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General Information
Symbol
Dmel\Acp26Ab
Species
D. melanogaster
Name
Accessory gland protein 26Ab
Annotation Symbol
CG9024
Feature Type
FlyBase ID
FBgn0002856
Gene Model Status
Stock Availability
Gene Snapshot
Accessory gland protein 26Ab (Acp26Ab) encodes a peptide that is produced by male accessory glands and transferred to females during mating. [Date last reviewed: 2019-03-07]
Also Known As

Mst26Ab, Acp26A, Acp26Aab, mst355b, mst 355b

Key Links
Genomic Location
Cytogenetic map
Sequence location
2L:5,892,298..5,892,862 [-]
Recombination map

2-18

RefSeq locus
NT_033779 REGION:5892298..5892862
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Protein Family (UniProt)
-
Molecular Function (GO)
[Detailed GO annotations]
Experimental Evidence
-
Predictions / Assertions
-
Summaries
Protein Function (UniProtKB)
This protein is transferred from male to female during mating and may affect egglaying and behavior after mating.
(UniProt, P10334)
Phenotypic Description (Red Book; Lindsley and Zimm 1992)
Mst26Ab
Expressed in males, but not in females; also expressed in the germ-cell-free sons of tud females as well as in XX individuals that are homozygous for dsx, ix, tra2, or tra. Presence of tra2+ activity in XX flies during a part of the third larval instar is sufficient to repress expression of Mst26Ab and prevent accessory-gland formation and the production of Mst26Ab transcript.
Gene Model and Products
Number of Transcripts
3
Number of Unique Polypeptides
1

Please see the GBrowse view of Dmel\Acp26Ab or the JBrowse view of Dmel\Acp26Ab for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Gene model reviewed during 5.51

Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0079156
453
90
FBtr0079157
504
90
FBtr0342618
408
90
Additional Transcript Data and Comments
Reported size (kB)

0.5 (northern blot)

Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0078787
10.2
90
4.52
FBpp0078788
10.2
90
4.52
FBpp0309554
10.2
90
4.52
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

90 aa isoforms: Acp26Ab-PA, Acp26Ab-PB, Acp26Ab-PC
Additional Polypeptide Data and Comments
Reported size (kDa)

90 (aa); 11-14 (kD observed); 10 (kD predicted)

Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Nucleotide / Polypeptide Records
 
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Acp26Ab using the Feature Mapper tool.

External Data
Crossreferences
Linkouts
Gene Ontology (4 terms)
Molecular Function (0 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (0 terms)
Biological Process (2 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
inferred from high throughput expression pattern
inferred from expression pattern
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
inferred from electronic annotation with InterPro:IPR008392
(assigned by InterPro )
Cellular Component (2 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
inferred from high throughput direct assay
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
inferred from electronic annotation with InterPro:IPR008392
(assigned by InterPro )
inferred from sequence model
inferred from sequence model
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
northern blot
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data

In unmated adult males, Acp26Ab transcript levels remain constant for the first 14 days of adult life and were not tested beyond that.

Acp26Ab transcripts were assayed in hand-sorted staged pupae. They are first detected at pupal stage 13. They are present at adult levels by one day after eclosion and persist at these levels for at least 10 days. Determination of expression occurs during the third larval instar which correlates with the time of determination of the accessory glands.

Acp26Aa transcripts are detected in RNA from adult males and specifically in accessory gland RNA.

Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
western blot
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data

Acp26Ab is transferred to the female at the time of mating and persists for about 2-3 hours in the female reproductive tract. Acp26Ab enters the female hemolymph. It is detected in the uterus.

Acp26Ab protein is not detectable in stage P13 pupae but is detectable in accessory glands dissected from adult males 3-4 hours after eclosion. Levels reach a plateau about 3-5 days after eclosion. Levels remain high in accessory gland secretions, even in 30 day old virgin males. Staining is present in both the main cells and the secondary cells of the accessory gland. In 5 day and older virgin males, protein is absent in the main cells but is present in the secondary cells where it is concentrated in large vesicles. Abundance of Acp2 Ab protein is stimulated by copulation. Acp26Ab protein is detected in the female genital tract 10 minutes after the start of copulation as well as in the hemolymph. It is no longer detected in the hemolymph by 2 hours after copulation and in the female genital tract by 2-3 hours after copulation. Protein was not detected in any other tissue examined.

