• Home
• Tools
  • Tools Overview
  • Query Tools

    
    • QuickSearch
    • QueryBuilder
    • TermLink
    • CytoSearch
    • RNA-Seq Search
    • Interactions Browser
    • ImageBrowse
    • Find A Person
    • Google™ FlyBase
  • Genomic/Map Tools

    
    • BLAST
    • GBrowse
    • GBrowse 2 Beta
    • CytoSearch
    • Feature Mapper
    • Aberrations Maps
    • Chromosome Maps
    • Coordinates Converter
  • Retrieve/Convert

    
    • Batch Download
    • Coordinates Converter
    • ID Converter
  • Data Submission

    
    • Fast-Track Your Paper
    • Submit Personal Comm
    • Add A New Person
    • Update Person Info
  • People

    
    • Find A Person
    • Add A New Person
    • Update Person Info
• Files
  • Files Overview
  • Current release
  • Archived releases
  • Map Conversion
  • FTP site:
  • Releases (FTP)
  • Genomes (FTP)
• Species
  • Phylogeny
  • Sequenced Species
  • Synteny Table
  • Color Illustrations
  • Abbreviations
• Documents
  • About FlyBase
  • Reference Manual:
  • Sections
  • Contents
  • Nomenclature
  • Release Notes
• Resources
  • All Resources
  • Model Organisms
  • Stock Collections
  • Vectors & Constructs
  • Other links:
  • BDGP
  • DGRC
  • DIS by issue
  • FlyExpress
  • Interactive Fly
  • modENCODE
  • Textpresso for Fly
• News
  • News
  • FlyBase Forum
  • FlyBase Positions
  • Meetings
  • Courses
  • Fly Board
  • White papers
  • Funding
  • bionet.dros
• Help
  • Google™ FlyBase
  • Site Map
  • FAQs
  • FlyBase Guides
  • Video Tutorials
  • Report help
  • New to Flies
  • Pers. comm.
  • Author guidelines
  • Contact FlyBase
• Archives
  • Prior Release
  • Other Archives
  • Coordinate Converter

Gene Dmel\nod

General Information
Symbol	Dmel\nod	Species	D. melanogaster
Name	no distributive disjunction	Annotation symbol	CG1763
Feature type	protein_coding_gene	FlyBase ID	FBgn0002948
Gene Model Status	Current	Stock availability	17 publicly available
Also Known As	DmNod, l(1)TW6
Genomic Location
Chromosome (arm)	X	Recombination map	1-36
Cytogenetic map	10C7-10C8	Sequence location	X:11,474,805..11,479,783 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene no distributive disjunction is referred to in FlyBase by the symbol Dmel\nod (CG1763, FBgn0002948). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: microtubule plus-end binding; microtubule motor activity. There is experimental evidence that it is involved in the biological process: meiotic chromosome segregation; spindle assembly involved in female meiosis I; distributive segregation; establishment of meiotic spindle orientation; mitotic spindle organization; positive regulation of microtubule polymerization. 38 alleles are reported. The phenotype of these alleles is annotated with: polytene chromosome chromocenter. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: Helix-hairpin-helix DNA-binding motif, class 1; Kinesin, motor domain; Kinesin, motor region, conserved site. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of very low expression. Peak expression observed within 00-06 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult ovary. Comments on Affy2 ProbeSet: ProbeSet 1639689_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of nod. Gene sequence location is X:11474805..11479783.
User Contributed Data
	Beta version, feedback welcome
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	nod: no distributive disjunction (R.S. Hawley) Females homozygous for nod alleles exhibit high frequencies of meiotic chromosome loss and nondisjunction at meiosis I. Most nod-induced nondisjunctional events involve nonexchange chromosomes. For example, in nod/nod females nondisjunction frequencies for the always nonexchange fourth chromosomes approaches 90% (the vast majority of gametes are nullo-4 ova), whereas nonexchange X chromosomes apparently disjoin at random. Both the frequency of exchange and the disjunction of exchange bivalents was shown to be normal in nod/nod females. Thus, with respect to its role in meiosis, the nod+ function appears to be limited to the distributive segregation system. Based on an analysis of secondary nondisjunction in noda/noda females, Carpenter concluded that the nod defect does not impair the process of partner choice within the distributive system, but rather specifically impairs the disjunctional process. Nonexchange chromosomes derived from noda/noda mothers also undergo nondisjunction, and presumably loss, at meiosis II. In addition, chromosomes derived from noda/noda mothers are mitotically unstable. nod-induced mitotic chromosome loss is restricted to maternal nonexchange chromosomes and does not exert any discernable effect on meiosis in males or on mitotic chromosome stability (Baker et al., 1978). Although none of the existing nod alleles is lethal or female sterile, the dosage-sensitive antimorphic mutation l(1)TW6 (Wright, 1973) is argued to be allelic to nod on the basis of three lines of evidence. First, l(1)TW6/+ females display a meiotic phenotype that is virtually identical to that exhibited by noda/noda females. Second, the two loci map to the same position on the X chromosome (Wright, 1973; Baker). Third, a γ-ray induced revertant of l(1)TW6 was shown to be a recessive nod allele (New and Hawley).
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 10C7-10C8
Experimentally Determined Cytological Location
Cytogenetic map Notes References 10A-10A   (determined by in situ hybridisation)   (Stewart et al., 1991) 10C5-10C8   (determined by in situ hybridisation)   (Endow and Hatsumi, 1991) 10C2-10C5   (determined by in situ hybridisation)   (Zhang et al., 1990)
Experimentally Determined Recombination Data
Location	1-35.5 (Rasooly et al., 1991) 1-36
Left of (cM)	m (Rasooly et al., 1991)
Right of (cM)	v (Rasooly et al., 1991)
Notes	Based on mapping of nodDTW. (Rasooly et al., 1991)
Gene Model & Products
Please see the GBrowse view of Dmel\nod for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.50 Low-frequency RNA-Seq exon junction(s) not annotated.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) nod-RA FBtr0073516  NM_078570   2449   666 nod-RB FBtr0340297     2279   666
Additional Transcript Data & Comments
Reported size (kB)	2.4 (northern blot) (Zhang et al., 1990)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein nod-PA   FBpp0073363   73.9   666   10.19     NP_511125   AAF48064 nod-PB   FBpp0309258   73.9   666   10.19
Additional Polypeptide Data & Comments
