• Home
• Tools
  • Tools Overview
  • Query Tools

    
    • QuickSearch
    • QueryBuilder
    • TermLink
    • CytoSearch
    • Interactions Browser
    • ImageBrowse
    • Find A Person
    • Google™ FlyBase
  • Genomic/Map Tools

    
    • BLAST
    • GBrowse
    • CytoSearch
    • Aberrations Maps
    • Chromosome Maps
    • Coordinates Converter
  • Retrieve/Convert

    
    • Batch Download
    • Coordinates Converter
    • ID Converter
  • Data Submission

    
    • Fast-Track Your Paper
    • Submit Personal Comm
    • Add A New Person
    • Update Person Info
  • People

    
    • Find A Person
    • Add A New Person
    • Update Person Info
• Files
  • Files Overview
  • Downloads:
  • Precomputed files
  • Archived data
  • Releases (FTP)
  • Genomes (FTP)
  • AAA data (FTP)
  • Lists:
  • Transposons (Dmel)
  • Transposons (other)
  • Vectors & Constructs
  • Anatomical Terms
  • Map Conversion
• Species
  • Phylogeny
  • Sequenced Species
  • Synteny Table
  • Color Illustrations
  • Abbreviations
• Documents
  • About FlyBase
  • Reference Manual:
  • Sections
  • Contents
  • Nomenclature
  • Release Notes
• Resources
  • All Resources
  • Model Organisms
  • Stock Collections
  • Other links:
  • BDGP
  • DGRC
  • FlyExpress
  • Interactive Fly
  • modENCODE
  • Textpresso for Fly
• News
  • News
  • FlyBase Forum
  • FlyBase Positions
  • Meetings
  • Courses
  • Fly Board
  • White papers
  • Funding
  • bionet.dros
• Help
  • Google™ FlyBase
  • Site Map
  • FAQs
  • FlyBase Guides
  • Video Tutorials
  • Report help
  • New to Flies
  • Pers. comm.
  • Author guidelines
  • Contact FlyBase
• Archives
  • Prior Release
  • Other Archives
  • Coordinate Converter

Gene Dmel\nec

General Information
Symbol	Dmel\nec	Species	D. melanogaster
Name	necrotic	Annotation symbol	CG1857
Feature type	protein_coding_gene	FlyBase ID	FBgn0002930
Gene Model Status	Current	Stock availability	8 publicly available
Also Known As	Spn43Ac, SER3
Genomic Location
Chromosome (arm)	2R	Recombination map
Cytogenetic map	42F3-42F3	Sequence location	2R:3,043,981..3,045,733 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene necrotic is referred to in FlyBase by the symbol Dmel\nec (CG1857, FBgn0002930). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: serine-type endopeptidase inhibitor activity. There is experimental evidence that it is involved in the biological process: immune response; negative regulation of Toll signaling pathway; antifungal humoral response; regulation of proteolysis. 35 alleles are reported. The phenotypes of these alleles are annotated with: adult epidermis; adult cuticle; adult integumentary system; adult abdomen; joint. It has one annotated transcript and one annotated polypeptide. Protein features are: Protease inhibitor I4, serpin; Protease inhibitor I4, serpin, conserved site; Serpin domain. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of extremely low expression. Peak expression observed at stages throughout the pupal period, in stages of adults of both sexes. Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, adult crop, adult heart, larval/adult fat body, adult spermathecae, adult carcass. Expression at moderate levels in the following post-embryonic organs or tissues: adult central nervous system, larval/adult hindgut. Comments on Affy2 ProbeSet: ProbeSet 1636653_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of nec. Gene sequence location is 2R:3043981..3045733.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2011_10
FB2012_01	Sequence features BKN24342 HFA06769
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 42F3-42F3   Limits computationally determined from genome sequence between P{lacW}vimark16722 and P{lacW}cosk16101
Experimentally Determined Cytological Location
Cytogenetic map Notes References 43A1-43A2   (determined by in situ hybridisation)   (Gubb, 1998.3.20)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\nec for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) nec-RA FBtr0089052  NM_080112   1621   476
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein nec-PA   FBpp0088123   53.7   476   7.41     NP_524851   AAF59285
