• Home
• Tools
  • Tools Overview
  • Query Tools

    
    • QuickSearch
    • QueryBuilder
    • TermLink
    • CytoSearch
    • Interactions Browser
    • ImageBrowse
    • Find A Person
    • Google™ FlyBase
  • Genomic/Map Tools

    
    • BLAST
    • GBrowse
    • CytoSearch
    • Aberrations Maps
    • Chromosome Maps
    • Coordinates Converter
  • Retrieve/Convert

    
    • Batch Download
    • Coordinates Converter
    • ID Converter
  • Data Submission

    
    • Fast-Track Your Paper
    • Submit Personal Comm
    • Add A New Person
    • Update Person Info
  • People

    
    • Find A Person
    • Add A New Person
    • Update Person Info
• Files
  • Files Overview
  • Downloads:
  • Precomputed files
  • Archived data
  • Releases (FTP)
  • Genomes (FTP)
  • AAA data (FTP)
  • Lists:
  • Transposons (Dmel)
  • Transposons (other)
  • Vectors & Constructs
  • Anatomical Terms
  • Map Conversion
• Species
  • Phylogeny
  • Sequenced Species
  • Synteny Table
  • Color Illustrations
  • Abbreviations
• Documents
  • About FlyBase
  • Reference Manual:
  • Sections
  • Contents
  • Nomenclature
  • Release Notes
• Resources
  • All Resources
  • Model Organisms
  • Stock Collections
  • Other links:
  • BDGP
  • DGRC
  • FlyExpress
  • Interactive Fly
  • modENCODE
  • Textpresso for Fly
• News
  • News
  • FlyBase Forum
  • FlyBase Positions
  • Meetings
  • Courses
  • Fly Board
  • White papers
  • Funding
  • bionet.dros
• Help
  • Google™ FlyBase
  • Site Map
  • FAQs
  • FlyBase Guides
  • Video Tutorials
  • Report help
  • New to Flies
  • Pers. comm.
  • Author guidelines
  • Contact FlyBase
• Archives
  • Prior Release
  • Other Archives
  • Coordinate Converter

Gene Dmel\Ote

General Information
Symbol	Dmel\Ote	Species	D. melanogaster
Name	Otefin	Annotation symbol	CG5581
Feature type	protein_coding_gene	FlyBase ID	FBgn0003022
Gene Model Status	Current	Stock availability	3 publicly available
Genomic Location
Chromosome (arm)	2R	Recombination map
Cytogenetic map	55C2-55C2	Sequence location	2R:14,163,112..14,164,698 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Otefin is referred to in FlyBase by the symbol Dmel\Ote (CG5581, FBgn0003022). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: protein binding; transcription factor binding; transcription corepressor activity. There is experimental evidence that it is involved in the biological process: germ-line stem cell maintenance; nuclear envelope reassembly; positive regulation of BMP signaling pathway; female germ-line stem cell division; negative regulation of transcription, DNA-dependent. 14 alleles are reported. The phenotypes of these alleles are annotated with: ovary; female germline stem cell. It has one annotated transcript and one annotated polypeptide. Protein features are: LEM-like domain; Lamino-associated polypeptide 2/emerin. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of low expression. Peak expression observed within 00-06 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult ovary. Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system, larval trachea. Comments on Affy2 ProbeSet: ProbeSet 1631973_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of Ote. Gene sequence location is 2R:14163112..14164698.
