Gene Dmel\Myb

13F-13F

(determined by in situ hybridisation)

(Katzen et al., 1985)

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Gene Model & Products

Please see the GBrowse view of Dmel\Myb for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Comments on Gene Model

Gene model reviewed during 5.50

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Low-frequency RNA-Seq exon junction(s) not annotated.

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

Myb-RA

FBtr0074169

NM_078615

2788

657

Myb-RB

FBtr0074170

NM_206734

2730

657

Myb-RC

FBtr0074172

NM_206732

2806

657

Myb-RD

FBtr0074171

NM_206733

2863

657

Myb-RE

FBtr0074173

NM_206731

2901

657

Additional Transcript Data & Comments

Reported size (kB)

3.2 (northern blot)

2.814 (longest cDNA)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

Myb-PA

FBpp0073961

74.0

657

9.20

NP_511170

AAF48529

Myb-PB

FBpp0089402

74.0

657

9.20

NP_996457

AAS65355

Myb-PC

FBpp0089404

74.0

657

9.20

NP_996455

AAS65356

Myb-PD

FBpp0089403

74.0

657

9.20

NP_996456

AAS65357

Myb-PE

FBpp0089405

74.0

657

9.20

NP_996454

AAS65358

Additional Polypeptide Data & Comments

Reported size (kDa)

657 (aa); 74 (kD predicted)

Comments

External Data

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

SANT/Myb domain (IPR001005)

Homeodomain-like (IPR009057)

C-myb, C-terminal (IPR015395)

Myb-like domain (IPR017877)

Myb domain (IPR017930)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

P04197

UniProtKB/TrEMBL

Q53YG3

Mapped Features

Mapped Features have been reorganized, please see this article for details.

Additional mapped features and mutations can be found on GBrowse or related reports.

Type

Symbol & Location

Additional Notes

References

External Data

Crossreferences

Expression Data

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment:maternally deposited

embryonic stage 4 -- 6

organism

embryonic stage 7 -- 8

organism | ubiquitous

embryonic stage 9 -- 10

organism | ubiquitous

embryonic stage 11 -- 12

organism | ubiquitous

embryonic stage 13 -- 16

organism | ubiquitous

radioisotope in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage | early

embryonic stage | late

presumptive embryonic/larval central nervous system

third instar larval stage

ovary

testis

imaginal disc

brain

ventral nerve cord

pupal stage | early

adult brain

imaginal disc

thoracico-abdominal ganglion

pupal stage

nervous system

pupal stage -- adult stage

ovary

testis

adult stage

adult brain

thoracico-abdominal ganglion

adult stage & oogenesis

oocyte

nurse cell

RT-PCR

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage | female

ovary

(Beall et al., 2004)

Additional Descriptive Data

The 3.2 kb Myb transcript is detected throughout development. Myb transcript is detected mainly in proliferating tissues, though expression is seen at lower levels in some post-mitotic tissues. Expression is uniform and high in preblastoderm embryos, and continues to be high during cellular blastoderm formation and germ band extension. Message is less uniform in contracted germ band embryos, with no expression seen in the amnioserosa, and low expression in the salivary glands. Later in embryogenesis, Myb transcript is high only in the CNS. In climbing third instar larvae, tissues contributing to the adult express Myb at high levels: imaginal disc, brain, ventral ganglion, testes, ovaries, and nests of imaginal cells in the gut. Expression in these tissues continues in the early pupa, with additional expression detected in histoblasts and epithelial cells of the developing imaginal gut. Myb levels decline in everted imaginal tissues. Ovary, testis, and neural tissue continue to express Myb transcript through pupal development. Myb is detected at low levels in the brain and thoracic ganglia of adults, and expression is high in adult testes and ovaries.

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage | female

follicle cell

• nucleus

(Beall et al., 2004)

western blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- adult stage

organism

(Beall et al., 2004)

Additional Descriptive Data

Marker for

Subcellular Localization (GO Cellular Component)

CV term

(Beall et al., 2002, Lewis et al., 2004, Korenjak et al., 2004)

References

Myb complex

inferred from direct assay

nuclear euchromatin

inferred from direct assay

(Manak et al., 2007, Scaria et al., 2008)

nucleus

inferred from direct assay

(The modENCODE Consortium, 2010, Graveley et al., 2011)

polytene chromosome

inferred from direct assay

(Beall et al., 2007)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

Reference

See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.

FlyAtlas Anatomy Microarray

FlyAtlas Anatomical Expression Data

(FlyAtlas-RNA.adult)

(FlyAtlas-RNA.larva)

	Styles
	Linear
	Logarithmic
	Heatmap
	Back-to-back
	Scales
	gene max expression
	Moderate expression bin max
	High level expression bin max
	Very high expression bin max

Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult tissues/organs expressed at moderate levels. Expression at high levels in the following post-embryonic organs or tissues: larval central nervous system. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male reproductive system, larval/adult carcass.
[download data (TSV)]

Guide to FlyAtlas expression level colors
	No expression (0 - 9.999)
	Low expression (10 - 99.999)
	Moderate expression (100 - 499.999)
	High level expression (500 - 999.999)
	Very high expression (>999.999)

Linear, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		576.625
Larval Midgut		120.7
Larval Hindgut		167.9
Larval Malpighian Tubules		242.9
Larval Fat Body		163.9
Larval Salivary Gland		155
Larval Trachea		252.825
Larval Carcass		175.375
Adult Head		197.5
Adult Eye		193.025
Adult Brain		276
Adult Thoracic-Abdominal Ganglion		246.8
Adult Crop		240.3
Adult Midgut		158.7
Adult Hindgut		163.5
Adult Malpighian Tubules		148.1
Adult Fat Body		112.8
Adult Salivary Gland		172.4
Adult Heart		104.85
Adult VirginFemale Spermatheca		99.5
Adult InseminatedFemale Spermatheca		109.5
Adult Ovary		340.2
Adult Testis		235.9
Adult Male Accessory Gland		178
Adult Carcass		117.8
Expression Level Scale		None Low Moderate High

Linear, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		(576.625)
Larval Midgut		120.7
Larval Hindgut		167.9
Larval Malpighian Tubules		242.9
Larval Fat Body		163.9
Larval Salivary Gland		155
Larval Trachea		252.825
Larval Carcass		175.375
Adult Head		197.5
Adult Eye		193.025
Adult Brain		276
Adult Thoracic-Abdominal Ganglion		246.8
Adult Crop		240.3
Adult Midgut		158.7
Adult Hindgut		163.5
Adult Malpighian Tubules		148.1
Adult Fat Body		112.8
Adult Salivary Gland		172.4
Adult Heart		104.85
Adult VirginFemale Spermatheca		99.5
Adult InseminatedFemale Spermatheca		109.5
Adult Ovary		340.2
Adult Testis		235.9
Adult Male Accessory Gland		178
Adult Carcass		117.8
Expression Level Scale		None Low Moderate High

Linear, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		576.625
Larval Midgut		120.7
Larval Hindgut		167.9
Larval Malpighian Tubules		242.9
Larval Fat Body		163.9
Larval Salivary Gland		155
Larval Trachea		252.825
Larval Carcass		175.375
Adult Head		197.5
Adult Eye		193.025
Adult Brain		276
Adult Thoracic-Abdominal Ganglion		246.8
Adult Crop		240.3
Adult Midgut		158.7
Adult Hindgut		163.5
Adult Malpighian Tubules		148.1
Adult Fat Body		112.8
Adult Salivary Gland		172.4
Adult Heart		104.85
Adult VirginFemale Spermatheca		99.5
Adult InseminatedFemale Spermatheca		109.5
Adult Ovary		340.2
Adult Testis		235.9
Adult Male Accessory Gland		178
Adult Carcass		117.8
Expression Level Scale		None Low Moderate High Very high

