• Home
• Tools
  • Tools Overview
  • Query Tools

    
    • QuickSearch
    • QueryBuilder
    • TermLink
    • CytoSearch
    • RNA-Seq Search
    • Interactions Browser
    • ImageBrowse
    • Find A Person
    • Google™ FlyBase
  • Genomic/Map Tools

    
    • BLAST
    • GBrowse
    • GBrowse 2 Beta
    • CytoSearch
    • Feature Mapper
    • Aberrations Maps
    • Chromosome Maps
    • Coordinates Converter
  • Retrieve/Convert

    
    • Batch Download
    • Coordinates Converter
    • ID Converter
  • Data Submission

    
    • Fast-Track Your Paper
    • Submit Personal Comm
    • Add A New Person
    • Update Person Info
  • People

    
    • Find A Person
    • Add A New Person
    • Update Person Info
• Files
  • Files Overview
  • Current release
  • Archived releases
  • Map Conversion
  • FTP site:
  • Releases (FTP)
  • Genomes (FTP)
• Species
  • Phylogeny
  • Sequenced Species
  • Synteny Table
  • Color Illustrations
  • Abbreviations
• Documents
  • About FlyBase
  • Reference Manual:
  • Sections
  • Contents
  • Nomenclature
  • Release Notes
• Resources
  • All Resources
  • Model Organisms
  • Stock Collections
  • Vectors & Constructs
  • Other links:
  • BDGP
  • DGRC
  • DIS by issue
  • FlyExpress
  • Interactive Fly
  • modENCODE
  • Textpresso for Fly
• News
  • News
  • FlyBase Forum
  • FlyBase Positions
  • Meetings
  • Courses
  • Fly Board
  • White papers
  • Funding
  • bionet.dros
• Help
  • Google™ FlyBase
  • Site Map
  • FAQs
  • FlyBase Guides
  • Video Tutorials
  • Report help
  • New to Flies
  • Pers. comm.
  • Author guidelines
  • Contact FlyBase
• Archives
  • Prior Release
  • Other Archives
  • Coordinate Converter

Gene Dmel\ng1

General Information
Symbol	Dmel\ng1	Species	D. melanogaster
Name	new glue 1	Annotation symbol	CG10781
Feature type	protein_coding_gene	FlyBase ID	FBgn0002933
Gene Model Status	Current	Stock availability	None publicly available
Also Known As	ng-1, ng
Genomic Location
Chromosome (arm)	X	Recombination map
Cytogenetic map	3C10-3C10	Sequence location	X:3,136,445..3,136,967 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene new glue 1 is referred to in FlyBase by the symbol Dmel\ng1 (CG10781, FBgn0002933). It is a protein_coding_gene from Drosophila melanogaster. It is reported to have molecular function: structural molecule activity. It is reported to be involved in the biological process: puparial adhesion. 6 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of no expression detected. Peak expression observed during late larval stages. Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: larval salivary gland. Comments on Affy2 ProbeSet: ProbeSet 1624534_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of ng1. Gene sequence location is X:3136445..3136967.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 3C10-3C10   Limits computationally determined from genome sequence between P{EP}dncEP1395 and P{EP}dncEP973
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\ng1 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Supported by strand-specific RNA-Seq data. Gene model reviewed during 5.46 DGC clone RH11838 appears problematic: incomplete CDS
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) ng1-RA FBtr0070523  NM_057307   523   107
Additional Transcript Data & Comments
Reported size (kB)	0.6 (northern blot) (Furia et al., 1990)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein ng1-PA   FBpp0070499   11.4   107   12.21     NP_476655   AAF45856
Additional Polypeptide Data & Comments
Reported size (kDa)	107 (aa); 11.3 (kD predicted) (Furia et al., 1990)
Comments
External Data
Linkouts
Crossreferences
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE014298 AAF45856   AL024484 CAA19667   AY113385   BI607868   EL869641   EL869642   EL869643   EL869644   X52118 CAA36363