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2012_01	Sequence features HFA07498 BKN29866
FB2011_10	References Herranz et al., 2010, Mol. Ecol. 19(19): 4255--4264
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 55C2-55C2   Limits computationally determined from genome sequence between P{PZ}Hsf03091&P{EP}Dgp-1EP731 and P{PZ}sbb04525&P{EP}EP1081EP1081
Experimentally Determined Cytological Location
Cytogenetic map Notes References 55C-55C   (determined by in situ hybridisation)   (Padan et al., 1990)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Ote for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.39
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Ote-RA FBtr0086768  NM_057316   1587   424
Additional Transcript Data & Comments
Reported size (kB)	1.6 (northern blot) (Padan et al., 1990)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Ote-PA   FBpp0085947   46.6   424   9.99     NP_476664   AAF57722
Additional Polypeptide Data & Comments
Reported size (kDa)	406 (aa); 45 (kD predicted) (Padan et al., 1990) 53 (kD observed) (Harel et al., 1989)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Lamino-associated polypeptide 2/emerin (IPR003887) LEM-like domain (IPR011015)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA141918   AA391446   AA695978   AA941975   AE013599 AAF57722   AC004295   AC004309   AI511750   AI516149   AI516401   AI516620   AI519885   AW943586   AY051940 AAK93364   BF492722   BF492723   BI165863   BI353061   BI355228   BI365285   BI373070   BI486966   BI578584   BI578613   BI640808   CO190324   CO262882   CO281288   CO286556   CO310950   CO311519   EC206765   EC218265   EC219497   EC225181   EC226266   EC235274   EC245184   EV584304   GH747635   GH747687   GH750164   GH753901   GH756898   GH757325   GH758591   GH770352   GH770526   GH770963   GH773387   GH775853   GH776908   GH780983   GH781148   GH781864   GH785118   GH786353   GH787340   GH789334   GH789879   GH792417   GH794890   GH795425   GH795787   GH796343   GH798242   GH800097   GH800868   GH801178   GH802372   GH805077   GH805425   GH805577   GH806456   GH811774   GH813273   GH813532   GH816215   GH817400   GH818364   GH820956   GH822403   GH822696   GH822841   GH823344   GH824301   GH824740   GH828375   GH829114   GH830073   GH830545   GH833574   GH833650   GH834822   GH836547   GH838824   GH842357   GH842507   GH844228   GH844745   GH845314   GH849411   GH851767   GH852674   GH854206   GH854974   GH856401   GH857815   GH859627   GH859971   GH861120   GH861364   GH861448   GH862591   GH863872   GH866577   GH866802   GH867337   GH870848   GH871271   GH874044   GH875348   GH876521   GH877561   GH879031   GH880348   GH880370   GH880836   GH881987   GH882208   GH883006   GH885021   GH888964   GH889087   GH890626   GH891911   GH893091   GH896332   GH896813   GH897151   GH898560   GH899002   GH899149   GH902354   GH902920   GH903407   GH903939   GH904616   GH904852   GH905129   GH906572   GH906783   GH907059   GH907243   GH907772   GH912004   GH912141   GH914536   GH915443   GH915456   GH915589   GH916036   GH917185   GH918202   GH919755   GH921801   GH922416   GH924720   GH924829   GH926676   GH926818   GH926947   GH927672   GH929086   GH929478   GH930153   GH932434   GH934131   GH936063   GH937407   GH937614   GH939347   GH939844   GH941006   X17495 CAA35530
UniProtKB/Swiss-Prot	P20240
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
northern blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage -- adult stage     (Padan et al., 1990)
Additional Descriptive Data
	Ote transcript is present in all developmental stages. It is more abundant in embryos and in pupae. (Padan et al., 1990)
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
immunolocalization Stage Tissue/Position (including subcellular localization) Reference embryonic stage | early     (Harel et al., 1989) embryonic stage & pupal stage     (Harel et al., 1989) embryonic stage -- adult stage     (Ashery-Padan et al., 1997) oogenesis     (Ashery-Padan et al., 1997)
Additional Descriptive Data
	Ote protein is detected at high levels in embryos and during pupation. In embryos and tissue culture cells, Ote protein localizes to the periphery of the nucleus. (Harel et al., 1989) Between stages S4 and S6-S7 of oogenesis, Ote protein localizes to the nuclear envelope of nurse cells, follicle cells and oocytes, but after stage S6-S7, nucleoplasmic and cytoplasmic accumulation is also observed. During embryogenesis, Ote protein is associated with the nucleus as well as with nonnuclear membrane vesicles. Like Lam protein, Ote protein is detected in the nuclei of most cells during larval, pupal and adult stages. Neither protein is detected in stages 6-11 of spermiogenesis. In larval and adult tissues, Ote pr tein localizes to the nuclear envelope. (Ashery-Padan et al., 1997) Using EM immunogold labeling, Ote protein was localized to the nucleoplasmic side of the inner nuclear membrane. (Ashery-Padan et al., 1997)