Linear, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		576.625
Larval Midgut		120.7
Larval Hindgut		167.9
Larval Malpighian Tubules		242.9
Larval Fat Body		163.9
Larval Salivary Gland		155
Larval Trachea		252.825
Larval Carcass		175.375
Adult Head		197.5
Adult Eye		193.025
Adult Brain		276
Adult Thoracic-Abdominal Ganglion		246.8
Adult Crop		240.3
Adult Midgut		158.7
Adult Hindgut		163.5
Adult Malpighian Tubules		148.1
Adult Fat Body		112.8
Adult Salivary Gland		172.4
Adult Heart		104.85
Adult VirginFemale Spermatheca		99.5
Adult InseminatedFemale Spermatheca		109.5
Adult Ovary		340.2
Adult Testis		235.9
Adult Male Accessory Gland		178
Adult Carcass		117.8
Expression Level Scale		Very high

log, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		576.625
Larval Midgut		120.7
Larval Hindgut		167.9
Larval Malpighian Tubules		242.9
Larval Fat Body		163.9
Larval Salivary Gland		155
Larval Trachea		252.825
Larval Carcass		175.375
Adult Head		197.5
Adult Eye		193.025
Adult Brain		276
Adult Thoracic-Abdominal Ganglion		246.8
Adult Crop		240.3
Adult Midgut		158.7
Adult Hindgut		163.5
Adult Malpighian Tubules		148.1
Adult Fat Body		112.8
Adult Salivary Gland		172.4
Adult Heart		104.85
Adult VirginFemale Spermatheca		99.5
Adult InseminatedFemale Spermatheca		109.5
Adult Ovary		340.2
Adult Testis		235.9
Adult Male Accessory Gland		178
Adult Carcass		117.8
Expression Level Scale		None Low Moderate High Very high

log, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		576.625
Larval Midgut		120.7
Larval Hindgut		167.9
Larval Malpighian Tubules		242.9
Larval Fat Body		163.9
Larval Salivary Gland		155
Larval Trachea		252.825
Larval Carcass		175.375
Adult Head		197.5
Adult Eye		193.025
Adult Brain		276
Adult Thoracic-Abdominal Ganglion		246.8
Adult Crop		240.3
Adult Midgut		158.7
Adult Hindgut		163.5
Adult Malpighian Tubules		148.1
Adult Fat Body		112.8
Adult Salivary Gland		172.4
Adult Heart		104.85
Adult VirginFemale Spermatheca		99.5
Adult InseminatedFemale Spermatheca		109.5
Adult Ovary		340.2
Adult Testis		235.9
Adult Male Accessory Gland		178
Adult Carcass		117.8
Expression Level Scale		None Low Moderate High

log, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		576.625
Larval Midgut		120.7
Larval Hindgut		167.9
Larval Malpighian Tubules		242.9
Larval Fat Body		163.9
Larval Salivary Gland		155
Larval Trachea		252.825
Larval Carcass		175.375
Adult Head		197.5
Adult Eye		193.025
Adult Brain		276
Adult Thoracic-Abdominal Ganglion		246.8
Adult Crop		240.3
Adult Midgut		158.7
Adult Hindgut		163.5
Adult Malpighian Tubules		148.1
Adult Fat Body		112.8
Adult Salivary Gland		172.4
Adult Heart		104.85
Adult VirginFemale Spermatheca		99.5
Adult InseminatedFemale Spermatheca		109.5
Adult Ovary		340.2
Adult Testis		235.9
Adult Male Accessory Gland		178
Adult Carcass		117.8
Expression Level Scale		None Low Moderate High Very high

log, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		576.625
Larval Midgut		120.7
Larval Hindgut		167.9
Larval Malpighian Tubules		242.9
Larval Fat Body		163.9
Larval Salivary Gland		155
Larval Trachea		252.825
Larval Carcass		175.375
Adult Head		197.5
Adult Eye		193.025
Adult Brain		276
Adult Thoracic-Abdominal Ganglion		246.8
Adult Crop		240.3
Adult Midgut		158.7
Adult Hindgut		163.5
Adult Malpighian Tubules		148.1
Adult Fat Body		112.8
Adult Salivary Gland		172.4
Adult Heart		104.85
Adult VirginFemale Spermatheca		99.5
Adult InseminatedFemale Spermatheca		109.5
Adult Ovary		340.2
Adult Testis		235.9
Adult Male Accessory Gland		178
Adult Carcass		117.8
Expression Level Scale		None Low Moderate High Very high

Heatmap
Tissue		Expression Level
Larval Central Nervous System
Larval Midgut
Larval Hindgut
Larval Malpighian Tubules
Larval Fat Body
Larval Salivary Gland
Larval Trachea
Larval Carcass
Adult Head
Adult Eye
Adult Brain
Adult Thoracic-Abdominal Ganglion
Adult Crop
Adult Midgut
Adult Hindgut
Adult Malpighian Tubules
Adult Fat Body
Adult Salivary Gland
Adult Heart
Adult VirginFemale Spermatheca
Adult InseminatedFemale Spermatheca
Adult Ovary
Adult Testis
Adult Male Accessory Gland
Adult Carcass

FlyAtlas Organ/Tissue Expression, larval vs. adult
Larval Expression Level	Tissue	Adult Expression Level
NA	Head	197.5
NA	Eye	193.025
NA	Brain	276
576.625	Central Nervous System	NA
NA	Thoracic-Abdominal Ganglion	246.8
NA	Crop	240.3
120.7	Midgut	158.7
167.9	Hindgut	163.5
242.9	Malpighian Tubules	148.1
163.9	Fat Body	112.8
155	Salivary Gland	172.4
NA	Heart	104.85
252.825	Trachea	NA
NA	VirginFemale Spermatheca	99.5
NA	InseminatedFemale Spermatheca	109.5
NA	Ovary	340.2
NA	Testis	235.9
NA	Male Accessory Gland	178
175.375	Carcass	117.8

FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)

