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Gene Dmel\net

General Information
Symbol	Dmel\net	Species	D. melanogaster
Name	net	Annotation symbol	CG11450
Feature type	protein_coding_gene	FlyBase ID	FBgn0002931
Gene Model Status	Current	Stock availability	29 publicly available
Genomic Location
Chromosome (arm)	2L	Recombination map	2-0.0
Cytogenetic map	21B1-21B1	Sequence location	2L:82,421..87,387 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene net is referred to in FlyBase by the symbol Dmel\net (CG11450, FBgn0002931). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: sequence-specific DNA binding transcription factor activity. There is experimental evidence that it is involved in the biological process: imaginal disc-derived wing vein specification; negative regulation of transcription from RNA polymerase II promoter. 37 alleles are reported. The phenotypes of these alleles are annotated with: adult segment; organ system subdivision; thoracic segment; organ system; adult; region of integument; portion of tissue; acellular anatomical structure; wing vein L3; wing vein L5; tagmatic subdivision of integument; marginal cell. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: Myc-type, basic helix-loop-helix (bHLH) domain. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderate expression to a trough of very low expression. Peak expression observed within 00-06 hour embryonic stages, during late pupal stages. Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: larval salivary gland. Expression at moderate levels in the following post-embryonic organs or tissues: adult salivary gland. Comments on Affy2 ProbeSet: ProbeSet 1627446_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of net. Gene sequence location is 2L:82421..87387.
User Contributed Data
	Beta version, feedback welcome
External Summaries
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	net: net net: net Edith M. Wallace, unpublished. Wing veins form plexus-like net; first posterior cell between L3 and L4 widens toward tip; branch missing from posterior crossvein; all veins fused at base of wing, like bi. According to Waddington [1940, J. Genet. 41: 75-139 (fig.)], spaces form between epithelial layers owing to inadequate contraction during pupal period; spaces later fuse and form extra veins (Diaz-Benjumea, Gonzalez-Gaitan, and Garcia-Bellido, 1989, Genome 31: 612-19). RK1.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 21B1-21B1   Limits computationally determined from genome sequence between P{lacW}spenk06805&P{lacW}l(2)k13604k13604 and P{lacW}U2af38k14504
Experimentally Determined Cytological Location
Cytogenetic map Notes References 21A1-21B3     (Golubovsky et al., 1978)
Experimentally Determined Recombination Data
Location	2-0 (Golubovsky et al., 1978, Williams, 1956) 2-0.0 (Meyer, 1956) 2- (Kiil, 1946) 2-0.3 (Neel, 1942)
Left of (cM)	l(2)gl (Golubovsky et al., 1978)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\net for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.44 Gene model reviewed during 5.45
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) net-RB FBtr0290323  NM_080081   2225   360 net-RC FBtr0330637  NM_001272860   1662   360
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein net-PB   FBpp0288762   39.1   360   9.82     NP_524820   AAF51562 net-PC   FBpp0303487   39.1   360   9.82     NP_001259789   AGB92326
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Myc-type, basic helix-loop-helix (bHLH) domain (IPR011598)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE014134 AAF51562   AF303350 AAK14073   AE014134 AGB92326   AI386852   BT022740   BT022752   BT050459   BT058006   CO187659   CO265482   CO281279   CO293443   CO303255   CO310321   CO319443   CO321101   CO321951   CO333191   CO342410   CZ478919   CZ478940   EC084721   EC085863   EC198559   EC205359   EC210231   EC224229   EC227285   EC229561   EC247965   EC250225   EC254844   EL874393   EL874546   GH943609   GH945736   GH947206   GH947497   GH948743   GH949148   GH949227   GH949389   GH952502   GH954656   GH959796   GH960341   GH960850   GH962501   GH962709   GH963137   GH963817   GH964256   GH967865   GH968485   GH971733   GH971825   GH973949   GH974712   GH978647   GH979615   GH980583   GH980707   GH981451   GH983306
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q9VPI8 Q7KH24
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 4 -- 6   dorsal ectoderm anlage   (Fisher et al., 2012) Comment:anlage in statu nascendi dorsal head epidermis anlage in statu nascendi   (Fisher et al., 2012) head mesoderm anlage   (Fisher et al., 2012) trunk mesoderm anlage   (Fisher et al., 2012) Comment:anlage in statu nascendi embryonic stage 7 -- 8   amnioserosa anlage   (Fisher et al., 2012) dorsal ectoderm   (Fisher et al., 2012) head mesoderm   (Fisher et al., 2012) trunk mesoderm   (Fisher et al., 2012) embryonic stage 9 -- 10   amnioserosa primordium   (Fisher et al., 2012) head mesoderm   (Fisher et al., 2012) trunk mesoderm   (Fisher et al., 2012) embryonic stage 11 -- 12   fat body/gonad primordium   (Fisher et al., 2012) head mesoderm   (Fisher et al., 2012) salivary gland body primordium   (Fisher et al., 2012) somatic muscle primordium   (Fisher et al., 2012) Comment:reported as muscle system primordium visceral muscle primordium   (Fisher et al., 2012) Comment:reported as muscle system primordium trunk mesoderm   (Fisher et al., 2012) embryonic stage 13 -- 16   embryonic/larval salivary gland body   (Fisher et al., 2012)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: larval salivary gland. Expression at moderate levels in the following post-embryonic organs or tissues: adult salivary gland. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    32.25 Larval Midgut    37.3 Larval Hindgut    84.2 Larval Malpighian Tubules    14.3 Larval Fat Body    86.4 Larval Salivary Gland    626.2 Larval Trachea    7.45 Larval Carcass    52.425 Adult Head    39.3 Adult Eye    8.575 Adult Brain    5 Adult Thoracic-Abdominal Ganglion    13.2 Adult Crop    23.8 Adult Midgut    23.9 Adult Hindgut    69.3 Adult Malpighian Tubules    18 Adult Fat Body    21.9 Adult Salivary Gland    484.9 Adult Heart    19.5 Adult VirginFemale Spermatheca    5.8 Adult InseminatedFemale Spermatheca    19.6 Adult Ovary    0.9 Adult Testis    19.4 Adult Male Accessory Gland    1.9 Adult Carcass    43.5 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    32.25 Larval Midgut    37.3 Larval Hindgut    84.2 Larval Malpighian Tubules    14.3 Larval Fat Body    86.4 Larval Salivary Gland  (626.2) Larval Trachea    7.45 Larval Carcass    52.425 Adult Head    39.3 Adult Eye    8.575 Adult Brain    5 Adult Thoracic-Abdominal Ganglion    13.2 Adult Crop    23.8 Adult Midgut    23.9 Adult Hindgut    69.3 Adult Malpighian Tubules    18 Adult Fat Body    21.9 Adult Salivary Gland    484.9 Adult Heart    19.5 Adult VirginFemale Spermatheca    5.8 Adult InseminatedFemale Spermatheca    19.6 Adult Ovary    0.9 Adult Testis    19.4 Adult Male Accessory Gland    1.9 Adult Carcass    43.5 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    32.25 Larval Midgut    37.3 Larval Hindgut    84.2 Larval Malpighian Tubules    14.3 Larval Fat Body    86.4 Larval Salivary Gland    626.2 Larval Trachea    7.45 Larval Carcass    52.425 Adult Head    39.3 Adult Eye    8.575 Adult Brain    5 Adult Thoracic-Abdominal Ganglion    13.2 Adult Crop    23.8 Adult Midgut    23.9 Adult Hindgut    69.3 Adult Malpighian Tubules    18 Adult Fat Body    21.9 Adult Salivary Gland    484.9 Adult Heart    19.5 Adult VirginFemale Spermatheca    5.8 Adult InseminatedFemale Spermatheca    19.6 Adult Ovary    0.9 Adult Testis    19.4 Adult Male Accessory Gland    1.9 Adult Carcass    43.5 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    32.25 Larval Midgut    37.3 Larval Hindgut    84.2 Larval Malpighian Tubules    14.3 Larval Fat Body    86.4 Larval Salivary Gland    626.2 Larval Trachea    7.45 Larval Carcass    52.425 Adult Head    39.3 Adult Eye    8.575 Adult Brain    5 Adult Thoracic-Abdominal Ganglion    13.2 Adult Crop    23.8 Adult Midgut    23.9 Adult Hindgut    69.3 Adult Malpighian Tubules    18 Adult Fat Body    21.9 Adult Salivary Gland    484.9 Adult Heart    19.5 Adult VirginFemale Spermatheca    5.8 Adult InseminatedFemale Spermatheca    19.6 Adult Ovary    0.9 Adult Testis    19.4 Adult Male Accessory Gland    1.9 Adult Carcass    43.5 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    32.25 Larval Midgut    37.3 Larval Hindgut    84.2 Larval Malpighian Tubules    14.3 Larval Fat Body    86.4 Larval Salivary Gland    626.2 Larval Trachea    7.45 Larval Carcass    52.425 Adult Head    39.3 Adult Eye    8.575 Adult Brain    5 Adult Thoracic-Abdominal Ganglion    13.2 Adult Crop    23.8 Adult Midgut    23.9 Adult Hindgut    69.3 Adult Malpighian Tubules    18 Adult Fat Body    21.9 Adult Salivary Gland    484.9 Adult Heart    19.5 Adult VirginFemale Spermatheca    5.8 Adult InseminatedFemale Spermatheca    19.6 Adult Ovary    0.9 Adult Testis    19.4 Adult Male Accessory Gland    1.9 Adult Carcass    43.5 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    32.25 Larval Midgut    37.3 Larval Hindgut    84.2 Larval Malpighian Tubules    14.3 Larval Fat Body    86.4 Larval Salivary Gland  626.2 Larval Trachea    7.45 Larval Carcass    52.425 Adult Head    39.3 Adult Eye    8.575 Adult Brain    5 Adult Thoracic-Abdominal Ganglion    13.2 Adult Crop    23.8 Adult Midgut    23.9 Adult Hindgut    69.3 Adult Malpighian Tubules    18 Adult Fat Body    21.9 Adult Salivary Gland    484.9 Adult Heart    19.5 Adult VirginFemale Spermatheca    5.8 Adult InseminatedFemale Spermatheca    19.6 Adult Ovary    0.9 Adult Testis    19.4 Adult Male Accessory Gland    1.9 Adult Carcass    43.5 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    32.25 Larval Midgut    37.3 Larval Hindgut    84.2 Larval Malpighian Tubules    14.3 Larval Fat Body    86.4 Larval Salivary Gland    626.2 Larval Trachea    7.45 Larval Carcass    52.425 Adult Head    39.3 Adult Eye    8.575 Adult Brain    5 Adult Thoracic-Abdominal Ganglion    13.2 Adult Crop    23.8 Adult Midgut    23.9 Adult Hindgut    69.3 Adult Malpighian Tubules    18 Adult Fat Body    21.9 Adult Salivary Gland    484.9 Adult Heart    19.5 Adult VirginFemale Spermatheca    5.8 Adult InseminatedFemale Spermatheca    19.6 Adult Ovary    0.9 Adult Testis    19.4 Adult Male Accessory Gland    1.9 Adult Carcass    43.5 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    32.25 Larval Midgut    37.3 Larval Hindgut    84.2 Larval Malpighian Tubules    14.3 Larval Fat Body    86.4 Larval Salivary Gland    626.2 Larval Trachea    7.45 Larval Carcass    52.425 Adult Head    39.3 Adult Eye    8.575 Adult Brain    5 Adult Thoracic-Abdominal Ganglion    13.2 Adult Crop    23.8 Adult Midgut    23.9 Adult Hindgut    69.3 Adult Malpighian Tubules    18 Adult Fat Body    21.9 Adult Salivary Gland    484.9 Adult Heart    19.5 Adult VirginFemale Spermatheca    5.8 Adult InseminatedFemale Spermatheca    19.6 Adult Ovary    0.9 Adult Testis    19.4 Adult Male Accessory Gland    1.9 Adult Carcass    43.5 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    39.3   NA  Eye    8.575   NA  Brain    5   32.25  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    13.2   NA  Crop    23.8   37.3  Midgut    23.9   84.2  Hindgut    69.3   14.3  Malpighian Tubules    no informative data   86.4  Fat Body    21.9   626.2  Salivary Gland    484.9   NA  Heart    19.5   7.45  Trachea    NA   NA  