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General Information
Symbol
Dmel\ovo
Species
D. melanogaster
Name
ovo
Annotation Symbol
CG6824
Feature Type
FlyBase ID
FBgn0003028
Gene Model Status
Stock Availability
Gene Snapshot
ovo (ovo) encodes a Zn finger transcription factor, with several isoforms acting as repressor or activator. It is involved in epidermis differentiation, under the control of Wnt and EGFR signaling pathways and Hox factors, as well as female germline differentiation and sex determination. [Date last reviewed: 2019-03-14]
Also Known As
svb, shavenbaby, shaven baby, ovoD, ovo/svb
Key Links
Genomic Location
Cytogenetic map
Sequence location
X:5,048,273..5,070,929 [+]
Recombination map
1-10
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Protein Family (UniProt)
-
Summaries
Gene Group (FlyBase)
C2H2 ZINC FINGER TRANSCRIPTION FACTORS -
Zinc finger C2H2 transcription factors are sequence-specific DNA binding proteins that regulate transcription. They possess DNA-binding domains that are formed from repeated Cys2His2 zinc finger motifs. (Adapted from PMID:1835093, FBrf0220103 and FBrf0155739).
Protein Function (UniProtKB)
Transcriptional regulator with essential functions in the germline and soma (PubMed:9012532, PubMed:12915226, PubMed:10421370). Plays an essential role in regulating the formation of apical cell extensions such as denticles and aristae, and initiating cytoskeletal remodeling during epidermal differentiation (PubMed:12915226, PubMed:10421370). Isoform B: Transcriptional repressor which functions in postembryonic development (PubMed:21527259, PubMed:26383956). The full-length unprocessed form acts as a transcriptional repressor (Transcriptional repressor svb) (PubMed:26383956). Transcriptional activator shavenbaby: Transcriptional activator which initiates trichome development and also promotes tarsal joint development (PubMed:21527259, PubMed:20647469, PubMed:26383956). Has an essential somatic role regulating the tal-dependent formation of trichomes, and initiating cytoskeletal remodeling during epidermal differentiation (PubMed:20647469, PubMed:21527259, PubMed:25344753, PubMed:26383956). Function with SoxN is required for correct denticle morphogenesis on the embryonic epidermis (PubMed:28506986). SoxN and svb appear to act both independently and in conjunction with each other to activate certain genes involved in denticle morphogenesis; Svb appears to be involved in regulating denticle length whereas SoxN regulates the denticle base circumference (PubMed:28506986). Also functions in the development of other apical cell extensions such as bristles (PubMed:25344753). Also has an important role in tarsal joint development, repressing expression of the N ligand Dl and defining its signaling boundary (PubMed:21527259). Isoform C: Transcriptional repressor which is specifically involved in female germline development, where it functions antagonistically to isoform D (PubMed:10648246, PubMed:28059165). Negatively regulates expression of otu and may also have autoregulatory activity (PubMed:10648246). Negatively regulates expression of piwi in the primordial germ cells (PGCs) (PubMed:28059165). Isoform D: Transcriptional activator which is specifically involved in female germline development, where it functions antagonistically to isoform C (PubMed:10648246, PubMed:1712294, PubMed:3428601, PubMed:8652413). Necessary and sufficient for normal oogenesis (PubMed:1712294, PubMed:12051822). Required in the primordial germ cells (PGCs) for normal development of male and female germline cells (PubMed:28059165). Plays a role in germline sex determination (PubMed:2116356). Binds the promoter DNA and positively regulates the transcription of the otu gene in a stage-specific manner (PubMed:10525184, PubMed:11290304, PubMed:9634487). May have autoregulatory activity (PubMed:11290304, PubMed:9634487).
(UniProt, P51521)
Phenotypic Description (Red Book; Lindsley and Zimm 1992)
ovo: ovo (B. Oliver)
All ovo alleles fully penetrant for female sterility; no male function for ovo+ known. Lack of zygotic activity results in complete absence of germ-line cells in adult female; reduction in numbers of germ cells first evident during early gastrulation. Homozygotes for weaker alleles produce germ cells, but oogenesis defective; egg chambers may degenerate prior to vitellogenesis or proceed through oogenesis and be oviposited, depending on allele; laid eggs are permeable to neutral red and never develop. Heterozygotes for two of the dominant alleles phenotypically similar to homozygotes for weak recessive alleles. Ovarian tumors formed in females carrying ovoD3 in heterozygous combination with the hypomorphic alleles, e.g., ovoD1rv20, or with fs(1)A1621. The ovoD2 mutation is partially suppressed by many Sxl alleles. ovoD1/+ females produce no eggs; extensively utilized in the selection of ovo+ germ-line clones.
svb: shaven baby
Homozygous lethal. Many fewer denticles than wild type; remaining denticles small and with characteristic morphology. Denticles arranged in belts in the abdominal segments; absent from thoracic segments. Cell autonomous in mosaics; useful in studying embryonic mosaics.
Summary (Interactive Fly)
zinc finger transcription factor - involved in ovarian maturation, sex determination, segment polarity
Gene Model and Products
Number of Transcripts
5
Number of Unique Polypeptides
4