modENCODE Anatomy RNA-Seq

modENCODE Tissue Expression Data

(modENCODE_mRNA-Seq_tissues)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		81
central nervous system, larvae L3		114
central nervous system, pupae P8		46
head, virgin 1-day female		26
head, virgin 4-day female		35
head, virgin 20-day female		31
head, mated 1-day female		29
head, mated 4-day female		32
head, mated 20-day female		26
head, mated 1-day male		42
head, mated 4-day male		46
head, mated 20-day male		60
salivary gland, larvae L3 wandering		5
salivary gland, white prepupae		17
digestive system, larvae L3 wandering		45
digestive system, 1-day adult		20
digestive system, 4-day adult		24
digestive system, 20-day adult		21
fat body, larvae L3 wandering		10
fat body, white prepupae		36
fat body, pupae P8		54
carcass, larvae L3 wandering		78
carcass, 1-day adult		17
carcass, 4-day adult		21
carcass, 20-day adult		24
ovary, virgin 4-day female		108
ovary, mated 4-day female		105
testis, mated 4-day male		68
accessory gland, mated 4-day male		24
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		(81)
central nervous system, larvae L3		(114)
central nervous system, pupae P8		(46)
head, virgin 1-day female		26
head, virgin 4-day female		(35)
head, virgin 20-day female		(31)
head, mated 1-day female		(29)
head, mated 4-day female		(32)
head, mated 20-day female		26
head, mated 1-day male		(42)
head, mated 4-day male		(46)
head, mated 20-day male		(60)
salivary gland, larvae L3 wandering		5
salivary gland, white prepupae		17
digestive system, larvae L3 wandering		(45)
digestive system, 1-day adult		20
digestive system, 4-day adult		24
digestive system, 20-day adult		21
fat body, larvae L3 wandering		10
fat body, white prepupae		(36)
fat body, pupae P8		(54)
carcass, larvae L3 wandering		(78)
carcass, 1-day adult		17
carcass, 4-day adult		21
carcass, 20-day adult		24
ovary, virgin 4-day female		(108)
ovary, mated 4-day female		(105)
testis, mated 4-day male		(68)
accessory gland, mated 4-day male		24
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		81
central nervous system, larvae L3		(114)
central nervous system, pupae P8		46
head, virgin 1-day female		26
head, virgin 4-day female		35
head, virgin 20-day female		31
head, mated 1-day female		29
head, mated 4-day female		32
head, mated 20-day female		26
head, mated 1-day male		42
head, mated 4-day male		46
head, mated 20-day male		60
salivary gland, larvae L3 wandering		5
salivary gland, white prepupae		17
digestive system, larvae L3 wandering		45
digestive system, 1-day adult		20
digestive system, 4-day adult		24
digestive system, 20-day adult		21
fat body, larvae L3 wandering		10
fat body, white prepupae		36
fat body, pupae P8		54
carcass, larvae L3 wandering		78
carcass, 1-day adult		17
carcass, 4-day adult		21
carcass, 20-day adult		24
ovary, virgin 4-day female		(108)
ovary, mated 4-day female		105
testis, mated 4-day male		68
accessory gland, mated 4-day male		24
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		81
central nervous system, larvae L3		114
central nervous system, pupae P8		46
head, virgin 1-day female		26
head, virgin 4-day female		35
head, virgin 20-day female		31
head, mated 1-day female		29
head, mated 4-day female		32
head, mated 20-day female		26
head, mated 1-day male		42
head, mated 4-day male		46
head, mated 20-day male		60
salivary gland, larvae L3 wandering		5
salivary gland, white prepupae		17
digestive system, larvae L3 wandering		45
digestive system, 1-day adult		20
digestive system, 4-day adult		24
digestive system, 20-day adult		21
fat body, larvae L3 wandering		10
fat body, white prepupae		36
fat body, pupae P8		54
carcass, larvae L3 wandering		78
carcass, 1-day adult		17
carcass, 4-day adult		21
carcass, 20-day adult		24
ovary, virgin 4-day female		108
ovary, mated 4-day female		105
testis, mated 4-day male		68
accessory gland, mated 4-day male		24
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		81
central nervous system, larvae L3		114
central nervous system, pupae P8		46
head, virgin 1-day female		26
head, virgin 4-day female		35
head, virgin 20-day female		31
head, mated 1-day female		29
head, mated 4-day female		32
head, mated 20-day female		26
head, mated 1-day male		42
head, mated 4-day male		46
head, mated 20-day male		60
salivary gland, larvae L3 wandering		5
salivary gland, white prepupae		17
digestive system, larvae L3 wandering		45
digestive system, 1-day adult		20
digestive system, 4-day adult		24
digestive system, 20-day adult		21
fat body, larvae L3 wandering		10
fat body, white prepupae		36
fat body, pupae P8		54
carcass, larvae L3 wandering		78
carcass, 1-day adult		17
carcass, 4-day adult		21
carcass, 20-day adult		24
ovary, virgin 4-day female		108
ovary, mated 4-day female		105
testis, mated 4-day male		68
accessory gland, mated 4-day male		24
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		(81)
central nervous system, larvae L3		(114)
central nervous system, pupae P8		(46)
head, virgin 1-day female		26
head, virgin 4-day female		(35)
head, virgin 20-day female		31
head, mated 1-day female		29
head, mated 4-day female		32
head, mated 20-day female		26
head, mated 1-day male		(42)
head, mated 4-day male		(46)
head, mated 20-day male		(60)
salivary gland, larvae L3 wandering		5
salivary gland, white prepupae		17
digestive system, larvae L3 wandering		(45)
digestive system, 1-day adult		20
digestive system, 4-day adult		24
digestive system, 20-day adult		21
fat body, larvae L3 wandering		10
fat body, white prepupae		(36)
fat body, pupae P8		(54)
carcass, larvae L3 wandering		(78)
carcass, 1-day adult		17
carcass, 4-day adult		21
carcass, 20-day adult		24
ovary, virgin 4-day female		(108)
ovary, mated 4-day female		(105)
testis, mated 4-day male		(68)
accessory gland, mated 4-day male		24
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		81
central nervous system, larvae L3		114
central nervous system, pupae P8		46
head, virgin 1-day female		26
head, virgin 4-day female		35
head, virgin 20-day female		31
head, mated 1-day female		29
head, mated 4-day female		32
head, mated 20-day female		26
head, mated 1-day male		42
head, mated 4-day male		46
head, mated 20-day male		60
salivary gland, larvae L3 wandering		5
salivary gland, white prepupae		17
digestive system, larvae L3 wandering		45
digestive system, 1-day adult		20
digestive system, 4-day adult		24
digestive system, 20-day adult		21
fat body, larvae L3 wandering		10
fat body, white prepupae		36
fat body, pupae P8		54
carcass, larvae L3 wandering		78
carcass, 1-day adult		17
carcass, 4-day adult		21
carcass, 20-day adult		24
ovary, virgin 4-day female		108
ovary, mated 4-day female		105
testis, mated 4-day male		68
accessory gland, mated 4-day male		24
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		81
central nervous system, larvae L3		114
central nervous system, pupae P8		46
head, virgin 1-day female		26
head, virgin 4-day female		35
head, virgin 20-day female		31
head, mated 1-day female		29
head, mated 4-day female		32
head, mated 20-day female		26
head, mated 1-day male		42
head, mated 4-day male		46
head, mated 20-day male		60
salivary gland, larvae L3 wandering		5
salivary gland, white prepupae		17
digestive system, larvae L3 wandering		45
digestive system, 1-day adult		20
digestive system, 4-day adult		24
digestive system, 20-day adult		21
fat body, larvae L3 wandering		10
fat body, white prepupae		36
fat body, pupae P8		54
carcass, larvae L3 wandering		78
carcass, 1-day adult		17
carcass, 4-day adult		21
carcass, 20-day adult		24
ovary, virgin 4-day female		108
ovary, mated 4-day female		105
testis, mated 4-day male		68
accessory gland, mated 4-day male		24
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Tissue		Expression Level
imaginal disc, larvae L3 wandering
central nervous system, larvae L3
central nervous system, pupae P8
head, virgin 1-day female
head, virgin 4-day female
head, virgin 20-day female
head, mated 1-day female
head, mated 4-day female
head, mated 20-day female
head, mated 1-day male
head, mated 4-day male
head, mated 20-day male
salivary gland, larvae L3 wandering
salivary gland, white prepupae
digestive system, larvae L3 wandering
digestive system, 1-day adult
digestive system, 4-day adult
digestive system, 20-day adult
fat body, larvae L3 wandering
fat body, white prepupae
fat body, pupae P8
carcass, larvae L3 wandering
carcass, 1-day adult
carcass, 4-day adult
carcass, 20-day adult
ovary, virgin 4-day female
ovary, mated 4-day female
testis, mated 4-day male
accessory gland, mated 4-day male

modENCODE Development RNA-Seq

modENCODE Temporal Expression Data

(modENCODE_mRNA-Seq_U)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-18 hour embryonic stages, during early pupal stages.
[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		71
embryo 02-04hr		83
embryo 04-06hr		102
embryo 06-08hr		114
embryo 08-10hr		99
embryo 10-12hr		68
embryo 12-14hr		60
embryo 14-16hr		47
embryo 16-18hr		46
embryo 18-20hr		34
embryo 20-22hr		50
embryo 22-24hr		46
larva L1		24
larva L2		17
larva L3 12hr old		14
larva L3 puffstage 1-2		22
larva L3 puffstage 3-6		33
larva L3 puffstage 7-9		42
white prepupae new		42
white prepupae 12hr		48
white prepupae 24hr		54
pupae 2d postWPP		34
pupae 3d postWPP		27
pupae 4d postWPP		19
adult male 01day		29
adult male 05day		34
adult male 30day		34
adult female 01day		34
adult female 05day		51
adult female 30day		51
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		(71)
embryo 02-04hr		(83)
embryo 04-06hr		(102)
embryo 06-08hr		(114)
embryo 08-10hr		(99)
embryo 10-12hr		(68)
embryo 12-14hr		(60)
embryo 14-16hr		(47)
embryo 16-18hr		(46)
embryo 18-20hr		(34)
embryo 20-22hr		(50)
embryo 22-24hr		(46)
larva L1		24
larva L2		17
larva L3 12hr old		14
larva L3 puffstage 1-2		22
larva L3 puffstage 3-6		(33)
larva L3 puffstage 7-9		(42)
white prepupae new		(42)
white prepupae 12hr		(48)
white prepupae 24hr		(54)
pupae 2d postWPP		(34)
pupae 3d postWPP		(27)
pupae 4d postWPP		19
adult male 01day		(29)
adult male 05day		(34)
adult male 30day		(34)
adult female 01day		(34)
adult female 05day		(51)
adult female 30day		(51)
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		71
embryo 02-04hr		83
embryo 04-06hr		102
embryo 06-08hr		(114)
embryo 08-10hr		99
embryo 10-12hr		68
embryo 12-14hr		60
embryo 14-16hr		47
embryo 16-18hr		46
embryo 18-20hr		34
embryo 20-22hr		50
embryo 22-24hr		46
larva L1		24
larva L2		17
larva L3 12hr old		14
larva L3 puffstage 1-2		22
larva L3 puffstage 3-6		33
larva L3 puffstage 7-9		42
white prepupae new		42
white prepupae 12hr		48
white prepupae 24hr		54
pupae 2d postWPP		34
pupae 3d postWPP		27
pupae 4d postWPP		19
adult male 01day		29
adult male 05day		34
adult male 30day		34
adult female 01day		34
adult female 05day		51
adult female 30day		51
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		71
embryo 02-04hr		83
embryo 04-06hr		102
embryo 06-08hr		114
embryo 08-10hr		99
embryo 10-12hr		68
embryo 12-14hr		60
embryo 14-16hr		47
embryo 16-18hr		46
embryo 18-20hr		34
embryo 20-22hr		50
embryo 22-24hr		46
larva L1		24
larva L2		17
larva L3 12hr old		14
larva L3 puffstage 1-2		22
larva L3 puffstage 3-6		33
larva L3 puffstage 7-9		42
white prepupae new		42
white prepupae 12hr		48
white prepupae 24hr		54
pupae 2d postWPP		34
pupae 3d postWPP		27
pupae 4d postWPP		19
adult male 01day		29
adult male 05day		34
adult male 30day		34
adult female 01day		34
adult female 05day		51
adult female 30day		51
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		71
embryo 02-04hr		83
embryo 04-06hr		102
embryo 06-08hr		114
embryo 08-10hr		99
embryo 10-12hr		68
embryo 12-14hr		60
embryo 14-16hr		47
embryo 16-18hr		46
embryo 18-20hr		34
embryo 20-22hr		50
embryo 22-24hr		46
larva L1		24
larva L2		17
larva L3 12hr old		14
larva L3 puffstage 1-2		22
larva L3 puffstage 3-6		33
larva L3 puffstage 7-9		42
white prepupae new		42
white prepupae 12hr		48
white prepupae 24hr		54
pupae 2d postWPP		34
pupae 3d postWPP		27
pupae 4d postWPP		19
adult male 01day		29
adult male 05day		34
adult male 30day		34
adult female 01day		34
adult female 05day		51
adult female 30day		51
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		(71)
embryo 02-04hr		(83)
embryo 04-06hr		(102)
embryo 06-08hr		(114)
embryo 08-10hr		(99)
embryo 10-12hr		(68)
embryo 12-14hr		(60)
embryo 14-16hr		(47)
embryo 16-18hr		(46)
embryo 18-20hr		(34)
embryo 20-22hr		(50)
embryo 22-24hr		(46)
larva L1		24
larva L2		17
larva L3 12hr old		14
larva L3 puffstage 1-2		22
larva L3 puffstage 3-6		(33)
larva L3 puffstage 7-9		(42)
white prepupae new		(42)
white prepupae 12hr		(48)
white prepupae 24hr		(54)
pupae 2d postWPP		(34)
pupae 3d postWPP		27
pupae 4d postWPP		19
adult male 01day		29
adult male 05day		(34)
adult male 30day		(34)
adult female 01day		(34)
adult female 05day		(51)
adult female 30day		(51)
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		71
embryo 02-04hr		83
embryo 04-06hr		102
embryo 06-08hr		114
embryo 08-10hr		99
embryo 10-12hr		68
embryo 12-14hr		60
embryo 14-16hr		47
embryo 16-18hr		46
embryo 18-20hr		34
embryo 20-22hr		50
embryo 22-24hr		46
larva L1		24
larva L2		17
larva L3 12hr old		14
larva L3 puffstage 1-2		22
larva L3 puffstage 3-6		33
larva L3 puffstage 7-9		42
white prepupae new		42
white prepupae 12hr		48
white prepupae 24hr		54
pupae 2d postWPP		34
pupae 3d postWPP		27
pupae 4d postWPP		19
adult male 01day		29
adult male 05day		34
adult male 30day		34
adult female 01day		34
adult female 05day		51
adult female 30day		51
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		71
embryo 02-04hr		83
embryo 04-06hr		102
embryo 06-08hr		114
embryo 08-10hr		99
embryo 10-12hr		68
embryo 12-14hr		60
embryo 14-16hr		47
embryo 16-18hr		46
embryo 18-20hr		34
embryo 20-22hr		50
embryo 22-24hr		46
larva L1		24
larva L2		17
larva L3 12hr old		14
larva L3 puffstage 1-2		22
larva L3 puffstage 3-6		33
larva L3 puffstage 7-9		42
white prepupae new		42
white prepupae 12hr		48
white prepupae 24hr		54
pupae 2d postWPP		34
pupae 3d postWPP		27
pupae 4d postWPP		19
adult male 01day		29
adult male 05day		34
adult male 30day		34
adult female 01day		34
adult female 05day		51
adult female 30day		51
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Developmental Stage		Expression Level
embryo 00-02hr
embryo 02-04hr
embryo 04-06hr
embryo 06-08hr
embryo 08-10hr
embryo 10-12hr
embryo 12-14hr
embryo 14-16hr
embryo 16-18hr
embryo 18-20hr
embryo 20-22hr
embryo 22-24hr
larva L1
larva L2
larva L3 12hr old
larva L3 puffstage 1-2
larva L3 puffstage 3-6
larva L3 puffstage 7-9
white prepupae new
white prepupae 12hr
white prepupae 24hr
pupae 2d postWPP
pupae 3d postWPP
pupae 4d postWPP
adult male 01day
adult male 05day
adult male 30day
adult female 01day
adult female 05day
adult female 30day