VirginFemale Spermatheca    5.8   NA  InseminatedFemale Spermatheca    19.6   NA  Ovary    0.9   NA  Testis    19.4   NA  Male Accessory Gland    1.9   52.425  Carcass    43.5 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    4 central nervous system, larvae L3    1 central nervous system, pupae P8    1 head, virgin 1-day female    1 head, virgin 4-day female    1 head, virgin 20-day female    2 head, mated 1-day female    1 head, mated 4-day female    1 head, mated 20-day female    2 head, mated 1-day male    1 head, mated 4-day male    1 head, mated 20-day male    1 salivary gland, larvae L3 wandering    5 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    1 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    2 fat body, pupae P8    1 carcass, larvae L3 wandering    4 carcass, 1-day adult    2 carcass, 4-day adult    2 carcass, 20-day adult    2 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    6 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    4 central nervous system, larvae L3    1 central nervous system, pupae P8    1 head, virgin 1-day female    1 head, virgin 4-day female    1 head, virgin 20-day female    2 head, mated 1-day female    1 head, mated 4-day female    1 head, mated 20-day female    2 head, mated 1-day male    1 head, mated 4-day male    1 head, mated 20-day male    1 salivary gland, larvae L3 wandering    5 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    1 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    2 fat body, pupae P8    1 carcass, larvae L3 wandering    4 carcass, 1-day adult    2 carcass, 4-day adult    2 carcass, 20-day adult    2 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    6 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    4 central nervous system, larvae L3    1 central nervous system, pupae P8    1 head, virgin 1-day female    1 head, virgin 4-day female    1 head, virgin 20-day female    2 head, mated 1-day female    1 head, mated 4-day female    1 head, mated 20-day female    2 head, mated 1-day male    1 head, mated 4-day male    1 head, mated 20-day male    1 salivary gland, larvae L3 wandering    5 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    1 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    2 fat body, pupae P8    1 carcass, larvae L3 wandering    4 carcass, 1-day adult    2 carcass, 4-day adult    2 carcass, 20-day adult    2 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    6 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    4 central nervous system, larvae L3    1 central nervous system, pupae P8    1 head, virgin 1-day female    1 head, virgin 4-day female    1 head, virgin 20-day female    2 head, mated 1-day female    1 head, mated 4-day female    1 head, mated 20-day female    2 head, mated 1-day male    1 head, mated 4-day male    1 head, mated 20-day male    1 salivary gland, larvae L3 wandering    5 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    1 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    2 fat body, pupae P8    1 carcass, larvae L3 wandering    4 carcass, 1-day adult    2 carcass, 4-day adult    2 carcass, 20-day adult    2 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    6 accessory gland, mated 4-day male    1 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    4 central nervous system, larvae L3    1 central nervous system, pupae P8    1 head, virgin 1-day female    1 head, virgin 4-day female    1 head, virgin 20-day female    2 head, mated 1-day female    1 head, mated 4-day female    1 head, mated 20-day female    2 head, mated 1-day male    1 head, mated 4-day male    1 head, mated 20-day male    1 salivary gland, larvae L3 wandering    5 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    1 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    2 fat body, pupae P8    1 carcass, larvae L3 wandering    4 carcass, 1-day adult    2 carcass, 4-day adult    2 carcass, 20-day adult    2 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    6 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    4 central nervous system, larvae L3    1 central nervous system, pupae P8    1 head, virgin 1-day female    1 head, virgin 4-day female    1 head, virgin 20-day female    2 head, mated 1-day female    1 head, mated 4-day female    1 head, mated 20-day female    2 head, mated 1-day male    1 head, mated 4-day male    1 head, mated 20-day male    1 salivary gland, larvae L3 wandering    5 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    1 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    2 fat body, pupae P8    1 carcass, larvae L3 wandering    4 carcass, 1-day adult    2 carcass, 4-day adult    2 carcass, 20-day adult    2 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    6 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    4 central nervous system, larvae L3    1 central nervous system, pupae P8    1 head, virgin 1-day female    1 head, virgin 4-day female    1 head, virgin 20-day female    2 head, mated 1-day female    1 head, mated 4-day female    1 head, mated 20-day female    2 head, mated 1-day male    1 head, mated 4-day male    1 head, mated 20-day male    1 salivary gland, larvae L3 wandering    5 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    1 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    2 fat body, pupae P8    1 carcass, larvae L3 wandering    4 carcass, 1-day adult    2 carcass, 4-day adult    2 carcass, 20-day adult    2 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    6 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    4 central nervous system, larvae L3    1 central nervous system, pupae P8    1 head, virgin 1-day female    1 head, virgin 4-day female    1 head, virgin 20-day female    2 head, mated 1-day female    