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Protease inhibitor I4, serpin (IPR000215) Serpin domain (IPR023796) Protease inhibitor I4, serpin, conserved site (IPR023795)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA697593   AA698400   AA699031   AE013599 AAF59285   AI062016   AI109059   AI113685   AI386991   AI387159   AI387864   AI389919   AI402196   AI403022   AI403391   AI404272   AI513684   AI513761   AJ245444 CAB56697   AX100272   AY102659 AAM27488   AY349666 AAQ64949   AY349667 AAQ64950   AY349668 AAQ64951   AY349669 AAQ64952   AY349670 AAQ64953   AY349671 AAQ64954   AY349672 AAQ64955   AY349673 AAQ64956   AY349674 AAQ64957   AY349675 AAQ64958   BF490505   CA803798   CK659310   CK659443   CO182203   CO282347   CO302539   CO312438   CO316779   CO326979   EC054735   EC062043   EC074383   EC076224   EC238502   EC246222   EC258765   EV578579
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q6V6U1 Q6V6U2 Q6V6U3 Q6V6U4 Q6V6U5 Q6V6U6 Q6V6U7 Q6V6U8 Q6V6U9 Q6V6V0 Q9U5W7 Q7JWX3
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0002930    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0002930 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of extremely low expression. Peak expression observed at stages throughout the pupal period, in stages of adults of both sexes. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0002930 expression level Developmental Stage   Expression Level embryo 00-02hr    7 embryo 02-04hr    11 embryo 04-06hr    31 embryo 06-08hr    95 embryo 08-10hr    145 embryo 10-12hr    558 embryo 12-14hr    716 embryo 14-16hr    912 embryo 16-18hr    1019 embryo 18-20hr    3333 embryo 20-22hr    3750 embryo 22-24hr    2609 larva L1    1286 larva L2    1627 larva L3 12hr old    2876 larva L3 puffstage 1-2    2717 larva L3 puffstage 3-6    2980 larva L3 puffstage 7-9    1513 white prepupae new    1504 white prepupae 12hr    6691 white prepupae 24hr    3629 pupae 2d postWPP    3448 pupae 3d postWPP    1926 pupae 4d postWPP    9100 adult male 01day    6690 adult male 05day    6652 adult male 30day    6586 adult female 01day    4073 adult female 05day    2498 adult female 30day    4346 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr    7 embryo 02-04hr    11 embryo 04-06hr    31 embryo 06-08hr    95 embryo 08-10hr  (145) embryo 10-12hr  (558) embryo 12-14hr  (716) embryo 14-16hr  (912) embryo 16-18hr  (1019) embryo 18-20hr  (3333) embryo 20-22hr  (3750) embryo 22-24hr  (2609) larva L1  (1286) larva L2  (1627) larva L3 12hr old  (2876) larva L3 puffstage 1-2  (2717) larva L3 puffstage 3-6  (2980) larva L3 puffstage 7-9  (1513) white prepupae new  (1504) white prepupae 12hr  (6691) white prepupae 24hr  (3629) pupae 2d postWPP  (3448) pupae 3d postWPP  (1926) pupae 4d postWPP  (9100) adult male 01day  (6690) adult male 05day  (6652) adult male 30day  (6586) adult female 01day  (4073) adult female 05day  (2498) adult female 30day  (4346) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    7 embryo 02-04hr    11 embryo 04-06hr    31 embryo 06-08hr    95 embryo 08-10hr    145 embryo 10-12hr    558 embryo 12-14hr    716 embryo 14-16hr    912 embryo 16-18hr    1019 embryo 18-20hr  (3333) embryo 20-22hr  (3750) embryo 22-24hr  (2609) larva L1    1286 larva L2  (1627) larva L3 12hr old  (2876) larva L3 puffstage 1-2  (2717) larva L3 puffstage 3-6  (2980) larva L3 puffstage 7-9  (1513) white prepupae new  1504 white prepupae 12hr  (6691) white prepupae 24hr  (3629) pupae 2d postWPP  (3448) pupae 3d postWPP  (1926) pupae 4d postWPP  (9100) adult male 01day  (6690) adult male 05day  (6652) adult male 30day  (6586) adult female 01day  (4073) adult female 05day  (2498) adult female 30day  (4346) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    7 embryo 02-04hr    11 embryo 04-06hr    31 embryo 06-08hr    95 embryo 08-10hr    145 embryo 10-12hr    558 embryo 12-14hr    716 embryo 14-16hr    912 embryo 16-18hr    1019 embryo 18-20hr    3333 embryo 20-22hr    3750 embryo 22-24hr    2609 larva L1    1286 larva L2    1627 larva L3 12hr old    2876 larva L3 puffstage 1-2    2717 larva L3 puffstage 3-6    2980 larva L3 puffstage 7-9    1513 white prepupae new    1504 white prepupae 12hr    6691 white prepupae 24hr    3629 pupae 2d postWPP    3448 pupae 3d postWPP    1926 pupae 4d postWPP    9100 adult male 01day    6690 adult male 05day    6652 adult male 30day    6586 adult female 01day    4073 adult female 05day    2498 adult female 30day    4346 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    7 embryo 02-04hr    11 embryo 04-06hr    31 embryo 06-08hr    95 embryo 08-10hr    145 embryo 10-12hr    558 embryo 12-14hr    716 embryo 14-16hr    912 embryo 16-18hr    1019 embryo 18-20hr    3333 embryo 20-22hr    3750 embryo 22-24hr    2609 larva L1    1286 larva L2    1627 larva L3 12hr old    2876 larva L3 puffstage 1-2    2717 larva L3 puffstage 3-6    2980 larva L3 puffstage 7-9    1513 white prepupae new    1504 white prepupae 12hr    6691 white prepupae 