modENCODE Temporal Expression Data (Graveley et al., 2011)

modENCODE Cell Lines RNA-Seq

modENCODE Cell Line Expression Data

(modENCODE_mRNA-Seq_cell.A)

(modENCODE_mRNA-Seq_cell.B)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		126
Schneider line 2 Sg4		100
embryonic 1182-4H		173
embryonic GM2		89
embryonic Kc167		71
embryonic S1		237
embryonic S3		89
leg disc CME L1		154
wing disc CME-W2		81
wing disc ML-DmD8		102
wing disc ML-DmD9		142
wing disc ML-DmD16-c3		128
wing disc ML-DmD21		111
wing disc ML-DmD32		170
haltere disc ML-DmD17-c3		76
eye-antennal disc ML-DmD11		145
antennal disc ML-DmD20-c5		69
mixed discs ML-DmD4-c1		101
CNS ML-DmBG1-c1		106
CNS ML-DmBG2-c2		94
tumorous blood cells mbn2		61
ovary fGS/OSS		172
ovary OSC		109
ovary OSS		75
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		(126)
Schneider line 2 Sg4		(100)
embryonic 1182-4H		(173)
embryonic GM2		(89)
embryonic Kc167		(71)
embryonic S1		(237)
embryonic S3		(89)
leg disc CME L1		(154)
wing disc CME-W2		(81)
wing disc ML-DmD8		(102)
wing disc ML-DmD9		(142)
wing disc ML-DmD16-c3		(128)
wing disc ML-DmD21		(111)
wing disc ML-DmD32		(170)
haltere disc ML-DmD17-c3		(76)
eye-antennal disc ML-DmD11		(145)
antennal disc ML-DmD20-c5		(69)
mixed discs ML-DmD4-c1		(101)
CNS ML-DmBG1-c1		(106)
CNS ML-DmBG2-c2		(94)
tumorous blood cells mbn2		(61)
ovary fGS/OSS		(172)
ovary OSC		(109)
ovary OSS		(75)
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		(126)
Schneider line 2 Sg4		100
embryonic 1182-4H		(173)
embryonic GM2		89
embryonic Kc167		71
embryonic S1		(237)
embryonic S3		89
leg disc CME L1		(154)
wing disc CME-W2		81
wing disc ML-DmD8		102
wing disc ML-DmD9		(142)
wing disc ML-DmD16-c3		(128)
wing disc ML-DmD21		(111)
wing disc ML-DmD32		(170)
haltere disc ML-DmD17-c3		76
eye-antennal disc ML-DmD11		(145)
antennal disc ML-DmD20-c5		69
mixed discs ML-DmD4-c1		101
CNS ML-DmBG1-c1		106
CNS ML-DmBG2-c2		94
tumorous blood cells mbn2		61
ovary fGS/OSS		(172)
ovary OSC		(109)
ovary OSS		75
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		126
Schneider line 2 Sg4		100
embryonic 1182-4H		173
embryonic GM2		89
embryonic Kc167		71
embryonic S1		237
embryonic S3		89
leg disc CME L1		154
wing disc CME-W2		81
wing disc ML-DmD8		102
wing disc ML-DmD9		142
wing disc ML-DmD16-c3		128
wing disc ML-DmD21		111
wing disc ML-DmD32		170
haltere disc ML-DmD17-c3		76
eye-antennal disc ML-DmD11		145
antennal disc ML-DmD20-c5		69
mixed discs ML-DmD4-c1		101
CNS ML-DmBG1-c1		106
CNS ML-DmBG2-c2		94
tumorous blood cells mbn2		61
ovary fGS/OSS		172
ovary OSC		109
ovary OSS		75
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		126
Schneider line 2 Sg4		100
embryonic 1182-4H		173
embryonic GM2		89
embryonic Kc167		71
embryonic S1		237
embryonic S3		89
leg disc CME L1		154
wing disc CME-W2		81
wing disc ML-DmD8		102
wing disc ML-DmD9		142
wing disc ML-DmD16-c3		128
wing disc ML-DmD21		111
wing disc ML-DmD32		170
haltere disc ML-DmD17-c3		76
eye-antennal disc ML-DmD11		145
antennal disc ML-DmD20-c5		69
mixed discs ML-DmD4-c1		101
CNS ML-DmBG1-c1		106
CNS ML-DmBG2-c2		94
tumorous blood cells mbn2		61
ovary fGS/OSS		172
ovary OSC		109
ovary OSS		75
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		(126)
Schneider line 2 Sg4		(100)
embryonic 1182-4H		(173)
embryonic GM2		(89)
embryonic Kc167		(71)
embryonic S1		(237)
embryonic S3		(89)
leg disc CME L1		(154)
wing disc CME-W2		(81)
wing disc ML-DmD8		(102)
wing disc ML-DmD9		(142)
wing disc ML-DmD16-c3		(128)
wing disc ML-DmD21		(111)
wing disc ML-DmD32		(170)
haltere disc ML-DmD17-c3		(76)
eye-antennal disc ML-DmD11		(145)
antennal disc ML-DmD20-c5		(69)
mixed discs ML-DmD4-c1		(101)
CNS ML-DmBG1-c1		(106)
CNS ML-DmBG2-c2		(94)
tumorous blood cells mbn2		(61)
ovary fGS/OSS		(172)
ovary OSC		(109)
ovary OSS		(75)
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		126
Schneider line 2 Sg4		100
embryonic 1182-4H		(173)
embryonic GM2		89
embryonic Kc167		71
embryonic S1		(237)
embryonic S3		89
leg disc CME L1		(154)
wing disc CME-W2		81
wing disc ML-DmD8		102
wing disc ML-DmD9		142
wing disc ML-DmD16-c3		128
wing disc ML-DmD21		111
wing disc ML-DmD32		(170)
haltere disc ML-DmD17-c3		76
eye-antennal disc ML-DmD11		(145)
antennal disc ML-DmD20-c5		69
mixed discs ML-DmD4-c1		101
CNS ML-DmBG1-c1		106
CNS ML-DmBG2-c2		94
tumorous blood cells mbn2		61
ovary fGS/OSS		(172)
ovary OSC		109
ovary OSS		75
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		126
Schneider line 2 Sg4		100
embryonic 1182-4H		173
embryonic GM2		89
embryonic Kc167		71
embryonic S1		237
embryonic S3		89
leg disc CME L1		154
wing disc CME-W2		81
wing disc ML-DmD8		102
wing disc ML-DmD9		142
wing disc ML-DmD16-c3		128
wing disc ML-DmD21		111
wing disc ML-DmD32		170
haltere disc ML-DmD17-c3		76
eye-antennal disc ML-DmD11		145
antennal disc ML-DmD20-c5		69
mixed discs ML-DmD4-c1		101
CNS ML-DmBG1-c1		106
CNS ML-DmBG2-c2		94
tumorous blood cells mbn2		61
ovary fGS/OSS		172
ovary OSC		109
ovary OSS		75
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Cell Line		Expression Level
Schneider line 2 S2R+
Schneider line 2 Sg4
embryonic 1182-4H
embryonic GM2
embryonic Kc167
embryonic S1
embryonic S3
leg disc CME L1
wing disc CME-W2
wing disc ML-DmD8
wing disc ML-DmD9
wing disc ML-DmD16-c3
wing disc ML-DmD21
wing disc ML-DmD32
haltere disc ML-DmD17-c3
eye-antennal disc ML-DmD11
antennal disc ML-DmD20-c5
mixed discs ML-DmD4-c1
CNS ML-DmBG1-c1
CNS ML-DmBG2-c2
tumorous blood cells mbn2
ovary fGS/OSS
ovary OSC
ovary OSS

