• Home
• Tools
  • Tools Overview
  • Query Tools

    
    • QuickSearch
    • QueryBuilder
    • TermLink
    • CytoSearch
    • RNA-Seq Search
    • Interactions Browser
    • ImageBrowse
    • Find A Person
    • Google™ FlyBase
  • Genomic/Map Tools

    
    • BLAST
    • GBrowse
    • GBrowse 2 Beta
    • CytoSearch
    • Feature Mapper
    • Aberrations Maps
    • Chromosome Maps
    • Coordinates Converter
  • Retrieve/Convert

    
    • Batch Download
    • Coordinates Converter
    • ID Converter
  • Data Submission

    
    • Fast-Track Your Paper
    • Submit Personal Comm
    • Add A New Person
    • Update Person Info
  • People

    
    • Find A Person
    • Add A New Person
    • Update Person Info
• Files
  • Files Overview
  • Current release
  • Archived releases
  • Map Conversion
  • FTP site:
  • Releases (FTP)
  • Genomes (FTP)
• Species
  • Phylogeny
  • Sequenced Species
  • Synteny Table
  • Color Illustrations
  • Abbreviations
• Documents
  • About FlyBase
  • Reference Manual:
  • Sections
  • Contents
  • Nomenclature
  • Release Notes
• Resources
  • All Resources
  • Model Organisms
  • Stock Collections
  • Vectors & Constructs
  • Other links:
  • BDGP
  • DGRC
  • DIS by issue
  • FlyExpress
  • Interactive Fly
  • modENCODE
  • Textpresso for Fly
• News
  • News
  • FlyBase Forum
  • FlyBase Positions
  • Meetings
  • Courses
  • Fly Board
  • White papers
  • Funding
  • bionet.dros
• Help
  • Google™ FlyBase
  • Site Map
  • FAQs
  • FlyBase Guides
  • Video Tutorials
  • Report help
  • New to Flies
  • Pers. comm.
  • Author guidelines
  • Contact FlyBase
• Archives
  • Prior Release
  • Other Archives
  • Coordinate Converter

Gene Dmel\Blm

General Information
Symbol	Dmel\Blm	Species	D. melanogaster
Name	Bloom syndrome helicase ortholog	Annotation symbol	CG6920
Feature type	protein_coding_gene	FlyBase ID	FBgn0002906
Gene Model Status	Current	Stock availability	12 publicly available
Also Known As	MUS309, DmBlm, RecQ, Ku70
Genomic Location
Chromosome (arm)	3R	Recombination map
Cytogenetic map	86E17-86E17	Sequence location	3R:7,562,532..7,567,505 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Bloom syndrome helicase ortholog is referred to in FlyBase by the symbol Dmel\Blm (CG6920, FBgn0002906). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: Y-form DNA binding; ATP-dependent 3'-5' DNA helicase activity; DNA-dependent ATPase activity. There is experimental evidence that it is involved in the biological process: DNA strand renaturation; reciprocal meiotic recombination; DNA duplex unwinding; negative regulation of double-strand break repair via single-strand annealing; double-strand break repair via synthesis-dependent strand annealing; response to DNA damage stimulus; strand displacement; double-strand break repair; DNA synthesis involved in DNA repair; double-strand break repair via homologous recombination. 17 alleles are reported. The phenotype of these alleles is annotated with: wing disc. It has one annotated transcript and one annotated polypeptide. Protein features are: DNA helicase, ATP-dependent, RecQ type; DNA/RNA helicase, ATP-dependent, DEAH-box type, conserved site; DNA/RNA helicase, DEAD/DEAH box type, N-terminal; HRDC domain; HRDC-like; Helicase, C-terminal; Helicase, superfamily 1/2, ATP-binding domain; RQC domain; Winged helix-turn-helix transcription repressor DNA-binding domain. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of very low expression. Peak expression observed within 00-06 hour embryonic stages, in adult female stages. Summary of FlyAtlas Anatomical Expression Data: Little or no expression detected in any larval or adult organs/tissues. Comments on Affy2 ProbeSet: ProbeSet 1637039_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of mus309. Gene sequence location is 3R:7562532..7567505.
User Contributed Data
	Beta version, feedback welcome
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	mus309 (J.B. Boyd) Larval survival hypersensitive to exposure to methyl methanesulfonate and nitrogen mustard.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 86E17-86E17   Limits computationally determined from genome sequence between P{PZ}tho1&P{PZ}l(3)0462905275 and P{EP}Lk6EP886
Experimentally Determined Cytological Location
Cytogenetic map Notes References 86F1-86F8     (Kusano, 1997.3.7) 86F1-86F8   (determined by in situ hybridisation)   (Kusano et al., 1999)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Blm for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.47 DGC clones appear problematic (GM03260, SD19967): incomplete CDS.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Blm-RA FBtr0082434  NM_079595   4806   1487
Additional Transcript Data & Comments
Reported size (kB)	4.835 (compiled cDNA) (Kusano et al., 1999) 2.4 (northern blot) (Beall et al., 1994) 2.1 (northern blot) (Jacoby and Wensink, 1994)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Blm-PA   FBpp0081910   166.1   1487   8.71     NP_524319   AAF54691
Additional Polypeptide Data & Comments
Reported size (kDa)	1487 (aa) (Kusano et al., 1999) 631 (aa); 69 (kD observed) (Jacoby and Wensink, 1994) 631 (aa); 65-70 (kD observed); 72 (kD predicted) (Beall et al., 1994)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Helicase, C-terminal (IPR001650) HRDC domain (IPR002121) DNA/RNA helicase, ATP-dependent, DEAH-box type, conserved site (IPR002464) DNA helicase, ATP-dependent, RecQ type (IPR004589) DNA/RNA helicase, DEAD/DEAH box type, N-terminal (IPR011545) HRDC-like (IPR010997) Helicase, superfamily 1/2, ATP-binding domain (IPR014001) RQC domain (IPR018982) P-loop containing nucleoside triphosphate hydrolase (IPR027417) Winged helix-turn-helix DNA-binding domain (IPR011991)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA695680   AE014297 AAF54691   AI532788   AY060857 AAL28405   BI637854   BP546271   CO281082   CO282520   CO287639   CO309594   CO339192   CO345033   EC052069   EC052586   EC054045   EC055105   EC055546   EC055751   EC062105   EC075886   EC081462   EC245026   EC257824   EC259740   U92536 AAD41441
UniProtKB/Swiss-Prot	Q9VGI8
UniProtKB/TrEMBL	Q95SC9
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
northern blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage -- adult stage     (Jacoby and Wensink, 1994, Beall et al., 1994)
Additional Descriptive Data
	The Blm transcript is present in all stages, but is most abundant in early embryos and in ovaries. (Jacoby and Wensink, 1994) Blm transcripts are observed at a low level through most of development on northern blots. They are abundant in ovaries, 0-2hr embryos, and adult females. (Beall et al., 1994)