Please see the GBrowse view of Dmel\ovo or the JBrowse view of Dmel\ovo for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model
Gene model reviewed during 5.43
Low-frequency RNA-Seq exon junction(s) not annotated.
Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.
Gene model reviewed during 5.52
Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0070740
5946
975
FBtr0070738
6606
1351
FBtr0070739
5518
1222
FBtr0100408
5948
1028
FBtr0301914
6763
1351
Additional Transcript Data and Comments
Reported size (kB)
5.0 (northern blot)
5.0, 4.8 (northern blot)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0070708
104.8
975
7.31
FBpp0070706
146.8
1351
7.04
FBpp0070707
132.4
1222
6.99
FBpp0099822
110.7
1028
7.36
FBpp0291128
146.8
1351
7.04
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

1351 aa isoforms: ovo-PB, ovo-PE
Additional Polypeptide Data and Comments
Reported size (kDa)
1028, 850 (aa); 110.6, 92 (kD predicted)
131 (kD predicted)
Comments
The ovo protein is at least 1209 amino acids long. In addition to four zinc finger repeats, the protein contains homopolymeric runs of several different amino acids, and has an extremely hydrophilic amino acid profile.
External Data
Subunit Structure (UniProtKB)
Isoform B: Interacts (via N-terminus) with Ubr3; the interaction is mediated by tal.
(UniProt, P51521)
Post Translational Modification
Isoform B: N-terminus is proteolytically cleaved and ubiquitinated via a tal-dependent mechanism, leading to the proteolytic degradation of the N-terminus and the production of transcriptional activator shavenbaby, a truncated form with transcriptional activator activity.
(UniProt, P51521)
Domain
Only 3 of the 4 C2H2-type zinc-fingers are required for DNA-binding. Based on its marked lack of evolutionary conservation, the fourth zinc-finger is unlikely to be required.
(UniProt, P51521)
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\ovo using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Gene Ontology (21 terms)
Molecular Function (4 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
inferred from direct assay
(assigned by UniProt )
Terms Based on Predictions or Assertions (4 terms)
CV Term
Evidence
References
traceable author statement
inferred from biological aspect of ancestor with PANTHER:PTN000798648
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000798648
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000798648
(assigned by GO_Central )
Biological Process (16 terms)
Terms Based on Experimental Evidence (14 terms)
CV Term
Evidence
References
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
(assigned by UniProt )
inferred from mutant phenotype
(assigned by UniProt )
inferred from mutant phenotype
inferred from mutant phenotype
(assigned by UniProt )
inferred from genetic interaction with UniProtKB:P19339
(assigned by UniProt )
inferred from mutant phenotype
inferred from mutant phenotype
(assigned by UniProt )
inferred from mutant phenotype
(assigned by UniProt )
inferred from mutant phenotype
inferred from mutant phenotype
(assigned by UniProt )
inferred from mutant phenotype
inferred from mutant phenotype
(assigned by UniProt )
inferred from mutant phenotype
(assigned by UniProt )
Terms Based on Predictions or Assertions (5 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000798649
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000798648
(assigned by GO_Central )
traceable author statement
Cellular Component (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
inferred from direct assay
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
inferred by curator from GO:0003677
(assigned by UniProt )
inferred from biological aspect of ancestor with PANTHER:PTN000798648
(assigned by GO_Central )
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
stomatogastric nervous system

