Gene Dmel\Atpα

General Information
Symbol	Dmel\Atpα	Species	D. melanogaster
Name	Na pump α subunit	Annotation symbol	CG5670
Feature type	protein_coding_gene	FlyBase ID	FBgn0002921
Created / Updated	2003-12-02/2003-12-02
Genomic Location
Chromosome (arm)	3R	Recombination map
Cytogenetic map	93A4-93A4	Sequence location	3R:16,774,436..16,801,022 [+]
Map ( GBrowse )
Summary Information
Automatically generated summary See sections below for more information	The gene Na pump α subunit is referred to in FlyBase by the symbol Atpα (CG5670, FBgn0002921). It has the cytological map location 93A4. Its sequence location is 3R:16774436..16801022. Its molecular function is described as: sodium:potassium-exchanging ATPase activity; hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances; cation transmembrane transporter activity; ATP binding; monovalent inorganic cation transmembrane transporter activity. It is involved in the biological processes described with 15 unique terms, many of which group under: anatomical structure development; open tracheal system development; locomotory behavior; ion transport; transport; behavior; response to abiotic stimulus; regulation of tube architecture, open tracheal system; determination of adult life span; response to mechanical stimulus; embryonic development via the syncytial blastoderm; primary metabolic process; monovalent inorganic cation transport; neuromuscular process; tissue homeostasis; response to temperature stimulus. 65 alleles are reported. The phenotypes of these alleles are annotated with 13 unique terms, many of which group under: organ system; embryonic/larval tracheal system; anatomical structure; nervous system; tracheal system; embryonic segment; embryonic ganglionic branch; embryonic dorsal trunk; adult brain; plasma membrane part; embryonic trachea; adult segment. It has 8 annotated transcripts and 8 annotated polypeptides.
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	Atpα: (Na+ + K+) ATPase α subunit Structural gene for the α or catalytic subunit of Na+ K+ ATPase (ouabain sensitive). Immunofluorescence microscopy with a monoclonal antibody demonstrates high concentrations of (ATPase in adult muscle, nervous tissue, and Malpighian tubules; strong immunofluorescence observed in brain, optic lobes, photoreceptor cells of retina, and ventral thoracic neuromere. Flies heterozygous for Df(3R)r1-G6 which lacks Atpα, are sluggish and less tolerant of physical stress than normal.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 93A4-93A4 Limits computationally determined from genome sequence between P{EP}Synd^EP409 and P{lacW}l(3)j5C7^j5C7
Experimentally Determined Cytological Location
Cytogenetic map Notes References 93A7-93B1 (determined by in situ hybridisation) (Ranz et al., 2001) 93B2-93B2 (Sun, 1998.1.25) 93B1-93B2 (determined by in situ hybridisation) (Spradling et al., 1999) 93B-93B (determined by in situ hybridisation) (Feng et al., 1997) 93B1-93B2 (determined by in situ hybridisation) (Schwarz, 1996) 93B1-93B2 (determined by in situ hybridisation) (Perrimon et al., 1996) 93B1-93B2 (determined by in situ hybridisation) (Schubiger et al., 1994) 93B1-93B2 93B1--3 93B2--3 (BDGP Project Members, 1994-1999) 93B1-93B2 (determined by in situ hybridisation) (Berg and Spradling, 1991) 93B-93B (determined by in situ hybridisation) (Lebovitz et al., 1989)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology) References In direction of increasing cytology: Atpα+ Calx+ (Schwarz, 1996, Schwarz and Benzer, 1997) Gene Order (overall orientation not stated) References
Gene Model & Products
Please see the GBrowse view of Dmel\Atpα for information on other features
Comments on Gene Model
