• Home
• Tools
  • Tools Overview
  • Query Tools

    
    • QuickSearch
    • QueryBuilder
    • TermLink
    • CytoSearch
    • Interactions Browser
    • ImageBrowse
    • Find A Person
    • Google™ FlyBase
  • Genomic/Map Tools

    
    • BLAST
    • GBrowse
    • CytoSearch
    • Aberrations Maps
    • Chromosome Maps
    • Coordinates Converter
  • Retrieve/Convert

    
    • Batch Download
    • Coordinates Converter
    • ID Converter
  • Data Submission

    
    • Fast-Track Your Paper
    • Submit Personal Comm
    • Add A New Person
    • Update Person Info
  • People

    
    • Find A Person
    • Add A New Person
    • Update Person Info
• Files
  • Files Overview
  • Downloads:
  • Precomputed files
  • Archived data
  • Releases (FTP)
  • Genomes (FTP)
  • AAA data (FTP)
  • Lists:
  • Transposons (Dmel)
  • Transposons (other)
  • Vectors & Constructs
  • Anatomical Terms
  • Map Conversion
• Species
  • Phylogeny
  • Sequenced Species
  • Synteny Table
  • Color Illustrations
  • Abbreviations
• Documents
  • About FlyBase
  • Reference Manual:
  • Sections
  • Contents
  • Nomenclature
  • Release Notes
• Resources
  • All Resources
  • Model Organisms
  • Stock Collections
  • Other links:
  • BDGP
  • DGRC
  • FlyExpress
  • Interactive Fly
  • modENCODE
  • Textpresso for Fly
• News
  • News
  • FlyBase Forum
  • FlyBase Positions
  • Meetings
  • Courses
  • Fly Board
  • White papers
  • Funding
  • bionet.dros
• Help
  • Google™ FlyBase
  • Site Map
  • FAQs
  • FlyBase Guides
  • Video Tutorials
  • Report help
  • New to Flies
  • Pers. comm.
  • Author guidelines
  • Contact FlyBase
• Archives
  • Prior Release
  • Other Archives
  • Coordinate Converter

Gene Dmel\Pcl

General Information
Symbol	Dmel\Pcl	Species	D. melanogaster
Name	Polycomblike	Annotation symbol	CG5109
Feature type	protein_coding_gene	FlyBase ID	FBgn0003044
Gene Model Status	Current	Stock availability	16 publicly available
Also Known As	l(2)s1859
Genomic Location
Chromosome (arm)	2R	Recombination map	2-84
Cytogenetic map	55B8-55B8	Sequence location	2R:14,022,934..14,026,947 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Polycomblike is referred to in FlyBase by the symbol Dmel\Pcl (CG5109, FBgn0003044). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: sequence-specific DNA binding transcription factor activity; protein binding; chromatin binding. There is experimental evidence that it is involved in the biological process: negative regulation of transcription, DNA-dependent; neurogenesis. 40 alleles are reported. The phenotypes of these alleles are annotated with: adult segment; embryonic abdomen; organ system subdivision; embryonic segment; larval abdominal segment; organ system; abdominal segment 7; adult mesothoracic segment; gland; larval abdominal segment 5; midgut constriction; embryonic abdominal segment 4; antennal segment. It has one annotated transcript and one annotated polypeptide. Protein features are: Tudor domain; Zinc finger, FYVE/PHD-type; Zinc finger, PHD-finger; Zinc finger, PHD-type; Zinc finger, PHD-type, conserved site; Zinc finger, RING/FYVE/PHD-type. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of low expression. Peak expression observed within 00-06 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval/adult central nervous system, larval salivary gland, adult ovary. Comments on Affy2 ProbeSet: ProbeSet 1623525_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of Pcl. Gene sequence location is 2R:14022934..14026947.
External Summaries
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	Pcl: Polycomblike Pcl+, like Pc+, may be considered a negative regulator of the BXC and the ANTC. Strong Pcl mutant alleles (Pcl1, Pcl2) are homozygous lethal, hemizygous lethal, and lethal with each other, the embryos having partial posteriorly-directed transformations of the abdominal segments (segment 1 into segment 2 and segments 2-7 into more posterior segments). These mutants are strong enhancers of Pc3, showing Pc-like transformations in embryos, and are indistinguishable from Pcl deficiencies in complementation behavior. Extreme posteriorly-directed segmental transformations are found in Pcl1/Df(2R)Pcl11B;Pc3/Pc3 lethal embryos (Duncan, 1982); these embryos are considerably more abnormal than the Pc3 homozygotes that are Pcl+. Pcl/Pcl embryos that are also homozygous for Asx, Psc, or Scm show strong posteriorly-directed transformations of the entire body pattern; the triple mutant Psc Asx Pcl has a tandem array of posterior abdominal segments including four abdominal denticle bands in the head region (Jurgens, 1985). Pcl1/Pcl1 clones in the second through the sixth abdominal tergites also show caudal transformations; similar clones in the wing often appear to be partially transformed into haltere tissue. Weak Pcl alleles (Pcl3, Pcl4) survive, at least through eclosion, either as homozygotes or as heterozygotes with strong or weak Pcl alleles (Duncan, 1982). Pcl1/Pcl3 and Pcl1/Pcl4 show segmental transformations characteristic of Pc/+ flies, i.e. antenna to leg, second and third leg to first leg, wing to haltere, and posteriorly-directed abdominal transformations (the most frequent abnormality). Increasing the dosage of the BXC in Pcl1/Pcl4 heterozygotes suppresses the posteriorly-directed transformations, but enhances the transformations from leg2 and leg3 to leg1; increasing the dosage of the ANTC enhances both types of transformations (Duncan, 1982). Pcl1/Pcl3 females, which die before or soon after eclosion, show a conversion of parovaria to spermathecae. Both strong and weak Pcl alleles are viable as heterozygotes (Pcl/+) and may show partial abdominal and leg transformations, the abnormalities increasing in Pcl/+;Pc/+ flies or when Pcl/+ is combined with E(Pc).
