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Gene Dmel\nec

General Information
Symbol	Dmel\nec	Species	D. melanogaster
Name	necrotic	Annotation symbol	CG1857
Feature type	protein_coding_gene	FlyBase ID	FBgn0002930
Gene Model Status	Current	Stock availability	8 publicly available
Also Known As	Spn43Ac, SER3
Genomic Location
Chromosome (arm)	2R	Recombination map
Cytogenetic map	42F3-42F3	Sequence location	2R:3,043,982..3,045,746 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene necrotic is referred to in FlyBase by the symbol Dmel\nec (CG1857, FBgn0002930). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: serine-type endopeptidase inhibitor activity. There is experimental evidence that it is involved in the biological process: immune response; negative regulation of Toll signaling pathway; antifungal humoral response; regulation of proteolysis. 36 alleles are reported. The phenotypes of these alleles are annotated with: adult epidermis; adult cuticle; adult integumentary system; adult abdomen; joint. It has one annotated transcript and one annotated polypeptide. Protein features are: Protease inhibitor I4, serpin, conserved site; Serpin domain; Serpin family. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of extremely low expression. Peak expression observed at stages throughout the pupal period, in stages of adults of both sexes. Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, adult crop, adult heart, larval/adult fat body, adult spermathecae, adult carcass. Expression at moderate levels in the following post-embryonic organs or tissues: adult central nervous system, larval/adult hindgut. Comments on Affy2 ProbeSet: ProbeSet 1636653_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of nec. Gene sequence location is 2R:3043982..3045746.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02	Controlled Vocabulary Terms negative regulation of endopeptidase activity
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 42F3-42F3   Limits computationally determined from genome sequence between P{lacW}vimark16722 and P{lacW}cosk16101
Experimentally Determined Cytological Location
Cytogenetic map Notes References 43A1-43A2   (determined by in situ hybridisation)   (Gubb, 1998.3.20)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\nec for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.48
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) nec-RA FBtr0089052  NM_080112   1633   476
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein nec-PA   FBpp0088123   53.7   476   7.41     NP_524851   AAF59285
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Serpin family (IPR000215) Serpin domain (IPR023796) Protease inhibitor I4, serpin, conserved site (IPR023795)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA697593   AA698400   AA699031   AE013599 AAF59285   AI062016   AI109059   AI113685   AI386991   AI387159   AI387864   AI389919   AI402196   AI403022   AI403391   AI404272   AI513684   AI513761   AJ245444 CAB56697   AX100272   AY102659 AAM27488   AY349666 AAQ64949   AY349667 AAQ64950   AY349668 AAQ64951   AY349669 AAQ64952   AY349670 AAQ64953   AY349671 AAQ64954   AY349672 AAQ64955   AY349673 AAQ64956   AY349674 AAQ64957   AY349675 AAQ64958   BF490505   CA803798   CK659310   CK659443   CO182203   CO282347   CO302539   CO312438   CO316779   CO326979   EC054735   EC062043   EC074383   EC076224   EC238502   EC246222   EC258765   EV578579
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q7JWX3 Q6V6U1 Q6V6V0 Q6V6U3 Q6V6U2 Q6V6U8 Q9U5W7 Q6V6U7 Q6V6U4 Q6V6U5 Q6V6U6 Q6V6U9
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
extracellular space inferred from direct assay (Levashina et al., 1999)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, adult crop, adult heart, larval/adult fat body, adult spermathecae, adult carcass. Expression at moderate levels in the following post-embryonic organs or tissues: adult central nervous system, larval/adult hindgut. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    16.975 Larval Midgut    2.4 Larval Hindgut    106.7 Larval Malpighian Tubules    5.9 Larval Fat Body    1114.9 Larval Salivary Gland    35.6 Larval Trachea    42.425 Larval Carcass    47.8 Adult Head    1031.2 Adult Eye    619.1 Adult Brain    273 Adult Thoracic-Abdominal Ganglion    335.8 Adult Crop    587.7 Adult Midgut    25 Adult Hindgut    419.1 Adult Malpighian Tubules    25.7 Adult Fat Body    1884.1 Adult Salivary Gland    72.1 Adult Heart    1113.9 Adult VirginFemale Spermatheca    1157.4 Adult InseminatedFemale Spermatheca    883.6 Adult Ovary    4 Adult Testis    63.9 Adult Male Accessory Gland    90.5 Adult Carcass    1204.5 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    16.975 Larval Midgut    2.4 Larval Hindgut    106.7 Larval Malpighian Tubules    5.9 Larval Fat Body  (1114.9) Larval Salivary Gland    35.6 Larval Trachea    42.425 Larval Carcass    47.8 Adult Head  (1031.2) Adult Eye  (619.1) Adult Brain    273 Adult Thoracic-Abdominal Ganglion    335.8 Adult Crop  (587.7) Adult Midgut    25 Adult Hindgut    419.1 Adult Malpighian Tubules    25.7 Adult Fat Body  (1884.1) Adult Salivary Gland    72.1 Adult Heart  (1113.9) Adult VirginFemale Spermatheca  (1157.4) Adult InseminatedFemale Spermatheca  (883.6) Adult Ovary    4 Adult Testis    63.9 Adult Male Accessory Gland    90.5 Adult Carcass  (1204.5) Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    16.975 Larval Midgut    2.4 Larval Hindgut    106.7 Larval Malpighian Tubules    5.9 Larval Fat Body  (1114.9) Larval Salivary Gland    35.6 Larval Trachea    42.425 Larval Carcass    47.8 Adult Head  1031.2 Adult Eye    619.1 Adult Brain    273 Adult Thoracic-Abdominal Ganglion    335.8 Adult Crop    587.7 Adult Midgut    25 Adult Hindgut    419.1 Adult Malpighian Tubules    25.7 Adult Fat Body  (1884.1) Adult Salivary Gland    72.1 Adult Heart  (1113.9) Adult VirginFemale Spermatheca  (1157.4) Adult InseminatedFemale Spermatheca    883.6 Adult Ovary    4 Adult Testis    63.9 Adult Male Accessory Gland    90.5 Adult Carcass  (1204.5) Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    16.975 Larval Midgut    2.4 Larval Hindgut    106.7 Larval Malpighian Tubules    5.9 Larval Fat Body    1114.9 Larval Salivary Gland    35.6 Larval Trachea    42.425 Larval Carcass    47.8 Adult Head    1031.2 Adult Eye    619.1 Adult Brain    273 Adult Thoracic-Abdominal Ganglion    335.8 Adult Crop    587.7 Adult Midgut    25 Adult Hindgut    419.1 Adult Malpighian Tubules    25.7 Adult Fat Body    1884.1 Adult Salivary Gland    72.1 Adult Heart    1113.9 Adult VirginFemale Spermatheca    1157.4 Adult InseminatedFemale Spermatheca    883.6 Adult Ovary    4 Adult Testis    63.9 Adult Male Accessory Gland    90.5 Adult Carcass    1204.5 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    16.975 Larval Midgut    2.4 Larval Hindgut    106.7 Larval Malpighian Tubules    5.9 Larval Fat Body    1114.9 Larval Salivary Gland    35.6 Larval Trachea    42.425 Larval Carcass    47.8 Adult Head    1031.2 Adult Eye    619.1 Adult Brain    273 Adult Thoracic-Abdominal Ganglion    335.8 Adult Crop    587.7 Adult Midgut    25 Adult Hindgut    419.1 Adult Malpighian Tubules    25.7 Adult Fat Body    1884.1 Adult Salivary Gland    72.1 Adult Heart    1113.9 Adult VirginFemale Spermatheca    1157.4 Adult InseminatedFemale Spermatheca    883.6 Adult Ovary    4 Adult Testis    63.9 Adult Male Accessory Gland    90.5 Adult Carcass    1204.5 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    16.975 Larval Midgut    2.4 Larval Hindgut    106.7 Larval Malpighian Tubules    5.9 Larval Fat Body  (1114.9) Larval Salivary Gland    35.6 Larval Trachea    42.425 Larval Carcass    47.8 Adult Head  (1031.2) Adult Eye  619.1 Adult Brain    273 Adult Thoracic-Abdominal Ganglion    335.8 Adult Crop  587.7 Adult Midgut    25 Adult Hindgut    419.1 Adult Malpighian Tubules    25.7 Adult Fat Body  (1884.1) Adult Salivary Gland    72.1 Adult Heart  (1113.9) Adult VirginFemale