24hr    3629 pupae 2d postWPP    3448 pupae 3d postWPP    1926 pupae 4d postWPP    9100 adult male 01day    6690 adult male 05day    6652 adult male 30day    6586 adult female 01day    4073 adult female 05day    2498 adult female 30day    4346 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0002930 expression level Developmental Stage   Expression Level embryo 00-02hr    7 embryo 02-04hr    11 embryo 04-06hr    31 embryo 06-08hr    95 embryo 08-10hr    145 embryo 10-12hr    558 embryo 12-14hr    716 embryo 14-16hr    912 embryo 16-18hr    1019 embryo 18-20hr    3333 embryo 20-22hr    3750 embryo 22-24hr    2609 larva L1    1286 larva L2    1627 larva L3 12hr old    2876 larva L3 puffstage 1-2    2717 larva L3 puffstage 3-6    2980 larva L3 puffstage 7-9    1513 white prepupae new    1504 white prepupae 12hr    6691 white prepupae 24hr    3629 pupae 2d postWPP    3448 pupae 3d postWPP    1926 pupae 4d postWPP    9100 adult male 01day    6690 adult male 05day    6652 adult male 30day    6586 adult female 01day    4073 adult female 05day    2498 adult female 30day    4346 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr    7 embryo 02-04hr    11 embryo 04-06hr    31 embryo 06-08hr    95 embryo 08-10hr  (145) embryo 10-12hr  (558) embryo 12-14hr  (716) embryo 14-16hr  (912) embryo 16-18hr  (1019) embryo 18-20hr  (3333) embryo 20-22hr  (3750) embryo 22-24hr  (2609) larva L1  (1286) larva L2  (1627) larva L3 12hr old  (2876) larva L3 puffstage 1-2  (2717) larva L3 puffstage 3-6  (2980) larva L3 puffstage 7-9  (1513) white prepupae new  (1504) white prepupae 12hr  (6691) white prepupae 24hr  (3629) pupae 2d postWPP  (3448) pupae 3d postWPP  (1926) pupae 4d postWPP  (9100) adult male 01day  (6690) adult male 05day  (6652) adult male 30day  (6586) adult female 01day  (4073) adult female 05day  (2498) adult female 30day  (4346) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    7 embryo 02-04hr    11 embryo 04-06hr    31 embryo 06-08hr    95 embryo 08-10hr    145 embryo 10-12hr    558 embryo 12-14hr    716 embryo 14-16hr    912 embryo 16-18hr    1019 embryo 18-20hr  (3333) embryo 20-22hr  (3750) embryo 22-24hr  (2609) larva L1    1286 larva L2  1627 larva L3 12hr old  (2876) larva L3 puffstage 1-2  (2717) larva L3 puffstage 3-6  (2980) larva L3 puffstage 7-9  1513 white prepupae new  1504 white prepupae 12hr  (6691) white prepupae 24hr  (3629) pupae 2d postWPP  (3448) pupae 3d postWPP  1926 pupae 4d postWPP  (9100) adult male 01day  (6690) adult male 05day  (6652) adult male 30day  (6586) adult female 01day  (4073) adult female 05day  (2498) adult female 30day  (4346) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    7 embryo 02-04hr    11 embryo 04-06hr    31 embryo 06-08hr    95 embryo 08-10hr    145 embryo 10-12hr    558 embryo 12-14hr    716 embryo 14-16hr    912 embryo 16-18hr    1019 embryo 18-20hr    3333 embryo 20-22hr    3750 embryo 22-24hr    2609 larva L1    1286 larva L2    1627 larva L3 12hr old    2876 larva L3 puffstage 1-2    2717 larva L3 puffstage 3-6    2980 larva L3 puffstage 7-9    1513 white prepupae new    1504 white prepupae 12hr    6691 white prepupae 24hr    3629 pupae 2d postWPP    3448 pupae 3d postWPP    1926 pupae 4d postWPP    9100 adult male 01day    6690 adult male 05day    6652 adult male 30day    6586 adult female 01day    4073 adult female 05day    2498 adult female 30day    4346 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    7 embryo 02-04hr    11 embryo 04-06hr    31 embryo 06-08hr    95 embryo 08-10hr    145 embryo 10-12hr    558 embryo 12-14hr    716 embryo 14-16hr    912 embryo 16-18hr    1019 embryo 18-20hr    3333 embryo 20-22hr    3750 embryo 22-24hr    2609 larva L1    1286 larva L2    1627 larva L3 12hr old    2876 larva L3 puffstage 1-2    2717 larva L3 puffstage 3-6    2980 larva L3 puffstage 7-9    1513 white prepupae new    1504 white prepupae 12hr    6691 white prepupae 24hr    3629 pupae 2d postWPP    3448 pupae 3d postWPP    1926 pupae 4d postWPP    9100 adult male 01day    6690 adult male 05day    6652 adult male 30day    6586 adult female 01day    4073 adult female 05day    2498 adult female 30day    4346 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0002930    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0002930 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, adult crop, adult heart, larval/adult fat body, adult spermathecae, adult carcass. Expression at moderate levels in the following post-embryonic organs or tissues: adult central nervous system, larval/adult hindgut. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0002930 expression level Tissue   Expression Level Larval Central Nervous System no informative data Larval Midgut    2.4 Larval Hindgut    106.7 Larval Malpighian Tubules    5.9 Larval Fat Body    1114.9 Larval Salivary Gland no informative data Larval Trachea    42.425 Larval Carcass    47.8 Adult Head    1031.2 Adult Eye    619.1 Adult Brain    273 Adult Thoracic-Abdominal Ganglion    335.8 Adult Crop    587.7 Adult Midgut no informative data Adult Hindgut    419.1 Adult Malpighian Tubules no informative data Adult Fat Body    1884.1 Adult Salivary Gland no informative data Adult Heart    1113.9 Adult VirginFemale Spermatheca    1157.4 Adult InseminatedFemale Spermatheca    883.6 Adult Ovary    4 Adult Testis    63.9 Adult Male Accessory Gland    90.5 Adult Carcass    1204.5 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System no informative data Larval Midgut    2.4 Larval Hindgut    106.7 Larval Malpighian Tubules    5.9 Larval Fat Body  (1114.9) Larval Salivary Gland no informative data Larval Trachea    42.425 Larval Carcass    47.8 Adult Head  (1031.2) Adult Eye  (619.1) Adult Brain    273 Adult Thoracic-Abdominal Ganglion    335.8 Adult Crop  (587.7) Adult Midgut no informative data Adult Hindgut    419.1 Adult Malpighian Tubules no informative data Adult Fat Body  (1884.1) Adult Salivary Gland no informative data Adult Heart  (1113.9) Adult VirginFemale Spermatheca  (1157.4) Adult InseminatedFemale Spermatheca  (883.6) Adult Ovary    4 Adult Testis    63.9 Adult Male Accessory Gland    90.5 Adult Carcass  (1204.5) Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System no informative data Larval Midgut    2.4 Larval Hindgut    106.7 Larval Malpighian Tubules    5.9 Larval Fat Body  (1114.9) Larval Salivary Gland no informative data Larval Trachea    42.425 Larval Carcass    47.8 Adult Head  1031.2 Adult Eye    619.1 Adult Brain    273 Adult Thoracic-Abdominal Ganglion    335.8 Adult Crop    587.7 Adult Midgut no informative data Adult Hindgut    419.1 Adult Malpighian Tubules no informative data Adult Fat Body  (1884.1) Adult Salivary Gland no informative data Adult Heart  (1113.9) Adult VirginFemale Spermatheca  (1157.4) Adult InseminatedFemale Spermatheca    883.6 Adult Ovary    4 Adult Testis    63.9 Adult Male Accessory Gland    90.5 Adult Carcass  (1204.5) Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System no informative data Larval Midgut    2.4 Larval Hindgut    106.7 Larval Malpighian Tubules    5.9 Larval Fat Body    1114.9 Larval Salivary Gland no informative data Larval Trachea    42.425 Larval Carcass    47.8 Adult Head    1031.2 Adult Eye    619.1 Adult Brain    273 Adult Thoracic-Abdominal Ganglion    335.8 Adult Crop    587.7 Adult Midgut no informative data Adult Hindgut    419.1 Adult Malpighian Tubules no informative data Adult Fat Body    1884.1 Adult Salivary Gland no informative data Adult Heart    1113.9 Adult VirginFemale Spermatheca    1157.4 Adult InseminatedFemale Spermatheca    883.6 Adult Ovary    4 Adult Testis    63.9 Adult Male Accessory Gland    90.5 Adult Carcass    1204.5 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0002930 expression level Tissue   Expression Level Larval Central Nervous System no informative data Larval Midgut    2.4 Larval Hindgut    106.7 Larval Malpighian Tubules    5.9 Larval Fat Body    1114.9 Larval Salivary Gland no informative data Larval Trachea    42.425 Larval Carcass    47.8 Adult Head    1031.2 Adult Eye    619.1 Adult Brain    273 Adult Thoracic-Abdominal Ganglion    335.8 Adult Crop    587.7 Adult Midgut no informative data Adult Hindgut    419.1 Adult Malpighian Tubules no informative data Adult Fat Body    1884.1 Adult Salivary Gland no informative data Adult Heart    1113.9 Adult VirginFemale Spermatheca    1157.4 Adult InseminatedFemale Spermatheca    883.6 Adult Ovary    4 Adult Testis    63.9 Adult Male Accessory Gland    90.5 Adult Carcass    1204.5 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System no informative data Larval Midgut    2.4 Larval Hindgut    106.7 Larval Malpighian Tubules    5.9 Larval Fat Body  (1114.9) Larval Salivary Gland no informative data Larval Trachea    42.425 Larval Carcass    47.8 Adult Head  (1031.2) Adult Eye  619.1 Adult Brain    273 Adult Thoracic-Abdominal Ganglion    335.8 Adult Crop  587.7 Adult Midgut no informative data Adult Hindgut    419.1 Adult Malpighian Tubules no informative data Adult Fat Body  (1884.1) Adult Salivary Gland no informative data Adult Heart  (1113.9) Adult VirginFemale Spermatheca  (1157.4) Adult InseminatedFemale