Marker for
 
Subcellular Localization
CV Term
Evidence
References
inferred from high throughput direct assay
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Acp26Ab in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Flygut - An atlas of the Drosophila adult midgut
Images
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 10 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 4 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Acp26Ab
Transgenic constructs containing regulatory region of Acp26Ab
Deletions and Duplications ( 2 )
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Phenotype manifest in
Allele
Orthologs
Human Orthologs (via DIOPT v7.1)
Homo sapiens (Human) (0)
No records found.
Model Organism Orthologs (via DIOPT v7.1)
Mus musculus (laboratory mouse) (0)
No records found.
Rattus norvegicus (Norway rat) (0)
No records found.
Xenopus tropicalis (Western clawed frog) (0)
No records found.
Danio rerio (Zebrafish) (0)
No records found.
Caenorhabditis elegans (Nematode, roundworm) (0)
No records found.
Arabidopsis thaliana (thale-cress) (0)
No records found.
Saccharomyces cerevisiae (Brewer's yeast) (0)
No records found.
Schizosaccharomyces pombe (Fission yeast) (0)
No records found.
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG09190KAK )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila sechellia
Drosophila erecta
Drosophila yakuba
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila persimilis
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( None identified )
No non-Drosophilid orthologies identified
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( None identified )
No non-Dipteran orthologies identified
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( None identified )
No non-Insect Arthropod orthologies identified
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( None identified )
No non-Arthropod Metazoa orthologies identified
Paralogs
Paralogs (via DIOPT v7.1)
Drosophila melanogaster (Fruit fly) (0)
No records found.
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    esyN Network Diagram
    Interactions Browser
    Summary of Genetic Interactions
    esyN Network Diagram
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    External Data
    Linkouts
    DroID - A comprehensive database of gene and protein interactions.
    Pathways
    Signaling Pathways (FlyBase)
    Metabolic Pathways
    External Data
    Linkouts
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    2L
    Recombination map

    2-18

    Cytogenetic map
    Sequence location
    2L:5,892,298..5,892,862 [-]
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    26A1-26A1
    Limits computationally determined from genome sequence between P{lacW}l(2)k06502k06502 and P{EP}bchsEP2299&P{EP}GpdhEP466
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    25F-26A
    (determined by in situ hybridisation)
    Experimentally Determined Recombination Data
    Location
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (3)
    Genomic Clones (18)
     

    Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

    cDNA Clones (3)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequences
    BDGP DGC clones
    Other clones
      Drosophila Genomics Resource Center cDNA clones

      For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

      cDNA Clones, End Sequenced (ESTs)
      BDGP DGC clones
        Other clones
        RNAi and Array Information
        Linkouts
        DRSC - Results frm RNAi screens
        GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
        Antibody Information
        Laboratory Generated Antibodies
        Commercially Available Antibodies
         
        Other Information
        Relationship to Other Genes
        Source for database identify of
        Source for database merge of
        Additional comments
        Other Comments

        If females remate frequently relative to the duration of sperm storage and rate of sperm use, sperm displacement may be an important component of male reproductive success. Significant association has been found between Acp26Ab mutant male sperm and the ability to resist displacement by subsequent sperm. There is no correlation between the ability to displace resident sperm and the ability to resist being displaced by subsequent sperm, this lack of correlation suggests that different mechanisms mediate that the two components of sperm displacement.

        Acp26Aa and Acp26Ab are encoded by separate mRNAs. Regulatory elements for Acp26Ab lie within a fragment containing the intergenic region and transcribed sequences of Acp26Aa and Acp26Ab.

        Sequence and restriction map polymorphism studied in 10 isogenic lines of D.melanogaster, and one line each of D.sechellia, D.mauritiana and D.simulans : pattern suggests selection in or near Acp26Aa-Acp26Ab region has played a part in the history of these genes.

        Expressed in males, not females. Presence of tra2+ activity in XX flies during a part of the third larval instar is sufficient to repress expression of Acp26Ab and prevent accessory-gland formation and the production of Acp26Ab transcript.

        The developmental and mating-stimulated expression of Acp26Ab, and the transfer of the protein product to and fates in the mated female has been studied.

        Origin and Etymology
        Discoverer
        Etymology
        Identification
        External Crossreferences and Linkouts ( 230 )
        Sequence Crossreferences
        NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
        RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
        UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
        UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
        Other crossreferences
        BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
        Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
        Flygut - An atlas of the Drosophila adult midgut
        GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
        InterPro - A database of protein families, domains and functional sites
        KEGG Genes - Molecular building blocks of life in the genomic space.
        modMine - A data warehouse for the modENCODE project
        Linkouts
        DroID - A comprehensive database of gene and protein interactions.
        DRSC - Results frm RNAi screens
        FLIGHT - Cell culture data for RNAi and other high-throughput technologies
        FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
        FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
        FlyMine - An integrated database for Drosophila genomics
        Synonyms and Secondary IDs (15)
        Datasets (0)
        Study focus (0)
        Experimental Role
        Project
        Project Type
        Title
        References (109)