Spermatheca  (1157.4) Adult InseminatedFemale Spermatheca  (883.6) Adult Ovary    4 Adult Testis    63.9 Adult Male Accessory Gland    90.5 Adult Carcass  (1204.5) Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    16.975 Larval Midgut    2.4 Larval Hindgut    106.7 Larval Malpighian Tubules    5.9 Larval Fat Body  1114.9 Larval Salivary Gland    35.6 Larval Trachea    42.425 Larval Carcass    47.8 Adult Head  1031.2 Adult Eye    619.1 Adult Brain    273 Adult Thoracic-Abdominal Ganglion    335.8 Adult Crop    587.7 Adult Midgut    25 Adult Hindgut    419.1 Adult Malpighian Tubules    25.7 Adult Fat Body  (1884.1) Adult Salivary Gland    72.1 Adult Heart  1113.9 Adult VirginFemale Spermatheca  1157.4 Adult InseminatedFemale Spermatheca    883.6 Adult Ovary    4 Adult Testis    63.9 Adult Male Accessory Gland    90.5 Adult Carcass  1204.5 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    16.975 Larval Midgut    2.4 Larval Hindgut    106.7 Larval Malpighian Tubules    5.9 Larval Fat Body    1114.9 Larval Salivary Gland    35.6 Larval Trachea    42.425 Larval Carcass    47.8 Adult Head    1031.2 Adult Eye    619.1 Adult Brain    273 Adult Thoracic-Abdominal Ganglion    335.8 Adult Crop    587.7 Adult Midgut    25 Adult Hindgut    419.1 Adult Malpighian Tubules    25.7 Adult Fat Body    1884.1 Adult Salivary Gland    72.1 Adult Heart    1113.9 Adult VirginFemale Spermatheca    1157.4 Adult InseminatedFemale Spermatheca    883.6 Adult Ovary    4 Adult Testis    63.9 Adult Male Accessory Gland    90.5 Adult Carcass    1204.5 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    1031.2   NA  Eye    619.1   NA  Brain    273   no informative data  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    335.8   NA  Crop    587.7   2.4  Midgut    no informative data   106.7  Hindgut    419.1   5.9  Malpighian Tubules    no informative data   1114.9  Fat Body    1884.1   no informative data  Salivary Gland    no informative data   NA  Heart    1113.9   42.425  Trachea    NA   NA  VirginFemale Spermatheca    1157.4   NA  InseminatedFemale Spermatheca    883.6   NA  Ovary    4   NA  Testis    63.9   NA  Male Accessory Gland    90.5   47.8  Carcass    1204.5 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    2 central nervous system, larvae L3    2 central nervous system, pupae P8    2 head, virgin 1-day female    120 head, virgin 4-day female    130 head, virgin 20-day female    331 head, mated 1-day female    98 head, mated 4-day female    104 head, mated 20-day female    248 head, mated 1-day male    154 head, mated 4-day male    141 head, mated 20-day male    212 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    4 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    18 digestive system, 4-day adult    11 digestive system, 20-day adult    18 fat body, larvae L3 wandering    117 fat body, white prepupae    38 fat body, pupae P8    79 carcass, larvae L3 wandering    8 carcass, 1-day adult    166 carcass, 4-day adult    137 carcass, 20-day adult    367 ovary, virgin 4-day female    1 ovary, mated 4-day female    0 testis, mated 4-day male    17 accessory gland, mated 4-day male    10 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    2 central nervous system, larvae L3    2 central nervous system, pupae P8    2 head, virgin 1-day female  (120) head, virgin 4-day female  (130) head, virgin 20-day female  (331) head, mated 1-day female  (98) head, mated 4-day female  (104) head, mated 20-day female  (248) head, mated 1-day male  (154) head, mated 4-day male  (141) head, mated 20-day male  (212) salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    4 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    18 digestive system, 4-day adult    11 digestive system, 20-day adult    18 fat body, larvae L3 wandering  (117) fat body, white prepupae  (38) fat body, pupae P8  (79) carcass, larvae L3 wandering    8 carcass, 1-day adult  (166) carcass, 4-day adult  (137) carcass, 20-day adult  (367) ovary, virgin 4-day female    1 ovary, mated 4-day female    0 testis, mated 4-day male    17 accessory gland, mated 4-day male    10 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    2 central nervous system, larvae L3    2 central nervous system, pupae P8    2 head, virgin 1-day female  (120) head, virgin 4-day female  (130) head, virgin 20-day female  (331) head, mated 1-day female    98 head, mated 4-day female  104 head, mated 20-day female  (248) head, mated 1-day male  (154) head, mated 4-day male  (141) head, mated 20-day male  (212) salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    4 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    18 digestive system, 4-day adult    11 digestive system, 20-day adult    18 fat body, larvae L3 wandering  (117) fat body, white prepupae    38 fat body, pupae P8    79 carcass, larvae L3 wandering    8 carcass, 1-day adult  (166) carcass, 4-day adult  (137) carcass, 20-day adult  (367) ovary, virgin 4-day female    1 ovary, mated 4-day female    0 testis, mated 4-day male    17 accessory gland, mated 4-day male    10 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    2 central nervous system, larvae L3    2 central nervous system, pupae P8    2 head, virgin 1-day female    120 head, virgin 4-day female    130 head, virgin 20-day female    331 head, mated 1-day female    98 head, mated 4-day female    104 head, mated 20-day female    248 head, mated 1-day male    154 head, mated 4-day male    141 head, mated 20-day male    212 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    4 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    18 digestive system, 4-day adult    11 digestive system, 20-day adult    18 fat body, larvae L3 wandering    117 fat body, white prepupae    38 fat body, pupae P8    79 carcass, larvae L3 wandering    8 carcass, 1-day adult    166 carcass, 4-day adult    137 carcass, 20-day adult    367 ovary, virgin 4-day female    1 ovary, mated 4-day female    0 testis, mated 4-day male    17 accessory gland, mated 4-day male    10 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    2 central nervous system, larvae L3    2 central nervous system, pupae P8    2 head, virgin 1-day female    120 head, virgin 4-day female    130 head, virgin 20-day female    331 head, mated 1-day female    98 head, mated 4-day female    104 head, mated 20-day female    248 head, mated 1-day male    154 head, mated 4-day male    141 head, mated 20-day male    212 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    4 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    18 digestive system, 4-day adult    11 digestive system, 20-day adult    18 fat body, larvae L3 wandering    117 fat body, white prepupae    38 fat body, pupae P8    79 carcass, larvae L3 wandering    8 carcass, 1-day adult    166 carcass, 4-day adult    137 carcass, 20-day adult    367 ovary, virgin 4-day female    1 ovary, mated 4-day female    0 testis, mated 4-day male    17 accessory gland, mated 4-day male    10 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    2 central nervous system, larvae L3    2 central nervous system, pupae P8    2 head, virgin 1-day female  (120) head, virgin 4-day female  (130) head, virgin 20-day female  (331) head, mated 1-day female  (98) head, mated 4-day female  (104) head, mated 20-day female  (248) head, mated 1-day male  (154) head, mated 4-day male  (141) head, mated 20-day male  (212) salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    4 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    18 digestive system, 4-day adult    11 digestive system, 20-day adult    18 fat body, larvae L3 wandering  (117) fat body, white prepupae  (38) fat body, pupae P8  (79) carcass, larvae L3 wandering    8 carcass, 1-day adult  (166) carcass, 4-day adult  (137) carcass, 20-day adult  (367) ovary, virgin 4-day female    1 ovary, mated 4-day female    0 testis, mated 4-day male    17 accessory gland, mated 4-day male    10 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    