Reported size (kDa)	666 (aa); 74 (kD) (Zhang et al., 1990)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Kinesin, motor domain (IPR001752) Helix-hairpin-helix DNA-binding motif, class 1 (IPR003583) Kinesin, motor region, conserved site (IPR019821) P-loop containing nucleoside triphosphate hydrolase (IPR027417)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA141291   AA201882   AA801994   AA950920   AE014298 AAF48064   AI531306   AW944230   AY050238 AAK84937   BG640531   BI167517   BI213910   BI367745   BI568461   BI570170   BT056296   CL706558   CO271290   CO274273   CO279739   CO283365   CO304817   CO307371   CO320535   CO323383   CO324149   CO325655   CZ481108   CZ481109   CZ483901   DN154491   EC057804   EC061999   EC063230   EC071949   EC074606   EC077176   EC077822   EC078853   EC083696   EC089010   EC090088   EC232088   EC246907   EL883291   EV575363   GH749196   GH750370   GH750851   GH777438   GH788696   GH791316   GH794980   GH800021   GH800037   GH808074   GH817445   GH822129   GH829190   GH835341   GH846114   GH846692   GH848561   GH850903   GH851674   GH856355   GH859829   GH866691   GH868011   GH872293   GH878924   GH881277   GH882069   GH887632   GH898633   GH903157   GH904783   GH916350   GH916477   GH920311   GH923419   GH923652   GH927788   GH929576   GH930025   GH930101   GH930838   M36195 AAA28653   M94188 AAC14452
UniProtKB/Swiss-Prot	P18105
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
No Assay Recorded Stage Tissue/Position (including subcellular localization) Reference embryonic stage -- adult stage     (Zhang et al., 1990) adult stage & oogenesis   germarium   (Zhang et al., 1990) adult stageoogenesis stage S1 -- S3   ovariole   (Zhang et al., 1990) in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 4 -- 6   central brain anlage in statu nascendi   (Fisher et al., 2012) dorsal ectoderm anlage   (Fisher et al., 2012) Comment:anlage in statu nascendi ventral ectoderm anlage   (Fisher et al., 2012) Comment:anlage in statu nascendi antennal anlage in statu nascendi   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage in statu nascendi dorsal head epidermis anlage in statu nascendi   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage in statu nascendi visual anlage in statu nascendi   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage in statu nascendi embryonic stage 7 -- 8   central brain anlage   (Fisher et al., 2012) head mesoderm   (Fisher et al., 2012) organism | faint & ubiquitous   (Fisher et al., 2012) trunk mesoderm   (Fisher et al., 2012) ventral ectoderm   (Fisher et al., 2012) embryonic stage 9 -- 10   central brain primordium   (Fisher et al., 2012) head mesoderm   (Fisher et al., 2012) organism | faint & ubiquitous   (Fisher et al., 2012) trunk mesoderm   (Fisher et al., 2012) ventral nerve cord primordium   (Fisher et al., 2012) embryonic stage 11 -- 12   central brain primordium   (Fisher et al., 2012) ventral nerve cord primordium   (Fisher et al., 2012) embryonic stage 13 -- 16   embryonic brain   (Fisher et al., 2012) embryonic/larval nervous system   (Fisher et al., 2012) ventral nerve cord   (Fisher et al., 2012)
Additional Descriptive Data
	The 2.4kb nod transcript is present in In(1)nod-bd/Df(1)nod females. (Zhang et al., 1990) nod transcripts are detected in the germarium and in oogenesis stages S1-S3. Expression is again observed in stage S9-S12 egg chambers primarily in the nurse cell cytoplasm. By stage 12, the nurse cells have degenerated and nod transcripts are detected in the ooplasm. nod transcripts are detected throughout development with significantly higher levels in embryos and adult females than in other stages. (Zhang et al., 1990) The 2.4kb transcript is increased in size and present at reduced levels in nod3/Df(1)nod females. (Zhang et al., 1990) The 2.4kb nod transcript is greatly reduced or absent in nod2/FBab0000863:Df(1)nod females. Only very faint staining is observed in ovaries from FBal0013066:nod2@ homozygous females. (Zhang et al., 1990)
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult ovary. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    79.275 Larval Midgut    10 Larval Hindgut    20.7 Larval Malpighian Tubules    10.1 Larval Fat Body    16.1 Larval Salivary Gland    11.8 Larval Trachea    27 Larval Carcass    19.125 Adult Head    16.7 Adult Eye    33.275 Adult Brain    15.2 Adult Thoracic-Abdominal Ganglion    22.7 Adult Crop    37.3 Adult Midgut    11.9 Adult Hindgut    20.2 Adult Malpighian Tubules    11.8 Adult Fat Body    25.9 Adult Salivary Gland    20.4 Adult Heart    16.475 Adult VirginFemale Spermatheca    18.1 Adult InseminatedFemale Spermatheca    14.4 Adult Ovary    453.7 Adult Testis    10.9 Adult Male Accessory Gland    20.7 Adult Carcass    35.3 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    79.275 Larval Midgut    10 Larval Hindgut    20.7 Larval Malpighian Tubules    10.1 Larval Fat Body    16.1 Larval Salivary Gland    11.8 Larval Trachea    27 Larval Carcass    19.125 Adult Head    16.7 Adult Eye    33.275 Adult Brain    15.2 Adult Thoracic-Abdominal Ganglion    22.7 Adult Crop    37.3 Adult Midgut    11.9 Adult Hindgut    20.2 Adult Malpighian Tubules    11.8 Adult Fat Body    25.9 Adult Salivary Gland    20.4 Adult Heart    16.475 Adult VirginFemale Spermatheca    18.1 Adult InseminatedFemale Spermatheca    14.4 Adult Ovary    453.7 Adult Testis    10.9 Adult Male Accessory Gland    20.7 Adult Carcass    35.3 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    79.275 Larval Midgut    10 Larval Hindgut    20.7 Larval Malpighian Tubules    10.1 Larval Fat Body    16.1 Larval Salivary Gland    11.8 Larval Trachea    27 Larval Carcass    19.125 Adult Head    16.7 Adult Eye    33.275 Adult Brain    15.2 Adult Thoracic-Abdominal Ganglion    22.7 Adult Crop    37.3 Adult Midgut    11.9 Adult Hindgut    20.2 Adult Malpighian Tubules    11.8 Adult Fat Body    25.9 Adult Salivary Gland    20.4 Adult Heart    16.475 Adult VirginFemale Spermatheca    18.1 Adult InseminatedFemale Spermatheca    14.4 Adult Ovary    453.7 Adult Testis    10.9 Adult Male Accessory Gland    20.7 Adult Carcass    35.3 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    79.275 Larval Midgut    10 Larval Hindgut    20.7 Larval Malpighian Tubules    10.1 Larval Fat Body    16.1 Larval Salivary Gland    11.8 Larval Trachea    27 Larval Carcass    19.125 Adult Head    16.7 Adult Eye    33.275 Adult Brain    15.2 Adult Thoracic-Abdominal Ganglion    22.7 Adult Crop    37.3 Adult Midgut    11.9 Adult Hindgut    20.2 Adult Malpighian Tubules    11.8 Adult Fat Body    25.9 Adult Salivary Gland    20.4 Adult Heart    16.475 Adult VirginFemale Spermatheca    18.1 Adult InseminatedFemale Spermatheca    14.4 Adult Ovary    453.7 Adult Testis    10.9 Adult Male Accessory Gland    20.7 Adult Carcass    35.3 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    79.275 Larval Midgut    10 Larval Hindgut    20.7 Larval Malpighian Tubules    10.1 Larval Fat Body    16.1 Larval Salivary Gland    11.8 Larval Trachea    27 Larval Carcass    19.125 Adult Head    16.7 Adult Eye    33.275 Adult Brain    15.2 Adult Thoracic-Abdominal Ganglion    22.7 Adult Crop    37.3 Adult Midgut    11.9 Adult Hindgut    20.2 Adult Malpighian Tubules    11.8 Adult Fat Body    25.9 Adult Salivary Gland    20.4 Adult Heart    16.475 Adult VirginFemale Spermatheca    18.1 Adult InseminatedFemale Spermatheca    14.4 Adult Ovary    453.7 Adult Testis    10.9 Adult Male Accessory Gland    20.7 Adult Carcass    35.3 Expression Level Scale  None   Low   Moderate   High  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    79.275 Larval Midgut    10 Larval Hindgut    20.7 Larval Malpighian Tubules    10.1 