1 head, mated 4-day female    1 head, mated 20-day female    2 head, mated 1-day male    1 head, mated 4-day male    1 head, mated 20-day male    1 salivary gland, larvae L3 wandering    5 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    1 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    2 fat body, pupae P8    1 carcass, larvae L3 wandering    4 carcass, 1-day adult    2 carcass, 4-day adult    2 carcass, 20-day adult    2 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    6 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderate expression to a trough of very low expression. Peak expression observed within 00-06 hour embryonic stages, during late pupal stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    12 embryo 04-06hr    17 embryo 06-08hr    1 embryo 08-10hr    2 embryo 10-12hr    5 embryo 12-14hr    4 embryo 14-16hr    2 embryo 16-18hr    2 embryo 18-20hr    2 embryo 20-22hr    2 embryo 22-24hr    2 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    5 larva L3 puffstage 7-9    4 white prepupae new    3 white prepupae 12hr    4 white prepupae 24hr    4 pupae 2d postWPP    6 pupae 3d postWPP    5 pupae 4d postWPP    2 adult male 01day    3 adult male 05day    4 adult male 30day    3 adult female 01day    1 adult female 05day    1 adult female 30day    1 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    12 embryo 04-06hr    17 embryo 06-08hr    1 embryo 08-10hr    2 embryo 10-12hr    5 embryo 12-14hr    4 embryo 14-16hr    2 embryo 16-18hr    2 embryo 18-20hr    2 embryo 20-22hr    2 embryo 22-24hr    2 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    5 larva L3 puffstage 7-9    4 white prepupae new    3 white prepupae 12hr    4 white prepupae 24hr    4 pupae 2d postWPP    6 pupae 3d postWPP    5 pupae 4d postWPP    2 adult male 01day    3 adult male 05day    4 adult male 30day    3 adult female 01day    1 adult female 05day    1 adult female 30day    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    12 embryo 04-06hr    17 embryo 06-08hr    1 embryo 08-10hr    2 embryo 10-12hr    5 embryo 12-14hr    4 embryo 14-16hr    2 embryo 16-18hr    2 embryo 18-20hr    2 embryo 20-22hr    2 embryo 22-24hr    2 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    5 larva L3 puffstage 7-9    4 white prepupae new    3 white prepupae 12hr    4 white prepupae 24hr    4 pupae 2d postWPP    6 pupae 3d postWPP    5 pupae 4d postWPP    2 adult male 01day    3 adult male 05day    4 adult male 30day    3 adult female 01day    1 adult female 05day    1 adult female 30day    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    12 embryo 04-06hr    17 embryo 06-08hr    1 embryo 08-10hr    2 embryo 10-12hr    5 embryo 12-14hr    4 embryo 14-16hr    2 embryo 16-18hr    2 embryo 18-20hr    2 embryo 20-22hr    2 embryo 22-24hr    2 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    5 larva L3 puffstage 7-9    4 white prepupae new    3 white prepupae 12hr    4 white prepupae 24hr    4 pupae 2d postWPP    6 pupae 3d postWPP    5 pupae 4d postWPP    2 adult male 01day    3 adult male 05day    4 adult male 30day    3 adult female 01day    1 adult female 05day    1 adult female 30day    1 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    12 embryo 04-06hr    17 embryo 06-08hr    1 embryo 08-10hr    2 embryo 10-12hr    5 embryo 12-14hr    4 embryo 14-16hr    2 embryo 16-18hr    2 embryo 18-20hr    2 embryo 20-22hr    2 embryo 22-24hr    2 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    5 larva L3 puffstage 7-9    4 white prepupae new    3 white prepupae 12hr    4 white prepupae 24hr    4 pupae 2d postWPP    6 pupae 3d postWPP    5 pupae 4d postWPP    2 adult male 01day    3 adult male 05day    4 adult male 30day    3 adult female 01day    1 adult female 05day    1 adult female 30day    1 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    12 embryo 04-06hr    17 embryo 06-08hr    1 embryo 08-10hr    2 embryo 10-12hr    5 embryo 12-14hr    4 embryo 14-16hr    2 embryo 16-18hr    2 embryo 18-20hr    2 embryo 20-22hr    2 embryo 22-24hr    2 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    5 larva L3 puffstage 7-9    4 white prepupae new    3 white prepupae 12hr    4 white prepupae 24hr    4 pupae 2d postWPP    6 pupae 3d postWPP    5 pupae 4d postWPP    2 adult male 01day    3 adult male 05day    4 adult male 30day    3 adult female 01day    1 adult female 05day    1 adult female 30day    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    12 embryo 04-06hr    17 embryo 06-08hr    1 embryo 08-10hr    2 embryo 10-12hr    5 embryo 12-14hr    4 embryo 14-16hr    2 embryo 16-18hr    2 embryo 18-20hr    2 embryo 20-22hr    2 embryo 22-24hr    2 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    5 larva L3 puffstage 7-9    4 white prepupae new    3 white prepupae 12hr    4 white prepupae 24hr    4 pupae 2d postWPP    6 pupae 3d postWPP    5 pupae 4d postWPP    2 adult male 01day    3 adult male 05day    4 adult male 30day    3 adult female 01day    1 adult female 05day    1 adult female 30day    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    12 embryo 04-06hr    17 embryo 06-08hr    1 embryo 08-10hr    2 embryo 10-12hr    5 embryo 12-14hr    4 embryo 14-16hr    2 embryo 16-18hr    2 embryo 18-20hr    2 embryo 20-22hr    2 embryo 22-24hr    2 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    5 larva L3 puffstage 7-9    4 white prepupae new    3 white prepupae 12hr    4 white prepupae 24hr    4 pupae 2d postWPP    6 pupae 3d postWPP    5 pupae 4d postWPP    2 adult male 01day    3 adult male 05day    4 adult male 30day    3 adult female 01day    1 adult female 05day    1 adult female 30day    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    1 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    