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
nucleus inferred from direct assay (Weinert and Rio, 2007)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Little or no expression detected in any larval or adult organs/tissues. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    8 Larval Midgut    15 Larval Hindgut    8.1 Larval Malpighian Tubules    8.7 Larval Fat Body    18.9 Larval Salivary Gland    16 Larval Trachea    9.475 Larval Carcass    9.675 Adult Head    8.4 Adult Eye    10.85 Adult Brain    9 Adult Thoracic-Abdominal Ganglion    8.5 Adult Crop    10.6 Adult Midgut    13.2 Adult Hindgut    7.3 Adult Malpighian Tubules    6.8 Adult Fat Body    15.8 Adult Salivary Gland    13.3 Adult Heart    12.45 Adult VirginFemale Spermatheca    13.5 Adult InseminatedFemale Spermatheca    11.9 Adult Ovary    9.5 Adult Testis    20.1 Adult Male Accessory Gland    18 Adult Carcass    10.6 Expression Level Scale  None   Low  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    8 Larval Midgut    15 Larval Hindgut    8.1 Larval Malpighian Tubules    8.7 Larval Fat Body    18.9 Larval Salivary Gland    16 Larval Trachea    9.475 Larval Carcass    9.675 Adult Head    8.4 Adult Eye    10.85 Adult Brain    9 Adult Thoracic-Abdominal Ganglion    8.5 Adult Crop    10.6 Adult Midgut    13.2 Adult Hindgut    7.3 Adult Malpighian Tubules    6.8 Adult Fat Body    15.8 Adult Salivary Gland    13.3 Adult Heart    12.45 Adult VirginFemale Spermatheca    13.5 Adult InseminatedFemale Spermatheca    11.9 Adult Ovary    9.5 Adult Testis    20.1 Adult Male Accessory Gland    18 Adult Carcass    10.6 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    8 Larval Midgut    15 Larval Hindgut    8.1 Larval Malpighian Tubules    8.7 Larval Fat Body    18.9 Larval Salivary Gland    16 Larval Trachea    9.475 Larval Carcass    9.675 Adult Head    8.4 Adult Eye    10.85 Adult Brain    9 Adult Thoracic-Abdominal Ganglion    8.5 Adult Crop    10.6 Adult Midgut    13.2 Adult Hindgut    7.3 Adult Malpighian Tubules    6.8 Adult Fat Body    15.8 Adult Salivary Gland    13.3 Adult Heart    12.45 Adult VirginFemale Spermatheca    13.5 Adult InseminatedFemale Spermatheca    11.9 Adult Ovary    9.5 Adult Testis    20.1 Adult Male Accessory Gland    18 Adult Carcass    10.6 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    8 Larval Midgut    15 Larval Hindgut    8.1 Larval Malpighian Tubules    8.7 Larval Fat Body    18.9 Larval Salivary Gland    16 Larval Trachea    9.475 Larval Carcass    9.675 Adult Head    8.4 Adult Eye    10.85 Adult Brain    9 Adult Thoracic-Abdominal Ganglion    8.5 Adult Crop    10.6 Adult Midgut    13.2 Adult Hindgut    7.3 Adult Malpighian Tubules    6.8 Adult Fat Body    15.8 Adult Salivary Gland    13.3 Adult Heart    12.45 Adult VirginFemale Spermatheca    13.5 Adult InseminatedFemale Spermatheca    11.9 Adult Ovary    9.5 Adult Testis    20.1 Adult Male Accessory Gland    18 Adult Carcass    10.6 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    8 Larval Midgut    15 Larval Hindgut    8.1 Larval Malpighian Tubules    8.7 Larval Fat Body    18.9 Larval Salivary Gland    16 Larval Trachea    9.475 Larval Carcass    9.675 Adult Head    8.4 Adult Eye    10.85 Adult Brain    9 Adult Thoracic-Abdominal Ganglion    8.5 Adult Crop    10.6 Adult Midgut    13.2 Adult Hindgut    7.3 Adult Malpighian Tubules    6.8 Adult Fat Body    15.8 Adult Salivary Gland    13.3 Adult Heart    12.45 Adult VirginFemale Spermatheca    13.5 Adult InseminatedFemale Spermatheca    11.9 Adult Ovary    9.5 Adult Testis    20.1 Adult Male Accessory Gland    18 Adult Carcass    10.6 Expression Level Scale  None   Low  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    8 Larval Midgut    15 Larval Hindgut    8.1 Larval Malpighian Tubules    8.7 Larval Fat Body    18.9 Larval Salivary Gland    16 Larval Trachea    9.475 Larval Carcass    9.675 Adult Head    8.4 Adult Eye    10.85 Adult Brain    9 Adult Thoracic-Abdominal Ganglion    8.5 Adult Crop    10.6 Adult Midgut    13.2 Adult Hindgut    7.3 Adult Malpighian Tubules    6.8 Adult Fat Body    15.8 Adult Salivary Gland    13.3 Adult Heart    12.45 Adult VirginFemale Spermatheca    13.5 Adult InseminatedFemale Spermatheca    11.9 Adult Ovary    9.5 Adult Testis    20.1 Adult Male Accessory Gland    18 Adult Carcass    10.6 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    8 Larval Midgut    15 Larval Hindgut    8.1 Larval Malpighian Tubules    8.7 Larval Fat Body    18.9 Larval Salivary Gland    16 Larval Trachea    9.475 Larval Carcass    9.675 Adult Head    8.4 Adult Eye    10.85 Adult Brain    9 Adult Thoracic-Abdominal Ganglion    8.5 Adult Crop    10.6 Adult Midgut    13.2 Adult Hindgut    7.3 Adult Malpighian Tubules    6.8 Adult Fat Body    15.8 Adult Salivary Gland    13.3 Adult Heart    12.45 Adult VirginFemale Spermatheca    13.5 Adult InseminatedFemale Spermatheca    11.9 Adult Ovary    9.5 Adult Testis    20.1 Adult Male Accessory Gland    18 Adult Carcass    10.6 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    8 Larval Midgut    15 Larval Hindgut    8.1 Larval Malpighian Tubules    8.7 Larval Fat Body    18.9 Larval Salivary Gland    16 Larval Trachea    9.475 Larval Carcass    9.675 Adult Head    8.4 Adult Eye    10.85 Adult Brain    9 Adult Thoracic-Abdominal Ganglion    8.5 Adult Crop    10.6 Adult Midgut    13.2 Adult Hindgut    7.3 Adult Malpighian Tubules    6.8 Adult Fat Body    15.8 Adult Salivary Gland    13.3 Adult Heart    12.45 Adult VirginFemale Spermatheca    13.5 Adult InseminatedFemale Spermatheca    11.9 Adult Ovary    9.5 Adult Testis    20.1 Adult Male Accessory Gland    18 Adult Carcass    10.6 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    8.4   NA  Eye    10.85   NA  Brain    9   8  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    8.5   NA  Crop    no informative data   15  Midgut    no informative data   8.1  Hindgut    7.3   8.7  Malpighian Tubules    6.8   no informative data  Fat Body    15.8   no informative data  Salivary Gland    no informative data   NA  Heart    no informative data   9.475  Trachea    NA   NA  VirginFemale Spermatheca    no informative data   NA  InseminatedFemale Spermatheca    no informative data   NA  Ovary    9.5   NA  Testis    20.1   NA  Male Accessory Gland    no informative data   9.675  Carcass    no informative data FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    13 central nervous system, larvae L3    6 central nervous system, pupae P8    3 head, virgin 1-day female    2 head, virgin 4-day female    1 