Marker for
Subcellular Localization
CV Term	nuclear inner membrane (Ashery-Padan et al., 1997) nuclear envelope cytoplasm nucleoplasm (Ashery-Padan et al., 1997)
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0003022    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0003022 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of low expression. Peak expression observed within 00-06 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0003022 expression level Developmental Stage   Expression Level embryo 00-02hr    11968 embryo 02-04hr    7061 embryo 04-06hr    7007 embryo 06-08hr    5870 embryo 08-10hr    3674 embryo 10-12hr    1942 embryo 12-14hr    997 embryo 14-16hr    562 embryo 16-18hr    332 embryo 18-20hr    320 embryo 20-22hr    290 embryo 22-24hr    294 larva L1    345 larva L2    470 larva L3 12hr old    498 larva L3 puffstage 1-2    992 larva L3 puffstage 3-6    1272 larva L3 puffstage 7-9    1308 white prepupae new    1239 white prepupae 12hr    2184 white prepupae 24hr    1581 pupae 2d postWPP    632 pupae 3d postWPP    349 pupae 4d postWPP    376 adult male 01day    333 adult male 05day    613 adult male 30day    390 adult female 01day    2060 adult female 05day    4868 adult female 30day    5352 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (11968) embryo 02-04hr  (7061) embryo 04-06hr  (7007) embryo 06-08hr  (5870) embryo 08-10hr  (3674) embryo 10-12hr  (1942) embryo 12-14hr  (997) embryo 14-16hr  (562) embryo 16-18hr  (332) embryo 18-20hr  (320) embryo 20-22hr  (290) embryo 22-24hr  (294) larva L1  (345) larva L2  (470) larva L3 12hr old  (498) larva L3 puffstage 1-2  (992) larva L3 puffstage 3-6  (1272) larva L3 puffstage 7-9  (1308) white prepupae new  (1239) white prepupae 12hr  (2184) white prepupae 24hr  (1581) pupae 2d postWPP  (632) pupae 3d postWPP  (349) pupae 4d postWPP  (376) adult male 01day  (333) adult male 05day  (613) adult male 30day  (390) adult female 01day  (2060) adult female 05day  (4868) adult female 30day  (5352) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (11968) embryo 02-04hr  (7061) embryo 04-06hr  (7007) embryo 06-08hr  (5870) embryo 08-10hr  (3674) embryo 10-12hr  (1942) embryo 12-14hr    997 embryo 14-16hr    562 embryo 16-18hr    332 embryo 18-20hr    320 embryo 20-22hr    290 embryo 22-24hr    294 larva L1    345 larva L2    470 larva L3 12hr old    498 larva L3 puffstage 1-2    992 larva L3 puffstage 3-6    1272 larva L3 puffstage 7-9    1308 white prepupae new    1239 white prepupae 12hr  (2184) white prepupae 24hr  (1581) pupae 2d postWPP    632 pupae 3d postWPP    349 pupae 4d postWPP    376 adult male 01day    333 adult male 05day    613 adult male 30day    390 adult female 01day  (2060) adult female 05day  (4868) adult female 30day  (5352) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr  (11968) embryo 02-04hr    7061 embryo 04-06hr    7007 embryo 06-08hr    5870 embryo 08-10hr    3674 embryo 10-12hr    1942 embryo 12-14hr    997 embryo 14-16hr    562 embryo 16-18hr    332 embryo 18-20hr    320 embryo 20-22hr    290 embryo 22-24hr    294 larva L1    345 larva L2    470 larva L3 12hr old    498 larva L3 puffstage 1-2    992 larva L3 puffstage 3-6    1272 larva L3 puffstage 7-9    1308 white prepupae new    1239 white prepupae 12hr    2184 white prepupae 24hr    1581 pupae 2d postWPP    632 pupae 3d postWPP    349 pupae 4d postWPP    376 adult male 01day    333 adult male 05day    613 adult male 30day    390 adult female 01day    2060 adult female 05day    4868 adult female 30day    5352 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    11968 embryo 02-04hr    7061 embryo 04-06hr    7007 embryo 06-08hr    5870 embryo 08-10hr    3674 embryo 10-12hr    1942 embryo 12-14hr    997 embryo 14-16hr    562 embryo 16-18hr    332 embryo 18-20hr    320 embryo 20-22hr    290 embryo 22-24hr    294 larva L1    345 larva L2    470 larva L3 12hr old    498 larva L3 puffstage 1-2    992 larva L3 puffstage 3-6    1272 larva L3 puffstage 7-9    1308 white prepupae new    1239 white prepupae 12hr    2184 white prepupae 