UniProtKB/Swiss-Prot	P23938
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References protein binding site FBsf0000159937 X:3,136,723..3,136,747 bound_moiety=EcR-XP comment=putative Ecdysone Response Element (EcRE) composed of two directly repeated half-sites 5'-AGGTCA-3' separated by 12 bp; there may be a third degenerate repeat in the 12 bp; bound by Hr38 protein alone or by EcR- usp and Hr38-usp protein complexes evidence=experimental (Crispi et al., 1998, D'Avino et al., 1995) regulatory region FBsf0000435154 X:3,136,683..3,136,771 evidence=Reporter construct (in vivo) (Crispi et al., 1998, Halfon, 2012.6.19) regulatory region FBsf0000436678 X:3,136,176..3,136,299 evidence=Reporter construct (in vivo) (Crispi et al., 2001, Halfon, 2012.6.19)
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
northern blot Stage Tissue/Position (including subcellular localization) Reference third instar larval stage     (Furia et al., 1990)
Additional Descriptive Data
	The expression of the ng1 transcript peaks at the onset of third larval instar, and persists through the rest of third larval instar. (Furia et al., 1990)
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: larval salivary gland. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    3.2 Larval Midgut    1 Larval Hindgut    0.8 Larval Malpighian Tubules    2.2 Larval Fat Body    3.6 Larval Salivary Gland    9537.6 Larval Trachea    11.225 Larval Carcass    4.9 Adult Head    3.4 Adult Eye    2.425 Adult Brain    2.4 Adult Thoracic-Abdominal Ganglion    2.8 Adult Crop    3 Adult Midgut    3.1 Adult Hindgut    3.3 Adult Malpighian Tubules    2.5 Adult Fat Body    5.9 Adult Salivary Gland    2.7 Adult Heart    0.5 Adult VirginFemale Spermatheca    4.1 Adult InseminatedFemale Spermatheca    2.1 Adult Ovary    1.5 Adult Testis    1.9 Adult Male Accessory Gland    1.7 Adult Carcass    2.1 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    3.2 Larval Midgut    1 Larval Hindgut    0.8 Larval Malpighian Tubules    2.2 Larval Fat Body    3.6 Larval Salivary Gland  (9537.6) Larval Trachea    11.225 Larval Carcass    4.9 Adult Head    3.4 Adult Eye    2.425 Adult Brain    2.4 Adult Thoracic-Abdominal Ganglion    2.8 Adult Crop    3 Adult Midgut    3.1 Adult Hindgut    3.3 Adult Malpighian Tubules    2.5 Adult Fat Body    5.9 Adult Salivary Gland    2.7 Adult Heart    0.5 Adult VirginFemale Spermatheca    4.1 Adult InseminatedFemale Spermatheca    2.1 Adult Ovary    1.5 Adult Testis    1.9 Adult Male Accessory Gland    1.7 Adult Carcass    2.1 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    3.2 Larval Midgut    1 Larval Hindgut    0.8 Larval Malpighian Tubules    2.2 Larval Fat Body    3.6 Larval Salivary Gland  (9537.6) Larval Trachea    11.225 Larval Carcass    4.9 Adult Head    3.4 Adult Eye    2.425 Adult Brain    2.4 Adult Thoracic-Abdominal Ganglion    2.8 Adult Crop    3 Adult Midgut    3.1 Adult Hindgut    3.3 Adult Malpighian Tubules    2.5 Adult Fat Body    5.9 Adult Salivary Gland    2.7 Adult Heart    0.5 Adult VirginFemale Spermatheca    4.1 Adult InseminatedFemale Spermatheca    2.1 Adult Ovary    1.5 Adult Testis    1.9 Adult Male Accessory Gland    1.7 Adult Carcass    2.1 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    3.2 Larval Midgut    1 Larval Hindgut    0.8 Larval Malpighian Tubules    2.2 Larval Fat Body    3.6 Larval Salivary Gland    9537.6 Larval Trachea    11.225 Larval Carcass    4.9 Adult Head    3.4 Adult Eye    2.425 Adult Brain    2.4 Adult Thoracic-Abdominal Ganglion    2.8 Adult Crop    3 Adult Midgut    3.1 Adult Hindgut    3.3 Adult Malpighian Tubules    2.5 Adult Fat Body    5.9 Adult Salivary Gland    2.7 Adult Heart    0.5 Adult