2 central nervous system, larvae L3    2 central nervous system, pupae P8    2 head, virgin 1-day female  120 head, virgin 4-day female  130 head, virgin 20-day female  (331) head, mated 1-day female    98 head, mated 4-day female  104 head, mated 20-day female  (248) head, mated 1-day male  (154) head, mated 4-day male  141 head, mated 20-day male  (212) salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    4 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    18 digestive system, 4-day adult    11 digestive system, 20-day adult    18 fat body, larvae L3 wandering  117 fat body, white prepupae    38 fat body, pupae P8    79 carcass, larvae L3 wandering    8 carcass, 1-day adult  (166) carcass, 4-day adult  137 carcass, 20-day adult  (367) ovary, virgin 4-day female    1 ovary, mated 4-day female    0 testis, mated 4-day male    17 accessory gland, mated 4-day male    10 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    2 central nervous system, larvae L3    2 central nervous system, pupae P8    2 head, virgin 1-day female    120 head, virgin 4-day female    130 head, virgin 20-day female    331 head, mated 1-day female    98 head, mated 4-day female    104 head, mated 20-day female    248 head, mated 1-day male    154 head, mated 4-day male    141 head, mated 20-day male    212 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    4 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    18 digestive system, 4-day adult    11 digestive system, 20-day adult    18 fat body, larvae L3 wandering    117 fat body, white prepupae    38 fat body, pupae P8    79 carcass, larvae L3 wandering    8 carcass, 1-day adult    166 carcass, 4-day adult    137 carcass, 20-day adult    367 ovary, virgin 4-day female    1 ovary, mated 4-day female    0 testis, mated 4-day male    17 accessory gland, mated 4-day male    10 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of extremely low expression. Peak expression observed at stages throughout the pupal period, in stages of adults of both sexes. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    1 embryo 08-10hr    2 embryo 10-12hr    7 embryo 12-14hr    9 embryo 14-16hr    12 embryo 16-18hr    13 embryo 18-20hr    44 embryo 20-22hr    49 embryo 22-24hr    34 larva L1    17 larva L2    21 larva L3 12hr old    38 larva L3 puffstage 1-2    35 larva L3 puffstage 3-6    39 larva L3 puffstage 7-9    20 white prepupae new    20 white prepupae 12hr    87 white prepupae 24hr    47 pupae 2d postWPP    45 pupae 3d postWPP    25 pupae 4d postWPP    119 adult male 01day    87 adult male 05day    87 adult male 30day    86 adult female 01day    53 adult female 05day    33 adult female 30day    57 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    1 embryo 08-10hr    2 embryo 10-12hr    7 embryo 12-14hr    9 embryo 14-16hr    12 embryo 16-18hr    13 embryo 18-20hr  (44) embryo 20-22hr  (49) embryo 22-24hr  (34) larva L1    17 larva L2    21 larva L3 12hr old  (38) larva L3 puffstage 1-2  (35) larva L3 puffstage 3-6  (39) larva L3 puffstage 7-9    20 white prepupae new    20 white prepupae 12hr  (87) white prepupae 24hr  (47) pupae 2d postWPP  (45) pupae 3d postWPP    25 pupae 4d postWPP  (119) adult male 01day  (87) adult male 05day  (87) adult male 30day  (86) adult female 01day  (53) adult female 05day  (33) adult female 30day  (57) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    1 embryo 08-10hr    2 embryo 10-12hr    7 embryo 12-14hr    9 embryo 14-16hr    12 embryo 16-18hr    13 embryo 18-20hr    44 embryo 20-22hr    49 embryo 22-24hr    34 larva L1    17 larva L2    21 larva L3 12hr old    38 larva L3 puffstage 1-2    35 larva L3 puffstage 3-6    39 larva L3 puffstage 7-9    20 white prepupae new    20 white prepupae 12hr    87 white prepupae 24hr    47 pupae 2d postWPP    45 pupae 3d postWPP    25 pupae 4d postWPP  (119) adult male 01day    87 adult male 05day    87 adult male 30day    86 adult female 01day    53 adult female 05day    33 adult female 30day    57 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    1 embryo 08-10hr    2 embryo 10-12hr    7 embryo 12-14hr    9 embryo 14-16hr    12 embryo 16-18hr    13 embryo 18-20hr    44 embryo 20-22hr    49 embryo 22-24hr    34 larva L1    17 larva L2    21 larva L3 12hr old    38 larva L3 puffstage 1-2    35 larva L3 puffstage 3-6    39 larva L3 puffstage 7-9    20 white prepupae new    20 white prepupae 12hr    87 white prepupae 24hr    47 pupae 2d postWPP    45 pupae 3d postWPP    25 pupae 4d postWPP    119 adult male 01day    87 adult male 05day    87 adult male 30day    86 adult female 01day    53 adult female 05day    33 adult female 30day    57 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    1 embryo 08-10hr    2 embryo 10-12hr    7 embryo 12-14hr    9 embryo 14-16hr    12 embryo 16-18hr    13 embryo 18-20hr    44 embryo 20-22hr    49 embryo 22-24hr    34 larva L1    17 larva L2    21 larva L3 12hr old    38 larva L3 puffstage 1-2    35 larva L3 puffstage 3-6    39 larva L3 puffstage 7-9    20 white prepupae new    20 white prepupae 12hr    87 white prepupae 24hr    47 pupae 2d postWPP    45 pupae 3d postWPP    25 pupae 4d postWPP    119 adult male 01day    87 adult male 05day    87 adult male 30day    86 adult female 01day    53 adult female 05day    33 adult female 30day    57 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    1 embryo 08-10hr    2 embryo 10-12hr    7 embryo 12-14hr    9 embryo 14-16hr    12 embryo 16-18hr    13 embryo 18-20hr  (44) embryo 20-22hr  (49) embryo 22-24hr  (34) larva L1    17 larva L2    21 larva L3 12hr old  (38) larva L3 puffstage 1-2  (35) larva L3 puffstage 3-6  (39) larva L3 puffstage 7-9    20 white prepupae new    20 white prepupae 12hr  (87) white prepupae 24hr  (47) pupae 2d postWPP  (45) pupae 3d postWPP    25 pupae 4d postWPP  (119) adult male 01day  (87) adult male 05day  (87) adult male 30day  (86) adult female 01day  (53) adult female 05day  (33) adult female 30day  (57) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    1 embryo 08-10hr    2 embryo 10-12hr    7 embryo 12-14hr    9 embryo 14-16hr    12 embryo 16-18hr    13 embryo 18-20hr    44 embryo 20-22hr    49 embryo 22-24hr    34 larva L1    17 larva L2    21 larva L3 12hr old    38 larva L3 puffstage 1-2    35 larva L3 puffstage 3-6    39 larva L3 puffstage 7-9    20 white prepupae new    20 white prepupae 12hr    87 white prepupae 24hr    47 pupae 2d postWPP    45 pupae 3d postWPP    25 pupae 4d postWPP  119 adult male 01day    87 adult male 05day    87 adult male 30day    86 adult female 01day    53 adult female 05day    33 adult female 30day    57 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    1 embryo 08-10hr    2 embryo 10-12hr    7 embryo 12-14hr    9 embryo 14-16hr    12 embryo 16-18hr    13 embryo 18-20hr    44 embryo 20-22hr    49 embryo 22-24hr    34 larva L1    17 larva L2    21 larva L3 12hr old    38 larva L3 puffstage 1-2    35 larva L3 puffstage 3-6    39 larva L3 puffstage 7-9    20 white prepupae new    20 white prepupae 12hr    87 white prepupae 24hr    47 pupae 2d postWPP    45 pupae 3d postWPP    25 pupae 4d postWPP    119 adult male 01day    87 adult male 05day    87 adult male 30day    86 adult female 01day    53 adult female 05day    33 adult female 30day    57 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    0 embryonic S1    1 embryonic S3    1 leg disc CME L1    1 wing disc CME-W2    0 wing disc ML-DmD8    0 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    1 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    1 CNS ML-DmBG1-c1    8 CNS ML-DmBG2-c2    34 tumorous blood cells mbn2    0 ovary fGS/OSS    0 ovary OSC    2 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    0 embryonic S1    1 embryonic S3    1 leg disc CME L1    1 wing disc CME-W2    0 wing disc ML-DmD8    0 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    1 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    1 CNS ML-DmBG1-c1    8 CNS ML-DmBG2-c2  (34) tumorous blood