modENCODE Treatments RNA-Seq

modENCODE Treatment Expression Data

(modENCODE_mRNA-Seq_treatments)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		36
cold shock, 4-day adult		29
heat shock, 4-day adult		40
Cadmium 50 mM 6 hrs, larvae L3		21
Cadmium 50 mM 12 hrs, larvae L3		31
Cadmium 50 mM 48 hrs, 4-day adult		41
Cadmium 100 mM 48 hrs, 4-day adult		77
Copper 0.5 mM 12 hrs, larvae L3		21
Copper 15 mM 48 hrs, 4-day adult		50
Zinc 5 mM 12 hrs, larvae L3		19
Zinc 4.5 mM 48 hrs, 4-day adult		44
Ethanol 2.5% 3 hrs, larvae L3		67
Ethanol 5% 3 hrs, larvae L3		28
Ethanol 10% 3 hrs, larvae L3		23
Caffeine 1.5 mg/ml 4 hrs, larvae L3		26
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		70
Caffeine 25 mg/ml 48 hrs, 4-day adult		40
Paraquat 5 mM 48 hrs, 4-day adult		66
Paraquat 10 mM 48 hrs, 4-day adult		66
Rotenone 2 μg 12 hrs, larvae L3		27
Rotenone 8 μg 12 hrs, larvae L3		20
Expression Level Scale		Very low Low Moderate Moderately high High

Linear, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		(36)
cold shock, 4-day adult		(29)
heat shock, 4-day adult		(40)
Cadmium 50 mM 6 hrs, larvae L3		21
Cadmium 50 mM 12 hrs, larvae L3		(31)
Cadmium 50 mM 48 hrs, 4-day adult		(41)
Cadmium 100 mM 48 hrs, 4-day adult		(77)
Copper 0.5 mM 12 hrs, larvae L3		21
Copper 15 mM 48 hrs, 4-day adult		(50)
Zinc 5 mM 12 hrs, larvae L3		19
Zinc 4.5 mM 48 hrs, 4-day adult		(44)
Ethanol 2.5% 3 hrs, larvae L3		(67)
Ethanol 5% 3 hrs, larvae L3		(28)
Ethanol 10% 3 hrs, larvae L3		23
Caffeine 1.5 mg/ml 4 hrs, larvae L3		26
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		(70)
Caffeine 25 mg/ml 48 hrs, 4-day adult		(40)
Paraquat 5 mM 48 hrs, 4-day adult		(66)
Paraquat 10 mM 48 hrs, 4-day adult		(66)
Rotenone 2 μg 12 hrs, larvae L3		(27)
Rotenone 8 μg 12 hrs, larvae L3		20
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		36
cold shock, 4-day adult		29
heat shock, 4-day adult		40
Cadmium 50 mM 6 hrs, larvae L3		21
Cadmium 50 mM 12 hrs, larvae L3		31
Cadmium 50 mM 48 hrs, 4-day adult		41
Cadmium 100 mM 48 hrs, 4-day adult		77
Copper 0.5 mM 12 hrs, larvae L3		21
Copper 15 mM 48 hrs, 4-day adult		50
Zinc 5 mM 12 hrs, larvae L3		19
Zinc 4.5 mM 48 hrs, 4-day adult		44
Ethanol 2.5% 3 hrs, larvae L3		67
Ethanol 5% 3 hrs, larvae L3		28
Ethanol 10% 3 hrs, larvae L3		23
Caffeine 1.5 mg/ml 4 hrs, larvae L3		26
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		70
Caffeine 25 mg/ml 48 hrs, 4-day adult		40
Paraquat 5 mM 48 hrs, 4-day adult		66
Paraquat 10 mM 48 hrs, 4-day adult		66
Rotenone 2 μg 12 hrs, larvae L3		27
Rotenone 8 μg 12 hrs, larvae L3		20
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		36
cold shock, 4-day adult		29
heat shock, 4-day adult		40
Cadmium 50 mM 6 hrs, larvae L3		21
Cadmium 50 mM 12 hrs, larvae L3		31
Cadmium 50 mM 48 hrs, 4-day adult		41
Cadmium 100 mM 48 hrs, 4-day adult		77
Copper 0.5 mM 12 hrs, larvae L3		21
Copper 15 mM 48 hrs, 4-day adult		50
Zinc 5 mM 12 hrs, larvae L3		19
Zinc 4.5 mM 48 hrs, 4-day adult		44
Ethanol 2.5% 3 hrs, larvae L3		67
Ethanol 5% 3 hrs, larvae L3		28
Ethanol 10% 3 hrs, larvae L3		23
Caffeine 1.5 mg/ml 4 hrs, larvae L3		26
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		70
Caffeine 25 mg/ml 48 hrs, 4-day adult		40
Paraquat 5 mM 48 hrs, 4-day adult		66
Paraquat 10 mM 48 hrs, 4-day adult		66
Rotenone 2 μg 12 hrs, larvae L3		27
Rotenone 8 μg 12 hrs, larvae L3		20
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		36
cold shock, 4-day adult		29
heat shock, 4-day adult		40
Cadmium 50 mM 6 hrs, larvae L3		21
Cadmium 50 mM 12 hrs, larvae L3		31
Cadmium 50 mM 48 hrs, 4-day adult		41
Cadmium 100 mM 48 hrs, 4-day adult		77
Copper 0.5 mM 12 hrs, larvae L3		21
Copper 15 mM 48 hrs, 4-day adult		50
Zinc 5 mM 12 hrs, larvae L3		19
Zinc 4.5 mM 48 hrs, 4-day adult		44
Ethanol 2.5% 3 hrs, larvae L3		67
Ethanol 5% 3 hrs, larvae L3		28
Ethanol 10% 3 hrs, larvae L3		23
Caffeine 1.5 mg/ml 4 hrs, larvae L3		26
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		70
Caffeine 25 mg/ml 48 hrs, 4-day adult		40
Paraquat 5 mM 48 hrs, 4-day adult		66
Paraquat 10 mM 48 hrs, 4-day adult		66
Rotenone 2 μg 12 hrs, larvae L3		27
Rotenone 8 μg 12 hrs, larvae L3		20
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		(36)
cold shock, 4-day adult		29
heat shock, 4-day adult		(40)
Cadmium 50 mM 6 hrs, larvae L3		21
Cadmium 50 mM 12 hrs, larvae L3		31
Cadmium 50 mM 48 hrs, 4-day adult		(41)
Cadmium 100 mM 48 hrs, 4-day adult		(77)
Copper 0.5 mM 12 hrs, larvae L3		21
Copper 15 mM 48 hrs, 4-day adult		(50)
Zinc 5 mM 12 hrs, larvae L3		19
Zinc 4.5 mM 48 hrs, 4-day adult		(44)
Ethanol 2.5% 3 hrs, larvae L3		(67)
Ethanol 5% 3 hrs, larvae L3		28
Ethanol 10% 3 hrs, larvae L3		23
Caffeine 1.5 mg/ml 4 hrs, larvae L3		26
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		(70)
Caffeine 25 mg/ml 48 hrs, 4-day adult		(40)
Paraquat 5 mM 48 hrs, 4-day adult		(66)
Paraquat 10 mM 48 hrs, 4-day adult		(66)
Rotenone 2 μg 12 hrs, larvae L3		27
Rotenone 8 μg 12 hrs, larvae L3		20
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		36
cold shock, 4-day adult		29
heat shock, 4-day adult		40
Cadmium 50 mM 6 hrs, larvae L3		21
Cadmium 50 mM 12 hrs, larvae L3		31
Cadmium 50 mM 48 hrs, 4-day adult		41
Cadmium 100 mM 48 hrs, 4-day adult		77
Copper 0.5 mM 12 hrs, larvae L3		21
Copper 15 mM 48 hrs, 4-day adult		50
Zinc 5 mM 12 hrs, larvae L3		19
Zinc 4.5 mM 48 hrs, 4-day adult		44
Ethanol 2.5% 3 hrs, larvae L3		67
Ethanol 5% 3 hrs, larvae L3		28
Ethanol 10% 3 hrs, larvae L3		23
Caffeine 1.5 mg/ml 4 hrs, larvae L3		26
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		70
Caffeine 25 mg/ml 48 hrs, 4-day adult		40
Paraquat 5 mM 48 hrs, 4-day adult		66
Paraquat 10 mM 48 hrs, 4-day adult		66
Rotenone 2 μg 12 hrs, larvae L3		27
Rotenone 8 μg 12 hrs, larvae L3		20
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		36
cold shock, 4-day adult		29
heat shock, 4-day adult		40
Cadmium 50 mM 6 hrs, larvae L3		21
Cadmium 50 mM 12 hrs, larvae L3		31
Cadmium 50 mM 48 hrs, 4-day adult		41
Cadmium 100 mM 48 hrs, 4-day adult		77
Copper 0.5 mM 12 hrs, larvae L3		21
Copper 15 mM 48 hrs, 4-day adult		50
Zinc 5 mM 12 hrs, larvae L3		19
Zinc 4.5 mM 48 hrs, 4-day adult		44
Ethanol 2.5% 3 hrs, larvae L3		67
Ethanol 5% 3 hrs, larvae L3		28
Ethanol 10% 3 hrs, larvae L3		23
Caffeine 1.5 mg/ml 4 hrs, larvae L3		26
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		70
Caffeine 25 mg/ml 48 hrs, 4-day adult		40
Paraquat 5 mM 48 hrs, 4-day adult		66
Paraquat 10 mM 48 hrs, 4-day adult		66
Rotenone 2 μg 12 hrs, larvae L3		27
Rotenone 8 μg 12 hrs, larvae L3		20
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Treatment		Expression Level
extended cold, 4-day adult
cold shock, 4-day adult
heat shock, 4-day adult
Cadmium 50 mM 6 hrs, larvae L3
Cadmium 50 mM 12 hrs, larvae L3
Cadmium 50 mM 48 hrs, 4-day adult
Cadmium 100 mM 48 hrs, 4-day adult
Copper 0.5 mM 12 hrs, larvae L3
Copper 15 mM 48 hrs, 4-day adult
Zinc 5 mM 12 hrs, larvae L3
Zinc 4.5 mM 48 hrs, 4-day adult
Ethanol 2.5% 3 hrs, larvae L3
Ethanol 5% 3 hrs, larvae L3
Ethanol 10% 3 hrs, larvae L3
Caffeine 1.5 mg/ml 4 hrs, larvae L3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
Caffeine 25 mg/ml 48 hrs, 4-day adult
Paraquat 5 mM 48 hrs, 4-day adult
Paraquat 10 mM 48 hrs, 4-day adult
Rotenone 2 μg 12 hrs, larvae L3
Rotenone 8 μg 12 hrs, larvae L3