Larval Fat Body    16.1 Larval Salivary Gland    11.8 Larval Trachea    27 Larval Carcass    19.125 Adult Head    16.7 Adult Eye    33.275 Adult Brain    15.2 Adult Thoracic-Abdominal Ganglion    22.7 Adult Crop    37.3 Adult Midgut    11.9 Adult Hindgut    20.2 Adult Malpighian Tubules    11.8 Adult Fat Body    25.9 Adult Salivary Gland    20.4 Adult Heart    16.475 Adult VirginFemale Spermatheca    18.1 Adult InseminatedFemale Spermatheca    14.4 Adult Ovary    453.7 Adult Testis    10.9 Adult Male Accessory Gland    20.7 Adult Carcass    35.3 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    79.275 Larval Midgut    10 Larval Hindgut    20.7 Larval Malpighian Tubules    10.1 Larval Fat Body    16.1 Larval Salivary Gland    11.8 Larval Trachea    27 Larval Carcass    19.125 Adult Head    16.7 Adult Eye    33.275 Adult Brain    15.2 Adult Thoracic-Abdominal Ganglion    22.7 Adult Crop    37.3 Adult Midgut    11.9 Adult Hindgut    20.2 Adult Malpighian Tubules    11.8 Adult Fat Body    25.9 Adult Salivary Gland    20.4 Adult Heart    16.475 Adult VirginFemale Spermatheca    18.1 Adult InseminatedFemale Spermatheca    14.4 Adult Ovary    453.7 Adult Testis    10.9 Adult Male Accessory Gland    20.7 Adult Carcass    35.3 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    79.275 Larval Midgut    10 Larval Hindgut    20.7 Larval Malpighian Tubules    10.1 Larval Fat Body    16.1 Larval Salivary Gland    11.8 Larval Trachea    27 Larval Carcass    19.125 Adult Head    16.7 Adult Eye    33.275 Adult Brain    15.2 Adult Thoracic-Abdominal Ganglion    22.7 Adult Crop    37.3 Adult Midgut    11.9 Adult Hindgut    20.2 Adult Malpighian Tubules    11.8 Adult Fat Body    25.9 Adult Salivary Gland    20.4 Adult Heart    16.475 Adult VirginFemale Spermatheca    18.1 Adult InseminatedFemale Spermatheca    14.4 Adult Ovary    453.7 Adult Testis    10.9 Adult Male Accessory Gland    20.7 Adult Carcass    35.3 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    16.7   NA  Eye    33.275   NA  Brain    15.2   79.275  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    22.7   NA  Crop    37.3   no informative data  Midgut    no informative data   20.7  Hindgut    20.2   no informative data  Malpighian Tubules    no informative data   no informative data  Fat Body    25.9   11.8  Salivary Gland    no informative data   NA  Heart    16.475   27  Trachea    NA   NA  VirginFemale Spermatheca    18.1   NA  InseminatedFemale Spermatheca    no informative data   NA  Ovary    453.7   NA  Testis    10.9   NA  Male Accessory Gland    20.7   19.125  Carcass    35.3 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    4 central nervous system, larvae L3    3 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    1 head, mated 4-day male    1 head, mated 20-day male    1 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    1 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    1 fat body, pupae P8    0 carcass, larvae L3 wandering    3 carcass, 1-day adult    1 carcass, 4-day adult    2 carcass, 20-day adult    1 ovary, virgin 4-day female    37 ovary, mated 4-day female    31 testis, mated 4-day male    0 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    4 central nervous system, larvae L3    3 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    1 head, mated 4-day male    1 head, mated 20-day male    1 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    1 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    1 fat body, pupae P8    0 carcass, larvae L3 wandering    3 carcass, 1-day adult    1 carcass, 4-day adult    2 carcass, 20-day adult    1 ovary, virgin 4-day female  (37) ovary, mated 4-day female  (31) testis, mated 4-day male    0 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    4 central nervous system, larvae L3    3 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    1 head, mated 4-day male    1 head, mated 20-day male    1 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    1 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    1 fat body, pupae P8    0 carcass, larvae L3 wandering    3 carcass, 1-day adult    1 carcass, 4-day adult    2 carcass, 20-day adult    1 ovary, virgin 4-day female    37 ovary, mated 4-day female    31 testis, mated 4-day male    0 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    4 central nervous system, larvae L3    3 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    1 head, mated 4-day male    1 head, mated 20-day male    1 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    1 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    1 fat body, pupae P8    0 carcass, larvae L3 wandering    3 carcass, 1-day adult    1 carcass, 4-day adult    2 carcass, 20-day adult    1 ovary, virgin 4-day female    37 ovary, mated 4-day female    31 testis, mated 4-day male    0 accessory gland, mated 4-day male    0 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    4 central nervous system, larvae L3    3 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    1 head, mated 4-day male    1 head, mated 20-day male    1 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    1 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    1 fat body, pupae P8    0 carcass, larvae L3 wandering    3 carcass, 1-day adult    1 carcass, 4-day adult    2 carcass, 20-day adult    1 ovary, virgin 4-day female    37 ovary, mated 4-day female    31 testis, mated 4-day male    0 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    4 central nervous system, larvae L3    3 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    1 head, mated 4-day male    1 head, mated 20-day male    1 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    1 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    1 fat body, pupae P8    0 carcass, larvae L3 wandering    3 carcass, 1-day adult    1 carcass, 4-day adult    2 carcass, 20-day adult    1 ovary, virgin 4-day female  (37) ovary, mated 4-day female  31 testis, mated 4-day male    0 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    4 central nervous system, larvae L3    3 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    1 head, mated 4-day male    1 head, mated 20-day male    1 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    1 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    1 fat body, pupae P8    0 carcass, larvae L3 wandering    3 carcass, 1-day adult    1 carcass, 4-day adult    2 carcass, 20-day adult    1 ovary, virgin 4-day female    37 ovary, mated 4-day female    31 testis, mated 4-day male    0 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    4 central nervous system, larvae L3    3 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    1 head, mated 4-day male    1 head, mated 20-day male    1 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    1 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    1 fat body, pupae P8    0 carcass, larvae L3 wandering    3 carcass, 1-day adult    1 carcass, 4-day adult    2 carcass, 20-day adult    1 ovary, virgin 4-day female    37 ovary, mated 4-day female    31 