24hr    1581 pupae 2d postWPP    632 pupae 3d postWPP    349 pupae 4d postWPP    376 adult male 01day    333 adult male 05day    613 adult male 30day    390 adult female 01day    2060 adult female 05day    4868 adult female 30day    5352 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0003022 expression level Developmental Stage   Expression Level embryo 00-02hr    11968 embryo 02-04hr    7061 embryo 04-06hr    7007 embryo 06-08hr    5870 embryo 08-10hr    3674 embryo 10-12hr    1942 embryo 12-14hr    997 embryo 14-16hr    562 embryo 16-18hr    332 embryo 18-20hr    320 embryo 20-22hr    290 embryo 22-24hr    294 larva L1    345 larva L2    470 larva L3 12hr old    498 larva L3 puffstage 1-2    992 larva L3 puffstage 3-6    1272 larva L3 puffstage 7-9    1308 white prepupae new    1239 white prepupae 12hr    2184 white prepupae 24hr    1581 pupae 2d postWPP    632 pupae 3d postWPP    349 pupae 4d postWPP    376 adult male 01day    333 adult male 05day    613 adult male 30day    390 adult female 01day    2060 adult female 05day    4868 adult female 30day    5352 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (11968) embryo 02-04hr  (7061) embryo 04-06hr  (7007) embryo 06-08hr  (5870) embryo 08-10hr  (3674) embryo 10-12hr  (1942) embryo 12-14hr  (997) embryo 14-16hr  (562) embryo 16-18hr  (332) embryo 18-20hr  (320) embryo 20-22hr  (290) embryo 22-24hr  (294) larva L1  (345) larva L2  (470) larva L3 12hr old  (498) larva L3 puffstage 1-2  (992) larva L3 puffstage 3-6  (1272) larva L3 puffstage 7-9  (1308) white prepupae new  (1239) white prepupae 12hr  (2184) white prepupae 24hr  (1581) pupae 2d postWPP  (632) pupae 3d postWPP  (349) pupae 4d postWPP  (376) adult male 01day  (333) adult male 05day  (613) adult male 30day  (390) adult female 01day  (2060) adult female 05day  (4868) adult female 30day  (5352) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (11968) embryo 02-04hr  (7061) embryo 04-06hr  (7007) embryo 06-08hr  (5870) embryo 08-10hr  (3674) embryo 10-12hr  1942 embryo 12-14hr    997 embryo 14-16hr    562 embryo 16-18hr    332 embryo 18-20hr    320 embryo 20-22hr    290 embryo 22-24hr    294 larva L1    345 larva L2    470 larva L3 12hr old    498 larva L3 puffstage 1-2    992 larva L3 puffstage 3-6    1272 larva L3 puffstage 7-9    1308 white prepupae new    1239 white prepupae 12hr  2184 white prepupae 24hr  1581 pupae 2d postWPP    632 pupae 3d postWPP    349 pupae 4d postWPP    376 adult male 01day    333 adult male 05day    613 adult male 30day    390 adult female 01day  2060 adult female 05day  (4868) adult female 30day  (5352) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr  11968 embryo 02-04hr    7061 embryo 04-06hr    7007 embryo 06-08hr    5870 embryo 08-10hr    3674 embryo 10-12hr    1942 embryo 12-14hr    997 embryo 14-16hr    562 embryo 16-18hr    332 embryo 18-20hr    320 embryo 20-22hr    290 embryo 22-24hr    294 larva L1    345 larva L2    470 larva L3 12hr old    498 larva L3 puffstage 1-2    992 larva L3 puffstage 3-6    1272 larva L3 puffstage 7-9    1308 white prepupae new    1239 white prepupae 12hr    2184 white prepupae 24hr    1581 pupae 2d postWPP    632 pupae 3d postWPP    349 pupae 4d postWPP    376 adult male 01day    333 adult male 05day    613 adult male 30day    390 adult female 01day    2060 adult female 05day    4868 adult female 30day    5352 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    11968 embryo 02-04hr    7061 embryo 04-06hr    7007 embryo 06-08hr    5870 embryo 08-10hr    3674 embryo 10-12hr    1942 embryo 12-14hr    997 embryo 14-16hr    562 embryo 16-18hr    332 embryo 18-20hr    320 embryo 20-22hr    290 embryo 22-24hr    294 larva L1    345 larva L2    470 larva L3 12hr old    498 larva L3 puffstage 1-2    992 larva L3 puffstage 3-6    1272 larva L3 puffstage 7-9    1308 white prepupae new    1239 white prepupae 12hr    2184 white prepupae 24hr    1581 pupae 2d postWPP    632 pupae 3d postWPP    349 pupae 4d postWPP    376 adult male 01day    333 adult male 05day    613 adult male 30day    390 adult female 01day    2060 adult female 05day    4868 adult female 30day    5352 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0003022    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0003022 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult ovary. Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system, larval trachea. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0003022 expression level Tissue   Expression Level Larval Central Nervous System    465.3 Larval Midgut    29.1 Larval Hindgut    39.4 Larval Malpighian Tubules    22.1 Larval Fat Body    52.3 Larval Salivary Gland no informative data Larval Trachea    158.9 Larval Carcass    73.625 Adult Head    13.8 Adult Eye    13.825 Adult Brain    18.1 Adult Thoracic-Abdominal Ganglion    20.3 Adult Crop    17.5 Adult Midgut no informative data Adult Hindgut no informative data Adult Malpighian Tubules    9.6 Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart no informative data Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca no informative data Adult Ovary    581.8 Adult Testis    99.8 Adult Male Accessory Gland no informative data Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    465.3 Larval Midgut    29.1 Larval Hindgut    39.4 Larval Malpighian Tubules    22.1 Larval Fat Body    52.3 Larval Salivary Gland no informative data Larval Trachea    158.9 Larval Carcass    73.625 Adult Head    13.8 Adult Eye    13.825 Adult Brain    18.1 Adult Thoracic-Abdominal Ganglion    20.3 Adult Crop    17.5 Adult Midgut no informative data Adult Hindgut no informative data Adult Malpighian Tubules    9.6 Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart no informative data Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca no informative data Adult Ovary  (581.8) Adult Testis    99.8 Adult Male Accessory Gland no informative data Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    465.3 Larval Midgut    29.1 Larval Hindgut    39.4 Larval Malpighian Tubules    22.1 Larval Fat Body    52.3 Larval Salivary Gland no informative data Larval Trachea    158.9 Larval Carcass    73.625 Adult Head    13.8 Adult Eye    13.825 Adult Brain    18.1 Adult Thoracic-Abdominal Ganglion    20.3 Adult Crop    17.5 Adult Midgut no informative data Adult Hindgut no informative data Adult Malpighian Tubules    9.6 Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart no informative data Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca no informative data Adult Ovary    581.8 Adult Testis    99.8 Adult Male Accessory Gland no informative data Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    465.3 Larval Midgut    29.1 Larval Hindgut    39.4 Larval Malpighian Tubules    22.1 Larval Fat Body    52.3 Larval Salivary Gland no informative data Larval Trachea    158.9 Larval Carcass    73.625 Adult Head    13.8 Adult Eye    13.825 Adult Brain    18.1 Adult Thoracic-Abdominal Ganglion    20.3 Adult Crop    17.5 Adult Midgut no informative data Adult Hindgut no informative data Adult Malpighian Tubules    9.6 Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart no informative data Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca no informative data Adult Ovary    581.8 Adult Testis    99.8 Adult Male Accessory Gland no informative data Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0003022 expression level Tissue   Expression Level Larval Central Nervous System    465.3 Larval Midgut    29.1 Larval Hindgut    39.4 Larval Malpighian Tubules    22.1 Larval Fat Body    52.3 Larval Salivary Gland no informative data Larval Trachea    158.9 Larval Carcass    73.625 Adult Head    13.8 Adult Eye    13.825 Adult Brain    18.1 Adult Thoracic-Abdominal Ganglion    20.3 Adult Crop    17.5 Adult Midgut no informative data Adult Hindgut no informative data Adult Malpighian Tubules    9.6 Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart no informative data Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca no informative data Adult Ovary    581.8 Adult Testis    99.8 Adult Male Accessory Gland no informative data Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    465.3 Larval Midgut    29.1 Larval Hindgut    39.4 Larval Malpighian Tubules    22.1 Larval Fat Body    52.3 Larval Salivary Gland no informative data Larval Trachea    158.9 Larval Carcass    73.625 Adult Head    13.8 Adult Eye    13.825 Adult Brain    18.1 Adult Thoracic-Abdominal Ganglion    20.3 Adult Crop    17.5 Adult Midgut no informative data Adult Hindgut no informative data Adult Malpighian Tubules    9.6 Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart no informative data Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca no informative data Adult Ovary  581.8 Adult Testis    99.8 Adult Male Accessory Gland no informative data Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    465.3 Larval Midgut    29.1 Larval Hindgut    39.4 Larval Malpighian Tubules    22.1 Larval Fat Body    52.3 Larval Salivary Gland