General Information
Symbol
Dmel\MtnA
Species
D. melanogaster
Name
Metallothionein A
Annotation Symbol
CG9470
Feature Type
FlyBase ID
FBgn0002868
Gene Model Status
Stock Availability
Gene Snapshot
Metallothionein A is a member of the Metallothionein family whose transcript level is higher than that of the other four Drosophila metallothioneins. Transcription is strongly induced by copper and cadmium ions and this induction depends on the transcription factor MTF-1. [Date last reviewed: 2016-08-25]
Also Known As
Mtn
Genomic Location
Cytogenetic map
Sequence location
3R:9,783,407..9,784,370 [-]
Recombination map
3-49
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
GO Summary Ribbons
Families, Domains and Molecular Function
Gene Group Membership (FlyBase)
Protein Family (UniProt, Sequence Similarities)
Belongs to the metallothionein superfamily. Type 5 family. (P04357)
Molecular Function (see GO section for details)
Experimental Evidence
Predictions / Assertions
-
Summaries
Gene Group Membership
METALLOTHIONEINS -
Metallothioneins constitute a family of cysteine-rich, low molecular weight metal-binding proteins. They bind physiological metals, such as zinc and copper, as well as nonessential, toxic heavy metals, such as cadmium, mercury, and silver. They play an essential role in heavy metal detoxification and in maintaining the homeostasis of essential trace metals. They are also involved in the protection against free radicals and oxidative stress. (Adapted from FBrf0215591).
UniProt Contributed Function Data
This protein binds cations of several transition elements. It is thought to be involved in detoxification processes.
(UniProt, P04357)
Phenotypic Description from the Red Book (Lindsley and Zimm 1992)
Mtn: Metallothionein (G. Maroni)
Codes for metallothionein, a metal binding protein. Inducible by ingestion of Cu or Cd (but not Zn) ions. In larvae, it is expressed mainly in the midgut. No point mutations are available, but Dp(3;3)Mtn+H22 shows increased tolerance to Cd.
Gene Model and Products
Number of Transcripts
2
Number of Unique Polypeptides
1

Please see the GBrowse view of Dmel\MtnA or the JBrowse view of Dmel\MtnA for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.47
Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0082158
329
40
FBtr0334686
700
40
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0081636
3.9
40
7.85
FBpp0306744
3.9
40
7.85
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

40 aa isoforms: MtnA-PA, MtnA-PB
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Domain
All cysteine residues are arranged in C-X-C groups. These are thought to be the metal-binding sites in other metallothioneins.
(UniProt, P04357)
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Nucleotide / Polypeptide Records
 
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\MtnA using the Feature Mapper tool.

External Data
Crossreferences
Linkouts
Gene Ontology (3 terms)
Molecular Function (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
inferred from direct assay
Terms Based on Predictions or Assertions (0 terms)
Biological Process (2 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
inferred from mutant phenotype
inferred from direct assay
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
inferred from sequence or structural similarity with FLYBASE:MtnE; FB:FBgn0262146
Cellular Component (0 terms)
There is no information supporting a Cellular Component term for this gene. (Based on a review of available data on: 2013-03-07 )
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (0 terms)
Expression Data
Transcript Expression
expression microarray
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\MtnA in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Flygut - An atlas of the Drosophila adult midgut
Images
Alleles, Insertions, Transgenic Constructs and Phenotypes
Classical and Insertion Alleles ( 6 )
For All Classical and Insertion Alleles Show
 
Allele of MtnA
Class
Mutagen
Associated Insertion
Stocks
Known lesion
    0
    Yes
    Other relevant insertions
    Transgenic Constructs ( 15 )
    Deletions and Duplications ( 3 )
    Summary of Phenotypes
    Orthologs
    Human Orthologs (via DIOPT v7.1)
    Homo sapiens (Human) (0)
    No orthologs reported.
    Model Organism Orthologs (via DIOPT v7.1)
    Mus musculus (laboratory mouse) (0)
    No orthologs reported.
    Rattus norvegicus (Norway rat) (0)
    No orthologs reported.
    Xenopus tropicalis (Western clawed frog) (0)
    No orthologs reported.
    Danio rerio (Zebrafish) (0)
    No orthologs reported.
    Caenorhabditis elegans (Nematode, roundworm) (2)
    1 of 15
    Yes
    Yes
    1 of 15
    Yes
    Yes
    Arabidopsis thaliana (thale-cress) (1)
    1 of 9
    Yes
    Yes
    Saccharomyces cerevisiae (Brewer's yeast) (2)
    1 of 15
    No
    Yes
    1 of 15
    No
    No
    Schizosaccharomyces pombe (Fission yeast) (0)
    No orthologs reported.
    Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG09190KXM )
    Organism
    Common Name
    Gene
    AAA Syntenic Ortholog
    Multiple Dmel Genes in this Orthologous Group
    Drosophila melanogaster
    fruit fly
    Drosophila simulans
    Drosophila sechellia
    Drosophila erecta
    Drosophila yakuba
    Drosophila ananassae
    Drosophila pseudoobscura pseudoobscura
    Drosophila persimilis
    Drosophila willistoni
    Drosophila virilis
    Drosophila mojavensis
    Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150K44 )
    Organism
    Common Name
    Gene
    Multiple Dmel Genes in this Orthologous Group
    Lucilia cuprina
    Australian sheep blowfly
    Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( None identified )
    No non-Dipteran orthologies identified
    Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( None identified )
    No non-Insect Arthropod orthologies identified
    Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( None identified )
    No non-Arthropod Metazoa orthologies identified
    Human Disease Model Data
    FlyBase Human Disease Model Reports
      Alleles Reported to Model Human Disease (Disease Ontology)
      Download
      Models ( 0 )
      Allele
      Disease
      Evidence
      References
      Interactions ( 2 )
      Allele
      Disease
      Interaction
      References
      Comments ( 0 )
       
