Expression was examined at four phases of embryonic stage 5. The striped pattern becomes visible in phase 1 (0-5'), all stripes except stripe 7 are expressed during phase 2 (5-17'), and their spacing and expression levels become largely uniform by phase 3 (17-30'). The stripes initially appear less clearly separated and more graded.

In stage 11 embryos, odd is expressed in stripes that are adjacent and posterior to hh-expressing cells in each parasegment. It is also expressed in the presumptive foregut.

Expression assayed at stages 9, 11, 13, and 17. Expression may be continuous between assayed stages in some tissues.

The odd, sob and drm transcripts are detected an identical pattern in the third instar leg disc. There is segmental expression manifested as concentric rings on the distal edges of each presumptive leg segment not including the 4 tarsal segments (i.e. 5 rings of expression). There is an additional area of expression in the center of the disc that may correspond to a track of glial cells. The expression of drm is observed to begin during early larval development as a proximal ring of expression corresponding to the coxa. As larval development proceeds additional rings of expression are observed until the full complement of five rings is observed. Expression of odd, sob and drm occur in cells that are adjacent and distal to cells expressing the N ligands, Dl and Ser.

At the cellular blastoderm stage, odd transcripts accumulate in 7 transverse stripes that are 3-4 cells wide and correspond to the odd-numbered segments. A domain of expression is also observed at 85-100% egg length. At around the time of gastrulation, the stripe pattern changes to a segment polarity pattern of 14 stripes. The odd stripes are strongest in the blastoderm stage and diminish intensity when they are expressed in the segment polarity pattern. The odd expression pattern overlaps that of sob at these stages. In later stages, odd continues to be expressed in the epidermis but is also expressed in the proventriculus, proximal Malpighian tubules, brain, pharyngeal muscles, ventral nerve cord, pericardial cells, and garland cells.

odd transcription is repressed by ectopic eve protein in eve^hs.PS embryos. Timing suggests that eve is a direct regulator of odd.

odd transcripts are first detected in the syncytial blastoderm in a 5 cell wide stripe at 62% egg length. During cellularization, the pattern evolves into one of six regular stripes of ~3-4 cell widths. There is a noticeable posterior and dorsal lag in the generation of these stripes. A non-periodic anterior domain of expression is also observed which persists through subsequent stages. Labelling with ftz indicates that the resolution of the odd pattern into six stripes is coincident with the appearance of the seven stripe ftz pattern. A 7th posteriormost odd stripe appears at 15% egg length after nuclear elongation but before the completion of cellularization. During gastrulation, this pattern evolves into a pattern in which odd is expressed in homologous portions of each segment. This occurs through the narrowing of the original seven stripes and the appearance of 8 new stripes. Results of labelling with en suggest that each of the seven odd stripes lies posterior to an even-numbered en stripe.

Around 90% of odd positive adult neurons are positive for Scer\GAL4^ChAT.7.4.

In early third instar larvae, the highest levels of odd are observed in medullary zone prohemocytes in the lymph gland. At this time odd is downregulated in terminally differentiated plasmatocytes. This epression pattern is maintained until the late-third larval instar. odd is expressed in prohemocytes throughout lymph gland development and maturation and is downregulated as pro-hemocytes differentiate.

odd protein is expressed in the embryonic lymph gland.

Expression is observed in A6 to A9 dMP2 neurons.

odd protein is expressed in the developing embryonic brain from embryonic stage 11, and is expressed in three discrete clusters at the end of embryogenesis. It is also expressed in a few neuroblasts and neurons per segment in the ventral nerve cord. odd protein is transiently expressed in a small subset of midline glial cells at embryonic stage 9. At embryonic stage 16, odd protein is expressed in a ring of cells encircling the salivary gland duct about midway along its length. This ring is derived from six odd-positive cells in the ventral epidermis of parasegment 2 at embryonic stage 11; these cells reorganize to form a cluster by stage 12, then migrate anteriorly and dorsally to their final position.

odd protein is expressed in two rows of mesodermal cells flanking the dorsal midline of late stage embryos. These cells are physically close to, and flank, the cardioblasts. Pericardial cells expressing odd protein do not express either eve or tin, which are expressed in all previously identified pericardial cells; they do however also express zfh1. odd-expressing pericardial cells lie lateral to tin-expressing pericardial cells