Spermatheca  (883.6) Adult Ovary    4 Adult Testis    63.9 Adult Male Accessory Gland    90.5 Adult Carcass  (1204.5) Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System no informative data Larval Midgut    2.4 Larval Hindgut    106.7 Larval Malpighian Tubules    5.9 Larval Fat Body  1114.9 Larval Salivary Gland no informative data Larval Trachea    42.425 Larval Carcass    47.8 Adult Head  1031.2 Adult Eye    619.1 Adult Brain    273 Adult Thoracic-Abdominal Ganglion    335.8 Adult Crop    587.7 Adult Midgut no informative data Adult Hindgut    419.1 Adult Malpighian Tubules no informative data Adult Fat Body  (1884.1) Adult Salivary Gland no informative data Adult Heart  1113.9 Adult VirginFemale Spermatheca  1157.4 Adult InseminatedFemale Spermatheca    883.6 Adult Ovary    4 Adult Testis    63.9 Adult Male Accessory Gland    90.5 Adult Carcass  1204.5 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System no informative data Larval Midgut    2.4 Larval Hindgut    106.7 Larval Malpighian Tubules    5.9 Larval Fat Body    1114.9 Larval Salivary Gland no informative data Larval Trachea    42.425 Larval Carcass    47.8 Adult Head    1031.2 Adult Eye    619.1 Adult Brain    273 Adult Thoracic-Abdominal Ganglion    335.8 Adult Crop    587.7 Adult Midgut no informative data Adult Hindgut    419.1 Adult Malpighian Tubules no informative data Adult Fat Body    1884.1 Adult Salivary Gland no informative data Adult Heart    1113.9 Adult VirginFemale Spermatheca    1157.4 Adult InseminatedFemale Spermatheca    883.6 Adult Ovary    4 Adult Testis    63.9 Adult Male Accessory Gland    90.5 Adult Carcass    1204.5 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System no informative data Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland no informative data Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut no informative data Adult Hindgut     Adult Malpighian Tubules no informative data Adult Fat Body     Adult Salivary Gland no informative data Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    1031.2   NA  Eye    619.1   NA  Brain    273   no informative data  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    335.8   NA  Crop    587.7   2.4  Midgut    no informative data   106.7  Hindgut    419.1   5.9  Malpighian Tubules    no informative data   1114.9  Fat Body    1884.1   no informative data  Salivary Gland    no informative data   NA  Heart    1113.9   42.425  Trachea    NA   NA  VirginFemale Spermatheca    1157.4   NA  InseminatedFemale Spermatheca    883.6   NA  Ovary    4   NA  Testis    63.9   NA  Male Accessory Gland    90.5   47.8  Carcass    1204.5 modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_10
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0002930 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG1857-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal necunspecified lethal, with Scer\GAL4pnr-MD237 necGD5273 lethal | adult stage (with nec1) nec2 lethal | adult stage (with nec2) nec1 lethal | recessive nec1 nec2 nec4 nec6 nec7 nec8 nec9 nec10 nec11 nec12 nec13 semi-viable, with Scer\GAL4Act5C.PI nec1.Scer\UAS nec9.Scer\UAS necScer\UAS.cLa semi-viable, with Scer\GAL4yolk nec1.Scer\UAS nec9.Scer\UAS necScer\UAS.cLa necSF.Scer\UAS semi-viable | adult stage | heat sensitive (with nec1) nec2 semi-viable | adult stage | heat sensitive (with nec2) nec1 nec9 semi-viable | adult stage | heat sensitive (with nec9) nec2 semi-viable | heat sensitive, with Scer\GAL4Act5C.PI necSF.Scer\UAS Sterility Allele female sterile (with nec1) nec2 female sterile (with nec2) nec1 Other Phenotypes Allele melanotic necrosis, with Scer\GAL4Act5C.PI necdsRNA.L.Scer\UAS necdsRNA.S.Scer\UAS melanotic necrosis (with nec1) nec2 melanotic necrosis (with nec2) nec1 non-enhancer of variegation, with In(1)wm4 nec1 non-suppressor of variegation, with In(1)wm4 nec1 short lived, with Scer\GAL4Act5C.PI nec1.Scer\UAS nec9.Scer\UAS necScer\UAS.cLa short lived, with Scer\GAL4yolk nec1.Scer\UAS nec9.Scer\UAS necScer\UAS.cLa necSF.Scer\UAS short lived (with nec1) nec2 short lived (with nec2) nec1 short lived | heat sensitive, with Scer\GAL4Act5C.PI necSF.Scer\UAS short lived | heat sensitive (with nec1) nec2 short lived | heat sensitive (with nec2) nec1 nec9 short lived | heat sensitive (with nec9) nec2 visible necunspecified wild-type nec+t7.0 Phenotype manifest in Allele adult abdomen (with nec1) nec2 adult abdomen (with nec2) nec1 adult cuticle (with nec2) nec19 adult cuticle (with nec19) nec2 adult epidermis necunspecified adult epidermis (with nec1) nec2 adult epidermis (with nec2) nec1 adult integumentary system necunspecified joint necunspecified