1 embryonic S1    7 embryonic S3    0 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    0 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    3 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    3 tumorous blood cells mbn2    0 ovary fGS/OSS    0 ovary OSC    0 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    1 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    1 embryonic S1    7 embryonic S3    0 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    0 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    3 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    3 tumorous blood cells mbn2    0 ovary fGS/OSS    0 ovary OSC    0 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    1 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    1 embryonic S1    7 embryonic S3    0 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    0 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    3 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    3 tumorous blood cells mbn2    0 ovary fGS/OSS    0 ovary OSC    0 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    1 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    1 embryonic S1    7 embryonic S3    0 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    0 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    3 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    3 tumorous blood cells mbn2    0 ovary fGS/OSS    0 ovary OSC    0 ovary OSS    0 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    1 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    1 embryonic S1    7 embryonic S3    0 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    0 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    3 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    3 tumorous blood cells mbn2    0 ovary fGS/OSS    0 ovary OSC    0 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    1 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    1 embryonic S1    7 embryonic S3    0 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    0 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    3 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    3 tumorous blood cells mbn2    0 ovary fGS/OSS    0 ovary OSC    0 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    1 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    1 embryonic S1    7 embryonic S3    0 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    0 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    3 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    3 tumorous blood cells mbn2    0 ovary fGS/OSS    0 ovary OSC    0 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    1 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    1 embryonic S1    7 embryonic S3    0 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    0 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    3 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    3 tumorous blood cells mbn2    0 ovary fGS/OSS    0 ovary OSC    0 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    0 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    0 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    1 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    2 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    1 Caffeine 25 mg/ml 48 hrs, 4-day adult    0 Paraquat 5 mM 48 hrs, 4-day adult    2 Paraquat 10 mM 48 hrs, 4-day adult    3 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    0 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    0 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    1 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    2 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    1 Caffeine 25 mg/ml 48 hrs, 4-day adult    0 Paraquat 5 mM 48 hrs, 4-day adult    2 Paraquat 10 mM 48 hrs, 4-day adult    3 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    0 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    0 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    1 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    2 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    1 Caffeine 25 mg/ml 48 hrs, 4-day adult    0 Paraquat 5 mM 48 hrs, 4-day adult    2 Paraquat 10 mM 48 hrs, 4-day adult    3 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    0 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    0 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    1 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    2 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    1 Caffeine 25 mg/ml 48 hrs, 4-day adult    0 Paraquat 5 mM 48 hrs, 4-day adult    2 Paraquat 10 mM 48 hrs, 4-day adult    3 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    0 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    0 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    1 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    2 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    1 Caffeine 25 mg/ml 48 hrs, 4-day adult    0 Paraquat 5 mM 48 hrs, 4-day adult    2 Paraquat 10 mM 48 hrs, 4-day adult    3 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    0 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    0 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    1 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    2 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    1 Caffeine 25 mg/ml 48 hrs, 4-day adult    0 Paraquat 5 mM 48 hrs, 4-day adult    2 Paraquat 10 mM 48 hrs, 4-day adult    3 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    0 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    0 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    1 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    2 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    1 Caffeine 25 mg/ml 48 hrs, 4-day adult    0 Paraquat 5 mM 48 hrs, 4-day adult    2 Paraquat 10 mM 48 hrs, 4-day adult    3 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    0 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    0 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    1 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    2 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    1 Caffeine 25 mg/ml 48 hrs, 4-day adult    0 Paraquat 5 mM 48 hrs, 4-day adult    2 Paraquat 10 mM 48 hrs, 4-day adult    3 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_09 mE1_20_mRNA_expression_cluster_06