VirginFemale Spermatheca    4.1 Adult InseminatedFemale Spermatheca    2.1 Adult Ovary    1.5 Adult Testis    1.9 Adult Male Accessory Gland    1.7 Adult Carcass    2.1 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    3.2 Larval Midgut    1 Larval Hindgut    0.8 Larval Malpighian Tubules    2.2 Larval Fat Body    3.6 Larval Salivary Gland    9537.6 Larval Trachea    11.225 Larval Carcass    4.9 Adult Head    3.4 Adult Eye    2.425 Adult Brain    2.4 Adult Thoracic-Abdominal Ganglion    2.8 Adult Crop    3 Adult Midgut    3.1 Adult Hindgut    3.3 Adult Malpighian Tubules    2.5 Adult Fat Body    5.9 Adult Salivary Gland    2.7 Adult Heart    0.5 Adult VirginFemale Spermatheca    4.1 Adult InseminatedFemale Spermatheca    2.1 Adult Ovary    1.5 Adult Testis    1.9 Adult Male Accessory Gland    1.7 Adult Carcass    2.1 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    3.2 Larval Midgut    1 Larval Hindgut    0.8 Larval Malpighian Tubules    2.2 Larval Fat Body    3.6 Larval Salivary Gland  (9537.6) Larval Trachea    11.225 Larval Carcass    4.9 Adult Head    3.4 Adult Eye    2.425 Adult Brain    2.4 Adult Thoracic-Abdominal Ganglion    2.8 Adult Crop    3 Adult Midgut    3.1 Adult Hindgut    3.3 Adult Malpighian Tubules    2.5 Adult Fat Body    5.9 Adult Salivary Gland    2.7 Adult Heart    0.5 Adult VirginFemale Spermatheca    4.1 Adult InseminatedFemale Spermatheca    2.1 Adult Ovary    1.5 Adult Testis    1.9 Adult Male Accessory Gland    1.7 Adult Carcass    2.1 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    3.2 Larval Midgut    1 Larval Hindgut    0.8 Larval Malpighian Tubules    2.2 Larval Fat Body    3.6 Larval Salivary Gland  (9537.6) Larval Trachea    11.225 Larval Carcass    4.9 Adult Head    3.4 Adult Eye    2.425 Adult Brain    2.4 Adult Thoracic-Abdominal Ganglion    2.8 Adult Crop    3 Adult Midgut    3.1 Adult Hindgut    3.3 Adult Malpighian Tubules    2.5 Adult Fat Body    5.9 Adult Salivary Gland    2.7 Adult Heart    0.5 Adult VirginFemale Spermatheca    4.1 Adult InseminatedFemale Spermatheca    2.1 Adult Ovary    1.5 Adult Testis    1.9 Adult Male Accessory Gland    1.7 Adult Carcass    2.1 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    3.2 Larval Midgut    1 Larval Hindgut    0.8 Larval Malpighian Tubules    2.2 Larval Fat Body    3.6 Larval Salivary Gland    9537.6 Larval Trachea    11.225 Larval Carcass    4.9 Adult Head    3.4 Adult Eye    2.425 Adult Brain    2.4 Adult Thoracic-Abdominal Ganglion    2.8 Adult Crop    3 Adult Midgut    3.1 Adult Hindgut    3.3 Adult Malpighian Tubules    2.5 Adult Fat Body    5.9 Adult Salivary Gland    2.7 Adult Heart    0.5 Adult VirginFemale Spermatheca    4.1 Adult InseminatedFemale Spermatheca    2.1 Adult Ovary    1.5 Adult Testis    1.9 Adult Male Accessory Gland    1.7 Adult Carcass    2.1 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    3.4   NA  Eye    2.425   NA  Brain    2.4   3.2  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    2.8   NA  Crop    3   1  Midgut    3.1   0.8  Hindgut    3.3   2.2  Malpighian Tubules    2.5   3.6  Fat Body    5.9   9537.6  Salivary Gland    2.7   NA  Heart    0.5   no informative data  Trachea    NA   NA  VirginFemale Spermatheca    4.1   NA  InseminatedFemale Spermatheca    2.1   NA  Ovary    1.5   NA  Testis    1.9   NA  Male Accessory Gland    1.7   4.9  Carcass    2.1 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    5 central nervous system, larvae L3    4 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    268 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    0 carcass, larvae L3 wandering    41 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    0 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    5 central nervous system, larvae