Classical Alleles ( 22 )
For All Classical Alleles Show
Allele of nec Class Mutagen Stocks Known lesion nec10 amorphic allele - genetic evidence ethyl methanesulfonate 2 Yes nec1 amorphic allele - genetic evidence ethyl methanesulfonate 1 Yes nec2 amorphic allele - genetic evidence ethyl methanesulfonate 1 Yes necJR 1 -- nec11 amorphic allele - genetic evidence ethyl methanesulfonate 0 -- nec12 amorphic allele - genetic evidence ethyl methanesulfonate 0 -- nec13 amorphic allele - genetic evidence ethyl methanesulfonate 0 -- nec4 amorphic allele - genetic evidence ethyl methanesulfonate 0 -- nec6 amorphic allele - genetic evidence ethyl methanesulfonate 0 -- nec7 amorphic allele - genetic evidence ethyl methanesulfonate 0 Yes nec8 amorphic allele - genetic evidence ethyl methanesulfonate 0 -- nec9 amorphic allele - genetic evidence ethyl methanesulfonate 0 Yes nec15 X ray 0 -- nec16 X ray ethyl methanesulfonate 0 Yes nec17 X ray 0 -- nec18 X ray 0 -- nec19 X ray ethyl methanesulfonate 0 Yes nec20 X ray ethyl methanesulfonate 0 Yes nec22 X ray ethyl methanesulfonate 0 Yes necMtnA.T:SV5\V5,T:Zzzz\His6 0 Yes necNpk ethyl methanesulfonate 0 -- necunspecified 0 --
Alleles Carried on Transgenic Constructs ( 13 )
For All Alleles Carried on Transgenic Constructs Show
Allele of nec Class Mutagen Stocks Known lesion necGD5273 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes necKK105206 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes nec+t7.0 in vitro construct - genomic fragment 0 Yes nec1.Scer\UAS in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes nec9.Scer\UAS in vitro construct - regulatory fusion in vitro construct - site directed mutagenesis 0 Yes necdsRNA.57-1473 in vitro construct - RNAi 0 Yes necdsRNA.CopT.Scer\UAS in vitro construct - regulatory fusion in vitro construct - RNAi 0 Yes necdsRNA.L.Scer\UAS in vitro construct - regulatory fusion in vitro construct - RNAi 0 Yes necdsRNA.S.Scer\UAS in vitro construct - regulatory fusion in vitro construct - RNAi 0 Yes necK68Q.K70Q.K71Q.Scer\UAS in vitro construct - regulatory fusion 0 Yes necPP.Scer\UAS in vitro construct - regulatory fusion in vitro construct - amino acid replacement 0 Yes necScer\UAS.cLa in vitro construct - regulatory fusion 0 Yes necSF.Scer\UAS in vitro construct - regulatory fusion in vitro construct - site directed mutagenesis 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2R)cn-S5 (Heitzler et al., 1993) Df(2R)Drlrv3 (Heitzler et al., 1993) Df(2R)Drlrv5 (Heitzler et al., 1993) Df(2R)Drlrv7 (Heitzler et al., 1993) Df(2R)Drlrv15 (Heitzler et al., 1993) Df(2R)Drlrv17 (Heitzler et al., 1993) Df(2R)Drlrv18 (Heitzler et al., 1993) Df(2R)Drlrv20 (Heitzler et al., 1993) Df(2R)Drlrv24 (Heitzler et al., 1993) Df(2R)Drlrv25 (Heitzler et al., 1993) Df(2R)Drlrv26 (Heitzler et al., 1993) Df(2R)Drlrv28 (Heitzler et al., 1993) Df(2R)nap2 (Levashina et al., 1999, Green et al., 2000, Heitzler et al., 1993) Df(2R)Npk-rv1 (Roote and Ashburner, 2005.6) Df(2R)Npk-rv2 (Roote and Ashburner, 2005.6) Df(2R)Npk-rv4 (Roote and Ashburner, 2005.6) Df(2R)Npk-rv5 (Roote and Ashburner, 2005.6) Df(2R)Npk-rv6 (Roote and Ashburner, 2005.6) Df(2R)Npk-rv7 (Roote and Ashburner, 2005.6) Df(2R)pk78k (Green et al., 2000) Df(2R)pk-N5 (Heitzler et al., 1993) Df(2R)pk-sple-50 (Gubb et al., 1999) Df(2R)pk-sple-51 (Gubb et al., 1999) Df(2R)sple-D1 (Green et al., 2000, Heitzler et al., 1993) Df(2R)sple-D2 (Green et al., 2000, Heitzler et al., 1993) Df(2R)sple-D3 (Heitzler et al., 1993, Roote, 1996.8) Df(2R)sple-J1 (Levashina et al., 1999, Heitzler et al., 1993) Df(2R)sple-J2 (Heitzler et al., 1993) Df(2R)sple-N3 (Heitzler et al., 1993) In(2LR)pk44 (Gubb et al., 1999) In(2LR)TE35B-14LScorv1R (Roote et al., 1996, Roote, 1996.8) In(2LR)TE35B-14LTE35B-4R (Roote, 1996.8, Roote, 1997.6.6) In(2R)nec-5 (Gubb et al., 1999) In(2R)nec-e3 (Gubb et al., 1999) T(2;3)pk-sple31 (Gubb et al., 1999) T(Y;2)pk-sple45 (Gubb et al., 1999) Tp(2;2)pk-sple22 (Gubb et al., 1999) Tp(2;2)pk-sple24 (Gubb et al., 1999)