External Data & Images
Linkouts	BDGP expression data • PC00159 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0002931 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG11450-RA FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for net Stages(s) 4-6 Stages(s) 7-8 Stages(s) 9-10 Stages(s) 11-12
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | embryonic stage, with Scer\GAL4rho.PL netScer\UAS.cBa viable net107 net383 viable, with Scer\GAL4pnr-MD237 netGD4378 Other Phenotypes Allele visible net1 net3 net4 netCh86 netK netU87 netZ14 visible (with Df(2L)net18) netXR93 visible (with Df(2L)net62) net2-45 netB27 netCh86 netZ14 visible (with netB27) netunspecified visible (with netunspecified) netB27 netZ14 visible (with netZ14) netunspecified visible | dominant net41e4 visible | recessive net1 net383 netB27 netK1 netunspecified visible | semidominant netCh86 wild-type net+tRIIb Phenotype manifest in Allele 1st posterior cell netCh86 1st posterior cell (with Df(2L)net62) net2-45 netCh86 2nd posterior cell netCh86 3rd posterior cell netB27 netCh86 netU87 netZ14 3rd posterior cell (with Df(2L)net62) netB27 netZ14 3rd posterior cell (with netB27) netunspecified 3rd posterior cell (with netunspecified) netB27 netZ14 3rd posterior cell (with netZ14) netunspecified marginal cell netCh86 marginal cell | distal (with Df(2L)net62) netB27 posterior cell net1 submarginal cell net1 netCh86 submarginal cell | distal (with Df(2L)net62) netB27 wing net3 net4 net41e4 netB27 netK netZ14 netunspecified wing, with Scer\GAL4Bx-MS1096 netScer\UAS.cBa wing (with Df(2L)net62) net2-45 netCh86 wing vein net1 net2-45 net3 net383 net41e4 netCh86 netK netZ14 wing vein, with Scer\GAL4Bx-MS1096 netScer\UAS.cBa wing vein, with Scer\GAL4nub-AC-62 netScer\UAS.cBa wing vein (with Df(2L)net62) net2-45 netCh86 wing vein (with net2-45) netCh86 wing vein (with netCh86) net2-45 wing vein | ectopic net1 net383 net4 netB27 netCh86 netK1 netU87 netZ14 netunspecified wing vein | ectopic (with Df(2L)net18) netXR93 wing vein | ectopic (with Df(2L)net62) net2-45 netB27 netCh86 netZ14 wing vein | ectopic (with net2-45) netCh86 wing vein | ectopic (with netB27) netunspecified wing vein | ectopic (with netCh86) net2-45 wing vein | ectopic (with netunspecified) netB27 netZ14 wing vein | ectopic (with netZ14) netunspecified wing vein L3, with Scer\GAL4Bx-MS1096 netScer\UAS.cBa wing vein L4, with Scer\GAL4Bx-MS1096 netScer\UAS.cBa wing vein L5, with Scer\GAL4Bx-MS1096 netScer\UAS.cBa
Classical Alleles ( 29 )
For All Classical Alleles Show
Allele of net Class Mutagen Stocks Known lesion net1 loss of function allele spontaneous 21 Yes netf04249 piggyBac transposase 3 -- net11c ethyl nitrosourea 1 -- nete01104 piggyBac transposase 1 -- netMB09620 1 -- net107 ethyl methanesulfonate 0 -- net18 X ray 0 -- net2-45 0 -- net2 spontaneous 0 -- net383 X ray 0 -- net38j spontaneous 0 -- net3 spontaneous 0 -- net41e4 spontaneous 0 -- net4 spontaneous X ray 0 -- net9P39.6 ethyl methanesulfonate 0 -- netB27 natural population 0 -- netCh86 natural population 0 -- netD90 ethyl methanesulfonate 0 -- nete01122 piggyBac transposase 0 -- netF170 ethyl methanesulfonate 0 -- netK1 loss of function allele spontaneous 0 Yes netK7 0 -- netK 0 -- netKP92 0 -- netPM50 P-element activity 0 Yes netU87 natural population 0 -- netunspecified 0 -- netXR93 loss of function allele X ray 0 Yes netZ14 natural population 0 --
Alleles Carried on Transgenic Constructs ( 8 )
For All Alleles Carried on Transgenic Constructs Show
Allele of net Class Mutagen Stocks Known lesion netGD4378 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes netJF02102 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes net+tRI in vitro construct - genomic fragment 0 Yes net+tRIIa in vitro construct - genomic fragment 0 Yes net+tRIIb in vitro construct - genomic fragment 0 Yes netKK100701 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes netNIG.11450R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes netScer\UAS.cBa in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2L)Exel6001 (Ryder, 2004.4.26) Df(2L)net18 (Brentrup et al., 2000, Korochkina and Golubovsky, 1978) Df(2L)net62 (LaJeunesse et al., 2001, Korochkina and Golubovsky, 1978, Opper et al., 1987, Woodhouse et al., 1994) Df(2L)net107 (LaJeunesse et al., 2001) Df(2L)net-PM47C (LaJeunesse et al., 2001) Df(2L)net-PMF (Caggese et al., 1994) Df(2L)PM1 (Caggese et al., 1988, Caggese et al., 1994) Df(2L)PM4 (Brentrup et al., 2000) Df(2L)PM44 (Caggese et al., 1988, Caggese et al., 1994) Df(2L)PM45 (Caggese et al., 1988) Df(2L)PM51 (Caggese et al., 1988) Df(2L)PM59 (Caggese et al., 1988, Caggese et al., 1994) Df(2L)PM73 (Caggese et al., 1988) Df(2L)PM82 (Caggese et al., 1988) Df(2L)PM91 (Caggese et al., 1988) Df(2L)PMD (Caggese et al., 1994) Df(2L)PMG (Caggese et al., 1988, Caggese et al., 1994) Df(2L)TE21A (Caggese et al., 1988, Caggese et al., 1994) In(2L)TE99(Z)XR93 (Brentrup et al., 2000) Ts(2Rt;4Rt)astv
