Expression was examined at four phases of embryonic stage 5. The striped pattern becomes visible in phase 1 (0-5'), all stripes except stripe 7 are expressed during phase 2 (5-17'), and their spacing and expression levels become largely uniform by phase 3 (17-30'). The stripes initially appear less clearly separated and more graded.
In stage 11 embryos, odd
is expressed in stripes that are adjacent and posterior to hh
-expressing cells in each parasegment. It is also expressed in the presumptive foregut.
Expression assayed at stages 9, 11, 13, and 17. Expression may be continuous between assayed stages in some tissues.
transcripts are detected an identical pattern in the third instar leg disc. There is segmental expression manifested as concentric rings on the distal edges of each presumptive leg segment not including the 4 tarsal segments (i.e. 5 rings of expression). There is an additional area of expression in the center of the disc that may correspond to a track of glial cells. The expression of drm
is observed to begin during early larval development as a proximal ring of expression corresponding to the coxa. As larval development proceeds additional rings of expression are observed until the full complement of five rings is observed. Expression of odd
occur in cells that are adjacent and distal to cells expressing the N
At the cellular blastoderm stage, odd
transcripts accumulate in 7 transverse stripes that are 3-4 cells wide and correspond to the odd-numbered segments. A domain of expression is also observed at 85-100% egg length. At around the time of gastrulation, the stripe pattern changes to a segment polarity pattern of 14 stripes. The odd
stripes are strongest in the blastoderm stage and diminish intensity when they are expressed in the segment polarity pattern. The odd
expression pattern overlaps that of sob
at these stages. In later stages, odd
continues to be expressed in the epidermis but is also expressed in the proventriculus, proximal Malpighian tubules, brain, pharyngeal muscles, ventral nerve cord, pericardial cells, and garland cells.
transcription is repressed by ectopic eve
protein in evehs.PS
embryos. Timing suggests that eve
is a direct regulator of odd
transcripts are first detected in the syncytial blastoderm in a 5 cell wide stripe at 62% egg length. During cellularization, the pattern evolves into one of six regular stripes of ~3-4 cell widths. There is a noticeable posterior and dorsal lag in the generation of these stripes. A non-periodic anterior domain of expression is also observed which persists through subsequent stages. Labelling with ftz
indicates that the resolution of the odd
pattern into six stripes is coincident with the appearance of the seven stripe ftz
pattern. A 7th posteriormost odd
stripe appears at 15% egg length after nuclear elongation but before the completion of cellularization. During gastrulation, this pattern evolves into a pattern in which odd
is expressed in homologous portions of each segment. This occurs through the narrowing of the original seven stripes and the appearance of 8 new stripes. Results of labelling with en
suggest that each of the seven odd
stripes lies posterior to an even-numbered en