Not disrupted in	Df(2R)cn-S3 (Heitzler et al., 1993) Df(2R)cn-S6 (Heitzler et al., 1993) Df(2R)cn-S8 (Heitzler et al., 1993) Df(2R)cos-2 (Heitzler et al., 1993) Df(2R)cos-3 (Heitzler et al., 1993) Df(2R)Drlrv21 (Heitzler et al., 1993) Df(2R)Drlrv22 (Heitzler et al., 1993) Df(2R)Drlrv30 (Heitzler et al., 1993) Df(2R)DX1 (Heitzler et al., 1993) Df(2R)DX14 (Heitzler et al., 1993) Df(2R)EW60 (Heitzler et al., 1993) Df(2R)H23 (Heitzler et al., 1993) Df(2R)NCX5 (Heitzler et al., 1993) Df(2R)NCX8 (Heitzler et al., 1993) Df(2R)NCX9 (Heitzler et al., 1993) Df(2R)NCX10 (Heitzler et al., 1993) Df(2R)NCX11 (Heitzler et al., 1993) Df(2R)NCX13 (Heitzler et al., 1993) Df(2R)Npk-rv3 (Roote and Ashburner, 2005.6) Df(2R)pwn5 (Heitzler et al., 1993) Df(2R)tor-rx6 (Heitzler et al., 1993)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD5273} NA P{KK105206} NA P{UAS-nec.1} NA P{UAS-nec.9} NA P{UAS-nec.K68Q.K70Q.K71Q} NA P{UAS-nec.L} NA P{UAS-nec.L-dsRNA} NA P{UAS-nec.S-dsRNA} NA P{UAS-nec.SF} NA P{UAS-necPP.G} NA P{UAS-nec-CopT-dsRNA} NA characterization construct P{nec.7.0.G} NA P{UAS-f::nec-dsRNA} NA
Insertions
	Type of insertions Name Expression data
Gene Ontology: Function, Process & Cellular Component ( 7 unique terms )
Terms Based on Experimental Evidence ( 5 terms )
Molecular Function
CV term Evidence References
serine-type endopeptidase inhibitor activity inferred from direct assay (Robertson et al., 2003)
Biological Process
CV term Evidence References
antifungal humoral response inferred from genetic interaction with spz AND traceable author statement (Green et al., 2000) immune response inferred from expression pattern (Levashina et al., 1999) negative regulation of Toll signaling pathway inferred from mutant phenotype (Levashina et al., 1999) regulation of proteolysis inferred from direct assay (Robertson et al., 2003) inferred from mutant phenotype (Levashina et al., 1999)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 3 terms )
Molecular Function
CV term Evidence References
serine-type endopeptidase inhibitor activity inferred from sequence or structural similarity with EMBL:U58361 (FlyBase, 1992-) non-traceable author statement (Levashina et al., 1998, Leclerc and Reichhart, 2004)
Biological Process
CV term Evidence References
defense response non-traceable author statement (Lemaitre, 2000.12.20) Toll signaling pathway non-traceable author statement (Lemaitre, 2000.12.20) traceable author statement (Kimbrell and Beutler, 2001, Carrell and Corral, 2004)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions nec allele Gene References nec1 psh (Ligoxygakis et al., 2002) spz (Green et al., 2000) nec2 psh (Ligoxygakis et al., 2002) spz (Green et al., 2000)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 69974 DroID - A comprehensive database of gene and protein interactions. • FBgn0002930 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 45908
Orthologs
Genome-wide drosophilid orthologs	Dana\GF12243 Dere\GG10764 Dgri\GH20376 Dmoj\GI20049 Dper\GL10947 Dpse\GA14995 Dsec\GM20812 Dsim\nec Dvir\GJ14938 Dwil\GK21526 Dyak\nec
Curated drosophilid orthologs	Dmoj\nec (Lee et al., 2007) Dpse\GA14995   Dsim\nec   Dyak\nec
Linkouts	OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • FBgn0067427 FBgn0223518 FBgn0202141 FBgn0067495 FBgn0175693 FBgn0075020 FBgn0148557 FBgn0142785 FBgn0127839 FBgn0103069 FBgn0089281
Stocks & Reagents
Stocks Listed in FlyBase ( 8 )
Bloomington	9151 Dp(?;2)bwD, nec2 bwD/CyO 4288 nec10 cn1 bw1 sp1/CyO 11382 P{PZ}l(2)43Bb04614a cn1/CyO, necJR; ry506 P{PZ}stg04614b
Kyoto	107672 nec10 cn1 bw1 sp1/CyO 101403 nec1 bwD / CyO, Tp(2;2)pk-sple26
VDRC	v108366 P{KK105206}VIE-260B v37958 w1118; P{GD5273}v37958 v44837 w1118; P{GD5273}v44837
Genomic Clones ( 1 )
	BACR12F04 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 33 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GH10112
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT26992 GH01054 GH07566 GH17392 GH17608 GH18542 GH21227 GH22377 GH22817 GH23919 GH26737 GH26837 HL02838 HL04407 HL07334 LP16986 LP17213
Other clones	44021 59496 60457 101917 161390 214435.40 Dmel_dig_000401_0627_0323 EC22258 EC054735 EK160259 EK184358 EK228811 EK265838 EP05241 ESG0110c.E21_E09
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0002930 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 45908
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: nec anon-WO0118057.3 (Gubb, 1998.3.20, FlyBase, 1996-) Source for merge of: nec CG1857 (Lemaitre, 2000.9.1)
Additional comments	Source for merge of nec anon-WO0118057.3 was sequence comparison (date:030912). (Gubb, 1998.3.20, FlyBase, 1996-)
Other Comments