head, virgin 20-day female    1 head, mated 1-day female    1 head, mated 4-day female    1 head, mated 20-day female    1 head, mated 1-day male    2 head, mated 4-day male    2 head, mated 20-day male    3 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    1 digestive system, 4-day adult    2 digestive system, 20-day adult    1 fat body, larvae L3 wandering    1 fat body, white prepupae    4 fat body, pupae P8    1 carcass, larvae L3 wandering    4 carcass, 1-day adult    2 carcass, 4-day adult    2 carcass, 20-day adult    2 ovary, virgin 4-day female    44 ovary, mated 4-day female    37 testis, mated 4-day male    13 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    13 central nervous system, larvae L3    6 central nervous system, pupae P8    3 head, virgin 1-day female    2 head, virgin 4-day female    1 head, virgin 20-day female    1 head, mated 1-day female    1 head, mated 4-day female    1 head, mated 20-day female    1 head, mated 1-day male    2 head, mated 4-day male    2 head, mated 20-day male    3 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    1 digestive system, 4-day adult    2 digestive system, 20-day adult    1 fat body, larvae L3 wandering    1 fat body, white prepupae    4 fat body, pupae P8    1 carcass, larvae L3 wandering    4 carcass, 1-day adult    2 carcass, 4-day adult    2 carcass, 20-day adult    2 ovary, virgin 4-day female  (44) ovary, mated 4-day female  (37) testis, mated 4-day male    13 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    13 central nervous system, larvae L3    6 central nervous system, pupae P8    3 head, virgin 1-day female    2 head, virgin 4-day female    1 head, virgin 20-day female    1 head, mated 1-day female    1 head, mated 4-day female    1 head, mated 20-day female    1 head, mated 1-day male    2 head, mated 4-day male    2 head, mated 20-day male    3 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    1 digestive system, 4-day adult    2 digestive system, 20-day adult    1 fat body, larvae L3 wandering    1 fat body, white prepupae    4 fat body, pupae P8    1 carcass, larvae L3 wandering    4 carcass, 1-day adult    2 carcass, 4-day adult    2 carcass, 20-day adult    2 ovary, virgin 4-day female    44 ovary, mated 4-day female    37 testis, mated 4-day male    13 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    13 central nervous system, larvae L3    6 central nervous system, pupae P8    3 head, virgin 1-day female    2 head, virgin 4-day female    1 head, virgin 20-day female    1 head, mated 1-day female    1 head, mated 4-day female    1 head, mated 20-day female    1 head, mated 1-day male    2 head, mated 4-day male    2 head, mated 20-day male    3 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    1 digestive system, 4-day adult    2 digestive system, 20-day adult    1 fat body, larvae L3 wandering    1 fat body, white prepupae    4 fat body, pupae P8    1 carcass, larvae L3 wandering    4 carcass, 1-day adult    2 carcass, 4-day adult    2 carcass, 20-day adult    2 ovary, virgin 4-day female    44 ovary, mated 4-day female    37 testis, mated 4-day male    13 accessory gland, mated 4-day male    3 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    13 central nervous system, larvae L3    6 central nervous system, pupae P8    3 head, virgin 1-day female    2 head, virgin 4-day female    1 head, virgin 20-day female    1 head, mated 1-day female    1 head, mated 4-day female    1 head, mated 20-day female    1 head, mated 1-day male    2 head, mated 4-day male    2 head, mated 20-day male    3 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    1 digestive system, 4-day adult    2 digestive system, 20-day adult    1 fat body, larvae L3 wandering    1 fat body, white prepupae    4 fat body, pupae P8    1 carcass, larvae L3 wandering    4 carcass, 1-day adult    2 carcass, 4-day adult    2 carcass, 20-day adult    2 ovary, virgin 4-day female    44 ovary, mated 4-day female    37 testis, mated 4-day male    13 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    13 central nervous system, larvae L3    6 central nervous system, pupae P8    3 head, virgin 1-day female    2 head, virgin 4-day female    1 head, virgin 20-day female    1 head, mated 1-day female    1 head, mated 4-day female    1 head, mated 20-day female    1 head, mated 1-day male    2 head, mated 4-day male    2 head, mated 20-day male    3 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    1 digestive system, 4-day adult    2 digestive system, 20-day adult    1 fat body, larvae L3 wandering    1 fat body, white prepupae    4 fat body, pupae P8    1 carcass, larvae L3 wandering    4 carcass, 1-day adult    2 carcass, 4-day adult    2 carcass, 20-day adult    2 ovary, virgin 4-day female  (44) ovary, mated 4-day female  (37) testis, mated 4-day male    13 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    13 central nervous system, larvae L3    6 central nervous system, pupae P8    3 head, virgin 1-day female    2 head, virgin 4-day female    1 head, virgin 20-day female    1 head, mated 1-day female    1 head, mated 4-day female    1 head, mated 20-day female    1 head, mated 1-day male    2 head, mated 4-day male    2 head, mated 20-day male    3 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    1 digestive system, 4-day adult    2 digestive system, 20-day adult    1 fat body, larvae L3 wandering    1 fat body, white prepupae    4 fat body, pupae P8    1 carcass, larvae L3 wandering    4 carcass, 1-day adult    2 carcass, 4-day adult    2 carcass, 20-day adult    2 ovary, virgin 4-day female    44 ovary, mated 4-day female    37 testis, mated 4-day male    13 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    13 central nervous system, larvae L3    6 central nervous system, pupae P8    3 head, virgin 1-day female    2 head, virgin 4-day female    1 head, virgin 20-day female    1 head, mated 1-day female    1 head, mated 4-day female    1 head, mated 20-day female    1 head, mated 1-day male    2 head, mated 4-day male    2 head, mated 20-day male    3 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    1 digestive system, 4-day adult    2 digestive system, 20-day adult    1 fat body, larvae L3 wandering    1 fat body, white prepupae    4 fat body, pupae P8    1 carcass, larvae L3 wandering    4 carcass, 1-day adult    2 carcass, 4-day adult    2 carcass, 20-day adult    2 ovary, virgin 4-day female    44 ovary, mated 4-day female    37 testis, mated 4-day male    13 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of very low expression. Peak expression observed within 00-06 hour