cells mbn2    0 ovary fGS/OSS    0 ovary OSC    2 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    0 embryonic S1    1 embryonic S3    1 leg disc CME L1    1 wing disc CME-W2    0 wing disc ML-DmD8    0 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    1 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    1 CNS ML-DmBG1-c1    8 CNS ML-DmBG2-c2    34 tumorous blood cells mbn2    0 ovary fGS/OSS    0 ovary OSC    2 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    0 embryonic S1    1 embryonic S3    1 leg disc CME L1    1 wing disc CME-W2    0 wing disc ML-DmD8    0 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    1 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    1 CNS ML-DmBG1-c1    8 CNS ML-DmBG2-c2    34 tumorous blood cells mbn2    0 ovary fGS/OSS    0 ovary OSC    2 ovary OSS    0 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    0 embryonic S1    1 embryonic S3    1 leg disc CME L1    1 wing disc CME-W2    0 wing disc ML-DmD8    0 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    1 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    1 CNS ML-DmBG1-c1    8 CNS ML-DmBG2-c2    34 tumorous blood cells mbn2    0 ovary fGS/OSS    0 ovary OSC    2 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    0 embryonic S1    1 embryonic S3    1 leg disc CME L1    1 wing disc CME-W2    0 wing disc ML-DmD8    0 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    1 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    1 CNS ML-DmBG1-c1    8 CNS ML-DmBG2-c2  (34) tumorous blood cells mbn2    0 ovary fGS/OSS    0 ovary OSC    2 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    0 embryonic S1    1 embryonic S3    1 leg disc CME L1    1 wing disc CME-W2    0 wing disc ML-DmD8    0 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    1 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    1 CNS ML-DmBG1-c1    8 CNS ML-DmBG2-c2    34 tumorous blood cells mbn2    0 ovary fGS/OSS    0 ovary OSC    2 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    0 embryonic S1    1 embryonic S3    1 leg disc CME L1    1 wing disc CME-W2    0 wing disc ML-DmD8    0 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    1 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    1 CNS ML-DmBG1-c1    8 CNS ML-DmBG2-c2    34 tumorous blood cells mbn2    0 ovary fGS/OSS    0 ovary OSC    2 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    22 cold shock, 4-day adult    13 heat shock, 4-day adult    21 Cadmium 50 mM 6 hrs, larvae L3    25 Cadmium 50 mM 12 hrs, larvae L3    17 Cadmium 50 mM 48 hrs, 4-day adult    18 Cadmium 100 mM 48 hrs, 4-day adult    18 Copper 0.5 mM 12 hrs, larvae L3    7 Copper 15 mM 48 hrs, 4-day adult    16 Zinc 5 mM 12 hrs, larvae L3    9 Zinc 4.5 mM 48 hrs, 4-day adult    39 Ethanol 2.5% 3 hrs, larvae L3    29 Ethanol 5% 3 hrs, larvae L3    23 Ethanol 10% 3 hrs, larvae L3    24 Caffeine 1.5 mg/ml 4 hrs, larvae L3    33 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    39 Caffeine 25 mg/ml 48 hrs, 4-day adult    18 Paraquat 5 mM 48 hrs, 4-day adult    36 Paraquat 10 mM 48 hrs, 4-day adult    39 Rotenone 2 μg 12 hrs, larvae L3    26 Rotenone 8 μg 12 hrs, larvae L3    29 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    22 cold shock, 4-day adult    13 heat shock, 4-day adult    21 Cadmium 50 mM 6 hrs, larvae L3    25 Cadmium 50 mM 12 hrs, larvae L3    17 Cadmium 50 mM 48 hrs, 4-day adult    18 Cadmium 100 mM 48 hrs, 4-day adult    18 Copper 0.5 mM 12 hrs, larvae L3    7 Copper 15 mM 48 hrs, 4-day adult    16 Zinc 5 mM 12 hrs, larvae L3    9 Zinc 4.5 mM 48 hrs, 4-day adult  (39) Ethanol 2.5% 3 hrs, larvae L3  (29) Ethanol 5% 3 hrs, larvae L3    23 Ethanol 10% 3 hrs, larvae L3    24 Caffeine 1.5 mg/ml 4 hrs, larvae L3  (33) Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (39) Caffeine 25 mg/ml 48 hrs, 4-day adult    18 Paraquat 5 mM 48 hrs, 4-day adult  (36) Paraquat 10 mM 48 hrs, 4-day adult  (39) Rotenone 2 μg 12 hrs, larvae L3  26 Rotenone 8 μg 12 hrs, larvae L3  (29) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    22 cold shock, 4-day adult    13 heat shock, 4-day adult    21 Cadmium 50 mM 6 hrs, larvae L3    25 Cadmium 50 mM 12 hrs, larvae L3    17 Cadmium 50 mM 48 hrs, 4-day adult    18 Cadmium 100 mM 48 hrs, 4-day adult    18 Copper 0.5 mM 12 hrs, larvae L3    7 Copper 15 mM 48 hrs, 4-day adult    16 Zinc 5 mM 12 hrs, larvae L3    9 Zinc 4.5 mM 48 hrs, 4-day adult    39 Ethanol 2.5% 3 hrs, larvae L3    29 Ethanol 5% 3 hrs, larvae L3    23 Ethanol 10% 3 hrs, larvae L3    24 Caffeine 1.5 mg/ml 4 hrs, larvae L3    33 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    39 Caffeine 25 mg/ml 48 hrs, 4-day adult    18 Paraquat 5 mM 48 hrs, 4-day adult    36 Paraquat 10 mM 48 hrs, 4-day adult    39 Rotenone 2 μg 12 hrs, larvae L3    26 Rotenone 8 μg 12 hrs, larvae L3    29 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    22 cold shock, 4-day adult    13 heat shock, 4-day adult    21 Cadmium 50 mM 6 hrs, larvae L3    25 Cadmium 50 mM 12 hrs, larvae L3    17 Cadmium 50 mM 48 hrs, 4-day adult    18 Cadmium 100 mM 48 hrs, 4-day adult    18 Copper 0.5 mM 12 hrs, larvae L3    7 Copper 15 mM 48 hrs, 4-day adult    16 Zinc 5 mM 12 hrs, larvae L3    9 Zinc 4.5 mM 48 hrs, 4-day adult    39 Ethanol 2.5% 3 hrs, larvae L3    29 Ethanol 5% 3 hrs, larvae L3    23 Ethanol 10% 3 hrs, larvae L3    24 Caffeine 1.5 mg/ml 4 hrs, larvae L3    33 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    39 Caffeine 25 mg/ml 48 hrs, 4-day adult    18 Paraquat 5 mM 48 hrs, 4-day adult    36 Paraquat 10 mM 48 hrs, 4-day adult    39 Rotenone 2 μg 12 hrs, larvae L3    26 Rotenone 8 μg 12 hrs, larvae L3    29 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    22 cold shock, 4-day adult    13 heat shock, 4-day adult    21 Cadmium 50 mM 6 hrs, larvae L3    25 Cadmium 50 mM 12 hrs, larvae L3    17 Cadmium 50 mM 48 hrs, 4-day adult    18 Cadmium 100 mM 48 hrs, 4-day adult    18 Copper 0.5 mM 12 hrs, larvae L3    7 Copper 15 mM 48 hrs, 4-day adult    16 Zinc 5 mM 12 hrs, larvae L3    9 Zinc 4.5 mM 48 hrs, 4-day adult    39 Ethanol 2.5% 3 hrs, larvae L3    29 Ethanol 5% 3 hrs, larvae L3    23 Ethanol 10% 3 hrs, larvae L3    24 Caffeine 1.5 mg/ml 4 hrs, larvae L3    33 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    39 Caffeine 25 mg/ml 48 hrs, 4-day adult    18 Paraquat 5 mM 48 hrs, 4-day adult    36 Paraquat 10 mM 48 hrs, 4-day adult    39 Rotenone 2 μg 12 hrs, larvae L3    26 Rotenone 8 μg 12 hrs, larvae L3    29 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    22 cold shock, 4-day adult    13 heat shock, 4-day adult    21 Cadmium 50 mM 6 hrs, larvae L3    25 Cadmium 50 mM 12 hrs, larvae L3    17 Cadmium 50 mM 48 hrs, 4-day adult    18 Cadmium 100 mM 48 hrs, 4-day adult    18 Copper 0.5 mM 12 hrs, larvae L3    7 Copper 15 mM 48 hrs, 4-day adult    16 Zinc 5 mM 12 hrs, larvae L3    9 Zinc 4.5 mM 48 hrs, 4-day adult  (39) Ethanol 2.5% 3 hrs, larvae L3  29 Ethanol 5% 3 hrs, larvae L3    23 Ethanol 10% 3 hrs, larvae L3    24 Caffeine 1.5 mg/ml 4 hrs, larvae L3  (33) Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (39) Caffeine 25 mg/ml 48 hrs, 4-day adult    18 Paraquat 5 mM 48 hrs, 4-day adult  (36) Paraquat 10 mM 48 hrs, 4-day adult  (39) Rotenone 2 μg 12 hrs, larvae L3  26 Rotenone 8 μg 12 hrs, larvae L3  29 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    22 cold shock, 4-day adult    13 heat shock, 4-day adult    21 Cadmium 50 mM 6 hrs, larvae L3    25 Cadmium 50 mM 12 hrs, larvae L3    17 Cadmium 50 mM 48 hrs, 4-day adult    18 Cadmium 100 mM 48 hrs, 4-day adult    18 Copper 0.5 mM 12 hrs, larvae L3    7 Copper 15 mM 48 hrs, 4-day adult    16 Zinc 5 mM 12 hrs, larvae L3    9 Zinc 4.5 mM 48 hrs, 4-day adult    39 Ethanol 2.5% 3 hrs, larvae L3    29 Ethanol 5% 3 hrs, larvae L3    23 Ethanol 10% 3 hrs, larvae L3    24 Caffeine 1.5 mg/ml 4 hrs, larvae L3    33 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    39 Caffeine 25 mg/ml 48 hrs, 4-day adult    18 Paraquat 5 mM 48 hrs, 4-day adult    36 Paraquat 10 mM 48 hrs, 4-day adult    39 Rotenone 2 μg 12 hrs, larvae L3    26 Rotenone 8 μg 12 hrs, larvae L3    29 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    22 cold shock, 4-day adult    13 heat shock, 4-day adult    21 Cadmium 50 mM 6 hrs, larvae L3    25 Cadmium 50 mM 12 hrs, larvae L3    17 Cadmium 50 mM 48 hrs, 4-day adult    18 Cadmium 100 mM 48 hrs, 4-day adult    18 Copper 0.5 mM 12 hrs, larvae L3    7 Copper 15 mM 48 hrs, 4-day adult    16 Zinc 5 mM 12 hrs, larvae L3    9 Zinc 4.5 mM 48 hrs, 4-day adult    39 Ethanol 2.5% 3 hrs, larvae L3    29 Ethanol 5% 3 hrs, larvae L3    23 Ethanol 10% 3 hrs, larvae L3    24 Caffeine 1.5 mg/ml 4 hrs, larvae L3    33 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    39 Caffeine 25 mg/ml 48 hrs, 4-day adult    18 Paraquat 5 mM 48 hrs, 4-day adult    36 Paraquat 10 mM 48 hrs, 4-day adult    39 Rotenone 2 μg 12 hrs, larvae L3    26 Rotenone 8 μg 12 hrs, larvae L3    29 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_10 mE1_20_mRNA_expression_cluster_15