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2011_10	References Park et al., 2011, PLoS Genet. 7(8): e1002241 Neumüller et al., 2011, Cell Stem Cell 8(5): 580--593
FB2012_01	References Gibert et al., 2011, PLoS Genet. 7(1): e1001280 Sequence features HFA07503 BKN20301 BKN45657 Controlled Vocabulary Terms chromatin binding
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 55B8-55B8   Limits computationally determined from genome sequence between P{PZ}Hsf03091&P{EP}Dgp-1EP731 and P{PZ}sbb04525&P{EP}EP1081EP1081
Experimentally Determined Cytological Location
Cytogenetic map Notes References 55A-55A     (Lonie, 1995.3.28) 55B5-55B6   (determined by in situ hybridisation)   (Spradling et al., 1999) 55B4-55B6   55B5--6   (BDGP Project Members, 1994-1999) 55B-55C   Location from complementation analysis with deficiency and duplication chromosomes (details unspecified).   (Kennison and Tamkun, 1988)
Experimentally Determined Recombination Data
Location	2-84 (Kennison and Tamkun, 1988, ) 2-83 (Jurgens, 1985) 2-86 (Tearle and Nusslein-Volhard, 1987)
Left of (cM)
Right of (cM)	c (Doane, 1999)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Pcl for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Pcl-RA FBtr0086735  NM_057324   3833   1043
Additional Transcript Data & Comments
Reported size (kB)	3.9 (northern blot) (Lonie et al., 1994)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Pcl-PA   FBpp0085914   114.6   1043   9.08     NP_476672   AAF57748
Additional Polypeptide Data & Comments
Reported size (kDa)	857 (aa) (Lonie et al., 1994)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Zinc finger, PHD-type (IPR001965) Tudor domain (IPR002999) Zinc finger, FYVE/PHD-type (IPR011011) Zinc finger, PHD-type, conserved site (IPR019786) Zinc finger, PHD-finger (IPR019787) Zinc finger, RING/FYVE/PHD-type (IPR013083)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA567667   AA696105   AA801495   AA820160   AA941479   AA949359   AE013599 AAF57748   AC004296   AI063158   AI403521   AI518606   AI531271   AI542177   AI542964   AQ026142   AW944600   AY075585 AAL68389   BF486487   BF488137   BF489388   BF492152   BF495464   BF500107   BF500445   BF501029   BF502378   BG634463   BG638864   BI164067   BI166934   BI242372   BI369137   BI638243   CK657715   CL705829   CO179696   CO189312   CO275615   CO277761   CO291565   CO311937   CO312183   CO313384   CO319998   CO325221   CO335303   CO343951   CZ471085   CZ471264   EC059029   EC061026   EC068152   EC071442   EC075534   EC205880   EC212339   EC213972   EC225420   EC225421   EC229058   EC229270   EC230399   EC230962   EC240312   EC240338   EC246692   EC246848   EC247982   EC255036   GH750458   GH750905   GH772364   GH772431   GH772603   GH785093   GH793855   GH797848   GH820003   GH820559   GH822632   GH823808   GH824443   GH827677   GH828205   GH828932   GH829369   GH832161   GH833828   GH833957   GH836063   GH858170   GH859997   GH863745   GH864383   GH865192   GH865401   GH868506   GH868686   GH870164   GH874501   GH880380   GH882813   GH885019   GH886785   GH897656   GH903693   GH907177   GH908395   GH908530   GH911346   GH913534   GH916682   GH918597   GH919273   GH920078   GH924424   GH926342   GH933831   GH935504   GH938330   GH939707   GH939925   GH943979   L35153 AAA64457   Z31895   Z32189
UniProtKB/Swiss-Prot	Q24459
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage   organism | ubiquitous   (Lonie et al., 1994)
Additional Descriptive Data
	Pcl transcripts are distributed evenly throughout all tissues at all stages of embryonic development. (Lonie et al., 1994)
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
immunolocalization Stage Tissue/Position (including subcellular localization) Reference larval stage   embryonic/larval salivary gland   • polytene chromosome (Lonie et al., 1994)
Additional Descriptive Data
	Pcl protein binds to approximately 100 sites on the polytene chromosomes. It binds to the identical set of sites bound by Pc and phl proteins. (Lonie et al., 1994)
Marker for
Subcellular Localization
CV Term	nucleus (Lonie et al., 1994)
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0003044    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0003044 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of low expression. Peak expression observed within 00-06 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0003044 expression level Developmental Stage   Expression Level embryo 00-02hr    6045 embryo 02-04hr    2669 embryo 04-06hr    2510 embryo 06-08hr    2217 embryo 08-10hr    1607 embryo 10-12hr    998 embryo 12-14hr    952 embryo 14-16hr    879 embryo 16-18hr    527 embryo 18-20hr    339 embryo 20-22hr    247 embryo 22-24hr    271 larva L1    607 larva L2    465 larva L3 12hr old    370 larva L3 puffstage 1-2    385 larva L3 puffstage 3-6    856 larva L3 puffstage 7-9    888 white prepupae new    990 white prepupae 12hr    1219 white prepupae 24hr    1406 pupae 2d postWPP    960 pupae 3d postWPP    455 pupae 4d postWPP    579 adult male 01day    506 adult male 05day    705 adult male 30day    729 adult female 01day    2109 adult female 05day    3689 adult female 30day    3811 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (6045) embryo 02-04hr  (2669) embryo 04-06hr  (2510) embryo 06-08hr  (2217) embryo 08-10hr  (1607) embryo 10-12hr  (998) embryo 12-14hr  (952) embryo 14-16hr  (879) embryo 16-18hr  (527) embryo 18-20hr  (339) embryo 20-22hr  (247) embryo 22-24hr  (271) larva L1  (607) larva L2  (465) larva L3 12hr old  (370) larva L3 puffstage 1-2  (385) larva L3 puffstage 3-6  (856) larva L3 puffstage 7-9  (888) white prepupae new  (990) white prepupae 12hr  (1219) white prepupae 24hr  (1406) pupae 2d postWPP  (960) pupae 3d postWPP  (455) pupae 4d postWPP  (579) adult male 01day  (506) adult male 05day  (705) adult male 30day  (729) adult female 01day  (2109) adult female 05day  (3689) adult female 30day  (3811) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (6045) embryo 02-04hr  (2669) embryo 04-06hr  (2510) embryo 06-08hr  (2217) embryo 08-10hr  (1607) embryo 10-12hr    998 embryo 12-14hr    952 embryo 14-16hr    879 embryo 16-18hr    527 embryo 18-20hr    339 embryo 20-22hr    247 embryo 22-24hr    271 larva L1    607 larva L2    465 larva L3 12hr old    370 larva L3 puffstage 1-2    385 larva L3 puffstage 3-6    856 larva L3 puffstage 7-9    888 white prepupae new    990 white prepupae 12hr    1219 