FlyBase Gene Report: Dmel\mud

Neuropile of mushroom bodies (usually in dorsal brain) is missing (Heisenberg, 1980); penetrance for this anatomical phenotype (on which criterion the mutant was isolated) ca. 90%. Calyces that should innervate pedunculi and lobes of mushroom bodies are enlarged, as are antennal lobes in anterior brain. At sites of calyces, large numbers of thin axons form distinct lobes outside main neuropile of brain. Viability of mutant females poor, and they are sterile as well. Development of mushroom bodies apparently normal until pupation, when "reconstruction" of this bilaterally paired brain entity (this process being a normal feature of wild-type metamorphosis) is aberrant (Technau and Heisenberg, 1982). Supernumerary neuroblasts observed in the larval brain, owing to anomalous proliferation of such cells postembryonically.

Summary (Interactive Fly)

involved in spindle orientation during asymmetric cell division - asymmetrically localized Partner of inscuteable-Gαi complexes regulate spindle orientation by directly binding to Mud which in turn binds microtubules

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the GBrowse view of Dmel\mud or the JBrowse view of Dmel\mud for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.39

Apparent introns within longest coding exon in cDNAs and RNA-seq junctions within the same region are likely to be artifacts; possibly caused by reverse transcriptase template-switching.

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.45

Gene model reviewed during 5.55

Sequence Ontology: Class of Gene

gene_with_edited_transcript

(Graveley et al., 2011)

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

mud-RF

FBtr0308325

NM_167400

7938

2394

mud-RG

FBtr0308326

NM_080495

6286

1933

mud-RH

FBtr0308327

NM_167399

8457

2567

mud-RI

FBtr0308328

NM_001258736

8643

2113

mud-RJ

FBtr0308329

NM_001258737

7097

2165

mud-RK

FBtr0332621

NM_001272615

6223

1912

mud-RL

FBtr0332622

NM_001272616

8259

2501

Additional Transcript Data and Comments

Reported size (kB)

6.003 (longest cDNA)

(Guan et al., 2000)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

mud-PF

FBpp0300644

274.5

2394

5.47

NP_727770

AAF48362

mud-PG

FBpp0300645

223.3

1933

5.02

NP_536743

AAN09584

mud-PH

FBpp0300646

294.1

2567

5.46

NP_727769

AAN09583

mud-PI

FBpp0300647

242.8

2113

5.06

NP_001245665

AFH07379

mud-PJ

FBpp0300648

248.9

2165

5.14

NP_001245666

AFH07380

mud-PK

FBpp0304867

220.8

1912

5.02

NP_001259544

AGB95386

mud-PL

FBpp0304868

286.5

2501

5.50

NP_001259545

AGB95387

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

2501, 1871 (aa)

(Guan et al., 2000)

Comments

Each mud protein isoform is predicted to have a long coiled-coil in the central region, and a carboxy-terminal transmembrane domain. The region predicted to form a coiled-coil includes a segment of 42 amino acids that is repeated 10 times. The 1871 amino acid isoform is predicted to have a single transmembrane segment, whereas the two longer isoforms are predicted to have two transmembrane segments each.

(Guan et al., 2000)

External Data

Crossreferences

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\mud using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

mud_1

Linkouts

Gene Ontology (17 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

DNA binding

inferred from direct assay

(Smith and Prehoda, 2011)

protein binding

inferred from physical interaction with FLYBASE:pins; FB:FBgn0040080

(Nipper et al., 2007)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (7 terms)

Terms Based on Experimental Evidence (7 terms)

CV Term

Evidence

References

asymmetric neuroblast division

inferred from mutant phenotype

(Cabernard and Doe, 2009)

axon guidance

inferred from genetic interaction with FLYBASE:fra; FB:FBgn0011592

(Cate et al., 2016)

inferred from genetic interaction with FLYBASE:NetA; FB:FBgn0015773, FLYBASE:NetB; FB:FBgn0015774

(Cate et al., 2016)

establishment of mitotic spindle orientation

inferred from mutant phenotype

(Bosveld et al., 2016, Wang et al., 2011, Ségalen et al., 2010)

inferred from genetic interaction with FLYBASE:dsh; FB:FBgn0000499

(Ségalen et al., 2010)

microtubule anchoring at centrosome

inferred from mutant phenotype

(Wang et al., 2011)

mushroom body development

inferred from mutant phenotype

(de Belle and Heisenberg, 1996)

spindle assembly involved in female meiosis II

inferred from mutant phenotype

(Yu et al., 2006)

thermosensory behavior

inferred from mutant phenotype

(Hong et al., 2008)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

mushroom body development

traceable author statement

(Guan et al., 2000)

