FlyBase Gene Report: Dmel\ng1

General Information

Symbol

Dmel\ng1

Species

D. melanogaster

Name

new glue 1

Annotation Symbol

CG10781

Feature Type

protein_coding_gene

FlyBase ID

FBgn0002933

Gene Model Status

Current

Stock Availability

2 publicly available

Gene Summary

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Interactive Fly

Also Known As

ng-1, SC1, ng, ngp

Key Links

Genomic Location

Cytogenetic map

3C10-3C10

Sequence location

X:3,242,412..3,242,934 [+]

Recombination map

1-2

RefSeq locus

NC_004354 REGION:3242412..3242934

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (0 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Cellular Component (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Gene Group (FlyBase)

Protein Family (UniProt)

Protein Signatures (InterPro)

New-glue 1-3-like

Summaries

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\ng1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry P23938)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Supported by strand-specific RNA-Seq data.

Gene model reviewed during 5.46

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

ng1-RA

FBtr0070523

NM_057307

523

107

Additional Transcript Data and Comments

Reported size (kB)

0.6 (northern blot)

(Furia et al., 1990)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

ng1-PA

FBpp0070499

11.4

107

12.21

P23938

NP_476655

AAF45856

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

107 (aa); 11.3 (kD predicted)

(Furia et al., 1990)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

New-glue 1-3-like

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

P23938

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\ng1 using the Feature Mapper tool.

External Data

Crossreferences

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.21

Transcript Expression

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

(Furia et al., 1990)

Additional Descriptive Data

The expression of the ng1 transcript peaks at the onset of third larval instar, and persists through the rest of third larval instar.

(Furia et al., 1990)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\ng1 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\ng1

View exonic expression by tissue for Dmel\ng1

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

31300/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0002933

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0002933

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0002933

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG10781

Flygut - An atlas of the Drosophila adult midgut

FBgn0002933

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 0 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 8 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of ng1

Transgenic constructs containing regulatory region of ng1

Aberrations (Deficiencies and Duplications) ( 2 )

Inferred from experimentation ( 2 )

Gene disrupted in

Df(1)N^23-24c

(Furia et al., 1993)

Gene not disrupted in

Df(1)N-54l9

(Furia et al., 1993)

Inferred from location ( 22 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (0)

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (0)

Mus musculus (laboratory mouse) (0)

Xenopus tropicalis (Western clawed frog) (0)

Danio rerio (Zebrafish) (0)

Caenorhabditis elegans (Nematode, roundworm) (0)

Anopheles gambiae (African malaria mosquito) (0)

Arabidopsis thaliana (thale-cress) (0)

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:ng1. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (1)

ng2

2 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Other Interaction Browsers

Summary of Genetic Interactions

esyN Network Diagram

Other Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

57829

DroID - A comprehensive database of gene and protein interactions.

FBgn0002933

MIST (protein-protein) - An integrated Molecular Interaction Database

31300

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

1-2

Cytogenetic map

3C10-3C10

Sequence location

X:3,242,412..3,242,934 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

3C10-3C10

Limits computationally determined from genome sequence between P{EP}dnc^EP1395 and P{EP}dnc^EP973

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

1-2

(FlyBase, 2016)

1-2.7

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (2)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

60066

y¹ sc^* v¹ sev²¹; P{TRiP.HMC05059}attP40

VDRC

v109143

P{KK115882}VIE-260B

Genomic Clones (10)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (1)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0002933

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0002933

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

The binding properties of the ng-EcRE, a directly repeated half site (DR) located within the coding region of ng1, are studied to investigate the properties of EcREs composed of DRs. The ng-EcRE contacts the EcR/usp heterodimer through the DRs spaced by 12bp, this element may interact efficiently with Hr38 and Hr39. The ng-EcRE can promote functional interactions in vitro as well as in vivo, acting as a transcriptional enhancer able to confer a specific developmental expression profile to a minimal promoter in transgenic flies.

(Crispi et al., 1998)

An ecdysone-responsive element is required for high level expression of the ng1 intermoult gene.

(D'Avino et al., 1995)

ng1 RNA expression has been analysed in wild-type and br mutant larvae.

(D'Avino et al., 1995)

DNA-blotting assay has identified a high affinity ecdysone receptor binding site within the ng1 coding sequence. EMSA assay demonstrates the 93bp 'ng element' is able to bind an EcR/usp heterodimer and usp alone.

(D'Avino et al., 1995)

ng4 and ng1 originate by means of a gene duplication event. Also a strong similarity exists between ng1 and ng2 5' flanking regions.

(Furia et al., 1993)

An investigation of the relationship between transcription, puffing and hormone regulation of intermoult puff was analysed using ecd¹ mutant embryos: ng1 expression is totally unaffected by shifting ecd¹ embryos to the restrictive temperature, 30^oC.

(Furia et al., 1992)

Relationship to Other Genes

Source for database merge of

Additional comments

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (12)

Reported As

Symbol Synonym

CG10781

(Hosono et al., 2015, Chen et al., 2012, Zhang et al., 2010, Keleman et al., 2009.8.5, Perkins et al., 2009)

EG:96G10.5

SC1

(Furia et al., 1990)

(Buszczak and Segraves, 1998, Wang et al., 1998)

ng-1

(McBrayer et al., 2007, Yin et al., 2007, Kotlikova et al., 2006, Gibson et al., 2005, Xu et al., 2002, Crispi et al., 2001, Crispi et al., 1998, Fisk and Thummel, 1998, Furia et al., 1998, Hall and Thummel, 1998, Crispi et al., 1995, D'Avino et al., 1995, D'Avino et al., 1995, D'Avino et al., 1995, Nighorn et al., 1994, Furia et al., 1993, Furia et al., 1992)

ng1

(Chafino et al., 2023, Zhai et al., 2012, Sharma et al., 2011, Kuan et al., 2009, Liu and Lehmann, 2008, Jensen et al., 2007, Reiter et al., 2007)

ngp

(Furia et al., 1990)

Name Synonyms

New glue1

(Li et al., 2022)

nested

(Furia, 1990.3.14)

new glue

(Yin et al., 2007)

new glue 1

new glue protein

Secondary FlyBase IDs

FBgn0010372

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 27 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

31300

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

P23938

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P23938

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

P23938

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

31300/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0002933

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0002933

FlyMine - An integrated database for Drosophila genomics

FBgn0002933

InterPro - A database of protein families, domains and functional sites

New-glue 1-3-like

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG10781

MARRVEL_MODEL - MARRVEL (model organism gene)

31300

Linkouts

BioGRID - A database of protein and genetic interactions.

57829

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0002933

DroID - A comprehensive database of gene and protein interactions.

FBgn0002933

DRSC - Results frm RNAi screens

FBgn0002933

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0002933

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0002933

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG10781

Flygut - An atlas of the Drosophila adult midgut

FBgn0002933

MIST (protein-protein) - An integrated Molecular Interaction Database

31300

References (52)

The following external sites may use different assemblies or annotations than FlyBase.

-0.21

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