white prepupae 24hr  1406 pupae 2d postWPP    960 pupae 3d postWPP    455 pupae 4d postWPP    579 adult male 01day    506 adult male 05day    705 adult male 30day    729 adult female 01day  (2109) adult female 05day  (3689) adult female 30day  (3811) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    6045 embryo 02-04hr    2669 embryo 04-06hr    2510 embryo 06-08hr    2217 embryo 08-10hr    1607 embryo 10-12hr    998 embryo 12-14hr    952 embryo 14-16hr    879 embryo 16-18hr    527 embryo 18-20hr    339 embryo 20-22hr    247 embryo 22-24hr    271 larva L1    607 larva L2    465 larva L3 12hr old    370 larva L3 puffstage 1-2    385 larva L3 puffstage 3-6    856 larva L3 puffstage 7-9    888 white prepupae new    990 white prepupae 12hr    1219 white prepupae 24hr    1406 pupae 2d postWPP    960 pupae 3d postWPP    455 pupae 4d postWPP    579 adult male 01day    506 adult male 05day    705 adult male 30day    729 adult female 01day    2109 adult female 05day    3689 adult female 30day    3811 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    6045 embryo 02-04hr    2669 embryo 04-06hr    2510 embryo 06-08hr    2217 embryo 08-10hr    1607 embryo 10-12hr    998 embryo 12-14hr    952 embryo 14-16hr    879 embryo 16-18hr    527 embryo 18-20hr    339 embryo 20-22hr    247 embryo 22-24hr    271 larva L1    607 larva L2    465 larva L3 12hr old    370 larva L3 puffstage 1-2    385 larva L3 puffstage 3-6    856 larva L3 puffstage 7-9    888 white prepupae new    990 white prepupae 12hr    1219 white prepupae 24hr    1406 pupae 2d postWPP    960 pupae 3d postWPP    455 pupae 4d postWPP    579 adult male 01day    506 adult male 05day    705 adult male 30day    729 adult female 01day    2109 adult female 05day    3689 adult female 30day    3811 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0003044 expression level Developmental Stage   Expression Level embryo 00-02hr    6045 embryo 02-04hr    2669 embryo 04-06hr    2510 embryo 06-08hr    2217 embryo 08-10hr    1607 embryo 10-12hr    998 embryo 12-14hr    952 embryo 14-16hr    879 embryo 16-18hr    527 embryo 18-20hr    339 embryo 20-22hr    247 embryo 22-24hr    271 larva L1    607 larva L2    465 larva L3 12hr old    370 larva L3 puffstage 1-2    385 larva L3 puffstage 3-6    856 larva L3 puffstage 7-9    888 white prepupae new    990 white prepupae 12hr    1219 white prepupae 24hr    1406 pupae 2d postWPP    960 pupae 3d postWPP    455 pupae 4d postWPP    579 adult male 01day    506 adult male 05day    705 adult male 30day    729 adult female 01day    2109 adult female 05day    3689 adult female 30day    3811 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (6045) embryo 02-04hr  (2669) embryo 04-06hr  (2510) embryo 06-08hr  (2217) embryo 08-10hr  (1607) embryo 10-12hr  (998) embryo 12-14hr  (952) embryo 14-16hr  (879) embryo 16-18hr  (527) embryo 18-20hr  (339) embryo 20-22hr  (247) embryo 22-24hr  (271) larva L1  (607) larva L2  (465) larva L3 12hr old  (370) larva L3 puffstage 1-2  (385) larva L3 puffstage 3-6  (856) larva L3 puffstage 7-9  (888) white prepupae new  (990) white prepupae 12hr  (1219) white prepupae 24hr  (1406) pupae 2d postWPP  (960) pupae 3d postWPP  (455) pupae 4d postWPP  (579) adult male 01day  (506) adult male 05day  (705) adult male 30day  (729) adult female 01day  (2109) adult female 05day  (3689) adult female 30day  (3811) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (6045) embryo 02-04hr  (2669) embryo 04-06hr  (2510) embryo 06-08hr  2217 embryo 08-10hr  1607 embryo 10-12hr    998 embryo 12-14hr    952 embryo 14-16hr    879 embryo 16-18hr    527 embryo 18-20hr    339 embryo 20-22hr    247 embryo 22-24hr    271 larva L1    607 larva L2    465 larva L3 12hr old    370 larva L3 puffstage 1-2    385 larva L3 puffstage 3-6    856 larva L3 puffstage 7-9    888 white prepupae new    990 white prepupae 12hr    1219 white prepupae 24hr  1406 pupae 2d postWPP    960 pupae 3d postWPP    455 pupae 4d postWPP    579 adult male 01day    506 adult male 05day    705 adult male 30day    729 adult female 01day  2109 adult female 05day  (3689) adult female 30day  (3811) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    6045 embryo 02-04hr    2669 embryo 04-06hr    2510 embryo 06-08hr    2217 embryo 08-10hr    1607 embryo 10-12hr    998 embryo 12-14hr    952 embryo 14-16hr    879 embryo 16-18hr    527 embryo 18-20hr    339 embryo 20-22hr    247 embryo 22-24hr    271 larva L1    607 larva L2    465 larva L3 12hr old    370 larva L3 puffstage 1-2    385 larva L3 puffstage 3-6    856 larva L3 puffstage 7-9    888 white prepupae new    990 white prepupae 12hr    1219 white prepupae 24hr    1406 pupae 2d postWPP    960 pupae 3d postWPP    455 pupae 4d postWPP    579 adult male 01day    506 adult male 05day    705 adult male 30day    729 adult female 01day    2109 adult female 05day    3689 adult female 30day    3811 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    6045 embryo 02-04hr    2669 embryo 04-06hr    2510 embryo 06-08hr    2217 embryo 08-10hr    1607 embryo 10-12hr    998 embryo 12-14hr    952 embryo 14-16hr    879 embryo 16-18hr    527 embryo 18-20hr    339 embryo 20-22hr    247 embryo 22-24hr    271 larva L1    607 larva L2    465 larva L3 12hr old    370 larva L3 puffstage 1-2    385 larva L3 puffstage 3-6    856 larva L3 puffstage 7-9    888 white prepupae new    990 white prepupae 12hr    1219 white prepupae 24hr    1406 pupae 2d postWPP    960 pupae 3d postWPP    455 pupae 4d postWPP    579 adult male 01day    506 adult male 05day    705 adult male 30day    729 adult female 01day    2109 adult female 05day    3689 adult female 30day    3811 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0003044    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0003044 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval/adult central nervous system, larval salivary gland, adult ovary. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0003044 expression level Tissue   Expression Level Larval Central Nervous System    280.4 Larval Midgut    53.1 Larval Hindgut    62.6 Larval Malpighian Tubules    56 Larval Fat Body    64.5 Larval Salivary Gland    137.7 Larval Trachea    89.65 Larval Carcass    66.875 Adult Head    39.1 Adult Eye    50.6 Adult Brain    118.9 Adult Thoracic-Abdominal Ganglion    117.4 Adult Crop    57.5 Adult Midgut    41.2 Adult Hindgut    36.7 Adult Malpighian Tubules    44.5 Adult Fat Body    60.5 Adult Salivary Gland    54.8 Adult Heart    31.45 Adult VirginFemale Spermatheca    43.8 Adult InseminatedFemale Spermatheca    40.7 Adult Ovary    354 Adult Testis    97.3 Adult Male Accessory Gland    37 Adult Carcass    39.9 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    280.4 Larval Midgut    53.1 Larval Hindgut    62.6 Larval Malpighian Tubules    56 Larval Fat Body    64.5 Larval Salivary Gland    137.7 Larval Trachea    89.65 Larval Carcass    66.875 Adult Head    39.1 Adult Eye    50.6 Adult Brain    118.9 Adult Thoracic-Abdominal Ganglion    117.4 Adult Crop    57.5 Adult Midgut    41.2 Adult Hindgut    36.7 Adult Malpighian Tubules    44.5 Adult Fat Body    60.5 Adult Salivary Gland    54.8 Adult Heart    31.45 Adult VirginFemale Spermatheca    43.8 Adult InseminatedFemale Spermatheca    40.7 Adult Ovary    354 Adult Testis    97.3 Adult Male Accessory Gland    37 Adult Carcass    39.9 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    280.4 Larval Midgut    53.1 Larval Hindgut    62.6 Larval Malpighian Tubules    56 Larval Fat Body    64.5 Larval Salivary Gland    137.7 Larval Trachea    89.65 Larval Carcass    66.875 Adult Head    39.1 Adult Eye    50.6 Adult Brain    118.9 Adult Thoracic-Abdominal Ganglion    117.4 Adult Crop    57.5 Adult Midgut    41.2 Adult Hindgut    36.7 Adult Malpighian Tubules    44.5 Adult Fat Body    60.5 Adult Salivary Gland    54.8 Adult Heart    31.45 Adult VirginFemale Spermatheca    43.8 Adult InseminatedFemale Spermatheca    40.7 Adult Ovary    354 Adult Testis    97.3 Adult Male Accessory Gland    37 Adult Carcass    39.9 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    280.4 Larval Midgut    53.1 Larval Hindgut    62.6 Larval Malpighian Tubules    56 Larval Fat Body    64.5 Larval Salivary Gland    137.7 Larval Trachea    89.65 Larval Carcass    66.875 Adult Head    39.1 Adult Eye    50.6 Adult Brain    118.9 Adult Thoracic-Abdominal Ganglion    117.4 Adult Crop    57.5 Adult Midgut    41.2 Adult Hindgut    36.7 Adult Malpighian Tubules    44.5 Adult Fat Body    60.5 Adult Salivary Gland    54.8 Adult Heart    31.45 Adult VirginFemale Spermatheca    43.8 Adult InseminatedFemale Spermatheca    40.7 Adult Ovary    354 Adult Testis    97.3 Adult Male Accessory Gland    37 Adult Carcass    39.9 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0003044 expression level Tissue   Expression Level Larval Central Nervous System    280.4 Larval Midgut    53.1 Larval Hindgut    62.6 Larval Malpighian Tubules    56 Larval Fat Body    64.5 Larval Salivary Gland    137.7 Larval Trachea    89.65 Larval Carcass    66.875 Adult Head    39.1 Adult Eye    50.6 Adult Brain    118.9 Adult Thoracic-Abdominal Ganglion    117.4 Adult Crop    57.5 Adult Midgut    41.2 Adult Hindgut    36.7 Adult Malpighian Tubules    44.5 Adult Fat Body    60.5 Adult Salivary Gland    54.8 Adult Heart    31.45 Adult VirginFemale Spermatheca    43.8 Adult InseminatedFemale Spermatheca    40.7 Adult Ovary    354 Adult Testis    97.3 Adult Male Accessory Gland    37 Adult Carcass    39.9 Expression Level Scale  None   Low   Moderate   High  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    280.4 Larval Midgut    53.1 Larval Hindgut    62.6 Larval Malpighian Tubules    56 Larval Fat Body    64.5 Larval Salivary Gland    137.7 Larval Trachea    89.65 Larval Carcass    66.875 Adult Head    39.1 Adult Eye    50.6 Adult Brain    118.9 Adult Thoracic-Abdominal Ganglion    117.4 Adult Crop    57.5 Adult Midgut    41.2 Adult Hindgut    36.7 Adult Malpighian Tubules    44.5 Adult Fat Body    60.5 Adult Salivary Gland    54.8 Adult Heart    31.45 Adult VirginFemale Spermatheca    43.8 Adult InseminatedFemale Spermatheca    40.7 Adult Ovary    354 Adult Testis    97.3 Adult Male Accessory Gland    37 Adult Carcass    39.9 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    280.4 Larval Midgut    53.1 Larval Hindgut    62.6 Larval Malpighian Tubules    56 Larval Fat Body    64.5 Larval Salivary Gland    137.7 Larval Trachea    89.65 Larval Carcass    66.875 Adult Head    39.1 Adult Eye    50.6 Adult Brain    118.9 Adult Thoracic-Abdominal Ganglion    117.4 Adult Crop    57.5 Adult Midgut    41.2 Adult Hindgut    36.7 Adult Malpighian Tubules    44.5 Adult Fat Body    60.5 Adult Salivary Gland    54.8 Adult Heart    31.45 Adult VirginFemale Spermatheca    43.8 Adult InseminatedFemale Spermatheca    40.7 Adult Ovary    354 Adult Testis    97.3 Adult Male Accessory Gland    37 Adult Carcass    39.9 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    280.4 Larval Midgut    53.1 Larval Hindgut    62.6 Larval Malpighian Tubules    56 Larval Fat Body    64.5 Larval Salivary Gland    137.7 Larval Trachea    89.65 Larval Carcass    66.875 Adult Head    39.1 Adult Eye    50.6 Adult Brain    118.9 Adult Thoracic-Abdominal Ganglion    117.4 Adult Crop    57.5 Adult Midgut    41.2 Adult Hindgut    36.7 Adult Malpighian Tubules    44.5 Adult Fat Body    60.5 Adult Salivary Gland    54.8 Adult Heart    31.45 Adult VirginFemale Spermatheca    43.8 Adult InseminatedFemale Spermatheca    40.7 Adult Ovary    354 Adult Testis    97.3 Adult Male Accessory Gland    37 Adult Carcass    39.9 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    39.1   NA  Eye    50.6   NA  Brain    118.9   280.4  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    117.4   NA  Crop    57.5   53.1  Midgut    41.2   62.6  Hindgut    36.7   56  Malpighian Tubules    44.5   64.5  Fat Body    60.5   137.7  Salivary Gland    54.8   NA  Heart    31.45   89.65  Trachea    NA   NA  VirginFemale Spermatheca    43.8   NA  InseminatedFemale Spermatheca    40.7   NA  Ovary    354   NA  Testis    97.3   NA  Male Accessory Gland    37   66.875  Carcass    39.9 modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_06