Cellular Component (8 terms)

Terms Based on Experimental Evidence (7 terms)

CV Term

Evidence

References

apical cortex

inferred from direct assay

(Nipper et al., 2007)

basal cortex

inferred from direct assay

(Nipper et al., 2007)

cell cortex

inferred from direct assay

(Wee et al., 2011)

centrosome

inferred from direct assay

(Nipper et al., 2007)

spindle

inferred from direct assay

(Wang et al., 2011)

spindle midzone

inferred from direct assay

(Yu et al., 2006)

tricellular tight junction

inferred from direct assay

(Bosveld et al., 2016)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

nucleoplasm

inferred from biological aspect of ancestor with PANTHER:PTN000431519

(assigned by GO_Central )

(Gaudet et al., 2011)

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

(Guan et al., 2000)

larval stage

(Guan et al., 2000)

larval stage | male

(Guan et al., 2000)

adult stage

(Guan et al., 2000)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

day 5 of adulthood & male -- day 7 of adulthood & male

spermatozoon

(Wasbrough et al., 2010)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

apical cortex

inferred from direct assay

(Nipper et al., 2007)

basal cortex

inferred from direct assay

(Nipper et al., 2007)

cell cortex

inferred from direct assay

(Wee et al., 2011)

centrosome

inferred from direct assay

(Nipper et al., 2007)

spindle

inferred from direct assay

(Wang et al., 2011)

spindle midzone

inferred from direct assay

(Yu et al., 2006)

tricellular tight junction

inferred from direct assay

(Bosveld et al., 2016)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\mud in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\mud

View exonic expression by tissue for Dmel\mud

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0002873

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG12047-RC;CG12047-RC;CG12047-RA

Flygut - An atlas of the Drosophila adult midgut

FBgn0002873

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, and Transgenic Constructs

Classical and Insertion Alleles ( 11 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 21 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of mud

Transgenic constructs containing regulatory region of mud

Deletions and Duplications ( 11 )

Disrupted in

Df(1)ben^CO1

(Guan et al., 2000)

Df(1)ben^CO2

(Guan et al., 2000)

Df(1)BSC628

(Christensen et al., 2008.9.29)

Df(1)BSC705

(Christensen et al., 2008.12.28)

Df(1)BSC857

(Cook et al., 2009.11.25)

Df(1)KA9

(Guan et al., 2000)

Df(1)RK3

(Guan et al., 2000)

Not disrupted in

Df(1)g

(Guan et al., 2000)

Df(1)NP5

(Guan et al., 2000, Mitchell, 1997.2.17)

Df(1)RK5

(Guan et al., 2000)

T(1;Y)B146

(Scott, 2008.5.20)

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal | female

lethal - all die before end of embryonic stage | non-rescuable maternal effect | recessive

mud⁴

partially lethal - majority die

partially lethal - majority die | female limited

Sterility

Allele

female sterile

male fertile

sterile (with Df(1)KA9)

mud¹

Other Phenotypes

Allele

chemical sensitive

mud⁴

chemical sensitive (with mud¹)

mud⁴

chemical sensitive (with mud⁴)