Comment: late embryonic stage 13

dorsal ridge

Comment: late embryonic stage 13

dorsal hair

Comment: late embryonic stage 13

denticle belt | segmentally repeated

Comment: late embryonic stage 13

denticle field

Comment: more pronounced in stout bristle rows than in fine hair rows

organism

Comment: maternally deposited

northern blot
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
At embryonic stage 9, ovo is expressed in the six rows of cells in each segment that will go on to form the six rows of denticles.
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\ovo in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 1-3
  • Stages(s) 4-6
  • Stages(s) 7-8
  • Stages(s) 11-12
  • Stages(s) 13-16
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 146 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 76 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of ovo
Transgenic constructs containing regulatory region of ovo
Deletions and Duplications ( 41 )
Partially disrupted in
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Sterility
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
adult abdomen & trichome | somatic clone
adult thorax & microchaeta, with Scer\GAL4Bx-MS1096
adult thorax & microchaeta | somatic clone
adult thorax & trichome, with Scer\GAL4Bx-MS1096
adult thorax & trichome | somatic clone
filamentous actin & embryonic/first instar larval cuticle | ectopic, with Scer\GAL4ptc-559.1
filamentous actin & embryonic/first instar larval cuticle | ectopic | somatic clone, with Scer\GAL4Act5C.PI
Orthologs
Human Orthologs (via DIOPT v7.1)
Homo sapiens (Human) (3)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
9 of 15
Yes
Yes
8 of 15
No
Yes
2 of 15
No
Yes
Model Organism Orthologs (via DIOPT v7.1)
Mus musculus (laboratory mouse) (3)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
8 of 15
Yes
Yes
7 of 15
No
Yes
1 of 15
No
Yes
Rattus norvegicus (Norway rat) (4)
7 of 13
Yes
Yes
5 of 13
No
Yes
2 of 13
No
Yes
2 of 13
No
Yes
Xenopus tropicalis (Western clawed frog) (3)
5 of 12
Yes
Yes
3 of 12
No
Yes
1 of 12
No
Yes
Danio rerio (Zebrafish) (7)
6 of 15
Yes
Yes
4 of 15
No
Yes
4 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (1)
9 of 15
Yes
Yes
Arabidopsis thaliana (thale-cress) (0)
No records found.
Saccharomyces cerevisiae (Brewer's yeast) (1)
1 of 15
Yes
Yes
Schizosaccharomyces pombe (Fission yeast) (0)
No records found.
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG091907B7 )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila melanogaster
fruit fly
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila sechellia
Drosophila erecta
Drosophila yakuba
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila persimilis
Drosophila willistoni
Drosophila virilis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091502AN )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Glossina morsitans
Tsetse fly
Glossina morsitans
Tsetse fly
Mayetiola destructor
Hessian fly
Aedes aegypti
Yellow fever mosquito
Anopheles darlingi
American malaria mosquito
Anopheles gambiae
Malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W023I )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Danaus plexippus
Monarch butterfly
Heliconius melpomene
Postman butterfly
Apis florea
Little honeybee
Apis mellifera
Western honey bee
Bombus impatiens
Common eastern bumble bee
Bombus terrestris
Buff-tailed bumblebee
Linepithema humile
Argentine ant
Megachile rotundata
Alfalfa leafcutting bee
Nasonia vitripennis
Parasitic wasp
Dendroctonus ponderosae
Mountain pine beetle
Dendroctonus ponderosae
Mountain pine beetle
Tribolium castaneum
Red flour beetle
Pediculus humanus
Human body louse
Rhodnius prolixus
Kissing bug
Cimex lectularius
Bed bug
Acyrthosiphon pisum
Pea aphid
Zootermopsis nevadensis
Nevada dampwood termite
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X020Y )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Ixodes scapularis
Black-legged tick
Stegodyphus mimosarum
African social velvet spider
Tetranychus urticae
Two-spotted spider mite
Tetranychus urticae
Two-spotted spider mite
Tetranychus urticae
Two-spotted spider mite
Tetranychus urticae
Two-spotted spider mite
Tetranychus urticae
Two-spotted spider mite
Tetranychus urticae
Two-spotted spider mite
Tetranychus urticae
Two-spotted spider mite
Tetranychus urticae
Two-spotted spider mite
Tetranychus urticae
Two-spotted spider mite
Tetranychus urticae
Two-spotted spider mite
Tetranychus urticae
Two-spotted spider mite
Tetranychus urticae
Two-spotted spider mite
Tetranychus urticae
Two-spotted spider mite
Tetranychus urticae
Two-spotted spider mite
Daphnia pulex
Water flea
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0LB1 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Ciona intestinalis
Vase tunicate
Gallus gallus
Domestic chicken
Paralogs
Paralogs (via DIOPT v7.1)
Drosophila melanogaster (Fruit fly) (1)
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 1 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    esyN Network Diagram
    Show neighbor-neighbor interactions:
    Select Layout:
    Legend:
    Protein
    RNA
    Selected Interactor(s)
    Interactions Browser