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0003044 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG5109-RA FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for Pcl Stages(s) 1-3 Stages(s) 4-6 Stages(s) 7-8 Stages(s) 11-12 Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal Pcl5 lethal, with Scer\GAL41407 PclGD11537 lethal | embryonic stage | recessive Pcl1 Pcl216 Pcl372 Pclunspecified lethal | pupal stage, with Scer\GAL4pnr-MD237 PclGD11537 lethal | recessive Pcl2 Pcl9 Pcl18 Pclk08920 Pcls1859 viable Pcl3 Pcl4 Pcl17 Other Phenotypes Allele neuroanatomy defective | larval stage, with Scer\GAL41407 PclGD11537 non-enhancer of variegation, with P{hsp26-pt-T}39C-5 Pcl11 Pcl13 Pcl15 non-enhancer of variegation, with P{hsp26-pt-T}39C-31 Pcl15 non-enhancer of variegation, with P{wA}4-4 Pcl13 Pcl15 non-suppressor of variegation, with P{hsp26-pt-T}39C-5 Pcl11 Pcl13 Pcl15 non-suppressor of variegation, with P{hsp26-pt-T}39C-31 Pcl15 non-suppressor of variegation, with P{wA}4-4 Pcl13 Pcl15 suppressor of variegation | dominant, with P{hsp26-pt-T}39C-27 Pcl13 Pcl15 suppressor of variegation | dominant, with P{hsp26-pt-T}39C-31 Pcl13 suppressor of variegation | dominant, with P{SUPor-P.PRE#6}4 Pcl5 visible Pcl9 visible | adult stage | dominant Pcl10 visible | dominant Pcl1 Pcl2 Pcl3 Pcl4 Pcl6 Pcl7 Pcl11 Pclunspecified visible | dominant | homeotic Pcl1 Pcl10 Pcl11 visible | homeotic (with Pcl1) Pcl4 visible | homeotic (with Pcl4) Pcl1 visible | somatic clone Pcl22M21 Pcl27O4 Pcl27T7 wild-type Pcl+t16.3 Pcl+tcos8.1 Phenotype manifest in Allele abdominal tergite 1 Pcl1 Pcl3 abdominal tergite 2 Pcl1 Pcl3 abdominal tergite 3 Pcl1 Pcl3 abdominal tergite 4 Pcl1 Pcl3 abdominal tergite 4 | male Pcl1 abdominal tergite 5 Pcl1 Pcl3 abdominal tergite 6 Pcl1 Pcl3 abdominal tergite 7 Pcl1 Pcl3 adult abdominal segment 2 | somatic clone Pcl1 adult abdominal segment 3 | somatic clone Pcl1 adult abdominal segment 4 Pcl1 Pcl2 Pcl3 Pcl4 adult abdominal segment 4 | somatic clone Pcl1 adult abdominal segment 5 | ectopic Pcl1 adult abdominal segment 5 | somatic clone Pcl1 adult abdominal segment 6 | somatic clone Pcl1 antenna Pcl1 Pcl3 embryonic/larval neuroblast | larval stage, with Scer\GAL41407 PclGD11537 embryonic abdominal segment 1 Pclunspecified embryonic abdominal segment 2 Pclunspecified embryonic abdominal segment 3 Pclunspecified embryonic abdominal segment 4 Pclunspecified embryonic abdominal segment 5 Pclunspecified embryonic abdominal segment 6 Pclunspecified embryonic abdominal segment 7 Pclunspecified embryonic abdominal segment 8 Pclunspecified embryonic abdominal segment 9 Pclunspecified embryonic abdominal segment 10 Pclunspecified embryonic abdominal segment 11 Pclunspecified female accessory gland Pcl1 Pcl3 ganglion mother cell | larval stage, with Scer\GAL41407 PclGD11537 larval abdominal segment 1 Pcl1 larval abdominal segment 1 | germline clone Pcl1 larval abdominal segment 2 Pcl1 larval abdominal segment 2 | ectopic Pcl1 larval abdominal segment 2 | germline clone Pcl1 larval abdominal segment 3 Pcl1 larval abdominal segment 3 | ectopic Pcl1 larval abdominal segment 3 | germline clone Pcl1 larval abdominal segment 4 Pcl1 larval abdominal segment 4 | ectopic Pcl1 larval abdominal segment 4 | germline clone Pcl1 larval abdominal segment 5 Pcl1 larval abdominal segment 5 | ectopic Pcl1 larval abdominal segment 5 | germline clone Pcl1 larval abdominal segment 6 Pcl1 larval abdominal segment 6 | ectopic Pcl1 larval abdominal segment 6 | germline clone Pcl1 larval abdominal segment 7 Pcl1 larval abdominal segment 7 | ectopic Pcl1 larval abdominal segment 7 | germline clone Pcl1 larval abdominal segment 8 | ectopic Pcl1 larval abdominal segment 8 | ectopic | germline clone Pcl1 larval mesothoracic segment | germline clone Pcl1 larval metathoracic segment | germline clone Pcl1 larval prothoracic segment | germline clone Pcl1 mesothoracic leg Pcl1 Pcl10 Pcl11 Pcl12 Pcl17 Pcl2 Pcl3 Pcl4 Pcl9 Pclunspecified mesothoracic leg (with Pcl1) Pcl4 mesothoracic leg (with Pcl4) Pcl1 mesothoracic leg & sex comb | ectopic Pcl11 mesothoracic leg | anterior compartment Pcl1 Pcl3 mesothoracic leg | posterior compartment Pcl1 Pcl3 metathoracic coxa | anterior compartment Pcl1 Pcl3 metathoracic femur | posterior compartment Pcl1 Pcl3 metathoracic leg Pcl1 Pcl10 Pcl11 Pcl12 Pcl17 Pcl2 Pcl3 Pcl4 Pcl9 Pclunspecified metathoracic leg | anterior compartment Pcl1 Pcl3 metathoracic leg | posterior compartment Pcl1 Pcl3 metathoracic metatarsus | anterior compartment Pcl1 Pcl3 metathoracic trochanter | posterior compartment Pcl1 Pcl3 midgut constriction Pcl12 prothoracic leg | ectopic (with Pcl1) Pcl4 prothoracic leg | ectopic (with Pcl4) Pcl1 sex comb | ectopic Pcl1 Pcl11 sex comb | ectopic (with Pcl1) Pcl4 sex comb | ectopic (with Pcl4) Pcl1 wing Pcl9 wing | somatic clone Pcl1 Pcl22M21 Pcl27O4 Pcl27T7 wing vein L5 Pcl1 Pcl3
Classical Alleles ( 34 )
For All Classical Alleles Show
Allele of Pcl Class Mutagen Stocks Known lesion Pcl11 amorphic allele - genetic evidence gamma ray 2 -- Pcl5 amorphic allele - genetic evidence ethyl nitrosourea 1 -- Pcl13 X ray 1 -- Pcl15 X ray 1 -- Pcl7 ethyl methanesulfonate 1 -- Pclc06923 piggyBac transposase 1 -- Pclc07126 piggyBac transposase 1 -- PclEY08457 P-element activity 1 -- PclGE15295 1 -- Pcls1859 P-element activity 1 -- Pcl10 amorphic allele - genetic evidence gamma ray 0 -- Pcl1 amorphic allele - genetic evidence X ray ethyl methanesulfonate 0 -- Pcl2 amorphic allele - genetic evidence ethyl methanesulfonate 0 -- Pcl12 loss of function allele, hypomorphic allele - genetic evidence gamma ray 0 Yes Pcl14 X ray 0 -- Pcl16 X ray 0 -- Pcl17 X ray ethyl methanesulfonate 0 -- Pcl18 ethyl methanesulfonate 0 -- Pcl216 ethyl methanesulfonate 0 -- Pcl22M21 ethyl methanesulfonate 0 -- Pcl27O4 ethyl methanesulfonate 0 -- Pcl27T7 ethyl methanesulfonate 0 -- Pcl372 ethyl methanesulfonate 0 -- Pcl3 ethyl methanesulfonate 0 -- Pcl4 ethyl methanesulfonate 0 -- Pcl6 ethyl methanesulfonate 0 -- Pcl8 ethyl methanesulfonate 0 -- Pcl9 0 -- Pclk08920 P-element activity 0 -- PclP1 P-element activity 0 Yes PclP2 P-element activity 0 Yes PclR5 0 -- PclT gamma ray 0 -- Pclunspecified 0 --
Alleles Carried on Transgenic Constructs ( 6 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Pcl Class Mutagen Stocks Known lesion PclGD11537 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes PclHMS00896 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes PclHMS00897 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes PclJF01704 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Pcl+t16.3 in vitro construct - genomic fragment 0 -- Pcl+tcos8.1 in vitro construct - genomic fragment 0 --
Aneuploid Aberrations
Disrupted in	Df(2R)Exel7152 (Ryder, 2004.4.26) Df(2R)PC4 (Jurgens, 1985, BDGP Project Members, 1994-1999, Lonie et al., 1994, Harbecke and Lengyel, 1995, Tearle, 1996.5.14) Df(2R)Pcl7B (Spradling et al., 1999, Duncan, 1982, Sato et al., 1983, Tearle and Nusslein-Volhard, 1987, BDGP Project Members, 1994-1999, Soto et al., 1995, Tearle, 1996.5.14) Df(2R)Pcl11B (Duncan, 1982, Sato et al., 1983, Tearle and Nusslein-Volhard, 1987, Harbecke and Lengyel, 1995, Tearle, 1996.5.14) Df(2R)PclXM82 (Tearle, 1996.5.14) Df(2R)Pcl-W5 (Sato et al., 1983, Cheng et al., 1994) In(2R)Pcl11 (Doane, 1999, Doane, 2000)