testis, mated 4-day male    0 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of very low expression. Peak expression observed within 00-06 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    101 embryo 02-04hr    8 embryo 04-06hr    24 embryo 06-08hr    29 embryo 08-10hr    18 embryo 10-12hr    9 embryo 12-14hr    4 embryo 14-16hr    2 embryo 16-18hr    1 embryo 18-20hr    1 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    3 larva L3 puffstage 7-9    3 white prepupae new    3 white prepupae 12hr    6 white prepupae 24hr    5 pupae 2d postWPP    1 pupae 3d postWPP    1 pupae 4d postWPP    2 adult male 01day    1 adult male 05day    2 adult male 30day    3 adult female 01day    20 adult female 05day    42 adult female 30day    44 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (101) embryo 02-04hr    8 embryo 04-06hr    24 embryo 06-08hr  (29) embryo 08-10hr    18 embryo 10-12hr    9 embryo 12-14hr    4 embryo 14-16hr    2 embryo 16-18hr    1 embryo 18-20hr    1 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    3 larva L3 puffstage 7-9    3 white prepupae new    3 white prepupae 12hr    6 white prepupae 24hr    5 pupae 2d postWPP    1 pupae 3d postWPP    1 pupae 4d postWPP    2 adult male 01day    1 adult male 05day    2 adult male 30day    3 adult female 01day    20 adult female 05day  (42) adult female 30day  (44) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr  101 embryo 02-04hr    8 embryo 04-06hr    24 embryo 06-08hr    29 embryo 08-10hr    18 embryo 10-12hr    9 embryo 12-14hr    4 embryo 14-16hr    2 embryo 16-18hr    1 embryo 18-20hr    1 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    3 larva L3 puffstage 7-9    3 white prepupae new    3 white prepupae 12hr    6 white prepupae 24hr    5 pupae 2d postWPP    1 pupae 3d postWPP    1 pupae 4d postWPP    2 adult male 01day    1 adult male 05day    2 adult male 30day    3 adult female 01day    20 adult female 05day    42 adult female 30day    44 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    101 embryo 02-04hr    8 embryo 04-06hr    24 embryo 06-08hr    29 embryo 08-10hr    18 embryo 10-12hr    9 embryo 12-14hr    4 embryo 14-16hr    2 embryo 16-18hr    1 embryo 18-20hr    1 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    3 larva L3 puffstage 7-9    3 white prepupae new    3 white prepupae 12hr    6 white prepupae 24hr    5 pupae 2d postWPP    1 pupae 3d postWPP    1 pupae 4d postWPP    2 adult male 01day    1 adult male 05day    2 adult male 30day    3 adult female 01day    20 adult female 05day    42 adult female 30day    44 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    101 embryo 02-04hr    8 embryo 04-06hr    24 embryo 06-08hr    29 embryo 08-10hr    18 embryo 10-12hr    9 embryo 12-14hr    4 embryo 14-16hr    2 embryo 16-18hr    1 embryo 18-20hr    1 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    3 larva L3 puffstage 7-9    3 white prepupae new    3 white prepupae 12hr    6 white prepupae 24hr    5 pupae 2d postWPP    1 pupae 3d postWPP    1 pupae 4d postWPP    2 adult male 01day    1 adult male 05day    2 adult male 30day    3 adult female 01day    20 adult female 05day    42 adult female 30day    44 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (101) embryo 02-04hr    8 embryo 04-06hr    24 embryo 06-08hr  29 embryo 08-10hr    18 embryo 10-12hr    9 embryo 12-14hr    4 embryo 14-16hr    2 embryo 16-18hr    1 embryo 18-20hr    1 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    3 larva L3 puffstage 7-9    3 white prepupae new    3 white prepupae 12hr    6 white prepupae 24hr    5 pupae 2d postWPP    1 pupae 3d postWPP    1 pupae 4d postWPP    2 adult male 01day    1 adult male 05day    2 adult male 30day    3 adult female 01day    20 adult female 05day  (42) adult female 30day  (44) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr  101 embryo 02-04hr    8 embryo 04-06hr    24 embryo 06-08hr    29 embryo 08-10hr    18 embryo 10-12hr    9 embryo 12-14hr    4 embryo 14-16hr    2 embryo 16-18hr    1 embryo 18-20hr    1 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    3 larva L3 puffstage 7-9    3 white prepupae new    3 white prepupae 12hr    6 white prepupae 24hr    5 pupae 2d postWPP    1 pupae 3d postWPP    1 pupae 4d postWPP    2 adult male 01day    1 adult male 05day    2 adult male 30day    3 adult female 01day    20 adult female 05day    42 adult female 30day    44 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    101 embryo 02-04hr    8 embryo 04-06hr    24 embryo 06-08hr    29 embryo 08-10hr    18 embryo 10-12hr    9 embryo 12-14hr    4 embryo 14-16hr    2 embryo 16-18hr    1 embryo 18-20hr    1 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    3 larva L3 puffstage 7-9    3 white prepupae new    3 white prepupae 12hr    6 white prepupae 24hr    5 pupae 2d postWPP    1 pupae 3d postWPP    1 pupae 4d postWPP    2 adult male 01day    1 adult male 05day    2 adult male 30day    3 adult female 01day    20 adult female 05day    42 adult female 30day    44 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    5 Schneider line 2 Sg4    7 embryonic 1182-4H    4 embryonic GM2    5 embryonic Kc167    2 embryonic S1    23 embryonic S3    4 leg disc CME L1    6 wing disc CME-W2    4 wing disc ML-DmD8    5 wing disc ML-DmD9    7 wing disc ML-DmD16-c3    5 wing disc ML-DmD21    6 wing disc ML-DmD32    4 haltere disc ML-DmD17-c3    3 eye-antennal disc ML-DmD11    5 antennal disc ML-DmD20-c5    4 mixed discs ML-DmD4-c1    4 CNS ML-DmBG1-c1    8 CNS ML-DmBG2-c2    2 tumorous blood cells mbn2    3 ovary fGS/OSS    7 ovary OSC    3 ovary OSS    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    5 Schneider line 2 Sg4    7 embryonic 1182-4H    4 embryonic GM2    5 embryonic Kc167    2 embryonic S1    23 embryonic S3    4 leg disc CME L1    6 wing disc CME-W2    4 wing disc ML-DmD8    5 wing disc ML-DmD9    7 wing disc ML-DmD16-c3    5 wing disc ML-DmD21    6 wing disc ML-DmD32    4 haltere disc ML-DmD17-c3    3 eye-antennal disc ML-DmD11    5 antennal disc ML-DmD20-c5    4 mixed discs ML-DmD4-c1    4 CNS ML-DmBG1-c1    8 CNS ML-DmBG2-c2    2 tumorous blood cells mbn2    3 ovary fGS/OSS    7 ovary OSC    3 ovary OSS    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    5 Schneider line 2 Sg4    7 embryonic 1182-4H    4 embryonic GM2    5 embryonic Kc167    2 embryonic S1    23 embryonic S3    4 leg disc CME L1    6 wing disc CME-W2    4 wing disc ML-DmD8    5 wing disc ML-DmD9    7 wing disc ML-DmD16-c3    5 wing disc ML-DmD21    6 wing disc ML-DmD32    4 haltere disc ML-DmD17-c3    3 eye-antennal disc ML-DmD11    5 antennal disc ML-DmD20-c5    4 mixed discs ML-DmD4-c1    4 CNS ML-DmBG1-c1    8 CNS ML-DmBG2-c2    2 tumorous blood cells mbn2    3 ovary fGS/OSS    7 ovary OSC    3 ovary OSS    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    5 Schneider line 2 Sg4    7 embryonic 1182-4H    4 embryonic GM2    5 embryonic Kc167    2 embryonic S1    23 embryonic S3    4 leg disc CME L1    6 wing disc CME-W2    4 wing disc ML-DmD8    5 wing disc ML-DmD9    7 wing disc ML-DmD16-c3    5 wing disc ML-DmD21    