Gene: Dmel\Myb
Dm-Myb, Dm myb, dMyb, c-Myb, D-myb
transcription factor - Myb DNA-binding domain - required for mitosis and prevention of endoreduplication in wing cells - activates the transcription of genes involved in the G2/M phases of the cell cycle by inhibition of the highly conserved MuvB multi-protein repressor complex.
Please see the JBrowse view of Dmel\Myb for information on other features
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Gene model reviewed during 6.01
Gene model reviewed during 5.50
Annotated transcripts do not represent all supported alternative splices within 5' UTR.
Low-frequency RNA-Seq exon junction(s) not annotated.
Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.
Gene model reviewed during 5.55
Gene model reviewed during 6.29
3.2 (northern blot)
2.814 (longest cDNA)
657 (aa); 74 (kD predicted)
Component of the DREAM complex at least composed of Myb, Caf1-55, mip40, mip120, mip130, E2f2, Dp, Rbf, Rbf2, lin-52, HDAC1/Rpd3 and l(3)mbt.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Myb using the Feature Mapper tool.
Comment: maternally deposited
The 3.2 kb Myb transcript is detected throughout development. Myb transcript is detected mainly in proliferating tissues, though expression is seen at lower levels in some post-mitotic tissues. Expression is uniform and high in preblastoderm embryos, and continues to be high during cellular blastoderm formation and germ band extension. Message is less uniform in contracted germ band embryos, with no expression seen in the amnioserosa, and low expression in the salivary glands. Later in embryogenesis, Myb transcript is high only in the CNS. In climbing third instar larvae, tissues contributing to the adult express Myb at high levels: imaginal disc, brain, ventral ganglion, testes, ovaries, and nests of imaginal cells in the gut. Expression in these tissues continues in the early pupa, with additional expression detected in histoblasts and epithelial cells of the developing imaginal gut. Myb levels decline in everted imaginal tissues. Ovary, testis, and neural tissue continue to express Myb transcript through pupal development. Myb is detected at low levels in the brain and thoracic ganglia of adults, and expression is high in adult testes and ovaries.
GBrowse - Visual display of RNA-Seq signals
View Dmel\Myb in GBrowse 21-52
1-52.2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Duplicate transcripts identified and eliminated during the migration of annotations from the release 5 genome assembly to the release 6 assembly.
Myb is involved in centriole duplication and is required for efficient recruitment of pericentriolar material.
Loss of Myb causes mitotic delay with spindle pole abnormalities.
RNAi screen using dsRNA made from templates generated with primers directed against this gene results in the formation of monastral bipolar, long spindles, detachment of the spindle pole and few mitoses when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.
RNAi screen using dsRNA made from templates generated with primers directed against this gene in S2 cells results in the formation of normal bipolar or occasionally multipolar spindles. Subsequently one or more of the centrosomes detech from the spindle poles and wonder toward the center of the cell. In some instances, centrosomes fuse, resulting in a monastral bipolar spindle.
In abdominal epidermal cell, Myb is required to sustain the appropriate rate of proliferation, to suppress formation of supernumerary centrosomes, and to maintain genomic integrity.
Mutant wing cells fail to complete the final mitosis in their developmental lineage and a subset of nuclei enter endoreplication cycles. The failure of mutant wing cells to complete the final cell division cycle is likely to be an immediate consequence of the deficiency in Myb function.
A series of phylogenetic analyses is performed to explore the interrelationships among 42 Myb proteins from both plants and animals - a test of the null hypothesis 'Myb proteins are a homogeneous, monophyletic, evolutionary lineage'.
There is a quasihydrophobic site on the protein to which the hydrophobic probe TNS (6-p-Toluidino-2-naphthalenesulfonate) binds. This binding site is found within the vicinity of Tyr and several Trp residues.
Myb has been cloned and sequenced. It contains two regions of homology to vertebrate myb proteins. DNA-binding experiments show that the amino-terminal proximal region of homology functions as a DNA binding domain.
Considered to be the Drosophila homologue of avian c-myb. Open reading frame identified to date encodes a polypeptide exhibiting 73% homology with chicken c-myb protein.