Not disrupted in	Df(2R)lolal311a (Faucheux et al., 2001)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD11537} NA P{TRiP.HMS00896} NA P{TRiP.HMS00897} NA P{TRiP.JF01704} NA characterization construct P{cos8.1} NA P{stau+16.3} NA
Insertions
	Type of insertions Name Expression data insertion of enhancer trap P{lacW}l(2)SH0453SH0453 No P{lacW}Pclk08920 No P{lacW}Pcls1859 No insertion of mobile activating element P{EP}PclGE15295 NA P{EPgy2}PclEY08457 NA miscellaneous insertions P{ry11}PclP1 NA P{ry11}PclP2 NA PBac{PB}Pclc06923 NA PBac{PB}Pclc07126 NA
Gene Ontology: Function, Process & Cellular Component ( 7 unique terms )
Terms Based on Experimental Evidence ( 6 terms )
Molecular Function
CV term Evidence References
chromatin binding inferred from direct assay (Lonie et al., 1994) protein binding inferred from physical interaction with E(z) (assigned by UniProtKB) (O'Connell et al., 2001) inferred from physical interaction with Rpd3 AND inferred from physical interaction with Su(z)12 AND inferred from physical interaction with esc (assigned by UniProtKB) (Tie et al., 2003) sequence-specific DNA binding transcription factor activity inferred from direct assay (assigned by UniProtKB) (O'Connell et al., 2001, Tie et al., 2003)
Biological Process
CV term Evidence References
negative regulation of transcription, DNA-dependent inferred from direct assay (assigned by UniProtKB) (Tie et al., 2003) neurogenesis inferred from mutant phenotype (Neumüller et al., 2011)
Cellular Component
CV term Evidence References
nucleus inferred from direct assay (assigned by UniProtKB) (Lonie et al., 1994)
Terms Based on Predictions or Assertions ( 2 terms )
Molecular Function
CV term Evidence References
zinc ion binding inferred from electronic annotation with InterPro:IPR001965 (FlyBase Curators et al., 2004-)
Biological Process ( 0 terms)
Cellular Component
CV term Evidence References
nucleus inferred from sequence or structural similarity with NCBI_gi:998846 (FlyBase, 1992-) non-traceable author statement (Lonie, 1995.3.28)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Pcl allele Gene References Pcl1 Abd-B (Duncan, 1982) Antp (Duncan, 1982) Gug (Kankel et al., 2004) Hsc70-4 (Mollaaghababa et al., 2001) Scr (Duncan, 1982) Ubx (Duncan, 1982) Pcl4 Gug (Kankel et al., 2004) Pcl10 Abd-B (Mihaly et al., 1997) gpp (Shanower et al., 2005) mor (Brizuela and Kennison, 1997) Pc (Kehle et al., 1998) w (Muller et al., 1999, Cavalli, 1999) Pcl11 corto (Lopez et al., 2001) Sir2 (Furuyama et al., 2004) Pcl15 corto (Lopez et al., 2001) mxc (Docquier et al., 1996, Saget et al., 1998) w (Netter et al., 1998) Pcl18 Pc (Kennison and Tamkun, 1988) Pcl+t16.3 Pc (Campbell et al., 1995) PclR5 E(z) (Bajusz et al., 2001) Pclunspecified brm (Tamkun et al., 1992) skd (Lindsley and Zimm, 1992) Su(z)12 (Birve and Rasmuson-Lestander, 1994) unspecified Abd-B (Muller et al., 1999) Antp (Hannah-Alava, 1964, Kennison and Tamkun, 1988) ara (Netter et al., 1998) ash1 (Gildea et al., 2000) Asx (Campbell et al., 1995) E(Pc) (Lindsley and Zimm, 1992, Campbell et al., 1995) E(z) (Campbell et al., 1995) esc (Campbell et al., 1995) Fab-7 (Cavalli, 1999) osa (Lindsley and Zimm, 1992) Pc (Duncan, 1982) Pcl (Nusslein-Volhard, 1992) Psc (Campbell et al., 1995) rept (Qi et al., 2006) Sce (Campbell et al., 1995) Scm (Campbell et al., 1995) Scr (Gindhart and Kaufman, 1995) trx (Gildea et al., 2000)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 62752 DroID - A comprehensive database of gene and protein interactions. • FBgn0003044 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 37069
Orthologs
Genome-wide drosophilid orthologs	Dana\GF12651 Dere\GG21845 Dgri\GH21051 Dmoj\GI18653 Dper\GL11547 Dpse\GA18665 Dsec\GM21846 Dsim\GD11340 Dvir\GJ21669 Dwil\GK19650 Dyak\GE11924
Curated drosophilid orthologs	Dafs\Pcl (Bartolome and Charlesworth, 2006) Dmir\Pcl (Bartolome and Charlesworth, 2006, Bartolome and Charlesworth, 2006) Dpse\GA18665 (Bartolome and Charlesworth, 2006)
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • XP_396733.2 Apis mellifera (honey bee) XP_001949615.1 Acyrthosiphon pisum (pea aphid) ENSBTAP00000001831 Bos taurus (bovine) ENSCAFP00000029874 Canis familiaris (dog) ENSCINP00000026271 Ciona intestinalis (sea squirt) CPIJ005572 Culex pipens (mosquito) ENSCPOP00000002036 Cavia porcellus (guinea pig) 187445 Capitella spl (polychaete worm) 301671 Daphnia pulex (daphnia) ENSDARP00000091407 Danio rerio (zebra fish) ENSECAP00000015346 Equus caballus (horse) ENSGACP00000014147 Gymnopilus aculeatus (mushroom) ENSGALP00000009493 Gallus gallus (chicken) ENSP00000359321 Homo sapiens (human) ISCW018780-PA Ixodes scapularis (deer tick) ENSMODP00000006119 Monodelphis domestica (gray opossum) ENSMMUP00000022097 Macaca mulatta (rhesus monkey) ENSMUSP00000108247 Mus musculus (house mouse) ENSORLP00000006459 Oryzias latipes (japanese killifish) ENSPPYP00000001332 Pongo pygmaeus (orangutan) ENSPTRP00000052062 Pan troglodytes (chimpanzee) ENSRNOP00000000085 Rattus norvegicus (norway rat) XP_794791.2 Strongylocentrotus purpuratus (purple sea urchin) 56605 Trichoplax adhaerens (metazoa) XP_972200.1 Tribolium castaneum (red flour beetle) ENSTNIP00000005027 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000046767 Takifugu rubripes (pufferfish Takifugu) ENSXETP00000049746 Xenopus tropicalis (frog) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB13940 NV14568 LH21649 ISCW018780 FBgn0229706 FBgn0227541 FBgn0221648 FBgn0208788 FBgn0183089 FBgn0176723 JGI_V11_301671 FBgn0078665 FBgn0149156 FBgn0141392 FBgn0128513 FBgn0114023 FBgn0089687 CPIJ005572 BGIBMGA007840 ACYPI008514 GB16657 AGAP001019 AAEL010211 PHUM339230 GLEAN_04391
Stocks & Reagents
Stocks Listed in FlyBase ( 16 )
Bloomington	26893 w*; P{EP}PclGE15295 2401 In(2R)Pcl11, Pcl11/SM5 24157 w*; P{neoFRT}42D Pcl5/CyO 12058 w1118; P{lacW}Pcls1859/CyO 31189 y1 v1; P{TRiP.JF01704}attP2 33945 y1 sc* v1; P{TRiP.HMS00896}attP2 19876 y1 w67c23; P{EPgy2}PclEY08457/CyO
Harvard	- PBac{PB}Pclc06923 - PBac{PB}Pclc07126
Kyoto	107312 In(2R)Pcl11, Pcl11/SM5
VDRC	v22004 w1118; P{GD11537}v22004 v22005 w1118; P{GD11537}v22005
	More stocks available...