no informative data Larval Trachea    158.9 Larval Carcass    73.625 Adult Head    13.8 Adult Eye    13.825 Adult Brain    18.1 Adult Thoracic-Abdominal Ganglion    20.3 Adult Crop    17.5 Adult Midgut no informative data Adult Hindgut no informative data Adult Malpighian Tubules    9.6 Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart no informative data Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca no informative data Adult Ovary    581.8 Adult Testis    99.8 Adult Male Accessory Gland no informative data Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    465.3 Larval Midgut    29.1 Larval Hindgut    39.4 Larval Malpighian Tubules    22.1 Larval Fat Body    52.3 Larval Salivary Gland no informative data Larval Trachea    158.9 Larval Carcass    73.625 Adult Head    13.8 Adult Eye    13.825 Adult Brain    18.1 Adult Thoracic-Abdominal Ganglion    20.3 Adult Crop    17.5 Adult Midgut no informative data Adult Hindgut no informative data Adult Malpighian Tubules    9.6 Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart no informative data Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca no informative data Adult Ovary    581.8 Adult Testis    99.8 Adult Male Accessory Gland no informative data Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland no informative data Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut no informative data Adult Hindgut no informative data Adult Malpighian Tubules     Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart no informative data Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca no informative data Adult Ovary     Adult Testis     Adult Male Accessory Gland no informative data Adult Carcass no informative data FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    13.8   NA  Eye    13.825   NA  Brain    18.1   465.3  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    20.3   NA  Crop    17.5   29.1  Midgut    no informative data   39.4  Hindgut    no informative data   22.1  Malpighian Tubules    9.6   52.3  Fat Body    no informative data   no informative data  Salivary Gland    no informative data   NA  Heart    no informative data   158.9  Trachea    NA   NA  VirginFemale Spermatheca    no informative data   NA  InseminatedFemale Spermatheca    no informative data   NA  Ovary    581.8   NA  Testis    99.8   NA  Male Accessory Gland    no informative data   73.625  Carcass    no informative data modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_06
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0003022 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG5581-RA FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for Ote Stages(s) 1-3 Stages(s) 4-6 Stages(s) 7-8 Stages(s) 9-10 Stages(s) 11-12 Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable, with Scer\GAL4pnr-MD237 OteGD2067 Sterility Allele female sterile OteEMS Phenotype manifest in Allele female germline stem cell OteEMS female germline stem cell | germline clone OteB279 OteEMS female germline stem cell | supernumerary Otenos.PJ ovary OteEMS
Classical Alleles ( 3 )
For All Classical Alleles Show
Allele of Ote Class Mutagen Stocks Known lesion OteB279 1 Yes OteEMS ethyl methanesulfonate 0 Yes Oteunspecified 0 --
Alleles Carried on Transgenic Constructs ( 11 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Ote Class Mutagen Stocks Known lesion OteGD2067 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes OteKK109866 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Ote+t5.9 in vitro construct - genomic fragment 0 Yes OteGAL4BD.T:Zzzz\FLAG.Ote.ΔLEM in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes Otenos.PJ in vitro construct - regulatory fusion 0 Yes OteScer\UAS.P\T.cJa in vitro construct - regulatory fusion 0 Yes OteT:Avic\GFP in vitro construct - coding region fusion 0 Yes OteT:Zzzz\FLAG in vitro construct - coding region fusion 0 Yes OteΔLEM in vitro construct - deletion 0 Yes OteΔTM.T:Avic\GFP in vitro construct - deletion 0 Yes OteΔTM in vitro construct - deletion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2R)PC4 (Jiang et al., 2008)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD2067} NA P{KK109866} NA P{UASp-Ote.J} NA characterization construct P{nos-Ote.J} NA P{OteP-Flag-GAL4BD-Ote.50-424} NA P{OteP-flag-Ote} NA P{OteP-gfp-Ote.ΔTM} NA P{OteP-gfp-Ote} NA P{OteP-Ote::Emerin.TM} NA P{OteP-Ote::tkv.TM} NA P{OteP-Ote.ΔLEM} NA P{OteP-Ote.ΔTM} NA P{OteP-Ote} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions PBac{5HPw+}OteB279 NA