embryonic stages, in adult female stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    28 embryo 02-04hr    6 embryo 04-06hr    10 embryo 06-08hr    9 embryo 08-10hr    6 embryo 10-12hr    2 embryo 12-14hr    2 embryo 14-16hr    1 embryo 16-18hr    1 embryo 18-20hr    1 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    2 larva L2    2 larva L3 12hr old    1 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    3 larva L3 puffstage 7-9    4 white prepupae new    4 white prepupae 12hr    4 white prepupae 24hr    4 pupae 2d postWPP    3 pupae 3d postWPP    2 pupae 4d postWPP    1 adult male 01day    2 adult male 05day    3 adult male 30day    3 adult female 01day    7 adult female 05day    20 adult female 30day    18 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (28) embryo 02-04hr    6 embryo 04-06hr    10 embryo 06-08hr    9 embryo 08-10hr    6 embryo 10-12hr    2 embryo 12-14hr    2 embryo 14-16hr    1 embryo 16-18hr    1 embryo 18-20hr    1 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    2 larva L2    2 larva L3 12hr old    1 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    3 larva L3 puffstage 7-9    4 white prepupae new    4 white prepupae 12hr    4 white prepupae 24hr    4 pupae 2d postWPP    3 pupae 3d postWPP    2 pupae 4d postWPP    1 adult male 01day    2 adult male 05day    3 adult male 30day    3 adult female 01day    7 adult female 05day    20 adult female 30day    18 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    28 embryo 02-04hr    6 embryo 04-06hr    10 embryo 06-08hr    9 embryo 08-10hr    6 embryo 10-12hr    2 embryo 12-14hr    2 embryo 14-16hr    1 embryo 16-18hr    1 embryo 18-20hr    1 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    2 larva L2    2 larva L3 12hr old    1 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    3 larva L3 puffstage 7-9    4 white prepupae new    4 white prepupae 12hr    4 white prepupae 24hr    4 pupae 2d postWPP    3 pupae 3d postWPP    2 pupae 4d postWPP    1 adult male 01day    2 adult male 05day    3 adult male 30day    3 adult female 01day    7 adult female 05day    20 adult female 30day    18 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    28 embryo 02-04hr    6 embryo 04-06hr    10 embryo 06-08hr    9 embryo 08-10hr    6 embryo 10-12hr    2 embryo 12-14hr    2 embryo 14-16hr    1 embryo 16-18hr    1 embryo 18-20hr    1 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    2 larva L2    2 larva L3 12hr old    1 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    3 larva L3 puffstage 7-9    4 white prepupae new    4 white prepupae 12hr    4 white prepupae 24hr    4 pupae 2d postWPP    3 pupae 3d postWPP    2 pupae 4d postWPP    1 adult male 01day    2 adult male 05day    3 adult male 30day    3 adult female 01day    7 adult female 05day    20 adult female 30day    18 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    28 embryo 02-04hr    6 embryo 04-06hr    10 embryo 06-08hr    9 embryo 08-10hr    6 embryo 10-12hr    2 embryo 12-14hr    2 embryo 14-16hr    1 embryo 16-18hr    1 embryo 18-20hr    1 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    2 larva L2    2 larva L3 12hr old    1 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    3 larva L3 puffstage 7-9    4 white prepupae new    4 white prepupae 12hr    4 white prepupae 24hr    4 pupae 2d postWPP    3 pupae 3d postWPP    2 pupae 4d postWPP    1 adult male 01day    2 adult male 05day    3 adult male 30day    3 adult female 01day    7 adult female 05day    20 adult female 30day    18 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  28 embryo 02-04hr    6 embryo 04-06hr    10 embryo 06-08hr    9 embryo 08-10hr    6 embryo 10-12hr    2 embryo 12-14hr    2 embryo 14-16hr    1 embryo 16-18hr    1 embryo 18-20hr    1 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    2 larva L2    2 larva L3 12hr old    1 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    3 larva L3 puffstage 7-9    4 white prepupae new    4 white prepupae 12hr    4 white prepupae 24hr    4 pupae 2d postWPP    3 pupae 3d postWPP    2 pupae 4d postWPP    1 adult male 01day    2 adult male 05day    3 adult male 30day    3 adult female 01day    7 adult female 05day    20 adult female 30day    18 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    28 embryo 02-04hr    6 embryo 04-06hr    10 embryo 06-08hr    9 embryo 08-10hr    6 embryo 10-12hr    2 embryo 12-14hr    2 embryo 14-16hr    1 embryo 16-18hr    1 embryo 18-20hr    1 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    2 larva L2    2 larva L3 12hr old    1 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    3 larva L3 puffstage 7-9    4 white prepupae new    4 white prepupae 12hr    4 white prepupae 24hr    4 pupae 2d postWPP    3 pupae 3d postWPP    2 pupae 4d postWPP    1 adult male 01day    2 adult male 05day    3 adult male 30day    3 adult female 01day    7 adult female 05day    20 adult female 30day    18 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    28 embryo 02-04hr    6 embryo 04-06hr    10 embryo 06-08hr    9 embryo 08-10hr    6 embryo 10-12hr    2 embryo 12-14hr    2 embryo 14-16hr    1 embryo 16-18hr    1 embryo 18-20hr    1 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    2 larva L2    2 larva L3 12hr old    1 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    3 larva L3 puffstage 7-9    4 white prepupae new    4 white prepupae 12hr    4 white prepupae 24hr    4 pupae 2d postWPP    3 pupae 3d postWPP    2 pupae 4d postWPP    1 adult male 01day    2 adult male 05day    3 adult male 30day    3 adult female 01day    7 adult female 05day    20 adult female 30day    18 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    13 Schneider line 2 Sg4    4 embryonic 1182-4H    8 embryonic GM2    7 embryonic Kc167    8 embryonic S1    7 embryonic S3    11 leg disc CME L1    6 wing disc CME-W2    10 wing disc ML-DmD8    7 wing disc ML-DmD9    7 wing disc ML-DmD16-c3    7 wing disc ML-DmD21    3 wing disc ML-DmD32    8 haltere disc ML-DmD17-c3    14 eye-antennal disc ML-DmD11    7 antennal disc ML-DmD20-c5    4 mixed discs ML-DmD4-c1    6 CNS ML-DmBG1-c1    13 CNS ML-DmBG2-c2    7 tumorous blood cells mbn2    5 ovary fGS/OSS    17 ovary OSC    9 ovary OSS    8 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    13 Schneider line 2 Sg4    4 embryonic 1182-4H    8 embryonic GM2    7 embryonic Kc167    8 embryonic S1    7 embryonic S3    11 leg disc CME L1    6 wing disc CME-W2    10 wing disc ML-DmD8    7 wing disc ML-DmD9    7 wing disc ML-DmD16-c3    7 wing disc ML-DmD21    3 wing disc ML-DmD32    8 haltere disc