FB2025_01 , released February 20, 2025
Gene: Dmel\Acp26Ab
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General Information
Symbol
Dmel\Acp26Ab
Species
D. melanogaster
Name
Accessory gland protein 26Ab
Annotation Symbol
CG9024
Feature Type
protein_coding_gene
FlyBase ID
FBgn0002856
Gene Model Status
Current
Stock Availability
3 publicly available
Gene Summary
Accessory gland protein 26Ab (Acp26Ab) encodes a peptide that is produced by male accessory glands and transferred to females during mating. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)
All Summaries
Also Known As

Mst26Ab, Acp26A, mst355b, Acp26Aab, mst 355b

Key Links
Genomic Location
Cytogenetic map
26A1-26A1
Sequence location
Recombination map
2-18
RefSeq locus
NT_033779 REGION:5892298..5892862
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (4 terms)
Molecular Function (0 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (0 terms)
Biological Process (2 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
involved_in sexual reproduction
inferred from expression pattern
(Swanson et al., 2001)
inferred from high throughput expression pattern
(Findlay et al., 2009, Findlay et al., 2008, Mueller et al., 2005)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
involved_in mating behavior
inferred from electronic annotation with InterPro:IPR008392
(InterPro Project Members, 2004-)
Cellular Component (2 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
located_in extracellular space
inferred from high throughput direct assay
(Findlay et al., 2009, Findlay et al., 2008)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
located_in extracellular region
inferred from electronic annotation with InterPro:IPR008392
(InterPro Project Members, 2004-)
inferred from sequence model
(Swanson et al., 2001)
located_in extracellular space
inferred from sequence model
(Mueller et al., 2005)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Summaries
Gene Snapshot
Accessory gland protein 26Ab (Acp26Ab) encodes a peptide that is produced by male accessory glands and transferred to females during mating. [Date last reviewed: 2019-03-07]
Protein Function (UniProtKB)
This protein is transferred from male to female during mating and may affect egglaying and behavior after mating.
(UniProt, P10334)
Phenotypic Description (Red Book; Lindsley and Zimm 1992)
Mst26Ab
Expressed in males, but not in females; also expressed in the germ-cell-free sons of tud females as well as in XX individuals that are homozygous for dsx, ix, tra2, or tra. Presence of tra2+ activity in XX flies during a part of the third larval instar is sufficient to repress expression of Mst26Ab and prevent accessory-gland formation and the production of Mst26Ab transcript.
Gene Model and Products
Number of Transcripts
3
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\Acp26Ab for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry P10334)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Gene model reviewed during 5.51

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Acp26Ab-RA
FBtr0079156
NM_057297
453
90
Acp26Ab-RB
FBtr0079157
NM_134272
504
90
Acp26Ab-RC
FBtr0342618
NM_001298717
408
90
Additional Transcript Data and Comments
Reported size (kB)

0.5 (northern blot)

(Monsma and Wolfner, 1988)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
Acp26Ab-PA
FBpp0078787
10.2
90
4.52
P10334
NP_476645
AAF52297
Acp26Ab-PB
FBpp0078788
10.2
90
4.52
P10334
NP_599099
AAN10560
Acp26Ab-PC
FBpp0309554
10.2
90
4.52
P10334
NP_001285646
AHN54161
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

90 aa isoforms: Acp26Ab-PA, Acp26Ab-PB, Acp26Ab-PC
Additional Polypeptide Data and Comments
Reported size (kDa)

90 (aa); 11-14 (kD observed); 10 (kD predicted)

(Monsma and Wolfner, 1988)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Nucleotide / Polypeptide Records
 
UniProt
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Acp26Ab using the Feature Mapper tool.

External Data
Crossreferences
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.04

Transcript Expression
northern blot
Stage
Tissue/Position (including subcellular localization)
Reference
pupal stage & adult stage
(Chapman and Wolfner, 1988)
adult stage | male
male accessory gland
(Monsma and Wolfner, 1988)
(Monsma et al., 1990)
Additional Descriptive Data

In unmated adult males, Acp26Ab transcript levels remain constant for the first 14 days of adult life and were not tested beyond that.

(Monsma et al., 1990)

Acp26Ab transcripts were assayed in hand-sorted staged pupae. They are first detected at pupal stage 13. They are present at adult levels by one day after eclosion and persist at these levels for at least 10 days. Determination of expression occurs during the third larval instar which correlates with the time of determination of the accessory glands.

(Chapman and Wolfner, 1988)

Acp26Aa transcripts are detected in RNA from adult males and specifically in accessory gland RNA.

(Monsma and Wolfner, 1988)
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage | female
adult hemolymph
(Monsma et al., 1990)
genital chamber
(Monsma et al., 1990)
adult stage | male
male accessory gland
(Monsma et al., 1990)
male accessory gland main cell
(Monsma et al., 1990)
male accessory gland secondary cell
(Monsma et al., 1990)
western blot
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
female reproductive system

Comment: mated females

(Ravi Ram et al., 2005)
adult hemolymph

Comment: mated females

(Ravi Ram et al., 2005)
uterus

Comment: mated females

(Ravi Ram et al., 2005)
Additional Descriptive Data

Acp26Ab is transferred to the female at the time of mating and persists for about 2-3 hours in the female reproductive tract. Acp26Ab enters the female hemolymph. It is detected in the uterus.