Gene Ontology: Function, Process & Cellular Component ( 10 unique terms )
Terms Based on Experimental Evidence ( 10 terms )
Molecular Function
CV term Evidence References
protein binding inferred from physical interaction with Lam (Goldberg et al., 1998) transcription corepressor activity inferred from mutant phenotype (Jiang et al., 2008) transcription factor binding inferred from physical interaction with Med (Jiang et al., 2008)
Biological Process
CV term Evidence References
female germ-line stem cell division inferred from mutant phenotype (Jiang et al., 2008) germ-line stem cell maintenance inferred from mutant phenotype (Jiang et al., 2008) negative regulation of transcription, DNA-dependent inferred from mutant phenotype (Jiang et al., 2008) nuclear envelope reassembly inferred from direct assay (Ashery-Padan et al., 1997) positive regulation of BMP signaling pathway inferred from genetic interaction with tkv (Jiang et al., 2008)
Cellular Component
CV term Evidence References
nuclear inner membrane inferred from direct assay (Ashery-Padan et al., 1997) nuclear membrane inferred from direct assay (Jiang et al., 2008)
Terms Based on Predictions or Assertions ( 1 term )
Molecular Function ( 0 terms)
Biological Process ( 0 terms)
Cellular Component
CV term Evidence References
nuclear inner membrane non-traceable author statement (Swiss-Prot Project Members, 1991.2.1)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Ote allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 62771 DroID - A comprehensive database of gene and protein interactions. • FBgn0003022 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 37090
Orthologs
Genome-wide drosophilid orthologs	Dana\GF12772 Dere\GG20980 Dgri\GH20225 Dmoj\GI19394 Dpse\GA18984 Dsec\GM19914 Dsim\GD25400 Dvir\GJ22457 Dwil\GK23240 Dyak\GE13920
Curated drosophilid orthologs	Dpse\GA18984
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AAEL001580-PA Aedes aegypti (mosquito) AGAP007603-PA Anopheles gambiae (mosquito) CPIJ000243 Culex pipens (mosquito) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • FBgn0231562 FBgn0225202 FBgn0212200 FBgn0209568 FBgn0196696 FBgn0174800 FBgn0078983 FBgn0154317 FBgn0142132 FBgn0127689 FBgn0113164 FBgn0089806
Stocks & Reagents
Stocks Listed in FlyBase ( 3 )
Bloomington	16189 w1118; PBac{5HPw+}OteB279/CyO, P{sevRas1.V12}FK1
VDRC	v105308 P{KK109866}VIE-260B v4983 w1118; P{GD2067}v4983
Genomic Clones ( 2 )
	BACR14N05 BACR15G20 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 178 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD41911
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GM04856 GM22065 GM29987 LD10110 LD27345 LD39721 LD42257 LD42546 LD43254 RE05846 RE50544 RE60282 RE70457 RE72466 RE72503 SD23810
Other clones	39842 66410 100365 145600 AT01154 CK01.51A.F9 CK01.58D.F8 CK01.103A.A4 CK00034 Dmel_dig_001550_0590_1464 EK047756 EK063221 EK086634 EK156356 EK223032 EK224806 RP003009195 RP003010506 RP003011217 RP003015398 RP003025913 RP003041358 RP003042759 RP003047866 RP003057944 RP003060201 RP003061981 RP003068181 RP003077789 RP003081171 RP003090622 RP003093804 RP003093966 RP003094257 RP003096259 RP003101986 RP003104943 RP003107184 RP003112086 RP003115523 RP003121325 RP003122039 RP003125533 RP003128085 RP003132897 RP003134806 RP003138562 RP003141011 RP003146592 RP003155984 RP003167112 RP003170814 RP003173555 RP003176370 RP003182690 RP003189384 RP003189586 RP003190592 RP003202697 RP003220016 RP003221693 RP003227938 RP003230159 RP003234440 RP003235007 RP003236325 RP003237885 RP003240798 RP003252622 RP003254788 RP003256959 RP003257736 RP003259339 RP003265392 RP003267408 RP003268679 RP003271214 RP003273134 RP003278198 RP003278540 RP003280750 RP003283088 RP003286320 RP003290831 RP003293120 RP003296256 RP003301984 RP003312260 RP003319621 RP003324722 RP003325961 RP003334322 RP003335992 RP003342762 RP003348397 RP003356113 RP003359011 RP003363578 RP003369300 RP003393664 RP003397781 RP003398196 RP003398445 RP003399679 RP003402348 RP003411358 RP003412514 RP003414944 RP003415621 RP003416282 RP003428023 RP003430227 RP003434583 RP003437334 RP003438970 RP003440095 RP003442384 RP003447771 RP003450921 RP003457219 RP003465787 RP003467762 RP003474361 RP003485628 RP003493322 RP003499645 RP003512734 RP003517079 RP003524547 RP003545610 RP003545967 RP003548005 RP003553015 RP003556710 RP003559629 RP003562149 RP003564566 RP003567784 RP003575362 RP003576143 RP003578492 RP003584284 RP003591333 RP003598097 RP003600824 RP003603725 RP003605797 RP003606869 RP003610898 RP003617004 RP003623519 RP003627302 RP003634623 RP003642732 RP003661321 RP003661847 RP003662100 RP003665292 RP003671591 RP003671598 RP003681305