mud¹

chemical sensitive | codominant

mud⁴

hyperplasia | third instar larval stage

mud²

increased cell number | larval stage

mud²

increased occurrence of cell division | embryonic stage | heat sensitive

mud²

locomotor behavior defective

mitotic cell cycle defective | P-stage

neuroanatomy defective | embryonic stage

mud³

neuroanatomy defective | embryonic stage, with Scer\GAL4^eg.PU

mud^EY20197

some die during embryonic stage | recessive

mud⁴

wild-type

Phenotype manifest in

Allele

abdominal 2 dorso-lateral neuroblast

mud²

abdominal 2 ventro-medial neuroblast

mud²

abdominal 3 dorso-lateral neuroblast

mud²

abdominal 3 ventro-medial neuroblast

mud²

abdominal 4 dorso-lateral neuroblast

mud²

abdominal 4 ventro-medial neuroblast

mud²

abdominal 5 dorso-lateral neuroblast

mud²

abdominal 5 ventro-medial neuroblast

mud²

abdominal 6 dorso-lateral neuroblast

mud²

abdominal 6 ventro-medial neuroblast

mud²

abdominal 7 dorso-lateral neuroblast

mud²

abdominal 7 ventro-medial neuroblast

mud²

adult central complex

adult mushroom body

antennal lobe

mud¹

astral microtubule

mud¹

calyx of adult mushroom body

centrosome

mud¹

centrosome | P-stage

mud^f01205

egg & chromatin | maternal effect

mud⁴

egg & spindle | maternal effect

mud⁴

egg | maternal effect

mud⁴

EG neuron | embryonic stage, with Scer\GAL4^eg.PU

mud^EY20197

embryonic/larval brain | third instar larval stage

mud²

embryonic neuroblast

mud^f33

epithelial cell | P-stage

mud^f01205

external sensory organ | P-stage

external sensory organ precursor cell IIa | P-stage

external sensory organ precursor cell IIb | P-stage

ganglion mother cell | somatic clone (with mud³)

mud⁴

ganglion mother cell | somatic clone (with mud⁴)

mud³

Kenyon cell

larval neuroblast

mud¹

larval neuroblast | larval stage

mud²

larval neuroblast | third instar larval stage

mud⁴

lobe system of adult mushroom body

macrochaeta

mud^BG02499

medial lobe of adult mushroom body

mud⁴

mitotic spindle | third instar larval stage

mushroom body pedunculus

mud²

neuroblast | somatic clone | supernumerary (with mud³)

mud⁴

neuroblast | somatic clone | supernumerary (with mud⁴)

mud³

neuroblast | supernumerary (with mud³)

mud⁴

neuroblast | supernumerary (with mud⁴)

mud³

neuroblast | supernumerary | third instar larval stage

mud²

neuropil

mud¹

sensory mother cell | P-stage

SP1 neuron | embryonic stage

mud³

spindle (with mud³)

mud⁴

spindle (with mud⁴)

mud³

spindle | P-stage

tricellular tight junction | P-stage

mud^f01205

ventral nerve cord | embryonic stage

mud³

ventral nerve cord | embryonic stage, with Scer\GAL4^eg.PU

mud^EY20197

ventral nerve cord commissure | embryonic stage

mud³

vertical branch of mushroom body

mud⁴

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v7.1)

Homo sapiens (Human) (15)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\GOLGA4

2 of 15

Yes

Hsap\NUMA1

2 of 15

Yes

Hsap\CENPE

1 of 15

Hsap\CENPF

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Hsap\GOLGB1

1 of 15

Hsap\HMMR

1 of 15

Yes

Hsap\TAX1BP1

1 of 15

Yes

Hsap\TPR

1 of 15

Model Organism Orthologs (via DIOPT v7.1)

Mus musculus (laboratory mouse) (11)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Golga4

2 of 15

Yes

Mmus\Numa1

2 of 15

Yes

Mmus\Cenpe

1 of 15

Mmus\Cenpf

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Mmus\Golgb1

1 of 15

Mmus\Krt17

1 of 15

Yes

Rattus norvegicus (Norway rat) (11)

Rnor\Cenpe

1 of 13

Yes

Rnor\Cenpf

1 of 13

Yes

Rnor\Cep164

1 of 13

Yes

Rnor\Clip1

1 of 13

Yes

Rnor\Clip2

1 of 13

Yes

Rnor\Gcc2

1 of 13

Yes

Rnor\Golga3

1 of 13

Yes

Rnor\Golga4

1 of 13

Yes

Rnor\Golgb1

1 of 13

Yes

Rnor\Numa1

1 of 13

Yes

Rnor\Tpr

1 of 13

Yes

Xenopus tropicalis (Western clawed frog) (1)

Xtro\filip1

1 of 12

Yes

Danio rerio (Zebrafish) (13)

Drer\numa1

3 of 15

Yes

2 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Drer\golgb1

1 of 15

Drer\lamc1

1 of 15

Drer\si:ch211-220f16.2

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Caenorhabditis elegans (Nematode, roundworm) (11)

Cele\che-10

2 of 15

Yes

Cele\golg-4

2 of 15

Yes

Cele\anc-1

1 of 15

Cele\C18C4.7

1 of 15

Yes

Cele\clec-19

1 of 15

Yes

Cele\F59A2.2

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Arabidopsis thaliana (thale-cress) (10)

Atha\AT2G32240

2 of 9

Yes

Atha\CIP1

2 of 9

Yes

Atha\AT3G10180

1 of 9

Atha\AT4G01270

1 of 9

Atha\AT5G40450

1 of 9

Yes

Atha\AT5G41780

1 of 9

Yes

Atha\MFP1

1 of 9

Yes

Atha\NET1A

1 of 9

Yes

Atha\NET1D

1 of 9

Yes

Atha\NUA

1 of 9

Saccharomyces cerevisiae (Brewer's yeast) (3)