      Human Orthologs (via DIOPT v7.1)
      Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
      Homo sapiens (Human)
      Gene name
      Score
      OMIM
      OMIM Phenotype
      Complementation?
      Transgene?
      Functional Complementation Data
      Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
      Interactions
      Summary of Physical Interactions
      esyN Network Diagram
      Show neighbor-neighbor interactions:
      Select Layout:
      Legend:
      Protein
      RNA
      Selected Interactor(s)
      Interactions Browser

      Please look at the Interaction Group reports for full details of the physical interactions
      protein-protein
      Interacting group
      Assay
      References
      Summary of Genetic Interactions
      esyN Network Diagram
      esyN Network Key:
      Suppression
      Enhancement

      Please look at the allele data for full details of the genetic interactions
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      External Data
      Linkouts
      BioGRID - A database of protein and genetic interactions.
      DroID - A comprehensive database of gene and protein interactions.
      InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
      Pathways
      Gene Group - Pathway Membership (FlyBase)
      External Data
      Linkouts
      Genomic Location and Detailed Mapping Data
      Chromosome (arm)
      3R
      Recombination map
      3-49
      Cytogenetic map
      Sequence location
      3R:9,783,407..9,784,370 [-]
      FlyBase Computed Cytological Location
      Cytogenetic map
      Evidence for location
      85E9-85E9
      Limits computationally determined from genome sequence between P{EP}CrcEP3006 and P{lacW}s2681
      Experimentally Determined Cytological Location
      Cytogenetic map
      Notes
      References
      85E10-85E15
      (determined by in situ hybridisation)
      85E-85E
      (determined by in situ hybridisation)
      Experimentally Determined Recombination Data
      Location
      Left of (cM)
      Right of (cM)
      Notes
      Stocks and Reagents
      Stocks (6)
      Genomic Clones (8)
       

      Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

      cDNA Clones (313)
       

      Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

      cDNA clones, fully sequences
      BDGP DGC clones
      Other clones
      Drosophila Genomics Resource Center cDNA clones

      For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

      cDNA Clones, End Sequenced (ESTs)
      Other clones
      RNAi and Array Information
      Linkouts
      DRSC - Results frm RNAi screens
      GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
      Antibody Information
      Laboratory Generated Antibodies
       
      Commercially Available Antibodies
       
      Other Information
      Relationship to Other Genes
      Source for database identify of
      Source for database merge of
      Source for merge of: MtnA BcDNA:GH18460
      Source for merge of: MtnA anon-WO0153538.42 anon-WO0153538.44 anon-WO0153538.49
      Additional comments
      Source for merge of MtnA BcDNA:GH18460 was a shared cDNA ( date:030728 ).
      Source for merge of MtnA anon-WO0153538.42 anon-WO0153538.44 anon-WO0153538.49 was sequence comparison ( date:051113 ).
      Other Comments
      In a sample of 79 genes with multiple introns, 33 showed significant heterogeneity in G+C content among introns of the same gene and significant positive correspondence between the intron and the third codon position G+C content within genes. These results are consistent with selection adding against preferred codons at the start of genes.
      MtnA promoter expression induced by CuSO4 in recombinant baculovirus is inducible but 'leaky'.
      Codes for metallothionein, a metal binding protein. Inducible by ingestion of Cu or Cd (but not Zn) ions. In larvae, it is expressed mainly in the midgut. No point mutations are available, but Dp(3;3)Mtn+H22 shows increased tolerance to Cd.
      MtnA and MtnB gene structure and expression pattern have been compared.
      Metal-regulated and tissue-specific expression of MtnA requires no more than 373bp upstream of the transcription start site. MtnA sequences from -130 to -6bp are sufficient to confer metal-regulated expression to MtnA-Hsim\TK promoter fusion constructs.
      Origin and Etymology
      Discoverer
      Etymology
      Identification
      External Crossreferences and Linkouts ( 41 )
      Crossreferences
      NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
      GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
      GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
      RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
      UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
      UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
      Other crossreferences
      InterPro - A database of protein families, domains and functional sites
      Linkouts
      BioGRID - A database of protein and genetic interactions.
      Drosophila Genomics Resource Center - Drosophila Genomics Resource Center cDNA clones
      DroID - A comprehensive database of gene and protein interactions.
      DRSC - Results frm RNAi screens
      FLIGHT - Cell culture data for RNAi and other high-throughput technologies
      FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
      Flygut - An atlas of the Drosophila adult midgut
      FlyMine - An integrated database for Drosophila genomics
      GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
      InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
      KEGG Genes - Molecular building blocks of life in the genomic space.
      modMine - A data warehouse for the modENCODE project
      Synonyms and Secondary IDs (14)
      Reported As
      Symbol Synonym
      BcDNA:GH18460
      anon-WO0153538.42
      anon-WO0153538.44
      anon-WO0153538.49
      Secondary FlyBase IDs
      • FBgn0045904
      • FBgn0045909
      • FBgn0045911
      • FBgn0064964
      Datasets (0)
      Study focus (0)
      Experimental Role
      Project
      Project Type
      Title
      References (137)