L3    4 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering  (268) salivary gland, white prepupae    2 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    0 carcass, larvae L3 wandering  (41) carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    0 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    5 central nervous system, larvae L3    4 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering  (268) salivary gland, white prepupae    2 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    0 carcass, larvae L3 wandering    41 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    0 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    5 central nervous system, larvae L3    4 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    268 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    0 carcass, larvae L3 wandering    41 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    0 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    0 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    5 central nervous system, larvae L3    4 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    268 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    0 carcass, larvae L3 wandering    41 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    0 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    5 central nervous system, larvae L3    4 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering  (268) salivary gland, white prepupae    2 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    0 carcass, larvae L3 wandering  (41) carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    0 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    5 central nervous system, larvae L3    4 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering  (268) salivary gland, white prepupae    2 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    0 carcass, larvae L3 wandering    41 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    0 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    5 central nervous system, larvae L3    4 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    268 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    0 carcass, larvae L3 wandering    41 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    0 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of no expression detected. Peak expression observed during late larval stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    20 larva L3 puffstage 1-2    54 larva L3 puffstage 3-6    9 larva L3 puffstage 7-9    1 white prepupae new    0 white prepupae 12hr    0 white prepupae 24hr    0 pupae 2d postWPP    0 pupae 3d postWPP    0 pupae 4d postWPP    0 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    20 larva L3 puffstage 1-2  (54) larva L3 puffstage 3-6    9 larva L3 puffstage 7-9    1 white prepupae new    0 white prepupae 12hr    0 white prepupae 24hr    0 pupae 2d postWPP    0 pupae 3d postWPP    0 pupae 4d postWPP    0 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    20 larva L3 puffstage 1-2    54 larva L3 puffstage 3-6    9 larva L3 puffstage 7-9    1 white prepupae new    0 white prepupae 12hr    0 white prepupae 24hr    0 pupae 2d postWPP    0 pupae 3d postWPP    0 pupae 4d postWPP    0 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    20 larva L3 puffstage 1-2    54 larva L3 puffstage 3-6    9 larva L3 puffstage 7-9    1 white prepupae new    0 white prepupae 12hr    0 white prepupae 24hr    0 pupae 2d postWPP    0 pupae 3d postWPP    0 pupae 4d postWPP    0 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    20 larva L3 puffstage 1-2    54 larva L3 puffstage 3-6    9 larva L3 puffstage 7-9    1 white prepupae new    0 white prepupae 12hr    