ML-DmD17-c3    14 eye-antennal disc ML-DmD11    7 antennal disc ML-DmD20-c5    4 mixed discs ML-DmD4-c1    6 CNS ML-DmBG1-c1    13 CNS ML-DmBG2-c2    7 tumorous blood cells mbn2    5 ovary fGS/OSS    17 ovary OSC    9 ovary OSS    8 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    13 Schneider line 2 Sg4    4 embryonic 1182-4H    8 embryonic GM2    7 embryonic Kc167    8 embryonic S1    7 embryonic S3    11 leg disc CME L1    6 wing disc CME-W2    10 wing disc ML-DmD8    7 wing disc ML-DmD9    7 wing disc ML-DmD16-c3    7 wing disc ML-DmD21    3 wing disc ML-DmD32    8 haltere disc ML-DmD17-c3    14 eye-antennal disc ML-DmD11    7 antennal disc ML-DmD20-c5    4 mixed discs ML-DmD4-c1    6 CNS ML-DmBG1-c1    13 CNS ML-DmBG2-c2    7 tumorous blood cells mbn2    5 ovary fGS/OSS    17 ovary OSC    9 ovary OSS    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    13 Schneider line 2 Sg4    4 embryonic 1182-4H    8 embryonic GM2    7 embryonic Kc167    8 embryonic S1    7 embryonic S3    11 leg disc CME L1    6 wing disc CME-W2    10 wing disc ML-DmD8    7 wing disc ML-DmD9    7 wing disc ML-DmD16-c3    7 wing disc ML-DmD21    3 wing disc ML-DmD32    8 haltere disc ML-DmD17-c3    14 eye-antennal disc ML-DmD11    7 antennal disc ML-DmD20-c5    4 mixed discs ML-DmD4-c1    6 CNS ML-DmBG1-c1    13 CNS ML-DmBG2-c2    7 tumorous blood cells mbn2    5 ovary fGS/OSS    17 ovary OSC    9 ovary OSS    8 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    13 Schneider line 2 Sg4    4 embryonic 1182-4H    8 embryonic GM2    7 embryonic Kc167    8 embryonic S1    7 embryonic S3    11 leg disc CME L1    6 wing disc CME-W2    10 wing disc ML-DmD8    7 wing disc ML-DmD9    7 wing disc ML-DmD16-c3    7 wing disc ML-DmD21    3 wing disc ML-DmD32    8 haltere disc ML-DmD17-c3    14 eye-antennal disc ML-DmD11    7 antennal disc ML-DmD20-c5    4 mixed discs ML-DmD4-c1    6 CNS ML-DmBG1-c1    13 CNS ML-DmBG2-c2    7 tumorous blood cells mbn2    5 ovary fGS/OSS    17 ovary OSC    9 ovary OSS    8 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    13 Schneider line 2 Sg4    4 embryonic 1182-4H    8 embryonic GM2    7 embryonic Kc167    8 embryonic S1    7 embryonic S3    11 leg disc CME L1    6 wing disc CME-W2    10 wing disc ML-DmD8    7 wing disc ML-DmD9    7 wing disc ML-DmD16-c3    7 wing disc ML-DmD21    3 wing disc ML-DmD32    8 haltere disc ML-DmD17-c3    14 eye-antennal disc ML-DmD11    7 antennal disc ML-DmD20-c5    4 mixed discs ML-DmD4-c1    6 CNS ML-DmBG1-c1    13 CNS ML-DmBG2-c2    7 tumorous blood cells mbn2    5 ovary fGS/OSS    17 ovary OSC    9 ovary OSS    8 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    13 Schneider line 2 Sg4    4 embryonic 1182-4H    8 embryonic GM2    7 embryonic Kc167    8 embryonic S1    7 embryonic S3    11 leg disc CME L1    6 wing disc CME-W2    10 wing disc ML-DmD8    7 wing disc ML-DmD9    7 wing disc ML-DmD16-c3    7 wing disc ML-DmD21    3 wing disc ML-DmD32    8 haltere disc ML-DmD17-c3    14 eye-antennal disc ML-DmD11    7 antennal disc ML-DmD20-c5    4 mixed discs ML-DmD4-c1    6 CNS ML-DmBG1-c1    13 CNS ML-DmBG2-c2    7 tumorous blood cells mbn2    5 ovary fGS/OSS    17 ovary OSC    9 ovary OSS    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    13 Schneider line 2 Sg4    4 embryonic 1182-4H    8 embryonic GM2    7 embryonic Kc167    8 embryonic S1    7 embryonic S3    11 leg disc CME L1    6 wing disc CME-W2    10 wing disc ML-DmD8    7 wing disc ML-DmD9    7 wing disc ML-DmD16-c3    7 wing disc ML-DmD21    3 wing disc ML-DmD32    8 haltere disc ML-DmD17-c3    14 eye-antennal disc ML-DmD11    7 antennal disc ML-DmD20-c5    4 mixed discs ML-DmD4-c1    6 CNS ML-DmBG1-c1    13 CNS ML-DmBG2-c2    7 tumorous blood cells mbn2    5 ovary fGS/OSS    17 ovary OSC    9 ovary OSS    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    7 cold shock, 4-day adult    5 heat shock, 4-day adult    9 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    9 Cadmium 100 mM 48 hrs, 4-day adult    27 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    16 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    8 Ethanol 2.5% 3 hrs, larvae L3    24 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    26 Caffeine 25 mg/ml 48 hrs, 4-day adult    9 Paraquat 5 mM 48 hrs, 4-day adult    19 Paraquat 10 mM 48 hrs, 4-day adult    14 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    7 cold shock, 4-day adult    5 heat shock, 4-day adult    9 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    9 Cadmium 100 mM 48 hrs, 4-day adult  (27) Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    16 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    8 Ethanol 2.5% 3 hrs, larvae L3    24 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  26 Caffeine 25 mg/ml 48 hrs, 4-day adult    9 Paraquat 5 mM 48 hrs, 4-day adult    19 Paraquat 10 mM 48 hrs, 4-day adult    14 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    7 cold shock, 4-day adult    5 heat shock, 4-day adult    9 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    9 Cadmium 100 mM 48 hrs, 4-day adult    27 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    16 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    8 Ethanol 2.5% 3 hrs, larvae L3    24 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    26 Caffeine 25 mg/ml 48 hrs, 4-day adult    9 Paraquat 5 mM 48 hrs, 4-day adult    19 Paraquat 10 mM 48 hrs, 4-day adult    14 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    7 cold shock, 4-day adult    5 heat shock, 4-day adult    9 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    9 Cadmium 100 mM 48 hrs, 4-day adult    27 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    16 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    8 Ethanol 2.5% 3 hrs, larvae L3    24 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    26 Caffeine 25 mg/ml 48 hrs, 4-day adult    9 Paraquat 5 mM 48 hrs, 4-day adult    19 Paraquat 10 mM 48 hrs, 4-day adult    14 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    7 cold shock, 4-day adult    5 heat shock, 4-day adult    9 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    9 Cadmium 100 mM 48 hrs, 4-day adult    27 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    16 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    8 Ethanol 2.5% 3 hrs, larvae L3    24 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    26 Caffeine 25 mg/ml 48 hrs, 4-day adult    9 Paraquat 5 mM 48 hrs, 4-day adult    19 Paraquat 10 mM 48 hrs, 4-day adult    14 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    