Scer\MLP1

2 of 15

Yes

Scer\MLP2

2 of 15

Yes

Scer\USO1

1 of 15

Schizosaccharomyces pombe (Fission yeast) (6)

Spom\spo15

2 of 12

Yes

Spom\alm1

1 of 12

Spom\mto1

1 of 12

Yes

Spom\nup211

1 of 12

Spom\pcp1

1 of 12

Spom\ssm4

1 of 12

Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG0919050D )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Dmel\mud

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA26587

Drosophila persimilis

Dper\GL14926

Drosophila willistoni

Dwil\GK20155

Drosophila virilis

Dvir\GJ23712

Drosophila virilis

Dvir\GJ19063

Drosophila mojavensis

Dmoj\GI15437

Drosophila grimshawi

Dgri\GH12928

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150069 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17015105

Glossina morsitans

Tsetse fly

Gmor\GMOY004998

Glossina morsitans

Tsetse fly

Gmor\GMOY010748

Glossina morsitans

Tsetse fly

Gmor\GMOY010679

Glossina morsitans

Tsetse fly

Gmor\GMOY005738

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_10201

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_10919

Mayetiola destructor

Hessian fly

Mdes\Mdes003252

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL014706

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL002942

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ005801

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ003674

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ017450

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( None identified )

No non-Dipteran orthologies identified

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X004L )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_241908

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( None identified )

No non-Arthropod Metazoa orthologies identified

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v7.1)

Drosophila melanogaster (Fruit fly) (7)

Gene Symbol

Score

Source

Alignment

2 of 10

1 of 10

1 of 10

1 of 10

1 of 10

lva

1 of 10

Mtor

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

NUMA1; nuclear mitotic apparatus protein 1

model of acute promyelocytic leukemia

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

mud - Fs(2)Ket

anti tag coimmunoprecipitation, anti tag western blot

(Wee et al., 2011)

mud - Gαi

anti tag coimmunoprecipitation, anti tag western blot

(Bowman et al., 2006)

mud - Lam

anti bait coimmunoprecipitation, anti tag western blot, affinity chromatography technology, Identification by mass spectrometry

(Capalbo et al., 2011)

mud - Rab5

anti bait coimmunoprecipitation, anti tag western blot, affinity chromatography technology, Identification by mass spectrometry

(Capalbo et al., 2011)

mud - ana2

two hybrid

(Wang et al., 2011)

mud - ctp

anti tag coimmunoprecipitation, western blot, two hybrid

(Wang et al., 2011)

mud - dsh

anti tag coimmunoprecipitation, anti tag western blot

(Ségalen et al., 2010)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

mud

suppressible

pros

(Cabernard and Doe, 2009)

mud

enhanceable

(Campbell and Nash, 2001)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

fra

enhanceable

mud

(Cate et al., 2016)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

69318

DroID - A comprehensive database of gene and protein interactions.

FBgn0002873

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

44839

MIST (protein-protein) - An integrated Molecular Interaction Database

Pathways

Gene Group - Pathway Membership (FlyBase)

External Data

Linkouts

FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel

superoxide radicals degradation

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

1-48

Cytogenetic map

12E5-12E6

Sequence location

X:14,247,732..14,258,595 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

12E5-12E6

Limits computationally determined from genome sequence between P{EP}l(1)G0007^EP764 and P{EP}EP1599

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

12E-12E

(Guan, 1999.7.31)

Experimentally Determined Recombination Data

Location

1-48

(FlyBase, 2016)

1-45.9

(Comeron et al., 2012)

1-50

(Heisenberg, 1980)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (9)

Bloomington

35830

mud¹/FM7a

9562

mud¹/FM7i, P{ActGFP}JMR3

9563

mud⁴/FM7i, P{ActGFP}JMR3

28074

y¹ v¹; P{TRiP.JF02911}attP2

80849

y¹, v¹; P{TKO.GS04658}attP40

22371

y¹ w^67c23 P{EPgy2}mud^EY20197

35044

y¹ sc^* v¹ sev²¹; P{TRiP.HMS01458}attP2

38190

y¹ sc^* v¹ sev²¹; P{TRiP.GL00629}attP40

Harvard

f01205

PBac{WH}mud^f01205

Genomic Clones (11)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (35)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequences

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0002873

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0002873

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

44839

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Izumi et al., 2006)

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for database merge of

Additional comments

Annotation CG12047 split into CG12047 (which corresponds to mud) and CG32599 in release 3 of the genome annotation.

