mus309, DmBlm, Ku70, Bloom syndrome helicase ortholog, bloom
Gene model reviewed during 5.47
There is only one protein coding transcript and one polypeptide associated with this gene
Monomer. Homodimer (via N-terminus). Homotetramer (via N-terminus); dimer of dimers. Homohexamer (via N-terminus). Self-association negatively regulates DNA unwinding amplitude and rate. Oligomer forms dissociate into monomer in presence of ATP.
The N-terminal region mediates dimerization and homooligomerization. Both the helicase ATP-binding domain and the helicase C-terminal domain form intramolecular interactions with the HRDC domain in a ATP-dependent manner. The HRDC domain is required for single-stranded DNA (ssDNA) and DNA Holliday junction binding.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Blm using the Feature Mapper tool.
GBrowse - Visual display of RNA-Seq signalsView Dmel\Blm in GBrowse 2
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: Blm mus309
Source for merge of: mus309 blm
FlyBase Curator comment: 'mus309' renamed to 'Blm' to reflect the preferred usage in the primary literature (see FBrf0155463, FBrf0216460, FBrf0193899, FBrf0207350, FBrf0107832, FBrf0135945, FBrf0180647, FBrf0200344, FBrf0179341, FBrf0191828, FBrf0202212, FBrf0193858, FBrf0209368). Moreover, symbols with 'mus' prefixes (for 'mutagen-sensitive') are deemed placeholders, to be replaced with a more meaningful symbol when available.
It had previously been suggested that mus309 corresponds to Irbp (FBrf0086898). Detailed deletion mapping suggests that this was incorrect and mus309 actually corresponds to blm, which is supported by the identification of mutations in the blm coding sequence for two mus309 alleles.
mus309 stated to correspond to Irbp. Subsequent information suggests that this is not the case (see Kusano et al., 2001, Science 291(5513): 2600--2602).
mus309 suppresses single strand annealing between divergent repeats (termed homologous SA), thereby preventing potentially deleterious recombination events.
mus309, in collaboration with Top3α can resolve a mobile double Holliday junction substrate through convergent branch migration (in contrast to classic resolvase activities that use a structure-specific endonuclease to cleave the Holliday junctions).
mus309 mutants are severely impaired in their ability to carry out repair DNA synthesis during synthesis-dependent strand annealing in double-strand break repair.
Designation as "DmKu70" was in error.
mus309 gene product is required for the repair of P-element induced double-strand DNA chromosome breaks, both in the presence and absence of a homologous sequence. Plasmid based P-element mobility assay demonstrates that the processing of double strand DNA breaks after P-element excision is severely affected in mus309 mutant embryos.