External Data & Images
Linkouts	BDGP expression data • GH10112 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0002930 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG1857-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal necunspecified lethal, with Scer\GAL4pnr-MD237 necGD5273 lethal | adult stage (with nec1) nec2 lethal | adult stage (with nec2) nec1 lethal | recessive nec1 nec2 nec4 nec6 nec7 nec8 nec9 nec10 nec11 nec12 nec13 partially lethal - majority live, with Scer\GAL4Act5C.PI nec1.Scer\UAS nec9.Scer\UAS necScer\UAS.cLa partially lethal - majority live, with Scer\GAL4yolk nec1.Scer\UAS nec9.Scer\UAS necScer\UAS.cLa necSF.Scer\UAS partially lethal - majority live | adult stage | heat sensitive (with nec1) nec2 partially lethal - majority live | adult stage | heat sensitive (with nec2) nec1 nec9 partially lethal - majority live | adult stage | heat sensitive (with nec9) nec2 partially lethal - majority live | heat sensitive, with Scer\GAL4Act5C.PI necSF.Scer\UAS Sterility Allele female sterile (with nec1) nec2 female sterile (with nec2) nec1 Other Phenotypes Allele melanotic necrosis, with Scer\GAL4Act5C.PI necdsRNA.L.Scer\UAS necdsRNA.S.Scer\UAS melanotic necrosis (with nec1) nec2 melanotic necrosis (with nec2) nec1 non-enhancer of variegation, with In(1)wm4 nec1 non-suppressor of variegation, with In(1)wm4 nec1 short lived, with Scer\GAL4Act5C.PI nec1.Scer\UAS nec9.Scer\UAS necScer\UAS.cLa short lived, with Scer\GAL4yolk nec1.Scer\UAS nec9.Scer\UAS necScer\UAS.cLa necSF.Scer\UAS short lived (with nec1) nec2 short lived (with nec2) nec1 short lived | heat sensitive, with Scer\GAL4Act5C.PI necSF.Scer\UAS short lived | heat sensitive (with nec1) nec2 short lived | heat sensitive (with nec2) nec1 nec9 short lived | heat sensitive (with nec9) nec2 visible necunspecified wild-type nec+t7.0 Phenotype manifest in Allele adult abdomen (with nec1) nec2 adult abdomen (with nec2) nec1 adult cuticle (with nec2) nec19 adult cuticle (with nec19) nec2 adult epidermis necunspecified adult epidermis (with nec1) nec2 adult epidermis (with nec2) nec1 adult integumentary system necunspecified joint necunspecified
Classical Alleles ( 22 )
For All Classical Alleles Show
Allele of nec Class Mutagen Stocks Known lesion nec10 amorphic allele - genetic evidence ethyl methanesulfonate 2 Yes nec1 amorphic allele - genetic evidence ethyl methanesulfonate 1 Yes nec2 amorphic allele - genetic evidence ethyl methanesulfonate 1 Yes necJR 1 -- nec11 amorphic allele - genetic evidence ethyl methanesulfonate 0 -- nec12 amorphic allele - genetic evidence ethyl methanesulfonate 0 -- nec13 amorphic allele - genetic evidence ethyl methanesulfonate 0 -- nec4 amorphic allele - genetic evidence ethyl methanesulfonate 0 -- nec6 amorphic allele - genetic evidence ethyl methanesulfonate 0 -- nec7 amorphic allele - genetic evidence ethyl methanesulfonate 0 Yes nec8 amorphic allele - genetic evidence ethyl methanesulfonate 0 -- nec9 amorphic allele - genetic evidence ethyl methanesulfonate 0 Yes nec15 X ray 0 -- nec16 X ray ethyl methanesulfonate 0 Yes nec17 X ray 0 -- nec18 X ray 0 -- nec19 X ray ethyl methanesulfonate 0 Yes nec20 X ray ethyl methanesulfonate 0 Yes nec22 X ray ethyl methanesulfonate 0 Yes necMtnA.T:SV5\V5,T:Zzzz\His6 0 Yes necNpk ethyl methanesulfonate 0 -- necunspecified 0 --
Alleles Carried on Transgenic Constructs ( 14 )
For All Alleles Carried on Transgenic Constructs Show
Allele of nec Class Mutagen Stocks Known lesion necGD5273 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes necKK105206 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes nec+t7.0 in vitro construct - genomic fragment 0 Yes nec1.Scer\UAS in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes nec9.Scer\UAS in vitro construct - regulatory fusion in vitro construct - site directed mutagenesis 0 Yes necdsRNA.57-1473 in vitro construct - RNAi 0 Yes necdsRNA.CopT.Scer\UAS in vitro construct - regulatory fusion in vitro construct - RNAi 0 Yes necdsRNA.L.Scer\UAS in vitro construct - regulatory fusion in vitro construct - RNAi 0 Yes necdsRNA.S.Scer\UAS in vitro construct - regulatory fusion in vitro construct - RNAi 0 Yes necK68Q.K70Q.K71Q.Scer\UAS in vitro construct - regulatory fusion 0 Yes necNIG.1857R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes necPP.Scer\UAS in vitro construct - regulatory fusion in vitro construct - amino acid replacement 0 Yes necScer\UAS.cLa in vitro construct - regulatory fusion 0 Yes necSF.Scer\UAS in vitro construct - regulatory fusion in vitro construct - site directed mutagenesis 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2R)cn-S5 (Heitzler et al., 1993) Df(2R)Drlrv3 (Heitzler et al., 1993) Df(2R)Drlrv5 (Heitzler et al., 1993) Df(2R)Drlrv7 (Heitzler et al., 1993) Df(2R)Drlrv15 (Heitzler et al., 1993) Df(2R)Drlrv17 (Heitzler et al., 1993) Df(2R)Drlrv18 (Heitzler et al., 1993) Df(2R)Drlrv20 (Heitzler et al., 1993) Df(2R)Drlrv24 (Heitzler et al., 1993) Df(2R)Drlrv25 (Heitzler et al., 1993) Df(2R)Drlrv26 (Heitzler et al., 1993) Df(2R)Drlrv28 (Heitzler et al., 1993) Df(2R)nap2 (Levashina et al., 1999, Green et al., 2000, Heitzler et al., 1993) Df(2R)Npk-rv1 (Roote and Ashburner, 2005.6) Df(2R)Npk-rv2 (Roote and Ashburner, 2005.6) Df(2R)Npk-rv4 (Roote and Ashburner, 2005.6) Df(2R)Npk-rv5 (Roote and Ashburner, 2005.6) Df(2R)Npk-rv6 (Roote and Ashburner, 2005.6) Df(2R)Npk-rv7 (Roote and Ashburner, 2005.6) Df(2R)pk78k (Green et al., 2000) Df(2R)pk-N5 (Heitzler et al., 1993) Df(2R)pk-sple-50 (Gubb et al., 1999) Df(2R)pk-sple-51 (Gubb et al., 1999) Df(2R)sple-D1 (Green et al., 2000, Heitzler et al., 1993) Df(2R)sple-D2 (Green et al., 2000, Heitzler et al., 1993) Df(2R)sple-D3 (Heitzler et al., 1993, Roote, 1996.8) Df(2R)sple-J1 (Levashina et al., 1999, Heitzler et al., 1993) Df(2R)sple-J2 (Heitzler et al., 1993) Df(2R)sple-N3 (Heitzler et al., 1993) In(2LR)pk44 (Gubb et al., 1999) In(2LR)TE35B-14LScorv1R (Roote et al., 1996, Roote, 1996.8) In(2LR)TE35B-14LTE35B-4R (Roote, 1996.8, Roote, 1997.6.6) In(2R)nec-5 (Gubb et al., 1999) In(2R)nec-e3 (Gubb et al., 1999) T(2;3)pk-sple31 (Gubb et al., 1999) T(Y;2)pk-sple45 (Gubb et al., 1999) Tp(2;2)pk-sple22 (Gubb et al., 1999) Tp(2;2)pk-sple24 (Gubb et al., 1999)
Not disrupted in	Df(2R)cn-S3 (Heitzler et al., 1993) Df(2R)cn-S6 (Heitzler et al., 1993) Df(2R)cn-S8 (Heitzler et al., 1993) Df(2R)cos-2 (Heitzler et al., 1993) Df(2R)cos-3 (Heitzler et al., 1993) Df(2R)Drlrv21 (Heitzler et al., 1993) Df(2R)Drlrv22 (Heitzler et al., 1993) Df(2R)Drlrv30 (Heitzler et al., 1993) Df(2R)DX1 (Heitzler et al., 1993) Df(2R)DX14 (Heitzler et al., 1993) Df(2R)EW60 (Heitzler et al., 1993) Df(2R)H23 (Heitzler et al., 1993) Df(2R)NCX5 (Heitzler et al., 1993) Df(2R)NCX8 (Heitzler et al., 1993) Df(2R)NCX9 (Heitzler et al., 1993) Df(2R)NCX10 (Heitzler et al., 1993) Df(2R)NCX11 (Heitzler et al., 1993) Df(2R)NCX13 (Heitzler et al., 1993) Df(2R)Npk-rv3 (Roote and Ashburner, 2005.6) Df(2R)pwn5 (Heitzler et al., 1993) Df(2R)tor-rx6 (Heitzler et al., 1993)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD5273} NA P{KK105206} NA P{NIG.1857R} NA P{UAS-nec.1} NA P{UAS-nec.9} NA P{UAS-nec.K68Q.K70Q.K71Q} NA P{UAS-nec.L} NA P{UAS-nec.L-dsRNA} NA P{UAS-nec.S-dsRNA} NA P{UAS-nec.SF} NA P{UAS-necPP.G} NA P{UAS-nec-CopT-dsRNA} NA characterization construct P{nec.7.0.G} NA P{UAS-f::nec-dsRNA} NA