6 wing disc ML-DmD32    4 haltere disc ML-DmD17-c3    3 eye-antennal disc ML-DmD11    5 antennal disc ML-DmD20-c5    4 mixed discs ML-DmD4-c1    4 CNS ML-DmBG1-c1    8 CNS ML-DmBG2-c2    2 tumorous blood cells mbn2    3 ovary fGS/OSS    7 ovary OSC    3 ovary OSS    3 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    5 Schneider line 2 Sg4    7 embryonic 1182-4H    4 embryonic GM2    5 embryonic Kc167    2 embryonic S1    23 embryonic S3    4 leg disc CME L1    6 wing disc CME-W2    4 wing disc ML-DmD8    5 wing disc ML-DmD9    7 wing disc ML-DmD16-c3    5 wing disc ML-DmD21    6 wing disc ML-DmD32    4 haltere disc ML-DmD17-c3    3 eye-antennal disc ML-DmD11    5 antennal disc ML-DmD20-c5    4 mixed discs ML-DmD4-c1    4 CNS ML-DmBG1-c1    8 CNS ML-DmBG2-c2    2 tumorous blood cells mbn2    3 ovary fGS/OSS    7 ovary OSC    3 ovary OSS    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    5 Schneider line 2 Sg4    7 embryonic 1182-4H    4 embryonic GM2    5 embryonic Kc167    2 embryonic S1    23 embryonic S3    4 leg disc CME L1    6 wing disc CME-W2    4 wing disc ML-DmD8    5 wing disc ML-DmD9    7 wing disc ML-DmD16-c3    5 wing disc ML-DmD21    6 wing disc ML-DmD32    4 haltere disc ML-DmD17-c3    3 eye-antennal disc ML-DmD11    5 antennal disc ML-DmD20-c5    4 mixed discs ML-DmD4-c1    4 CNS ML-DmBG1-c1    8 CNS ML-DmBG2-c2    2 tumorous blood cells mbn2    3 ovary fGS/OSS    7 ovary OSC    3 ovary OSS    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    5 Schneider line 2 Sg4    7 embryonic 1182-4H    4 embryonic GM2    5 embryonic Kc167    2 embryonic S1    23 embryonic S3    4 leg disc CME L1    6 wing disc CME-W2    4 wing disc ML-DmD8    5 wing disc ML-DmD9    7 wing disc ML-DmD16-c3    5 wing disc ML-DmD21    6 wing disc ML-DmD32    4 haltere disc ML-DmD17-c3    3 eye-antennal disc ML-DmD11    5 antennal disc ML-DmD20-c5    4 mixed discs ML-DmD4-c1    4 CNS ML-DmBG1-c1    8 CNS ML-DmBG2-c2    2 tumorous blood cells mbn2    3 ovary fGS/OSS    7 ovary OSC    3 ovary OSS    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    5 Schneider line 2 Sg4    7 embryonic 1182-4H    4 embryonic GM2    5 embryonic Kc167    2 embryonic S1    23 embryonic S3    4 leg disc CME L1    6 wing disc CME-W2    4 wing disc ML-DmD8    5 wing disc ML-DmD9    7 wing disc ML-DmD16-c3    5 wing disc ML-DmD21    6 wing disc ML-DmD32    4 haltere disc ML-DmD17-c3    3 eye-antennal disc ML-DmD11    5 antennal disc ML-DmD20-c5    4 mixed discs ML-DmD4-c1    4 CNS ML-DmBG1-c1    8 CNS ML-DmBG2-c2    2 tumorous blood cells mbn2    3 ovary fGS/OSS    7 ovary OSC    3 ovary OSS    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    7 cold shock, 4-day adult    4 heat shock, 4-day adult    8 Cadmium 50 mM 6 hrs, larvae L3    0 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    10 Cadmium 100 mM 48 hrs, 4-day adult    25 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    7 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    2 Ethanol 2.5% 3 hrs, larvae L3    29 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    19 Caffeine 25 mg/ml 48 hrs, 4-day adult    7 Paraquat 5 mM 48 hrs, 4-day adult    18 Paraquat 10 mM 48 hrs, 4-day adult    15 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    7 cold shock, 4-day adult    4 heat shock, 4-day adult    8 Cadmium 50 mM 6 hrs, larvae L3    0 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    10 Cadmium 100 mM 48 hrs, 4-day adult    25 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    7 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    2 Ethanol 2.5% 3 hrs, larvae L3  (29) Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    19 Caffeine 25 mg/ml 48 hrs, 4-day adult    7 Paraquat 5 mM 48 hrs, 4-day adult    18 Paraquat 10 mM 48 hrs, 4-day adult    15 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    7 cold shock, 4-day adult    4 heat shock, 4-day adult    8 Cadmium 50 mM 6 hrs, larvae L3    0 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    10 Cadmium 100 mM 48 hrs, 4-day adult    25 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    7 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    2 Ethanol 2.5% 3 hrs, larvae L3    29 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    19 Caffeine 25 mg/ml 48 hrs, 4-day adult    7 Paraquat 5 mM 48 hrs, 4-day adult    18 Paraquat 10 mM 48 hrs, 4-day adult    15 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    7 cold shock, 4-day adult    4 heat shock, 4-day adult    8 Cadmium 50 mM 6 hrs, larvae L3    0 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    10 Cadmium 100 mM 48 hrs, 4-day adult    25 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    7 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    2 Ethanol 2.5% 3 hrs, larvae L3    29 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    19 Caffeine 25 mg/ml 48 hrs, 4-day adult    7 Paraquat 5 mM 48 hrs, 4-day adult    18 Paraquat 10 mM 48 hrs, 4-day adult    15 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    7 cold shock, 4-day adult    4 heat shock, 4-day adult    8 Cadmium 50 mM 6 hrs, larvae L3    0 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    10 Cadmium 100 mM 48 hrs, 4-day adult    25 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    7 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    2 Ethanol 2.5% 3 hrs, larvae L3    29 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    19 Caffeine 25 mg/ml 48 hrs, 4-day adult    7 Paraquat 5 mM 48 hrs, 4-day adult    18 Paraquat 10 mM 48 hrs, 4-day adult    15 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    7 cold shock, 4-day adult    4 heat shock, 4-day adult    8 Cadmium 50 mM 6 hrs, larvae L3    0 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    10 Cadmium 100 mM 48 hrs, 4-day adult    25 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    7 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    2 Ethanol 2.5% 3 hrs, larvae L3  29 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    19 Caffeine 25 mg/ml 48 hrs, 4-day adult    7 Paraquat 5 mM 48 hrs, 4-day adult    18 Paraquat 10 mM 48 hrs, 4-day adult    15 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    7 cold shock, 4-day adult    4 heat shock, 4-day adult    8 Cadmium 50 mM 6 hrs, larvae L3    0 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    10 Cadmium 100 mM 48 hrs, 4-day adult    25 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    7 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    2 Ethanol 2.5% 3 hrs, larvae L3    29 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    19 Caffeine 25 mg/ml 48 hrs, 4-day adult    7 Paraquat 5 mM 48 hrs, 4-day adult    18 Paraquat 10 mM 48 hrs, 4-day adult    15 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    7 cold shock, 4-day adult    4 heat shock, 4-day adult    8 Cadmium 50 mM 6 hrs, larvae L3    0 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    10 Cadmium 100 mM 48 hrs, 4-day adult    25 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    7 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    2 Ethanol 2.5% 3 hrs, larvae L3    29 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    19 Caffeine 25 mg/ml 48 hrs, 4-day adult    7 Paraquat 5 mM 48 hrs, 4-day adult    18 Paraquat 10 mM 48 hrs, 4-day adult    15 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_13 mE1_20_mRNA_expression_cluster_06
