharsha et al., 2011) Rpn8 - Prosβ5 experimental knowledge based (Guruharsha et al., 2011) Rpn8 - Prosβ6 experimental knowledge based (Guruharsha et al., 2011) Rpn8 - Prosβ7 experimental knowledge based (Guruharsha et al., 2011) Rpn8 - Rpn1 experimental knowledge based (Guruharsha et al., 2011) Rpn8 - Rpn11 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) Rpn8 - Rpn13 experimental knowledge based (Guruharsha et al., 2011) Rpn8 - Rpn2 experimental knowledge based (Guruharsha et al., 2011) Rpn8 - Rpn3 experimental knowledge based (Guruharsha et al., 2011) Rpn8 - Rpn5 experimental knowledge based (Guruharsha et al., 2011) Rpn8 - Rpn6 experimental knowledge based (Guruharsha et al., 2011) Rpn8 - Rpn7 experimental knowledge based (Guruharsha et al., 2011) Rpn8 - Rpn9 experimental knowledge based (Guruharsha et al., 2011) Rpn8 - Rpt2 experimental knowledge based (Guruharsha et al., 2011) Rpn8 - Rpt3 experimental knowledge based (Guruharsha et al., 2011) Rpn8 - Rpt4 experimental knowledge based (Guruharsha et al., 2011) Rpn8 - Rpt5 experimental knowledge based (Guruharsha et al., 2011) Rpn8 - Rpt6 experimental knowledge based (Guruharsha et al., 2011) Rpn8 - Uch-L5 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rpn8 - eIF3-S8 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rpn8 - pnut anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rpn8 - spi anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Rpn8 allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 63468 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0002787 DroID - A comprehensive database of gene and protein interactions. • FBgn0002787 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 37894
Orthologs
OrthoDB Orthologs (38) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG69ZZ8D Dipteran inclusive ortholog search EOG6VDQ7B Insect inclusive ortholog search EOG6MGR49 Arthropod inclusive ortholog search EOG6QZ62V Metazoa inclusive ortholog search EOG67MDS1
Orthologs in Drosophila Species (EOG69ZZ8D)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Rpn8     Drosophila simulans   Dsim\GD11854 Y   Drosophila sechellia   Dsec\GM11856 Y   Drosophila erecta   Dere\GG22963 Y   Drosophila yakuba   Dyak\GE14400 Y   Drosophila ananassae   Dana\GF13608 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA17437 Y   Drosophila persimilis   Dper\GL20068 Y   Drosophila willistoni   Dwil\GK22207 Y   Drosophila virilis   Dvir\GJ21597 Y   Drosophila mojavensis   Dmoj\GI21325 Y   Drosophila grimshawi   Dgri\GH20991 Y
Orthologs in non-Drosophila Dipterans (EOG6VDQ7B)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL000270   Anopheles gambiae Malaria mosquito  Agam\AGAP012135   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ015055
Orthologs in non-Dipteran Insects (EOG6MGR49)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\LOC408413   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG001600   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH17584   Atta cephalotes Leafcutter ant  Acep\ACEP21446   Camponotus floridanus Florida carpenter ant  Cflo\CFLO15117   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL14470   Linepithema humile Argentine ant  Lhum\LH16556   Pogonomyrmex barbatus Red harvester ant  Pbar\PB24763   Acyrthosiphon pisum Pea aphid  Apim\ACYPI007679   Bombyx mori Silkmoth  Bmor\BGIBMGA005994   Pediculus humanus Human body louse  Phum\PHUM077900   Tribolium castaneum Red flour beetle  Tcas\TC013318
Orthologs in non-Insect Arthropods (EOG6QZ62V)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\matK   Ixodes scapularis Deer tick  Isca\ISCW022113
Orthologs in non-Arthropod Metazoa (EOG67MDS1)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\rpn-8   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_000976gn   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_024140gn   Danio rerio Zebrafish  Drer\psmd7   Xenopus tropicalis Western clawed frog  Xtro\psmd7   Gallus gallus Domestic chicken  Ggal\PSMD7   Mus musculus House mouse  Mmus\Psmd7   Rattus norvegicus Norway rat  Rnor\Psmd7   Homo sapiens Human  Hsap\PSMD7
Human Orthologs (1)
Gene OMIM HGNC Hsap\PSMD7