Not disrupted in	Df(2L)al (Korochkina and Golubovsky, 1978, Lewis, 1945) Df(2L)S1 (Lewis, 1945) Df(2L)S2 (Lewis, 1945) Df(2L)S3 (Lewis, 1945) Df(2L)S4 (Lewis, 1945) Df(2L)S5 (Lewis, 1945) Df(2L)U314 (Brentrup et al., 2000) Ts(1Lt;2Rt)Hwbap
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD4378} NA P{KK100701} NA P{NIG.11450R} NA P{TRiP.JF02102} NA P{UAS-net.B} NA characterization construct P{net.RI} NA P{net.RIIa} NA P{net.RIIb} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions P{}netPM50 NA PBac{RB}nete01104 NA PBac{RB}nete01122 NA PBac{WH}netf04249 NA roo{}netK1 NA insertion of enhancer trap binary system Mi{ET1}netMB09620 No
Gene Ontology: Function, Process & Cellular Component ( 7 unique terms )
Terms Based on Experimental Evidence ( 2 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
imaginal disc-derived wing vein specification inferred from mutant phenotype (Brentrup et al., 2000) negative regulation of transcription from RNA polymerase II promoter inferred from mutant phenotype (Brentrup et al., 2000)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 5 terms )
Molecular Function
CV term Evidence References
protein dimerization activity inferred from electronic annotation with InterPro:IPR011598 (FlyBase Curators et al., 2004-) sequence-specific DNA binding transcription factor activity inferred from sequence or structural similarity with HLH54F (Peyrefitte et al., 2001)
Biological Process
CV term Evidence References
imaginal disc-derived wing vein morphogenesis traceable author statement (de Celis, 2003) regulation of transcription, DNA-dependent inferred from sequence or structural similarity with HLH54F (Peyrefitte et al., 2001)
Cellular Component
CV term Evidence References
nucleus non-traceable author statement (Brentrup, 2000.9.6)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions net allele Gene References net1 emc (Baonza and Garcia-Bellido, 1999) H (Johannes and Preiss, 2002) mor (Marenda et al., 2004) nmo (Verheyen et al., 2001) osa (Marenda et al., 2004) Rpd3 (Marenda et al., 2004) Snr1 (Marenda et al., 2004) net9P39.6 Dl (Klein and Campos-Ortega, 1992) net107 Mer (LaJeunesse et al., 2001) net383 Mer (LaJeunesse et al., 2001) netD90 Dl (Klein and Campos-Ortega, 1992) netF170 Dl (Klein and Campos-Ortega, 1992) netKP92 Dl (Klein and Campos-Ortega, 1992) netScer\UAS.cBa rho (Brentrup et al., 2000) netunspecified ast (Lewis, 1945) Egfr (Diaz-Benjumea and Garcia-Bellido, 1990) S (Lewis, 1945) unspecified ast   aya (Suzuki et al., 2000) csw (Firth et al., 2000) rho (Sturtevant and Bier, 1995)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 69604 DroID - A comprehensive database of gene and protein interactions. • FBgn0002931 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 45339
Orthologs
OrthoDB Orthologs (36) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6TQMW3 Dipteran inclusive ortholog search EOG605S8V Insect inclusive ortholog search EOG66M9FB Arthropod inclusive ortholog search EOG69ZW50 Metazoa inclusive ortholog search EOG6064TX
Orthologs in Drosophila Species (EOG6TQMW3)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\net     Drosophila simulans   Dsim\GD22993 Y   Drosophila sechellia   Dsec\GM16704 Y   Drosophila erecta   Dere\GG24687 Y   Drosophila yakuba   Dyak\GE16494 Y   Drosophila ananassae   Dana\GF14859 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA11011 Y   Drosophila persimilis   Dper\GL13900 Y   Drosophila willistoni   Dwil\GK21091 Y   Drosophila virilis   Dvir\GJ16214 Y   Drosophila mojavensis   Dmoj\GI16429 Y   Drosophila grimshawi   Dgri\GH10058 Y
Orthologs in non-Drosophila Dipterans (EOG605S8V)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL008190   Anopheles gambiae Malaria mosquito  Agam\AGAP009983   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ003113
Orthologs in non-Dipteran Insects (EOG66M9FB)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB46156   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH20278   Atta cephalotes Leafcutter ant  Acep\ACEP23825   Camponotus floridanus Florida carpenter ant  Cflo\CFLO17902   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL22756   Linepithema humile Argentine ant  Lhum\LH13719   Pogonomyrmex barbatus Red harvester ant  Pbar\PB18555   Solenopsis invicta Red fire ant  Sinv\SINV15468   Acyrthosiphon pisum Pea aphid  Apim\ACYPI21611   Bombyx mori Silkmoth  Bmor\BGIBMGA009116   Pediculus humanus Human body louse  Phum\PHUM129210   Tribolium castaneum Red flour beetle  Tcas\TC005579
Orthologs in non-Insect Arthropods (EOG69ZW50)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\JGI_V11_48661   Ixodes scapularis Deer tick  Isca\ISCW011969
Orthologs in non-Arthropod Metazoa (EOG6064TX)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_017983gn   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_011315gn   Danio rerio Zebrafish  Drer\atoh8   Gallus gallus Domestic chicken  Ggal\ATOH8   Mus musculus House mouse  Mmus\Atoh8   Rattus norvegicus Norway rat  Rnor\Atoh8   Homo sapiens Human  Hsap\ATOH8
Human Orthologs (1)
Gene OMIM HGNC Hsap\ATOH8
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD22993 Drosophila sechellia Dsec\GM16704 Drosophila erecta Dere\GG24687 Drosophila yakuba Dyak\GE16494 Drosophila ananassae Dana\GF14859 Drosophila pseudoobscura pseudoobscura Dpse\GA11011 Drosophila persimilis Dper\GL13900 Drosophila willistoni Dwil\GK21091 Drosophila virilis Dvir\GJ16214 Drosophila mojavensis Dmoj\GI16429 Drosophila grimshawi Dgri\GH10058
Stocks & Reagents
Stocks Listed in FlyBase ( 29 )
Bloomington	354 net1 26489 w1118; Mi{ET1}netMB09620 8470 Df(2L)BSC51, net1 cn1/SM5 8471 Df(2L)BSC52, net1 cn1/SM5 5491 net1 cn1 Hsf1/CyO
Harvard	- PBac{RB}nete01104 - PBac{WH}netf04249
Kyoto	105868 net1 105870 net1 ed1 Su(dx)2 108257 net1 cn1 Hsf1/CyO 108848 Df(2L)BSC16, net1 cn1/SM6a
VDRC	v41069 w1118; P{GD4378}v41069
	More stocks available...