Expression Clusters

A cluster of genes with similar mRNA expression dynamics across development.

External Data & Images

cell cycle defective | recessive

BDGP expression data

•

LD22943

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0002914

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG9045-RA

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

lethal

Myb^MH107

lethal, with Scer\GAL4^Act5C.PI

Myb^Δ.Scer\UAS

lethal | heat sensitive

lethal | heat sensitive, with Scer\GAL4^Act5C.PI

Myb^Scer\UAS.cFa

lethal | larval stage | recessive

lethal | maternal effect | recessive | heat sensitive

Myb¹

lethal | recessive

Myb^MH107

lethal | recessive | heat sensitive

viable, with Scer\GAL4^pnr-MD237

Myb^GD4472

Other Phenotypes

Allele

Myb^MH107

cytokinesis defective

Myb^MH107

increased cell death

increased cell death, with Scer\GAL4^en-e16E

increased cell death, with Scer\GAL4^sd-SG29.1

mitotic cell cycle defective

mitotic cell cycle defective, with Myb^MH107, Scer\GAL4^da.G32

Myb^{Nterm.Scer\UAS.T:Avic\GFP-EGFP}

mitotic cell cycle defective, with Myb^{Nterm.Scer\UAS.T:Avic\GFP-EGFP}, Scer\GAL4^da.G32

Myb^MH107

visible, with Scer\GAL4^sd-SG29.1

Myb^Scer\UAS.cFa

visible | adult stage, with Scer\GAL4^da.G32

Myb^{Cterm.Scer\UAS.T:Avic\GFP-EGFP}

visible | dominant

Myb^ΔC.GMR

visible | recessive

wild-type

Phenotype manifest in

Allele

abdomen

abdomen (with Df(1)sd72a)

adult abdomen | maternal effect

Myb¹

adult abdominal segment

Myb¹

adult abdominal sensillum

Myb¹

adult cuticle

adult cuticle, with Scer\GAL4^da.G32

Myb^{Cterm.Scer\UAS.T:Avic\GFP-EGFP}

adult cuticle (with Df(1)sd72a)

Myb²

cell cycle

Myb²

cell cycle (with Df(1)sd72a)

Myb²

centrosome

centrosome (with Df(1)sd72a)

Myb²

chorion | somatic clone

Myb^MH107

chromosome

Myb^MH107

condensed nuclear chromosome

Myb^MH107

crystal cell

Myb^MH107

dorsal appendage | somatic clone

Myb^MH107

embryo

Myb¹

embryonic/larval lymph gland

Myb^MH107

embryonic/larval salivary gland, with Scer\GAL4^fkh.PB

Myb^Δ.Scer\UAS

embryonic/larval salivary gland, with Scer\GAL4^sd-SG29.1

eye

Myb^ΔC.GMR

eye disc

Myb^ΔC.GMR

eye photoreceptor cell

Myb^ΔC.GMR

G2 phase

Myb^MH107

haltere disc, with Scer\GAL4^en-e16E

Myb^Δ.Scer\UAS

hemocyte & nucleus

Myb^MH107

histoblast

Myb²

histoblast (with Df(1)sd72a)

macrochaeta

Myb¹

macrochaeta & wing | maternal effect

Myb¹

microchaeta

Myb¹

microchaeta & adult abdomen

microchaeta & wing | maternal effect

Myb¹

mitosis & nuclear chromosome

mitosis & nuclear chromosome (with Df(1)sd72a)

Myb²

mitotic anaphase

Myb^MH107

mitotic cell cycle

mitotic cell cycle, with Scer\GAL4^en-e16E

mitotic cell cycle, with Scer\GAL4^fkh.PB

Myb^Δ.Scer\UAS

mitotic cell cycle, with Scer\GAL4^sd-SG29.1

mitotic cell cycle (with Df(1)sd72a)

Myb²

mitotic prophase

Myb²

mitotic prophase (with Df(1)sd72a)

Myb²

M phase of mitotic cell cycle

Myb^MH107

neuroblast

Myb^MH107

nuclear chromosome

Myb¹

ommatidium

Myb^ΔC.GMR

plasmatocyte

Myb^MH107

rhabdomere

Myb^ΔC.GMR

S phase

Myb^MH107

spindle

Myb^MH107

ventral thoracic disc, with Scer\GAL4^en-e16E

Myb^Scer\UAS.cFa

wing

wing, with Scer\GAL4^sd-SG29.1

Myb^Scer\UAS.cFa

wing & cell

Myb¹

wing & macrochaeta

Myb¹

wing & nucleus

Myb¹

wing | maternal effect

Myb¹

wing disc, with Scer\GAL4^en-e16E

wing disc, with Scer\GAL4^sd-SG29.1

wing hair

wing hair | ectopic

Myb¹

wing hair | precursor

Myb¹

wing hair | precursor | supernumerary

Myb¹

wing sensillum

Myb¹

wing vein

Myb¹

wing vein | maternal effect

Myb¹

Classical Alleles ( 7 )

For All Classical Alleles Show

Allele of Myb	Class	Mutagen	Stocks	Known lesion
Myb^el1	heat sensitive loss of function allele	ethyl methanesulfonate	1	Yes
Myb^el2507	hypomorphic allele - genetic evidence	ethyl methanesulfonate	1	--
Myb^G233		P-element activity	1	--
Myb¹	heat sensitive hypomorphic allele - genetic evidence	ethyl methanesulfonate	0	Yes
Myb²	heat sensitive hypomorphic allele - genetic evidence	ethyl methanesulfonate	0	Yes
Myb^MH107		Delta2-3	0	Yes
Myb^unspecified			0	--