0 white prepupae 24hr    0 pupae 2d postWPP    0 pupae 3d postWPP    0 pupae 4d postWPP    0 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    20 larva L3 puffstage 1-2  (54) larva L3 puffstage 3-6    9 larva L3 puffstage 7-9    1 white prepupae new    0 white prepupae 12hr    0 white prepupae 24hr    0 pupae 2d postWPP    0 pupae 3d postWPP    0 pupae 4d postWPP    0 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    20 larva L3 puffstage 1-2    54 larva L3 puffstage 3-6    9 larva L3 puffstage 7-9    1 white prepupae new    0 white prepupae 12hr    0 white prepupae 24hr    0 pupae 2d postWPP    0 pupae 3d postWPP    0 pupae 4d postWPP    0 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    20 larva L3 puffstage 1-2    54 larva L3 puffstage 3-6    9 larva L3 puffstage 7-9    1 white prepupae new    0 white prepupae 12hr    0 white prepupae 24hr    0 pupae 2d postWPP    0 pupae 3d postWPP    0 pupae 4d postWPP    0 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    3 heat shock, 4-day adult    175 Cadmium 50 mM 6 hrs, larvae L3    186 Cadmium 50 mM 12 hrs, larvae L3    117 Cadmium 50 mM 48 hrs, 4-day adult    213 Cadmium 100 mM 48 hrs, 4-day adult    0 Copper 0.5 mM 12 hrs, larvae L3    282 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    348 Zinc 4.5 mM 48 hrs, 4-day adult    12 Ethanol 2.5% 3 hrs, larvae L3    0 Ethanol 5% 3 hrs, larvae L3    494 Ethanol 10% 3 hrs, larvae L3    384 Caffeine 1.5 mg/ml 4 hrs, larvae L3    420 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    0 Caffeine 25 mg/ml 48 hrs, 4-day adult    10 Paraquat 5 mM 48 hrs, 4-day adult    0 Paraquat 10 mM 48 hrs, 4-day adult    0 Rotenone 2 μg 12 hrs, larvae L3    204 Rotenone 8 μg 12 hrs, larvae L3    333 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    3 heat shock, 4-day adult  (175) Cadmium 50 mM 6 hrs, larvae L3  (186) Cadmium 50 mM 12 hrs, larvae L3  (117) Cadmium 50 mM 48 hrs, 4-day adult  (213) Cadmium 100 mM 48 hrs, 4-day adult    0 Copper 0.5 mM 12 hrs, larvae L3  (282) Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3  (348) Zinc 4.5 mM 48 hrs, 4-day adult    12 Ethanol 2.5% 3 hrs, larvae L3    0 Ethanol 5% 3 hrs, larvae L3  (494) Ethanol 10% 3 hrs, larvae L3  (384) Caffeine 1.5 mg/ml 4 hrs, larvae L3  (420) Caffeine 2.5 mg/ml 48 hrs, 4-day adult    0 Caffeine 25 mg/ml 48 hrs, 4-day adult    10 Paraquat 5 mM 48 hrs, 4-day adult    0 Paraquat 10 mM 48 hrs, 4-day adult    0 Rotenone 2 μg 12 hrs, larvae L3  (204) Rotenone 8 μg 12 hrs, larvae L3  (333) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    3 heat shock, 4-day adult  (175) Cadmium 50 mM 6 hrs, larvae L3  (186) Cadmium 50 mM 12 hrs, larvae L3  (117) Cadmium 50 mM 48 hrs, 4-day adult  (213) Cadmium 100 mM 48 hrs, 4-day adult    0 Copper 0.5 mM 12 hrs, larvae L3  (282) Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3  (348) Zinc 4.5 mM 48 hrs, 4-day adult    12 Ethanol 2.5% 3 hrs, larvae L3    0 Ethanol 5% 3 hrs, larvae L3  (494) Ethanol 10% 3 hrs, larvae L3  (384) Caffeine 1.5 mg/ml 4 hrs, larvae L3  (420) Caffeine 2.5 mg/ml 48 hrs, 4-day adult    0 Caffeine 25 mg/ml 48 hrs, 4-day adult    10 Paraquat 5 mM 48 hrs, 4-day adult    0 Paraquat 10 mM 48 hrs, 4-day adult    0 Rotenone 2 μg 12 hrs, larvae L3  (204) Rotenone 8 μg 12 hrs, larvae L3  (333) Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    3 heat shock, 4-day adult    175 Cadmium 50 mM 6 hrs, larvae L3    186 Cadmium 50 mM 12 hrs, larvae L3    117 Cadmium 50 mM 48 hrs, 4-day adult    213 Cadmium 100 mM 48 hrs, 4-day adult    0 Copper 0.5 mM 12 hrs, larvae L3    282 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    348 Zinc 4.5 mM 48 hrs, 4-day adult    12 Ethanol 2.5% 3 hrs, larvae L3    0 Ethanol 5% 3 hrs, larvae L3    494 Ethanol 10% 3 hrs, larvae