Genomic Clones ( 1 )
	BACR09J20 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 51 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	IP14510
Other clones	FI03708 FI10001 IP14710
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GH17202
Other clones	73393 173723 CK01.36C.E4 EK031150 EK142759 EK156341 EK186851 EK202929 EK221222 EK240701 EK278137 EK290319 EK306150 EP14850 IP14410 IP14610 RP004110862 RP004143467 RP004186272 RP004187237 RP004191188 RP004194019 RP004208290 RP004211057 RP004212144 RP004227191 RP004231202 RP004238630 RP004239439 RP004248749 RP004249337 RP004257565 RP004358429 RP004372926 RP004406700 RP004408151 RP004454262 RP004461725 RP004507539 RP004510171 RP004518350 RP004522087 RP004523245 RP004541199 RP004546403 RP004552377
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0002931 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 45339
Antibody Information
Other Information
Discoverer
Etymology
	The expression pattern of CG11450 overlaps with that of the bHLH transcription factor twist (twi), so the gene was named "shout" after "Twist and Shout" by Lennon and McCartney (1963). (Moore et al., 2000)
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: shout CG11450 (Moore et al., 2000)
Source for database merge of	Source for merge of: net shout (FlyBase, 1996-)
Additional comments	Source for merge of net shout was sequence comparison (date:010307). (FlyBase, 1996-)
Other Comments
	net exhibits a two tiered control in wing vein versus intervein fate by repressing rho transcription and interfering with Egfr signalling downstream of rho. (Brentrup et al., 2000) Egfr signalling and net exert mutually antagonising activities during the specification of wing vein versus intervein fate. net is required to maintain intervein development by restricting Egfr signalling to the net-free vein regions of the wing disc. (Brentrup et al., 2000) Extra vein mutations cause enhancement of rhohs.PSt ectopic vein phenotype and suppression of the rhove-1 loss of vein phenotype. (Sturtevant and Bier, 1995) Mutants display hyperplastic phenotype, showing tissue overgrowth in mitotic recombination clones. (Mechler, 1994) A screen that identified Dominant enhancers of the wing phenotype associated with Dl9P and DlFE32 identified 4 new net alleles. (Klein and Campos-Ortega, 1992) Mutations in net cause extra longitudinal or transverse veins with plexated vein phenotypes. Px, px, net, dsr and emc belong to the same synergistic group and act synergistically against kn, fu and shf. (Diaz-Benjumea and Garcia-Bellido, 1990) Px, net, dsr, px and emc loci belong to the plexus phenotypic group within the 'excess-of-vein' mutant class. Extra veins occur parallel to and between normal veins that connect to each other by crossveins, forming plexi. Plexi are distal. Proximal regions shift to the margin leaving a central area devoid of veins. Sensilla and chaetae appear shifted. Combinations of mutations produce superadditive phenotypes. (Diaz-Benjumea et al., 1989)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_080081 NM_001272860 RefSeq (Proteins) • NP_524820 NP_001259789 DDBJ / EMBL / GenBank • AAF51562 AAK14073 AF303350 AGB92326 CZ478919 CZ478940 Entrez Gene - A searchable database of RefSeq genes. • 45339 UniProtKB/TrEMBL • Q7KH24 Q9VPI8
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Myc-type, basic helix-loop-helix (bHLH) domain (IPR011598) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • PC00159
Linkouts
	BioGRID - A database of protein and genetic interactions • 69604 DroID - A comprehensive database of gene and protein interactions. • FBgn0002931 DRSC - Results from RNAi screens. • FBgn0002931 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0002931 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG11450-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0002931 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 45339 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /gene/net1.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 45339 modMine - Data generated by the modENCODE project. • FBgn0002931
Synonyms & Secondary IDs ( 6 )
Reported As
Symbol Synonym	bHLHc42 (Stevens et al., 2008) CG11450 (Giannakou and Dow, 2001, Peyrefitte et al., 2001, Ledent and Vervoort, 2001, Moore et al., 2000, Stevens et al., 2008, Stevens et al., 2008, Perkins et al., 2009.3.31, Keleman et al., 2009.8.5, Muse et al., 2007, Chang et al., 2008, Seinen et al., 2010) Group IId (LaJeunesse et al., 2001) net (Weisman, 2003, Terriente Felix and de Celis, 2008.1.16, Kankel et al., 2007, Choksi et al., 2006, Chang et al., 2008, Angulo et al., 2004, Terriente-Félix and de Celis, 2009, Muse et al., 2007, Muse et al., 2007, Bina et al., 2010, Terriente-Félix et al., 2010, Rendina et al., 2010, Beltran et al., 2007, Japanese National Institute of Genetics, 2012.5.21, Zhou et al., 2012) Shout (Peyrefitte et al., 2001) shout
Name Synonym	net (Chang et al., 2008)
Secondary FlyBase IDs
	FBgn0031215
References ( 93 )
Generate a list of	All references relating to this gene ( 93 )
List References by type	Research paper  ( 54 ) Supplementary material  ( 5 ) Review  ( 6 ) Personal communication to FlyBase  ( 11 ) Abstract  ( 9 ) FlyBase analysis  ( 1 ) Stock list  ( 2 ) DNA/RNA sequence record  ( 1 ) Website  ( 1 ) Book  ( 2 ) Curated genome annotation  ( 1 )
Recent research papers ( 1 )
	Zhou et al., 2012, PLoS Genet. 8(3): e1002593 Phenotypic plasticity of the Drosophila transcriptome. [FBrf0217974] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...