    Please see the Physical Interaction reports below for full details
    protein-protein
    Physical Interaction
    Assay
    References
    Summary of Genetic Interactions
    esyN Network Diagram
    esyN Network Key:
    Suppression
    Enhancement

    Please look at the allele data for full details of the genetic interactions
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    External Data
    Subunit Structure (UniProtKB)
    Isoform B: Interacts (via N-terminus) with Ubr3; the interaction is mediated by tal.
    (UniProt, P51521 )
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DroID - A comprehensive database of gene and protein interactions.
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    MIST (genetic) - An integrated Molecular Interaction Database
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Pathways
    Signaling Pathways (FlyBase)
    Metabolic Pathways
    External Data
    Linkouts
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    X
    Recombination map
    1-10
    Cytogenetic map
    Sequence location
    X:5,048,273..5,070,929 [+]
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    4E2-4E2
    Limits computationally determined from genome sequence between P{EP}EP425 and P{EP}CG3249EP1400
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    4E1-4E2
    (determined by in situ hybridisation)
    Experimentally Determined Recombination Data
    Location
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (45)
    Genomic Clones (16)
     

    Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

    cDNA Clones (76)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequences
    BDGP DGC clones
    Other clones
    Drosophila Genomics Resource Center cDNA clones

    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

    cDNA Clones, End Sequenced (ESTs)
    RNAi and Array Information
    Linkouts
    DRSC - Results frm RNAi screens
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    Antibody Information
    Laboratory Generated Antibodies
     