Alleles Carried on Transgenic Constructs ( 66 )

For All Alleles Carried on Transgenic Constructs Show

Allele of Myb	Mutagen	Stocks	Known lesion
Myb^GD4472	in vitro construct - RNAi in vitro construct - regulatory fusion	2	Yes
Myb^HMS01467	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
Myb^JF02135	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
Myb^{T:Avic\GFP-EGFP}	in vitro construct - regulatory fusion in vitro construct - coding region fusion	1	Yes
Myb^+t13	in vitro construct - genomic fragment	0	Yes
Myb^+t15	in vitro construct - genomic fragment	0	Yes
Myb^+t6.6	in vitro construct - genomic fragment	0	Yes
Myb^+t6.7	in vitro construct - genomic fragment	0	Yes
Myb^+t9.0	in vitro construct - genomic fragment	0	Yes
Myb^11.6-S:S	in vitro construct - genomic fragment	0	Yes
Myb^6.7-FS-K:B	in vitro construct	0	Yes
Myb^Act5C.PH	in vitro construct - regulatory fusion	0	Yes
Myb^{C174.Scer\UAS.T:Avic\GFP-EGFP}	in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - amino acid replacement	0	Yes
Myb^CMV.cFa	in vitro construct - regulatory fusion	0	Yes
Myb^{Cterm.Scer\UAS.T:Avic\GFP-EGFP}	in vitro construct - regulatory fusion in vitro construct - coding region fusion	0	Yes
Myb^{Cterm.T:Avic\GFP-EGFP}	in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - deletion	0	Yes
Myb^{DE196.Scer\UAS.T:Avic\GFP-EGFP}	in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - amino acid replacement	0	Yes
Myb^{DKD36.Scer\UAS.T:Avic\GFP-EGFP}	in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - amino acid replacement	0	Yes
Myb^{DKD36.T:Avic\GFP-EGFP}	in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - amino acid replacement	0	Yes
Myb^{DQ617.Scer\UAS.T:Avic\GFP-EGFP}	in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - amino acid replacement	0	Yes
Myb^{DQ617.T:Avic\GFP-EGFP}	in vitro construct - regulatory fusion in vitro construct - amino acid replacement	0	Yes
Myb^dsRNA.cLa	in vitro construct - RNAi	0	Yes
Myb^dsRNA.cOa	in vitro construct - RNAi	0	Yes
Myb^dsRNA.cRa	in vitro construct - RNAi	0	Yes
Myb^{E193.Scer\UAS.T:Avic\GFP-EGFP}	in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - amino acid replacement	0	Yes
Myb^{ED94.Scer\UAS.T:Avic\GFP-EGFP}	in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - amino acid replacement	0	Yes
Myb^{EDL475.Scer\UAS.T:Avic\GFP-EGFP}	in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - amino acid replacement	0	Yes
Myb^{EDL475.T:Avic\GFP-EGFP}	in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - amino acid replacement	0	Yes
Myb^{FLK393.Scer\UAS.T:Avic\GFP-EGFP}	in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - amino acid replacement	0	Yes
Myb^{FLK393.T:Avic\GFP-EGFP}	in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - amino acid replacement	0	Yes
Myb^GMR.PO	in vitro construct - regulatory fusion	0	Yes
Myb^{K172.Scer\UAS.T:Avic\GFP-EGFP}	in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - amino acid replacement	0	Yes
Myb^KK100767	in vitro construct - RNAi in vitro construct - regulatory fusion	0	Yes
Myb^{KW606.Scer\UAS.T:Avic\GFP-EGFP}	in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - amino acid replacement	0	Yes
Myb^{KW606.T:Avic\GFP-EGFP}	in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - amino acid replacement	0	Yes
Myb^{MtnA.C.T:Avic\GFP}	in vitro construct - regulatory fusion in vitro construct - coding region fusion	0	Yes
Myb^{MtnA.N.T:Avic\GFP}	in vitro construct - regulatory fusion in vitro construct - coding region fusion	0	Yes
Myb^{N180.Scer\UAS.T:Avic\GFP-EGFP}	in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - amino acid replacement	0	Yes
Myb^NIG.9045R	in vitro construct - RNAi in vitro construct - regulatory fusion	0	Yes
Myb^{Nterm.Scer\UAS.T:Avic\GFP-EGFP}	in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - deletion	0	Yes
Myb^{PP374.Scer\UAS.T:Avic\GFP-EGFP}	in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - amino acid replacement	0	Yes
Myb^{PP374.T:Avic\GFP-EGFP}	in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - amino acid replacement	0	Yes
Myb^{RK523.Scer\UAS.T:Avic\GFP-EGFP}	in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - amino acid replacement	0	Yes
Myb^{RK523.T:Avic\GFP-EGFP}	in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - amino acid replacement	0	Yes
Myb^Scer\UAS.cDa	in vitro construct - regulatory fusion	0	Yes
Myb^Scer\UAS.cFa	in vitro construct - regulatory fusion	0	Yes
Myb^Scer\UAS.cMa	in vitro construct - regulatory fusion	0	Yes
Myb^{Scer\UAS.N.T:Avic\GFP-EGFP}	in vitro construct - regulatory fusion in vitro construct - coding region fusion	0	Yes
Myb^{Scer\UAS.N.T:Disc\RFP}	in vitro construct - regulatory fusion in vitro construct - coding region fusion	0	Yes
Myb^{Scer\UAS.T:Avic\GFP-EGFP}	in vitro construct - regulatory fusion in vitro construct - coding region fusion	0	Yes
Myb^{Scer\UAS.T:Hsap\MYC}	in vitro construct - regulatory fusion in vitro construct - coding region fusion	0	Yes
Myb^{SPS389.Scer\UAS.T:Avic\GFP-EGFP}	in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - amino acid replacement	0	Yes
Myb^{STP410.Scer\UAS.T:Avic\GFP-EGFP}	in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - amino acid replacement	0	Yes
Myb^t9.8	in vitro construct - genomic fragment	0	Yes
Myb^{TP435.Scer\UAS.T:Avic\GFP-EGFP}	in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - amino acid replacement	0	Yes
Myb^{TPT445.Scer\UAS.T:Avic\GFP-EGFP}	in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - amino acid replacement	0	Yes
Myb^{TPT445.T:Avic\GFP-EGFP}	in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - amino acid replacement	0	Yes
Myb^{Δ.Scer\UAS.T:Hsap\MYC}	in vitro construct - regulatory fusion in vitro construct - coding region fusion	0	Yes
Myb^Δ.Scer\UAS	in vitro construct - regulatory fusion	0	Yes
Myb^{Δ239-657.Act5C}	in vitro construct - deletion	0	Yes
Myb^{Δ316-657.Act5C}	in vitro construct - deletion	0	Yes
Myb^{Δ411-499.Act5C}	in vitro construct - deletion	0	Yes
Myb^{Δ416-657.Act5C}	in vitro construct - deletion	0	Yes
Myb^{Δ519-531.Act5C}	in vitro construct - deletion	0	Yes
Myb^{Δ531-657.Act5C}	in vitro construct - deletion	0	Yes
Myb^ΔC.GMR	in vitro construct - deletion	0	Yes

Aneuploid Aberrations

Disrupted in

Df(1)EM43

(Katzen et al., 1998, Katzen, 1990, Katzen and Bishop, 1996)

Df(1)Exel6251

(Ryder, 2004.4.26)

Df(1)sd72b

Duplicated in

Dp(1;4)r⁺l

Dp(1;Y)shi⁺1

(Katzen, 1990, Katzen and Bishop, 1996)

Not disrupted in

Df(1)19

(Katzen, 1990, Katzen and Bishop, 1996)

Dp(1;4)r⁺m

Dp(1;4)r⁺s

Not duplicated in

Dp(1;4)r⁺m

Dp(1;4)r⁺s

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

characterization construct

P{UAS-EGFP-Myb.Cterm}

Insertions

Type of insertions

Name

Expression data

insertion of mobile activating element

P{EP}Myb^G233

Gene Ontology: Function, Process & Cellular Component ( 25 unique terms )

Terms Based on Experimental Evidence ( 23 terms )

Molecular Function

CV term

References

DNA binding

inferred from direct assay

(Beall et al., 2002)

Biological Process

CV term

References

cell cycle

inferred from genetic interaction with cdc2

inferred from genetic interaction with CycB

inferred from genetic interaction with nej

inferred from genetic interaction with stg

inferred from mutant phenotype

cell proliferation

inferred from mutant phenotype

(Fung et al., 2002)

centriole replication

inferred from mutant phenotype

centrosome cycle

inferred from mutant phenotype

(Fung et al., 2002)

centrosome organization

inferred from mutant phenotype

eggshell chorion gene amplification

chromosome condensation

inferred from mutant phenotype

(Manak et al., 2007)

cytokinesis

inferred from mutant phenotype

(Manak et al., 2002)

inferred from mutant phenotype

(Beall et al., 2002)