L3    384 Caffeine 1.5 mg/ml 4 hrs, larvae L3    420 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    0 Caffeine 25 mg/ml 48 hrs, 4-day adult    10 Paraquat 5 mM 48 hrs, 4-day adult    0 Paraquat 10 mM 48 hrs, 4-day adult    0 Rotenone 2 μg 12 hrs, larvae L3    204 Rotenone 8 μg 12 hrs, larvae L3    333 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    3 heat shock, 4-day adult    175 Cadmium 50 mM 6 hrs, larvae L3    186 Cadmium 50 mM 12 hrs, larvae L3    117 Cadmium 50 mM 48 hrs, 4-day adult    213 Cadmium 100 mM 48 hrs, 4-day adult    0 Copper 0.5 mM 12 hrs, larvae L3    282 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    348 Zinc 4.5 mM 48 hrs, 4-day adult    12 Ethanol 2.5% 3 hrs, larvae L3    0 Ethanol 5% 3 hrs, larvae L3    494 Ethanol 10% 3 hrs, larvae L3    384 Caffeine 1.5 mg/ml 4 hrs, larvae L3    420 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    0 Caffeine 25 mg/ml 48 hrs, 4-day adult    10 Paraquat 5 mM 48 hrs, 4-day adult    0 Paraquat 10 mM 48 hrs, 4-day adult    0 Rotenone 2 μg 12 hrs, larvae L3    204 Rotenone 8 μg 12 hrs, larvae L3    333 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    3 heat shock, 4-day adult  (175) Cadmium 50 mM 6 hrs, larvae L3  (186) Cadmium 50 mM 12 hrs, larvae L3  (117) Cadmium 50 mM 48 hrs, 4-day adult  (213) Cadmium 100 mM 48 hrs, 4-day adult    0 Copper 0.5 mM 12 hrs, larvae L3  (282) Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3  (348) Zinc 4.5 mM 48 hrs, 4-day adult    12 Ethanol 2.5% 3 hrs, larvae L3    0 Ethanol 5% 3 hrs, larvae L3  (494) Ethanol 10% 3 hrs, larvae L3  (384) Caffeine 1.5 mg/ml 4 hrs, larvae L3  (420) Caffeine 2.5 mg/ml 48 hrs, 4-day adult    0 Caffeine 25 mg/ml 48 hrs, 4-day adult    10 Paraquat 5 mM 48 hrs, 4-day adult    0 Paraquat 10 mM 48 hrs, 4-day adult    0 Rotenone 2 μg 12 hrs, larvae L3  (204) Rotenone 8 μg 12 hrs, larvae L3  (333) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    3 heat shock, 4-day adult  (175) Cadmium 50 mM 6 hrs, larvae L3  (186) Cadmium 50 mM 12 hrs, larvae L3  117 Cadmium 50 mM 48 hrs, 4-day adult  (213) Cadmium 100 mM 48 hrs, 4-day adult    0 Copper 0.5 mM 12 hrs, larvae L3  (282) Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3  (348) Zinc 4.5 mM 48 hrs, 4-day adult    12 Ethanol 2.5% 3 hrs, larvae L3    0 Ethanol 5% 3 hrs, larvae L3  (494) Ethanol 10% 3 hrs, larvae L3  (384) Caffeine 1.5 mg/ml 4 hrs, larvae L3  (420) Caffeine 2.5 mg/ml 48 hrs, 4-day adult    0 Caffeine 25 mg/ml 48 hrs, 4-day adult    10 Paraquat 5 mM 48 hrs, 4-day adult    0 Paraquat 10 mM 48 hrs, 4-day adult    0 Rotenone 2 μg 12 hrs, larvae L3  (204) Rotenone 8 μg 12 hrs, larvae L3  (333) Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    3 heat shock, 4-day adult    175 Cadmium 50 mM 6 hrs, larvae L3    186 Cadmium 50 mM 12 hrs, larvae L3    117 Cadmium 50 mM 48 hrs, 4-day adult    213 Cadmium 100 mM 48 hrs, 4-day adult    0 Copper 0.5 mM 12 hrs, larvae L3    282 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    348 Zinc 4.5 mM 48 hrs, 4-day adult    12 Ethanol 2.5% 3 hrs, larvae L3    0 Ethanol 5% 3 hrs, larvae L3    494 Ethanol 10% 3 hrs, larvae L3    384 Caffeine 1.5 mg/ml 4 hrs, larvae L3    420 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    0 Caffeine 25 mg/ml 48 hrs, 4-day adult    10 Paraquat 5 mM 48 hrs, 4-day adult    0 Paraquat 10 mM 48 hrs, 4-day adult    0 Rotenone 2 μg 12 hrs, larvae L3    204 Rotenone 8 μg 12 hrs, larvae L3    333 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (Graveley et al., 2011) mE1_20_mRNA_expression_cluster_08
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0002933 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG10781-RA;CG10781-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Phenotype manifest in Allele