    polyclonal
    Commercially Available Antibodies
     
    Other Information
    Relationship to Other Genes
    Source for database identify of
    Source for identity of: ovo CG6824
    Source for database merge of
    Source for merge of: ovo CG15467
    Additional comments
    Annotations CG6824 and CG15467 merged as CG6824 (which corresponds to ovo) in release 3 of the genome annotation.
    Recessive alleles, mostly recovered as revertants of dominant alleles, usually but not always, simultaneously mutant lzl; The lzl eye phenotype is cold-sensitive and semidominant. Most revertants are amorphic in that, when tested in heterozygous combination with ovoD1rv22 or ovoD1rv23, they have atrophic ovaries, with no egg chambers observable.
    Other Comments
    Expression is enriched in embryonic gonads.
    ovo is necessary and sufficient for cells to grow F-actin based extensions.
    The ovo-A and ovo-B isoforms have opposite regulatory activities.
    ovo-B function is required during oogenesis, while ovo-A is important for subsequent embryonic germline development.
    ovo is required for the differentiation of XX germ cells during larval gonial stages. ovo may also have a role in other oogenic functions.
    Transcriptional regulation of ovo integrates inputs from the wg and Egfr pathways and control epidermis differentiation.
    ovo is a key selector gene that, cell autonomously, directs cytoskeletal modifications producing the denticle.
    Analysis of dominant-negative ovoD mutations suggests that the dominant activity is due to the new initiation codons in the ovo-B isoform mRNAs and not due to the amino acid substitutions in the ovo-A isoform.
    ovo protein regulates its own and the otu promoters.
    Absence of ovo+ in females and extra copies of ovo+ in males do not result in reciprocal phenotypes.
    High ovo+ copy number is not sufficient for overt development of XY germ cells. The absence of ovo+ in XX germ cells results in altered sex determination and cell viability, but increased ovo+ activity in XY germ cells is insufficient to impair germline viability and is probably insufficient to alter sexual identity.
    A method that allows the efficient recovery of female germline mosaics for mutations localised n the autosomes has been developed using P{ovoD1-18} P{FRT(whs)} P{hsFLP}.
    The dominant-negative effects of the ovoD mutations are likely to arise from the expression of novel protein isoforms, starting during germline cell differentiation.
    Female germ cells require zygotic ovo function for survival mainly during the second larval, third larval and pupal stages.
    Mapping of gypsy insertions into the ovo gene reveals a global insertion specificity rather than a specificity for a local nucleotide consensus sequence.
    Nurse cell-specific genes are functional in the pseudonurse cells of otu mutants, but the transport of pum, otu, ovo and bcd RNAs to the cytoplasm is affected.
    The structure and expression of the complex ovo locus is characterised; differential embryonic expression of ovo and svb transcripts suggests the existance of several isoform ovo protein products.
    otu and ovo are required cell autonomously in the female germline for germ cell proliferation and differentiation. XY germ cells do not require otu when developing in testes, but become dependent on otu function for proliferation when placed in an ovary. The requirement for ovo is dependent on a cell autonomous signal derived from the X:A ratio. The differential regulation of the otu and ovo genes provides a mechanism for the female germline to incorporate both somatic and cell-autonomous inputs required for oogenesis.
    300 deficiency chromosomes have been screened for enhancers and suppressors of the ovo mutant phenotype. More than a dozen small regions probably contain genes whose products act in developmental hierarchies that include ovo+ protein.
    Zygotic ovo activity is not essential for pole cell viability or proliferation during stages of embryogenesis. Pupal gonads fail to produce egg chambers indicating that the agametic adult phenotype is caused by a block in oogenesis before cyst formation, rather than the degeneration of existing egg chambers. ovo function is not essential for germline viability before pupariation.
    ovo, otu, snf and Sxl are not involved in the early events of germline sex determination, but are required later, during metamorphosis or in the adult for oogenesis.
    Alternative splicing generates two types of transcripts from the ovo gene.
    An insertion of a transgene containing the ovoD1 mutation onto an autosome can confer a dominant female sterile phenotype.
    High level of ovo+ expression depends on the number of X chromosomes, not the somatic sexual signals. The ovo+ gene products are required for events occurring only in flies with two X chromosomes.
    The genetic hierarchy regulating female germ-line sex determination includes tra, tra2, dsx, fu, otu, ovo, snf and Sxl. otu+, ovo+ and snf+ activities are required for female-specific Sxl+ pre-mRNA splicing within 2X germ-line cells.
    Gene dosage studies demonstrate additional interaction of with mutations at otu with the ovo locus.
    Phenotypic analysis of mutations suggests that ovo encodes a function necessary for normal development of the oocyte.
    ovo appears to be involved in germ line sex determination and is linked in some manner to sex determination in the soma.
    Molecular analysis of ovo revertants from a cross with the y v f mal strain demonstrate they have acquired either a gypsy or copia insertion at cytological location 4E.
    Homozygous females and females of the genotype Df(1)HC244, SxlM1/ovo are sterile with ovaries devoid of germ cells: ovo must act downstream of Sxl or in a different pathway.
    ovo is required for early female germ line maintenance but is not required for male germ line stability.
    ovo mutants display short denticles that are reduced in number and an absence of dorsal hairs.
    The expression of genes controlling neurogenesis is dependent on the previous activity of the genes controlling the development of the embryonic dorsal-ventral pattern.
    All ovo alleles fully penetrant for female sterility; no male function for ovo+ known. Lack of zygotic activity results in complete absence of germ-line cells in adult female; reduction in numbers of germ cells first evident during early gastrulation. Homozygotes for weaker alleles produce germ cells, but oogenesis defective; egg chambers may degenerate prior to vitellogenesis or proceed through oogenesis and be oviposited, depending on allele; laid eggs are permeable to neutral red and never develop. Heterozygotes for two of the dominant alleles phenotypically similar to homozygotes for weak recessive alleles. Ovarian tumors formed in females carrying ovoD3 in heterozygous combination with the hypomorphic alleles, e.g., ovoD1rv20, or with snf. The ovoD2 mutation is partially suppressed by many Sxl alleles. ovoD1/+ females produce no eggs; extensively utilized in the selection of ovo+ germ-line clones. svb alleles are homozygous lethal. Many fewer denticles than wild type; remaining denticles small and with characteristic morphology. Denticles arranged in belts in the abdominal segments; absent from thoracic segments. Cell autonomous in mosaics; useful in studying embryonic mosaics.
    Origin and Etymology
    Discoverer
    Etymology
    Identification
    External Crossreferences and Linkouts ( 74 )
    Sequence Crossreferences
    NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
    GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
    GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
    RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
    UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
    Other crossreferences
    BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
    Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
    Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    Flygut - An atlas of the Drosophila adult midgut
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
    InterPro - A database of protein families, domains and functional sites
    KEGG Genes - Molecular building blocks of life in the genomic space.
    modMine - A data warehouse for the modENCODE project
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DPiM - Drosophila Protein interaction map
    DroID - A comprehensive database of gene and protein interactions.
    DRSC - Results frm RNAi screens
    FLIGHT - Cell culture data for RNAi and other high-throughput technologies
    FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
    FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
    FlyMine - An integrated database for Drosophila genomics
    Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    MIST (genetic) - An integrated Molecular Interaction Database
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Synonyms and Secondary IDs (24)
    Reported As
    Symbol Synonym
    Fs(1)K1237
    Fs(1)K155
    fs(1)M1
    fs(1)M38
    ovo/shavenbaby
    Secondary FlyBase IDs
    • FBgn0000825
    • FBgn0000849
    • FBgn0003650
    • FBgn0029741
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    References (362)