External Data & Images
Linkouts	BDGP expression data • SD02282 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0002948 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • S.CX001274;CG1763-RA FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for nod Stages(s) 4-6 Stages(s) 7-8 Stages(s) 9-10 Stages(s) 11-12 Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | male | cold sensitive nodDR4 lethal | recessive | cold sensitive nodDTW viable nodEY01772 nodf04008 viable, with Scer\GAL4pnr-MD237 nodGD16725 Sterility Allele fertile nodEY01772 nodf04008 Other Phenotypes Allele meiotic cell cycle defective nod4 meiotic cell cycle defective | dominant nodDR3 nodDTW meiotic cell cycle defective | dominant | female nodDTW meiotic cell cycle defective | female (with nod2) noda meiotic cell cycle defective | female (with noda) nod2 meiotic cell cycle defective | female | cold sensitive nodDTW meiotic cell cycle defective | recessive nod1 nod2 nod3 nod4 nod5 nod6 nodunspecified mitotic cell cycle defective nodDTW Phenotype manifest in Allele meiosis & nuclear chromosome & oocyte nod4 meiosis & nuclear chromosome | female nodDTW meiosis & nuclear chromosome | female (with nod2) noda meiosis & nuclear chromosome | female (with noda) nod2 mitosis & nuclear chromosome noddsRNA.cGa polytene chromosome chromocenter nodunspecified
Classical Alleles ( 19 )
For All Classical Alleles Show
Allele of nod Class Mutagen Stocks Known lesion nod4 amorphic allele - genetic evidence, loss of function allele gamma ray 2 Yes nod2 gamma ray spontaneous 2 Yes nod3 loss of function allele gamma ray 2 Yes nodDTW antimorphic allele - genetic evidence ethyl methanesulfonate 2 Yes nodf04008 piggyBac transposase 2 -- noda amorphic allele - genetic evidence, loss of function allele, hypomorphic allele - genetic evidence ethyl methanesulfonate 1 Yes node03334 piggyBac transposase 1 -- nodEY01772 P-element activity 1 -- nodf01431 piggyBac transposase 1 -- nod1 gamma ray 0 Yes nod5 loss of function allele gamma ray 0 -- nod6 loss of function allele gamma ray 0 Yes nod9 natural population 0 Yes nodA4579T natural population 0 Yes nodDR1 loss of function allele ethyl methanesulfonate 0 Yes nodDR2 loss of function allele ethyl methanesulfonate 0 Yes nodDR3 loss of function allele ethyl methanesulfonate 0 Yes nodDR4 ethyl methanesulfonate 0 Yes nodunspecified 0 --
Alleles Carried on Transgenic Constructs ( 19 )
For All Alleles Carried on Transgenic Constructs Show
Allele of nod Class Mutagen Stocks Known lesion nodGD16725 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes nodUbi-p63E.T:Hsap\MYC in vitro construct - regulatory fusion 1 Yes nodC.hs.T:Zzzz\FLAG in vitro construct - coding region fusion in vitro construct - regulatory fusion 0 Yes nodCN1.hs.T:Zzzz\FLAG in vitro construct - coding region fusion in vitro construct - regulatory fusion 0 Yes nodCN2.hs.T:Zzzz\FLAG in vitro construct - coding region fusion in vitro construct - regulatory fusion 0 Yes nodCN3.hs.T:Zzzz\FLAG in vitro construct - coding region fusion in vitro construct - regulatory fusion 0 Yes nodCN4.hs.T:Zzzz\FLAG in vitro construct - coding region fusion in vitro construct - regulatory fusion 0 Yes noddsRNA.cGa in vitro construct - RNAi 0 Yes nodGD8802 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes nodhs.T:Zzzz\FLAG in vitro construct - coding region fusion in vitro construct - regulatory fusion 0 Yes nodN.hs.T:Zzzz\FLAG in vitro construct - coding region fusion in vitro construct - regulatory fusion 0 Yes nodNDD1-2.Scer\UAS.P\T.T:Avic\GFP-EGFP in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - amino acid replacement in vitro construct - site directed mutagenesis 0 Yes nodNDD3.Scer\UAS.P\T.T:Avic\GFP-EGFP in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - amino acid replacement in vitro construct - site directed mutagenesis 0 Yes nodNDD4.Scer\UAS.P\T.T:Avic\GFP-EGFP in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - amino acid replacement in vitro construct - site directed mutagenesis 0 Yes nodNIG.1763R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes nodScer\UAS.cAa.T:Avic\GFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes nodScer\UAS.P\T.T:Avic\GFP-EGFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes nodScer\UAS.P\T.T:Avic\GFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes nodt4.0 in vitro construct - genomic fragment 0 Yes
Aneuploid Aberrations
Disrupted in	Df(1)52 (Meller and Rattner, 2002) Df(1)HA85 (Voelker et al., 1985, Cook et al., 1997) Df(1)m259-4 (Voelker et al., 1985) Df(1)N71 (Voelker et al., 1985, Rasooly et al., 1991) Df(1)v65b (Rasooly et al., 1991) Df(1)v-N48 (Voelker et al., 1985, Zhang et al., 1990)
Not disrupted in	Df(1)GA112 (Voelker et al., 1985, Zhang et al., 1990) Df(1)HM456 (Voelker et al., 1985) Df(1)RA37 (Voelker et al., 1985)
Not duplicated in	Dp(1;2)v+75d (Voelker et al., 1985) Dp(1;Y)v+y+ (Voelker et al., 1985)
Duplicated in	Dp(1;2)v+65b (Rasooly et al., 1991) Dp(1;3)v+74c (Rasooly et al., 1991) Dp(1;Y)y+v+#3 (Rasooly et al., 1991)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data heat-shock construct P{flag-Cnodhsp} NA P{flag-Nnodhsp} NA P{flag-nodhsp.CN1} NA P{flag-nodhsp.CN2} NA P{flag-nodhsp.CN3} NA P{flag-nodhsp.CN4} NA P{flag-nodhsp} NA UAS construct P{GD8802} NA P{GD16725} NA P{NIG.1763R} NA P{UAS-nod.GFP.A} NA P{UASp-nod-EGFP.NDD1-2} NA P{UASp-nod-EGFP.NDD3} NA P{UASp-nod-EGFP.NDD4} NA P{UASp-nod-EGFP} NA P{UASp-nod-GFP} NA characterization construct P{NZ} NA P{UAS-Khc::nod::lacZ} NA P{UAS-nod.GFP} NA P{UAS-nod.YFP} NA P{UASp-Khc::nod::lacZ} NA P{Ubi-p63E-nod.Myc} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EPgy2}nodEY01772 NA miscellaneous insertions 3S18{}nod2 NA PBac{RB}node03334 NA PBac{WH}nodf01431 NA PBac{WH}nodf04008 NA
Gene Ontology: Function, Process & Cellular Component ( 13 unique terms )
Terms Based on Experimental Evidence ( 8 terms )
Molecular Function
CV term Evidence References
microtubule motor activity inferred from mutant phenotype (assigned by UniProtKB) (Zhang et al., 1990) microtubule plus-end binding inferred from direct assay (Cui et al., 2005)
Biological Process
CV term Evidence References
distributive segregation inferred from mutant phenotype (Theurkauf and Hawley, 1992, Carpenter, 1973) establishment of meiotic spindle orientation inferred from mutant phenotype (Theurkauf and Hawley, 1992) meiotic chromosome segregation inferred from mutant phenotype (assigned by UniProtKB) (Zhang et al., 1990) mitotic spindle organization inferred from mutant phenotype (Goshima et al., 2007) positive regulation of microtubule polymerization inferred from direct assay (Cui et al., 2005) spindle assembly involved in female meiosis I inferred from mutant phenotype (Theurkauf and Hawley, 1992)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 6 terms )