Insertions
	Type of insertions Name Expression data
Gene Ontology: Function, Process & Cellular Component ( 9 unique terms )
Terms Based on Experimental Evidence ( 6 terms )
Molecular Function
CV term Evidence References
serine-type endopeptidase inhibitor activity inferred from direct assay (Robertson et al., 2003)
Biological Process
CV term Evidence References
antifungal humoral response inferred from genetic interaction with spz AND traceable author statement (Green et al., 2000) immune response inferred from expression pattern (Levashina et al., 1999) negative regulation of Toll signaling pathway inferred from mutant phenotype (Levashina et al., 1999) regulation of proteolysis inferred from direct assay (Robertson et al., 2003) inferred from mutant phenotype (Levashina et al., 1999)
Cellular Component
CV term Evidence References
extracellular space inferred from direct assay (Levashina et al., 1999)
Terms Based on Predictions or Assertions ( 4 terms )
Molecular Function
CV term Evidence References
serine-type endopeptidase inhibitor activity inferred from sequence or structural similarity with EMBL:U58361 (FlyBase, 1992-) non-traceable author statement (Levashina et al., 1998, Leclerc and Reichhart, 2004)
Biological Process
CV term Evidence References
defense response non-traceable author statement (Lemaitre, 2000.12.20) negative regulation of endopeptidase activity inferred from biological aspect of ancestor with PANTHER:PTN000156122 (assigned by RefGenome) (Gaudet et al., 2010) Toll signaling pathway non-traceable author statement (Lemaitre, 2000.12.20) traceable author statement (Kimbrell and Beutler, 2001, Carrell and Corral, 2004)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions nec allele Gene References nec1 psh (Ligoxygakis et al., 2002) spz (Green et al., 2000) nec2 psh (Ligoxygakis et al., 2002) spz (Green et al., 2000)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 69974 DroID - A comprehensive database of gene and protein interactions. • FBgn0002930 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 45908
Orthologs
OrthoDB Orthologs (283) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG63N7ZJ Dipteran inclusive ortholog search No orthologs identified Insect inclusive ortholog search EOG6DV4HB Arthropod inclusive ortholog search EOG6XWDDG Metazoa inclusive ortholog search EOG6TXQN0
Orthologs in Drosophila Species (EOG63N7ZJ)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\nec     Drosophila simulans   Dsim\nec Y   Drosophila sechellia   Dsec\GM20812 Y   Drosophila erecta   Dere\GG10764 Y   Drosophila yakuba   Dyak\nec Y   Drosophila ananassae   Dana\GF12243 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA14995 Y   Drosophila persimilis   Dper\GL10947 Y   Drosophila willistoni   Dwil\GK21526 Y   Drosophila virilis   Dvir\GJ14938 Y   Drosophila mojavensis   Dmoj\GI20049 Y   Drosophila grimshawi   Dgri\GH20376 Y
Orthologs in non-Drosophila Dipterans (None identified)
No non-Drosophilid orthologies identified
Orthologs in non-Dipteran Insects (EOG6DV4HB)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB46970 Y Apis mellifera Western honey bee  Amel\GB48820 Y Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG007826 Y Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG007817 Y Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG004051 Y Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG007825 Y Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG007190 Y Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH19678 Y Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH15231 Y Atta cephalotes Leafcutter ant  Acep\ACEP13675 Y Camponotus floridanus Florida carpenter ant  Cflo\CFLO20012 Y Camponotus floridanus Florida carpenter ant  Cflo\CFLO12281 Y Camponotus floridanus Florida carpenter ant  Cflo\CFLO18070 Y Camponotus floridanus Florida carpenter ant  Cflo\CFLO26126 Y Camponotus floridanus Florida carpenter ant  Cflo\CFLO20846 Y Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL20634 Y Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL19553 Y Linepithema humile Argentine ant  Lhum\LH24668 Y Linepithema humile Argentine ant  Lhum\LH26242 Y Pogonomyrmex barbatus Red harvester ant  Pbar\PB23525 Y Pogonomyrmex barbatus Red harvester ant  Pbar\PB24320 Y Solenopsis invicta Red fire ant  Sinv\SINV14300 Y Solenopsis invicta Red fire ant  Sinv\SINV12644 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI000344 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI005016 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI006911 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI004935 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI004123 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI008381 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI086194 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI060557 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI002244 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI008775 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI007530 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI080561 Y Bombyx mori Silkmoth  Bmor\BGIBMGA007720 Y Bombyx mori Silkmoth  Bmor\BGIBMGA009953 Y Pediculus humanus Human body louse  Phum\PHUM220550 Y Tribolium castaneum Red flour beetle  Tcas\TC005747 Y Tribolium castaneum Red flour beetle  Tcas\serpin23 Y Tribolium castaneum Red flour beetle  Tcas\serpin29 Y Tribolium castaneum Red flour beetle  Tcas\serpin17 Y Tribolium castaneum Red flour beetle  Tcas\serpin25 Y Tribolium castaneum Red flour beetle  Tcas\serpin21 Y Tribolium castaneum Red flour beetle  Tcas\serpin20 Y Tribolium castaneum Red flour beetle  Tcas\serpin7 Y Tribolium castaneum Red flour beetle  Tcas\serpin18 Y Tribolium castaneum Red flour beetle  Tcas\TC005754 Y Tribolium castaneum Red flour beetle  Tcas\serpin19 Y Tribolium castaneum Red flour beetle  Tcas\TC005746 Y Tribolium castaneum Red flour beetle  Tcas\serpin12 Y Tribolium castaneum Red flour beetle  Tcas\serpin3 Y Tribolium castaneum Red flour beetle  Tcas\serpin24 Y Tribolium castaneum Red flour beetle  Tcas\serpin1 Y Tribolium castaneum Red flour beetle  Tcas\serpin31 Y
Orthologs in non-Insect Arthropods (EOG6XWDDG)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\Csda Y Daphnia pulex Water flea  Dpux\RHBG Y Daphnia pulex Water flea  Dpux\cyp-33d3 Y Ixodes scapularis Deer tick  Isca\ISCW018607 Y Ixodes scapularis Deer tick  Isca\ISCW011013 Y Ixodes scapularis Deer tick  Isca\ISCW014779 Y Ixodes scapularis Deer tick  Isca\ISCW005088 Y Ixodes scapularis Deer tick  Isca\ISCW023623 Y Ixodes scapularis Deer tick  Isca\ISCW024342 Y Ixodes scapularis Deer tick  Isca\ISCW000427 Y Ixodes scapularis Deer tick  Isca\ISCW014652 Y Ixodes scapularis Deer tick  Isca\ISCW023624 Y Ixodes scapularis Deer tick  Isca\ISCW009616 Y Ixodes scapularis Deer tick  Isca\ISCW004156 Y Ixodes scapularis Deer tick  Isca\ISCW023618 Y Ixodes scapularis Deer tick  Isca\ISCW023620 Y Ixodes scapularis Deer tick  Isca\ISCW024109 Y Ixodes scapularis Deer tick  Isca\ISCW024046 Y Ixodes scapularis Deer tick  Isca\ISCW024387 Y Ixodes scapularis Deer tick  Isca\ISCW024435 Y Ixodes scapularis Deer tick  Isca\ISCW015198 Y Ixodes scapularis Deer tick  Isca\ISCW024495 Y Ixodes scapularis Deer tick  Isca\ISCW024500 Y Ixodes scapularis Deer tick  Isca\ISCW011017 Y Ixodes scapularis Deer tick  Isca\ISCW023619 Y Ixodes scapularis Deer tick  Isca\ISCW023617 Y Ixodes scapularis Deer tick  Isca\ISCW024013 Y Ixodes scapularis Deer tick  Isca\ISCW023622 Y Ixodes scapularis Deer tick  Isca\ISCW023621 Y Ixodes scapularis Deer tick  Isca\ISCW015204 Y