7 cold shock, 4-day adult    5 heat shock, 4-day adult    9 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    9 Cadmium 100 mM 48 hrs, 4-day adult  27 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    16 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    8 Ethanol 2.5% 3 hrs, larvae L3    24 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  26 Caffeine 25 mg/ml 48 hrs, 4-day adult    9 Paraquat 5 mM 48 hrs, 4-day adult    19 Paraquat 10 mM 48 hrs, 4-day adult    14 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    7 cold shock, 4-day adult    5 heat shock, 4-day adult    9 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    9 Cadmium 100 mM 48 hrs, 4-day adult    27 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    16 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    8 Ethanol 2.5% 3 hrs, larvae L3    24 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    26 Caffeine 25 mg/ml 48 hrs, 4-day adult    9 Paraquat 5 mM 48 hrs, 4-day adult    19 Paraquat 10 mM 48 hrs, 4-day adult    14 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    7 cold shock, 4-day adult    5 heat shock, 4-day adult    9 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    9 Cadmium 100 mM 48 hrs, 4-day adult    27 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    16 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    8 Ethanol 2.5% 3 hrs, larvae L3    24 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    26 Caffeine 25 mg/ml 48 hrs, 4-day adult    9 Paraquat 5 mM 48 hrs, 4-day adult    19 Paraquat 10 mM 48 hrs, 4-day adult    14 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_06 mE1_20_mRNA_expression_cluster_06
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0002906 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG6920-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | embryonic stage | maternal effect BlmN1 lethal | embryonic stage | maternal effect (with BlmD2) BlmD3 BlmN1 lethal | embryonic stage | maternal effect (with BlmD3) BlmD2 BlmN1 lethal | embryonic stage | maternal effect (with BlmN1) BlmD2 BlmD3 lethal | embryonic stage | maternal effect | partially BlmN2 BlmN3 BlmN4 viable BlmD2 BlmEY03745 viable, with Scer\GAL4pnr-MD237 BlmGD4512 Sterility Allele female sterile (with BlmD2) BlmD3 female sterile (with BlmD3) BlmD2 female sterile (with Df(3R)T-7) BlmD2 female sterile | recessive BlmD2 BlmD3 fertile BlmEY03745 male sterile | dominant | partially BlmD2 male sterile | partially (with Df(3R)T-7) BlmD2 Other Phenotypes Allele chemical sensitive (with BlmD2) BlmD3 chemical sensitive (with BlmD3) BlmD2 chemical sensitive (with Df(3R)T-7) BlmD2 BlmD3 chemical sensitive | recessive BlmD1 BlmD2 BlmD3 chemical sensitive | semidominant BlmD2 DNA repair defective BlmD2 BlmD3 increased cell death BlmN1 radiation sensitive (with BlmD2) BlmD3 radiation sensitive (with BlmD3) BlmD2 radiation sensitive | recessive BlmD2 BlmD3 BlmN1 BlmN2 wild-type Blmhs.PK Phenotype manifest in Allele syncytial blastoderm embryo | maternal effect Blmunspecified wing disc BlmN1
Classical Alleles ( 10 )
For All Classical Alleles Show
Allele of Blm Class Mutagen Stocks Known lesion BlmD2 ethyl methanesulfonate 3 Yes BlmCB-6376-3 1 -- BlmD3 ethyl methanesulfonate 1 Yes BlmEY03745 P-element activity 1 -- BlmN1 P-element activity 1 Yes BlmN2 P-element activity 1 Yes BlmD1 ethyl methanesulfonate 0 -- BlmN3 P-element activity 0 Yes BlmN4 P-element activity 0 Yes Blmunspecified 0 --
Alleles Carried on Transgenic Constructs ( 7 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Blm Class Mutagen Stocks Known lesion BlmGD4512 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes BlmJF01287 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes BlmJF01288 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes BlmdsRNA.cMa in vitro construct - RNAi 0 Yes BlmgBLM in vitro construct - genomic fragment 0 Yes Blmhs.PK in vitro construct - regulatory fusion 0 Yes BlmScer\UAS.cSa in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3R)E79 (Szabad, 1998.4.8) Df(3R)Exel8154 (Ryder, 2004.4.26) Df(3R)kar-D3 (Szabad, 1998.4.8) Df(3R)M-Kx1 (Szabad, 1998.4.8) Df(3R)P-10 (Szabad, 1998.4.8) Df(3R)P-21 (Szabad, 1998.4.8) Df(3R)pros235 (Kusano et al., 2001) Df(3R)T-7 (Szabad, 1998.4.8, Kusano et al., 2001) Df(3R)T-10 (Szabad, 1998.4.8) Df(3R)T-32 (Szabad, 1998.4.8) Df(3R)T-45 (Szabad, 1998.4.8) Df(3R)T-47 (Szabad, 1998.4.8)
Not disrupted in	Df(3R)cu (Szabad, 1998.4.8) Df(3R)E229 (Szabad, 1998.4.8) Df(3R)kar-D1 (Szabad, 1998.4.8) Df(3R)kar-H10 (Szabad, 1998.4.8) Df(3R)kar-Sz12 (Szabad, 1998.4.8) Df(3R)M86D (Szabad, 1998.4.8) Df(3R)pros640 (Kusano et al., 2001) Df(3R)thoR1 (Kusano et al., 2001)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EPgy2}BlmEY03745 NA miscellaneous insertions P{RS3}BlmCB-6376-3 NA insertion of enhancer trap P{lacW}l(3)S044402S044402 No
Gene Ontology: Function, Process & Cellular Component ( 19 unique terms )
Terms Based on Experimental Evidence ( 14 terms )
Molecular Function
CV term Evidence References
ATP-dependent 3'-5' DNA helicase activity inferred from direct assay (Weinert and Rio, 2007) DNA-dependent ATPase activity inferred from direct assay (Weinert and Rio, 2007) Y-form DNA binding inferred from direct assay (Weinert and Rio, 2007)
Biological Process
CV term Evidence References
DNA duplex unwinding inferred from direct assay (Weinert and Rio, 2007) DNA strand renaturation inferred from direct assay (Weinert and Rio, 2007) DNA synthesis involved in DNA repair inferred from mutant phenotype (Adams et al., 2003) double-strand break repair inferred from mutant phenotype (Kusano et al., 2001, Beall and Rio, 1996) double-strand break repair via homologous recombination inferred from mutant phenotype (Johnson-Schlitz and Engels, 2006) double-strand break repair via synthesis-dependent strand annealing inferred from mutant phenotype (McVey et al., 2007, McVey et al., 2004) negative regulation of double-strand break repair via single-strand annealing inferred from mutant phenotype (Kappeler et al., 2008) reciprocal meiotic recombination inferred from mutant phenotype (McVey et al., 2007) response to DNA damage stimulus inferred from genetic interaction with Saccharomyces SGS1 (Kusano et al., 1999) inferred from mutant phenotype (McVey et al., 2007) strand displacement inferred from direct assay (Weinert and Rio, 2007)
Cellular Component
CV term Evidence References
nucleus inferred from direct assay (Weinert and Rio, 2007)
Terms Based on Predictions or Assertions ( 5 terms )
Molecular Function
CV term Evidence References