Molecular Function
CV term Evidence References
ATP binding inferred from electronic annotation with InterPro:IPR001752, InterPro:IPR019821 (FlyBase Curators et al., 2004-) DNA binding inferred from electronic annotation with InterPro:IPR003583 (FlyBase Curators et al., 2004-) microtubule motor activity inferred from sequence or structural similarity (Goldstein and Gunawardena, 2000)
Biological Process
CV term Evidence References
DNA repair inferred from electronic annotation with InterPro:IPR003583 (FlyBase Curators et al., 2004-) microtubule-based movement inferred from electronic annotation with InterPro:IPR001752, InterPro:IPR019821 (FlyBase Curators et al., 2004-) inferred from sequence or structural similarity (Goldstein and Gunawardena, 2000)
Cellular Component
CV term Evidence References
kinesin complex inferred from sequence or structural similarity (Goldstein and Gunawardena, 2000) inferred from sequence or structural similarity with Khc (assigned by UniProtKB) (Zhang et al., 1990)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions nod allele Gene References nod2 lwr (Apionishev et al., 2001) ncd (Knowles and Hawley, 1991) neb (Ruden et al., 1997) nod3 ncd (Knowles and Hawley, 1991) neb (Ruden et al., 1997) αTub67C (Matthies et al., 1999) nod4 ncd (Knowles and Hawley, 1991) neb (Ruden et al., 1997) sub (Giunta et al., 2002) nod6 ncd (Knowles and Hawley, 1991) noda lwr (Apionishev et al., 2001) ncd (Knowles and Hawley, 1991) nodDTW lwr (Apionishev and Rasooly, 1997, Apionishev et al., 2001) mei-218 (Apionishev et al., 2001) mwr (Rasooly, 1996) neb (Ruden et al., 1997) nodunspecified αTub67C (Matthies et al., 1999)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 58516 DroID - A comprehensive database of gene and protein interactions. • FBgn0002948 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 32107
Orthologs
OrthoDB Orthologs (15) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6HDTDV Dipteran inclusive ortholog search EOG6KKZC3 Insect inclusive ortholog search EOG67M0TZ Arthropod inclusive ortholog search No orthologs identified Metazoa inclusive ortholog search No orthologs identified
Orthologs in Drosophila Species (EOG6HDTDV)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\nod     Drosophila simulans   Dsim\GD17032     Drosophila sechellia   Dsec\GM13095 Y   Drosophila erecta   Dere\GG18431 Y   Drosophila yakuba   Dyak\GE15949 Y   Drosophila ananassae   Dana\GF22525 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA14585 Y   Drosophila persimilis   Dper\GL26998 Y   Drosophila willistoni   Dwil\GK25234 Y   Drosophila virilis   Dvir\GJ19420 Y   Drosophila mojavensis   Dmoj\GI14606 Y
Orthologs in non-Drosophila Dipterans (EOG6KKZC3)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL005497   Aedes aegypti Yellow fever mosquito  Aaeg\AAEL004241   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ019631
Orthologs in non-Dipteran Insects (EOG67M0TZ)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Tribolium castaneum Red flour beetle  Tcas\TC003812
Orthologs in non-Insect Arthropods (None identified)
No non-Insect Arthropod orthologies identified
Orthologs in non-Arthropod Metazoa (None identified)
No non-Arthropod Metazoa orthologies identified
Human Orthologs (0)
Gene OMIM HGNC
AAA Orthologs (9) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila sechellia Dsec\GM13095 Drosophila erecta Dere\GG18431 Drosophila yakuba Dyak\GE15949 Drosophila ananassae Dana\GF22525 Drosophila pseudoobscura pseudoobscura Dpse\GA14585 Drosophila persimilis Dper\GL26998 Drosophila willistoni Dwil\GK25234 Drosophila virilis Dvir\GJ19420 Drosophila mojavensis Dmoj\GI14606
Stocks & Reagents
Stocks Listed in FlyBase ( 17 )
Bloomington	18724 w1118 PBac{WH}nodf04008 32010 y1 w*; P{Ubi-p63E-nod.Myc}2 20065 y1 w67c23 P{EPgy2}nodEY01772 3430 FM7a, nod4/C(1)DX/Dp(1;Y)y+; svspa-pol 3432 nodDTW f1/Dp(1;Y)BS/C(1)DX, y1 f1
Harvard	- PBac{RB}node03334 - PBac{WH}nodf01431 - PBac{WH}nodf04008
Kyoto	107039 nodDTW f1/Dp(1;Y)BS/C(1)DX, y1 f1 106771 FM7a, nod2/Dp(1;Y)y+/C(1)DX, y1 f1; svspa-pol
VDRC	v48150 w1118; P{GD16725}v48150 v48148 w1118; P{GD16725}v48148/TM3
	More stocks available...
Genomic Clones ( 1 )
	BACR26B05 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 79 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	SD02282
Other clones	MIP02859
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GM03690 LD03973 LD31089 RE07916 RE19552 RE53577 RH40016 SD11570
Other clones	10653 28086 43404 63577 64762 70188 123378 149403 158419 181092 190991 CK01.49A.A4 CK01.168A.F1 CK01502 Dmel_dig_014797_0618_1603 EK102916 EK113664 EK135762 EK162941 EK190347 EK192810 EK197036 EK246326 EK254444 EK286020 EN00603 IP12981 RH02444 RP003004240 RP003006157 RP003048263 RP003056186 RP003066216 RP003072079 RP003078858 RP003095307 RP003100563 RP003129355 RP003187773 RP003215079 RP003219929 RP003236673 RP003238842 RP003242420 RP003242835 RP003257414 RP003300417 RP003337194 RP003339455 RP003340736 RP003343331 RP003387052 RP003403706 RP003428300 RP003439012 RP003446014 RP003448269 RP003457094 RP003457482 RP003457719 RP003469462 RP003500076 RP003530746 RP003553142 RP003622816 RP003635708 RP003635914 RP003636641 RP003653418
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0002948 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 32107
Antibody Information
	polyclonal (Cane et al., 2013)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: nod CG1763 (Zhong, 1999.11)
Source for database merge of
Additional comments
Other Comments
	RNAi screen using dsRNA made from templates generated with primers directed against this gene results in chromosome misalignment on the metaphase spindle when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA. (Goshima et al., 2007) dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003) nod, a female-specific gene expressed in the ovary, is closely linked to roX2, a gene encoding an RNA with no apparent reading frame that shows male-specific expression in the adult fly. (Amrein and Axel, 1997) The reciprocal location of nod and Khc fusion proteins indicates microtubule polarity in the oocyte, epithelium, neuron and muscle. (Clark et al., 1997) nod is necessary for chromosome segregation and can interact specifically with the centromere. nod requires extracentromeric regions for its action in chromosome segregation. (Cook et al., 1997) Examination of meiotic prophase in nod mutants reveals that while heterochromatic pairing may be a component of the mechanism of homologous segregation, it is not sufficient to guarantee proper disjunction of nonexchange homologs. (Dernburg et al., 1996) The nonmotor domain of the nod protein can mediate direct binding to DNA. During prometaphase nod protein is localized on oocyte chromosomes and is not restricted to either specific chromosomal regions or to the kinetochore. Thus motor-based chromosome-microtubule interactions are not limited to the centromere but extend along the chromosome arms, providing a molecular explanation for the polar ejection force. (Afshar