Orthologs in non-Arthropod Metazoa (EOG6TXQN0)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\srp-7 Y Caenorhabditis elegans Nematode  Cele\srp-1 Y Caenorhabditis elegans Nematode  Cele\srp-8 Y Caenorhabditis elegans Nematode  Cele\srp-6 Y Caenorhabditis elegans Nematode  Cele\srp-2 Y Caenorhabditis elegans Nematode  Cele\srp-3 Y Caenorhabditis elegans Nematode  Cele\srp-5 Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_013378gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_018631gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_018630gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_018196gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_013377gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_020278gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_002711gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_024263gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_028469gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_004543gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_009346gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_018632gn Y Danio rerio Zebrafish  Drer\ENSDARG00000090916 Y Danio rerio Zebrafish  Drer\serpind1 Y Danio rerio Zebrafish  Drer\zgc:174259 Y Danio rerio Zebrafish  Drer\zgc:173729 Y Danio rerio Zebrafish  Drer\serpinb1l4 Y Danio rerio Zebrafish  Drer\ENSDARG00000091800 Y Danio rerio Zebrafish  Drer\serpinb1l1 Y Danio rerio Zebrafish  Drer\serpine2 Y Danio rerio Zebrafish  Drer\serpine3 Y Danio rerio Zebrafish  Drer\serpinb1l3 Y Danio rerio Zebrafish  Drer\serpinb1l2 Y Danio rerio Zebrafish  Drer\serpina1 Y Danio rerio Zebrafish  Drer\serpinc1 Y Danio rerio Zebrafish  Drer\serpine1 Y Danio rerio Zebrafish  Drer\serpini1 Y Danio rerio Zebrafish  Drer\ENSDARG00000036968 Y Danio rerio Zebrafish  Drer\serpina1l Y Danio rerio Zebrafish  Drer\si:ch73-303g23.4 Y Danio rerio Zebrafish  Drer\serpinb1 Y Danio rerio Zebrafish  Drer\si:ch211-186e20.2 Y Xenopus tropicalis Western clawed frog  Xtro\serpinc1 Y Xenopus tropicalis Western clawed frog  Xtro\serpinb4 Y Xenopus tropicalis Western clawed frog  Xtro\serpinb6 Y Xenopus tropicalis Western clawed frog  Xtro\serpina3k-prov Y Xenopus tropicalis Western clawed frog  Xtro\ENSXETG00000009423 Y Xenopus tropicalis Western clawed frog  Xtro\ENSXETG00000032417 Y Xenopus tropicalis Western clawed frog  Xtro\ENSXETG00000032542 Y Xenopus tropicalis Western clawed frog  Xtro\serpinb1 Y Xenopus tropicalis Western clawed frog  Xtro\serpini1 Y Xenopus tropicalis Western clawed frog  Xtro\serpinb11 Y Xenopus tropicalis Western clawed frog  Xtro\serpine1 Y Xenopus tropicalis Western clawed frog  Xtro\serpina3 Y Xenopus tropicalis Western clawed frog  Xtro\serpini2 Y Xenopus tropicalis Western clawed frog  Xtro\serpina3m Y Xenopus tropicalis Western clawed frog  Xtro\serpina1 Y Xenopus tropicalis Western clawed frog  Xtro\serpine2 Y Xenopus tropicalis Western clawed frog  Xtro\serpinb5 Y Xenopus tropicalis Western clawed frog  Xtro\serpind1 Y Gallus gallus Domestic chicken  Ggal\SERPINE2 Y Gallus gallus Domestic chicken  Ggal\SERPINB14B Y Gallus gallus Domestic chicken  Ggal\SERPINC1 Y Gallus gallus Domestic chicken  Ggal\SERPINB10 Y Gallus gallus Domestic chicken  Ggal\SERPINB1 Y Gallus gallus Domestic chicken  Ggal\SERPINE3 Y Gallus gallus Domestic chicken  Ggal\SERPINB12 Y Gallus gallus Domestic chicken  Ggal\SERPINBP1 Y Gallus gallus Domestic chicken  Ggal\SERPINB5 Y Gallus gallus Domestic chicken  Ggal\SERPINB14 Y Gallus gallus Domestic chicken  Ggal\SERPINA3 Y Gallus gallus Domestic chicken  Ggal\SERPINB10 Y Gallus gallus Domestic chicken  Ggal\SERPINA4 Y Gallus gallus Domestic chicken  Ggal\SERPIND1 Y Gallus gallus Domestic chicken  Ggal\SERPINI1 Y Gallus gallus Domestic chicken  Ggal\SERPINA9 Y Gallus gallus Domestic chicken  Ggal\SERPINA5 Y Gallus gallus Domestic chicken  Ggal\SERPINB2 Y Gallus gallus Domestic chicken  Ggal\SERPINA1 Y Gallus gallus Domestic chicken  Ggal\SERPINB6 Y Mus musculus House mouse  Mmus\Serpinb9e Y Mus musculus House mouse  Mmus\Serpinb3d Y Mus musculus House mouse  Mmus\ENSMUSG00000069248 Y Mus musculus House mouse  Mmus\Serpina9 Y Mus musculus House mouse  Mmus\Serpina1b Y Mus musculus House mouse  Mmus\Serpine3 Y Mus musculus House mouse  Mmus\Serpina3f Y Mus musculus House mouse  Mmus\Serpinb9b Y Mus musculus House mouse  Mmus\Serpinb9f Y Mus musculus House mouse  Mmus\Serpina5 Y Mus musculus House mouse  Mmus\Serpina11 Y Mus musculus House mouse  Mmus\Serpina3m Y Mus musculus House mouse  Mmus\Serpine1 Y Mus musculus House mouse  Mmus\Serpinb1c Y Mus musculus House mouse  Mmus\Serpinb1b Y Mus musculus House mouse  Mmus\Serpina3i Y Mus musculus House mouse  Mmus\Serpinb6d Y Mus musculus House mouse  Mmus\Serpina6 Y Mus musculus House mouse  Mmus\Serpinb3a Y Mus musculus House mouse  Mmus\Serpind1 Y Mus musculus House mouse  Mmus\Serpinb9 Y Mus musculus House mouse  Mmus\Serpine2 Y Mus musculus House mouse  Mmus\Serpinb9d Y Mus musculus House mouse  Mmus\Serpinb5 Y Mus musculus House mouse  Mmus\ENSMUSG00000042842 Y Mus musculus House mouse  Mmus\Serpina1d Y Mus musculus House mouse  Mmus\Serpinb11 Y Mus musculus House mouse  Mmus\Serpina1a Y Mus musculus House mouse  Mmus\Serpinb3c Y Mus musculus House mouse  Mmus\Serpinb3b Y Mus musculus House mouse  Mmus\Serpina12 Y Mus musculus House mouse  Mmus\Serpinc1 Y Mus musculus House mouse  Mmus\ENSMUSG00000044734 Y Mus musculus House mouse  Mmus\Serpina1e Y Mus musculus House mouse  Mmus\Serpina3c Y Mus musculus House mouse  Mmus\Serpina3n Y Mus musculus House mouse  Mmus\Serpina3a Y Mus musculus House mouse  Mmus\Serpini1 Y Mus musculus House mouse  Mmus\Serpinb6c Y Mus musculus House mouse  Mmus\ENSMUSG00000048775 Y Mus musculus House mouse  Mmus\Serpinb8 Y Mus musculus House mouse  Mmus\Serpina3j Y Mus musculus House mouse  Mmus\ENSMUSG00000021404 Y Mus musculus House mouse  Mmus\Serpinb7 Y Mus musculus House mouse  Mmus\Serpini2 Y Mus musculus House mouse  Mmus\Serpinb6a Y Mus musculus House mouse  Mmus\ENSMUSG00000079015 Y Rattus norvegicus Norway rat  Rnor\Serpinb13 Y Rattus norvegicus Norway rat  Rnor\Serpinb6b Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000029949 Y Rattus norvegicus Norway rat  Rnor\Serpine1 Y Rattus norvegicus Norway rat  Rnor\Serpinb1b Y Rattus norvegicus Norway rat  Rnor\Serpini2 Y Rattus norvegicus Norway rat  Rnor\Serpina4 Y Rattus norvegicus Norway rat  Rnor\Serpinb10 Y Rattus norvegicus Norway rat  Rnor\Serpina9 Y Rattus norvegicus Norway rat  Rnor\Serpinb7 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000011141 Y Rattus norvegicus Norway rat  Rnor\Serpina7 Y Rattus norvegicus Norway rat  Rnor\Serpini1 Y Rattus norvegicus Norway rat  Rnor\Serpinb3a Y Rattus norvegicus Norway rat  Rnor\Serpind1 Y Rattus norvegicus Norway rat  Rnor\Serpinb6a Y Rattus norvegicus Norway rat  Rnor\Serpinb1a Y Rattus norvegicus Norway rat  Rnor\LOC299282 Y Rattus norvegicus Norway rat  Rnor\Serpinb5 Y Rattus norvegicus Norway rat  Rnor\Serpinc1 Y Rattus norvegicus Norway rat  Rnor\Serpina12 Y Rattus norvegicus Norway rat  Rnor\Serpinb2 Y Rattus norvegicus Norway rat  Rnor\LOC299277 Y Rattus norvegicus Norway rat  Rnor\Serpine2 Y Rattus norvegicus Norway rat  Rnor\Serpinb11 Y Rattus norvegicus Norway rat  Rnor\Serpinb9 Y Rattus norvegicus Norway rat  Rnor\Serpina6 Y Rattus norvegicus Norway rat  Rnor\Serpine3 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000032669 Y Rattus norvegicus Norway rat  Rnor\Serpina5 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000010410 Y Rattus norvegicus Norway rat  Rnor\Serpinb12 Y Rattus norvegicus Norway rat  Rnor\Serpinb9e Y Rattus norvegicus Norway rat  Rnor\Serpinb8 Y Rattus norvegicus Norway rat  Rnor\Serpina3n Y Rattus norvegicus Norway rat  Rnor\Serpinb3 Y Rattus norvegicus Norway rat  Rnor\Serpinb6e Y Homo sapiens Human  Hsap\SERPINB2 Y Homo sapiens Human  Hsap\SERPINC1 Y Homo sapiens Human  Hsap\SERPINA3 Y Homo sapiens Human  Hsap\SERPINB9 Y Homo sapiens Human  Hsap\SERPINA4 Y Homo sapiens Human  Hsap\SERPINI2 Y Homo sapiens Human  Hsap\SERPIND1 Y Homo sapiens Human  Hsap\ENSG00000170054 Y Homo sapiens Human  Hsap\SERPINI1 Y Homo sapiens Human  Hsap\SERPINB8 Y Homo sapiens Human  Hsap\ENSG00000135919 Y Homo sapiens Human  Hsap\SERPINE3 Y Homo sapiens Human  Hsap\SERPINB5 Y Homo sapiens Human  Hsap\SERPINA12 Y Homo sapiens Human  Hsap\SERPINA7 Y Homo sapiens Human  Hsap\ENSG00000242550 Y Homo sapiens Human  Hsap\SERPINA11 Y Homo sapiens Human  Hsap\SERPINB4 Y Homo sapiens Human  Hsap\SERPINB1 Y Homo sapiens Human  Hsap\SERPINA6 Y Homo sapiens Human  Hsap\SERPINB12 Y Homo sapiens Human  Hsap\SERPINA1 Y Homo sapiens Human  Hsap\SERPINB3 Y Homo sapiens Human  Hsap\SERPINA5 Y Homo sapiens Human  Hsap\SERPINE1 Y