	EST data support existence of multiple transcripts due to variable use of mini-exons . It is not known how many of the possible combinations of variable exons occur in vivo and all possible combinations have not been annotated. DGC clone appears problematic (HL02687): genomically primed EST data support existence of multiple transcripts due to variable use of 5' exons
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Atpalpha-RC FBtr0089509 NM_169938 3637 1002 Atpalpha-RD FBtr0089512 NM_169939 5088 839 Atpalpha-RE FBtr0089513 NM_206528 3902 1002 Atpalpha-RF FBtr0089514 NM_206527 3637 1002 Atpalpha-RG FBtr0089515 NM_206526 3637 1002 Atpalpha-RH FBtr0089516 NM_206525 3848 1002 Atpα-RA FBtr0089510 NM_169936 3618 1041 Atpα-RB FBtr0089511 NM_169937 3848 1002
Additional Transcript Data & Comments
Reported size (kB)	4.8, 3.8 (northern blot); 3.6 (unknown) (Lebovitz et al., 1989) 6.5, 4.5, 4.2 (northern blot) (Varadi et al., 1989)
Comments	Several polyadenylation sites are thought to be used for Atpα transcription. (Lebovitz et al., 1989, Lebovitz et al., 1989)
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein Atpalpha-PA FBpp0088502 115.6 1041 5.40 NP_732572 AAF55825 Atpalpha-PB FBpp0088503 111.1 1002 5.25 NP_732573 AAF55828 Atpalpha-PC FBpp0088501 111.1 1002 5.25 NP_732574 AAF55827 Atpalpha-PD FBpp0088504 92.0 839 5.33 NP_732575 AAF55826 Atpalpha-PE FBpp0088984 111.1 1002 5.25 NP_996250 AAS65183 Atpalpha-PF FBpp0088983 111.2 1002 5.20 NP_996249 AAS65184 Atpalpha-PG FBpp0088982 111.3 1002 5.26 NP_996248 AAS65186 Atpalpha-PH FBpp0088981 111.2 1002 5.20 NP_996247 AAS65185
Additional Polypeptide Data & Comments
Reported size (kD)	1038 (aa); 100 (kD observed) (Lebovitz et al., 1989)
Comments
External Data
Linkouts	PANTHER - Protein classification by function, families, and pathways • FBgn0002921
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • ATPase, P-type, K/Mg/Cd/Cu/Zn/Na/Ca/Na/H-transporter (IPR001757) ATPase, P-type cation-transporter, N-terminal (IPR004014) ATPase, P-type cation exchange, alpha subunit, eukaryotic (IPR005775) Haloacid dehalogenase-like hydrolase (IPR005834) ATPase, P-type cation-transporter, C-terminal (IPR006068) ATPase, P-type cation exchange, alpha subunit (IPR006069) E1-E2 ATPase-associated region (IPR008250)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA567433 AA567605 AA568019 AA696622 AA697459 AA697536 AA697647 AA697851 AA698112 AA803094 AE003732 AAF55825 AE003732 AAF55826 AE003732 AAF55827 AE003732 AAF55828 AE003732 AAS65183 AE003732 AAS65184 AE003732 AAS65185 AE003732 AAS65186 AF044974 AAC05260 AI062270 AI064058 AI064208 AI238454 AI238532 AI297245 AI387534 AI388009 AI388506 AI389087 AI389404 AI403934 AI404144 AI405087 AI513383 AI514911 AI533575 AI534114 AQ026199 AW940908 AY069184 AAL39329 AY174097 AAO33930 AY174097 AAO33931 AY174097 AAO33932 AY174097 AAO33933 AY174098 AAO33929 BI167367 BI171115 BI173864 BI212319 BI215670 BI216833 BI228524 BI229294 BI229809 BI230873 BI231190 BI232793 BI363743 BI365111 BI366040 BI370233 BI371006 BI371327 BI373505 BI484168 BI564159 BI564539 BI568380 BI582580 BI583588 BI588088 BI593294 BI604470 BI605107 BI607541 BI626039 BI631974 BI634818 BP540775 BP540776 BP543143 BP543144 BP550090 BP550091 BP555783 BP555784 BP558967 CK657937 CK659329 CL610121 CO156107 CO156467 CO178345 CO180464 CO182069 CO189543 CO190405 CO194532 CO195430 CO267226 CO268361 CO271128 CO272313 CO273063 CO273348 CO278250 CO279492 CO280364 CO280430 CO281526 CO285196 CO286069 CO289936 CO291135 CO294329 CO294712 CO294945 CO299639 CO299946 CO300548 CO300644 CO301514 CO303267 CO305530 CO305545 CO306863 CO310977 CO313270 CO314632 CO314777 CO317480 CO317902 CO318776 CO318839 CO319855 CO322134 CO324532 CO324815 CO325046 CO325934 CO326771 CO327291 CO327722 CO330986 CO331892 CO341521 CO341934 CO343442 CS133205 CZ467671 CZ467672 CZ471181 CZ473046 CZ473769 CZ473770 CZ474009 CZ475611 CZ476343 CZ476595 CZ481484 CZ481485 EC051490 EC062777 EC063385 EC063732 EC064126 EC066637 EC066726 EC066912 EC066925 EC067096 EC074876 EC076840 EC077072 EC077323 EC079417