Golgi organization

inferred from mutant phenotype

(Manak et al., 2002, Goshima et al., 2007)

mitosis

inferred from mutant phenotype

mitotic cell cycle

inferred from direct assay

(Wen et al., 2008)

mitotic spindle organization

inferred from mutant phenotype

negative regulation of cell size

inferred from mutant phenotype

positive regulation of cell proliferation

inferred from mutant phenotype

positive regulation of mitotic cell cycle

inferred from mutant phenotype

regulation of mitosis

inferred from direct assay

(Wen et al., 2008)

inferred from genetic interaction with nej

inferred from mutant phenotype

regulation of transcription, DNA-dependent

inferred from direct assay

spindle organization

inferred from mutant phenotype

(Manak et al., 2002)

Cellular Component

CV term

(Beall et al., 2002, Lewis et al., 2004, Korenjak et al., 2004)

References

Myb complex

inferred from direct assay

nuclear euchromatin

inferred from direct assay

(Manak et al., 2007, Scaria et al., 2008)

nucleus

inferred from direct assay

polytene chromosome

inferred from direct assay

(Beall et al., 2007)

Terms Based on Predictions or Assertions ( 6 terms )

Molecular Function

CV term

(FlyBase Curators et al., 2004-)

References

chromatin binding

inferred from electronic annotation with InterPro:IPR001005

DNA binding

inferred from sequence or structural similarity with UniProtKB:P01103

(FlyBase, 1992-)

non-traceable author statement

(Swiss-Prot Project Members, 1987.3.20)

sequence-specific DNA binding transcription factor activity

traceable author statement

(Lee and Orr-Weaver, 2003)

Biological Process

CV term

eggshell chorion gene amplification

References

traceable author statement

(Lee and Orr-Weaver, 2003)

regulation of transcription, DNA-dependent

inferred from sequence or structural similarity with UniProtKB:P01103

(FlyBase, 1992-)

Cellular Component

CV term

(Swiss-Prot Project Members, 1987.3.20)

References

nucleus

inferred from sequence or structural similarity with UniProtKB:P01103

(FlyBase, 1992-)

non-traceable author statement

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Interactions & Pathways

Summary of Physical Interactions

protein-protein

Interacting group

Assay

References

Myb - Caf1

anti tag coimmunoprecipitation, western blot

Myb - lin-52

anti tag coimmunoprecipitation, western blot, molecular sieving

(Lewis et al., 2012)

Myb - mip120

anti tag coimmunoprecipitation, western blot

Myb - mip130

anti tag coimmunoprecipitation, western blot

Myb - mip40

anti tag coimmunoprecipitation, western blot

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

Myb allele

Gene

References

nej

stg

(Fitzpatrick et al., 2002)

Myb^el2507

bon

(Nomura et al., 2004)

External Data

BioGRID - A database of protein and genetic interactions

•

58891

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0002914

InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets.

•

32543

SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores.

•

P04197

Orthologs

OrthoDB Orthologs (31) - based on analysis using Dmel annotation version 5.41

OrthoDB Ortholog Groups

OrthoDB orthology group searches

(Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288)

Drosophila inclusive ortholog search

EOG6FTWK4

Dipteran inclusive ortholog search

EOG62561X

Insect inclusive ortholog search

EOG6V6XCP

Arthropod inclusive ortholog search

EOG659ZXJ

Metazoa inclusive ortholog search

No orthologs identified

Orthologs in Drosophila Species (EOG6FTWK4)

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA21500

Drosophila persimilis

Dper\GL24710

Drosophila willistoni

Dwil\GK16252

Drosophila virilis

Dvir\GJ18431

Drosophila grimshawi

Dgri\GH11855

Orthologs in non-Drosophila Dipterans (EOG62561X)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL014286

Anopheles gambiae

Malaria mosquito

Agam\AGAP008160

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ802326

Orthologs in non-Dipteran Insects (EOG6V6XCP)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Apis mellifera

Western honey bee

Amel\GB44791

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG018237

Acromyrmex echinatior

Panamanian leafcutter ant

Aech\AECH20302

Atta cephalotes

Leafcutter ant

Acep\ACEP25174

Camponotus floridanus

Florida carpenter ant

Cflo\CFLO17795

Harpegnathos saltator

Jerdons jumping ant

Hsal\HSAL18241

Linepithema humile

Argentine ant

Lhum\LH13898

Pogonomyrmex barbatus

Red harvester ant

Pbar\PB22704

Solenopsis invicta

Red fire ant

Sinv\SINV23766

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI005393

Bombyx mori

Silkmoth

Bmor\BGIBMGA013826

Bombyx mori

Silkmoth

Bmor\BGIBMGA013827

Pediculus humanus

Human body louse

Phum\PHUM518530

Pediculus humanus

Human body louse

Phum\PHUM518540

Tribolium castaneum

Red flour beetle

Tcas\TC030738

Orthologs in non-Insect Arthropods (EOG659ZXJ)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Daphnia pulex

Water flea

Dpux\STXBP2

Ixodes scapularis

Deer tick

Isca\ISCW012531

Orthologs in non-Arthropod Metazoa (None identified)

No non-Arthropod Metazoa orthologies identified

Human Orthologs (0)

Gene

OMIM

HGNC

AAA Orthologs (10) based on analysis using Dmel annotation version 4.3

Organism

Gene

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA21500

Drosophila persimilis

Dper\GL24710

Drosophila willistoni

Dwil\GK16252

Drosophila virilis

Dvir\GJ18431

Drosophila grimshawi

Dgri\GH11855

Stocks & Reagents

Stocks Listed in FlyBase ( 8 )

Bloomington

32044

w¹¹¹⁸; P{Myb-GFP.M}A9

26237

y¹ v¹; P{TRiP.JF02135}attP2

33447

w^* P{EP}Myb^G233/FM6, w¹

35053

y¹ sc^* v¹; P{TRiP.HMS01467}attP2

Kyoto

109167

w^* Myb^el1 / FM7c

109168

w^* Myb^el2507 / FM7c

VDRC

v37710

w¹¹¹⁸; P{GD4472}v37710

v37711

w¹¹¹⁸; P{GD4472}v37711

Genomic Clones ( 2 )

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones ( 170 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

LD22943

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

DRSC - Results from RNAi screens.

•

FBgn0002914

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

32543

Antibody Information

polyclonal

(Sim et al., 2012)

Other Information

Discoverer

Etymology

Identification

Relationship to Other Genes

Source for database identity of

Source for identity of: Myb CG9045

(Gong, 1999.11)

Source for database merge of

Additional comments

Other Comments

Myb is involved in centriole duplication and is required for efficient recruitment of pericentriolar material.

Loss of Myb causes mitotic delay with spindle pole abnormalities.

(Wen et al., 2008)

RNAi screen using dsRNA made from templates generated with primers directed against this gene results in the formation of monastral bipolar, long spindles, detachment of the spindle pole and few mitoses when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.

RNAi screen using dsRNA made from templates generated with primers directed against this gene in S2 cells results in the formation of normal bipolar or occasionally multipolar spindles. Subsequently one or more of the centrosomes detech from the spindle poles and wonder toward the center of the cell. In some instances, centrosomes fuse, resulting in a monastral bipolar spindle.

nej and Myb work together to promote efficient progression through mitosis.

In abdominal epidermal cell, Myb is required to sustain the appropriate rate of proliferation, to suppress formation of supernumerary centrosomes, and to maintain genomic integrity.

(Fung et al., 2002)

Myb induces expression of CycB in the eye imaginal disc.

Myb regulates the G₂/M transition by inducing CycB expression via binding to AACNG sequences in its promoter.

Mutant wing cells fail to complete the final mitosis in their developmental lineage and a subset of nuclei enter endoreplication cycles. The failure of mutant wing cells to complete the final cell division cycle is likely to be an immediate consequence of the deficiency in Myb function.

Myb is required for both mitosis and prevention of endoreplication in wing cells. Myb acts at or near the time of the G₂/M transition.

(Rosinski and Atchley, 1998)

A series of phylogenetic analyses is performed to explore the interrelationships among 42 Myb proteins from both plants and animals - a test of the null hypothesis 'Myb proteins are a homogeneous, monophyletic, evolutionary lineage'.

Using the co-transfection assay with the nej expression plasmid results demonstrate trans-activation by Myb and functional domains of Myb involved in this activity are identified.

(Hou et al., 1997)

There is a quasihydrophobic site on the protein to which the hydrophobic probe TNS (6-p-Toluidino-2-naphthalenesulfonate) binds. This binding site is found within the vicinity of Tyr and several Trp residues.

(Madan et al., 1994)

The DNA binding domain of Myb has been overexpressed in E.coli, purified, and subjected to 2D and 3D NMR experiments.

(Radha et al., 1994)

Myb is an essential gene whose function is important during most stages of development. Aspects of the mutant phenotype implicate Myb in the control of cell division.

Myb has been cloned and sequenced. It contains two regions of homology to vertebrate myb proteins. DNA-binding experiments show that the amino-terminal proximal region of homology functions as a DNA binding domain.