Genomic Clones ( 1 )
	BACR33K09 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 112 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	SD09488
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT04403 AT15011 AT15383 AT16226 AT18079 AT20804 AT25491 AT30678 GH02674 GH22981 GM05071 HL01608 HL02632 LD23632 LD25643 LD28226 LP24231 RE03638 RE07181 RE39677 RE55236 SD20480
Other clones	15912 65521 67800 70224 78122 99112 104026 106681 214379.55 AT23239 AT29040 CK01.3A.D4 CK01.4C.C6 CK01.4C.C8 CK01.10C.D11 CK01.23C.F1 CK01.77A.F6 CK01.100A.D4 CK01.141D.E2 EC068152 EK043916 EK060255 EK118049 EK126236 EK195851 EK197642 EK227251 EK228029 EK232462 EK281701 EK316741 EP18416 LD38218 RP003072250 RP003075878 RP003076867 RP003078440 RP003086416 RP003100019 RP003112219 RP003129029 RP003138042 RP003140476 RP003145768 RP003179369 RP003183264 RP003183512 RP003201750 RP003202006 RP003209108 RP003224041 RP003245034 RP003247060 RP003250903 RP003261583 RP003262243 RP003270635 RP003276639 RP003279694 RP003281002 RP003295537 RP003319543 RP003330649 RP003334503 RP003337961 RP003350379 RP003350596 RP003351369 RP003394054 RP003395448 RP003400073 RP003403914 RP003424392 RP003424483 RP003441262 RP003448518 RP003493282 RP003508138 RP003518650 RP003539172 RP003541477 RP003584384 RP003590879 RP003609167 RP003640175 RP003678279 RP004243959 SD02238 SD08312
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0003044 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 37069
Antibody Information
	polyclonal (Lonie et al., 1994, Tie et al., 2003)
Other Information
Discoverer
	Duncan. (Lindsley and Zimm, 1992)
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: Pcl l(2)s1859 (Spradling et al., 1999)
Additional comments
Other Comments
	DNA-protein interactions: genome-wide binding profile assayed for Pcl protein in Kc167 cells; see Chromatin_types_NKI collection report. Individual protein-binding experiments listed under "Samples" at GEO_GSE22069 (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE22069). (Filion et al., 2010) Abd-B MCP725 element is a silencer that functions throughout proliferation of the imaginal discs. MCP725-mediated silencing requires the Pc and Pcl proteins (it is likely that other members of the PcG also interact with the MCP725 silencer). (Busturia et al., 1997) Silencing activity of the iab-7PRE in the bithorax complex is dependent upon proteins from the Polycomb group. (Hagstrom et al., 1997) In an effort to subdivide the Pc-group genes functionally, the phenotypes of adult flies heterozygous for every pairwise combination of Pc-group mutation were examined. Asx, Pc, Pcl, Psc, Scm and Sce have similar functions in some imaginal tissues. Genetic interactions have been demonstrated between esc, Asx, E(Pc), Pcl, E(z) and sxc. Most duplications of Pc-group genes neither exhibit anterior transformations nor suppress the extra sex comb phenotype of Pc-group mutations, suggesting that not all Pc-group genes behave as predicted by the mass action model. (Campbell et al., 1995) Sections of the Scr regulatory region may be important for regulation of Scr by Polycomb- and trithorax-group genes. (Gindhart and Kaufman, 1995) Mosaic and expression pattern analysis reveals that the Pc-group genes do not act only in a common complex or pathway: they must have some independent functions. (Soto et al., 1995) Duplication of ph-d suppress homeotic transformations of Pc and Pcl, this data supports the conclusion that ph-d is at equilibrium with a multimeric complex containing Pc group genes. (Cheng et al., 1994) Alleles of trx lighten the eye colour of some pairing sensitive allele of w, whereas alleles of Pcl darken their eye colour. (Kassis, 1994) Pcl product is found on larval salivary chromosomes at about 100 specific loci, in the same positions as the products of Pc, ph-d and ph-p genes. (Lonie et al., 1994) The bithorax complex genes are regulated by the Pc group of genes, acting via 'Pc group response elements' (PREs), that can work even when removed from the normal bithorax complex context. The Pc group products apparently provide stable memory or imprinting of boundaries which are specified by gap and pair-rule regulators. (Simon et al., 1993) Strong Pcl mutant alleles (Pcl1, Pcl2) are homozygous lethal, hemizygous lethal and lethal with each other, the embryos having partial posteriorly-directed transformations of the abdominal segments (segment 1 into segment 2 and segments 2-7 into more posterior segments). These mutants are strong enhancers of Pc3, showing Pc-like transformations in embryos and are indistinguishable from Pcl deficiencies in complementation behavior. Extreme posteriorly-directed segmental transformations are found in Pcl1/Df(2R)Pcl11B; Pc3/Pc3 lethal embryos (FBrf0038059); these embryos are considerably more abnormal than the Pc3 homozygotes that are Pcl+. Pcl/Pcl embryos that are also homozygous for Asx, Psc, or Scm show strong posteriorly-directed transformations of the entire body pattern; the triple mutant Psc, Asx, Pcl has a tandem array of posterior abdominal segments including four abdominal denticle bands in the head region (FBrf0043317). Pcl1/Pcl1 clones in the second through the sixth abdominal tergites also show caudal transformations; similar clones in the wing often appear to be partially transformed into haltere tissue. Weak Pcl alleles (Pcl3, Pcl4) survive, at least through eclosion, either as homozygotes or as heterozygotes with strong or weak Pcl alleles (FBrf0038059). Pcl1/Pcl3 and Pcl1/Pcl4 show segmental transformations characteristic of Pc/+ flies, i.e. antenna to leg, second and third leg to first leg, wing to haltere, and posteriorly-directed abdominal transformations (the most frequent abnormality). Increasing the dosage of the BXC in Pcl1/Pcl4 heterozygotes suppresses the posteriorly-directed transformations, but enhances the transformations from leg2 and leg3 to leg1; increasing the dosage of the ANTC enhances both types of transformations (FBrf0038059). Pcl1/Pcl3 females, which die before or soon after eclosion, show a conversion of parovaria to spermathecae. Both strong and weak Pcl alleles are viable as heterozygotes (Pcl/+) and may show partial abdominal and leg transformations, the abnormalities increasing in Pcl/+;Pc/+ flies or when Pcl/+ is combined with E(Pc). (Lindsley and Zimm, 1992) Pcl+, like Pc+, may be considered a negative regulator of the BXC and the ANTC. (Lindsley and Zimm, 1992) Embryos mutant for two or more Pc group genes (Pc, Scm, Pcl, Psc, Asx, E(Pc), E(z), ph-d, pho and esc) show strong ectopic en expression, but only weak derepression occurs if embryo is mutant at only one of the Pc group genes. This effect is independent of