Human Orthologs (25)
Gene OMIM HGNC Hsap\SERPINB2     Hsap\SERPINC1     Hsap\SERPINA3     Hsap\SERPINB9     Hsap\SERPINA4     Hsap\SERPINI2     Hsap\SERPIND1     Hsap\ENSG00000170054     Hsap\SERPINI1     Hsap\SERPINB8     Hsap\ENSG00000135919     Hsap\SERPINE3     Hsap\SERPINB5     Hsap\SERPINA12     Hsap\SERPINA7     Hsap\ENSG00000242550     Hsap\SERPINA11     Hsap\SERPINB4     Hsap\SERPINB1     Hsap\SERPINA6     Hsap\SERPINB12     Hsap\SERPINA1     Hsap\SERPINB3     Hsap\SERPINA5     Hsap\SERPINE1
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\nec Drosophila sechellia Dsec\GM20812 Drosophila erecta Dere\GG10764 Drosophila yakuba Dyak\nec Drosophila ananassae Dana\GF12243 Drosophila pseudoobscura pseudoobscura Dpse\GA14995 Drosophila persimilis Dper\GL10947 Drosophila willistoni Dwil\GK21526 Drosophila virilis Dvir\GJ14938 Drosophila mojavensis Dmoj\GI20049 Drosophila grimshawi Dgri\GH20376
Stocks & Reagents
Stocks Listed in FlyBase ( 8 )
Bloomington	9151 Dp(?;2)bwD, nec2 bwD/CyO 4288 nec10 cn1 bw1 sp1/CyO 11382 P{PZ}l(2)43Bb04614a cn1/CyO, necJR; ry506 P{PZ}stg04614b
Kyoto	107672 nec10 cn1 bw1 sp1/CyO 101403 nec1 bwD / CyO, Tp(2;2)pk-sple26
VDRC	v108366 P{KK105206}VIE-260B v37958 w1118; P{GD5273}v37958 v44837 w1118; P{GD5273}v44837
Genomic Clones ( 1 )
	BACR12F04 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 33 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GH10112
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT26992 GH01054 GH07566 GH17392 GH17608 GH18542 GH21227 GH22377 GH22817 GH23919 GH26737 GH26837 HL02838 HL04407 HL07334 LP16986 LP17213
Other clones	44021 59496 60457 101917 161390 214435.40 Dmel_dig_000401_0627_0323 EC22258 EC054735 EK160259 EK184358 EK228811 EK265838 EP05241 ESG0110c.E21_E09
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0002930 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 45908
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: nec anon-WO0118057.3 (Gubb, 1998.3.20, FlyBase, 1996-) Source for merge of: nec CG1857 (Lemaitre, 2000.9.1)
Additional comments	Source for merge of nec anon-WO0118057.3 was sequence comparison (date:030912). (Gubb, 1998.3.20, FlyBase, 1996-)
Other Comments
	nec requires spe, sphinx2, sphinx1, spheroide, grass, and spirit to activate Tl. (Kambris et al., 2006) The Tl-mediated immune response to fungal infections is constitutively activated in nec mutants and pleiotropic phenotypes include melanization and cellular necrosis. (Green et al., 2000) nec negatively regulates the Tl signalling pathway. (Levashina et al., 1999) nec has a role in Tl mediated antifungal defence. (Levashina et al., 1999) Deletion mutants for all three serpins (Spn43Aa, Spn43Ab and nec) survive as adults for a short while before dying with an extreme nec phenotype. (Gubb, 1998.3.20) The cellular polarity gene pk and the adult lethal nec gene map within the proximal region of pk-sple complex among a set of 3 Serpin transcripts. (Gubb and Green, 1995)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_080112 RefSeq (Proteins) • NP_524851 DDBJ / EMBL / GenBank • AAF59285 AAM27488 AAQ64949 AAQ64950 AAQ64951 AAQ64952 AAQ64953 AAQ64954 AAQ64955 AAQ64956 AAQ64957 AAQ64958 AI113685 AI402196 AJ245444 AX100272 AY102659 AY349666 AY349667 AY349668 AY349669 AY349670 AY349671 AY349672 AY349673 AY349674 AY349675 CAB56697 Entrez Gene - A searchable database of RefSeq genes. • 45908 UniProtKB/TrEMBL • Q6V6U1 Q6V6U2 Q6V6U3 Q6V6U4 Q6V6U5 Q6V6U6 Q6V6U7 Q6V6U8 Q6V6U9 Q6V6V0 Q7JWX3 Q9U5W7
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Serpin family (IPR000215) Serpin domain (IPR023796) Protease inhibitor I4, serpin, conserved site (IPR023795) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • GH10112
Linkouts
	BioGRID - A database of protein and genetic interactions • 69974 DroID - A comprehensive database of gene and protein interactions. • FBgn0002930 DRSC - Results from RNAi screens. • FBgn0002930 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0002930 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG1857-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0002930 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 45908 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 45908 modMine - Data generated by the modENCODE project. • FBgn0002930
Synonyms & Secondary IDs ( 13 )
Reported As
Symbol Synonym	anon-WO0118057.3   CG1857 (Irving et al., 2001, De Gregorio et al., 2001, Lemaitre, 2000.9.1, Pal et al., 2007, Ellis and Carney, 2009, Carrera et al., 2008, Keleman et al., 2009.8.5, Easow et al., 2007, Hernandez-Romano et al., 2008, Garrett et al., 2009, Wertheim et al., 2005, Wong et al., 2012) nec (Kambris et al., 2006, Lee et al., 2007, Kambris et al., 2006, Blanco et al., 2010, Lee et al., 2007, Robertson et al., 2006, Christophides et al., 2002, Hill-Burns and Clark, 2010, Carrera et al., 2008, Soukup et al., 2009, Scherfer et al., 2008, Wertheim et al., 2005, Yan et al., 2010, Japanese National Institute of Genetics, 2012.5.21, Hashimoto et al., 2012.10.8) Nec (Pal et al., 2007, Shin et al., 2005, Soukup and Gubb, 2007, Waterhouse et al., 2007, Pelte et al., 2006, Soukup and Gubb, 2008, Busse et al., 2007, Ahmad et al., 2009, Obbard et al., 2009, Avet-Rochex et al., 2010) NEC (Matskevich et al., 2010) SER3 (Reichhart et al., 1998) Ser3 (Levashina et al., 1998) Spn43Ac (Goto et al., 2003, De Gregorio et al., 2002, Kimbrell and Beutler, 2001, Imler and Hoffmann, 2001, Silverman et al., 2001, Gubb et al., 2001, Gubb, 1999.7.27, Green et al., 2000, Barillas-Mury et al., 2000, Jiang and Kanost, 2000, Levashina et al., 1999, Gubb et al., 1999, Tang et al., 2007, Ahmad et al., 2009, Garrett et al., 2009, Reichhart et al., 2011)
Name Synonym	necrotic (Pal et al., 2007, Soukup and Gubb, 2008, Zhou et al., 2008, Buchon et al., 2009, Crowther et al., 2005, Luheshi et al., 2010, Wong et al., 2012, Hashimoto et al., 2012.10.8) Necrotic (Lee et al., 2007, Robertson et al., 2006, Roxstrom-Lindquist et al., 2004, Ahmad et al., 2009, Hernandez-Romano et al., 2008, Zsindely et al., 2009) necrotic serpin (Tang et al., 2007, Matskevich et al., 2010) Serine protease inhibitor 3 (Reichhart et al., 1998) serpin43Ac (Lemaitre, 2000.12.20)
Secondary FlyBase IDs
	FBgn0024292 FBgn0028676 FBgn0033148 FBgn0063807
References ( 106 )
Generate a list of	All references relating to this gene ( 106 )
List References by type	Research paper  ( 47 ) Supplementary material  ( 4 ) Review  ( 19 ) Personal communication to FlyBase  ( 13 ) Abstract  ( 14 ) FlyBase analysis  ( 2 ) DNA/RNA sequence record  ( 1 ) Stock list  ( 1 ) Website  ( 1 ) Letter  ( 1 ) Curated genome annotation  ( 2 ) Patent  ( 1 )
Recent research papers ( 2 )
	Wong et al., 2012, Mol. Biol. Evol. 29(1): 229--238 Temporally variable selection on proteolysis-related reproductive tract proteins in Drosophila. [FBrf0217443] Baycin-Hizal et al., 2011, J. Proteome Res. 10(6): 2777--2784 GlycoFly: A Database of Drosophila N-linked Glycoproteins Identified Using SPEG-MS Techniques. [FBrf0213834] Show all research papers ...
Recent reviews ( 1 )
	Reichhart et al., 2011, Methods Enzymol. 499: 205--225 The Drosophila serpins multiple functions in immunity and morphogenesis. [FBrf0213937] Show all reviews ...