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0003022 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 37090
Antibody Information
	monoclonal (Harel et al., 1989) polyclonal (Padan et al., 1990, Jiang et al., 2008, Wagner et al., 2004)
Other Information
Discoverer
Etymology
	The name 'otefin' is a transliteration of the Hebrew word meaning 'envelopes'. (Harel et al., 1989)
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments
Other Comments
	Lam protein interacts with both Ote and fs(1)Ya in a yeast two-hybrid system. (Goldberg et al., 1998) Ote behaves as a peripheral protein and is localised to the nucleoplasmic side of the nuclear envelope. Expression studies reveal the C-terminal 11 amino acid hydrophobic sequence is essential for targeting Ote to the nuclear periphery. (Ashery-Padan et al., 1997) Characterisation of Ote suggests an essential role in the assembly of the nuclear envelope. (Ashery-Padan et al., 1997) Ote has been cloned and sequenced. (Padan et al., 1990) Ote protein is not a component of nuclear pore complexes. Some Ote protein is apparently retained in an envelope-like structure throughout mitosis in early Drosophila embryos. (Harel et al., 1989)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_057316 RefSeq (Proteins) • NP_476664 DDBJ / EMBL / GenBank • AAF57722 AAK93364 AC004295 AC004309 AI516149 AW943586 AY051940 CAA35530 X17495 Entrez Gene - A searchable database of RefSeq genes. • 37090 UniProtKB/Swiss-Prot • P20240
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Lamino-associated polypeptide 2/emerin (IPR003887) LEM-like domain (IPR011015)
Linkouts
	BioGRID - A database of protein and genetic interactions • 62771 DroID - A comprehensive database of gene and protein interactions. • FBgn0003022 DRSC - Results from RNAi screens. • FBgn0003022 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0003022 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG5581-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0003022 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 37090 InParanoid A subset of ortholog calls from InParanoid. • AAEL001580-PA Aedes aegypti (mosquito) AGAP007603-PA Anopheles gambiae (mosquito) CPIJ000243 Culex pipens (mosquito) Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /genebrief/otefin.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 37090 modMine - Data generated by the modENCODE project. • FBgn0003022 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • FBgn0231562 FBgn0225202 FBgn0212200 FBgn0209568 FBgn0196696 FBgn0174800 FBgn0078983 FBgn0154317 FBgn0142132 FBgn0127689 FBgn0113164 FBgn0089806
Synonyms & Secondary IDs ( 5 )
Reported As
Symbol Synonym	CG5581 (Keleman et al., 2009.8.5, Haussmann et al., 2008) Ote (Stuart et al., 2007, Herranz et al., 2010, Berman et al., 2004, Doheny et al., 2008, Juhasz et al., 2007, Spresser et al., 2008) ote (Wilmington et al., 2008, Jiang et al., 2008, Sui and Yang, 2011) otefin (Wagner et al., 2004)
Name Synonym	otefin (Cohen et al., 2001, Hita et al., 1999, Goldberg et al., 1998, Ashery-Padan et al., 1997, Padan et al., 1990, Harel et al., 1989, McHugh et al., 2004, Jiang et al., 2008, Pinto et al., 2008, Furukawa et al., 2009, Haussmann et al., 2008, Wagner et al., 2004, Wagner et al., 2006) Otefin (Wilmington et al., 2008, Lancaster et al., 2007, Berman et al., 2004, Somma et al., 2008, Dialynas et al., 2010)
Secondary FlyBase IDs
References ( 46 )
Generate a list of	All references relating to this gene ( 46 )
List References by type	Research paper  ( 29 ) Supplementary material  ( 3 ) Review  ( 1 ) Personal communication to FlyBase  ( 5 ) Abstract  ( 1 ) FlyBase analysis  ( 2 ) Letter  ( 1 ) Protein sequence record  ( 1 ) Computer file  ( 2 ) Automatic genome annotation  ( 1 )
Recent research papers ( 3 )
	Sui and Yang, 2011, J. Genet. Genomics 38(2): 55--61 Distinct effects of nuclear membrane localization on gene transcription silencing in Drosophila S2 cells and germ cells. [FBrf0213132] Dialynas et al., 2010, Development 137(18): 3067--3077 The role of Drosophila Lamin C in muscle function and gene expression. [FBrf0211601] Herranz et al., 2010, Mol. Ecol. 19(19): 4255--4264 Spaceflight-related suboptimal conditions can accentuate the altered gravity response of Drosophila transcriptome. [FBrf0216346] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...