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD11854 Drosophila sechellia Dsec\GM11856 Drosophila erecta Dere\GG22963 Drosophila yakuba Dyak\GE14400 Drosophila ananassae Dana\GF13608 Drosophila pseudoobscura pseudoobscura Dpse\GA17437 Drosophila persimilis Dper\GL20068 Drosophila willistoni Dwil\GK22207 Drosophila virilis Dvir\GJ21597 Drosophila mojavensis Dmoj\GI21325 Drosophila grimshawi Dgri\GH20991
Stocks & Reagents
Stocks Listed in FlyBase ( 7 )
Bloomington	31567 y1 v1; P{TRiP.JF01140}attP2 35411 y1 sc* v1; P{TRiP.GL00333}attP2 23860 y1 w*; P{neoFRT}42D P{lacW}Rpn8k08003/CyO
Kyoto	103035 y1 w67c23 ; P{lacW}Rpn8k16724 / CyO 111449 yd2 w1118 P{ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1; P{neoFRT}42D P{lacW}Rpn8k08003 /CyO y+
VDRC	v108573 P{KK101319}VIE-260B v26183 w1118 P{GD10960}v26183
Genomic Clones ( 1 )
	BACR02A05 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 203 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	AT07973
Other clones	DPiM_AT07973
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT12201 AT13776 AT15740 AT18377 AT25105 GM02223 GM03721 GM07730 GM09629 GM10795 HL02986 LD08476 LD18147 LD22193 LD36460 LD45989 LP11805 LP14047 LP20261 RE15848 RE21904 RE74124
Other clones	148324 AT21309 bs80g08 EK254226 RP003011790 RP003019984 RP003024073 RP003024555 RP003024582 RP003025823 RP003030016 RP003032235 RP003033717 RP003036681 RP003047764 RP003051224 RP003053334 RP003053843 RP003055486 RP003058315 RP003061110 RP003065643 RP003071667 RP003079729 RP003080701 RP003081817 RP003085629 RP003088096 RP003088369 RP003093222 RP003093847 RP003097641 RP003097980 RP003100915 RP003101406 RP003105015 RP003111475 RP003116226 RP003122234 RP003122388 RP003122427 RP003125918 RP003134929 RP003136621 RP003139934 RP003142736 RP003147833 RP003148924 RP003156437 RP003161040 RP003162496 RP003165308 RP003167702 RP003171658 RP003173188 RP003177526 RP003181990 RP003186067 RP003187955 RP003188361 RP003190521 RP003192348 RP003192797 RP003195932 RP003196789 RP003203234 RP003209549 RP003209715 RP003210192 RP003213652 RP003214133 RP003214326 RP003215590 RP003216346 RP003216808 RP003222698 RP003223892 RP003227495 RP003230231 RP003235288 RP003238909 RP003253384 RP003255212 RP003258785 RP003261572 RP003266825 RP003275469 RP003281015 RP003281667 RP003284539 RP003284619 RP003287279 RP003289827 RP003289979 RP003293157 RP003296885 RP003303079 RP003304800 RP003318352 RP003323945 RP003324092 RP003324721 RP003327027 RP003330671 RP003331732 RP003333309 RP003339798 RP003342553 RP003343247 RP003345778 RP003350420 RP003351965 RP003354416 RP003354650 RP003372387 RP003383161 RP003383613 RP003385261 RP003390280 RP003396832 RP003399875 RP003403987 RP003413682 RP003417362 RP003423360 RP003427662 RP003434942 RP003437036 RP003446886 RP003446923 RP003448756 RP003453630 RP003459314 RP003467359 RP003473654 RP003474942 RP003486926 RP003489590 RP003490159 RP003492622 RP003493456 RP003494759 RP003501091 RP003505716 RP003506602 RP003512406 RP003518235 RP003520406 RP003522569 RP003528968 RP003536719 RP003560082 RP003561035 RP003574258 RP003579058 RP003580615 RP003597063 RP003598052 RP003611774 RP003617057 RP003632157 RP003633373 RP003644277 RP003647144 RP003662005 RP003670990 RP003672833 RP003675456 RP003681291 RP004056578 RP004097222 RP004099792 RP004110389 RP004199480 RP004347044 RP004413781 RP004419795 RP004445540 RP004529310
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0002787 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 37894
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: Mov34 CG3416 (Wang, 1999.11) Source for identity of: Rpn8 Mov34 (FlyBase, 2009-)
Source for database merge of	Source for merge of: Mov34 l(2)k08003 (Spradling et al., 1999)
Additional comments	The nomenclature of genes encoding subunits of the 26S proteasome of D. melanogaster have been standardized according to FBrf0215459. These symbols/names largely follow those used already in FlyBase, and largely mirror fly community usage. HOWEVER, note that at least one other nomenclature system exists that is followed by the HUGO Gene Nomenclature Committee (HGNC), for example, with the unfortunate result that several D. melanogaster genes have shared synonyms. (FlyBase, 2009-)
Other Comments