(FlyBase Genome Annotators, 2002-2003)

Other Comments

Expression is enriched in embryonic gonads.

(Shigenobu et al., 2006)

Mutation of mud interferes with the regulation of central nervous system development by affecting the proliferation pattern.

(Prokop and Technau, 1994)

Neuropil of mushroom bodies (usually in dorsal brain) is missing (Heisenberg, 1980); penetrance for this anatomical phenotype (on which criterion the mutant was isolated) ca. 90%. Calyces that should innervate pedunculi and lobes of mushroom bodies are enlarged, as are antennal lobes in anterior brain. At sites of calyces, large numbers of thin axons form distinct lobes outside main neuropil of brain. Viability of mutant females poor and they are sterile as well. Development of mushroom bodies apparently normal until pupation, when 'reconstruction' of this bilaterally paired brain entity (this process being a normal feature of wild-type metamorphosis) is aberrant (Technau and Heisenberg, 1982). Supernumerary neuroblasts observed in the larval brain, owing to anomalous proliferation of such cells postembryonically.

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 71 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

44839

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0002873

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

mud_1

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0002873

Flygut - An atlas of the Drosophila adult midgut

FBgn0002873

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

44839

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG12047

modMine - A data warehouse for the modENCODE project

FBgn0002873

Linkouts

BioGRID - A database of protein and genetic interactions.

69318

DroID - A comprehensive database of gene and protein interactions.

FBgn0002873

DRSC - Results frm RNAi screens

FBgn0002873

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0002873

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG12047-RC;CG12047-RC;CG12047-RA

FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel

superoxide radicals degradation

FlyMine - An integrated database for Drosophila genomics

FBgn0002873

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

44839

MIST (protein-protein) - An integrated Molecular Interaction Database

Synonyms and Secondary IDs (11)

Reported As

Symbol Synonym

CG12047

(Chen et al., 2012, Ni et al., 2011.7.19, Ni et al., 2010.12.1, Zhang et al., 2010, Perkins et al., 2009, Hong et al., 2008, Hughes et al., 2008, Brandt, 2006, Molnar et al., 2006, Giot et al., 2003, Norga et al., 2003, Tzolovsky et al., 2002, Nash, 2000.8.14)

KS63

NuMA

(Osses and Henríquez, 2014, Lu and Prehoda, 2013, Bowman et al., 2006)

NuMa

(Culurgioni and Mapelli, 2013)

mud

(Finegan et al., 2019, Muzzopappa et al., 2017, Transgenic RNAi Project members, 2017-, Tsankova et al., 2017, Cate et al., 2016, John et al., 2016, Bergstralh et al., 2015, Keder et al., 2015, Eliazer et al., 2014, Poulton et al., 2014, Eisman and Kaufman, 2013, Herszterg et al., 2013, Januschke et al., 2013, Jauffred et al., 2013, Nakajima et al., 2013, Saunders et al., 2013, Yoshiura et al., 2012, Wang et al., 2011, Cabernard et al., 2010, Gan et al., 2010, Godin et al., 2010, Wasbrough et al., 2010, Cabernard and Doe, 2009, Cook et al., 2009.11.25, Johnston et al., 2009, Serway et al., 2009, Christensen et al., 2008.9.29, Christensen et al., 2008.12.28, Hong et al., 2008, Hong et al., 2008, Scott, 2008.5.20, Speicher et al., 2008, Cabernard et al., 2007, Nipper et al., 2007, Bowman et al., 2006, Brandt, 2006, Izumi et al., 2006, McIntyre et al., 2006, Molnar et al., 2006, Shigenobu et al., 2006, Siller et al., 2006, Yu et al., 2006, Helfrich-Forster et al., 2002, Guan et al., 2000)

Name Synonyms

Mushroom Body Defect

(Bowman et al., 2006)

Mushroom body defect

(Kelsom and Lu, 2012, Speicher et al., 2008, Bonaccorsi et al., 2007, Speicher et al., 2007, Izumi et al., 2006)

mushroom bodies deranged

(Serway et al., 2009)

mushroom body defect

(Cate et al., 2016, John et al., 2016, Taniguchi et al., 2014, Hong et al., 2008, Helfrich-Forster et al., 2002)

mushroom body defective

(Bajaj et al., 2015, Shen et al., 2013, Cabernard and Doe, 2009, Chia et al., 2008)

Secondary FlyBase IDs

FBgn0052599
FBgn0030557

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (147)

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