ATP binding inferred from electronic annotation with InterPro:IPR001650, InterPro:IPR002464, InterPro:IPR011545 (FlyBase Curators et al., 2004-) ATP-dependent DNA helicase activity inferred from sequence or structural similarity with human BLM (Kusano et al., 1999) DNA helicase activity inferred from sequence or structural similarity with human BLM (Sekelsky et al., 2000) helicase activity inferred from sequence or structural similarity (Lasko, 2000)
Biological Process
CV term Evidence References
DNA replication inferred from electronic annotation with InterPro:IPR018982 (FlyBase Curators et al., 2004-)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Blm allele Gene References mus309D2 Irbp (Beall and Rio, 1996, Kusano et al., 2001) okr (Kooistra et al., 1999) mus309D3 Irbp (Beall and Rio, 1996) okr (Kooistra et al., 1999)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 66499 DroID - A comprehensive database of gene and protein interactions. • FBgn0002906 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 41366
Orthologs
OrthoDB Orthologs (45) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6K6GPK Dipteran inclusive ortholog search EOG6PCB12 Insect inclusive ortholog search EOG6280WH Arthropod inclusive ortholog search EOG65X6B0 Metazoa inclusive ortholog search EOG622PBK
Orthologs in Drosophila Species (EOG6K6GPK)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Blm     Drosophila simulans   Dsim\GD20640 Y   Drosophila sechellia   Dsec\GM26077 Y   Drosophila erecta   Dere\GG17198 Y   Drosophila yakuba   Dyak\GE10090 Y   Drosophila ananassae   Dana\GF16620 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA19957 Y   Drosophila persimilis   Dper\GL12253 Y   Drosophila willistoni   Dwil\GK13825 Y   Drosophila virilis   Dvir\GJ10048 Y   Drosophila mojavensis   Dmoj\GI22449 Y   Drosophila grimshawi   Dgri\GH17364 Y
Orthologs in non-Drosophila Dipterans (EOG6PCB12)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL004039   Anopheles gambiae Malaria mosquito  Agam\AGAP002967   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ007633
Orthologs in non-Dipteran Insects (EOG6280WH)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB48074   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG008577   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG003348   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG013991   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH14619   Atta cephalotes Leafcutter ant  Acep\ACEP11919   Camponotus floridanus Florida carpenter ant  Cflo\CFLO15061   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL14872   Linepithema humile Argentine ant  Lhum\LH13834   Pogonomyrmex barbatus Red harvester ant  Pbar\PB11182   Pogonomyrmex barbatus Red harvester ant  Pbar\PB20414   Pogonomyrmex barbatus Red harvester ant  Pbar\PB11179   Solenopsis invicta Red fire ant  Sinv\SINV12262   Acyrthosiphon pisum Pea aphid  Apim\ACYPI002638   Acyrthosiphon pisum Pea aphid  Apim\ACYPI005125   Acyrthosiphon pisum Pea aphid  Apim\ACYPI006034   Bombyx mori Silkmoth  Bmor\BGIBMGA012404   Pediculus humanus Human body louse  Phum\PHUM308920   Tribolium castaneum Red flour beetle  Tcas\TC010005
Orthologs in non-Insect Arthropods (EOG65X6B0)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\JGI_V11_347587   Ixodes scapularis Deer tick  Isca\ISCW004241
Orthologs in non-Arthropod Metazoa (EOG622PBK)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\him-6   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_025393gn   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_009448gn   Danio rerio Zebrafish  Drer\OTTDARG00000029664   Xenopus tropicalis Western clawed frog  Xtro\blm   Gallus gallus Domestic chicken  Ggal\BLM   Mus musculus House mouse  Mmus\Blm   Rattus norvegicus Norway rat  Rnor\ENSRNOG00000011213   Homo sapiens Human  Hsap\BLM
Human Orthologs (1)
Gene OMIM HGNC Hsap\BLM
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD20640 Drosophila sechellia Dsec\GM26077 Drosophila erecta Dere\GG17198 Drosophila yakuba Dyak\GE10090 Drosophila ananassae Dana\GF16620 Drosophila pseudoobscura pseudoobscura Dpse\GA19957 Drosophila persimilis Dper\GL12253 Drosophila willistoni Dwil\GK13825 Drosophila virilis Dvir\GJ10048 Drosophila mojavensis Dmoj\GI22449 Drosophila grimshawi Dgri\GH17364
Stocks & Reagents
Stocks Listed in FlyBase ( 12 )
Bloomington	31331 y1 v1; P{TRiP.JF01288}attP2 28878 w1118; BlmN1/TM3, Sb1 15911 y1 w67c23; P{EPgy2}BlmEY03745 8656 BlmD3 ry506/TM3, Sb1 ryRK 31330 y1 v1; P{TRiP.JF01287}attP2/TM3, Ser1 8657 st1 BlmD2 Sb1 P{Δ2-3}99B/TM6B, Tb1 28879 y1/Dp(1;Y)y+; BlmN2 ry506 Sb1 P{Δ2-3}99B/TM6B, Tb1
Kyoto	124079 w1118; P{RS3}BlmCB-6376-3 101850 w1118 ; P{lacW}BlmD2 / TM3, Sb1 101851 w1118; P{lacW}BlmD2 P{Delta2-3} / TM3, Sb1
VDRC	v13309 w1118; P{GD4512}v13309 v13310 w1118; P{GD4512}v13310/TM3
Genomic Clones ( 1 )
	BACR11G22 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 22 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GM03260
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	SD19967
Other clones	37657 45098 83747 214247.52 214565.46 CK01.83A.D8 EC052069 EC052586 EC054045 EC055105 EC055546 EC055751 EK066348 EK155934 EK160640 EK218510 EP01044 EP21153 FGH104F04 SD04406
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0002906 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 41366
Antibody Information
	polyclonal (Beall et al., 1994, Chiolo et al., 2011)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: Blm mus309 (FlyBase, 2009-)
Source for database merge of	Source for merge of: mus309 blm (Kusano et al., 2001)
Additional comments	FlyBase Curator comment: 'mus309' renamed to 'Blm' to reflect the preferred usage in the primary literature (see FBrf0155463, FBrf0216460, FBrf0193899, FBrf0207350, FBrf0107832, FBrf0135945, FBrf0180647, FBrf0200344, FBrf0179341, FBrf0191828, FBrf0202212, FBrf0193858, FBrf0209368). Moreover, symbols with 'mus' prefixes (for 'mutagen-sensitive') are deemed placeholders, to be replaced with a more meaningful symbol when available. (FlyBase, 2009-) It had previously been suggested that mus309 corresponds to Irbp (FBrf0086898). Detailed deletion mapping suggests that this was incorrect and mus309 actually corresponds to blm, which is supported by the identification of mutations in the blm coding sequence for two mus309 alleles. (Kusano et al., 2001) mus309 stated to correspond to Irbp. Subsequent information suggests that this is not the case (see Kusano et al., 2001, Science 291(5513): 2600--2602). (Beall and Rio, 1996) 'Note added in proof' in FBrf0080432 was in error; mus309, l(3)87Ae and lds are not, in fact, allelic.