the function of en itself, and wg. (Moazed and O'Farrell, 1992) Mutations of genes in the polycomb group (esc, E(z), Pc, ph-p, ph-d, Scm, Pcl, Sce, Asx, Psc, pho and Antp) cause abnormal segmental development due to the ectopic expression of abd-A and Abd-B. Embryos lacking both maternal and zygotic Pcl product were generated to determine abd-A and Abd-B expression patterns. (Simon et al., 1992) Mutations in Pcl derepress homeotic genes in the BXC and ANTC. (Tamkun et al., 1992) The Pc group genes are negative regulators of homeotic genes. (McKeon and Brock, 1991) Pcl is one of the 18 loci identified in a screen for dominant modifiers of Pc and/or Antp phenotypes. Alleles of Pc, Pcl, Scm, Dll, brm, kto, Scr and trx show clear dominant enhancement or suppression of AntpScx, whereas alleles of vtd, Vha55, Su(Pc)37D, urd, mor, skd and osa do not. (Kennison and Tamkun, 1988) Mutants of Pcl exhibit a reduction in sex comb teeth on the second and third legs. (Kennison and Russell, 1987) Pole cell transplantation techniques demonstrate that Pcl is maternally expressed and is required for normal BXC expression during embryogenesis. (Breen and Duncan, 1986)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_057324 RefSeq (Proteins) • NP_476672 DDBJ / EMBL / GenBank • AAA64457 AAF57748 AAL68389 AC004296 AI063158 AI542964 AQ026142 AW944600 AY075585 CL705829 CZ471085 CZ471264 L35153 Z31895 Z32189 Entrez Gene - A searchable database of RefSeq genes. • 37069 UniProtKB/Swiss-Prot • Q24459
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Zinc finger, PHD-type (IPR001965) Tudor domain (IPR002999) Zinc finger, FYVE/PHD-type (IPR011011) Zinc finger, PHD-type, conserved site (IPR019786) Zinc finger, PHD-finger (IPR019787) Zinc finger, RING/FYVE/PHD-type (IPR013083)
Linkouts
	BioGRID - A database of protein and genetic interactions • 62752 DroID - A comprehensive database of gene and protein interactions. • FBgn0003044 DRSC - Results from RNAi screens. • FBgn0003044 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0003044 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG5109-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0003044 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 37069 InParanoid A subset of ortholog calls from InParanoid. • XP_396733.2 Apis mellifera (honey bee) XP_001949615.1 Acyrthosiphon pisum (pea aphid) ENSBTAP00000001831 Bos taurus (bovine) ENSCAFP00000029874 Canis familiaris (dog) ENSCINP00000026271 Ciona intestinalis (sea squirt) CPIJ005572 Culex pipens (mosquito) ENSCPOP00000002036 Cavia porcellus (guinea pig) 187445 Capitella spl (polychaete worm) 301671 Daphnia pulex (daphnia) ENSDARP00000091407 Danio rerio (zebra fish) ENSECAP00000015346 Equus caballus (horse) ENSGACP00000014147 Gymnopilus aculeatus (mushroom) ENSGALP00000009493 Gallus gallus (chicken) ENSP00000359321 Homo sapiens (human) ISCW018780-PA Ixodes scapularis (deer tick) ENSMODP00000006119 Monodelphis domestica (gray opossum) ENSMMUP00000022097 Macaca mulatta (rhesus monkey) ENSMUSP00000108247 Mus musculus (house mouse) ENSORLP00000006459 Oryzias latipes (japanese killifish) ENSPPYP00000001332 Pongo pygmaeus (orangutan) ENSPTRP00000052062 Pan troglodytes (chimpanzee) ENSRNOP00000000085 Rattus norvegicus (norway rat) XP_794791.2 Strongylocentrotus purpuratus (purple sea urchin) 56605 Trichoplax adhaerens (metazoa) XP_972200.1 Tribolium castaneum (red flour beetle) ENSTNIP00000005027 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000046767 Takifugu rubripes (pufferfish Takifugu) ENSXETP00000049746 Xenopus tropicalis (frog) Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /genebrief/polycomblike.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 37069 modMine - Data generated by the modENCODE project. • FBgn0003044 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB13940 NV14568 LH21649 ISCW018780 FBgn0229706 FBgn0227541 FBgn0221648 FBgn0208788 FBgn0183089 FBgn0176723 JGI_V11_301671 FBgn0078665 FBgn0149156 FBgn0141392 FBgn0128513 FBgn0114023 FBgn0089687 CPIJ005572 BGIBMGA007840 ACYPI008514 GB16657 AGAP001019 AAEL010211 PHUM339230 GLEAN_04391
Synonyms & Secondary IDs ( 7 )
Reported As
Symbol Synonym	CG5109 (Klebes et al., 2005, Bienz, 2006, Gonsalvez et al., 2008, Ni et al., 2008, Ni et al., 2010.12.1) l(2)s1859 (Meister and Braun, 1995.10, BDGP Project Members, 1994-1999, ) PCL (Tie, 2002, Schwartz and Pirrotta, 2007, Filion et al., 2010) pcl (Lonie, 1995.3.28, Gonsalvez et al., 2008) Pcl (Chanas and Maschat, 2005, Holmes et al., 2006, Qi et al., 2006, Papp and Muller, 2006, Culi et al., 2006, Bartolome and Charlesworth, 2006, Kavi et al., 2006, Hirose et al., 2001, Muller and Kassis, 2006, Bai et al., 2007, de Ayala Alonso et al., 2007, Ali and Bender, 2004, Nekrasov et al., 2007, Savla et al., 2008, Iampietro et al., 2008, Garaulet et al., 2008, Levis, 2009.3.18, Tie et al., 2003, Bantignies et al., 2011, Khan et al., 2009, Balasov, 2002, Friberg et al., 2010, Kwon et al., 2003, Yatsu et al., 2008, Neumüller et al., 2011, Park et al., 2011)
Name Synonym	Polycomblike (Bienz, 2006, Ali and Bender, 2004, Kwong et al., 2008, Garaulet et al., 2008, Friberg et al., 2010, Kwon et al., 2003, Park et al., 2011) Polycomb-like (Kavi et al., 2006, Muller and Kassis, 2006, Balasov, 2002)
Secondary FlyBase IDs
	FBgn0010678
References ( 195 )
Generate a list of	All references relating to this gene ( 195 )
List References by type	Research paper  ( 115 ) Supplementary material  ( 4 ) Review  ( 32 ) Personal communication to FlyBase  ( 10 ) Abstract  ( 23 ) FlyBase analysis  ( 2 ) DNA/RNA sequence record  ( 1 ) Computer file  ( 4 ) Book  ( 1 ) Stock list  ( 2 ) Curated genome annotation  ( 1 )
Recent research papers ( 6 )
	Bantignies et al., 2011, Cell 144(2): 214--226 Polycomb-Dependent Regulatory Contacts between Distant Hox Loci in Drosophila. [FBrf0212775] Gibert et al., 2011, PLoS Genet. 7(1): e1001280 Segregating Variation in the Polycomb Group Gene cramped Alters the Effect of Temperature on Multiple Traits. [FBrf0212864] Neumüller et al., 2011, Cell Stem Cell 8(5): 580--593 Genome-Wide Analysis of Self-Renewal in Drosophila Neural Stem Cells by Transgenic RNAi. [FBrf0213621] Park et al., 2011, PLoS Genet. 7(8): e1002241 Specification of Drosophila corpora cardiaca neuroendocrine cells from mesoderm is regulated by Notch signaling. [FBrf0215231] Filion et al., 2010, Cell 143(2): 212--224 Systematic protein location mapping reveals five principal chromatin types in Drosophila cells. [FBrf0212051] Friberg et al., 2010, Protein Sci. 19(10): 1906--1916 Structure of an atypical Tudor domain in the Drosophila Polycomblike protein. [FBrf0211912] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...