	nec requires spe, sphinx2, sphinx1, spheroide, grass, and spirit to activate Tl. (Kambris et al., 2006) The Tl-mediated immune response to fungal infections is constitutively activated in nec mutants and pleiotropic phenotypes include melanization and cellular necrosis. (Green et al., 2000) nec negatively regulates the Tl signalling pathway. (Levashina et al., 1999) nec has a role in Tl mediated antifungal defence. (Levashina et al., 1999) Deletion mutants for all three serpins (Spn43Aa, Spn43Ab and nec) survive as adults for a short while before dying with an extreme nec phenotype. (Gubb, 1998.3.20) The cellular polarity gene pk and the adult lethal nec gene map within the proximal region of pk-sple complex among a set of 3 Serpin transcripts. (Gubb and Green, 1995)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_080112 RefSeq (Proteins) • NP_524851 DDBJ / EMBL / GenBank • AAF59285 AAM27488 AAQ64949 AAQ64950 AAQ64951 AAQ64952 AAQ64953 AAQ64954 AAQ64955 AAQ64956 AAQ64957 AAQ64958 AI113685 AI402196 AJ245444 AX100272 AY102659 AY349666 AY349667 AY349668 AY349669 AY349670 AY349671 AY349672 AY349673 AY349674 AY349675 CAB56697 Entrez Gene - A searchable database of RefSeq genes. • 45908 UniProtKB/TrEMBL • Q6V6U1 Q6V6U2 Q6V6U3 Q6V6U4 Q6V6U5 Q6V6U6 Q6V6U7 Q6V6U8 Q6V6U9 Q6V6V0 Q7JWX3 Q9U5W7
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Protease inhibitor I4, serpin (IPR000215) Serpin domain (IPR023796) Protease inhibitor I4, serpin, conserved site (IPR023795)
Linkouts
	BioGRID - A database of protein and genetic interactions • 69974 DroID - A comprehensive database of gene and protein interactions. • FBgn0002930 DRSC - Results from RNAi screens. • FBgn0002930 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0002930 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG1857-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0002930 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 45908 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 45908 modMine - Data generated by the modENCODE project. • FBgn0002930 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • FBgn0067427 FBgn0223518 FBgn0202141 FBgn0067495 FBgn0175693 FBgn0075020 FBgn0148557 FBgn0142785 FBgn0127839 FBgn0103069 FBgn0089281
Synonyms & Secondary IDs ( 12 )
Reported As
Symbol Synonym	anon-WO0118057.3   CG1857 (Irving et al., 2001, De Gregorio et al., 2001, Lemaitre, 2000.9.1, Pal et al., 2007, Carrera et al., 2008, Keleman et al., 2009.8.5, Easow et al., 2007, Hernandez-Romano et al., 2008, Garrett et al., 2009, Wertheim et al., 2005) nec (Kambris et al., 2006, Lee et al., 2007, Kambris et al., 2006, Blanco et al., 2010, Lee et al., 2007, Robertson et al., 2006, Christophides et al., 2002, Carrera et al., 2008, Soukup et al., 2009, Scherfer et al., 2008, Wertheim et al., 2005, Yan et al., 2010) Nec (Pal et al., 2007, Shin, 2005, Soukup and Gubb, 2007, Waterhouse et al., 2007, Pelte et al., 2006, Soukup and Gubb, 2008, Busse et al., 2007, Ahmad et al., 2009, Obbard et al., 2009, Avet-Rochex et al., 2010) SER3 (Reichhart et al., 1998) Ser3 (Levashina et al., 1998) Spn43Ac (Goto et al., 2003, De Gregorio et al., 2002, Kimbrell and Beutler, 2001, Imler and Hoffmann, 2001, Silverman et al., 2001, Gubb et al., 2001, Gubb, 1999.7.27, Green et al., 2000, Barillas-Mury et al., 2000, Jiang and Kanost, 2000, Levashina et al., 1999, Gubb et al., 1999, Tang et al., 2007, Ahmad et al., 2009, Garrett et al., 2009)
Name Synonym	necrotic (Pal et al., 2007, Soukup and Gubb, 2008, Zhou et al., 2008, Buchon et al., 2009, Crowther et al., 2005) Necrotic (Lee et al., 2007, Robertson et al., 2006, Roxstrom-Lindquist et al., 2004, Ahmad et al., 2009, Hernandez-Romano et al., 2008, Zsindely et al., 2009) necrotic serpin (Tang et al., 2007) Serine protease inhibitor 3 (Reichhart et al., 1998) serpin43Ac (Lemaitre, 2000.12.20)
Secondary FlyBase IDs
	FBgn0024292 FBgn0028676 FBgn0033148 FBgn0063807
References ( 96 )
Generate a list of	All references relating to this gene ( 96 )
List References by type	Research paper  ( 42 ) Supplementary material  ( 3 ) Review  ( 18 ) Personal communication to FlyBase  ( 11 ) Abstract  ( 14 ) FlyBase analysis  ( 2 ) DNA/RNA sequence record  ( 1 ) Letter  ( 1 ) Computer file  ( 1 ) Curated genome annotation  ( 1 ) Automatic genome annotation  ( 1 ) Patent  ( 1 )
Recent research papers ( 4 )
	Baycin-Hizal et al., 2011, J. Proteome Res. 10(6): 2777--2784 GlycoFly: A Database of Drosophila N-linked Glycoproteins Identified Using SPEG-MS Techniques. [FBrf0213834] Avet-Rochex et al., 2010, BMC Dev. Biol. 10: 65 An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis. [FBrf0211137] Blanco et al., 2010, BMC Dev. Biol. 10: 94 Gene expression following induction of regeneration in Drosophila wing imaginal discs. Expression profile of regenerating wing discs. [FBrf0211812] Yan et al., 2010, PLoS ONE 5(8): e12139 A Genome-Wide Gene Function Prediction Resource for Drosophila melanogaster. [FBrf0211503] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...