Classical Alleles ( 0 )
For All Classical Alleles Show
Allele of ng1 Class Mutagen Stocks Known lesion
Alleles Carried on Transgenic Constructs ( 6 )
For All Alleles Carried on Transgenic Constructs Show
Allele of ng1 Class Mutagen Stocks Known lesion ng1fusA.T:Ecol\lacZ in vitro construct - coding region fusion 0 Yes ng1KK115882 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes ng1mutA.T:Ecol\lacZ in vitro construct - site directed mutagenesis 0 Yes ng1pCa-Δα1.T:Ecol\lacZ in vitro construct - coding region fusion 0 Yes ng1pCaα.T:Ecol\lacZ in vitro construct - coding region fusion 0 Yes ng1Δ-fusA.T:Ecol\lacZ in vitro construct - coding region fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(1)N23-24c (Furia et al., 1993)
Not disrupted in	Df(1)N-54l9 (Furia et al., 1993)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD6398} NA P{GD13314} NA P{GD17311} NA P{GD17363} NA P{KK115882} NA reporter construct P{HZ50-ng-EcRE} No P{ng1-lacZ.} No P{ng1-lacZ.fusA} No P{ng1-lacZ.pCa-Δα1} No P{ng1-lacZ.pCaα} No P{ng1-lacZ.Δ-fusA} No P{ng1-lacZ.Δα2} No
Insertions
	Type of insertions Name Expression data
Gene Ontology: Function, Process & Cellular Component ( 3 unique terms )
Terms Based on Experimental Evidence ( 0 terms )
Terms Based on Predictions or Assertions ( 3 terms )
Molecular Function
CV term Evidence References
structural molecule activity non-traceable author statement (FlyBase, 1992-)
Biological Process
CV term Evidence References
puparial adhesion non-traceable author statement (FlyBase, 1992-)
Cellular Component
CV term Evidence References
extracellular region non-traceable author statement (Swiss-Prot Project Members, 1992.3.1)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions ng1 allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 57829 DroID - A comprehensive database of gene and protein interactions. • FBgn0002933 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 31300
Orthologs
OrthoDB Orthologs (0) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search No orthologs identified Dipteran inclusive ortholog search No orthologs identified Insect inclusive ortholog search No orthologs identified Arthropod inclusive ortholog search No orthologs identified Metazoa inclusive ortholog search No orthologs identified
Orthologs in Drosophila Species (None identified)
No orthologies identified
Orthologs in non-Drosophila Dipterans (None identified)
No non-Drosophilid orthologies identified
Orthologs in non-Dipteran Insects (None identified)
No non-Dipteran orthologies identified
Orthologs in non-Insect Arthropods (None identified)
No non-Insect Arthropod orthologies identified
Orthologs in non-Arthropod Metazoa (None identified)
No non-Arthropod Metazoa orthologies identified
Human Orthologs (0)
Gene OMIM HGNC
AAA Orthologs (0) based on analysis using Dmel annotation version 4.3
No orthologs identified
Stocks & Reagents
Stocks Listed in FlyBase ( 0 )
Genomic Clones ( 1 )
	BACR34B12 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 6 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	RE05239
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones
Other clones	IP01729 IP01730 IP01731 IP01732 RH11838
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0002933 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 31300
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments
Other Comments