	RNAi screen using dsRNA made from templates generated with primers directed against this gene results in the formation of short, monastral bipolar spindles and severe proliferation defects when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA. (Goshima et al., 2007) RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R+ cells. (Boutros et al., 2004) RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R+ cells. (Boutros et al., 2004) The 26S proteolytic complex, a multiprotein complex containing many subunits (including those encoded by Pros26.4, Pros54 and Mov34), can be assembled from two smaller multiprotein complexes, the μ particle and the 20S proteasome, by a process that requires ATP. (Udvardy, 1993) Mov34 has been cloned and analysed. (Gridley et al., 1990)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_079121 NM_001274238 NM_001274239 RefSeq (Proteins) • NP_523845 NP_001261167 NP_001261168 DDBJ / EMBL / GenBank • AA697688 AAA28695 AAF47199 AAL90021 AGB93697 AGB93698 AQ025851 AY089283 BF505696 M64643 Entrez Gene - A searchable database of RefSeq genes. • 37894 UniProtKB/Swiss-Prot • P26270
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Rpn11/EIF3F C-terminal domain (IPR024969) JAB1/Mov34/MPN/PAD-1 (IPR000555) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • AT07973
Linkouts
	BioGRID - A database of protein and genetic interactions • 63468 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0002787 DroID - A comprehensive database of gene and protein interactions. • FBgn0002787 DRSC - Results from RNAi screens. • FBgn0002787 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0002787 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG3416-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0002787 FlyReactome - A curated knowledgebase of Drosophila melanogaster pathways • 99116 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 37894 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 37894 modMine - Data generated by the modENCODE project. • FBgn0002787
Synonyms & Secondary IDs ( 10 )
Reported As
Symbol Synonym	CG3416 (Nakahara et al., 2005, Goshima et al., 2007, Molnar et al., 2006, Zhang et al., 2012, Lebo et al., 2009, Barbash and Lorigan, 2007, Zhang et al., 2012, Keleman et al., 2009.8.5, Ma et al., 2002, Gandhi et al., 2007, Ni et al., 2008, Tsou et al., 2012, Ni et al., 2011.7.19, Cammarato et al., 2011, Lundgren et al., 2005, Belote and Zhong, 2009) l(2)k08003 (Beaton, 1997.12.27, BDGP Project Members, 1994-1999, ) Mov34 (Jungreis et al., 2011, Zhang et al., 2012, Lebo et al., 2009, Zhang et al., 2012, Ma et al., 2002, Lundgren et al., 2005, Tsou et al., 2012, Valakh et al., 2012, Stuart et al., 2007, Hoopfer et al., 2008, Goshima et al., 2007, Du et al., 2011, Molnar et al., 2006, Christensen et al., 2008.9.3, Barbash and Lorigan, 2007, Christensen et al., 2009.5.6, Neumüller et al., 2011) p39B (Sumegi et al., 2003, Kurucz et al., 2002, Holzl et al., 2000, Ma et al., 2002) PRSC_DROME (Aravind and Ponting, 1998) RPN8 (Gemmill et al., 2005) Rpn8 (Lee et al., 2010, Nickell et al., 2009, Ma et al., 2002, Lundgren et al., 2005, Belote and Zhong, 2009) S12 (Lundgren et al., 2005) unnamed (Udvardy, 1993)
Name Synonym	Mov34 (Cammarato et al., 2011) Regulatory particle non-ATPase 8
Secondary FlyBase IDs
	FBgn0022065
References ( 60 )
Generate a list of	All references relating to this gene ( 60 )
List References by type	Research paper  ( 28 ) Supplementary material  ( 13 ) Review  ( 1 ) Personal communication to FlyBase  ( 11 ) FlyBase analysis  ( 2 ) Curated genome annotation  ( 3 ) Protein sequence record  ( 1 ) Website  ( 1 )
Recent research papers ( 6 )
	Tsou et al., 2012, PLoS ONE 7(8): e43112 Systematic Analysis of the Physiological Importance of Deubiquitinating Enzymes. [FBrf0219256] Valakh et al., 2012, Dev. Biol. 366(2): 163--171 A large-scale RNAi screen identifies functional classes of genes shaping synaptic development and maintenance. [FBrf0218359] Zhang et al., 2012, G3 (Bethesda) 2(12): 1563--1575 A Targeted In Vivo RNAi Screen Reveals Deubiquitinases as New Regulators of Notch Signaling. [FBrf0220374] Booker et al., 2011, BMC Genomics 12: 50 False negative rates in Drosophila cell-based RNAi screens: a case study. [FBrf0212969] Du et al., 2011, PLoS ONE 6(9): e24168 In Vivo RNAi Screen Reveals Neddylation Genes as Novel Regulators of Hedgehog Signaling. [FBrf0215574] Neumüller et al., 2011, Cell Stem Cell 8(5): 580--593 Genome-Wide Analysis of Self-Renewal in Drosophila Neural Stem Cells by Transgenic RNAi. [FBrf0213621] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...