Other Comments
	mus309 suppresses single strand annealing between divergent repeats (termed homologous SA), thereby preventing potentially deleterious recombination events. (Kappeler et al., 2008) mus309, in collaboration with Top3α can resolve a mobile double Holliday junction substrate through convergent branch migration (in contrast to classic resolvase activities that use a structure-specific endonuclease to cleave the Holliday junctions). (Plank et al., 2006) mus309 mutants are severely impaired in their ability to carry out repair DNA synthesis during synthesis-dependent strand annealing in double-strand break repair. (Adams et al., 2003) Designation as "DmKu70" was in error. (Kooistra et al., 1999) mus309 gene product is required for the repair of P-element induced double-strand DNA chromosome breaks, both in the presence and absence of a homologous sequence. Plasmid based P-element mobility assay demonstrates that the processing of double strand DNA breaks after P-element excision is severely affected in mus309 mutant embryos. (Beall and Rio, 1996)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_079595 RefSeq (Proteins) • NP_524319 DDBJ / EMBL / GenBank • AA695680 AAD41441 AAF54691 AAL28405 AY060857 BI637854 U92536 Entrez Gene - A searchable database of RefSeq genes. • 41366 UniProtKB/Swiss-Prot • Q9VGI8 UniProtKB/TrEMBL • Q95SC9
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Helicase, C-terminal (IPR001650) HRDC domain (IPR002121) DNA/RNA helicase, ATP-dependent, DEAH-box type, conserved site (IPR002464) DNA helicase, ATP-dependent, RecQ type (IPR004589) DNA/RNA helicase, DEAD/DEAH box type, N-terminal (IPR011545) HRDC-like (IPR010997) Helicase, superfamily 1/2, ATP-binding domain (IPR014001) RQC domain (IPR018982) P-loop containing nucleoside triphosphate hydrolase (IPR027417) Winged helix-turn-helix DNA-binding domain (IPR011991)
Linkouts
	BioGRID - A database of protein and genetic interactions • 66499 DroID - A comprehensive database of gene and protein interactions. • FBgn0002906 DRSC - Results from RNAi screens. • FBgn0002906 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0002906 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG6920-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0002906 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 41366 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 41366 modMine - Data generated by the modENCODE project. • FBgn0002906
Synonyms & Secondary IDs ( 26 )
Reported As
Symbol Synonym	blm (Sekelsky et al., 2000, Lasko, 2000, Song et al., 2007, Garcia et al., 2011) Blm (Plank et al., 2006, Chen et al., 2012, Kappeler et al., 2008, ) BLM (Johnson-Schlitz and Engels, 2006) CG6920 (Ni et al., 2008) dBLM (Song et al., 2007) DmBlm (McVey et al., 2004, Adams et al., 2003, McVey et al., 2003, Trowbridge et al., 2006, McVey et al., 2007, Traynis and McVey, 2007, Johnson-Schlitz et al., 2007, Johnson-Schlitz and Engels, 2006, Witsell et al., 2009) DmBLM (Min et al., 2004, Andersen et al., 2009, Weinert and Rio, 2007) Dmblm (Wu and Hickson, 2001, Kusano et al., 2001, Kusano et al., 2000, Kusano, 1997.3.7, Kusano et al., 1999, Engels, 1999.4.8, Hawley and Gilliland, 2006) dmblm (Brough et al., 2008) DmKu70 (Kooistra et al., 1999) Ku70 (Nicholas and Brookfield, 2002, Reis et al., 2011) mus 309 (Portin, 2003) MUS309 (Traynis and McVey, 2007) mus309 (Trowbridge et al., 2007, Trowbridge et al., 2006, Hawley and Gilliland, 2006, McVey et al., 2007, Kusano et al., 2001, LaFave et al., 2008, Wei and Rong, 2007, Doheny et al., 2008, Nicholas and Brookfield, 2002, Kappeler et al., 2008, Andersen et al., 2009, Portin, 2005, Witsell et al., 2009, Song et al., 2007, Johnson-Schlitz and Engels, 2006, Johnson-Schlitz et al., 2007, Mishra et al., 2011, Andersen et al., 2011, Garcia et al., 2011) Mus309 (Siddiqui et al., 2012) RecQ (Kusano et al., 1997, Kusano et al., 1996)
Name Synonym	bloom (Kusano et al., 1999, ) Bloom's helicase (Plank et al., 2006) Bloom helicase (Trowbridge et al., 2006, Kappeler et al., 2008) bloom syndrome helicase (Garcia et al., 2011) Bloom syndrome helicase ortholog   Drosophila Blm (McVey et al., 2006) genome stabilizer 1 (Kusano et al., 1997) gesta1 (Kusano et al., 1997, ) mutagen-sensitive 309   RecQ-like (Kusano et al., 1997, Kusano et al., 1996)
Secondary FlyBase IDs
	FBgn0015800
References ( 89 )
Generate a list of	All references relating to this gene ( 89 )
List References by type	Research paper  ( 43 ) Supplementary material  ( 4 ) Review  ( 5 ) Personal communication to FlyBase  ( 8 ) Abstract  ( 21 ) FlyBase analysis  ( 3 ) DNA/RNA sequence record  ( 1 ) Curated genome annotation  ( 2 ) Book  ( 1 ) Protein sequence record  ( 1 )
Recent research papers ( 7 )
	Chen et al., 2012, J. Biol. Chem. 287(23): 19346--19353 Essential functions of C terminus of Drosophila Topoisomerase IIIα in double holliday junction dissolution. [FBrf0219042] Siddiqui et al., 2012, Genome Biol. 13(2): R11 Genome-wide analysis of the maternal-to-zygotic transition in Drosophila primordial germ cells. [FBrf0218847] Andersen et al., 2011, PLoS Genet. 7(10): e1002315 Three Structure-Selective Endonucleases Are Essential in the Absence of BLM Helicase in Drosophila. [FBrf0216460] Chiolo et al., 2011, Cell 144(5): 732--744 Double-Strand Breaks in Heterochromatin Move Outside of a Dynamic HP1a Domain to Complete Recombinational Repair. [FBrf0213146] Garcia et al., 2011, Genome Biol. 12(12): R121 Loss of the bloom syndrome helicase increases DNA ligase 4-independent genome rearrangements and tumorigenesis in aging Drosophila. [FBrf0218165] Mishra et al., 2011, Mutat. Res. 722(1): 44--51 Tracing the tracks of genotoxicity by trivalent and hexavalent chromium in Drosophila melanogaster. [FBrf0213784] Reis et al., 2011, PLoS ONE 6(3): e17512 Drosophila Genes That Affect Meiosis Duration Are among the Meiosis Related Genes That Are More Often Found Duplicated. [FBrf0213266] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...