	The binding properties of the ng-EcRE, a directly repeated half site (DR) located within the coding region of ng1, are studied to investigate the properties of EcREs composed of DRs. The ng-EcRE contacts the EcR/usp heterodimer through the DRs spaced by 12bp, this element may interact efficiently with Hr38 and Hr39. The ng-EcRE can promote functional interactions in vitro as well as in vivo, acting as a transcriptional enhancer able to confer a specific developmental expression profile to a minimal promoter in transgenic flies. (Crispi et al., 1998) ng1 RNA expression has been analysed in wild-type and br mutant larvae. (D'Avino et al., 1995) An ecdysone-responsive element is required for high level expression of the ng1 intermoult gene. (D'Avino et al., 1995) DNA-blotting assay has identified a high affinity ecdysone receptor binding site within the ng1 coding sequence. EMSA assay demonstrates the 93bp 'ng element' is able to bind an EcR/usp heterodimer and usp alone. (D'Avino et al., 1995) ng4 and ng1 originate by means of a gene duplication event. Also a strong similarity exists between ng1 and ng2 5' flanking regions. (Furia et al., 1993) An investigation of the relationship between transcription, puffing and hormone regulation of intermoult puff was analysed using ecd1 mutant embryos: ng1 expression is totally unaffected by shifting ecd1 embryos to the restrictive temperature, 30oC. (Furia et al., 1992)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_057307 RefSeq (Proteins) • NP_476655 DDBJ / EMBL / GenBank • AAF45856 AL024484 CAA19667 CAA36363 X52118 Entrez Gene - A searchable database of RefSeq genes. • 31300 UniProtKB/Swiss-Prot • P23938
Other Crossreferences
Linkouts
	BioGRID - A database of protein and genetic interactions • 57829 DroID - A comprehensive database of gene and protein interactions. • FBgn0002933 DRSC - Results from RNAi screens. • FBgn0002933 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0002933 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG10781-RA;CG10781-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0002933 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 31300 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 31300 modMine - Data generated by the modENCODE project. • FBgn0002933 REDfly - A database of transcriptional regulatory elements. • FBgn0002933
Synonyms & Secondary IDs ( 11 )
Reported As
Symbol Synonym	CG10781 (Keleman et al., 2009.8.5) EG:96G10.5   ng (Buszczak and Segraves, 1998, Wang et al., 1998) ng-1 (Kotlikova et al., 2006, Gibson et al., 2005, Xu et al., 2002, Crispi et al., 2001, Hall and Thummel, 1998, Fisk and Thummel, 1998, Furia et al., 1998, Crispi et al., 1998, D'Avino et al., 1995, D'Avino et al., 1995, Crispi et al., 1995, D'Avino et al., 1995, Nighorn et al., 1994, Furia et al., 1993, Furia et al., 1992, McBrayer et al., 2007, Yin et al., 2007) ng1 (Reiter et al., 2007, Kuan et al., 2009, Zhai et al., 2012, Jensen et al., 2007, Liu and Lehmann, 2008, Sharma et al., 2011) ngp (Furia et al., 1990, ) SC1 (Furia et al., 1990, )
Name Synonym	nested (Furia, 1990.3.14) new glue (Yin et al., 2007) new glue 1   new glue protein
Secondary FlyBase IDs
	FBgn0010372
References ( 42 )
Generate a list of	All references relating to this gene ( 42 )
List References by type	Research paper  ( 24 ) Supplementary material  ( 2 ) Review  ( 3 ) Personal communication to FlyBase  ( 2 ) Abstract  ( 4 ) FlyBase analysis  ( 2 ) DNA/RNA sequence record  ( 2 ) Protein sequence record  ( 1 ) Curated genome annotation  ( 2 )
Recent research papers ( 3 )
	Zhai et al., 2012, PLoS Genet. 8(3): e1002582 Antagonistic regulation of apoptosis and differentiation by the cut transcription factor represents a tumor-suppressing mechanism in Drosophila. [FBrf0217859] Hens et al., 2011, Nat. Methods 8(12): 1065--1070 Automated protein-DNA interaction screening of Drosophila regulatory elements. [FBrf0216759] Sharma et al., 2011, Chemosphere 82(3): 370--376 Transcriptome analysis provides insights for understanding the adverse effects of endosulfan in Drosophila melanogaster. [FBrf0212610] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...