et al., 1995) An 82 amino acid residue segment of the tail of the nod protein is necessary for the localisation of the protein onto chromosomes. (Afshar et al., 1995) nod is required for the correct segregation of the non-exchange chromosomes during meiosis, mutations result in a high level of nondisjunction of the non-exchange fourth chromosomes and achiasmate X chromosomes. (Afshar et al., 1995) Transmission of the Dp(1;f)1187 minichromosome is sensitive to the dosage of nod+. Multiple regions of Dp(1;f)1187 interact with nod+ to promote normal chromosome transmission. Most nod+ interactions are observed with regions that are not essential for centromere function. (Murphy and Karpen, 1995) The sequence of the nod protein has been compared with the sequences of a variety of kinesin family proteins. (Goodson et al., 1994) Mutations at nod do not disrupt meiotic spindle formation and show no interaction with Axs. (Whyte et al., 1993) nod protein is a member of the kinesin superfamily so it is proposed that the nod locus encodes a spindle motor that is required to hold distributively paired chromosomes at the metaphase plate until anaphase. (Zhang et al., 1990) nod gene product is required for nonexchange chromosomes. (Zhang and Hawley, 1990) Females homozygous for nod alleles exhibit high frequencies of meiotic chromosome loss and nondisjunction at meiosis I. Most nod-induced nondisjunctional events involve nonexchange chromosomes. For example, in nod/nod females nondisjunction frequencies for the always nonexchange fourth chromosomes approaches 90% (the vast majority of gametes are nullo-4 ova), whereas nonexchange X chromosomes apparently disjoin at random. Both the frequency of exchange and the disjunction of exchange bivalents was shown to be normal in nod/nod females. Thus, with respect to its role in meiosis, the nod+ function appears to be limited to the distributive segregation system. Based on an analysis of secondary nondisjunction in noda/noda females, Carpenter concluded that the nod defect does not impair the process of partner choice within the distributive system, but rather specifically impairs the disjunctional process. Nonexchange chromosomes derived from noda/noda mothers also undergo nondisjunction and presumably loss, at meiosis II. In addition, chromosomes derived from noda/noda mothers are mitotically unstable. nod-induced mitotic chromosome loss is restricted to maternal nonexchange chromosomes and does not exert any discernible effect on meiosis in males or on mitotic chromosome stability (Baker, Carpenter and Ripoll, 1978). Although none of the existing nod alleles is lethal or female sterile, the dosage-sensitive antimorphic mutation l(1)TW6 (Wright, 1973) is argued to be allelic to nod on the basis of three lines of evidence. First, l(1)TW6/+ females display a meiotic phenotype that is virtually identical to that exhibited by noda/noda females. Second, the two loci map to the same position on the X chromosome (Wright, 1973; Baker). Third, a γ-ray induced revertant of l(1)TW6 was shown to be a recessive nod allele (New and Hawley).
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_078570 RefSeq (Proteins) • NP_511125 DDBJ / EMBL / GenBank • AAA28653 AAC14452 AAF48064 AAK84937 AI531306 AW944230 AY050238 CL706558 CZ481108 CZ481109 CZ483901 M36195 M94188 Entrez Gene - A searchable database of RefSeq genes. • 32107 UniProtKB/Swiss-Prot • P18105
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Kinesin, motor domain (IPR001752) Helix-hairpin-helix DNA-binding motif, class 1 (IPR003583) Kinesin, motor region, conserved site (IPR019821) P-loop containing nucleoside triphosphate hydrolase (IPR027417) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • SD02282
Linkouts
	BioGRID - A database of protein and genetic interactions • 58516 DroID - A comprehensive database of gene and protein interactions. • FBgn0002948 DRSC - Results from RNAi screens. • FBgn0002948 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0002948 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • S.CX001274;CG1763-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0002948 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 32107 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 32107 modMine - Data generated by the modENCODE project. • FBgn0002948
Synonyms & Secondary IDs ( 13 )
Reported As
Symbol Synonym	CG1763 (Gilliland, 2003.1.7, Goldstein and Gunawardena, 2000, Goshima et al., 2007, Yano et al., 2012) DmNod (Moore and Endow, 1996, Dagenbach and Endow, 2004, Hirokawa, 2012) DnMod (Kim and Endow, 2000) KIF 22 (Miki et al., 2001) KIF22 (Hirokawa, 2012.11.20) l(1)TW6 (Rasooly et al., 1991, Ripoll et al., 1987, ) Nod (Goshima and Vale, 2003, Cleveland et al., 2003, Cui and Hawley, 2003, Goodson et al., 1994, Huo et al., 2007, Goshima et al., 2007, Cui et al., 2005, Yang et al., 2008, Cochran et al., 2011, Miki et al., 2005, Miki et al., 2001, Hirokawa, 2012.11.20) nod (Mukai et al., 2006, Theurkauf and Hawley, 1992, Carpenter, 1973, Brunet and Vernos, 2001, Apionishev et al., 2001, Wu et al., 2008, Zheng et al., 2008, Hartwig et al., 2008, Hughes et al., 2009, Straub et al., 2008, Menon and Meller, 2009, Bolivar et al., 2006, Hawley et al., 1994, Goldstein, 2010.9.10, Chubykin, 2001, Korenjak et al., 2012, Japanese National Institute of Genetics, 2012.5.21) NOD (Cane et al., 2013, Lawrence et al., 2002, Cochran et al., 2009)
Name Synonym	kinesin-10 (Cochran et al., 2011) no distributive disjunction (Mukai et al., 2006, Carpenter, 1973) No distributive disjunction (Yano et al., 2012) No Distributive Disjunction (Hirokawa, 2012.11.20)
Secondary FlyBase IDs
References ( 178 )
Generate a list of	All references relating to this gene ( 178 )
List References by type	Research paper  ( 82 ) Supplementary material  ( 9 ) Review  ( 40 ) Personal communication to FlyBase  ( 11 ) Abstract  ( 28 ) FlyBase analysis  ( 1 ) Stock list  ( 1 ) Website  ( 2 ) Curated genome annotation  ( 2 ) Protein sequence record  ( 1 )
Recent research papers ( 6 )
	Cane et al., 2013, J. Cell Biol. 200(2): 203--218 Elevated polar ejection forces stabilize kinetochore-microtubule attachments. [FBrf0220555] Gillies et al., 2013, Genetics 193(2): 443--451 Nondisjunctional segregations in Drosophila female meiosis I are preceded by homolog malorientation at metaphase arrest. [FBrf0220742] Collins et al., 2012, G3 (Bethesda) 2(11): 1369--1377 A Germline Clone Screen on the X Chromosome Reveals Novel Meiotic Mutants in Drosophila melanogaster. [FBrf0219963] Korenjak et al., 2012, Mol. Cell. Biol. 32(21): 4375--4387 RBF Binding to both Canonical E2F Targets and Noncanonical Targets Depends on Functional dE2F/dDP Complexes. [FBrf0219643] Yano et al., 2012, J. Neurogenet. 26(1): 53--63 Identification of proteasome components required for apical localization of chaoptin using functional genomics. [FBrf0217843] Cochran et al., 2011, Nat. Struct. Mol. Biol. 19(1): 122--127 A metal switch for controlling the activity of molecular motor proteins. [FBrf0217143] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...