(Ravi Ram et al., 2005)

Acp26Ab protein is not detectable in stage P13 pupae but is detectable in accessory glands dissected from adult males 3-4 hours after eclosion. Levels reach a plateau about 3-5 days after eclosion. Levels remain high in accessory gland secretions, even in 30 day old virgin males. Staining is present in both the main cells and the secondary cells of the accessory gland. In 5 day and older virgin males, protein is absent in the main cells but is present in the secondary cells where it is concentrated in large vesicles. Abundance of Acp2 Ab protein is stimulated by copulation. Acp26Ab protein is detected in the female genital tract 10 minutes after the start of copulation as well as in the hemolymph. It is no longer detected in the hemolymph by 2 hours after copulation and in the female genital tract by 2-3 hours after copulation. Protein was not detected in any other tissue examined.

(Monsma et al., 1990)
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in extracellular space
inferred from high throughput direct assay
(Findlay et al., 2009, Findlay et al., 2008)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Acp26Ab in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 10 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 4 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Acp26Ab
Transgenic constructs containing regulatory region of Acp26Ab
Aberrations (Deficiencies and Duplications) ( 2 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4Mef2.PR
viable, with Scer\GAL4pnr-MD237
Phenotype manifest in
Allele
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (0)
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (0)
Mus musculus (laboratory mouse) (0)
Xenopus tropicalis (Western clawed frog) (0)
Danio rerio (Zebrafish) (0)
Caenorhabditis elegans (Nematode, roundworm) (0)
Anopheles gambiae (African malaria mosquito) (0)
Arabidopsis thaliana (thale-cress) (0)
Saccharomyces cerevisiae (Brewer's yeast) (0)
Schizosaccharomyces pombe (Fission yeast) (0)
Escherichia coli (enterobacterium) (0)
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:Acp26Ab. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    esyN Network Diagram
    Other Interaction Browsers
    Summary of Genetic Interactions
    esyN Network Diagram
    Other Interaction Browsers
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    External Data
    Linkouts
    DroID - A comprehensive database of gene and protein interactions.
    Pathways
    Signaling Pathways (FlyBase)
    Metabolic Pathways
    FlyBase
    External Links
    External Data
    Linkouts
    Class of Gene
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    2L
    Recombination map
    2-18
    Cytogenetic map
    26A1-26A1
    Sequence location
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    26A1-26A1
    Limits computationally determined from genome sequence between P{lacW}l(2)k06502k06502 and P{EP}bchsEP2299&P{EP}GpdhEP466
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    25F-26A
    (determined by in situ hybridisation)
    (Monsma and Wolfner, 1988)
    Experimentally Determined Recombination Data
    Location

    2-18

    (FlyBase, 2016)

    2-19.0

    (Comeron et al., 2012)
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (3)
    Genomic Clones (18)
     

    Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

    cDNA Clones (3)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequenced
    BDGP DGC clones
    Other clones
      Drosophila Genomics Resource Center cDNA clones

      For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

      cDNA Clones, End Sequenced (ESTs)
      BDGP DGC clones
        Other clones
        RNAi and Array Information
        Linkouts
        DRSC - Results frm RNAi screens
        Antibody Information
        Laboratory Generated Antibodies
         

        polyclonal

        (Monsma and Wolfner, 1988)
        Commercially Available Antibodies
         
        Cell Line Information
        Publicly Available Cell Lines
         
          Other Stable Cell Lines
           
            Other Comments

            If females remate frequently relative to the duration of sperm storage and rate of sperm use, sperm displacement may be an important component of male reproductive success. Significant association has been found between Acp26Ab mutant male sperm and the ability to resist displacement by subsequent sperm. There is no correlation between the ability to displace resident sperm and the ability to resist being displaced by subsequent sperm, this lack of correlation suggests that different mechanisms mediate that the two components of sperm displacement.

            (Clark et al., 1995)

            Acp26Aa and Acp26Ab are encoded by separate mRNAs. Regulatory elements for Acp26Ab lie within a fragment containing the intergenic region and transcribed sequences of Acp26Aa and Acp26Ab.

            (Park et al., 1994)

            Sequence and restriction map polymorphism studied in 10 isogenic lines of D.melanogaster, and one line each of D.sechellia, D.mauritiana and D.simulans : pattern suggests selection in or near Acp26Aa-Acp26Ab region has played a part in the history of these genes.

            (Aguade et al., 1992)

            Expressed in males, not females. Presence of tra2+ activity in XX flies during a part of the third larval instar is sufficient to repress expression of Acp26Ab and prevent accessory-gland formation and the production of Acp26Ab transcript.

            (Lindsley and Zimm, 1992)

            The developmental and mating-stimulated expression of Acp26Ab, and the transfer of the protein product to and fates in the mated female has been studied.

            (Monsma et al., 1990)
            Relationship to Other Genes
            Source for database merge of
            Additional comments
            Nomenclature History
            Source for database identify of
            Nomenclature comments
            Etymology
            Synonyms and Secondary IDs (15)
            Datasets (0)
            Study focus (0)
            Experimental Role
            Project
            Project Type
            Title
            Study result (0)
            Result
            Result Type
            Title
            External Crossreferences and Linkouts ( 233 )
            Sequence Crossreferences
            NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
            GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
            GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
            RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
            UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
            UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
            UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
            Other crossreferences
            AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
            BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
            DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
            EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
            FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
            FlyMine - An integrated database for Drosophila genomics
            InterPro - A database of protein families, domains and functional sites
            KEGG Genes - Molecular building blocks of life in the genomic space.
            MARRVEL_MODEL - MARRVEL (model organism gene)
            Linkouts
            Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
            DroID - A comprehensive database of gene and protein interactions.
            DRSC - Results frm RNAi screens
            FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
            FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
            Flygut - An atlas of the Drosophila adult midgut
            References (110)